J S YOON, H J LEE and others Treatment of GO by quercetin, 216:2 145–156 Research an antioxidant Cigarette smoke extract-induced adipogenesis in Graves’ orbital fibroblasts is inhibited by quercetin via reduction in oxidative stress Jin Sook Yoon*, Hyun Jung Lee1,*, Min Kyung Chae, Sang Yeul Lee and Eun Jig Lee1,2,† Departments of Ophthalmology, Institute of Vision Research, Yonsei University College of Medicine, Seoul, Korea 1Endocrinology, Brain Korea 21 Project for Medical Science, Institute of Endocrine Research, and Severance Integrative Research Institute for Cerebral and Cardiovascular Disease, Seoul, Korea 2Biochemistry and Molecular Biology, Yonsei University College of Medicine, Seoul, Korea Correspondence *(J S Yoon and H J Lee contributed equally to this work) should be addressed to E J Lee †(E J Lee who is now at Department of Endocrinology, Yonsei University College of Medicine, 50 Yonsei-Ro, Email Seodaemun-Gu, Seoul 120-752, Korea)
[email protected] Abstract Cigarette smoking is known to aggravate Graves’ orbitopathy (GO) severity by enhancing Key Words adipogenesis. We investigated the effect of quercetin, an antioxidant, on adipocyte " quercetin differentiation induced by cigarette smoke extract (CSE) in primary cultured orbital " Graves’ orbitopathy fibroblasts (OFs) from GO patients. Freshly prepared CSE was added to the cells and H2O2 was " heme oxygenase-1 used as a positive control. Intracellular reactive oxygen species (ROS) generation and " cigarette smoke extract Journal of Endocrinology adipogenesis were measured. The expressions of proteins peroxisome proliferator-activated " orbital fibroblasts receptor (PPAR) g, CCAAT-enhancer-binding proteins (C/EBP) a and b, and heme oxygenase-1 " adipogenesis (HO-1), an antioxidant enzyme, were examined during adipogenic differentiation.