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Commonwealth of Massachusetts Interdepartmental Service Agreement ISAEQE22309702ENV15A

Wetlands Monitoring and Assessment Salt Marsh Data Collection and Processing Report 2015-2016 Northeast Basin Group Parker and Ipswich Basins

December 30, 2016

Prepared for: Massachusetts Department of Environmental Protection Wetlands Program 1 Winter Street Boston, MA 02108

Prepared by: Massachusetts Office of Coastal Zone Management 251 Causeway Street, Suite 800 Boston, MA 02114

This report describes work performed under an Interagency Service Agreement (ISA) (ISAEQE22309702ENV15A) by the Massachusetts Office of Coastal Zone Management (CZM), for the Massachusetts Department of Environmental Protection (DEP) from 2015-2016.

CZM was contracted to collect data on vegetation and macroinvertebrates at twenty salt marsh sites in the DEP Surface Water Monitoring Program’s Northeast Basin Group in 2015—specifically, the Parker and Ipswich basins. Work was extended to 2016 due to unforeseen circumstances (e.g., one key staff member on maternity leave, and another with a knee injury preventing fieldwork). All data were collected following a Quality Assurance Project Plan (QAPP) previously approved by DEP and the U.S. Environmental Protection Agency (EPA).

Sampling Design CZM staff selected sites using a stratified random design in coordination with DEP staff. Index of Ecological Integrity (IEI) values from the Conservation Assessment and Prioritization System (CAPS) were averaged for salt marshes within each sub-watershed of the target basins. A random point generator was used to identify over 100 points within the sub-watersheds with the lowest average IEI scores (< 0.5), and 25 points (> 0.8) within those with the highest average IEI values. The random point generator was specified with a 500-meter buffer to allow for geographic variability. Points were manually assessed to ensure that they meet the following sampling criteria, with the goal of identifying 15 sites within the “low IEI” sub-watersheds, and 5 sites within the “high IEI” sub-watersheds (Figure 1).

Sampling Criteria  The point is within 200 meters of a salt marsh creek (each sampling point will be moved not more than 200 m to the nearest tidal creek suitable for sampling);  The creek is suitable for auger and D-Net macroinvertebrate sampling (e.g., channel width is at least 2 m, bank height is at least 1 m, and bank height and/or condition does not compromise safety);  The point is not deemed inaccessible due to physical barriers;  The point is not deemed inaccessible due to legal barriers (i.e., permission to access private property is granted).

Permitting CZM sought and received permission to access and sample vegetation and macroinvertebrates on properties owned by the U.S. Fish and Wildlife Service (USFWS), The Trustees of Reservations (TTOR), Essex County Greenbelt Association, and private commercial or residential landowners (e.g., Perley’s Marina, Rowley). Special Use Permits were required by TTOR and USFWS.

Data Collection and Processing Data were collected following the protocols described in the current standard operating procedures document (SOP), Appendix A of the salt marsh assessment QAPP approved by DEP and EPA. The SOP also includes information on the processing of macroinvertebrate specimens, including sorting, identification, and QA/QC testing.

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Figure 1. Salt marsh site level assessment sites in the North Coastal Basin—specifically the Parker and Ipswich River sub-basins—selected by stratified random sampling design. Fifteen sites were randomly selected within the sub- watersheds with the lowest average IEI scores (< 0.5) for salt marshes. Five sites were randomly selected within the sub-watersheds with the highest average IEI scores > (0.8).

Data were entered into spreadsheets with the same formatting used to report CAPS site-level assessment data to UMass for inclusion in the CAPS project database for development of Indices of Biological Integrity (IBIs). Two spreadsheets are included: caps_saltmarsh_vegetation_2015-2016.xlsx and caps_saltmarsh_macroinvertebrates_2015-2016.xlsx. The vegetation spreadsheet includes worksheets containing information on sampling methods, plot records (GPS locations, dates and times of sampling, etc.), vegetation data, nomenclature changes, collector codes, and a database dictionary / metadata record. The macroinvertebrate spreadsheet includes sample records, macroinvertebrate data, collector codes, an updated bench form (master list of taxa recorded since CAPS project inception), and a database dictionary / metadata record.

Results Summary

Twenty-four vascular plant taxa were recorded along 60 salt marsh transects (three per site), and 65 macroinvertebrate taxa were recorded in 40 dip net samples, 40 auger samples, and 40 quadrat observations (two samples of each type per site).

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YrPlotID PlotID Phylum Class Subclass SubOrder Family Species FinalID A1 A2 D1 D2 Q1 Q2 Notes 150802 8-2 Arthropoda Malacostraca Amphipoda Talitridae Talitridae 0 0 0 0 16 31 150802 8-2 Ellobiidae Melampus bidentatus Melampus bidentatus 0 0 0 0 17 8 150802 8-2 Arthropoda Insecta Hemiptera Auchenorrhyncha Auchenorrhyncha 0 0 0 0 2 1 150802 8-2 Arthropoda Arachnida Araneae Araneae 0 0 0 0 6 1 150802 8-2 Arthropoda Arachnida Acari Acari 0 0 0 0 0 3 150802 8-2 Arthropoda Insecta Diptera Diptera 0 0 0 0 3 0 150802 8-2 Arthropoda (larvae) Insecta (larvae) Diptera (larvae) Diptera (larvae) 0 0 1 1 1 0 150802 8-2 Arthropoda Insecta Hemiptera Auchenorrhyncha Delphacidae Delphacidae 1 0 0 0 0 0 150802 8-2 Arthropoda Malacostraca Tanaidacea Tanaidacea 4 4 82 0 0 0 150802 8-2 Arthropoda Malacostraca Amphipoda Corophiidae Corophiidae 18 22 2 0 0 0 150802 8-2 Arthropoda Malacostraca Eumalacostraca Amphipoda Gammaridea Melitidae Melitidae 5 1 0 0 0 0 150802 8-2 Arthropoda Malacostraca Isopoda Anthuridae Anthuridae 2 0 2 0 0 0 D1: most spionids and 150802 8-2 Annelida Polychaeta Spionida Spionidae 0 1 109 0 0 0 some ampharetidae missing tentacles D1: most spionids and 150802 8-2 Annelida Polychaeta Ampharetidae Ampharetidae 0 1 20 0 0 0 some ampharetidae missing tentacles D1: Sabellidae very small, 150802 8-2 Annelida Polychaeta Sabellida Sabellidae Sabellidae 0 0 4 0 0 0 difficult to remove from tube - in 2 pieces 150802 8-2 Mollusca Gastropoda Hydrobiidae 0 0 4 0 0 0 150802 8-2 Mollusca Gastropoda Littorinimorpha Littorinidae 0 0 3 0 0 0 150802 8-2 Mollusca Myoida Myidae Myidae 0 0 1 0 0 0 150802 8-2 Annelida Clitellata Hirudinea Hirudinea 0 0 10 0 0 0 150802 8-2 Nematoda Nematoda 0 0 7 0 0 0 150802 8-2 Arthropoda Insecta Hymenoptera Hymenoptera 0 0 1 0 0 0 150802 8-2 Arthropoda Insecta Orthoptera Orthoptera 0 0 2 0 0 0 150802 8-2 Arthropoda Malacostraca Cumacea Cumacea 0 0 1 0 0 0 150802 8-2 Arthropoda Malacostraca Amphipoda Gammaridae Gammaridae 0 0 3 0 0 0 150802 8-2 Arthropoda Malacostraca Isopoda Idoteidae Idoteidae 0 0 2 0 0 0 150802 8-2 Arthropoda Malacostraca Decapoda Palaemonidae Palaemonetes pugio Palaemonetes pugio 0 0 2 1 0 0 150802 8-2 Arthropoda Insecta Hemiptera Heteroptera Corixidae Corixidae 0 0 0 1 0 0 150807 8-7 Arthropoda Malacostraca Amphipoda Talitridae Talitridae 0 0 0 0 21 85 150807 8-7 Mollusca Bivalvia Mytiloida demissa 0 0 0 0 6 2 150807 8-7 Mollusca Gastropoda Littorinimorpha Littorinidae saxatilis 0 0 0 0 2 25 150807 8-7 Arthropoda Insecta Hemiptera Auchenorrhyncha Auchenorrhyncha 0 0 0 0 14 43 150807 8-7 Arthropoda Arachnida Araneae Araneae 0 0 0 0 10 21 150807 8-7 Arthropoda Arachnida Acari Acari 0 0 0 0 1 0 150807 8-7 Arthropoda Insecta Diptera Diptera 0 0 0 0 0 2 150807 8-7 Annelida Polychaeta Phyllodocida Nereididae Nereididae 1 0 0 0 0 0 150807 8-7 Annelida Annelida (Capitellidae/Oligochaeta type) 0 2 0 0 0 0 150807 8-7 Annelida Polychaeta Scolecida Orbiniidae Orbiniidae 0 1 0 0 0 0 150807 8-7 Annelida Polychaeta Phyllodocida Phyllodocidae Phyllodocidae 0 3 0 0 0 0 150807 8-7 Mollusca Gastropoda Littorinimorpha Littorinidae Littorinidae 0 0 0 4 0 0 151202 12-2 Arthropoda Malacostraca Amphipoda Talitridae Talitridae 0 0 0 0 5 1 151202 12-2 Mollusca Gastropoda Ellobiidae Melampus bidentatus Melampus bidentatus 0 0 0 0 8 5 151202 12-2 Arthropoda Arachnida Araneae Araneae 0 0 0 1 1 2 151202 12-2 Arthropoda Insecta Coleoptera Coleoptera 0 0 0 0 3 1 151202 12-2 Arthropoda (larvae) Insecta (larvae) Coleoptera (larvae) Coleoptera (larvae) 0 0 0 0 0 1 151202 12-2 Annelida Polychaeta Phyllodocida Nereididae Nereididae 1 0 0 0 0 0 151202 12-2 Annelida Polychaeta Terebellida Ampharetidae Ampharetidae 1 0 0 0 0 0 151202 12-2 Arthropoda Malacostraca Tanaidacea Tanaidacea 1 1 0 0 0 0 151202 12-2 Annelida Annelida (unknown) 0 1 0 0 0 0 A2: Heavily damaged 151202 12-2 Arthropoda Malacostraca Amphipoda Aoridae Aoridae 0 1 0 0 0 0 151202 12-2 Arthropoda Insecta Hemiptera Sternorrhyncha Aphididae Aphididae 0 0 3 0 0 0 151202 12-2 Arthropoda Malacostraca Amphipoda Corophiidae Corophiidae 0 0 0 2 0 0 151202 12-2 Arthropoda Malacostraca Eumalacostraca Amphipoda Gammaridea Melitidae Melitidae 0 0 0 3 0 0 151203 12-3 Arthropoda Malacostraca Amphipoda Talitridae Talitridae 0 0 0 0 2 18 151203 12-3 Mollusca Gastropoda Ellobiidae Melampus bidentatus Melampus bidentatus 0 0 0 0 3 13 151203 12-3 Arthropoda Arachnida Araneae Araneae 0 0 0 0 0 3 151203 12-3 Arthropoda Insecta Hymenoptera Formicidae Formicidae 0 0 0 0 0 1 151203 12-3 Annelida Polychaeta Terebellida Ampharetidae Ampharetidae 0 0 1 0 0 0 151203 12-3 Mollusca Gastropoda Littorinimorpha Hydrobiidae Hydrobiidae 0 0 2 0 0 0 151203 12-3 Annelida Clitellata Hirudinea Hirudinea 0 0 4 2 0 0 151203 12-3 Arthropoda (larvae) Insecta (larvae) Diptera (larvae) Diptera (larvae) 0 0 1 0 0 0 151203 12-3 Arthropoda Malacostraca Amphipoda Gammaridae Gammaridae 0 0 1 0 0 0 151203 12-3 Arthropoda Malacostraca Decapoda Palaemonidae Palaemonetes pugio Palaemonetes pugio 0 0 4 3 0 0 D1: may be molt, bad 151203 12-3 Arthropoda Malacostraca Eumalacostraca Decapoda Pleocyemata Panopeidae Rhithropanopeus harrisii Rhithropanopeus harrisii 0 0 1 0 0 0 shape 151203 12-3 Arthropoda Insecta Diptera Diptera 0 0 0 1 0 0 151203 12-3 Arthropoda Insecta Hemiptera Auchenorrhyncha Cicadellidae Cicadellidae 0 0 0 1 0 0 151204 12-4 Arthropoda Malacostraca Amphipoda Talitridae Talitridae 0 0 1 2 48 15 151204 12-4 Mollusca Gastropoda Ellobiidae Melampus bidentatus Melampus bidentatus 0 0 0 0 41 94 151204 12-4 Arthropoda Insecta Hemiptera Sternorrhyncha Aphididae Aphididae 0 0 0 0 0 1 151204 12-4 Arthropoda Insecta Hemiptera Auchenorrhyncha Auchenorrhyncha 0 0 0 0 2 28 151204 12-4 Arthropoda Arachnida Araneae Araneae 0 0 0 0 1 5 151204 12-4 Arthropoda Insecta Coleoptera Coleoptera 0 0 1 0 0 3 151204 12-4 Arthropoda Malacostraca Tanaidacea Tanaidacea 4 36 0 0 0 0 151204 12-4 Annelida Annelida (Capitellidae/Oligochaeta type) 0 3 0 0 0 0 A2: some spionids 151204 12-4 Annelida Polychaeta Spionida Spionidae Spionidae 0 8 0 0 0 0 damaged without head appendages 151204 12-4 Arthropoda Insecta Hemiptera Hemiptera 0 2 0 0 0 0 151204 12-4 Arthropoda Malacostraca Amphipoda Gammaridae Gammaridae 0 1 0 0 0 0 151204 12-4 Arthropoda (larvae) Insecta (larvae) Diptera (larvae) Diptera (larvae) 0 0 1 0 0 0 151214 12-14 Arthropoda Malacostraca Amphipoda Talitridae Talitridae 0 0 0 0 7 6 151214 12-14 Arthropoda Malacostraca Isopoda Isopoda 0 0 0 0 6 10 151214 12-14 Mollusca Bivalvia Mytiloida Mytilidae Geukensia demissa Geukensia demissa 0 0 0 0 0 4 151214 12-14 Mollusca Gastropoda Ellobiidae Melampus bidentatus Melampus bidentatus 0 0 0 0 65 236 151214 12-14 Arthropoda Insecta Hemiptera Auchenorrhyncha Auchenorrhyncha 0 0 0 0 2 5 151214 12-14 Arthropoda Arachnida Araneae Araneae 0 0 1 1 5 5 151214 12-14 Arthropoda Insecta Diptera Diptera 0 0 0 0 0 1 151214 12-14 Arthropoda Insecta Coleoptera Coleoptera 0 0 0 0 1 0 151214 12-14 Arthropoda Insecta Hymenoptera Formicidae Formicidae 0 0 0 0 1 0 151214 12-14 Annelida Polychaeta Spionida Spionidae Spionidae 2 0 0 0 0 0 151214 12-14 Arthropoda Insecta Hemiptera Auchenorrhyncha Delphacidae Delphacidae 1 0 0 0 0 0 151214 12-14 Arthropoda Malacostraca Tanaidacea Tanaidacea 1 0 0 0 0 0 151214 12-14 Arthropoda (larvae) Insecta (larvae) Pterygota (larvae) Diptera (larvae) Brachycera (larvae) Stratiomyidae Stratiomyidae 0 0 1 0 0 0 151214 12-14 Arthropoda Malacostraca Decapoda Palaemonidae Palaemonetes pugio Palaemonetes pugio 0 0 1 0 0 0 151214 12-14 Arthropoda (larvae) Insecta (larvae) Diptera (larvae) Diptera (larvae) 0 0 0 1 0 0 151214 12-14 Arthropoda Insecta Orthoptera Orthoptera 0 0 0 1 0 0 151214 12-14 Arthropoda Insecta Hemiptera Heteroptera Miridae Miridae 0 0 0 1 0 0 151215 12-15 Arthropoda Malacostraca Amphipoda Talitridae Talitridae 0 0 8 0 1 0 151215 12-15 Mollusca Gastropoda Ellobiidae Melampus bidentatus Melampus bidentatus 0 0 0 0 1 3 151215 12-15 Arthropoda Insecta Hemiptera Auchenorrhyncha Auchenorrhyncha 0 0 0 0 1 1 151215 12-15 Arthropoda Arachnida Araneae Araneae 0 0 6 8 0 2 151215 12-15 Arthropoda Insecta Dictyoptera Mantodea Mantodea 0 0 0 0 1 0 A1: fragments 151215 12-15 Annelida Annelida (Polychaeta) 1 0 0 0 0 0 was only thing in vial 151215 12-15 Arthropoda Malacostraca Eumalacostraca Amphipoda Gammaridea Melitidae Melitidae 0 1 0 0 0 0 151215 12-15 Arthropoda Insecta Diptera Diptera 0 0 3 2 0 0 151215 12-15 Arthropoda Insecta Coleoptera Curculionidae Curculionidae 0 0 1 2 0 0 151215 12-15 Arthropoda Insecta Hemiptera Auchenorrhyncha Delphacidae Delphacidae 0 0 2 0 0 0 151215 12-15 Arthropoda Insecta Hymenoptera Hymenoptera 0 0 0 1 0 0 151218 12-18 Arthropoda Malacostraca Amphipoda Talitridae Talitridae 0 0 1 0 2 8 151218 12-18 Mollusca Bivalvia Mytiloida Mytilidae Geukensia demissa Geukensia demissa 0 0 0 0 2 0 151218 12-18 Mollusca Gastropoda Ellobiidae Melampus bidentatus Melampus bidentatus 0 0 0 0 18 135 151218 12-18 Arthropoda Insecta Hemiptera Auchenorrhyncha Auchenorrhyncha 0 0 0 0 3 1 151218 12-18 Arthropoda Insecta Collembola Collembola 0 0 0 0 1 0 151218 12-18 Arthropoda Arachnida Araneae Araneae 0 0 0 0 0 1 151218 12-18 Arthropoda Insecta Coleoptera Coleoptera 0 0 0 0 2 0 151218 12-18 Annelida Polychaeta Phyllodocida Nereididae Nereididae 2 1 0 0 0 0 151218 12-18 Annelida Polychaeta Spionida Spionidae Spionidae 4 2 0 0 0 0 151218 12-18 Arthropoda Malacostraca Tanaidacea Tanaidacea 1 0 0 0 0 0 151218 12-18 Arthropoda Malacostraca Amphipoda Corophiidae Corophiidae 5 0 0 0 0 0 151218 12-18 Arthropoda Malacostraca Isopoda Anthuridae Anthuridae 2 0 0 0 0 0 151218 12-18 Arthropoda Insecta Hymenoptera Hymenoptera 0 1 0 0 0 0 151218 12-18 Arthropoda Malacostraca Decapoda Palaemonidae Palaemonetes pugio Palaemonetes pugio 0 0 0 1 0 0 151221 12-21 Arthropoda Malacostraca Amphipoda Talitridae Talitridae 0 0 2 16 38 9 151221 12-21 Mollusca Gastropoda Ellobiidae Melampus bidentatus Melampus bidentatus 0 0 0 0 16 17 151221 12-21 Arthropoda Insecta Hemiptera Heteroptera Miridae Miridae 0 0 2 0 1 0 D2: poor shape, no 151221 12-21 Arthropoda Insecta Hemiptera Auchenorrhyncha Auchenorrhyncha 0 0 0 1 10 3 appendages 151221 12-21 Arthropoda Arachnida Araneae Araneae 1 0 1 1 25 7 151221 12-21 Arthropoda Arachnida Acari Acari 0 0 0 0 4 4 151221 12-21 Arthropoda Insecta Diptera Diptera 0 0 3 0 0 2 151221 12-21 Arthropoda Insecta Coleoptera Coleoptera 0 0 0 0 8 2 151221 12-21 Arthropoda Insecta Hymenoptera Formicidae Formicidae 0 0 0 0 1 0 151221 12-21 Annelida Clitellata Oligochaeta Oligochaeta 3 0 0 0 0 0 151221 12-21 Annelida Polychaeta Phyllodocida Nereididae Nereididae 1 2 0 0 0 0 151221 12-21 Arthropoda (larvae) Insecta (larvae) Diptera (larvae) Diptera (larvae) 1 0 2 1 0 0 151221 12-21 Arthropoda Insecta Hemiptera Auchenorrhyncha Delphacidae Delphacidae 1 0 4 3 0 0 151221 12-21 Arthropoda Malacostraca Tanaidacea Tanaidacea 1 0 0 0 0 0 A2: very poor condition; 151221 12-21 Arthropoda Malacostraca Amphipoda Amphipoda 0 1 0 1 0 0 D2: likely Talitridae but no head present 151221 12-21 Arthropoda Malacostraca Amphipoda Gammaridae Gammaridae 0 0 1 0 0 0 151221 12-21 Arthropoda Malacostraca Eumalacostraca Decapoda Pleocyemata Panopeidae Rhithropanopeus harrisii Rhithropanopeus harrisii 0 0 1 0 0 0 151221 12-21 Arthropoda (pupae) Insecta (pupae) Diptera (pupae) Diptera (pupae) 0 0 0 1 0 0 151221 12-21 Arthropoda Insecta Hymenoptera Hymenoptera 0 0 0 2 0 0 151221 12-21 Arthropoda Insecta Coleoptera Curculionidae Curculionidae 0 0 0 1 0 0 151221 12-21 Arthropoda Malacostraca Decapoda Palaemonidae Palaemonetes pugio Palaemonetes pugio 0 0 0 1 0 0 151222 12-22 Arthropoda Malacostraca Amphipoda Talitridae Talitridae 0 0 0 3 10 21 151222 12-22 Arthropoda Malacostraca Isopoda Isopoda 0 0 0 0 6 4 151222 12-22 Mollusca Gastropoda Ellobiidae Melampus bidentatus Melampus bidentatus 0 0 0 0 10 21 151222 12-22 Arthropoda Insecta Hemiptera Auchenorrhyncha Auchenorrhyncha 0 0 0 0 0 4 151222 12-22 Arthropoda Arachnida Araneae Araneae 0 0 3 1 8 14 151222 12-22 Arthropoda Insecta Diptera Diptera 0 0 0 0 2 0 151222 12-22 Arthropoda Insecta Coleoptera Coleoptera 0 0 0 0 0 2 Q2: Ant colony, count on 151222 12-22 Arthropoda Insecta Hymenoptera Formicidae Formicidae 0 0 1 0 50 0 datasheet recorded as 50+ 151222 12-22 Arthropoda (larvae) Insecta (larvae) Diptera (larvae) Diptera (larvae) 1 1 1 0 0 0 151222 12-22 Annelida Polychaeta Terebellida Ampharetidae Ampharetidae 0 2 7 2 0 0 151222 12-22 Arthropoda Malacostraca Tanaidacea Tanaidacea 0 7 0 3 0 0 151222 12-22 Arthropoda Malacostraca Amphipoda Corophiidae Corophiidae 0 1 0 0 0 0 151222 12-22 Cnidaria Anthozoa Actiniaria Edwardsiidae Edwardsiidae 0 1 0 2 0 0 151222 12-22 Mollusca Gastropoda Littorinimorpha Hydrobiidae Hydrobiidae 0 0 6 2 0 0 151222 12-22 Mollusca Gastropoda Littorinimorpha Littorinidae Littorinidae 0 0 5 0 0 0 151222 12-22 Arthropoda Insecta Pterygota Hemiptera Heteroptera Belostomatidae Belostomatidae 0 0 1 0 0 0 151222 12-22 Arthropoda (pupae) Insecta (pupae) Insecta (pupae) 0 0 1 0 0 0 151222 12-22 Arthropoda Maxillopoda Branchiura 0 0 0 3 0 0 151222 12-22 Arthropoda Malacostraca Isopoda Oniscidae Oniscidae 0 0 0 1 0 0 151232 12-32 Arthropoda Malacostraca Amphipoda Talitridae Talitridae 0 0 3 0 0 25 151232 12-32 Arthropoda Malacostraca Isopoda Isopoda 0 0 0 0 11 22 151232 12-32 Mollusca Gastropoda Ellobiidae Melampus bidentatus Melampus bidentatus 0 0 0 0 151 104 151232 12-32 Arthropoda Insecta Hemiptera Auchenorrhyncha Auchenorrhyncha 0 0 0 0 3 0 151232 12-32 Arthropoda Arachnida Acari Acari 0 0 0 0 0 1 151232 12-32 Arthropoda Insecta Coleoptera Coleoptera 0 0 0 0 1 0 151232 12-32 Annelida Polychaeta Phyllodocida Nereididae Nereididae 1 0 0 0 0 0 151232 12-32 Arthropoda Malacostraca Isopoda Anthuridae Anthuridae 1 0 0 0 0 0 151232 12-32 Annelida Annelida (Capitellidae/Oligochaeta type) 0 0 1 0 0 0 151232 12-32 Arthropoda (larvae) Insecta (larvae) Diptera (larvae) Diptera (larvae) 0 0 1 0 0 0 151232 12-32 Arthropoda Malacostraca Tanaidacea Tanaidacea 0 0 9 0 0 0 151235 12-35 Arthropoda Malacostraca Amphipoda Talitridae Talitridae 0 0 1 0 14 22 151235 12-35 Mollusca Gastropoda Ellobiidae Melampus bidentatus Melampus bidentatus 0 0 0 0 24 45 151235 12-35 Arthropoda Insecta Hemiptera Auchenorrhyncha Auchenorrhyncha 0 0 0 0 2 0 D2: egg sack w/multiple 151235 12-35 Arthropoda Arachnida Araneae Araneae 0 0 2 1 5 2 spiders inside only counted the one egg sack 151235 12-35 Annelida Polychaeta Phyllodocida Nereididae Nereididae 3 0 0 0 0 0 151235 12-35 Annelida Polychaeta Spionida Spionidae Spionidae 1 0 0 0 0 0 151235 12-35 Arthropoda Insecta Hemiptera Auchenorrhyncha Delphacidae Delphacidae 1 0 4 1 0 0 151235 12-35 Arthropoda Malacostraca Tanaidacea Tanaidacea 1 0 0 0 0 0 151235 12-35 Mollusca Gastropoda Ellobiidae Ellobiidae 0 1 0 0 0 0 151235 12-35 Arthropoda Insecta Diptera Diptera 0 0 1 0 0 0 151235 12-35 Arthropoda Insecta Hemiptera Heteroptera Miridae Miridae 0 0 1 0 0 0 151235 12-35 Arthropoda Insecta Hemiptera Sternorrhyncha Aphididae Aphididae 0 0 1 0 0 0 151235 12-35 Arthropoda Malacostraca Decapoda Palaemonidae Palaemonetes pugio Palaemonetes pugio 0 0 2 0 0 0 160800 8-0 Mollusca Bivalvia Mytiloida Mytilidae Geukensia demissa Geukensia demissa 0 0 0 0 1 0 160800 8-0 Arthropoda Insecta Hemiptera Heteroptera Miridae Miridae 0 0 0 0 2 0 160800 8-0 Arthropoda Arachnida Araneae Araneae 0 0 0 1 3 2 160800 8-0 Annelida Clitellata Hirudinea Hirudinea 0 0 0 1 0 0 160800 8-0 Mollusca Gastropoda Littorinimorpha Littorinidae 0 0 0 1 0 0 D2: Collembola freshwater 160800 8-0 Arthropoda Insecta Collembola Collembola 0 0 0 2 0 0 sp, both in poor shape, squished D2: Probably P. pugio but 160800 8-0 Arthropoda Malacostraca Decapoda Palaemonidae Palaemonetes Palaemonetes 0 0 0 1 0 0 rostrum broken 160800 8-0 Annelida Annelida (Capitellidae/Oligochaeta type) 3 1 0 0 0 0 160800 8-0 Mollusca Gastropoda Nassariidae Nassariidae 1 0 0 0 0 0 160800 8-0 Annelida Polychaeta Scolecida Orbiniidae Orbiniidae 0 3 0 0 0 0 160800 8-0 Annelida Polychaeta Spionida Spionidae Spionidae 0 4 0 0 0 0 160814 8-14 Arthropoda Malacostraca Amphipoda Talitridae Talitridae 0 0 0 0 5 0 160814 8-14 Mollusca Bivalvia Mytiloida Mytilidae Geukensia demissa Geukensia demissa 0 0 0 0 0 4 160814 8-14 Mollusca Gastropoda Ellobiidae Melampus bidentatus Melampus bidentatus 0 0 0 0 11 0 160814 8-14 Arthropoda Arachnida Araneae Araneae 0 0 0 0 0 1 160814 8-14 Mollusca Gastropoda Littorinimorpha Hydrobiidae Hydrobia totteni Hydrobia totteni 0 0 4 0 0 0 160814 8-14 Arthropoda Insecta Diptera Diptera 0 0 2 0 0 0 160814 8-14 Annelida Annelida (Capitellidae/Oligochaeta type) 6 1 0 1 0 0 160814 8-14 Annelida Polychaeta Sabellida Sabellidae Sabellidae 0 0 0 1 0 0 160814 8-14 Arthropoda (larvae) Insecta (larvae) Diptera (larvae) Diptera (larvae) 0 0 0 1 0 0 160814 8-14 Arthropoda Malacostraca Decapoda Palaemonidae Palaemonetes pugio Palaemonetes pugio 0 0 0 2 0 0 160814 8-14 Annelida Polychaeta Phyllodocida Nereididae Nereididae 4 0 0 0 0 0 160814 8-14 Annelida Polychaeta Phyllodocida Phyllodocidae Phyllodocidae 1 0 0 0 0 0 160814 8-14 Annelida Polychaeta Spionida Spionidae Spionidae 6 1 0 0 0 0 160814 8-14 Annelida Clitellata Hirudinea Hirudinea 0 1 0 0 0 0 160816 8-16 Arthropoda Malacostraca Amphipoda Talitridae Talitridae 0 0 0 0 1 1 160816 8-16 Mollusca Bivalvia Mytiloida Mytilidae Geukensia demissa Geukensia demissa 0 0 0 0 1 0 160816 8-16 Arthropoda Insecta Hemiptera Auchenorrhyncha Auchenorrhyncha 0 0 0 0 2 3 160816 8-16 Arthropoda Insecta Diptera Diptera 0 0 1 1 0 1 160816 8-16 Arthropoda Insecta Hemiptera Sternorrhyncha Aphididae Aphididae 0 0 0 0 0 1 160816 8-16 Mollusca Gastropoda Littorinimorpha Hydrobiidae Hydrobiidae 0 0 1 1 0 0 160816 8-16 Arthropoda Insecta Hemiptera Auchenorrhyncha Delphacidae Delphacidae 0 0 2 1 0 0 160816 8-16 Arthropoda Arachnida Araneae Araneae 0 0 1 0 0 0 160816 8-16 Arthropoda Malacostraca Decapoda Palaemonidae Palaemonetes pugio Palaemonetes pugio 0 0 0 2 0 0 160816 8-16 Annelida Polychaeta Phyllodocida Nereididae Nereididae 2 2 0 0 0 0 160816 8-16 Annelida Polychaeta Spionida Spionidae Spionidae 2 4 0 0 0 0 160816 8-16 Annelida Annelida (Capitellidae/Oligochaeta type) 0 7 0 0 0 0 161210 12-10 Arthropoda Malacostraca Amphipoda Talitridae Talitridae 0 0 1 0 3 2 161210 12-10 Mollusca Gastropoda Ellobiidae Melampus bidentatus Melampus bidentatus 0 0 0 0 2 1 161210 12-10 Arthropoda Insecta Hemiptera Heteroptera Miridae Miridae 0 0 0 0 0 1 161210 12-10 Arthropoda Insecta Hemiptera Auchenorrhyncha Auchenorrhyncha 0 0 0 0 2 1 161210 12-10 Arthropoda Arachnida Araneae Araneae 0 0 0 0 2 0 161210 12-10 Arthropoda Insecta Diptera Diptera 0 0 0 0 0 2 161210 12-10 Arthropoda Insecta Coleoptera Coleoptera 0 0 0 0 1 1 161210 12-10 Arthropoda (larvae) Insecta (larvae) Diptera (larvae) Diptera (larvae) 0 0 1 1 0 0 161210 12-10 Arthropoda Malacostraca Decapoda Palaemonidae Palaemonetes pugio Palaemonetes pugio 0 0 6 1 0 0 161210 12-10 Mollusca Gastropoda Neogastropoda Nassariidae Nassariidae 0 0 0 3 0 0 161210 12-10 Annelida Polychaeta Phyllodocida Nereididae Nereididae 1 1 0 0 0 0 161210 12-10 Annelida Annelida (Capitellidae/Oligochaeta type) 0 1 0 0 0 0 161255 12-55 Arthropoda Malacostraca Amphipoda Talitridae Talitridae 0 0 2 0 4 0 161255 12-55 Mollusca Gastropoda Ellobiidae Melampus bidentatus Melampus bidentatus 0 0 0 0 30 12 161255 12-55 Arthropoda Insecta Hemiptera Auchenorrhyncha Cicadellidae Cicadellidae 0 0 0 0 2 0 161255 12-55 Arthropoda Arachnida Araneae Araneae 0 0 1 0 1 0 161255 12-55 Arthropoda Insecta Coleoptera Coleoptera 0 0 0 0 4 0 161255 12-55 Arthropoda Insecta Diptera Diptera 0 0 3 0 0 0 161255 12-55 Arthropoda Insecta Hemiptera Auchenorrhyncha Delphacidae Delphacidae 0 0 6 0 0 0 161255 12-55 Annelida Clitellata Oligochaeta Oligochaeta 0 1 0 0 0 0 161255 12-55 Annelida Annelida (Capitellidae/Oligochaeta type) 0 3 0 0 0 0 161255 12-55 Annelida Polychaeta Scolecida Capitellidae Capitellidae 0 2 0 0 0 0 161255 12-55 Annelida Polychaeta Spionida Spionidae Streblospio benedicti 0 1 0 0 0 0 161256 12-56 Arthropoda Malacostraca Amphipoda Talitridae Talitridae 0 0 0 0 0 5 161256 12-56 Mollusca Gastropoda Ellobiidae Melampus bidentatus Melampus bidentatus 0 0 0 0 0 8 161256 12-56 Arthropoda Insecta Hemiptera Heteroptera Miridae Miridae 0 0 0 0 1 0 161256 12-56 Arthropoda Arachnida Araneae Araneae 0 0 0 0 0 2 161256 12-56 Arthropoda Insecta Diptera Diptera 0 0 0 1 0 0 161256 12-56 Arthropoda Malacostraca Decapoda Palaemonidae Palaemonetes pugio Palaemonetes pugio 0 0 0 1 0 0 161256 12-56 Annelida Polychaeta Phyllodocida Nereididae Nereididae 1 0 0 0 0 0 161256 12-56 Annelida Clitellata Oligochaeta Oligochaeta 0 1 0 0 0 0 161256 12-56 Arthropoda Malacostraca Isopoda Anthuridae Cyathura polita Cyathura polita 0 1 0 0 0 0 161270 12-70 Arthropoda Malacostraca Amphipoda Talitridae Talitridae 0 0 0 0 0 4 161270 12-70 Mollusca Bivalvia Mytiloida Mytilidae Geukensia demissa Geukensia demissa 0 0 0 0 0 3 161270 12-70 Mollusca Gastropoda Ellobiidae Melampus bidentatus Melampus bidentatus 0 0 0 0 189 150 161270 12-70 Arthropoda Insecta Hemiptera Hemiptera 0 0 0 0 1 0 161270 12-70 Arthropoda Insecta Hemiptera Auchenorrhyncha Auchenorrhyncha 0 0 0 0 0 3 161270 12-70 Arthropoda Arachnida Araneae Araneae 0 0 0 0 1 7 161270 12-70 Arthropoda Arachnida Acari Acari 0 0 0 0 0 1 Q2: "mite" 161270 12-70 Arthropoda Insecta Coleoptera Coleoptera 0 0 0 0 1 0 Q1: "weevil" 161270 12-70 Arthropoda Insecta Hymenoptera Formicidae Formicidae 0 0 0 0 0 1 161270 12-70 Arthropoda (larvae) Insecta (larvae) Diptera (larvae) Diptera (larvae) 0 0 0 0 0 1 161270 12-70 Arthropoda Insecta Diptera Diptera 0 0 2 4 0 0 161270 12-70 Arthropoda Malacostraca Decapoda Palaemonidae Palaemonetes pugio Palaemonetes pugio 0 0 1 1 0 0 161270 12-70 Arthropoda Insecta Hymenoptera Hymenoptera 0 0 0 1 0 0 161270 12-70 Arthropoda Insecta Hemiptera Auchenorrhyncha Cicadellidae Cicadellidae 0 0 0 2 0 0 161270 12-70 Annelida Polychaeta Spionida Spionidae Spionidae 0 1 0 0 0 0 161270 12-70 Annelida Polychaeta Terebellida Ampharetidae Ampharetidae 0 1 0 0 0 0 161270 12-70 Arthropoda Ostracoda Ostracoda 0 1 0 0 0 0 161270 12-70 Arthropoda Malacostraca Isopoda Anthuridae Cyathura polita Cyathura polita 0 2 0 0 0 0 A2: sample lost, was not 161274 12-74 Arthropoda Malacostraca Amphipoda Talitridae Talitridae 0 NA 0 1 28 1 sorted/ided A2: sample lost, was not 161274 12-74 Mollusca Bivalvia Mytiloida Mytilidae Geukensia demissa Geukensia demissa 0 NA 0 0 3 1 sorted/ided A2: sample lost, was not 161274 12-74 Mollusca Gastropoda Ellobiidae Melampus bidentatus Melampus bidentatus 0 NA 0 0 87 0 sorted/ided A2: sample lost, was not 161274 12-74 Arthropoda Insecta Hemiptera Sternorrhyncha Aphididae Aphididae 0 NA 0 0 0 1 sorted/ided A2: sample lost, was not 161274 12-74 Arthropoda Insecta Hemiptera Auchenorrhyncha Auchenorrhyncha 0 NA 0 0 0 3 sorted/ided A2: sample lost, was not 161274 12-74 Arthropoda Arachnida Araneae Araneae 0 NA 0 0 1 0 sorted/ided A2: sample lost, was not 161274 12-74 Arthropoda (larvae) Insecta (larvae) Coleoptera (larvae) Coleoptera (larvae) 0 NA 1 0 0 0 sorted/ided A2: sample lost, was not 161274 12-74 Arthropoda Malacostraca Decapoda Palaemonidae Palaemonetes pugio Palaemonetes pugio 0 NA 29 3 0 0 sorted/ided A2: sample lost, was not 161274 12-74 Arthropoda Insecta Hymenoptera Hymenoptera 0 NA 0 1 0 0 sorted/ided Date Time Time Marsh Tide Salintiy Date Sorted YrPlotID PlotID Name Town Date Collected Collected 2 Collected Collected 2 Collector State Salinity 2 Date Sorted 2 Sorter Date Id Identifier Comments 150802 8-2 Pine Island Newbury 9/17/2015 10:01 AM MC/NS Flood 17 10/20/2015 AP/CK 11/17/2015 AP/CK 150807 8-7 North Pool PRNWR - USFWS Rowley 9/29/2015 9:05 AM MK/MC Flood 32 10/28/2015 AP/CK 11/12/2015 AP 151202 12-2 MBL 1 Newbury 8/19/2015 1:45 PM AP/CG Flood 11 10 10/27/2015 AP/CK 11/12/2015 AP 151203 12-3 Kent's Island Rd Newbury 9/16/2015 11:05 AM AP/MC Flood 15 15 10/28/2015 AP/CK 11/12/2015 AP 151204 12-4 Red Gate Marsh 2 Newbury 8/20/2015 12:02 PM MC/NS Flood 23 24 10/20/2015 MK/AP 11/12/2015 AP 151214 12-14 Newman Rd 3 Newbury 8/17/2015 2:09 PM MC/NS Flood 20 26 10/21/2015 10/27/2015 MK/AP/CK 11/12/2015 AP 151215 12-15 Newman Rd 1 Newbury 8/14/2015 11:51 AM AP/MC/KM Flood 15 10/21/2015 10/27/2015 MK/AP/CK 11/17/2015 CK 151218 12-18 Newman Rd 2 Newbury 8/17/2015 11:23 AM MC/NS Flood 11/4/2015 AP/CK 11/17/2015 AP/CK 151221 12-21 Red Gate Marsh 1 Rowley 8/20/2015 3:31 PM MC/NS Flood 14 15 10/21/2015 MK/AP 11/18/2015 CK

Dnets taken on 10/17/15, salinity 1 taken on 10/7/15, 151222 12-22 Town Farm Rd Ipswich 9/23/2015 10/7/2015 11:52 AM MK/MC Ebb 20 19 10/27/2015 AP/CK 11/18/2015 AP/CK salinity 2 taken on 9/23/15 151232 12-32 Perley's Marina Rowley 9/23/2015 9:32 AM MC/AG Ebb 24 24 10/20/2015 MK/AP 11/18/2015 CK 151235 12-35 MBL 2 Newbury 8/19/2015 11:30 AM AP/CG Flood 17 17 11/4/2015 AP/CK 11/18/2015 AP/CK 160800 8-0 Stackyard Rd Rowley 8/18/2016 9:30 AM AP/AG Flood 31 33 9/26/2016 10/18/2016 AP/CK 11/2/2016 AP D1 empty 160814 8-14 Cottage Rd Newbury 9/2/2016 10:00 AM AP/CK Flood 34 35 10/18/2016 AP/CK 11/2/2016 AP 160816 8-16 Old Pine Island Rd Newbury 9/1/2016 9:30 AM AP/CK Flood 32 33 10/18/2016 10/25/2016 AG/CK 11/2/2016 AP 161210 12-10 Parker River 1 Newbury 9/7/2016 12:30 PM AP/CK/BB Flood 34 34 10/25/2016 AG/CK 11/2/2016 AP Fiddler crab in burrow 161255 12-55 Kent's Island Newbury 8/25/2016 8:10 AM AP/CK/AG Ebb 32 33 10/18/2016 AP/CK 11/2/2016 AP/CK D2 empty, A1 empty

station 1 salinity read 23/24 161256 12-56 Little River Newbury 8/8/2016 not recorded AP/CK Flood 25 25 9/26/2016 AP/CK 11/2/2016 CK ppt first time; D1 empty 161270 12-70 Parker River Newbury 8/17/2016 9:00 AM AP/CK/AG Flood 28 29 9/26/2016 AP/CK 11/2/2016 CK A1 empty

A2 sample was accidentally put in trash before sorting, 161274 12-74 Parker River 2 Newbury 9/7/2016 2:15 PM AP/CK/BB Flood 31 33 10/25/2016 AG/CK 11/2/2016 CK sample lost; A1 empty Collector Code Code Collector AP Adrienne Pappal MC Marc Carullo MK Melanie Kenney AG Ashley Green CK Cristina Kennedy CG Chris Garby NS Nick S KM? BB Bob Boeri

Metadata Data: Name Data: Type Data: Definition YrPlotID Text Year of sampling (xx) with stratified random sampling bin (xx) and index (xx) PlotID Text ID for each sample plot (bin,"-",index) Phylum Text Class Text Order Text Suborder Text Family Text Genus Text Species Text FinalID Text Taxanomic group of the lowest rank ID A-1 Number Number of organisms in Auger Sample 1 A-2 Number Number of organisms in Auger Sample 2 D-1 Number Number of organisms in Dip Net Sample 1 D-2 Number Number of ogranisms in Dip Net Sample 2 Q-1 Number Number of organisms in Quadrat Sample (Aggregate) Q-2 Number Number of organisms in Quadrat Sample (Aggregate) FinalID Phylum Class Subclass Order SubOrder Family Genus Species Acari Arthropoda Arachnida Acari Actinaria Cnidaria Anthozoa Actiniaria Ampeliscidae Arthropoda Malacostraca Amphipoda Ampeliscidae Ampharetidae Annelida Polychaeta Terebellida Ampharetidae Amphipoda Arthropoda Malacostraca Amphipoda Annelida (Capitellidae/OligochaetaAnnelida type) Annelida (Polychaeta) Annelida Annelida (unknown) Annelida Anthuridae Arthropoda Malacostraca Isopoda Anthuridae Anurida maritima Arthropoda Insecta Collembola Neanuridae Anurida maritima Aoridae Arthropoda Malacostraca Amphipoda Aoridae Aphididae Arthropoda Insecta Hemiptera Sternorrhyncha Aphididae Araneae Arthropoda Arachnida Araneae Arenicolidae Annelida Polychaeta Scolecida Arenicolidae Auchenorrhyncha Arthropoda Insecta Hemiptera Auchenorrhyncha Belostomatidae Arthropoda Insecta Pterygota Hemiptera Heteroptera Belostomatidae Bivalvia Mollusca Bivalvia Branchiura Arthropoda Maxillopoda Capitellidae Annelida Polychaeta Scolecida Capitellidae Carabidae Arthropoda Insecta Coleoptera Carabidae Carcinus maenas Arthropoda Malacostraca Decapoda Portunidae Carcinus maenas Ceratopogonidae Arthropoda Insecta Diptera Ceratopogonidae Ceratopogonidae (larvae) Arthropoda (larvae) Insecta (larvae) Diptera (larvae) Ceratopogonidae (larvae) Chironomidae Arthropoda Insecta Diptera Chironomidae Chironomidae (larvae) Arthropoda (larvae) Insecta (larvae) Diptera (larvae) Chironomidae (larvae) Cicadellidae Arthropoda Insecta Hemiptera Auchenorrhyncha Cicadellidae Clubionidae Arthropoda Arachnida Araneae Clubionidae Coccinellidae Arthropoda Insecta Coleoptera Coccinellidae Coleoptera Arthropoda Insecta Coleoptera Coleoptera (larvae) Arthropoda (larvae) Insecta (larvae) Coleoptera (larvae) Coleoptera (pupae) Arthropoda (pupae) Insecta (pupae) Coleoptera (pupae) Collembola Arthropoda Insecta Collembola Corixidae Arthropoda Insecta Hemiptera Heteroptera Corixidae Corophiidae Arthropoda Malacostraca Amphipoda Corophiidae Crangonidae Arthropoda Malacostraca Decapoda Crangonidae Culicidae Arthropoda Insecta Diptera Culicidae Cumacea Arthropoda Malacostraca Cumacea Curculionidae Arthropoda Insecta Coleoptera Curculionidae Cyathura polita Arthropoda Malacostraca Isopoda Anthuridae Cyathura polita Cylichnidae Mollusca Gastropoda Cephalaspidea Cylichnidae Decapoda Arthropoda Malacostraca Decapoda Delphacidae Arthropoda Insecta Hemiptera Auchenorrhyncha Delphacidae Dermaptera Arthropoda Insecta Dermaptera Diadumene lineata Cnidaria Anthozoa Actiniaria Diadumenidae Diadumene lineata Diptera Arthropoda Insecta Diptera Diptera (larvae) Arthropoda (larvae) Insecta (larvae) Diptera (larvae) Diptera (pupae) Arthropoda (pupae) Insecta (pupae) Diptera (pupae) Dolichopodidae Arthropoda Insecta Diptera Dolichopodidae Dorvilleidae Annelida Polychaeta Eunicida Dorvilleidae Edwardsiidae Cnidaria Anthozoa Actiniaria Edwardsiidae Ellobiidae Mollusca Gastropoda Ellobiidae Ephydridae Arthropoda Insecta Diptera Ephydridae Ephydridae (larave) Arthropoda (larvae) Insecta (larvae) Diptera (larvae) Ephydridae (larvae) Eunicidae Annelida Polychaeta Eunicida Eunicidae Formicidae Arthropoda Insecta Hymenoptera Formicidae Gammaridae Arthropoda Malacostraca Amphipoda Gammaridae Gastropoda Mollusca Gastropoda Georyssidae Arthropoda Insecta Coleoptera Georyssidae Gerridae Arthropoda Insecta Hemiptera Heteroptera Gerridae Geukensia demissa Mollusca Bivalvia Mytiloida Mytilidae Geukensia demissa Gomphidae (larvae) Arthropoda (larvae) Insecta (larvae) Odonata (larvae) Gomphidae (larvae) Gryllidae Arthropoda Insecta Orthoptera Gryllidae Hemigrapsus sanguineus Arthropoda Malacostraca Decapoda Varunidae Hemigrapsus sanguineus Hemiptera Arthropoda Insecta Hemiptera Heteroptera Arthropoda Insecta Hemiptera Heteroptera Hirudinea Annelida Clitellata Hirudinea Hydrobia totteni Mollusca Gastropoda Littorinimorpha Hydrobiidae Hydrobia totteni Hydrobiidae Mollusca Gastropoda Littorinimorpha Hydrobiidae Hydrophilidae (larvae) Arthropoda (larvae) Insecta (larvae) Coleoptera (larvae) Hydrophilidae (larvae) Hydropsychidae Arthropoda Insecta Trichoptera Hydropsychidae Hydropsychidae (larvae) Arthropoda (larvae) Insecta (larvae) Trichoptera (larvae) Hydropsychidae (larvae) Hydropsychidae (pupae) Arthropoda (pupae) Insecta (pupae) Trichoptera (pupae) Hydropsychidae (pupae) Hydroptilidae Arthropoda Insecta Trichoptera Hydroptilidae Hymenoptera Arthropoda Insecta Hymenoptera Hymenoptera (larvae) Arthropoda (larvae) Insecta (larvae) Hymenoptera (larvae) Hymenoptera (pupae) Arthropoda (pupae) Insecta (pupae) Hymenoptera (pupae) Idoteidae Arthropoda Malacostraca Isopoda Idoteidae Ilyanassa Mollusca Gastropoda Neogastropoda Nassariidae Ilyanassa Insecta Arthropoda Insecta Insecta (larvae) Arthropoda (larvae) Insecta (larvae) Insecta (pupae) Arthropoda (pupae) Insecta (pupae) Isopoda Arthropoda Malacostraca Isopoda Janiridae Arthropoda Malacostraca Isopoda Janiridae Lepidoptera Arthropoda Insecta Lepidoptera Lepidoptera (larvae) Arthropoda (larvae) Insecta (larvae) Lepidoptera (larvae) Limulus polyphemus Arthropoda Merostomata Xiphosurida Limulidae Limulus polyphemus Linyphiidae Arthropoda Arachnida Araneae Linyphiidae Littorina saxatilis Mollusca Gastropoda Littorinimorpha Littorinidae Littorinidae Mollusca Gastropoda Littorinimorpha Littorinidae Lumbrineridae Annelida Polychaeta Eunicida Lumbrineridae Lycosidae Arthropoda Arachnida Araneae Lycosidae Mactridae Mollusca Bivalvia Veneroida Mactridae Maldanidae Annelida Polychaeta Scolecida Maldanidae Mantodea Arthropoda Insecta Dictyoptera Mantodea Melampus bidentatus Mollusca Gastropoda Ellobiidae Melampus bidentatus Melitidae Arthropoda Malacostraca EumalacostracaAmphipoda Gammaridea Melitidae Micryphantidae Arthropoda Arachnida Araneae Micryphantidae Miridae Arthropoda Insecta Hemiptera Heteroptera Miridae Molgula Chordata Ascidiacea Stolidobranchia Molgulidae Molgula Mycetophilidae Arthropoda Insecta Diptera Mycetophilidae Myidae Mollusca Bivalvia Myoida Myidae Mytilidae Mollusca Bivalvia Mytiloida Mytilidae Nassariidae Mollusca Gastropoda Neogastropoda Nassariidae Nematoda Nematoda Nemertea Nemertea Nephtyidae Annelida Polychaeta Phyllodocida Nephtyidae Nereididae Annelida Polychaeta Phyllodocida Nereididae Ocypodidae Arthropoda Malacostraca Decapoda Ocypodidae Oenonidae Annelida Polychaeta Eunicida Oenonidae Oligochaeta Annelida Clitellata Oligochaeta Oniscidae Arthropoda Malacostraca Isopoda Oniscidae Opheliidae Annelida Polychaeta Scolecida Opheliidae Orbiniidae Annelida Polychaeta Scolecida Orbiniidae Orthoptera Arthropoda Insecta Orthoptera Ostracoda Arthropoda Ostracoda Palaemonetes Arthropoda Malacostraca Decapoda Palaemonidae Palaemonetes Palaemonetes pugio Arthropoda Malacostraca Decapoda Palaemonidae Palaemonetes pugio Palaemonidae Arthropoda Malacostraca Decapoda Palaemonidae Palemon elegans Arthropoda Malacostraca Decapoda Palaemonidae Palaemon elegans Panopeidae Arthropoda Malacostraca Decapoda Panopeidae Paraonidae Annelida Polychaeta Scolecida Paraonidae Pectinariidae Annelida Polychaeta Terebellida Phyllodocidae Annelida Polychaeta Phyllodocida Phyllodocidae Piscicolidae Annelida Clitellata Hirudinea Rhynchobdellida Piscicolidae Platygastridae Arthropoda Insecta Hymenoptera Platygastridae Poduridae Arthropoda Insecta Collembola Poduridae Polychaeta Annelida Polychaeta Portunidae Arthropoda Malacostraca Decapoda Portunidae Pseudoscorpiones Arthropoda Arachnida Pseudoscorpiones Pycnogonida Arthropoda Pycnogonida Rhagionidae Arthropoda Insecta Diptera Rhagionidae Rhithropanopeus harrisii Arthropoda Malacostraca EumalacostracaDecapoda Pleocyemata Panopeidae Rhithropanopeus harrisii Rhyacophilidae Arthropoda Insecta Trichoptera Rhyacophilidae Sabellidae Annelida Polychaeta Sabellida Sabellidae Saldidae Arthropoda Insecta Hemiptera Heteroptera Saldidae Scalibregmatidae Annelida Polychaeta Scolecida Scalibregmatidae Sessilia Arthropoda Maxillopoda Sessilia Spionidae Annelida Polychaeta Spionida Spionidae Sternorrhyncha Arthropoda Insecta Hemiptera Sternorrhyncha Stratiomyidae Arthropoda (larvae) Insecta (larvae) Pterygota (larvae)Diptera (larvae) Brachycera (larvae) Stratiomyidae Streblospio benedicti Annelida Polychaeta Spionida Spionidae Streblospio benedicti Syllidae Annelida Polychaeta Phyllodocida Syllidae Tabanidae Arthropoda Insecta Diptera Tabanidae Tabanidae (larvae) Arthropoda (larvae) Insecta (larvae) Diptera (larvae) Tabanidae (larvae) Talitridae Arthropoda Malacostraca Amphipoda Talitridae Tanaidacea Arthropoda Malacostraca Tanaidacea Tellinidae Mollusca Bivalvia Veneroida Tellinidae Terebellidae Annelida Polychaeta Terebellida Terebellidae Tetragnathidae Arthropoda Arachnida Araneae Tetragnathidae Tettigoniidae Arthropoda Insecta Orthoptera Tettigoniidae Tipulidae Arthropoda Insecta Diptera Tipulidae Tipulidae (larvae) Arthropoda (larvae) Insecta (larvae) Diptera (larvae) Tipulidae (larvae) Tipulidae (pupae) Arthropoda (pupae) Insecta (pupae) Diptera (pupae) Tipulidae (pupae) Trichoptera Arthropoda Insecta Trichoptera Trichoptera (larvae) Arthropoda (larvae) Insecta (larvae) Trichoptera (larvae) Mollusca Bivalvia Veneroida Veneridae VEGETATION Summary of Method: A single bayonet (vertical projection) is used to record species at fixed intervals along each transect. Walk along the transect opposite the side used to lie the transect to avoid trampling samples. Set the transect interval by dividing the transect length by 10; this will produce 11 point intercept locations along each transect. As stated in section 8.1, the minimum and maximum vegetation transect lengths will be 30 m and 50 m, respectively. Tally each plant species intercepted by the bayonet from marsh surface to canopy at each fixed interval along the transect. Record the transect length and interval along with species occurrence on the Vegetation Form (QAPP, Appendix C). Note, GPS point locations taken at the 0 m along each transect are in geographic coordinates (decimal degrees), WGS 84. ID YrPlotID PlotID Name Town DateSamp Evaluators SiteComm Lat1 Lon1 Time1 Dis1 Len1 Com1 Int1 Comment1 Lat2 Lon2 Time2 Dis2 Len2 Com2 Int2 Comment2 Lat3 Lon3 Time3 Dis3 Len3 Com3 Int3 Comment3 205 150802 8-2 Pine Island Newbury 09/17/15 MK, MC 42.78305000 -70.82995000 9:45 50 50 114 5.0 42.78323333 -70.83058333 42.78305000 -70.83121667 207 150807 8-7 North Pool PRNWR - USFWS Rowley 09/17/15 MM, MK, NS 42.74548333 -70.80676667 13:55 35 50 182 5.0 42.74543333 -70.80720000 13:30 0 50 182 5.0 42.74525000 -70.80758333 12:55 50 35 182 5.0 208 151202 12-2 MBL 1 Newbury 08/19/15 MK, MC 42.75711667 -70.88576667 50 50 347 5.0 42.75736667 -70.88525000 14:17 0 50 374 5.0 42.75756667 -70.88468333 13:44 50 50 347 5.0 209 151203 12-3 Kent's Island Rd Newbury 09/16/15 MK, MC 42.76741667 -70.87923333 50 50 121 5.0 42.76700000 -70.87946667 11:35 0 50 121 5.0 42.76651667 -70.87946667 11:45 50 50 121 5.0 210 151204 12-4 Red Gate Marsh 2 Newbury 08/20/15 CG, MM 42.74915000 -70.86618333 12:00 45 50 260 5.0 42.74953333 -70.86648333 12:30 0 50 260 5.0 42.74991667 -70.86663333 12:45 45 50 260 5.0 211 151214 12-14 Newman Rd 3 Newbury 08/17/15 MK, MM 42.76680000 -70.85798333 15:00 50 50 132 5.0 42.76638333 -70.85821667 0 50 132 5.0 42.76598333 -70.85850000 14:00 50 50 131 5.0 212 151215 12-15 Newman Rd 1 Newbury 08/14/15 MK, MC 42.77540000 -70.86010000 12:12 35 50 157 5.0 42.77516667 -70.86041667 13:04 0 50 157 5.0 42.77498333 -70.86073333 14:39 35 50 157 5.0 213 151218 12-18 Newman Rd 2 Newbury 08/17/15 MK, MM 42.77116837 -70.85858479 40 50 124 5.0 42.77085000 -70.85876667 11:20 0 50 124 5.0 42.77048963 -70.85887531 10:59 40 50 124 5.0 214 151221 12-21 Red Gate Marsh 1 Rowley 08/20/15 CG, MM 42.74298333 -70.86968333 15:02 50 50 3 5.0 42.74331667 -70.86926667 15:18 0 50 3 5.0 42.74360000 -70.86881667 15:50 50 50 3 5.0 215 151222 12-22 Town Farm Rd Ipswich 09/23/15 42.70586667 -70.84990000 12:38 50 50 32 5.0 42.70575000 -70.84931667 11:58 0 50 32 5.0 42.70566667 -70.84871667 11:35 50 50 32 5.0 216 151232 12-32 Perley's Marina Rowley 09/23/15 MK, MM 42.71955000 -70.86158333 9:00 50 50 32 5.0 42.71945000 -70.86098333 9:20 0 50 32 5.0 42.71931667 -70.86041667 9:40 50 50 32 5.0 217 151235 12-35 MBL 2 Newbury 08/19/15 MK, MC 42.76266667 -70.88551667 12:30 35 50 159 5.0 42.76250000 -70.88588333 0 50 159 5.0 42.76226667 -70.88618333 11:27 35 50 159 5.0 218 160800 8-0 Stackyard Rd Rowley 08/18/16 MC, CG, CK 42.73500466 -70.84622003 9:24 44 50 83 5.0 42.73466667 -70.84601667 10:01 0 50 83 5.0 42.73425000 -70.84565000 10:30 50 50 83 5.0 219 160814 8-14 Cottage Rd Newbury 09/02/16 MC, AG 42.76523333 -70.82521667 50 50 52 5.0 42.76493333 -70.82481667 10:49 0 50 52 5.0 42.76473333 -70.82425000 50 50 52 5.0 T1 point T3 point taken 4 m taken 3.5 m 220 160816 8-16 Old Pine Island Rd Newbury 09/01/16 MC, AG 42.77550000 -70.82591667 40 50 25 5.0 from bank, 42.77555000 -70.82540000 9:58 0 50 25 5.0 42.77543333 -70.82495000 40 50 25 5.0 from bank, which was which was inaccessible inaccessble 221 161210 12-10 Parker River 1 Newbury 09/07/16 MC, AG 42.76001667 -70.85785000 12:23 50 50 270 5.0 42.76043333 -70.85793333 0 50 270 5.0 42.76108333 -70.85701667 50 50 270 5.0

Large areas dominated by S. depressa, some completely senesced and desicated (branching form--formerly S. 222 161255 12-55 Kent's Island Newbury 08/25/16 CG, MC 42.76551667 -70.86513333 8:44 50 50 339 5.0 42.76558333 -70.86448333 8:18 0 50 339 5.0 42.76596667 -70.86413333 7:58 50 50 339 5.0 europaea), with large mats of singular, erect S. depressa (formerly S. virginica)--the latter being reddish/green.

223 161256 12-56 Little River Newbury 08/08/16 MC, AG 42.77506667 -70.86811667 14:06 50 50 304 5.0 42.77556667 -70.86811667 14:45 0 50 304 5.0 42.77595000 -70.86773333 15:23 50 50 304 5.0 224 161270 12-70 Parker River Newbury 08/17/16 CG, MC 42.75493333 -70.87948333 8:40 50 50 183 5.0 42.75481667 -70.88006667 9:15 0 50 183 5.0 42.75480000 -70.88075000 9:39 50 50 183 5.0

T1 and T2 were 75% very wet and mucky. Possible 225 161274 12-74 Parker River 2 Newbury 09/07/16 MC, AG, RB 42.76111667 -70.86628333 13:56 50 50 97 5.0 42.76066667 -70.86631667 0 50 97 5.0 42.76021667 -70.86618333 50 50 97 5.0 oversaturation due to plugged, remnant ditches ID YrPlotID PlotID Genus Species T_Sum T1 T2 T3 1295 150802 8-2 Distichlis spicata 19 8 4 7 1296 150802 8-2 Glaux maritima 9 1 4 4 1297 150802 8-2 Iva frutescens 2 1 0 1 1298 150802 8-2 Solidago sempirvirens 3 0 0 3 1299 150802 8-2 Spartina alterniflora-short 6 0 5 1 1300 150802 8-2 Spartina alterniflora-tall 5 2 1 2 1301 150802 8-2 Spartina patens 20 4 8 8 1302 150807 8-7 Limonium carolinianum 1 0 0 1 1303 150807 8-7 Spartina alterniflora-short 22 7 7 8 1304 150807 8-7 Spartina alterniflora-tall 7 1 4 2 1305 150807 8-7 Spartina patens 20 10 7 3 1306 150807 8-7 Suaeda 2 0 0 2 1307 151202 12-2 Amaranthus cannabinus 1 0 0 1 1308 151202 12-2 Argentina anserina 2 0 1 1 1309 151202 12-2 Distichlis spicata 11 6 4 1 1310 151202 12-2 Juncus gerardii 6 0 2 4 1311 151202 12-2 Spartina alterniflora-short 8 2 3 3 1312 151202 12-2 Spartina alterniflora-tall 1 0 0 1 1313 151202 12-2 Spartina patens 24 6 11 7 1314 151202 12-2 Spartina pectinata 2 0 1 1 1315 151202 12-2 Other panne 3 3 0 0 1316 151203 12-3 Amaranthus cannabinus 2 1 1 0 1317 151203 12-3 Atriplex 3 0 0 3 1318 151203 12-3 Distichlis spicata 24 7 10 7 1319 151203 12-3 Juncus gerardii 10 2 4 4 1320 151203 12-3 Spartina alterniflora-short 4 2 0 2 1321 151203 12-3 Spartina alterniflora-tall 2 1 1 0 1322 151203 12-3 Spartina patens 11 7 3 1 1323 151204 12-4 Atriplex 1 1 0 0 1324 151204 12-4 Distichlis spicata 11 2 3 6 1325 151204 12-4 Iva frutescens 1 0 0 1 1326 151204 12-4 Spartina alterniflora-short 23 10 7 6 1327 151204 12-4 Spartina alterniflora-tall 4 1 2 1 1328 151204 12-4 Spartina patens 20 7 8 5 1329 151204 12-4 Other marsh dieback 1 0 1 0 1330 151214 12-14 Distichlis spicata 6 4 0 2 1331 151214 12-14 Spartina alterniflora-short 27 10 10 7 1332 151214 12-14 Spartina alterniflora-tall 2 0 0 2 1333 151214 12-14 Spartina patens 20 4 8 8 1334 151215 12-15 Argentina anserina 3 2 0 1 1335 151215 12-15 Atriplex 1 1 0 0 1336 151215 12-15 Distichlis spicata 6 2 1 3 1337 151215 12-15 Juncus gerardii 7 4 0 3 1338 151215 12-15 Quercus alba 1 0 0 1 1339 151215 12-15 Schoenoplectus pungens 1 1 0 0 1340 151215 12-15 Schoenoplectus robustus 2 0 0 2 1341 151215 12-15 Solidago sempirvirens 3 2 0 1 1342 151215 12-15 Spartina alterniflora-short 12 5 4 3 1343 151215 12-15 Spartina alterniflora-tall 8 2 4 2 1344 151215 12-15 Spartina patens 18 7 7 4 1345 151215 12-15 Spartina pectinata 1 0 1 0 1346 151215 12-15 Typha latifolia 5 0 3 2 1347 151215 12-15 Other marsh dieback 2 1 0 1 1348 151218 12-18 Amaranthus cannabinus 2 1 1 0 1349 151218 12-18 Atriplex 2 0 2 0 1350 151218 12-18 Distichlis spicata 9 5 2 2 1351 151218 12-18 Salicornia depressa 1 0 1 0 1352 151218 12-18 Spartina alterniflora-short 22 5 9 8 1353 151218 12-18 Spartina alterniflora-tall 3 2 1 0 1354 151218 12-18 Spartina patens 17 6 5 6 1355 151221 12-21 Distichlis spicata 4 4 0 0 1356 151221 12-21 Juncus gerardii 2 0 2 0 1357 151221 12-21 Phragmites australis 6 0 0 6 1358 151221 12-21 Schoenoplectus robustus 1 0 0 1 1359 151221 12-21 Spartina alterniflora-short 14 6 7 1 1360 151221 12-21 Spartina alterniflora-tall 1 1 0 0 1361 151221 12-21 Spartina patens 24 5 10 9 1362 151221 12-21 Typha angustifolia 1 0 1 0 1363 151222 12-22 Distichlis spicata 8 3 2 3 1364 151222 12-22 Iva frutescens 1 0 0 1 1365 151222 12-22 Spartina alterniflora-short 17 8 6 3 1366 151222 12-22 Spartina alterniflora-tall 1 0 0 1 1367 151222 12-22 Spartina patens 16 6 5 5 1368 151222 12-22 Other panne 7 0 2 5 1369 151232 12-32 Atriplex 1 0 1 0 1370 151232 12-32 Distichlis spicata 7 2 3 2 1371 151232 12-32 Spartina alterniflora-short 19 8 6 5 1372 151232 12-32 Spartina alterniflora-tall 7 1 2 4 1373 151232 12-32 Spartina patens 28 10 9 9 1374 151235 12-35 Amaranthus cannabinus 4 1 1 2 1375 151235 12-35 Argentina anserina 1 0 0 1 1376 151235 12-35 Atriplex 0 0 0 0 1377 151235 12-35 Distichlis spicata 18 7 9 2 1378 151235 12-35 Juncus gerardii 23 6 8 9 1379 151235 12-35 Spartina alterniflora-short 14 2 3 9 1380 151235 12-35 Spartina alterniflora-tall 2 1 1 0 1381 151235 12-35 Spartina patens 13 3 2 8 1382 151235 12-35 Triglochin maritimum 1 0 1 0 1383 160800 8-0 Distichlis spicata 11 5 2 4 1384 160800 8-0 Spartina alterniflora-short 11 3 4 4 1385 160800 8-0 Spartina alterniflora-tall 3 1 1 1 1386 160800 8-0 Spartina patens 28 10 9 9 1387 160814 8-14 Distichlis spicata 20 10 3 7 1388 160814 8-14 Juncus gerardii 2 2 0 0 1389 160814 8-14 Plantago maritima 1 1 0 0 1390 160814 8-14 Ruppia maritima 2 0 2 0 1391 160814 8-14 Salicornia depressa 3 0 2 1 1392 160814 8-14 Spartina alterniflora-short 7 1 2 4 1393 160814 8-14 Spartina alterniflora-tall 2 0 1 1 1394 160814 8-14 Spartina patens 20 8 6 6 1395 160814 8-14 Other creek 1 0 0 1 1396 160814 8-14 Other pool 2 0 2 0 1397 160816 8-16 Distichlis spicata 15 5 3 7 1398 160816 8-16 Glaux maritima 3 3 0 0 1399 160816 8-16 Juncus gerardii 1 1 0 0 1400 160816 8-16 Spartina alterniflora-short 11 3 6 2 1401 160816 8-16 Spartina alterniflora-tall 4 2 1 1 1402 160816 8-16 Spartina patens 22 10 4 8 1403 161210 12-10 Atriplex 1 0 0 1 1404 161210 12-10 Distichlis spicata 11 2 3 6 1405 161210 12-10 Juncus gerardii 3 0 0 3 1406 161210 12-10 Spartina alterniflora-short 12 7 5 0 1407 161210 12-10 Spartina alterniflora-tall 4 1 1 2 1408 161210 12-10 Spartina patens 13 3 5 5 1409 161210 12-10 Other algal mat 2 0 2 0 1410 161210 12-10 Other bare soil 1 1 0 0 1411 161210 12-10 Other panne 2 0 2 0 1412 161255 12-55 Distichlis spicata 5 2 2 1 1413 161255 12-55 Salicornia depressa 6 6 0 0 1414 161255 12-55 Spartina alterniflora-short 17 4 5 8 1415 161255 12-55 Spartina alterniflora-tall 3 1 1 1 1416 161255 12-55 Spartina patens 20 7 6 7 1417 161255 12-55 Other algal mat 4 0 4 0 1418 161255 12-55 Other panne 4 0 4 0 1419 161256 12-56 Argentina anserina 3 0 2 1 1420 161256 12-56 Atriplex 1 0 1 0 1421 161256 12-56 Distichlis spicata 21 8 8 5 1422 161256 12-56 Juncus gerardii 14 5 5 4 1423 161256 12-56 Spartina alterniflora-short 13 0 5 8 1424 161256 12-56 Spartina alterniflora-tall 2 2 0 0 1425 161256 12-56 Spartina patens 19 4 8 7 1426 161256 12-56 Triglochin maritimum 4 3 1 0 1427 161270 12-70 Distichlis spicata 6 6 0 0 1428 161270 12-70 Juncus gerardii 2 2 0 0 1429 161270 12-70 Salicornia depressa 5 3 1 1 1430 161270 12-70 Spartina alterniflora-short 19 4 8 7 1431 161270 12-70 Spartina alterniflora-tall 3 1 1 1 1432 161270 12-70 Spartina patens 22 5 9 8 1433 161274 12-74 Distichlis spicata 5 0 0 5 1434 161274 12-74 Juncus gerardii 1 0 0 1 1435 161274 12-74 Salicornia depressa 2 1 0 1 1436 161274 12-74 Spartina alterniflora-short 17 9 7 1 1437 161274 12-74 Spartina alterniflora-tall 3 1 1 1 1438 161274 12-74 Spartina patens 17 1 6 10 Nomenclature Changes Current Former Genus_C Species_C Genus_F Species_F Argentina anserina Potentilla anserina Atriplex Atriplex patula Juncus arcticus Juncus balticus Limonium carolinianum Limonium nashii Pluchea odorata Pluchea purpurascens Salicornia depressa Salicornia europaea Salicornia depressa Salicornia virginica Suaeda Sueda linearis Symphyotrichumsubulatum Aster subulatus Symphyotrichumtenuifolium Aster tenuifolius Thinopyrum pycnanthum Agropyron pungens

Collector Code AP Adrienne Pappal AG Ashley Green CG Chris Garby CK Cristina Kennedy MC Marc Carullo MK Melanie Kenney MM Mike McHugh NS Nick S. RB Robert Boeri Worksheet_Name Column_Name Column_Type Column_Definition Vegetation ID Text Unique identifier Vegetation YrPlotID Text Year of sampling (xx) with stratified random sampling bin (xx) and index (xx) Vegetation PlotID Text Unique ID for each sample plot Vegetation Genus Text Except where non-vascular plant or other target feature; recorded as "Other" Except where non-vascular plant or other target feature; recorded as (e.g., aquatic Vegetation Species Text algal mat, bare soil, creek, dead plant material, ditch, panne (unvegetated), pool, rock, wrack); Spartina alterniflora is always classified as short form or tall form Vegetation T_Sum Number Number of times a species occurred along all three transects within sample plot Vegetation T1 Number Number of times a species occurred along Transect 1 Vegetation T2 Number Number of times a species occurred along Transect 2 Vegetation T3 Number Number of times a species occurred along Transect 3 Plot_Record ID Text Unique identifier Plot_Record YrPlotID Text Year of sampling (xx) with stratified random sampling bin (xx) and index (xx) Plot_Record PlotID Text Unique ID for each sample plot Plot_Record Name Text CZM-defined site name Plot_Record Town Text Town in which the plot sits Plot_Record DateSamp Date First date of sampling Plot_Record Evaluators Text Marsh collectors performing an recording assessment--see Collector Code worksheet Plot_Record SiteComm Text Comments about the overall site Plot_Record Lat1 Number Transect 1 latitude in decimal degrees, using WGS84 horizontal datum Plot_Record Lon1 Number Transect 1 longitude in decimal degrees, using WGS84 horizontal datum Plot_Record Time1 Time Starting time of sampling along along Transect 1 (24-hr clock) Plot_Record Dis1 Number Distance of Transect 1 from center of baseline (i.e., Transect 2) in meters Plot_Record Len1 Number Length of Transect 1 in meters Plot_Record Com1 Number Compass bearing of Transect 1 projecting out from creek bank Plot_Record Int1 Number Length of interval used between sampling points along Transect 1 Plot_Record Comment1 Text Comments about Transect 1 Plot_Record Lat2 Number Transect 2 latitude in decimal degrees, using WGS84 horizontal datum Plot_Record Lon2 Number Transect 2 longitude in decimal degrees, using WGS84 horizontal datum Plot_Record Time2 Time Starting time of sampling along along Transect 2 (24-hr clock) Plot_Record Dis2 Number Distance of Transect 2 from center of baseline (i.e., Transect 2) in meters Plot_Record Len2 Number Length of Transect 2 in meters Plot_Record Com2 Number Compass bearing of Transect 2 projecting out from creek bank Plot_Record Int2 Number Length of interval used between sampling points along Transect 2 Plot_Record Comment2 Text Comments about Transect 2 Plot_Record Lat3 Number Transect 3 latitude in decimal degrees, using WGS84 horizontal datum Plot_Record Lon3 Number Transect 3 longitude in decimal degrees, using WGS84 horizontal datum Plot_Record Time3 Time Starting time of sampling along along Transect 3 (24-hr clock) Plot_Record Dis3 Number Distance of Transect 3 from center of baseline (i.e., Transect 2) in meters Plot_Record Len3 Number Length of Transect 3 in meters Plot_Record Com3 Number Compass bearing of Transect 3 projecting out from creek bank Plot_Record Int3 Number Length of interval used between sampling points along Transect 3 Plot_Record Comment3 Text Comments about Transect 3