bioRxiv preprint doi: https://doi.org/10.1101/553123; this version posted February 18, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Does Long-Term Selection for Development Time Result in Canalization: A Test Using Drosophila melanogaster * * Shampa M. Ghosh1,2 , K. M. Satish1,3, J. Mohan1,4 and Amitabh Joshi1 Author affiliations 1: Evolutionary Biology Laboratory, Evolutionary & Organismal Biology Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur, Bengaluru 560064, India 2: Present address: Fly Research Laboratory, School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT) Campus 11, Bhubaneswar 751024, India 3: Present address: Department of Biotechnology, College of Agriculture, Navile, University of Agricultural and Horticultural Sciences, Shivamogga 577201, India 4: Present address: Centre of Excellence in Genomics and Translational Medicine, University of Tartu, Tartu 50411, Estonia *Correspondence: Dr. Shampa M. Ghosh:
[email protected] Dr. Amitabh Joshi:
[email protected] Abstract Canalization denotes the robustness of a trait against genetic or environmental perturbation. Plasticity, in contrast indicates the environmental sensitivity of a trait. Stabilizing selection is thought to increase canalization of a trait, whereas directional selection is often thought to lead to decanalization. However, the relationship between selection, canalization and plasticity remains largely unclear. Experimental evolution is a powerful approach for addressing fundamental questions in evolution. Here, we ask whether long-term directional selection for reduced pre-adult development time in Drosophila melanogaster results in the evolution of increased canalization for development time, the trait under primary selection.