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Proceedings of the United States National Museum
Proceedings of the United States National Museum SMITHSONIAN INSTITUTION • WASHINGTON, D.C. Volume 111 1960 Number 3429 A REVISION OF THE GENUS OGCODES LATREILLE WITH PARTICULAR REFERENCE TO SPECIES OF THE WESTERN HEMISPHERE By Evert I. Schlinger^ Introduction The cosmopolitan Ogcodes is the largest genus of the acrocerid or spider-parasite family. As the most highly evolved member of the subfamily Acrocerinae, I place it in the same general line of develop- ment as Holops Philippi, Villalus Cole, Thersitomyia Hunter, and a new South African genus.^ Ogcodes is most closely associated with the latter two genera . The Ogcodes species have never been treated from a world point of view, and this probably accounts for the considerable confusion that exists in the literature. However, several large regional works have been published that were found useful: Cole (1919, Nearctic), Brunetti (192G, miscellaneous species of the world, mostly from Africa and Australia), Pleske (1930, Palaearctic), Sack (1936. Palaearctic), and Sabrosky (1944, 1948, Nearctic). Up to this time 97 specific names have been applied to species and subspecies of this genus. Of these, 19 were considered synonyms, hence 78 species were assumed valid. With the description of 14 new species and the addition of one new name while finding onl}^ five new synonyms, 1 Department of Biological Control, University of California, Riverside, Calif. • This new genus, along with other new species and genera, is being described in forthcoming papers by the author. 227 228 PROCEEDINGS OF THE NATIONAL MUSEUM vol. m we find there are now 88 world species and subspecies. Thus, the total number of known forms is increased by 16 percent. -
Small Wonder Acetone Vaporizer
Acetone Vaporizers and Clearing Supplies: 84-85 Microscope Slides & Cover Glass: 86-87 Sample Collection and Storage Bags: 88 Respirator Fit Test Kits: 89-91 Small Wonder Acetone Vaporizer The Wonder Makers Small Wonder Acetone Vaporizer was developed as an economical and quick way to clear asbestos PCM slides. Specifically designed to meet NIOSH 7400, 7402, or ORM requirements for preparation of PCM samples. Features: ■ Heat control and thermometer built into vaporizer unit ■ Padded carrying case protects equipment and supplies ■ Detailed instruction manual ■ Enough supplies for the analysis of 1/2 gross of slides ■ Lightweight but solidly built ■ Designed in strict compliance with the NIOSH 7400, 7402, or ORM requirements for preparation of microscope slides for PCM analysis Specifications 4-7/8” L x 4-1/2” W x 2-3/4” H (Small Wonder vaporizer) DIMENSIONS 13” L x 11” W x 4” H (Complete Analysis Kit) Plastic case, ABS plastic cover on vaporizer, clear MATERIALS plastic bottles, metal and plastic tools. 2-1/2 lbs. (Small Wonder vaporizer) WEIGHT 8 lbs. (Complete Analysis Kit) Black vaporizer cover with FINISH gray cord. Case color may vary. Complete Kit Shown Includes everything needed to clear slides for PCM analysis PRODUCT DESCRIPTION Small Wonder Analysis Kit WM-42 Includes acetone vaporizer, 1 box microscope slides, 1/2oz Triacetin, 2oz Acetone, 1 microdropper, 1 syringe, 1 scalpel, tweezers, padded carrying case and instruction manual. WM-44 Acetone reagent, 2oz, 99% pure WM-45 Triacetin reagent, 1/2oz, 99% pure WM-47 1cc Syringe WM-48 Rounded Edge Scalpel WM-49 Microdropper 81 The Professionals Choice for Air Sampling Equipment Perm-O-Fix Acetone Vaporizer A simple, easy to use, actone vaporizer for use in preparing asbestos PCM samples as per NIOSH 7400. -
Using Microscopes
Using Microscopes Grade Level: 6, 7, 8, 9, 10 Duration: 30-45 minutes Classification: Classroom Subject(s): Biology, Microbiology Categories (STEM): Science, Technology Keywords: Staining, Microscopes, Microbes Introduction ● Summary: Students will learn how to properly use microscopes and view different cells and organisms. ● Description: Microscopes are often used to view different types of organisms, cells, and organelles. Microscopes can be used to visualize changes in cell structure and identify unknown organisms. Students will learn all the parts of microscopes and how to use and adjust them. Another important step of using microscopes is to learn how to properly prepare slides and wet mounts. Different organisms, cells, and organelles will be viewed under a microscope. In case of school policy limitations, note that student role models are required to cut vegetables at schools before the visit starts. *Note - School is responsible for providing microscopes for this activity. Online Resources: https://www.youtube.com/watch?v=SUo2fHZaZCU\ Vocabulary Organisms: an individual animal, plant, or single-celled life form Cell: smallest structural unit of an organism Organelle: organized structures (compartments) within a living cell Nucleus: dense organelle (generally in the middle of a cell) that contains genetic information Wet Mount: microscope slide holding a specimen suspended in a drop of liquid Materials Materials Quantity Reusable? Nail Polish 1 red per classroom Yes Microscopes (provided by school) TBD by school Yes Dirty -
Microscope Slide-Making Kit
Microscope Slide-Making Kit 665 Carbon Street, Billings, MT 59102 Phone: 800.860.6272 Fax: 888.860.2344 www.homesciencetools.com Copyright 2005 by Home Training Tools, Ltd. All rights reserved. Viewing Slides Scan a slide at low power (usually 40X) to get an overview of the specimen. Center the part of the specimen you want to view at higher power. Adjust your lighting until the slide specimen has clear, sharp contrast. Then switch to medium power (usually 100X) and refocus to observe tissue and cell variations. Repeat at high power (usually 400X). Making Your Own Slides Whole Mounts: Whole mounts are made by placing small objects or specimens whole on a blank slide and then covering them with a coverslip. If your specimen is thick, a concavity slide will work better. You can make whole mounts of many things. Here are some ideas: Colored thread Feather (piece) Thin plant leaf Print (letters) Cloth fibers Hair strand Small insects Dust Algae Pond water Thin paper Insect parts Whole mount specimens must be thin enough to allow light to pass through them. Do not use large, hard objects like rocks, as they can break your slides and microscope lenses. Sections: Section mounts are made by slicing a very thin section of specimen. A cross section is made by slicing across the width or diameter of the specimen. A longitudinal section is made by slicing across the length of the specimen. It is difficult to make sections thin enough without an instrument called a microtome. You can make a simple microtome with a thread spool, a 1/4” diameter bolt at least 2” long, and a 1/4” nut. -
An Antique Microscope Slide Brings the Thrill of Discovery Into a Contemporary Biology Classroom
ARTICLE An Antique Microscope Slide Brings the Thrill of Discovery into a Contemporary Biology Classroom FRANK REISER ABSTRACT that few others share my interests), I add them to my growing collection. The discovery of a Victorian-era microscope slide titled “Grouped Flower Seeds” began When I found skillfully prepared Victorian-era microscope slides listed an investigation into the scientific and historical background of the antique slide to under the category “Folk Art” in an antique dealer’s online catalog, it led develop its usefulness as a multidisciplinary tool for PowerPoint presentations usable in not only to my acquiring them but also to the beginning of a journey contemporary biology classrooms, particularly large-enrollment sections. The resultant involving Internet and library research, trips to herbaria, field collecting presentation was intended to engage students in discussing historical and contempo- excursions, and, finally, to the development of a PowerPoint lecture for rary biology education, as well as some of the intricacies of seed biology. Comparisons between the usefulness and scope of various seed identification resources, both online community college biology students. The slide that triggered the snow- and in print, were made. balling project is titled “Grouped Flower Seeds” (Figure 1). Having spent most of my life as a biology educator, I view most of Key Words: Grouped Flower Seeds; Watson and Sons; antique microscope slide; what I encounter in life from a science-oriented perspective. Finding pre- online seed identification resources; seed identification manuals; seed biology; pared microscope slides described as “folk art” sharply conflicted with large-enrollment lecture. my sense of the correct order of disciplines – particularly for items that I would reverently classify as the tools of scientists. -
Microscopic Exam of Urine Sediment 1
Name:___________________________ Seat # ______ Do not write in pencil (use pen). Do not use Liquid Paper (draw one line through error). Date: ____/____/____ Periods_______________ Please staple all work. Routine Urinalysis with Microscopic Lab Title 4 Lab Number Purpose: The purpose of this lab is to learn how to identify sediment found in urine and learn the clinical significance of urine sediment. Materials: 1. cup to collect urine sample 6. (2) coverslips 11. Laboratory Coat 2. centrifuge 7. test tube rack 12. Disinfecting wipes 3. plastic pipette 8. Microscope 13. Biohazard containers 4. centrifuge tube 9. Sedi-stain 14. Refactometer 5. (1) microscope slide 10. Working Mat (paper towel) 15. Beaker of water Procedure(s): See student handout and pictures to help aid in your procedure. Microscopic Exam of Urine Sediment 1. Collect a fresh urine sample. Obtain a centrifuge tube and put your seat number at the very top of tube. 2. Pour or pipette 12 ml of urine into the centrifuge tube. 3. Centrifuge tube for 5 minutes. Centrifuge Procedure: a. After pouring 12 ml of urine in tube, put in centrifuge and balance your tube with 12 ml of fluid in the same type of tube (pair up with another student). b. Position tubes directly across from each other. c. The teacher will demonstrate how to close and lock lid, and set timer and speed. d. Centrifuge will stop automatically. e. Make sure the centrifuge comes to a complete stop before opening. (Remember the movie OutBreak) ***While your urine is spinning, review how to use the microscope properly (see lesson in lab manual). -
Ideal Conditions for Urine Sample Handling, and Potential in Vitro Artifacts Associated with Urine Storage
Urinalysis Made Easy: The Complete Urinalysis with Images from a Fully Automated Analyzer A. Rick Alleman, DVM, PhD, DABVP, DACVP Lighthouse Veterinary Consultants, LLC Gainesville, FL Ideal conditions for urine sample handling, and potential in vitro artifacts associated with urine storage 1) Potential artifacts associated with refrigeration: a) In vitro crystal formation (especially, calcium oxalate dihydrate) that increases with the duration of storage i) When clinically significant crystalluria is suspected, it is best to confirm the finding with a freshly collected urine sample that has not been refrigerated and which is analyzed within 60 minutes of collection b) A cold urine sample may inhibit enzymatic reactions in the dipstick (e.g. glucose), leading to falsely decreased results. c) The specific gravity of cold urine may be falsely increased, because cold urine is denser than room temperature urine. 2) Potential artifacts associated with prolonged storage at room temperature, and their effects: a) Bacterial overgrowth can cause: i) Increased urine turbidity ii) Altered pH (1) Increased pH, if urease-producing bacteria are present (2) Decreased pH, if bacteria use glucose to form acidic metabolites iii) Decreased concentration of chemicals that may be metabolized by bacteria (e.g. glucose, ketones) iv) Increased number of bacteria in urine sediment v) Altered urine culture results b) Increased urine pH, which may occur due to loss of carbon dioxide or bacterial overgrowth, can cause: i) False positive dipstick protein reaction ii) Degeneration of cells and casts iii) Alter the type and amount of crystals present 3) Other potential artifacts: a) Evaporative loss of volatile substances (e.g. -
Microbial Discovery Activity Pond Scum – Page 2
MMMiiicccrrrooobbbiiiaaalll DDDiiissscccooovvveeerrryyy AAAccctttiiivvviiitttyyy PPPooonnnddd SSScccuuummm::: IIInnnvvveeessstttiiigggaaatttiiinnnggg MMMiiicccrrroooooogggrraaannniiisssmmmsss Author Mark Gallo, Ph.D. Associate Professor of Biology Niagara University, NY 14109 [email protected] Contributor George A. Jacob, Ph.D. St. Xavier High School Cincinnati, OH 45224 [email protected] Intended Audience K-4 X 5-8 X 9-12 X Activity Specifications Classroom setting X Requires special equipment X Uses hands-on manipulatives X Requires mathematical skills Can be performed individually X Requires group work Requires more than one (45 min) class period X Appropriate for special needs student X American Society for Microbiology Education Department 1752 N Street, NW Washington, DC 20036 [email protected] IIInnntttrrroooddduuuccctttiiiooonnn Description In this exercise, students discover a diversity of microorganisms living in a small drop of pond water. Abstract Little else can stimulate a student’s interest in biology like a drop of pond water teaming with invisible life viewed with a microscope. This activity describes two means of observing pond water other than the traditional hanging drop or temporary wet mount slide preparation. Core Themes Addressed Microorganisms and the Environment Keywords Aquatic, Environment, Protozoa, Biofilm, Water quality, Classification Learning Objectives At completion of this activity, learner will be able to: • appreciate (acknowledge) the previously unseen life in pond water. • prepare a biofilm slide. • draw and describe a microorganism. National Science Education Standards Addressed Unifying Concepts and Processes – This activity incorporates evidence of microscopic life. Standard 1: Science as Inquiry – In completion of this activity student performs directed, inquiry based observations of microorganisms. Standard 3: Life science - In completion of this activity student will have had a chance to view living organisms from an environment and determine the characteristics of the microorganisms. -
Arthropod Pests
IAEA-TECDOC-1082 XA9950282--W6 Irradiationa as quarantine treatmentof arthropod pests Proceedings finala of Research Co-ordination Meeting organizedthe by Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture and held Honolulu,in Hawaii, November3-7 1997 INTERNATIONAL ATOMIC ENERGY AGENCY /A> 30- 22 199y Ma 9 J> The originating Section of this publication in the IAEA was: Food and Environmental Protection Section International Atomic Energy Agency Wagramer Strasse 5 0 10 x Bo P.O. A-1400 Vienna, Austria The IAEA does not normally maintain stocks of reports in this series However, copies of these reports on microfiche or in electronic form can be obtained from IMS Clearinghouse International Atomic Energy Agency Wagramer Strasse5 P.O.Box 100 A-1400 Vienna, Austria E-mail: CHOUSE® IAEA.ORG URL: http //www laea org/programmes/mis/inis.htm Orders shoul accompaniee db prepaymeny db f Austriao t n Schillings 100,- in the form of a cheque or in the form of IAEA microfiche service coupons which may be ordered separately from the INIS Clearinghouse IRRADIATIO QUARANTINA S NA E TREATMENF TO ARTHROPOD PESTS IAEA, VIENNA, 1999 IAEA-TECDOC-1082 ISSN 1011-4289 ©IAEA, 1999 Printe IAEe th AustriAn y i d b a May 1999 FOREWORD Fresh horticultural produce from tropical and sub-tropical areas often harbours insects and mites and are quarantined by importing countries. Such commodities cannot gain access to countries which have strict quarantine regulations suc Australias ha , Japan Zealanw Ne , d e Uniteth d dan State f Americo s a unless treaten approvea y b d d method/proceduro t e eliminate such pests. -
EJC Cover Page
Early Journal Content on JSTOR, Free to Anyone in the World This article is one of nearly 500,000 scholarly works digitized and made freely available to everyone in the world by JSTOR. Known as the Early Journal Content, this set of works include research articles, news, letters, and other writings published in more than 200 of the oldest leading academic journals. The works date from the mid-seventeenth to the early twentieth centuries. We encourage people to read and share the Early Journal Content openly and to tell others that this resource exists. People may post this content online or redistribute in any way for non-commercial purposes. Read more about Early Journal Content at http://about.jstor.org/participate-jstor/individuals/early- journal-content. JSTOR is a digital library of academic journals, books, and primary source objects. JSTOR helps people discover, use, and build upon a wide range of content through a powerful research and teaching platform, and preserves this content for future generations. JSTOR is part of ITHAKA, a not-for-profit organization that also includes Ithaka S+R and Portico. For more information about JSTOR, please contact [email protected]. TRANSACTIONS OF THE AMERICANENTOMOLOGICAL SOCIETY VOLUME XLV THE DIPTEROUS FAMILY CYRTIDAE IN NORTH AMERICA BY F. R. COLE U. S. Bureau of Entomology1 Introduction This paper is the result of about two years interrupted study of the dipterous family Cyrtidae. It is an interesting little group of insects with a remarkable range of variation in structure. The collecting of more material will no doubt cause some changes to be made in the status of a few species, and further study will reveal other characters for the separation of the different forms. -
Innovative Methods of Archiving, Presentation and Providing Access to Histological Sections
ADVANCES IN CELL BIOLOGY VOL. 3, ISSUE 3/2011 (41–54) INNOVATIVE METHODS OF ARCHIVING, PRESENTATION AND PROVIDING ACCESS TO HISTOLOGICAL SECTIONS Krystyna FILIPIAK, Agnieszka MALIŃSKA, Dariusz KRUPA, Maciej ZABEL Department of Histology and Embryology, Poznań University of Medical Sciences, Poland DOI: 10.2478/v10052-011-0003-4 Summary: The dynamic development of technical sciences and informatics makes now possible acquisition of microscopic images of histological sections, not only using digital cameras, but also through specialized devices called scanners. The digitalized images stored in a computer storage device are called virtual slides and, together with special software, are known as virtual microscopy. The virtual slides can be analyzed on a computer screen by panoramic viewing or using a detailed image examination at higher magnification. In many research and education institutions in both the U.S. and Europe, the virtual microscopy is used for teaching and training purposes. In the academic year of 2009/10, Department of Histology and Embryology, University of Medical Sciences in Poznan, as one of the first in Poland, has created a virtual database for educational purposes. This database created by archiving the traditional images of histological slides in the form of digital images. So far, more than 130 virtual slides have been acquired and catalogued in 24 thematic folders, available for medical students participating in histology, embryology and cell biology courses. Telepathology is the second branch which uses virtual microscopy. Virtual microscope allows to discuss and resolve medical/diagnostic problems with the use of telecommunication systems and information technology. The existing internet platforms offer access to virtual microscopes and virtual slides. -
STAINING TECHNIQUES Staining Is an Auxiliary Technique Used in Microscopy to Enhance Contrast in the Microscopic Image
STAINING TECHNIQUES Staining is an auxiliary technique used in microscopy to enhance contrast in the microscopic image. Stains or dyes are used in biology and medicine to highlight structures in biological tissues for viewing with microscope. Cell staining is a technique that can be used to better visualize cells and cell components under a microscope. Using different stains, it is possible to stain preferentially certain cell components, such as a nucleus or a cell wall, or the entire cell. Most stains can be used on fixed, or non-living cells, while only some can be used on living cells; some stains can be used on either living or non-living cells. In biochemistry, staining involves adding a class specific (DNA, lipids, proteins or carbohydrates) dye to a substrate to qualify or quantify the presence of a specific compound. Staining and fluorescence tagging can serve similar purposes Purposes of Staining The most basic reason that cells are stained is to enhance visualization of the cell or certain cellular components under a microscope. Cells may also be stained to highlight metabolic processes or to differentiate between live and dead cells in a sample. Cells may also be enumerated by staining cells to determine biomass in an environment of interest. Stains may be used to define and examine bulk tissues (e.g. muscle fibers or connective tissues), cell populations (different blood cells) or organelles within individual cells. Biological staining is also used to mark cells in flow cytometry, flag proteins or nucleic acids on gel electrophoresis Staining is not limited to biological materials, it can also be used to study the morphology (form) of other materials e.g.