International Journal of Systematic and Evolutionary Microbiology (2016), 66, 5529–5536 DOI 10.1099/ijsem.0.001552 Raineyella antarctica gen. nov., sp. nov., a psychrotolerant, D-amino-acid-utilizing anaerobe isolated from two geographic locations of the Southern Hemisphere Elena Vladimirovna Pikuta,1 Rodolfo Javier Menes,2 Alisa Michelle Bruce,3† Zhe Lyu,4 Nisha B. Patel,5 Yuchen Liu,6 Richard Brice Hoover,1 Hans-Jürgen Busse,7 Paul Alexander Lawson5 and William Barney Whitman4 Correspondence 1Department of Mathematical, Computer and Natural Sciences, Athens State University, Athens, Elena Vladimirovna Pikuta AL 35611, USA
[email protected] 2Catedra de Microbiología, Facultad de Química y Facultad de Ciencias, UDELAR, 11800 or Montevideo, Uruguay
[email protected] 3Biology Department, University of Alabama in Huntsville, Huntsville, AL 35899, USA 4Microbiology Department, University of Georgia in Athens, Athens, GA 30602, USA 5Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK 73019, USA 6Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA 7Institut für Mikrobiologie - Veterinarmedizinische€ Universitat€ Wien, A-1210 Wien, Austria A Gram-stain-positive bacterium, strain LZ-22T, was isolated from a rhizosphere of moss Leptobryum sp. collected at the shore of Lake Zub in Antarctica. Cells were motile, straight or pleomorphic rods with sizes of 0.6–1.0Â3.5–10 µm. The novel isolate was a facultatively anaerobic, catalase-positive, psychrotolerant mesophile. Growth was observed at 3–41 C (optimum 24–28 C), with 0–7 % (w/v) NaCl (optimum 0.25 %) and at pH 4.0–9.0 (optimum pH 7.8). The quinone system of strain LZ-22T possessed predominately menaquinone MK-9(H4).