REBECCA GEORGINA SMITH

CONTACT INFORMATION

Work Home C2.26 MRC SGDP Centre, 1 Prior’s Way, Institute of Psychiatry, Psychology and Cowden, Neuroscience Kent, King’s College London, TN8 7HX London, SE5 8AF Phone Number: 07595 232 708 Phone Number: 020 7848 5271 Email: [email protected]

BIOGRAPHY I earned a BSc (Hons) in Genetics from the University of Leeds which was completed in 2005. In 2007, I began working at the Social, Genetic and Developmental Psychiatry Centre (SGDP), King’s College London as a research assistant working under Prof Ian Craig, Dr Katherine Aitchison and Prof Peter McGuffin on the GENDEP project in which I performed genotyping of genetic polymorphisms such as SNPs and VNTRs in a large unipolar depression cohort. In 2009, I started my PhD at the SGDP in psychiatric genetics with Prof Jonathan Mill and Prof Ian Craig working on my thesis investigating behavioural and molecular changes associated with advanced paternal age in a mouse model, and also contributing to work on allele-specific methylation and the role of epigenetics in psychiatric disease.

I completed my first post-doctoral research position at the SGDP with Dr Katie Lunnon and Prof Jonathan Mill investigating epigenetic changes associated with Alzheimer’s disease in a post-mortem brain sample, as well as continuing my research in paternal age and psychiatric disease with Dr Cathy Fernandes and Dr Abraham Reichenberg. My second post-doctoral position was working on a genome-wide epigenetic project investigating DNA methylation changes and differences at birth and in childhood, for children with different trajectories of conduct problems. This work used blood samples from a subsample of the Avon Longitudinal Study of Parents and Children (ALSPAC) and was carried out under the supervision of Dr Edward Barker and Prof Mill. During this time, I also started running the SGDP epigenetic collaborative services, where I collaborated with many scientists who were interested in epigenetic research. Through this role, my collaborations have included projects on depression in discordant monozygotic twins, candidate gene analysis in blood samples from THC exposure patients and DNA from multiple brain regions in a mouse model of Alzheimer’s disease. This role includes assisting in the design of the project and to perform costing, carrying out any laboratory work required and providing help with the analysis and interpretation of the results where needed. My current post-doctoral position is performing laboratory work and analysis on a study of post-mortem autism brains, carrying out DNA methylation and 5-hydroxymethylcytosine analysis with Prof Mill and Dr Chloe Wong, as well as continuing my work on the epigenetic collaborative services. REBECCA SMITH PAGE 2

Throughout my post-doctoral positions and my PhD, I was able to perform my own statistical analysis, mostly using the R statistical environment. Using R, I have performed analysis of genotyping data, candidate gene and epigenome-wide association study data, methylation and expression QTLs, performed meta-analysis and used a variety of packages within R for quality control, normalisation and analysis. I have also used many bioinformatics tools for gene-ontology analysis, primer design and gene information tools such as UCSC and galaxy. Throughout my post-doctoral positions, I have also been involved in the teaching of MSc courses, both within and externally to King’s College London and the design of projects and supervision of MSc laboratory project students.

EDUCATION PhD in Psychiatric Genetics Jan 2009 – Jan 2012 MRC Social, Genetic and Developmental Psychiatry Centre, Institute of Psychiatry, Kings College London Behavioural and Molecular Changes Associated with Advanced Paternal Age

BSc Honours in Genetics Sept 2002 – Jun 2005 University of Leeds Grade 2.ii

EMPLOYMENT Lead Collaborative Service Researcher (Part Time) Jan 2014 – Present MRC Social, Genetic and Developmental Psychiatry Centre, Institute of Psychiatry, Kings College London Melissa Sollich and Prof Jonathan Mill

Post-Doctoral Researcher (Part Time) Jan 2015 – Present MRC Social, Genetic and Developmental Psychiatry Centre, Institute of Psychiatry, Kings College London Prof Jonathan Mill – DNA Methylation and 5-hydroxymethylcytosine in Autism Brain samples

Post-Doctoral Researcher (Part Time) Jan 2014 – Dec 2014 MRC Social, Genetic and Developmental Psychiatry Centre, Institute of Psychiatry, Kings College London Dr Edward Barker and Prof Jonathan Mill – Epigenetic Pathways to Conduct Problems (ALSPAC)

Post-Doctoral Researcher Jan 2012 – Dec 2013 MRC Social, Genetic and Developmental Psychiatry Centre, Institute of Psychiatry, Kings College London Prof Jonathan Mill – Epigenetics of Alzheimer’s Disease

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Research Assistant Mar 2007 – Dec 2008 MRC Social, Genetic and Developmental Psychiatry Centre, Institute of Psychiatry, Kings College London Dr Katherine Aitchison – GENDEP Project

Research Assistant Aug 2006 – Dec 2006 MRC Centre for Developmental Neurobiology, Guy’s Hospital, Kings College London Prof Sarah Guthrie

PUBLICATIONS: ResearcherID: C-1978-2012, H-Index: 8

Spiers, H., Hannon, E., Schalkwyk, L.C., Smith, R., Wong, C.C.Y., O’Donovan, M.C., Bray, N.J., Mill, J. (2015) Methylomic trajectories across human fetal brain development. Genome Res.

Lunnon, L & Smith, R.G., Cooper, I., Greenbaum L., Mill J., Beeri, M.S. (2015). Blood methylomic signatures of presymptomatic dementia in elderly subjects with type 2 diabetes mellitus. Neurobiology of Aging 36(3): 1600.

Smith, R. G. , C. Fernandes, R. Kember, L. C. Schalkwyk, J. Buxbaum, A. Reichenberg and J. Mill (2014). Transcriptomic changes in the frontal cortex associated with paternal age. Mol Autism 5(1): 24.

Lunnon, K., R. Smith, E. Hannon, P. L. De Jager, G. Srivastava, M. Volta, C. Troakes, S. Al-Sarraj, J. Burrage, R. Macdonald, D. Condliffe, L. W. Harries, P. Katsel, V. Haroutunian, Z. Kaminsky, C. Joachim, J. Powell, S. Lovestone, D. A. Bennett, L. C. Schalkwyk and J. Mill (2014). Methylomic profiling implicates cortical deregulation of ANK1 in Alzheimer's disease. Nat Neurosci 17(9): 1164-1170.

Hodgson, K., K. Tansey, M. Z. Dernovsek, J. Hauser, N. Henigsberg, W. Maier, O. Mors, A. Placentino, M. Rietschel, D. Souery, R. Smith, I. W. Craig, A. E. Farmer, K. J. Aitchison, S. Belsy, O. S. Davis, R. Uher and P. McGuffin (2014). Genetic differences in cytochrome P450 enzymes and antidepressant treatment response. J Psychopharmacol 28(2): 133-141.

Cecil, C. A., L. J. Lysenko, S. R. Jaffee, J. B. Pingault, R. G. Smith, C. L. Relton, G. Woodward, W. McArdle, J. Mill and E. D. Barker (2014). Environmental risk, Oxytocin Receptor Gene (OXTR) methylation and youth callous-unemotional traits: a 13-year longitudinal study. Mol Psychiatry 19(10): 1071-7.

Smith, R. G. , A. Reichenberg, R. L. Kember, J. D. Buxbaum, L. C. Schalkwyk, C. Fernandes and J. Mill (2013). Advanced paternal age is associated with altered DNA methylation at brain-expressed imprinted loci in inbred mice: implications for neuropsychiatric disease. Mol Psychiatry 18(6): 635-636.

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Powell, T. R., R. G. Smith, S. Hackinger, L. C. Schalkwyk, R. Uher, P. McGuffin, J. Mill and K. E. Tansey (2013). DNA methylation in interleukin-11 predicts clinical response to antidepressants in GENDEP. Transl Psychiatry 3: e300.

Smith, R.G., Reichenberg, A., Kember, R.L., Buxbaum, J.D., Schalkwyk, L.C., Fernandes, C., and Mill, J. (2012). Advanced paternal age is associated with altered DNA methylation at brain-expressed imprinted loci in inbred mice: implications for neuropsychiatric disease. Mol Psych 10.1038/mp.2012.88.

Pidsley, R., C. Fernandes, J. Viana, J. L. Paya-Cano, L. Liu, R. G. Smith, L. C. Schalkwyk and J. Mill (2012). DNA methylation at the Igf2/H19 imprinting control region is associated with cerebellum mass in outbred mice. Mol Brain 5: 42.

Huezo-Diaz, P., Perroud, N., Spencer, E.P., Smith, R., Sim, S., Virding, S., Uher, R., Gunasinghe, C., Gray, J., Campbell, D., et al. (2012). CYP2C19 genotype predicts steady state escitalopram concentration in GENDEP. J Psychopharmacol 26, 398-407. Keers, R., Uher, R., Huezo-Diaz, P., Smith, R., Jaffee, S., Rietschel, M., Henigsberg, N., Kozel, D., Mors, O., Maier, W., et al. (2011). Interaction between serotonin transporter gene variants and life events predicts response to antidepressants in the GENDEP project. Pharmacogenomics J 11, 138-145.

Schalkwyk, L.C., Meaburn, E.L., Smith, R., Dempster, E.L., Jeffries, A.R., Davies, M.N., Plomin, R., and Mill, J. (2010). Allelic skewing of DNA methylation is widespread across the genome. Am J Hum Genet 86, 196- 212.

Drago, A., Serretti, A., Smith, R., Huezo-Diaz, P., Malitas, P., Albani, D., Ronchi, D.D., Pae, C.-U., and Aitchison, K.J. (2010). No association between genetic markers in BDNF gene and lithium prophylaxis in a Greek sample. International Journal of Psychiatry in Clinical Practice 14, 154-157.

Uher, R., Huezo-Diaz, P., Perroud, N., Smith, R., Rietschel, M., Mors, O., Hauser, J., Maier, W., Kozel, D., Henigsberg, N., et al. (2009). Genetic predictors of response to antidepressants in the GENDEP project. Pharmacogenomics J 9, 225-233.

Smith, R.G. & Kember, R.L., Mill, J., Fernandes, C., Schalkwyk, L.C., Buxbaum, J.D., and Reichenberg, A. (2009). Advancing paternal age is associated with deficits in social and exploratory behaviors in the offspring: a mouse model. PLoS One 4, e8456.

Perroud, N., Aitchison, K.J., Uher, R., Smith, R., Huezo-Diaz, P., Marusic, A., Maier, W., Mors, O., Placentino, A., Henigsberg, N., et al. (2009). Genetic predictors of increase in suicidal ideation during antidepressant treatment in the GENDEP project. Neuropsychopharmacology 34, 2517-2528.

Huezo-Diaz, P., Uher, R., Smith, R., Rietschel, M., Henigsberg, N., Marusic, A., Mors, O., Maier, W., Hauser, J., Souery, D., et al. (2009). Moderation of antidepressant response by the serotonin transporter gene. Br J Psychiatry 195, 30-38.

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PAPERS IN PREPARATION/SUBMITTED Smith, R.G. & Lysenko, L., Cecil, C.A.M., Relton, C., Maughan, B. Jaffee, S., Oullet-Morin, I., O’Connor, T.G., Woodward, G., McArdle, W., Mill, Barker, E.D. Epigenetic pathways to conduct problems

Janecka, M., Parsons, M.J., Liu, L., Paya-Cano, J., Smith, R., Fernandes, C. & Schalkwyk, L.C. Genetic polymorphisms and their association with brain and behavioural measures in heterogeneous stock mice.

BOOK CHAPTERS Smith, R., and Mill, J. (2011). Epigenetics and Chronic Diseases: An Overview. In Epigenetic Aspects of Chronic Disease. (Springer).

COURSES Post-Graduate Certificate of Academic Practices October 2013 – Kings College London August 2014

Enhancing Academic Practice Programme October 2012 – Kings College London August 2013

Bioinformatics for Geneticists – Summer School July 2009 MRC Social, Genetic and Developmental Psychiatry Centre, Institute of Psychiatry, Kings College London

Phlebotomy Training June 2009 St Thomas’ Hospital

Home Office Personal Licence Course Jan 2009 Guy’s Hospital

Microarrays and Gene expression – Summer School July 2007 MRC Social, Genetic and Developmental Psychiatry Centre, Institute of Psychiatry, Kings College London

TEACHING Co-Module Lead and Lecturer, Molecular Genetics and Genomics Jan 2014 – Goldsmiths, University of London, March 2014 New Cross, London

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Laboratory Methods Session Lead, MSc Genes, Environment and Development Sept 2013 - Present MRC Social, Genetic and Developmental Psychiatry Centre, Institute of Psychiatry, Kings College London

Personal Tutor for student on Genes, Environment and Development Sept 2013 – Present MRC Social, Genetic and Developmental Psychiatry Centre, Institute of Psychiatry, Kings College London

Second Marker for Examinations on MSc Genes, Environment and Development Sept 2013 - Present MRC Social, Genetic and Developmental Psychiatry Centre, Institute of Psychiatry, Kings College London

Second Marker for Examinations on MSc Neuroscience (Behavioural Genetics) April 2013 - Present MRC Social, Genetic and Developmental Psychiatry Centre, Institute of Psychiatry, Kings College London

Lecturer on Epigenetics on MSc Neuroscience Oct 2012 – Present MRC Social, Genetic and Developmental Psychiatry Centre, Institute of Psychiatry, Kings College London

Personal Tutor for student on MSc Neuroscience Oct 2012 – Present MRC Social, Genetic and Developmental Psychiatry Centre, Institute of Psychiatry, Kings College London

Project Supervisor for project on MSc Neuroscience – Simon Lott January – August 2014 MRC Social, Genetic and Developmental Psychiatry Centre, Institute of Psychiatry, Kings College London

Project Supervisor for project on MSc Neuroscience – Amandeep Virk April – August 2012 MRC Social, Genetic and Developmental Psychiatry Centre, Institute of Psychiatry, Kings College London

Introduction to Molecular Methods in Functional Genomics – Summer School June 2012 MRC Social, Genetic and Developmental Psychiatry Centre, Institute of Psychiatry, Kings College London Demonstrator

Introduction to Molecular Methods in Functional Genomics – Summer School July 2010 MRC Social, Genetic and Developmental Psychiatry Centre, Institute of Psychiatry, Kings College London Demonstrator

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Introduction to Molecular Methods in Functional Genomics – Summer School July 2008 MRC Social, Genetic and Developmental Psychiatry Centre, Institute of Psychiatry, Kings College London Demonstrator

COLLEGIAL ACTIVITIES Member of Animal Welfare and Ethical Review Body (AWERB) May 2014 - Present

Post-Doctoral Forum (“Your Network”) April 2014 – Present

CONFERENCE PRESENTAIONS Epigenetic Pathways to Conduct Problems March 2015 Oral Presentation Society for Research in Child Development, Philadelphia, Pennsylvania, USA

Behavioural and Molecular Changes Associated with Advanced June 2011 Paternal Age: A Mouse Model – Poster Mouse Genetics Conference, Washington, DC, USA

Behavioural and Molecular Changes Associated with Advanced June 2011 Paternal Age: A Mouse Model – Poster Mouse Genetics Conference, Washington, DC, USA

Advancing Paternal Age is Associated with Deficits in Social and March 2011 Exploratory Behaviours in the Offspring – Poster Epigenetics and Developmental Programming Conference, Newcastle upon Tyne

Advancing Paternal Age is Associated with Deficits in Social and October 2010 Exploratory Behaviours in the Offspring – Oral Presentation and Poster 25th IMGC, Crete, Greece

Advancing Paternal Age is Associated with Deficits in Social and September 2010 Exploratory Behaviours in the Offspring – Data Blitz and Poster Parental Brain Conference, University of Edinburgh, Edinburgh

TECHNICAL SKILLS Animal Testing Mouse handling, behavioural tests: holeboard, open field, novel object, social recognition

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Laboratory Techniques PCR, Agarose and SDS-page gel electrophoresis, nucleic acid extraction and purification from blood, cheek swabs and human and animal tissue, DNA sequencing, Taqman genotyping and qPCR, SNaPshot, ABI 3130 genetic analyser sequencing, Sequenom EpiTyper, Sequenom iPlex, Nimblegen microarrays, Affymetrix microarrays, cryosectioning, paraffin sectioning, bisulfite treatment, cell culture, Illumina HiScan Infinium HumanMethylation450 BeadChips, Qiagen pyrosequening, HumanCore genotyping BeadChips and Illumina MiSeq.

Analysis and Computer skills R statistical environment, HTML web design,

REFERENCES Prof Jonathan Mill Dr Katie Lunnon MRC Social, Genetic and Developmental University of Exeter Medical School Psychiatry Centre, Medical Research - Level 4, RILD Building Institute of Psychiatry, Psychology and RD&E Hospital Neuroscience Wonford Kings College London SE5 8AF [email protected] [email protected]