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Solutions for Plant Disease Diagnostics LOEWE® Solutions for Plant Disease Diagnostics Catalog 2017 Molecular Diagnostics Rapid Tests ELISA Products Immuno Fluorescence Dear Partners and Colleagues, we invite you to browse our new Catalog for Plant Disease Diagnostics for the year 2017. Although ELISA tests still constitute the majority of our products, our objective is to present to you a choice of high-quality diagnostic tools, that will satisfy your specific needs for pathogen testing. These tools include: • A range of more almost 150 ELISA antisera for the detection of plant viruses, bacteria, and fungi • PCR complete reaction kits and RNA PCR complete reaction kits for easy and convenient molecular detection of plant bacteria and RNA-Viruses • Antisera and supplements for Immuno Fluorescence assays • LOEWE®FAST rapid tests for fast and easy assessment of suspicious plant material Our focus remains on the expansion of our range of molecular diagnostic kits for PCR detection of bacteria, phytoplasms, fungi, and RNA-Viruses. Thanks to our busy R&D team, this product line now already encompasses close to 30 pathogens. NEW: As an additional service for you, we are now also providing kits for easy extraction of high quality DNA and RNA, which are provided by our German partner Analytic Jena AG. A detailed description of our molecular diagnostics can be found on pages 3 and 4. Contact and Order Information Direct and immediate contact to our customers is one of our main goals. Our products can be ordered directly from LOEWE® Biochemica GmbH, Sauerlach, Germany. For ordering please contact us by e-mail to [email protected] or register with our web-shop at: www.loewe-info.com In addition our products are available through a worldwide net of authorized distributors. Please visit our website: www.loewe-info.com/delivery.html for ordering information in your region. The team of LOEWE® Biochemica is looking forward to help you with any questions and inquiries! Sincerely, Dr. Caroline Freye-Minks and Dr. Renate Loewe LOEWE® Mühlweg 2a 82054 Sauerlach Germany Phone: (+49) 8104 61620 Fax: : (+49) 8104 61648 E-Mail: [email protected] LOEWE® Content Product Information Page 3 - 5 NEW: Molecular Extraction Kits Page 6 One-Tube One-Step RNA PCR - Viruses Page 6 - 7 DNA PCR Products Page 8 - 10 DNA PCR Products – Phytoplasma Page 8 DNA PCR Products – Bacteria Page 9 - 10 DNA PCR Products – Fungi Page 10 LOEWE®FAST Rapid Tests Page 11 - 13 ELISA Products Page 14 - 55 ELISA Products – Viruses Page 14 - 47 ELISA Products– Bacteria Page 48 - 54 ELISA Products - Fungi Page 54 - 55 Immuno Fluorescence Products Page 55 - 57 Immuno Fluorescence - Antisera Page 55 - 57 Immuno Fluorescence - Secondary Antibodies Page 57 Immuno Fluorescence - Disposables Page 57 ELISA Chemicals Page 57 - 58 ELISA Disposables Page 58 AGB - General Terms for Sales and Delivery Appendix ® LOEWE Product Information One-Tube One-Step RNA PCR - Viruses The PCR technique allows specific and sensitive detection of plant pathogens, when serological reagents are not available or a second method is needed for confirmation of test results. We are offering a continously growing range of RNA PCR complete reaction kits for reverse transcriptase PCR of RNA plant viruses. Kits Content: All components have been optimized regarding concentrations Premix (Primers, dNTPs) and reaction conditions for reliable and repeatable test results DNA-Polymerase and are accompanied by a comprehensive test protocol. Reverse Transcriptase (M-MLV, RNase H-) The reagents are mixed in one tube on the bench top and the Reaction Buffer reverse transpcription and cDNA amplification take place in one PCR-grade Water reaction step without additional pipetting step, minimalizing the Dithiothreitol (DTT) risk of cross contamination. Positive Control (DNA based) Enzymes are thermostable and kits can be shipped at room Negative Control (DNA based) temperature without the need of dry-ice! Procedure: • extract virus RNA from your sample with your favorite method or our new extraction kits (see below) • run the reverse transcription and cDNA amplification in one tube and one step • run an agarose gel and analyse results Virus-RNA from infected plant 1. Reverse Transcription 2. cDNA Amplification 3 Hours Amplified cDNA Detection in agarose gel NEW: Molecular Extraction Kits * DNA Extraction Kit for Plants RNA Extraction Kit for Plants Kits are specially optimized for the extraction Kits are specially optimized for the extraction and purification of DNA from plant material and purification of RNA from plant material (leaves, stems, roots, flowers). The process (leaves, stems, roots, flowers). Due to its involves a prefiltering step, which effectively chemistry, lysis is highly efficient and two lysis removes non-lysed plant material. Subsequent buffers are included in the kit, in order to enable binding with a novel binding buffer to a spin- processing of a wide range of different plant filter column and elution to a tube enables fast matrices. Within only 30 minutes, total RNA of and easy purification of DNA, which can be used excellent quality can be extracted, which can be directly in downstream applications or stored used directly in downstream applications. One for later use. One kit is sufficient for 50 DNA kit is sufficient for 50 RNA extractions and extractions and contains: contains: lysis buffers 2 different lysis buffers precipitation buffer washing solutions binding and washing solution, proteinase K RNase-free water prefilters, spin filters with receiver and spin filters with receiver and elution tubes elution tubes and manual manual 3 * Kits and descriptions provided by our German partner Analytik Jena AG ® LOEWE Product Information DNA PCR Products Our DNA PCR kits and sets for the detection of bacteria, fungi, and phytoplasms are the perfect tool to establish PCR analysis in your lab! Your sample DNA is subjected to a pre-tested and evaluated PCR system, without the hassle of optimizing reaction- and cycle conditions. DNA PCR Sets The PCR sets contain a premix, including pathogen specific primers and dNTPs for 100 PCR reactions and positive and negative controls for 10 reactions. All components are pre-tested and each product is accompanied by a lot-specific certificate and a working protocol. Complete PCR Reaction Kits DNA PCR Complete Reaction Kits Premix (Primers, dNTPs) Included are a vaildated premix with pathogen specific primers DNA-Polymerase and dNTPs, DNA-based positive and negative controls, 10 x Reaction Buffer thermostable DNA-Polymerase and a detailed protocol. The kits PCR-grade Water can be shipped at room temperature. Positive Control (DNA based) Negative Control (DNA based) LOEWE®FAST Rapid Tests The LOEWE®FAST rapid test series is designed for reliable and specific detection of plant pathogens within minutes. You will be able to assess suspicious plant material in the field or greenhouse without the need of an analytical laboratory. Available for a series of viruses and bacteria like the quarantine pests C.m.s and Ralstonia solanacearum. Fast - easy - on the spot Specific detection of plant pathogens within minutes No laboratory needed Visit our A LOEWE®FAST basic kit includes: You-Tube • 5 single-use test cassettes Channel for a ‘How-to- • 5 pre-filled tubes with sample buffer do Video’ • 5 single-use pipettes Immuno Fluorescence Products Especially if testing a large number of samples, immuno fluorescence still is a very popular and straightforward technique. We are offering a wide range of polyclonal antisera against plant bacteria from goat and rabbit, secondary fluorescence labelled antibodies, as well as multiwell slides and cover slides for immuno fluorescence studies: Antisera and Controls Fluorescence labelled Secondary Antibodies (FITC or Cy3TM) Multiwell Slides Cover Slides 4 ® LOEWE Product Information ELISA Products ELISA (Enzyme-linked Immunosorbent Assay) is the most common test method for the detection of plant pathogens, like viruses and bacteria. LOEWE® offers a range of 150 high quality ELISA products for specific and sensitive detection. ELISA reagents can be supplied as sets or complete kits. All necessary reagents, buffers and plates are also available separately (see pages 57-58, ELISA Chemicals and Disposables). ELISA Sets contain Coating-IgG and AP-Conjugate and are supplied as 100, 300, 500, and for many pathogens as 1000 and 5000 test units. One test corresponds to one well in the ELISA plate. The reagents are optimized and evaluated for a test volume of 200 µl, if not indicated otherwise in the test manual, ensuring highest accuracy, repeatability and sensitivity. ELISA Complete Kits are available for 96, 480, and for many pathogens as 960 test kits. The kits contain all ELISA reagents, positive and negative controls, buffers and ELISA plates or modules to perform the ELISA assay. Components 96 test kits 480 test kits 960 test kits Antibody (IgG) 0.1 ml 0.5 ml 1.0 ml Antibody-AP-conjugate 0.1 ml 0.5 ml 1.0 ml Positive Control 10 tests 10 tests 20 tests Negative Control 10 tests 10 tests 20 tests Coating Buffer 1 liter 1 liter 1 liter Wash Buffer 1 x 5 liter 1 x 5 liter 2 x 5 liter Conjugate/Sample Buffer 1 x 1 liter 2 x 1 liter 4 x 1 liter Substrate Buffer (5x) 1 x 25 ml 1 x 25 ml 2 x 25 ml Substrate Tablets 4 x 5 mg 5 x 20 mg 10 x 20 mg Tween 20 10 ml 10 ml 10 ml High-binding ELISA plates 12 Modules à 8 wells 5 plates 10 plates Sealing Cover 1 sheet 5 sheets 10 sheets ELISA Positive and Negative Controls Negative controls are made from a healthy host plant and are tested for the absence of the respective pathogen. They are lyophilized and sufficient for 10 test wells. Positive controls are made from infected plant material or bacterial cultures, if not stated otherwise. They are lyophilized and sufficient for 10 test wells.
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