International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020)
International Seminar on “Recent Advances in Bioremedial Technology’’ (RABT-2020)
31st January 2020
Proceedings of the International Seminar
Organized by
Department of Biotechnology Thiruvalluvar University (State University) Serkkadu, Vellore – 632 115, Tamil Nadu, India.
International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020)
International Seminar on “Recent Advances in Bioremedial Technology’’ (RABT-2020) Department of Biotechnology Thiruvalluvar University
Organizing Committee
Chief Patron Dr.S. THAMARAI SELVI Honourable Vice Chancellor, Thiruvalluvar University.
Paron Dr.S. Syed Shafi Registrar i/c. Thiruvalluvar University.
Program Co-ordinator Dr. Ernest David Professor & Head, Department of Biotechnology, Thiruvalluvar University.
Convener Dr. R.Babu Janarthanam Associate Professor, Department of Biotechnology, Thiruvalluvar University.
Organizing Secretaries Dr. A.Rajasekar Assistant Professor, Department of Biotechnology, Thiruvalluvar University.
Dr. M.C.Harish Assistant Professor, Department of Biotechnology, Thiruvalluvar University.
Dr. S.Vijayanand Assistant Professor, Department of Biotechnology, Thiruvalluvar University.
International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020)
International Seminar On “Recent Advances in Bioremedial Technology’’ (RABT 2020), 31st, January 2020 Organized by Department of Biotechnology, Thiruvalluvar University, Vellore – 632 115 Program Schedule
31-01-2020 Registration 09.30 AM ‐10.00 AM Inaugural Session Invocation – Tamil Thai Vazhthu Lighting of Kuthu Vilakku Welcome Address Dr. R.Babu Janarthanam Convenor, Associate Professor, Department of Biotechnology Thiruvalluvar University, Vellore – 632 115 10.00AM – 11.00 AM Inaugural address Prof. Dr. S.Thamarai Selvi Vice Chancellor Thiruvalluvar University Prof. Dr. Alaa Y Ghidan, Pharmacy School, Aqaba University of Technology, Jordan Felicitation Dr.S.Syed Shafi Registrar i/c Thiruvalluvar University Syndicate members Dr. G. Thangaraj Member-Syndicate Director i/c. (Affiliation & Admission) Dean i/c, CDC, Professor, Department of Mathematics Dr. R. Jeyaraman Member-Syndicate Director i/c, Centre for Academic Courses and Students Affairs, Professor, Department of Tamil Dr. M. Chitra Member-Syndicate Associate Professor , Head i/c, Department of Mathematics Dr. K. Sivachandrabose Member-Syndicate, Assistant Professor, Department of Zoology Dr. Ernest David Professor & Head, Department of Biotechnology Dr. S. Vijayanand, Assistant Professor of Biotechnology Department of Biotechnology Vote of Thanks: Dr. M.C.Harish, Assistant Professor, Dept. of Biotechnology
International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020)
11.00 AM ‐11.30 AM Technical Session – I Lecture –I: Bioremediation of Metal Contaminated Soils Dr. Seralathan Kamalakannan Professor, Division of Biotechnology Collage of environmental and Bioresources Chjonbuk National University - Iksan Campus,South Korea 11.30 AM - 11.45 AM Tea break Lecture –II: Photo-biodegradation of Imidacloprid - A novel approach 11.45 AM ‐ 12.15 PM Dr.P. Elumalai Post-Doctoral Fellow School of Environment, South China Normal University, University city campus, Guangzhou, Guangdong China 12.15 PM ‐ 12.45 PM Lecture- III Mitigation of Inhibitor for Microbial Influenced Corrosion of Carbon Steel X70 in Marine Environment Dr.J. Narenkumar Postdoctoral Researcher Corrosion and Protection Division, Shenyang National Laboratory for Materials Science, Northeastern University, Shenyang 110819, China 01.00 PM ‐ 01.45 PM Lunch Break Technical Session – II 01.45 PM ‐0 2.15 PM Lecture- IV: Water Pollution Control Engineering Dr.Saraschandra Naraginti Associate Professor School of Biological and Chemcial Engineering, Anhui Polytechnic University, Wuhu-Anhui Province, China 02.15 PM ‐ 02.45 PM Lecture- V: Electrochemical behavior of biochar and its effects on microbial nitrate reduction: Role of extracellular polymeric substances in extracellular electron transfer Dr. K. Sathishkumar Research Fellow Key Laboratory of Integrated Regulation and Resources Development of Shallow Lakes College of Environment Hohai University #1 Xikang Road, Nanjing City, Jiangsu Province P.R. China, 210098 02.45 PM ‐ 03.15 PM Tea Break 03.15 PM ‐ 04.30 PM Poster Session 04.30 PM ‐ 05.00 PM Cultural Program 05.00 PM – 5.30 PM Valedictory Session : Distribution of Prizes Feedback session Vote of Thanks Dr. A. Rajasekar. Assistant Professor & DBT-Ramalingaswami Fellow, Department of Biotechnology
International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020)
About the Vellore
Vellore is privileges as the “Fort City” and was rules by Pallavas, Cholas, Nayak, Maratha, Arcot Nawabs and Bijapur Sultan. The district lies between 12o15’ to 13o15’ north and 78o20’ to 79o50’ east in Tamilnadu state. Situated at the banks of Palar River, the city covers an area of 6077 sqkm with a total population of 3,936,331 as of 2011. “The Vellore”, emphasis on “Unity in diversity” by housing the Jalakanteswarar Temple, the St. John’s Church and a Mosque, of which the Jalakateswarar Temple is famous for its magnificent carvings. Also, Vellore is known for its Sepoy mutiny outbreak against British in the year 1857 for which a memorial pillar is built at Hazratmakkan Junction, Vellore. Adding to these, the other landmarks in Vellore include, Golden Temple, Amirthi Zoological Park & Government Museum.
Vellore has become one of the best educational destinations due to Christian Medical College & Hospital, VIT University, Voorhees College, Auxilium College, Thanthai Periyar Engineering College, Muthurangam Arts College & DKM College for Women. The city is also a home to serval manufactures such as Bharath Heavy Electricals Ltd, MRF Ltd, TVS Brakes Ltd, TEL & SIPCOT.
About the University Thiruvalluvar University was established at Vellore in the year 2002 under Government of Tamilnadu Act 32/2002. It has a sprawling campus of about 112.68 acres at Serkkadu near Valliamalai. The University is situated about sixteen kms away from the Vellore city in NH4 read connecting Chennai & Bangalore. The University was named after the great Tamil Saint “Thiruvalluvar” who wrote Ulagappothumari “Thirukkural”. The University was inaugurated by our Honorable Former Chief Minister SELVI. Dr. J.JAYALALAITHA on 16th October 2002 as a State University. The University has created four new departments, viz. Tamil, English, Mathematics and Biotechnology. Now, the total strength of the departments is Ten including six existing departments, viz, Zoology, Economics, Physics, Computer science, Commerce and Chemistry.
.
International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020)
About the Department of Biotechnology
The department was established in 2010. The department offers M.Sc., M.Phil.&Ph.D. courses. Since its inception, the department has witnessed a consistent rise demand from the students for the subject. The department has been aptly infused with highly innovative expertise & state-of- the-art infrastructure to pursue research & development in the thrust areas of Biotechnology. All the basic infrastructural facilities needed for conducting teaching & research programs have been developed in the department. The department is housed with smart class room facilities, well equipped student laboratories & research laboratories.
A separate instrumentation room consisting of sophisticated equipment & a seminar hall with public address system are also available. So far 8 batches of students have completed their M.Sc. degree course and several of them qualified in CSIR/UGC National level eligibility tests & are working in various research institutes, several prestigious institutions & biotech companies. The areas of research specialization in the department include Environment Biotechnology, Genomics, Proteomics, Metagenomics, Microbial Corrosion, Bioinformatics, Nano Biotechnology, Plant Molecular Biology & Bioprocess Engineering.
The department functions effectively both in administration & in disseminating knowledge with the tireless efforts of dedicated & efficient faculty members. Faculty members are actively involved in research activities with several research projects with extra-mural funds by DBT, DST-SERB, BRNS, AYUSH & UGC (2 Crores). Post Graduate students of the department regularly bring laurel to the department by grabbing University ranks. M.Phil & Ph.D. Research Scholars actively presented their research work in various International& national Conferences, Symposiums etc., &consistently got 1st prizes for best oral & poster presentations.
Biotechnology department regularly organize the International & National Conferences, Symposiums, Science Day Celebrations, Eminent scientists & researchers from abroad are being in regular basis for delivering lectures to interact & ignite the research acumen among the students. Recently the exchange of research scholars to HKBU, Hong Kong through the memorandum of understating (MoU). We made 3 MOU between, HKBU, Hong KONG, National Taiwan ocean University, Taiwan University of Teesside, UK.
International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020)
About the Seminar
International Seminar on Recent Advances in Biomedical Technology (RABT) 2020 provides the stake holders such a student. A platform to share knowledge & experience on their research findings, from all parts of the country to enrich their knowledge & share their experience by presenting & discussing various recent research findings in different arears Biomedical Technology. It includes presentation of posters& invited lectures by eminent persons in the field of life science.
Thrust Areas
❖ Bioremediation, Biodegradation & Biodeterioration
❖ Biosensors & Environmental biotechnology
❖ Analytical Biotechnology
❖ Advancement in Agro biotechnology
❖ Applied microbiology & microbial products
❖ Biofilms formation &quorum sensing
❖ Microbial Engineering Applied nanobiotechnology
International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020)
International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020)
International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020)
International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020)
DR. ERNEST DAVID Professor & Head Department of Biotechnology Thiruvalluvar University Serkkadu, Vellore, India – 632 115
31-01-2020
MESSAGE
On behalf of the Seminar Co-ordinator, I am happy to extend a very warm welcome to all the delegates attending the International Seminar “Recent Advances in Bioremdial Technology (RABT 2020)” being conducted by Department of Biotechnology, at Thiruvalluvar University, Vellore.
This Seminar with the theme is quite contemporary and it will cover various interdisciplinary areas of science such as, Microbiology, Biotechnology and many more and offer a scientific platform for all the participants to congregate and interact with subject specialists.
I extended my greetings to all participants and wish RABT 2020 every success.
(Dr.Ernest David)
International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020)
DR. R. BABUJANARTHANAM Associate Professor Department of Biotechnology THIRUVALLUVAR UNIVERSITY Serkkadu, Vellore – 632 115, India
31-01-2020
MESSAGE It gives me great pleasure to welcome you to the International seminar on “Recent Advances in Bio remedial Technology 2020” organized by the Department of Biotechnology, Thiruvalluvar University. The objective of the seminar is to share the knowledge on innovations in the field of biotechnology, environmental biotechnology, microbiology and nano biotechnology. This international seminar will definitely provide a wonderful forum for all the participants to refresh their knowledge base and explore the innovations. This seminar will strive to offer plenty of networking opportunities, providing the participants with the opportunity to meet and interact with the leading scientists and researchers, students, friends and colleagues as well as eminent professors. I hope that the seminar will be a real exposure in Biotechnology in society interface and inspire the younger generation to its glorious past and present.
I convey my best wishes for the conference a grand success.
(DR. R. BABUJANARTHANAM)
International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020)
Dr. M.C. HARISH Assistant Professor Department of Biotechnology Thiruvalluvar University Serkkadu, Vellore– 632 115
31-01-2020 MESSAGE
It gives me immense pleasure in sharing that our Department will be hosting a International Seminar on “Recent Advances in Bioremedial Technology (RABT 2020). The theme chosen is of topical interest and need of the hour throughout the globe to help cleaning up polluted environments, including soils, groundwater and marine environments with help of bacteria, fungi, algae and plant species which are capable of metabolizing, immobilizing or absorbing toxic compounds from their environment. As these novel biotransformations become better understood at ecological, biochemical, and genetic levels and new strategies are available for bioremediation. Hence this seminar will open up the are avenues of Bioremedial research and collaborations. I take this opportunity of conveying my best wishes to our Thiruvalluvar University authorities and Biotechnology department team for the fruitful and successful deliberation of this seminar
(DR. M.C. HARISH)
International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020)
Dr. S. VIJAYANAND Assistant Professor Department of Biotechnology Thiruvalluvar University (State University) Serkkadu, Vellore, India – 632 115
31-01-2020
MESSAGE
I am pleased and privileged to welcome you all to the International Seminar “Recent Advances on Bioremedial Technology – RABT 2020” to be held in 31st January 2020. The International Seminar has been planned to deliver the most recent advances in Bioremedial Technology and various interdisciplinary fields of Biotechnology by eminent scientists, academicians and industrialists of various national and international repute across the globe. The Seminar will provide platform for the delegates to refresh and update their knowledge base and to debate and deliberate on the emerging trends of biotechnology. The plenary lectures during the Seminar are planned featuring interesting sessions on diverse thrust areas of Bioremedial Technology. Apart from the plenary lectures, there will be poster presentation sessions for the delegates, which will serve as an ideal stage for them to share their research findings and to develop their scientific skills to the next level. I ma sure that the deliberations of this seminar will be an enlightening and inspiring experience for all the participants. It is noteworthy to mention that there was an overwhelming response to the seminar from the budding scientists and students across the country. The organizing committee has made all out efforts to make your participation worthwhile. I hope that all of you will enjoy the academic year. I once again extend my warm welcome and greetings to everyone.
(Dr.S.VIJAYANAND)
International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020)
DR. RAJASEKAR ARULIAH Assistant Professor & Ramalinaswami Fellow Department of Biotechnology Thiruvalluvar University (State University) Serkkadu, Vellore, India – 632 115
31-01-2020
MESSAGE
On behalf of the organizing committee, I am happy to extend a very warm welcome to all the delegates attending the International Seminar “Recent Advances in Bioremdial Technology (RABT 2020)” being conducted by Department of biotechnology, at Thiruvalluvar University, Vellore. I am indeed happy to organize this symposium and hope it will be useful for the participating delegates to learn and being enlightened in the areas of Bioremedial Technology to enrich and share the views in the meet. The International Seminar with the theme is quite modern and it will cover various interdisciplinary areas of science such as Biotechnology, Microbiology, Nano biotechnology and many more and provide a scientific platform for all the participants to congregate and interact with subject specialists. The seminar covers a number of plenary talks and oral presentations on newly emerging areas of Biotechnology. In view of wide range of topics of contemporary interest are lined up for deliberations in RABT- 2020 symposium. Biotechnology being an interdisciplinary field can play very vital role in finding solutions and providing appropriate technology to combat the menace of control and prevention of environmental pollution. More than 60 delegates from various stations within national and international are participating in RABT 2020 including academic/scientific/industrial etc. I take this opportunity to thank our Vice Chancellor Dr. S. Thamariselvi and The Registrar i/c Dr. S. Syed shafi and to all my colleagues for their unstinted help in organizing this International Seminar. I extended my greetings to all participants and wish RABT 2020 every success.
(Dr.A.Rajasekar)
International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020)
PLENARY LECTURES
International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020)
Prof. Dr. ALAA YOUSEF GHIDAN Faculty of Pharmacy Aqaba University of Technology, Aqaba, Jordan
SYNTHESIS AND CHARACHTERIZATION FOR DIFFERENT NANOMATERIALS AND ITS APPLICATION AS ANTIMICROBIAL AND ANTICANCER ACTIVITY
The synthesis of different nanomaterials of MgONps, AuNps, AgNps, FeONPs, are considered to be a successful way of synthesis by using aqueous extracts of peels, leaves, and fruit. The synthesized nanoparticles were characterized by UV-visible spectroscopy (UV-vis), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), transmission electron micros-copy (TEM) and fourier transform infrared spectros-copy (FT-IR). SEM and TEM analysis showed that the particles were spherical and the size of the particles ranged from 5 nm to 80 nm. The screening of synthesized bio-nanoparticles revealed that these na-noparticles were effective in increasing the Inhibition of different species from gram negative and grame positive bacteria as well as cytotoxic effect on different cell lines such as colone, breast and stomach cancer. From this study, it is concluded that the biosynthesized metal oxide nanoparticles had Inhibit the growth of different bacteria and cytotoxcity for those different nanoparticiles. Silver bionanoparticles synthesized were the best compared to other synthetic nanoparticles. Hence, it might be the best against harmful microorganisms, Anticancer activity as well.
Keywords: Anticancer Activity, Microbial Activity, Inhibition of bacteria, cytotoxic effect, cell lines
International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020)
Dr. SERALATHAN KAMALAKANNAN Professor, Division of Biotechnology, Collage of environmental and Bioresources, Jeonbuk National University - Iksan, South Korea.
BIOREMEDIATION OF METAL CONTAMINATED SOILS
Metals are naturally occurring throughout the Earth’s crust, but several metals, such as cadmium, chromium, copper, mercury, lead, nickel and zinc, and the metalloid arsenic are widely used in industries and agriculture, and are released into the environment. Bioaccumulation and biomagnifications are the major problems associated with metal contamination in the ecosystem. Conventional remediation technologies are not eco-friendly and often do not yield desirable soil properties for the reestablishment of contaminated soil. Bioremediation is a process that uses microorganisms and/or green plants or their enzymes to degrade/detoxify the toxic contaminants present in the soil. It is reported as a simple, economic and eco-friendly technology. Among the bioremediation techniques, microbial bioremediation is commonly used to remediate the metal contaminated soil. Bioaugmentation with indigenous Bacillus sp. reduced 26% of the exchangeable fraction of Pb in mine waste soil. However, bioaugmentation and subsequent bio stimulation significantly increased the immobilization rate of Pb to 36% in mine waste soil. X- ray diffraction studies confirmed the role of bacterially induced calcite precipitation in the immobilization of Pb. Bioleaching of the mine waste soil with Herbaspirillumsp. indicated that the bacteria interacted with the water soluble, strong organic, and residual fraction of metals. The Herbaspirillumsp. produces various organic acids such as lactic, malic, succinic, acetic, and citric, which could be involved in the solubilization of metals. Furthermore, the bacteria Herbaspirillumsp. was screened for plant growth promoting activity and subsequently assessed the expression rate of acdS gene in the presence of metals. The Herbaspirillumsp. were also used to enhance the phytoaccumulation rate in Zea mays L.
International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020)
Dr. P. ELUMALAI Post-Doctoral Fellow School of Environment, South China Normal University, University city campus, Guangzhou, Guangdong, China.
PHOTO-BIODEGRADATION OF IMIDACLOPRID - A NOVEL APPROACH
Imidacloprid (IMI) is a systemic nitroguanidine insecticide that belongs to the neonicotinoid family. As an agonist of the acetylcholine receptor, it attacks the insect nervous system and is extremely effective against various sucking and mining pests. IMI enters the soil during applied to agricultural field and it exhibit greater persistence with long half-life in soil. The existence of IMI in the water and soil leads to a serious threat to human health and affect the non-target aquatic organisms, the contaminant plays key role in eco-system. Outstanding threats posed IMI contamination and their environmental mitigation is a major concern, and the degradation approaches through physical, chemical and biological methods for the removal toxic substance.
This study deals a new and novel approach degradation of IMI under photo-biodegradation process. A number of pure bacterial strain isolated from sub-surface soil, that could grow by using IMI (50 mg L-1). The result showed that bacteria grew well in IMI medium and the population of strains selected three strains for further studies. IMI degrading isolated bacterial strains are PBMS-1, PBMS-2 and PBMS-3 was selected for further studies-based growth activities. The ability of bacteria (PBMS-1, PBMS-2 and PBMS-3) growth was measured (OD600) in IMI as sole carbon source medium under different colors of LEDs at regular time intervals. The observed results demonstrated that blue LEDs had higher growth rate average (OD600=0.9) while compared to other colors of LEDs lights. The average bacteria growth rate was (OD600=0.6) under other light sources. The isolates PBMS-1, PBMS-2 and PBMS-3 imidacloprid photo-biodegradation efficiency in (%) 78 ± 0.5, 72 ± 0.4 and 74 ± 0.6respectively under blue LEDs. It was found without bacteria the degradation of IMI under blue LEDs displayed 37 ± 0.3% and other light sources (red LEDs < green LEDs < white LEDs International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) Dr. NARENKUMAR JAYARAMAN, Researcher Associate, Corrosion and Protection Division, Shenyang National Laboratory for Materials Science, Northeastern University, Shenyang 110819, China. MITIGATION OF INHIBITOR FOR MICROBIAL INFLUENCED CORROSION OF CARBON STEEL X70 IN MARINE ENVIRONMENT In the current scenario, Microbial influenced corrosion (MIC) which was define as corrosion kinetic rate of a metallic material was accelerated by the microbial metabolic activity. In their suitable environment microorganism is growth and its can form aggregates of cells adhesion on the surface and frequently surrounded by a matrix of extracellular polymeric substances (EPS), which consequence in changes in surrounding environmental factors such as the salinity, oxygen concentration and acidity which might to the formation of a local oxygen concentration cell . The direct economic loss by MIC is about 300 to 500 billion dollars for year. Bacteria aided enhance in the corrosion potential and malignant alter the corrosive environment is demonstrated. The aerobic actinomyces bacterial strain, Nocardiopsisdassonvillei B17 (identify by 16S rRNA) in carbon X70 metal corrosion was evaluated by surface analysis (CLSM and SEM) and electrochemical techniques (LPR, EFM and EIS). In the presence of B17, the corrosion rate was about 15.1 mm/y, which was higher when compared to abiotic. XPS result revealed that, the presence of the Fe3O4 enhanced the cathodic reaction by the formation of protective layer on the metal surface which can be promoted with the dissolved oxygen. From the electrochemical analysis, the bacteria enhanced cathodic reaction (20.01±0.02 μA cm−2) and thus pits were observed by CLSM which may be due to cathodic depolarization. The results showed that this green corrosion inhibitor effectively reduced the corrosion rate. SEM and CLSM observations confirmed the continuation of an adsorbed protective film of green inhibitors, and thus inhibit the bacterial growth. FTIR was also employed and revealed that the adhesion of green inhibitor. Polarization studies indicated that this green inhibitor decreased the corrosion current densities by a mixed-mode mechanism (both anodic and cathodic) and exhibit 72 % corrosion inhibition efficiency. Thus, the present investigation has gained significance in reporting on a promising an alternative chemical corrosion inhibitor option to replaced. International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) Dr. SARASCHANDRA NARAGINTI Associate Professor School of Biological and Chemcial Engineering, Anhui Polytechnic University Wuhu-Anhui Province, China. WATER POLLUTION CONTROL ENGINEERING Wastewater is composed of different pollutants including toxic chemicals like Phenolic compounds, EDCs, PCPs, antibiotics, bacteria and viruses. Though various strategies have been studied for, it still remains a great challenge to degrade and detoxifies harmful chemicals employing an efficient semiconductor or bio-hybrid material. One means of attaining this objective would be preparation of new multifunctional composite materials capable of efficiently degrading and detoxifying organic pollutants via an eco-friendly process. The recalcitrant pollutants are hard to be removed with biological and other conventional methods. As an alternative, photocatalysis is a proven strategy for efficient degradation. Thus, it is expected to develop efficient materials for enhanced degradation of various pollutants. In addition, water reuse demands the toxicity assessment of the degradation products of the pollutants. Hence the phytotoxicity and biotoxicity of the products from parent pollutants photodegradation are necessary to confirm the ecofriendly detoxification of various pollutants. International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) DR. K. SATHISHKUMAR Post doctoral Research Fellow Key Laboratory of Integrated Regulation & Resources Development of Shallow Lakes, Ministry of Education, College of Environment Hohai University, Nanjing City, P.R. China, 210098. ELECTROCHEMICAL BEHAVIOR OF BIOCHAR AND ITS EFFECTS ON MICROBIAL NITRATE REDUCTION: ROLE OF EXTRACELLULAR POLYMERIC SUBSTANCES IN EXTRACELLULAR ELECTRON TRANSFER Biochar is extensively used in environmental pollutant remediation because of its diverse property, however the effect of biochar on microbial nitrate reduction and electrochemical behavior of biochar remain unknown. Also, electron transfer from the microbial cells to electron donor or acceptor have been transport across the extracellular polymeric substances (EPS), however it was unclear whether extracellular polymeric substances captured or enhance the electrons. Hence, aim of the present study is to investigate the electrochemical behavior of biochar and its effects on microbial nitrate reduction and elucidate the role of extracellular polymeric substances in extracellular electron transfer (EET). The biochar was prepared at different pyrolysis temperatures (400 °C, 500 °C and 600 °C) and their electrochemical behavior was characterized by electrochemical analysis (cyclic voltammetry, electrochemical impedance spectrum, chronoamperometry). Results demonstrated that all the biochars could donate and accept the electrons, impact of biochar on microbial nitrate reduction was studied and the results showed that biochar prepared at 400 °C significantly enhances microbial nitrate reduction process. Phenol O-H and quinone C=O surface functional groups on the biochar contributes in the overall electron exchange which accelerated the nitrate reduction. The role of EPS in EET by electrochemical analysis results reveals that outer membrane c-type cytochrome and flavin protein from the biofilm was involved in electron transfer process, and EPS act as transient media for microbial EET. Overall, present study suggested that biochar could be used as eco- friendly material for the enhancement of microbial denitrification. International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) ABSTRACTS OF THE POSTER PRESENTATION International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) MICROBIOLOGY & ENVIRONMENTAL BIOTECHNOLOGY International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) MEB/PP/01 Keywords: Biodegradation; Biosurfactant; Gas chromatography; Emulsification ROLE OF BIOSURFACTANT AND activity; Crude oil ENZYMES ON CRUDE OIL DEGRADATION WITH MEB/PP/02 ACINETOBACTER SPEICES BIO-ELECTROKINETIC (BEK) PARTHIPAN PUNNIYAKOTTIa, REMEDIATION OF HEAVY METALS RAJASEKAR ARULIAHb, SUBRAMANIA IN TANNERY EFFLUENT ANGAIAHa* CONTAMINATED SOIL USING ALKALOPHILIC BACTERIA aElectro-Materials Research Laboratory, Centre for Nanoscience and Technology, SARANKUMAR RAJA KUMARESAN 1, Pondicherry University, Puducherry- RAJASEKAR ARULIAH *1 605014, India. Environmental Molecular Microbiology bEnvironmental Molecular Microbiology Research Laboratory, Department of Research Laboratory, Department of Biotechnology, Thiruvalluvar University, Biotechnology, Thiruvalluvar University, Serkkadu, Vellore, 632115, Tamilnadu, India Vellore – 632115, India. Author email: [email protected] *Corresponding author: A. Subramania *Corresponding authors email: Email: [email protected] [email protected] Currently, the bio-remediation of aquatic The present investigation deals with environments contaminated by crude oil is a remediation of industries pollutants toxic hot challenge in environmental safety heavy metals to remove contaminated soil by science. The present study focuses on the electrokinetic (EK) and bioelectrokinetic identification of bio-surfactant (BS) and (BEK) processes. The obtained results have laccase-producing bacteria and their effective found to support EK and BEK integrated role in the biodegradation of crude oil. We system as an effective remediation of observed Acinetobacter baumannii strain electrokinetic remediation (EK) was showed their optimal development at pH 7 maximum removal percentage results as 55% and temperature 40°C. A. baumannii strain Cr, 48.21% Cu, 49.13% Pb and 52% Zn and produced about 4.68 g/l of BS respectively. the similar bioelectrokinetic (BEK) Gas chromatography confirmed that BS was experiment heavy metal removal percentage mainly composed by fatty acids. of 90.34% Cr, 89.12% Cu, 85.45% Pb and Furthermore, the role of laccase on 87.13% Zn in contaminated soil used a mixed biodegradation of crude oil by A. baumannii consortia alkalophilic bacteria Bacillus strain was studied. Maximum biodegradation cohnii (SR2) and Bacillus licheniformis efficiency (BE) was recorded for laccase- (SR3). The obtained results of the present mediated degradation (87%) followed by A. study showed an enhanced chromium baumannii strain (84%). Overall, our results removal and reduction rate by remediation of highlighted that bacterial laccase can be used chromium was further confirmed by FTIR for effective degradation of crude oil within and XRD studies. short time periods. Keywords: EK cell. DC power supply. Electromigration. Contaminated soil. alkalophilic bacteria. 1 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) MEB/PP/03 Totally seven dissimilar bacteria were identified among them three potent IDENTIFICATION OF POTENT biosurfactant producing bacteria AS2, AS3 BIOSURFACTANT PRODUCING and AS6 were identified that could efficiently MARINE BACTERIA AND ITS produce biosurfactant by utilizing crude oil APPLICATION IN BIOREMEDIATION components as the sole carbon source. The OF CRUDE OIL BY bacterial strains AS2, AS3 and AS6 were BIOELECTROKINETICS METHOD utilized for the Bioelectrokinetics process to ARUL PRAKASH ARUMUGAM & applied degraded crude oil after the addition RAJASEKAR ARULIAH * of bacterial culture in Zobell marine media supplemented with 2% (v/v) crude oil as the Environmental Molecular Microbiology sole carbon source. GC-MS results of the Research Laboratory, Department of biodegraded crude oil samples revealed that Biotechnology, Thiruvalluvar University, AS2, AS3, and AS6 were potentially Serkkadu, Vellore- 632 115. degraded various hydrocarbon contents from *Corresponding author Email: the crude oil. Therefore, our study concludes [email protected] (Dr. A. that the isolated bacteria can be used for Rajasekar) biosurfactant production and crude oil degradation for the Bioelectrokinetics. Recently, with the development of offshore oil and transportation industry, the Keywords: Biosurfactant: Crude oil: probability of oil spill is increasing on Biodegradation: Bioremediation. offshore oil platforms, oil tanker, and MEB/PP/04 offshore oil facilities. Thus, the problem of oil leakage and pollution in coastal waters A RSM BASED CHARACTERIZATION and ports has persisted for a long time. OF STAPHYLOCOCCUS EPIDERMIDIS Bioremediation technology was used to EVR4, A POTENT BIOSURFACTANT degrade oil spill this technology was PRODUCING MARINE BACTERIUM considered as the best way for remediation FOR DIESEL DEGRADATION- due to its low cost and eco-friendly method. ENZYME STUDIES AND However, the problems have been BIODEGRADATION PRODUCTS encountered to the field of application of VAISHNAVI JEEVANANDAM 1 & bioremediation technology due to RAJASEKAR ARULIAH 1* uncontrollable factors, such as the loss of effective strains and substantial decline in the 1Environmental Molecular Microbiology efficiency of remediation, therefore Research Laboratory, Department of biosurfactant producing bacterial strains will Biotechnology, Thiruvalluvar University, be identified for efficient degradation of Serkadu, Vellore 632115, Tamilnadu, India. crude oil. The present study aimed at the isolation of a potent biosurfactant producing *Corresponding author Email: indigenous bacteria which can be employed [email protected] (Dr. A. for crude oil remediation, also the Rajasekar) characterization of the biosurfactant, Anthropogenic activities of accidental oil produced during crude oil biodegradation. A spills is a major threat to environment till potent biosurfactant producing bacterial today. Therefore, we aim to isolate strains was isolated from a marine sample indigenous bacteria from the marine and identified by 16s rDNA gene sequencing. 2 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) sediment and evaluate their biosurfactant Biotechnology, Thiruvalluvar University, potentiality towards diesel oil degradation. Serkadu, Vellore 632115, Tamilnadu, India. Based on the growth and biosurfactant screening results, the highest biosurfactant *Corresponding author Email: producing isolate with best degradation [email protected] (Dr. A. efficiency was selected for biochemical and Rajasekar) 16S rRNA analysis. Thus, the identified Corrosion is a corroded of the metals by bacteria was named as Staphylococcus oxidation and reduction due to environmental epidermidis EVR4. Biosurfactant production factors like chemical and electrochemical was optimized using Response surface reaction. Microbiologically influenced methodology (RSM), software. Lab-induced corrosion (MIC) is a serious issue of current degradation studies were carried out using days. It is caused by microbes which produce Bushnell Haas medium with sterile diesel as a biofilm due to the attachment of a sole carbon source. The role of the bacterial Extracellular polymeric substances (EPS) dehydrogenase, catalase, cytochrome C were secreted on the metal surface. Cooling tower assayed for diesel degradation. Analysis by water systems (CTWSs) are an internal Gas chromatography–mass spectrometry source of tannery industries and it's used to (GC-MS) and Fourier-transform infrared remove and evaporate the wastewater. In (FTIR) spectroscopy, 100% degradation of CTWSs, more corrosion caused by microbes Nonane (C9) to Tricosane (C23), 85% of in different environmental conditions such as Pentacosane and 47% of Octacosane along high humidity, pH, Chloride and sulphite with other most probable degradation content. In the present study, products. Thus, the marine strain, EVR4 was Microbiologically influenced corrosion found be show astonishing potentiality to (MIC) was inhibited through Green inhibitor degrade diesel with a maximum degradation (Artemisia pallens) in Mild steel 1010 and efficiency of 96% within 4 d, thus confirming microbes can be isolated from cooling tower the synergistic role of both biosurfactant and water. Bacillus and Pseudomonas species enzymes. Therefore, this indigenous strain microbes are used for corrosion studies. can be considered to employ as an efficient These studies were analyzed by Colony biological agent to remediate to oil spill sites. Morphology, 16S rDNA sequencing, Keywords: Biodegradation; FTIR; GC-MS; Minimal Inhibition Concentration, Biofilm Diesel; Hydrocarbon degradation; Marine assay and Weight Loss measurements (WL). sediment Future analysis Electrochemical Measurements, surface analysis (XRD (X- MEB/PP/05 ray Diffraction Spectroscopy) SEM (Scanning Electron Microscope), and FTIR APPLICATION OF NATURAL Fourier transform infra-red spectroscopy) PRODUCTS FOR INHIBITING were done under various incubation periods BIOFILM FORMATION ON CONTROL up to 28 days. The experiment OF BIOCORROSION IN VARIOUS of Bacillus and Pseudomonas forms a thick ENGINEERING MATERIALS layer biofilm on the MS metal surface at the KOKILARAMANI SEENIVASAN1 & end of the incubation period and the WL RAJASEKAR ARULIAH1* measurement massively increased to 560 mg at 28 days when compared to the initial 1 Environmental Molecular Microbiology weight (280 ± 2 mg). The optimum 50 ppm Research Laboratory, Department of of Artemisia pallens was observed to be an 3 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) effective corrosion inhibitor. Significantly, (MIC) at 10 mg/mL. Based on this, a hybrid this research the Biofilm assay of Artemisia matrix of n-ZVI and PVA was employed pallens inhibiting biofilm formation, thereby against the microbial induced corrosion in reducing the corrosion of MS in cooling cooling tower water containing system I to tower water systems. IV, along with control. The results of the study were supported with various Keywords: Microbiologically Influenced characterizations such as, Weight loss (WL) Corrosion (MIC), Cooling Tower Water analysis, Fourier transform infrared Systems (CTWSs), Mild Steel spectroscopy (FTIR) and electrochemical 1010, Bacillus and Pseudomonas, Artemisia impedance spectroscopy (EIS). WL results pallens. showed a reduced rate of 0.08 mm/y MEB/PP/06 corrosion in n-ZVI and PVA system than other systems. FTIR results too confirmed the ANTIBACTERIAL EFFECT OF n- presence of n-ZVI and PVA, that acted as a ZEROVALENT IRON AND protective film against corrosion in Al 1100. POLYVINYL-ALGINATE HYBRID The EIS studies showed higher charge MATRIX ON MICROBIAL INDUCED transfer resistance (Rct) of 14.53 Ω and CORROSION solution resistance of 3.8 Ω. Thus, the ANANTHASELVAM AZHAGESAN 1, obtained results suggested that this novel idea SELVI ADIKESAVAN 2, & of hybrid matrix would certainly act as anti- CHANDRASEKARAN NATRAJAN1*, corrosive film in cooling tower system. 2 RAJASEKAR ARULIAH * MEB/PP/07 1*Centre for Nanobiotechnology, VIT EFFECTS OF TANNERY EFFLUENTS University, Vellore -632014, Tamilnadu. ON BIOCHEMICAL GROWTH 2*Environmental Molecular Microbiology PARAMETERS OF SOLANUM Research Laboratory, Department of LYCOPERSICUM. L (TOMATO) Biotechnology, Thiruvalluvar University, Serkadu, Vellore 632115, Tamilnadu. BALUSAMY. G1, RAJASEKAR *1 Corresponding authors: Email: 1Environmental Molecular Microbiology [email protected]*; Research Laboratory, Department of [email protected]* Biotechnology,Thiruvalluvar University, Serkkadu – 632 115, Vellore, Tamil Nadu, The present study investigates on India. antimicrobial activity of n-zerovalent iron (n- ZVI) and polyvinyl-alginate (PVA) hybrid This experimentation was carried out the matrix against microbial induced corrosion effect of tannery effluent on seed germination on Aluminum, Al 1100 metal. The study was of Solanum lycopersicum. L (Tomato) seeds conducted against corrosion inducing under the tannery spiked soil condition. The bacteria, Terribacillus aidingensis (EN3) treatments were made by mixing measured isolated from cooling tower water system. amount of tannery effluents in ground water The isolate was subjected to molecular i.e.5%,10%,15%,20%,25%, and 100% identification and the accession number of concentrations on seed germination of KR183874 was obtained from NCBI Solanum lycopersicum. L (Tomato). The seed GenBank. The antibacterial results of n-ZVI germination experiments were carried out in showed a minimal inhibitory concentration completely randomized design (CRD) with six effluent concentrations (treatments), each 4 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) experiment carried out in triplicates. The *Corresponding author Email: seeds were grown in different effluent [email protected] (Dr. A. concentrations and the effects of different Rajasekar) concentrations of effluents were compared to that of ground water (control). After the The aim of this work was to study about the experiments, 80th day, the seedlings were chromium naturally present in the water and collected and growth parameters like soil for the removal of CHROMIUM (VI) by germination percentage, shoot length, using Absorption process ions contained in number of leaves and auxiliary buds were aqueous solution using plant pisin and Iorn observed as decreased with higher amount of particles. The present study represents the 25%(v/v) up to 100% (v/v) concentrations. phyto based Adsoption Moringa Oleifera The same trend also observed in biochemical (Drumstick) and Ceiba Pentandra (panju parameters like protein, carbohydrate and Tree) for the rermoval of heavy metals. Batch chlorophyll content respectively. In adsorption studies were done for the conclusion, the Solanum lycopersicum. L efficiency of pH Various Conccentrations (Tomato) growth and biochemical and Contact time was investigated for the parameters were minimum effect with removal of heavy metals by using UV 20%(v/v) concentration comparing to Spectrophotmetry. Then obtained results control. As a result, the optimum were tested with FTIR for the analysis of concentration of tannery effluent functional group.XRD shows the iron contaminated soil was found to be 20%(v/v). particles in it. AAS shows the chromium The toxic chromium Cr (VI) was transferred level present in the given sample. Further the to different parts of the plant as non-toxic Cr Adsorption isotherms were tested with the (III). Finally, it was observed that the effluent Langmuir and Frendlich. RSM shows the can be used for irrigation purpose after relativity of the particle and Surface between appropriate dilution with ground water. them. The interaction and the nature between polymer plant gum using chromium has been Keywords: leather tannery effluent, investigated. Optical density value of various Solanum lycopersicum. L, biochemical concentrations in more ever percentage in ppm conversion. Fe¯ particles dissolve in the MEB/PP/08 gum substances. Desorption study was BIO DERIVATIVE POLYMER FOR carried out for the Moringa Olifera is removal ABSORBTION STUDY USING of 62% in it and the Other plant pisin Ceiba CHROMIUM Pentandra is 50%. aVINOTH KUMAR PM, aDHANDAPANI Keywords: Adsorption, Rsm, Gel, Ftir, P, bJANANI M, bMEENA B & Desorption. a RAJASEKAR ARULIAH* MEB/PP/09 aEnvironmental Molecular Microbiology GEL BASED PHYTO-ADSORBTIVE Research Laboratory, Department of REMOVAL OF CHROMIUM FROM Biotechnology, Thiruvalluvar University, TANNERY EFFLUENT Serkadu, Vellore 632115, Tamilnadu, India. CONTAMINATED WATER bK.M.G College of Arts and Science, VINOTH KUMAR PALUR Gudiyattam. MANOHARAN, SELVI ADIKESAVAN & RAJASEKAR ARULIAH* 5 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) Environmental Molecular Microbiology Drumstick gum of 60% and Neem gum of Research Laboratory, Department of 49% respectively. The obtained data were Biotechnology, Thiruvalluvar University, fitted with Langmuir Isotherm which showed Serkadu, Vellore 632115, Tamilnadu, India. the perfect fit of R2 value of 0.999 for almost all the plant gums. The effective removal can *Corresponding author Email: be attributed to the electrostatic interaction [email protected] (Dr. A. between the pollutant and the Phyto Rajasekar) adsorbent. Thus, the Phyto-adsorbents are Heavy metals exist as natural elements of the believed to serve as a cheap and potential earth's crust, and can also be greatly adsorbent for heavy metal removal from contributed by various anthropogenic Industrial effluents. activities. Owing to their nature of Keywords: Adsorption, Gel, Plant, AAS, degradation resistant, they tend to Isotherms. bioaccumulate and biomagnify in the environment. The present study represents MEB/PP/10 the Phyto adsorption-based removal of heavy metal, namely chromium using gel-based SYNTHESIS AND plant products. Moringa oleifera (Drumstick) CHARACTERIZATION OF BIOGENIC plant gum, Azadirachta indica (Neem), MANGANESE OXIDE Cocos nucifera (Coconut tree) and Prunus 1ANITHA. J & 1SANGEETHA Dulcis (Badam plant) gum were chosen as SUBRAMANIAN* Phyto sorbent. Batch adsorption studies were 1 conducted to determine the effect of various Department of Biotechnology, School of Bio parameters such as, pH, adsorbent dosage, Science and Technology, VIT, Vellore- contact time and initial heavy metal 632014. concentration. The efficiency of the Bioremediation is one of the most acceptable adsorbent was found to improve after alkali technique and comparatively more pre-treatment. The obtained results were advantageous than conventional method. tested with various isotherms and found to Microbe-metals interaction is explored for show best fit with Langmuir isotherm. The the synthesis of biogenic metal currently have adsorbent samples were subjected to FT-IR wide application as adsorbent or catalyst. analysis to confirm the involvement of active Manganese oxidizing bacteria (Bacillus functional groups, namely, carboxylic, mycoides) was isolated from Pichavaram hydroxyl, carbonyl, and amine groups during Lake, was identified and confirmed by 16S Phyto-sorption. AAS shows the relativity of rRNA sequencing. Optimization on the chromium present in the given sample of production and characterization of Tannery contaminated chemical Based waste synthesized biogenic manganese oxide were water which was confirmed by the obtained studied to further investigate for its results, it can be concluded that, the plant- environmental application. As the based adsorbents have showed effective biologically synthesized Manganese oxide precipitation of heavy metals which can be can be considered more eco-friendly than the easily separated by a simple centrifugation synthetic one, this research work is important step. Phyto Adsorption studies were from environmental perspective of conducted under optimized condition with synthesizing metal oxides. the maximum chromium removal by Badam gum of 60%, Coconut. gum of 57%, 6 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) Keywords: Biogenic metals, 16SrRNA MEB/PP/12 sequencing, Bacillusmycoides ISOLATION AND SCREENING OF MEB/PP/11 PROTEOLYTIC BACTERIA FROM BOVINE TANNERY FLESHING PRODUCTION, PARTIAL PURIFICATION AND APPLICATION K. GAYATHRI DEVI1, N. TANUSREEDR. OF PROTEASE FROM MICROCOCCUS K. PREETHI* LUTEUS *Assistant Professor, Department of E. RAHILA1, R. SHANTHAPRIYA1, A. Microbial Biotechnology, Bharathiar NISHITHA1 & D. CHARUMATHI2 University, Coimbatore 1II M.Sc., Biotechnology, Department of MSc, Department of Microbial Biotechnology, D.K.M College for Women Biotechnology, Bharathiar University, (Autonomous), Vellore-1 Coimbatore- 641046 2Assistant Professor, Department of E-Mail: [email protected], Biotechnology, D.K.M College for Women [email protected] & (Autonomous), Vellore-1 *[email protected] Protease producing bacteria was isolated Leather industry, one of the polluting from green peas. The bacterium was found industries of India at which huge amount of to be gram positive and round shaped. solid and liquid wastes are generated, Biochemical test results showed the organism emission of obnoxious smell because of is Indole (+), Methyl red (+), VP (+), Citrate degradation of proteinaceous material of skin (-), Oxidase (+), Catalase (+), Urease (+), and generation of gas such as NH3, H2S and Starch hydrolysis (+), Lipid hydrolysis (-) CO2. There is a much difficulty in proper and TSI (-). From these Biochemical test disposal of these wastes and insufficient results, the bacterium was identified as facilities in treating which leads to find new Micrococcus luteus. The bacteria was used ways, hence bioremediation of tannery solid to produce protease in basal salt media. The waste would be a proper method at which a enzyme activity and the specific activity of useful product also be generated out of waste. the crude enzyme were determined as 1.893 Tanning essentially is the process of U/ml and 16.528 U/mg respectively. The converting hides and skins into imputrescible crude enzyme was further subjected to partial substance. Tannery flesh are the major waste purification using ammonium sulphate and polluting material of the leather industry. precipitation method with different saturation Hence our research focusses on the flesh, ratio (20%, 40%, 60%). The saturation ratio which could be converted into a useful 40% was chosen has finest ratio for product. Beginning with the isolation of precipitating the crude enzyme with highest proteolytic bacteria from the tannery flesh specific activity 22.058 U/mg. The obtained samples which gave about 3 bacterial precipitate was further purified by dialysis in isolates. Skin are made up of collagen, a 0.2 M Phosphate buffer of pH 7. Studies on fibrous protein that could be degraded by our dehairing activity of protease on hide and isolated proteolytic bacteria. The research efficacy of protease on stain removal proved continues with the screening and proteolytic the potential uses of protease in dehairing activity of the isolated bacterial strains. The hide and laundry detergents. identified bacterial isolates belong to the Bacillus sp. The proteolytic activity was 7 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) checked and confirmed. Further work has to concentrations in order to identify its be continued for the species confirmation efficiency. with MALDI-TOF. Keywords: g-C3N4/ZnTiO3, Petrochemical Keywords: Tannery flesh, Proteolytic wastewater, Lemon Juice, Degradation bacteria, MALDI-TOF. MEB/PP/14 MEB/PP/13 AN EFFICIENT VISIBLE G-C3N4/ZNTIO3 PHOTOCATALYTIC DEGRADATION PHOTOCATALYST: GREEN OF ORGANIC DYE UNDER VISIBLE SYNTHESIS AND ITS APPLICATION LIGHT USING NITIO3 PREPARED TOWARDS PETROCHEMICAL USING LEMON EXTRACT WASTE DEGRADATION L. CHETAN, SELVARAJ MOHANA * SELVARAJ MOHANA ROOPAN* ROOPAN Chemistry of Heterocycles & Natural *Chemistry of Heterocycles & Natural Products Laboratory, Department of Product Research Laboratory, Department Chemistry, School of Advanced Science, of Chemistry, School of Advanced Sciences, Vellore Institute of Technology, Vellore Vellore Institute of Technology, Vellore Email: [email protected] Simple precipitation method was used to prepare the nanocomposite (NiTiO ). NiTiO Catalytic activity can be improved by doping 3 3 2+ was prepared by an eco-friendly method. TiO2 with metal ions like Zn . Doping on the Lemon juice was used as a reducing and graphitic Carbon Nitrite (g-C3N4) helps to stabilizing agent. The structure of NiTiO3 reduce energy band gaps and lessen electron- was compared and checked for better results hole pair recombination. g-C3N4/ZnTiO3 is at different calcination temperatures. X-ray prepared by simple precipitation method in diffraction, FTIR and Scanning Electron the presence of citric acid (lemon juice Microscope (SEM), EDAX, UV-Visible extract being the source). The nanoparticles spectroscopy and Zeta potential were used to were characterized by X-ray diffraction, characterize the nano sized structure. The UV Transmission Electron Microscopy, absorption peak depicts that the NiTiO3 is a Scanning Electron Microscopy, Atomic visible light photocatalyst. The lower band- Force Microscopy and UV-Vis absorption. gap value (2.03eV) of NiTiO3 renders an Lemon juice contains 1.44g/oz, which effective photocatalytic activity. Organic dye surpasses the any other citrus fruit. Acidic degradations are being carried out to check its medium is required for the preparation in photocatalytic activity. order to arrest agglomeration of nanoparticles due to surface repulsion. Nanoparticles have MEB/PP/15 also been found to act as contaminant carriers of co-existing contaminants and this ISOLATION AND IDENTIFICATION interaction has altered the toxicity of specific OF EFFICIENT FUNGUS SPECIES IN chemicals towards petrochemical waste THE PANCHAGAVYA AGAINST water. Collected petrochemical waste water PLANT PATHOGENIC BACTERIA was degraded in visible light photo reactor SAKTHISWARI ASHOKANA, using the prepared catalyst at different SATHIYARAJ SIVAJIA, SURIYAKALA GUNASEKRANA, 8 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) BABUJANARTHANAM MEB/PP/16 RANGANATHAN A* STUDIES ON UTILIZATION OF a&a* Nano and Energy Bioscience RIPENED MANILKARA ZAPOTA FRUIT Laboratory, Department of Biotechnology, PEEL FOR THE PRODUCTION OF Thiruvalluvar University, Serkkadu, Vellore- PHA USING BACILLUS CEREUS 632115, Tamil Nadu, India. Dr. K. PREETHI, KOUSHIKA.S a* Corresponding Author: Dr R Babujanarthanam, Department of Microbial Biotechnology, Bharathiar University, Coimbatore, Email: [email protected] Tamilnadu Panchagavya is an organic product which is Polyhydroxyalkanoates are a family of made by the cow products such as the cow’s biopolymers synthesized and accumulated by urine, dung, milk, curd and ghee. a wide range of microbes as reserve food Panchagavya is a biofertilizer that enhances materials. Polyhydroxyalkanoates have the growth of plants. Panchagavya act as a recently been the focus of attention as a biopesticide against the pathogenic microbes biodegradable and biocompatible substitute by the metabolite which is produced from the for conventional non-degradable plastics. microbes present in the panchagavya. It is the These polymers are produced in response to composition of microbes consists of bacteria; limitation of any essential nutrient required fungi etc., Fungus present in the panchagavya for cell growth and in the presence of a which was isolated by the spread plate generous supply of carbon. The material technique through potato dextrose agar properties of PHA resemble those of the medium. A group of fungus was isolated conventional thermoplastics used extensively from the panchagavya by following spread in day to day life. The advantages of PHA are plate method. By this isolated fungus, the their aerobic degradation in the presence of antibacterial activity will be observed against microbes within 5-6 weeks. In the present the plant pathogenic microbes. Fungus study, an attempt was made to isolate PHA present in the panchagavya that has the from Bacillus cereus MTCC 1272. The ability to inhibit the plant pathogens which bioprocess variables were optimized for will be observed by the zone of inhibition maximizing PHA production and ripened against the plant pathogen microbes. The Zapota fruit peel was used as a cheap carbon fungi which have the best ability against plant substrate and evaluated for the production of pathogens will be analyzed and identified by PHA. PHA was extracted using sodium the sequencing technique. Identification of hypochloride digestion method. Extracted fungal species take place by the 18s rRNA PHA was characterized using FTIR to find sequencing. In this study, we were focusing out the functional groups present. The PHA the fungus present in panchagavya which has production was done in fermentation medium the antibacterial activity against plant composed of fruit hydrolysate and the pathogenic bacteria. optimum conditions were standardized. Further studies can be carried out to improve Keywords: Panchagavya, Biopesticides, the biocompatibility of PHA and thus can be Fungi, Antibacterial activity, 18s rRNA turned to an ultimate alternative for synthetic sequencing plastic compounds. Keywords: Manilkara zapota, PHA, Bacillus cereus 9 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) MEB/PP/17 THE GROWTH RESPONSE OF SACCHARUM OFFICINARUM BIODEGRADATION OF (SUGARCANE) XENOBIOTICS (MONOCROTOPHOS) BY USING GUT BACTERIA ARULJOTHI A, NISHANTHRAJI R & JAYAPRAKASH K* D.M. GOWTHAM, II- M.Sc., Applied Microbiology, Department of Biotechnology, KMG College of Arts & Science, Gudiyattam Shanmuga industries arts and Science college, Thiruvannamalai 606 603 Biodegradation is the process by which organic substances are decomposed by *Corresponding author e-mail id: micro-organisms into simpler substances [email protected] such as carbon dioxide, water and ammonia. Monocrotophos (MCP) is an organo Fly ash is the product produced by burning of phosphorus pesticide widely used in India to coal. As a result, a huge disposal of fly ash protect economically important crops like which causes pollution. The recycling of fly cotton, sugarcane, groundnut, tobacco, ash has become an increasing concern in maize, rice, soybean, vegetables, etc. against recent years due to increasing landfill costs pests. It is a systemic pesticide and its action and current interest in sustainable is mainly on organs such as skin, eye and development. Use of fly ash in agriculture central nervous system. Monocrotophos, provides a possible alternative for it is safe commonly named Azodrin or Nuvacron, is an disposal to improve the soil environment and organophosphate insecticide, which in spite enhance the crop productivity. In present of ban is preferred due to its high efficacy study were collected fly ash from “Bannari against insect pests. Monocrotopho sun- amman sugars unit 4 kolundampattu, controlled application in farming has led to Tiruvannamalai. Based on that, the pot the contamination of surface and experiment carried out using sugarcane plant groundwater, causing neurotoxicity, (Saccharum officinarum) growing in genotoxicity, hyperglycaemic and different concentrations of fly ash (5%, 10%, stressogenic effects on different organisms. 20%, 30%, 40%, 50% & 100%) and labeled Microbes such as Bacillus, Pseudomonas, as T1-T7. Pot without fly ash is taken as Aspergillus, Anabaena and Nostocat 25–37 control. After 90 days of the experiment, the °C and pH 5.5–8.5 have the ability to utilize physicochemical parameters of soil and fly monocrotophos as nutrient source ash, biochemical parameters and heavy metal Biodegradation followed by photo catalytic accumulation in plants were noted. The degradation would be the most efficient and morphological changes of the plants were sustainable approach. This review focuses on noted at 30 days, 60 days and 90 days. At 30 toxicity, fate of monocrotophos in the days of growth all the plants showed almost environment and its microbial and photo equal growth. In 60 days, there was a slight catalytic degradation. difference in the growth of all the plants. After 90 days, there was an increase in MEB/PP/18 growth up to T4 (30% of fly ash) and the growth decreased in T5 (40% of fly ash) and A STUDY ON THE EFFECT OF FLY T6(50% of fly ash). All the plants possessed ASH FROM BANNARI AMMAN a single flower with greenish leaves and hair- SUGARS UNIT-4 KOLUNDAMPATTU covered steams. Every plant of all the TIRUVANNAMALAI AND THEIR ON concentrations was found without infections. 10 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) Plants grown in 100% of fly ash showed their antimicrobial activity seems to be stunted growth. Bio chemical parameters effective against common pathogens showed a gradual increase up to T4 (30% of (Staphyloccocus aureus, Bacillus subtilis, fly ash) and decreased in T5 (40% of fly ash) and Escherichia coli. and T6(50% of fly ash). Heavy metals accumulated in plants in the order of Fe > Mn Keywords: Mushrooms, Enrich Food, Agro > Cu > Zn > Cr. Except chromium all the waste and Paper waste heavy metals accumulated in increasing MEB/PP/20 concentration of fly ash plants. The growth was found to be optimum in 30% fly ash and DECOLORIZATION OF TEXTILE the result showed that at lower doses fly ash DYES BY USING HALOTOLERANT is a beneficial to plants. MARINE BACTERIA Keywords: Fly ash, Sugarcane, Saccharum MOHAMMED JAMALUDEEN A, officinarum And Growth Response VELMURUGAN C & JAYAPRAKASH K* MEB/PP/19 Department of Biotechnology, Shanmuga industries arts and science arts and science MUSHROOM CULTIVATION USING college, Tiruvannamalai- 606 601 AGROWASTE AND PAPERWASTE AND THEIR COMPARATIVE *Corresponding author e-mail id: ANALYSIS [email protected] CHANDRU S, GOKUL S, Textile industries are responsible for one of JAYAPRAKASH K* the major environmental pollution problems in the world, because they release undesirable Department of Biotechnology, Shanmuga dye effluents. Textile wastewater contains industries arts and Science college, dyes mixed with various contaminants at a Thiruvannamalai 606 603 variety of ranges. Therefore, environmental *Corresponding author e-mail id: legislation commonly obligates textile [email protected] factories to treat these effluents before discharge into the receiving Mushrooms are considered as enriched food watercourses. Textile effluents are having having high nutritive and medicinal values harsh physiochemical properties which are such as anticancer, antitumor and hardly tolerated by mesophilic antimicrobial activity. The present study microorganism and pollution the indicates the growth on two different environment. Marine microorganisms which substrates (banana leaves and paper wastes). usually thrive well in that harsh environment The comparative analysis was done based on condition. Halotolerant marine the development of mature fruiting bodies, microorganisms which tolerate high salt and moisture content, pH, nutritional values like pH can be suitable sources for bioremediation carbohydrates, amino acids, total protein, of textile effluents. Hence, in the present lipids, crude fibre, minerals (calcium), study, halotolerant marine bacteria were enzyme studies etc. The cultivated in paper screened for their potential in decolorizing waste possess high yield, good nutritional few important textile dyes. values such as more protein, less fat, moderate carbohydrate, high fibre, high Keywords: Mushrooms, Textile effluents, minerals – calcium and less energy. Also, Halotolerant and decolorization 11 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) MEB/PP/21 B.Sc. Biotechnology, D.K.M College for Women (Autonomous), Vellore. APPLIED MICROBIOLOGY AND MICROBIAL PRODUCTS (COSMETIC Email: [email protected] PRODUCT) Genetic engineering refers to techniques used S. AKILA & F. AMEEMA to manipulate the genetic composition of an organism by adding the specific grazes. The B.Sc. Microbiology, Kamban College of Arts enchancement of desired talks has and Science for Women traditionally been undertaken through Email: [email protected] considentional plant breed. To investigate and nature extent of microbial MEB/PP/24 contamination in cosmetic products. The ADVANCEMENT IN nature and high level of contamination used AGROBIOTECHNOLOGY cosmetic products and significance level of microbial contamination occur during use of M.J. MUBASIRA BANU & S. RAMANI cosmetic products and presence of pathogenic organism poses potential risk to Kamban College Of Arts And Science For health. Women,Thiruvannamalai MEB/PP/22 Email: [email protected] APPLIED MICROBIOLOGY AND Many Agricultural biotechnology projects MICROBIAL PRODUCTS focus on increasing yield, decreasing pesticides dependence and accessing more E. KAMALI SHREE DHARANI & R. challenging environment. However, most of MONIKA the crops are already been modified by selective breeding.so our idea on this topic is B.sc Biotechnology, D.K.M. College for to isolate the natural insect repellent found in women (Autonomous), Vellore-1 many plants. Create an environmentally Email: [email protected] friendly insect repellent without affecting the crop yield and quality of the plant."Instead of Microbiology referred as the Application of spraying poison, which inevitably also microorganisms in the field of science for the endangers the useful spices. We deliberately production of human beneficial products merely aggrevate the pest.""With our such as medicines, antibiotics, vaccines, approach, we are opening the door to a enzymes, biotechnological engineered fundamental change in crop protection." products and also in food technology as fermentation. Example (milk and milk MEB/PP/25 products). MEDICINALLY IMPORTANT MEB/PP/23 BIOACTIVE COMPOUNDS FROM CULTIVABLE INDIGENOUS EDIBLE ADVANCEMENT IN AGRO MUSHROOM LENTINUS BIOTECHNOLOGY TUBERREGIUM (Fr) Fr. S. RITHIKA, A. POORNIMA & S. J. MANJUNATHAN1*S. SHYMALA RUMANA. GOWRI2, G.N. KRISHNA KUMARI3 & V. KAVIYARASAN4 12 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) 1Assistant Professor, Department of Keywords: Cultivation, Biotechnology, Lentinustuberregium, Anticancer, Antimicrobial, LT-1 and LT-2. Vels University, Pallavaram, Chennai. MEB/PP/26 2Assistant Professor, Department of Botany and Plant Biotechnology, BIO REMEDIATION OF HEAVY METALS USING MICROALGAE Pacchaiyappa’s College, Chennai. FROM TANNERY EFFLUENT 3Head, Dept of Medicinal Chemistry, A.M. RAJALAKSHMI, Research Scholar, Department of Microbiology, Periyar Sri Ramachandra University Porur, University Chennai. DR.R. DHANDAPANI, Assistant Professor, 4Assistant Professor, CAS in Botany, Department of Microbiology, Periyar University University of Madras, Chennai. Many industrial areas in the world, show E.Mail.ID : [email protected] increases in generating of wastewater Lentinustuberregium an edible mushroom, nowadays. Industrialization is considered as collected from dead logs of rubber trees and the key factor for the development of mango trees of Keeriparai forest, countries in economic terms. The recognition Kanyakumari District of Western Ghats of that environmental pollution is a worldwide Tamil Nadu, India. Kaani tribes lives in the threat to public health has given rise to new forest, attributes many medicinal properties initiatives for environmental restoration for to this fungus. However, there are no studies both economic and ecological reasons. Heavy were carried out on this indigenous metal contamination due to natural and L.tuberregium. From the fruitbodies anthropogenic sources is a global harvested two medically important steroids environmental concern. Release of heavy have been isolated and identified as metal without proper treatment poses a antimicrobial compounds which exhibited significant threat to public health because of considerable zone of inhibition against all the its persistence, biomagnifications and test organisms. The compounds also accumulation in food chain. Non- exhibited a moderate anticancer activity in biodegradability and sludge production are four human cancer cell lines including the two major constraints of metal treatment. ovarian (SK-OV-3), breast (MCF-7) and Microbial metal bioremediation is an colorectal carcinoma (HCT-116) cell lines. efficient strategy due to its low cost, high Furthermore, these compounds were efficiency and ecofriendly nature. characterized and named as LT-1 and LT-2, Bioremediation is considered as one of the based on the spectroscopic data derived by safer, cleaner, cost effective and environment 1 13 employing H NMR, C NMR, DEPT, APT friendly technology for decontaminating sites and IR. Presence of these compounds in this which are contaminated with wide range of indigenous isolate is reported for the first pollutants. The term bioremediation has been time and we have received the patenting introduced to describe the process of using acceptance for this study. biological agent to remove toxic waste from environment. Microbes are very helpful to remediate the contaminated environment. 13 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) Algae are important bioremediation agents, crude oil as their sole carbon source while the and are already being used in wastewater amount of residual crude oil significantly treatment. A number of methods has been decreased. In addition, the cell-free broth developed for removal of such polluted containing biosurfactants produced by substances like precipitation, evaporation, bacterial strains significantly desorbed crude ion-exchange etc. oil in oil –polluted marine sediment. the selected bacteria might hold additional MEB/PP/27 capacity in crude oil degradation. BIOSURFACTANT – ASSIATED biosurfactant –producing bacteria therefore BIOREMEDIATION OF CRUDE OIL degrade crude oil hydrocarbon compounds, produce biosurfactants that can increase the V. VIJAYALAKSHMI, A. RAJASEKAR* emulsification of crude oil and are thus more conductive to the degradation of crude oil. Department of Biotechnology, Thiruvalluvar University, Sekkadu, Vellore 632115, MEB/PP/28 Tamilnadu. CULTURABLE CHROMATE Bioremediation technology was used to REDUCTION BACTERIA: ISOLATION, degrade oil spill. This technology was CHARACTERIZATION AND considered as the best way for remediation BIOREDUCTION due to its low cost and eco-friendly method. DR. G. SURESH*(V. INBASEKARAN, V. Crude oil and its derivatives are considered as SARGUNAN, S, MANIKANDAN) one group of the most pervasive environmental pollution in marine Department of microbiology, Srisankara arts environments. bioremediation using oil- and science college, enathur, kanchipuram, degrading bacteria has emerged as a Tamilnadu, India. promising green cleanup alternative in more The aim of the study is improving the recent used to reduce the low surface tension chromate remediation capacity of indigenous value of crude oil .the employment of bacteria isolated from chromium biosurfactant-producing and hydrocarbon- contaminated niches. Totally 5cr (VI)tolerant utilizing bacteria enhance the effectiveness of bacterial isolates from sediment sample were bioremediation by making hydrocarbons obtained. Out of 5 strains CB5 was found to bioavailable for degradation .In this study , be effective in 5cr (VI) reduction. The CB5 the best candidates of biosurfactant – strain was identified by 16srRNA sequencing producing bacteria were selected by of chromate tolerant bacterial isolate as screening of biochemical tests . the selected Bacillus sp. bacteria was bacillus sabtilis in general, these isolation species caused low surface tension Keywords: Chromate, Bioreduction values, high oil spreading and hydrocarbon emulsification (up to 65%) warranting MEB/PP/29 active degradation of hydrocarbons. FT-IR BIOFILMS FORMATION AND and LC-MS analyses indicated that the QUORAM SENSING monorhamnolipid and dirhamnolipid were commonly produced by the bacteria as potent SANIYA. Y & TANZEELA. N biosurfactants. The residual crude oil after Department of Biotechnology, Marudhar the biodegradation test was quantitated Kesari Jain College for Women, using GC –MS analysis. the bacterial utilized Vaniyambadi 14 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) Biofilms formation is a process whereby Department of Zoology, Thiruvalluvar microorganisms irreversibly attach to and University.PG.Extension Centre,Villupuram. grow on a surface and produce extracellular polymers. Email id : [email protected] Biofilm formation steps: The rate of cocoon production of adult earthworm, lampito mauritii kept within the * Initial attachment 0, 25, 50, 75 and 100 PSR (percent substrate * maturation and dispersion Ratio) media prepared from partly decomposed lotus waste with soil for 30 days Quoram sensing,biofilm modeling system were determined. The worms kept in 50, 75, implant associated infection and and 100 PSR media for 30 days showed a prevalence current treatment and therapeutic approach distribution of biofilm formation gradual increase in their weight up to 22 days antibiotic enhance. thereafter, a gradual decline until the termination of this study. However, the Antimicrobial photodynamic therapies. worms kept in the same media 50, 75 and 100 Early daignosis of biofilm formation using PSR after 30 days showed an increased value biosensors. in their body weight over their respective initial weight. On the contrary, all the worms DEGRADATION OF TNT BY BIOFILM kept in 0 and 25 PSR media showed a gradual * Toxicity decrease in their weight until the termination of this study. The worms kept in soil alone for * microbial degradation of TNT 30 days though showed, only 4 cocoons were -degradation by bacteria laid during the course of study thanks to less organic matters within the medium. The - degradation by fungi. worms in other PSR media for 30 days * preventive approaches to minimize produced relatively more cocoons. Though TNT contamination. than control, the worms in 75 PSR lotus MEB/PP/30 medium is taken into account to be an honest medium for the culture of earthworm, EFFECT OF DIFFERENT LOTUS lampito mauritii. (NELUMBO NUCIFERA) MEDIA ON THE GROWTH AND COCOON MEB/PP/31 PRODUCTION OF LAMPITO MAURITII ANTIMICROBIAL ACTIVITY OF *M. DEIVANAYAKI ESSENTIAL EXTRACT OIL OF ONION, NEEM AND BLOCK SEED OF PG and Research Department of Zoology, DERMATITIS Government College for Women (Autonomous) Kumbakonam - 612 001, S.ARUNA DEVI & Tamil Nadu, India. DR.A.BALASUBRAMANIAN* Corresponding Author: V.H.N.S.N.College, (Autonomous) [email protected](K. Jayachandran) Virudhunagar. Tamilnadu, India. 15 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) *Corresponding Author: as a result of bacterial stimulation. [email protected] The therapeutic strategy of using bacteria for treating cancer considered to be more A prevention and therapy pharmacy effective. composition for atopic dermatitis extract selected from the onion, neem and block seed "Scientific research is one of the most as an effective component. Natural exciting and rewarding of occupation ". antibiotics are products obtained from plants MEB/PP/33 which have been used in folk medicines for the treatment of various ailments. These MICROBIAL PRODUCTS extracts are antimicrobial activity against several microorganisms. An onion, neem and SWETHAV & INDHUJA R block seed are nontoxic and gives several [email protected] health benefits. The functional composition may be used for manufacturing a medicine Department of Microbiology, Auxilium composition and health function its anti- College (Autonomous), Vellore – 632006. atopic dermatitis effects. Those compositions Microbialcosmetics can become harmful to mainly against for the dandruff creating consumers if they are contaminated with microbes. It’s harmless because its extract harmful microorganisms such as bacteria and from medicinal plants. fungi. S. aureus, E. coli, Candida albicans Key words: Essential oils, extract, can affect cosmetics. SCP refers edible antimicrobial properties, dermatitis. unicellular microorganisms the biomass are protein extract from pure or mixed culture of MEB/PP/32 algae, yeast, bacteria may be used as MICROBES BASED MICROROBITICS ingredient. Probiotics are live IN CANCER THERAPY microorganisms promoted with claims that they provide health benefits when they RENUGA.S & ANJALI.S consumed generally by improving the gut. Probiotic foods like yogurt, kefir drink. Kamban College Of Arts And Sciences For Antibiotics being discovered were naturally Women produced antibiotics and were either This poster puts on view on wide study produced by fungi such as antibiotic towards bacteria's therapeutic roles in cancer penicillin or by a soil bacteria which are treatment, however there are a several antibiotics including streptomycin, bacterial strains/species have been tetracycline. Microbial plastics- discovered to possess inherent oncolytic Micrococcus, Streptococcus (Gram positive) potentials to invade and colonize solid tumors Pseudomonas (Gram negative) and two in vivo. Some reason decades has shows the species of fungi Aspergillus glaucus and bacterias therapeutic roles has aroused more Aspergilus niger. The work revealed that attentive in certain fields such as medicine many plastic degrading microbes can be and pharmaceutical research. Also the poster isolated from mangrove soil. A large number results seems to be promising and shows the of enzymes from bacteria, fungi have been intensive activation of the immune response reported to be involved in the biodegradation 16 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) of toxic organic pollutants. Bioremediation 1 Assistant professor, Dept. of Botany, Sri is a cost effective, nature friendly Kaleeawari College, Sivakasi. biotechnology that is powered by microbial The increase in industrialization leads to the enzymes. conventional solid and liquid waste MEB/PP/34 accumulation in the environment and the mankind. One of such important problem is ADVANCES OF BIOSENSORS IN the textile dye pollution to land and water. ENVIRONMENTAL Thus in the present study, the dye- BIOTECHNOLOGY decolorizing bacteria was isolated from the JENNIFER JEBA KIRUBA C & JANANI T textile effluents and it was identified morphologically and physiologically. The [email protected] decolorization process was measured with Department of Microbiology, Auxilium the help of UV spectrophotometer. Then the College (Autonomous), Vellore – 632006. biodegradation process was optimized by studying the effect of pH, temperature and Biosensors are device that detects, transmits effect of carbon source. From the results, it and records information regarding a was found that the organism Pseudomonas, physiological or biochemical change. with glucose as a sole carbon source, at pH 7 Biosensor also determines the level of toxic and at the temperature of 37̊ C, the substances including heavy metals like zinc, degradation process was very effective. arsenic, copper etc., in the field of ecology. Environmental field monitoring of air Keywords: Pseudomonas, biodegradation, pollution, waste degradation can also be effluent, accumulation. detected, before and after the process of MEB/PP/36 bioremediation. It plays a major role in the development of drugs and in agriculture for ADVANCEMENT IN the detection of pesticides. The food analysis, AGROBIOTECHNOLOGY especially the freshness of fruits and MADHUMITHA S & SINDHU S vegetables can also be detected with the help of biosensors. [email protected] MEB/PP/35 Department of Microbiology, Auxilium College (Autonomous), Vellore – 632006. BIODEGRADATION OF TEXTILE DYES USING BACTERIA ISOLATED The development of Agrobiotechnology FROM TEXTILE EFFLUENT offers unique and exciting opportunities for nations across Asia. Today plant A. R. VIJAYA LALITHA* & DR. S. JEYA biotechnology has been adopted by farmers KUMAR1 worldwide at rates never before seen by any *Assistant professor, Dept. of Microbiology, other advances in the history of agriculture. V. H. N. Senthikumara Nadar College, There are many benefits in Virudhunagar. Agrobiotechnology. They are crop productivity, nutritional value, environmental biotechnology, disease 17 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) resistance in crops. Using this technology it treat hyperlipidemia. Thus, our aim of the gives the improvement in crops production as study was to evaluate the anti-hyperlipidemic well as Biofertilizer. Many farmers use effect of Capsicum annuum fruits extract Biofertilizer for their lands. The (CAFE) on induced hyperlipidemic Sprague- Agrobiotechnology helps the farmers to yield Dawley rats. more crop production. They can prevent the Methods: The in vivo study was carried out crops from the disease. The with prior approval from IAEC, to evaluate Agrobiotechnology can improve the value of the anti-hyperlipidemic activity of CAFE on the crop. There are issues to resolve with Sprague-Dawley rats. As per the OECD-423 agricultural biotechnology. Some of them are guidelines, female nulliparous and non- challenging. The Agrobiotechnology pregnant rats were selected to evaluate the introduces many organic fertilizers for the acute toxicity of CAFE. Blood samples were land. collected for the evaluation of lipid profiles MEB/PP/37 and other hematological studies. Ex-vivo antioxidant effect and in vivo BIOTECHNOLOGICAL histopathological analysis of liver and PROSPECTION OF CAPSICUM adipose tissue and were also effectuated. ANNUUM AGAINST HYPERLIPIDEMIA Results: No mortality or any major signs of morbidity were recorded for the acute ABBAS ALAM CHOUDHURY1, RAMYA toxicity of CAFE. Both control and treated GUNASEKARAN1, J. HEMAPRIYA2, S. rats survived until the end of the treatment VIJAYANAND3 period. CAFE showed significant anti- 1Ph.D Research Scholar, Dept. of hyperlipidemic effect by improving lipid Biotechnology, Thiruvalluvar University, parameters in which decreased the levels of Vellore-632115, Tamilnadu, India Total Cholesterol (TC), Triglycerides (TG), 2 Low-Density Lipoproteins (LDL), and Very Assistant Professor, DKM College for Low-Density Lipoproteins (VLDL). Women, Vellore Furthermore, CAFE also showed an 3Assistant Professor, Bioresource improved antioxidant potential in the liver of Technology Lab, Thiruvalluvar University, hyperlipidemic rats. These hematological Vellore estimations were further supported by histopathological findings of the liver and Background: Human beings are naturally adipose tissue. gifted to a wide array of medicinal plants. Our source plant Capsicum annuum belonging to Conclusion: The results of the present study the family Solanaceae and genus Capsicum is revealed that the oral administration of CAFE used as ethnomedicine for the treatment of did not produce any severe acute toxic effect. various human ailments. Hyperlipidemia has In fact, CAFE exhibit a significant anti- afflicted humankind since antiquity. hyperlipidemic bio-efficacy. Conclusively, Applying the concept of ethnobotany, fruit these consequences insinuate that CAFE has extract of Capsicum annuum was taken into potential prophylactic and therapeutic consideration as an alternative medicine to bioefficacy against hyperlipidemia. 18 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) Keywords: Capsicum annuum, Cholesterol, compound which was active against V. Hyperlipidemia, Liver, Sprague-Dawley rats. paraheamolyticus and V. cholera strain. MIC value was in the range of 16µg/ml to 64µg/ml MEB/PP/38 against different pathogens. Separated PRODUCTION OF NOVEL antimicrobial compound was further ANTIMICROBIAL COMPOUNDS characterized using GC-MS analysis revealed FROM SEA CUCUMBER CRAB potent compound such as oils, phenol, (LISSOCARINUS ORBICULARIS) GUT triazine, isobornyl thiocyanoceatate, Pregn- ASSOCIATED PAENIBACILLUS 5en-20-one were important compounds POLYMYXA found in GC analysis. S. HARIKRISHNAN & S. MEB/PP/39 JAYALAKSHMI* BIOFILM FORMATION AND Centre of Advanced Study in Marine Biology, QUORUM SENSING Faculty of Marine Sciences, Annamalai CHAMBA MARGARET M & University, Parangipettai, Tamilnadu- PRIYAYANGA B 608502, India. [email protected] The present study deals with production of antimicrobial compounds from gut associate Department of Microbiology, Auxilium microbial sources. Different marine samples College (Autonomous), Vellore – 632006. such as fish, prawn and crab were collected Biofilms are a collective of one or more types from Pazhayar coastal area and their aerobic of microorganisms that can grow on many gut associated microbes were isolated using different surfaces. It can also comprise of any Zobell Marine Agar. Primary screening was syntrophic consortium of microbes in which done against human bacterial pathogens such cells stick to each other, cell to cell; called as Salmonella paratyphi, Vibrio cholera, quorum sensing. Microbes that form biofilms Vibrio paraheamolyticus, Aeromonas are bacteria, fungi and protists. Biofilms can hydrophila and Listeria monocytogenes. The be Adherent, Clinging or submerged. Factors most potential stain isolated from sea such as Environmental conditions, nutrients cucumber crab (Lissocarinus orbicularis) and bacterial growth affect biofilm was identified as Paenibacillus polymyxa formation. When microbes come into contact using biochemical and molecular technique with appropriate surfaces, they adhere, put (16srRNA sequencing Acc.no: MK583465). down roots, then a period of growth which The potent strain was optimized for growth leads to a creation of bulbous and complex for several physicochemical parameters such 3D structure with water channels. as pH, temperature, salinity, carbon and Measurement of Biofilm can done nitrogen. Mass scale culture was done using quantitatively using Scanning Electron 1L conical flasks with 0.75L of the medium Microscope in 24 well plates. Biofilms are and the antimicrobial compound was used in Medicine, bioremediation, food extracted from cell free supernatant industries, aquacultral and Microbial fuel separation of compound in TLC and the cells. following bio-autography study revealed a 19 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) MEB/PP/40 that electro-flotation method as eco-friendly and facile for the separation of textile dye SEPARATION OF TEXTILE DYE contents from industry wastewater effluent. ORGANIC CONTENT FROM Keywords: Textile effluent, electrochemical INDUSTRY WASTEWATER membrane cell, electro-flotation and EFFLUENT BY ELECTRO decolourization. FLOTATION METHOD MEB/PP/41 SAKTHIVEL KUMAR, MUNUSAMY RENUGOPAL & RAJASEKAR SUSTAINABLE MANAGEMENT OF ARULIAH* HAIR WASTE FROM LEATHER INDUSTRIES BY MICROBIAL Environmental Molecular Microbiology BIOREMEDIATION AND THROUGH Research Laboratory, Department of COMPOSTING – “A WORTH FROM Biotechnology, Thiruvalluvar University, WASTE” Serkadu, Vellore 632115, Tamilnadu, India. AADIL AHMED I* & FAROOK M A *Corresponding authors (RAJASEKAR ARULIAH*) Department of Biotechnology, Islamiah College (Autonomous), Vaniyambadi, TN, The environmental issues associated with the residual colours in textile effluents have Sustainable management of solid hair waste posed major challenges to the environmental obtained from leather tanneries by studying researchers as well as the textile colouration microbial biodegradation and through processors. In the present study, we composting which can effectively convert the demonstrated the separation of textile dye waste into organic biofertilizer. The leather contents from wastewater effluent by electro- industry generates substantial amount of flotation method using electrochemical solid hair waste globally, where mostly the membrane cell. The cation ion exchange recovered hair is disposed in landfills. membrane and metal oxide coated with Environmental concerns particularly in the titanium electrode were used for this study. developing countries, have led to increasing Electro-flotation of organic contents was demand to reduce the disposal of organic analyzed by FT-IR. Batch experiments wastes to landfill. It is believed that the studies were conducted in a stacking mode of potential use of tannery hair waste can be electrochemical membrane cell with various used as fertilizer due to its high nitrogen current intensities such as 0.2, 0.4, 0.6, 0.8 content. Composting has been proposed as and 1.0 A/cm2. The decolourization of the most suitable treatment for the recovered effluent wastewater was monitored at various tannery hair waste. However, keratin, which time intervals by UV-Visible spectroscopy. is the main constituent protein of hair, is Total carbon contents and COD levels of highly resistant to various chemical and wastewater effluent were drastically reduced enzymatic degradation, making the by electrochemical cell with optimum current biodegradation of hair to be slow and density at 0.6 A/cm2. UV-Visible spectra to inefficient under natural conditions. indicate that decolourization of textile dyes Composting has been widely accepted as an were achieved at 60 min. This study revealed environmentally friendly alternative way of 20 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) recycling the organic solid waste. Since green and sustainable. In the proposed study, composting relies upon an indigenous samples of Cr contaminated soil will be community of microorganisms, therefore can collected from the leather industrial effluent be used to find a biological agent capable of discharge area, Ranipet, Tamil Nadu. The degrading hair, understand their mode of contaminated soil sample will be degradation, and favourable process characterized by the physio-chemical and parameters for efficient decomposition of biological analysis. The soil sample will be hair in a composting environment. Currently, subjected to Electrokinetic treatment by there is very little information concerning applying current 20 V/ cm2. During the about the composting of hair waste globally. electrokinetic experiment, the anolyte and Thus, this work aims to address the current catholyte solution will be withdrawn at issue, in sustainable management of tannery different time periods to estimate total hair waste, by studying microorganisms chromium by the diphenly carboxicide involving in biodegradation of hair waste estimation method. Besides, sulphate and which can be used effectively in the chloride will be estimated by the standard optimizing the process of composting to procedures. The total viable bacteria will be produce biofertilizers. isolated and identified from the soil and will be used for the electrokinetic experiment. Keywords: hair waste, microorganisms, The integrated approach of EK and (Bio- bioremediation, sustainable management. Electrokinetic) will have been complete MEB/PP/42 removal of pollution from the contaminated soil. APPLICATION OF BIO- ELECTROKINETIC REMEDIATION Keywords: Bio-Electrokinetic; Integrated OF CHROMIMUM CONTAMINATED approach; Chromium; Bioremediation. SOIL MEB/PP/43 J. SACHINa, G. UMASHANKARa PADINA PAVONICA EXTRACTS AS C.Kavithaa and A. RAJASEKARb* GREEN CORROSION INHIBITOR FOR aPG Department of Microbiology, K.M.G MILD STEEL IN COOLING WATER College of Arts and Science, Gudiyattam. G.R. GAYATHRIa, R. SUGANYAa, P. bEnvironmental Molecular Microbiology THAMARAISELVIa, S. LAVANYAb & Laboratory, Department of Biotechnology, RAJASEKAR ARULIAHc* Thiruvalluvar University, Serkkadu, Vellore, aPG Department of Microbiology, K.M.G Tamil Nadu -632 115, India College of Arts and Science, Gudiyattam. *Corresponding Author Email: Tamilnadu, India. [email protected] (A. Rajasekar) b Department of Biochemistry Electrokinetic remediation is a potential Adhiparasakthi College of Arts & Science, technique for remediation of contaminated Kalavai, Ranipet, Tamilnadu, India. soil environment through removal of toxic heavy metals such as Cr, Ag, Al, Ti, and U. We should ensure/make that environment 21 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) cEnvironmental Molecular Microbiology VIMALA R.T.V1, Laboratory, Department of Biotechnology, SIVARAMAKRISHNAN. S1* Thiruvalluvar University, Serkkadu, Vellore. 1Department of Biotechnology, *Corresponding Author: Dr.A. Rajasekar, E- Bharathidasan University, Tiruchirappalli, mail: [email protected] Tamil Nadu, India 620024. In the present study, we demonstrated the On considering the critical issues in attaining antimicrobial activity and inhibition action of stringent water quality standards and not methanol extracts of Padina pavonica on creating any environmental impacts, we corrosion of mild steel in cooling water was focused for the first time the economically investigated by weight loss, potentiodynamic feasible, emerging technology known as Saf polarization, electrochemical impedance process (Self-assembly flocculating). In spectroscopy and scanning electron which, the study investigated the microscope. The methanol extract compound applicability of bioflocculant (a biopolymer- was analyzed by GC-MS and their evaluated self-assembly in nature) act as a surrogate on by antimicrobial activity against corrosive relying the removal of broad spectrum of causing microbes (Bacillus subtillis, substances and also, different techniques Pseudomonas aeruginosa and Bacillus have been utilized to ensure the removal cereus) from industry cooling water. Padina efficiency of pharmaceuticals, endocrine pavonica extract compound was observed the disturbing compounds, fluorescent good performance of antimicrobial activity components, and other organic materials. The and also inhibited the extracellular polymeric toxicological assessment of self-assembly substance (EPS) production. The inhibition bioflocculant implemented on zebra fish efficiencies obtained from weight loss were statistically correlated. This integrated experiment test was in good agreement. approach supplemented further information Further studies will be requirement for of zeta potential with particle size distribution surface analysis, XRD and Electrochemical to explain via Saf process. In this research, test. The present study to reveal that Padina the new insight has established non-toxic, pavonica extracts compound was confirmed self-assembly, biodegradable, bioflocculant as dual role for corrosion inhibition on the for effective bioremediation. mild steel and control the biofilm formation. MEB/PP/45 Keywords: Padina pavonica, Green RECENT ADVANCES AND Inhibitor, Microbial Corrosion, Mild Steel, CHALLENGES IN BIOREMEDIATION Antibacterial Activity (J. KUMAR, S . SENTHIL) K.KARUNA * MEB/PP/44 Sri krishna arts and science college, AN OVERVIEW OF ORGANIC kanchipuram, Tamil nadu, India. MATTERS IN MUNICIPAL WASTEWATER: REMOVAL VIA Recently, there has been an increase in the SELF-ASSEMBLY FLOCCULATING generation of contaminants which is a serious MECHANISM AND THE MOLECULAR issue to both public health and the LEVEL CHARACTERIZATION environment worldwide. An urgent need has 22 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) arisen to develop methods and assays to Physicochemical parameters are the easily access and degrade contaminants. significant alarming signs of water pollution. Bioremediation is a key technology that has The textile waters analyzed in the study proven to be of inestimable value for the showed elevated TSS, were samples DU1 control and management of contaminants and DU3 was recorded with 196 mg/L and with the aid of microorganisms. 584 mg/L, which is considerably high than Bioremediation includes a plethora of the permitted limit. The dyes and effluents strategies and methods that are currently were characterized by the presence of gaining more scientific and public interest predominant heavy metals such as cadmium, because of its safety and cost-effectiveness. chromium, lead, arsenic, and zinc. There are different kinds of wastes including Phytotoxicity analysis indicated all reactive domestic, agricultural and industrial wastes dyes had less significance effect on seed that should be treated in order to protect the germination and also affects the chlorophyll environment and keep it clean. In this content which directly signifies the toxic chapter, we highlight recent advances and effect of dyes, whereas all four effluents available bioremediation strategies and showed restricted germination of seeds and methodologies as a way to control and also a significant reduction in chlorophyll manage the different wastes with the use of content. Hemolytic assay implied Reactive biotechnology. Orange was the most toxic with 90% of RBC Keywords: Bioremediation, Pollutants, lysis, where effluents DU1 and DU2 showed Hazards, Bioleaching, Bioaccumulation, a total RBC lysis of around 87%. Artemia Phytoremediation, Post-Genomic, Era salina was used to determine LC50 values of Metabolic, Engineering, Synthetic Biology. reactive dyes and textile effluents. The LC50 values of the reactive dyes signified Reactive MEB/PP/46 Orange as the most toxic dye with 1.7 µg/mL HEAVY METAL DETERMINATION of dye and among effluents DU1 exhibited AND AQUATIC TOXICITY higher toxicity with LC50 of 15%. The study EVALUATION OF TEXTILE DYES concludes that reactive dyes and textile AND EFFLUENTS USING ARTEMIA effluents have intolerable traces of harmful SALINA heavy metals, where seed germination and hemolytic assay signified toxic effect dyes SWARNKUMAR. BA1 & W. JABEZ A2 and textile effluents. Artemia salina served as OSBORNE * an excellent model for assessment aquatic aBiomolecules Lab, School of Biosciences toxicity of textile dyes and dye-containing and Technology, VIT University, Vellore effluents 632014, India Keywords: Heavy metals, Reactive dyes, Email: [email protected], Textile effluents, phytotoxicity, and Artemia [email protected] salina The present study was aimed to evaluate the presence of harmful heavy metals in textile dyes and selected textile effluents. 23 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) MEB/PP/47 Due to human addiction to the plastic production, plastic waste as become has HYDROPONICS widespread and can be found in the deepest R. SURYA, S. SANTHIYA, K. RAMYA depths of the ocean and the top of the mountain. At present, most plastic bottle are Marudhar Kesari Jain College For Women, not truly recycled. Instead they are melted Vaniyambadi. and reformed into other hard plastic products. Hydroponic systems, such as the deep flow Recently, scientists have discovered few technique, nutrient film technique, bacteria that could eat and digest plastic. or aeroponic systems, are essential tools in Ideonella sakaiensis, bacteria that’s helps to plant factories. For adequate management of breakdown the PET PLASTIC. water and nutrients in the hydroponic system, Exiguobacterium could breakdown the the electrical conductivity (EC), pH, POLYSTYRENE. These bacteria secrete dissolved oxygen, and temperature should be enzymes that breakdowns and speeds up measured. Because ion concentrations in the degradation rate of the plastic. nutrient solutions change with time, resulting Further research should be directed towards in a nutrient imbalance in closed hydroponic making this process faster, sustainable and systems, real-time measurements of all cost-effective. This bioremediation to plastic nutrients are required, but such pollution will be an environmentally friendly measurements are not available due to recycling strategy in the future. technical problems. MEB/PP/49 Periodic analysis of nutrient solutions and adjustment of nutrient ratios can improve the APPLIED MICROBIOLOGY AND nutrient balance. As an advanced method, MICROBIAL PRODUCTS ion-selective electrodes and artificial neural L. SANDHIYA, A.SWETHA networks can be efficient tools for estimating the concentration of each ion. For stable crop Marudhar Kesari Jain College For Women, production, disinfection systems using Vaniyambadi. filters, heat, ozone, and ultraviolet radiation Applied microbiology is a branch that deals are required in hydroponic systems. with application of microorganisms in the MEB/PP/48 field of science. These microbial products are derived from various microscopic organisms. BIOREMEDIATION – PLASTIC Microbial products may consist of the POLLUTION PLASTIC EATING organisms themselves and the metabolites BACTERIA they produce. These microbes are applied in S. SANTHIYA SIGA the production of human beneficial products such as medicines, antibiotics, vaccines, Marudhar Kesari Jain College For Women, enzymes, biotechnological engineered Vaniyambadi. Tamilnadu, India products and also in food technology as EMAIL : [email protected] fermentation products. 24 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) The field of microbiology develops new methods for combating illness. Every day, microbiologists develop new products that kill viruses and bacteria. Biomedical research can result in findings that allow biologists a greater understanding of how and why infections progress. 25 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) NANOSCIENCE & NANOTECHNOLOGY(NSNT) International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) NSNT/PP/01 prospective biological application of our material in the biomedical field. BIO-APPROACH SYNTHESIS OF NANOSILVER IMPREGNATION ON Keywords: Nano-silver; calcium CALCIUM HYDROXYAPATITE BY hydroxyapatite; Urine; Biologically activated BIOLOGICAL ACTIVATED ammonia; Antibacterial and Cytotoxicity AMMONIA FROM URINARY WASTE NSNT/PP/02 P. DHANDAPANI & A. RAJASEKAR* SYNTHESIS OF SILVER NANO Environmental Molecular Microbiology PARTICLES BY USING BACTERIAL Research Laboratory, Department of STRAIN Biotechnology, Thiruvalluvar University, Serkkadu, Vellore 632115, Tamilnadu. K. VASANTHAKUMAR, II M.Sc., Applied Microbiology *Corresponding author Email: [email protected] (Dr. A. K.M.G College of Arts and Science, Rajasekar) Gudiyattam A novel biological approach is attempted to Biological synthesis of silver nanoparticles convert the human urinary waste into a well- using microorganisms has received profound designed bionanomaterial. In the present interest because of their potential to study, nano calcium hydroxyapatite material synthesize nanoparticles of various size, (HAp) has been synthesized shape and morphology. In the current study, employing,ureolytic bacteria as a biocatalyst the synthesis of silver nanoparticles by a that converted urea to ammonia from bacteria and fungus strain for example synthetic urine at room temperature (Pseudomonas aeruginosa, Actinomycetes, conditions. The impregnation of silver Stenotrophomonas and etc). The nanoparticles (AgNPs) on the HAp material nanoparticles were characterized by UV– surface was performed by photoreduction visible, TEM, EDAX, FT-IR and XRD method and was followed by an evaluation of spectroscopy. The nanoparticles exhibited an its antibacterial activity and MTT assay. absorption peak around 430 nm, a XRD, and FE-SEM were engaged to analyze characteristic surface plasmon resonance the synthesized materials. Analytical studies band of silver nanoparticles. The microbially revealed the morphology of the crystalline synthesized silver nanoparticles using were HAp synthesized by biologically activated found to be highly toxic to bacteria and it was ammonia as nano-orbicular structure along found that smaller silver nanoparticles with AgNPs impregnated on over it. Atomic synthesized by microbial route had a greater Absorption Spectrometers (AAS) estimated antibacterial activity when compared to their 2-7 ppm of Ag+ ion were released from the chemical moieties. And also, the Silver HAp-Ag-10 material. It was also found to be nanoparticles have been a potent an good performance of antibacterial activity antibacterial, antifungal, anti-viral and anti- against E.coli, S. aureus and Pseudomonas inflammatory agent. sp. The material–biointerface study of the NSNT/PP/03 thus synthesized nano-silver calcium HAp was found to exhibit a minimal cytotoxicity SYNTHESIS OF CERIUM OXIDE level when incorporated in MG63 NANOPARTICLES FROMDIFFERENT osteosarcoma cell lines, thus confirming the MEDICINAL PLANTS AND ITS IMPORTANCE 26 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) K. SHEELA, Research Scholar, Department 2 Advanced Functional Materials, CSIR- of Biochemistry, Sacred Heart College, Central Electrochemistry Research Institute Tirupattur (CECRI), Karikudi-630003, TN, India Nanotechnology is termed as a result of the 3 Dept of Physics & 3a Dept. of Bio- synthesis, characterization, exploration, and chemistry, Thiruvalluvar University College application of nano-sized materials in the of Arts & Science (TUCAS), Thennangur- event of science and technology. 604408, TV Malai (D.t), Thiruvalluvar Nanotechnology are recently developed University, Serkkadu, Vellore, Tamil Nadu, utilizing nanoparticles of transition metal India oxides, especially nanoceria that can switch between trivalent and tetravalent oxidation 4 Tamil Nadu State Council for Science & states of cerium. Cerium oxide nanoparticles Technology, DOTE Campus, Chennai- (CeO-NPs) may act as both an oxidant and an 600025, Tamil Nadu, India antioxidant, depending on the preparation Structural and magnetic properties of rare and particle size. A cerium oxide earth element (REE) La-doped CeFeO3 nanoparticles (nanoceria) has a wide range of (CeFe1-xLaxO3) nanoparticles have been applications in different fields, especially synthesized by simple chemical solution- biomedical division. As a major of concern, based microwave sintered process. The it has a major impact on the human health and resultant samples were systematically environment. The aim of this research is to characterized by structural, optical, address the different medicinal plants to morphological microscopic, magnetic synthesis of nanoceria by using Traditional, properties and application studies like bio- Chemical and Green Synthesis Methods medical and photocatalytic test at ambiance medicinal application of nanoceria for condition. From the X-ray structural assay, antibacterial, Toxicity, Antiradical activity, we found the average crystallite size (Dc) is and also exclusively discussed on the decreasing with a dopant and found to be Importance activity of cerium oxide varying between 9 – 25 nm. The surface Nanoparticles. morphology was observed analyzed by using NSNT/PP/04 high-resolution transmission electron microscope (HRTEM) shows uniform ENHANCED BIO-MEDICAL AND nanoparticles dispersed very nice manner. PHOTOCATALYTIC PROPERTIES OF Optical obtained results attributed that energy RARE EARTH ELEMENT DOPED band gap (Eg) values are increasing with CERIUM FERRITE NANOPARTICLES dopant altering between 2.05 - 2.61 eV. BY SIMPLE TECHNIQUE These results indorsed to study further bio- 1 2 chemical studies like human pathogenic and K. SELVAM , M. RAMESH , S. photocatalytic studies at room temperature. UVARAJAN3A, E. MANIKANDAN3*, G. 4 The metal oxide (M-O) stretching molecular MANI1, R. SRINIVASAN bonding vibrations and few functional groups 1Corresponding: [email protected]; are confirms the formation by infrared [email protected] spectra and the CeFeO3 nano-powders can be raised as a visible light-driven photocatalyst. 1 Dept. of Physics, Arignar Anna Govt. Arts College, Cheyyar-604407, T.V. Malai (D.t), Keywords: Microwave Method; Tamil Nadu (TN), India Nanoparticles; XRD; HR-TEM; Magnetic; E-coli Pathogen Test; Dye degradation. 27 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) NSNT/PP/05 to DMBA-treated hamsters suggestively prevented oncological incidences and POLYDATIN ENCAPSULATED POLY restored the status of the biochemical [LACTIC-CO-GLYCOLIC ACID] markers. It also significantly enhances the NANOFORMULATION COUNTERACT apoptotic associated and inhibited the cancer THE 7,12-DIMETHYLBENZ cell proliferative markers expression (p53, [A]ANTHRACENE MEDIATED Bax, Bcl-2, caspase 3, cyclin-D1). The EXPERIMENTAL CARCINOGENESIS present study reveals that POL-PLGA-NPs THROUGH THE INHIBITION OF has a feature of a possible candidate for nano- CELL PROLIFERATION chemo preventive, anti-lipid peroxidative and S. VIJAYALAKSHMI, ERNEST DAVID* antioxidant potential and also modulating the detoxification system which associated with Department of Biotechnology, Thiruvalluvar reduced cell proliferation and induced University, Serkadu, Vellore, Tamilnadu - apoptosis in experimental oral 632115 carcinogenesis. Email: [email protected] Keywords: polydatin; PLGA; nano In the present study, we are aimed to fabricate formulation; antioxidant; cell proliferation; the Polydatin encapsulated Poly [lactic-co- apoptosis glycolic acid] (POL-PLGA-NPs) to NSNT/PP/06 counteract the 7, 12- dimethyl benzyl anthracene (DMBA) promoted buccal pouch GREEN SYNTHESIS OF GOLD carcinogenesis in experimental animals. The NANOPARTICLES USING bio-formulated POL-PLGA-NPs were WEDELOLACTONE AND ITS characterized by dynamic light scattering ANTIDIABETIC ACTIVITIES (DLS), Fourier transform infrared (FTIR) THROUGH REGULATION OF spectroscopy, X-ray powder diffraction PI3K/AKT AND BCL-2 EXPRESSION (XRD) pattern analysis, transmission IN RIN-5F CELL LINE electron microscope (TEM) and atomic force RAMACHANDRAN VINAYAGAM1 AND microscope (AFM). In addition, the nano- ERNEST DAVID2 chemo preventive potential of POL-PLGA- NPs was assessed by scrutinizing the 1DR.RAMACHANDRAN V National Post- neoplastic incidence, analyzing the status of Doctoral Fellow, Department of lipid peroxidation, antioxidants, phase I, Biotechnology, Thiruvalluvar University, phase II detoxification status and Serkadu, Vellore-632 115, Tamilnadu, India. histopathological changes and in DMBA E.mail: [email protected]. treated animals. In golden Syrian hamsters, 2 OSCC was generated by painting with 0.5% ERNEST DAVID, PH.D, DMBA in liquid paraffin three times a week Professor & Head, Department of for a period of 14 weeks. 100% tumor Biotechnology, Thiruvalluvar University, formation was observed by high tumor Serkadu, Vellore-632 115, Tamilnadu, India. volume, tumor burden and altered level of E.mail: [email protected] biochemical status were observed in the We synthesized the gold nanoparticles DMBA painted hamsters. Intragastric (AuNPs) using wedelolactone (WDL) and administration of varying concentration of characterized them using UV-visible POL-PLGA-NPs (7.5, 15 and 30 mg/kg b.wt) spectroscopy, Fourier transform infrared 28 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) spectroscopy (FTIR), X-ray diffraction Department of Biotechnology, Thiruvalluvar (XRD), scanning electron microscopic University, Serkkadu, Vellore 632115, (SEM), transmission electron microscopic Tamilnadu, India (TEM), energy dispersive X-ray diffraction, and atomic force microscopic (AFM) studies. The present study was aimed to green The electronic spectrum exhibited an synthesis and characterization of AgNPs absorption peak at 535 nm. The FT-IR results nanoparticles from Aspergillus niger, which proved that WDL was stabilized on the was evaluated for their antioxidant, surface of AuNPs by acting as a capping or antimicrobial and anticancer activity. The reducing agent. The crystalline structure was synthesized NPs were characterized by affirmed by XRD pattern and the spherical various analytical techniques such as UV– shape of WDL-AuNPs was evidenced by VIS Spectroscopy, FT-IR, XRD, DLS, SEM, SEM, TEM, and AFM. The synthesized and TEM. It was confirmed through the UV– WDL-AuNPS were evaluated for anti- Vis spectrophotometer; corresponding peaks diabetic activity in pancreatic RIN-5F cell were identified at 420 nm. The green lines. In vitro results showed that WDL- synthesized AgNPs were characterized by AuNPs did not only improve the insulin FT-IR studies to reveal the functional group secretion affected by di-(2-ethylhexyl) attributed to the formation of AgNPs. phthalate (DEHP), but also the cell viability Morphological size of AgNPs was 80–130 in RIN5F cells. WDL-AuNPs treatment nm found through characterization by DLS, modulates the pro-apoptotic proteins and SEM, and TEM. Furthermore, the green anti-apoptotic proteins expression to prevent synthesized AgNPs showed potent the cells undergoing apoptosis in DEHP- antioxidant (ABTS and DPPH assay) exposed RIN-5F cells. The exposure of antimicrobial activity against clinical DEHP causes an increase in ROS production pathogenic bacterial strains such as and lipid peroxidation levels. The free radical Klebsiella pneumoniae, Escherichia coli, scavenging and antioxidant properties of Pseudomonas aeruginosa, and Enterobacter WDL-AuNPs increase the deleterious effect aerogenes. In addition, the green synthesized caused by DEHP. On the other side, WDL- AgNPs showed the dose-dependent AuNPs increase mRNA expressions of cytotoxicity and apoptotic features in Triple insulin-signaling proteins in RIN-5F cells. negative breast cancer. The overall findings This study concludes that WDL-AuNPs can of the study suggested that A. niger had a be successfully used to regulate the potential for the biosynthesis of AgNPs as an expression of Bcl-2 family proteins, reduce alternative biomaterial for future therapeutic lipid peroxidation, and to improve the application as an antioxidant, antimicrobial secretion of antioxidants and insulin through and anticancer activity. the GLUT2 pathway in RIN-5F cell lines. Keywords: Aspergillus niger, AgNps, NSNT/PP/07 Antimicrobial, Antioxidant, MD-MBA231 BIOFABRICATION OF SILVER NSNT/PP/08 NANOPARTICLES FROM MATHEMATICAL MODELS ASPERGILLUS NIGER: A POTENTIAL APPROACH FOR OPTIMIZATION BIOMEDICAL APPLICATION AND VALIDATION OF BISPHENOL-A SANTHOSHKUMAR MURALI, ERNEST TREATMENT, USING SURFACE DAVID ENGINEERED MULTI-WALLED CARBON NANOTUBES 29 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) MOHAMMED HABEEB AHMED, VISCOSA USING SILVER SANGEETHA SUBRAMANIAN* NANOPARTICLES Department of Biotechnology, Vellore SURESH YARRAPPAGAARI1, Institute of Technology, Vellore. LAVANYA THOPIREDDY2, RAJESWARA REDDY SADDALA1, * Email id:[email protected] 1Division of Animal Biotechnology, In this, present study, manganese oxide Department of Biotechnology, School of nanoparticles were surface bonded to multi- Herbal studies and Nature Sciences, walled carbon nanotube to develop an Dravidian University, Kuppam-517 426, adsorptive-oxidative composite for Andhra Pradesh, India Bisphenol-A treatment. Characterization revealed the higher sustainable and beneficial 2Department of Zoology, Government properties of the composite for wastewater Degree College, Kuppam-517 426, Andhra treatment. pH 4, initial concentration 50ppm, Pradesh, India contact time 60min and adsorbent dosage 1 g/L were found to be optimal removal *Correspondence: Dr. Rajeswara Reddy conditions and up to 100% removal with 2 Saddala Assistant Professor, Division of g/L of dosage. Adsorptive process was found Ethnopharmacology, Department of be following pseudo-second-order reaction Biotechnology, Dravidian University, and well explained by Freundlich isotherm Kuppam – 517 426, model. XPS analysis helped in evaluating the Email :[email protected] oxidative property of composite, confirming the oxidation of Bisphenol-A molecules. The role of silver nanoparticles (AgNps) is an Response surface methodology based central attractive proposition for advancing modern composite design was applied for optimizing diabetes therapies and applied science. Stable the parameters, which exhibited the process AgNps were synthesized using aqueous to be satisfactorily fitting with quadratic extract from Cleome viscosa. The size, shape model. Analysis and validation of the and elemental analysis were carried out using parameters was performed by artificial neural UV–Visible spectroscopy, scanning electron network model. Additionally, Composite was microscopy with energy-dispersive X-ray subjected to synthetic pharmaceutical spectroscopy (SEM–EDX), Fourier effluent spiked with Bisphenol-A to evaluate transform infrared (FTIR), X-ray diffraction for real time treatment efficiency, which (XRD) and Transmission electron resulted up to 61% TOC removal with given microscope (TEM). Inhibition of α- 2 g/L dosage in 60mins. glucosidase and α-amylase enzymes retards the rate of carbohydrate digestion, thereby Keywords: Bisphenol-A, Multi-walled provides an alternative and a less evasive carbon nanotube, Adsorptive-oxidative strategy of reducing postprandial composite, Central composite design, hyperglycemia in diabetic patients. The Artificial neural network, Pharmaceutical AgNps derived from Cleome viscosa, effluent respectively displayed an inhibitory effect at NSNT/PP/09 78.14% in α-glucosidase and 46.92% in α- amylase respectively, as a result, the bio- ENHANCING ANTIDIABETIC derived AgNps displayed good antidiabetic PERFORMANCE OF CLEOME activity. 30 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) Keywords: Cleome viscosa, silver nanoparticles could be employed for tertiary nanoparticles, SEM-EDX, FT-IR, treatment of microbial contaminated water. antidiabetic activity Keyword: Antibiotic resistance, NSNT/PP/10 Antibacterial, Antioxidant, Achromobacter agilis, E.coli, Staphylococcus aureus ANTIMICROBIAL BIOGENIC SELENIUM NANOPARTICLE FROM NSNT/PP/11 ACHROMOBACTER AGILIS AGAINST ESCHERICHIA COLI AND EVALUATION OF ANTIBACTERIAL, STAPHYLOCOCCUS AUREUS FREE RADICAL SCAVENGING AND ANTICANCER ACTIVITY OF JAYANTHI.V, SANGEETHA SYNTHESIZED SILVER SUBRAMANIAN* NANOPARTICLES School of Bioscience and Technology, VIT SURIYAKALA GUNASEKARANA, University, Vellore SATHIYARAJ SIVAJIA, DHANESH GANDHI ARUMUGAMA, [email protected] BABUJANARTHANAM Drug-resistant bacteria possess higher health RANGANATHANA* threats to human. According to WHO, a&a* Nano and Energy Bioscience worldwide 70 lakh people die per year due to Laboratory, Department of Biotechnology, disease and illness caused by antibiotic Thiruvalluvar University, Serkkadu, Vellore- resistance bacteria. Secondary treated waste 632115, Tamil Nadu, India. water contains large number of pathogenic bacteria that are drug resistant. Escherichia a* Corresponding Author: Dr R coli and staphylococcus aureus are the Babujanarthanam, common microbial contaminants present in the secondary treated wastewater causing Email: [email protected] serious health impact on human. The The present study aimed to study plant available wastewater treatment techniques mediated genesis of silver nanoparticles from are not effective against the treatment of Plumeria pudica jacq. flower extract. UV- antibiotic resistant bacterial strains. visible spectrum showed peak at 434 nm Nanotechnology based approach is one of the which confirming the formation of silver reliable sources for treating multi-drug nanoparticles and Fourier transform infra-red resistant bacteria. Selenium nanoparticle spectrum further confirmed the involvement posse’s higher inhibition efficiency towards of biological components in nanoparticle both Gram positive and Gram-negative synthesis. X-Ray Diffraction, Transmission bacteria. In present study, biogenic selenium Electron Microscopy and Energy-dispersive nanoparticles were synthesized X-ray spectroscopy showed Crystalline extracellularly using Achromobacter agilis nature, spherical morphology and elemental and found to be spherical in shape with size composition of the synthesized nanoparticles ~13.11nm and zeta potential of -45.33mV. (PP-AgNPs). Antibacterial activity of PP- Synthesized nanoparticles demonstrated AgNPs showed highest inhibition against E. higher antimicrobial and anti-oxidant coli (17.33 ±0.26 mm) at 40 μl concentration. activity. Up to 99% growth was inhibited in Free radical scavenging was done by 2,2- E.coli and Staphylococcus aureus (~106 diphenyl-1-picrylhydrazyl, Hydroxyl, CFU/ml). Obtained results indicate that these superoxide and 2,2'-azino-bis (3- 31 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) ethylbenzothiazoline-6-sulphonic acid) suspension. But the result was opposite in assays, the inhibitory concentration (IC50) case of copper solution. The stability of CuO was calculated. PP-AgNPs showed NPs in Mnt clay was more than the stability significant cytotoxicity against HepG2 cell without support. Photodegradation activity line in a dose dependent manner and induces was evaluated by the discoloration of ROS production and apoptosis by time methylene blue aqueous solution under UV dependent manner. Based on our findings, irradiation. Leaching tests confirmed strong PP-AgNPs exhibited biomedical properties interaction between Montmorillonite matrix and could be used against free radicals, and CuO nanoparticles, and, therefore, high cancer and other bacterial infectious diseases. stability of prepared nanocomposites. Further, Biological activity results showed Keywords: Plumeria pudica jacq., Silver that CuO NPs can be used as effective growth nanoparticles, HepG2 cell line, Apoptosis inhibitors in different biological systems. NSNT/PP/12 Keywords: CuO nanoparticles, BIOLOGICAL AND Montmorillonite, biological, Photocatalytic PHOTOCATALYTIC REMEDIATION activity, Methylene blue. OF CUO NANOPARTICLES NSNT/PP/13 DECORATED MONTMORILONITE CLAY MINERAL ITS EFFECTIVE PHOTOCATALYTIC DEGRADATION OF CATALYTIC PERFORMANCE CONGO RED USING ECO-FRIENDLY ZINC OXIDE NANOPARTICLES G. MADHUMITHA J. FOWSIYA, G. MADHUMITHA* Chemistry of Heterocycles & Natural Product Research Laboratory, Department of Chemistry of Heterocycles& Natural Product Chemistry, School of Advanced Science, Laboratory, Department of Chemistry, Vellore Institute of Technology, Vellore- 632014, India. School of Advanced Science, Vellore Institute of Technology, Vellore 632 014, Tamil Nadu, Email: [email protected] India The CuO/Montmorillonite nanocomposite, *Email: [email protected]; was formed from CuO/Mnt clay suspension. [email protected]; CuSO4 and Carissa edulis extract were used as a copper oxide precursor and reducing The green methods are being currently used agent, respectively. The nanocomposite was for synthesis of metal oxide nanoparticles. In characterized by x-ray diffraction (XRD), this study, latex of Calotropis gigantea (C. Fourier transform infrared spectroscopy gigantea) has been used for the first time as a (FTIR), transmission electron microscopy reducing material for the synthesis of ZnO (TEM), and UV–vis spectroscopy. The XRD nanoparticles (ZnO NPs). The showed the intercalation of CuO characterization of ZnO NPs was carried out nanoparticles (CuO NPs) into the clay using UV-Vis spectroscopy, XRD, SEM and interlayer space. TheUV–vis indicated a TEM analysis. Further the green synthesized strong surface plasmon resonance (SPR) ZnO NPs was used to degrade Congo red dye absorption band in the UV region, resulting which is toxic to environment. The ZnO NPs from CuO NPs. The intensity of the SPR was showed a significant degradation of band increased with time in the Mnt Congo red and it can be lead to further research on C. gigantea latex and ZnO NPs. 32 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) Keywords: C. gigantean, latex, ZnO different parameters such as amount of nanoparticles, Congo red, Photodegradation. nanocomposite, concentration of the dye and pH of the solution were studied. The results NSNT/PP/14 showed that modification of Ag/Mt BIO-SYNTHESIS OF SILVER nanocomposite increased the percentage of NANOPARTICLE MODIFIED discoloration amaranth dye and degradation MONTMORILLONITE CLAY AND ITS of ciprofloxacin. Mt matrix showed an PHOTOCATALYTIC DEGRADATION important role in the reduction of recombination of electron-hole in T. CHELLAPANDI, G. MADHUMITHA* nanocomposite. Chemistry of Heterocycles & Natural Keywords: Carissa edulis fruit extract, Ag, Product Research Laboratory, Department Montmorillonite clay, photocatalytic of Chemistry, School of Advanced Science, degradation, Amaranth dye and Anti-biotic Vellore Institute of Technology, Vellore- drug Ciprofloxacin. 632014, India. NSNT/PP/15 Email: [email protected] ANTIBACTERIAL AND FREE The silver modified montmorillonite clay RADICAL SCAVENGING ACTIVITY (Ag/Mt) nanocomposite was prepared using OF GREEN SYNTHESISED SILVER Carissa edulis fruit extract. To improve the NANOPARTICLES photocatalytic properties of Ag-Mt nanocomposite, silver metal nanoparticles URMILA GOPAL PARANTHAMANA, A was deposited over nanocomposite. AgNO3 SURIYAKALA GUNASEKRAN , and Carissa edulis extract were used as a SATHIYARAJ SIVAJIA, silver pre-cursor and reducing agent, BABUJANARTHANAM respectively. The nanocomposite was RANGANATHANA* characterized by X-ray diffraction (XRD), a&a* Nano and Energy Bioscience Fourier transform infrared spectroscopy Laboratory, Department of Biotechnology, (FTIR), Surface analysis BET, Zeta potential, Thiruvalluvar University, Serkkadu, Vellore- Scanning electron microscopy (SEM), 632115, Tamil Nadu, India. transmission electron microscopy (TEM), and ultraviolet-visible diffuse reflectance a* Corresponding Author: Dr R spectroscopy (DRS). The powder X-ray Babujanarthanam, diffraction showed that Ag nanoparticles located on the surface Mt layers. The diffuse Email: [email protected] reflectance spectra of nanocomposite Green synthesised of silver nanoparticles indicated a strong surface plasmon resonance have been widely used in the field of (SPR) absorption band in the visible region, medicine. The present study involves green resulting from metallic Ag nanoparticles. route synthesis of silver nanoparticles using TEM image demonstrated the presence of Pithecellobium dulce peel extract as a silver nanoparticles with an average size of 4- reducing agent. Synthesized silver 8 nm over both Mt. The photocatalytic nanoparticles were preliminary observed by activity of nanocomposite was studied for colour changes from pale yellow to dark destructive reaction amaranth food product brown. Then the formation of silver dye and Anti-biotic drug ciprofloxacin under nanoparticles was characterized by UV– UV-visible light. In addition, the effects of Visible spectroscopy which shows high peak 33 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) at 407 nm. The Fourier transform infra-red "Nanorobot is an excellent tool for future spectrum revealed that the functional groups medicine." of peel extract which is used for the capping of synthesized silver nanoparticles. NSNT/PP/17 Crystalline nature and spherical shape were PHOTOCATALYTIC DEGRADATION noted using X-Ray Diffraction and AND KINETIC STUDIES OF NON- Transmission Electron Microscopy. Through CONVENTIONAL ECOFRIENDLY Dynamic Light Scattering analysis, the average size of silver nanoparticles was SYNTHESIZED CUO found to be 78.3 nm. The Antibacterial NANOPARTICLES activity of synthesised silver nanoparticles D. DEVI PRIYA, S. MOHANA ROOPAN* will be evaluated. The free radical scavenging activity of synthesized silver Chemistry of Heterocycles & Natural nanoparticles will be analysed. It is Product Research Laboratory, Department concluded that the green synthesis of silver of Chemistry, School of Advanced Science, nanoparticles is very fast, cost effective & Vellore Institute of Technology, Vellore, 632 eco-friendly and does not cause any side 014, Tamilnadu, India effects to humans. E-mail addresses: Keywords: Green synthesis, Silver [email protected] (S.M. nanoparticles, TEM, antibacterial, free Roopan). radicals scavenging The advancement in the field of green NSNT/PP/16 nanotechnology is bringing about immense enthusiasm towards analysts to center around NANOBOTS IN CANCER TREATMENT eco-friendly synthesis of nanoparticles. In P. BHUVANESHWARI & S. KANIMOZHI this examination, biosynthesis of copper oxide nanoparticles (CuO NPs) was finished Kamban College of Arts and Science For utilizing leaf concentrate of Senna auriculata Women, Tiruvannamalai (S. auriculata). The biosynthesized CuO NPs This poster presents a study on different were characterized by different expository approaches employed towards cancer methods like UV-Vis spectroscopy, X-beam treatment using nano robots. A diffraction (XRD), Fourier transformation multifunctional platform based on infrared spectroscopy (FTIR), SEM nanorobots, with various types of (Scanning Electron Microscope), nanomachines will surely fight against major Transmission Electron Microscope (TEM) to diseases like cancer, HIV etc. There are distinguish its physical and morphological several other potential medical applications, properties. It was examined that the S. including repair of damaged tissue, auriculata leaf reduced copper particles into unblocking of arteries affected by plaques. CuO NPs from 8 to 10 min of response time. This include building complete replacement The consequences were observed at 280 nm of body organs. in UV-Vis range. Additionally, the XRD range demonstrated the combined CuO NPs Nowadays medical science is more and more are in crystalline nature. The TEM improving with the blessings of new investigation discovered that the ecofriendly scientific discoveries. incorporated CuO NPs are appropriate spherical shape with 28 nm size. The 34 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) photocatalytic progress of CuO NPs S.Zakira Banu , M.Santhoshkumar, Ernest indicated 97 % of degradation of Malachite David* green. Department of Biotechnology, Thiruvalluvar Keywords: Ecofriendly synthesis, Senna University, Serkkadu, Vellore 632115, auriculata (S. auriculata), CuO NPs, Dye Tamilnadu, India degradation, Kinetic studies. The present study was aimed to green NSNT/PP/18 synthesis and characterization of silver nanoparticles (AgNPs) from Gymnema GREEN SYNTHESIS AND sylvestre. The synthesised NPs were CHARACTERIZATION OF GOLD characterized by various analytical NANOPARTICLES FROM CYNODON techniques such as UV–VIS Spectroscopy, DACTYLON AND ITS FT-IR, XRD, DLS, SEM, and TEM. It was ANTIMICROBIAL ACTIVITY confirmed through the UV–Vis K. MAHALAKSHMI, ERNEST DAVID* spectrophotometer; corresponding peaks were identified at 400 nm. The green Department of Biotechnology, Thiruvalluvar synthesized AgNPs were characterized by University, Serkkadu, Vellore 632115, FT-IR studies to reveal the functional group Tamilnadu, India attributed to the formation of AgNPs. The To present study was synthesis and green synthesised AgNPs showed potent characterization of gold nanoparticles using antibacterial activity against clinical from cynodon dactylon plant extract. The pathogenic bacterial strains such as characterization of AuNPs were carried out Klebsiella pneumoniae, Escherichia coli, by ultraviolet-visible spectroscopy (UV-Vis Pseudomonas aeruginosa, and Enterobacter UV-visible spectrum of the aqueous medium aerogenes. Thus, this method can be used for containing Au nanoparticles showed a peak rapid and eco-friendly synthesis of of 517 nm. TEM analysis was performed to biocompatible silver nanoparticles examine the size and shape of the possessing Antibacterial activity suggesting biosynthesized Au nanoparticles. TEM their possible application in medical industry. analysis indicated that AuNPs nanoparticles Key Words: Silver Nanoparticle, were well dispersed and ranged between 15 Antibacterial activity, Gymnema sylvestre, to 30 nm in size. Antibacterial activity of the Escherichia coli. biosynthesized AuNPs was studied against common human pathogens such Escherichia NSNT/PP/20 E.coli Pseudomonas aeruginosa and GREEN SYNTHESIS OF ZNO Staphylococcus aureus agar well diffusion NANOPARTICLE USING THE LEAF method. EXTRACT OF CORDIA SEBESTINA NSNT/PP/19 1JAYABHARATHI GREEN SYNTHESIS AND THIRUMALAZHAGAN, AYESHA CHARACTERIZATION OF SILVER SIDDIQUA2, RAJARAM K3, NANOPARTICLES FROM GYMNEMA SHANMUGAVEL M4 & MANI U3* SYLVESTRE LEAF EXTRACT: STUDY OF ANTIBACTERIAL ACTIVITIES 1Department of Biotechnology, Stella Maris College, Chennai 35 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) 2Department of Microbiology, K.M.G. Nanobiotechnology is the application of College of Arts & Science, Gudiattam nanotechnology in biological fields. It is a scientific approach that involves materials 3Department of Biochemistry and and equipments capable of manipulating Biotechnology, CSIR-CLRI, Chennai physical as well as chemical properties of a 4Bio-Material Laboratory, CSIR-CLRI, substance at molecular levels. Biotechnology Chennai uses the knowledge and techniques of biology to manipulate molecular, genetic and Nanotechnology is the next generation cellular processes to develop products and technology that is being concentrated in the services and is used in diverse fields from recent years. Synthesis of nanoparticles has medicine to agriculture. Nanobiotechnology many approaches. In this present study, green is considered to be unique fusion of approach was used for the production of ZnO biotechnology and nanotechnology by which nanoparticles using plant extract. The plant classical microtechnology can be merged to a extract has phenolic compounds that act as molecular biological approach in real. reducing agents that reduce the ZnSO4 to Through this methodology, atomic or ZnO nanoparticals. Cordia Sebestina molecular grade machines can be made by commonly known as Geiger tree is known to mimicking of incorporating biological be used for gastrointestinal problems like machines. Nanotechnology is defined as the indigestion. Water extract was prepared from design, development and application of leaves of Cordia Sebestina and used for the materials and devices whose least functional synthesis of ZnO nanoparticle. The makeup is on a nanometer scale. synthesized nanoparticles were confirmed using UV spectroscopy, FTIR, TEM and NSNT/PP/22 XRD. The ZnO particles further used for SYNTHESIS AND various applications like antibacterial studies CHARACTERIZATION OF SILVER and photocatalytic dye degradation. Further NANOPARTICLES USING MARINE research they can also be used for direct BROWN ALGA (PADINA PAVONIA) pharmaceutical purposes as they are AND THEIR ANTIBACTERIAL biocompatible. ACTIVITY Key Words: Nanoparticles, Zno4, Cordia SATHISH VENKATESAN* & Sebestina, TEM, FTIR, dye degradation DEIVASIGAMANI BALARAMAN * NSNT/PP/21 Faculty of Marine Science CAS in Marine Biology Annamalai University Parangipettai. APPLICATIONS OF NANOBIOTECHNOLOGY The marine brown alga (Padina pavonia) extract was used for the synthesis of AgNO3 SAKALA PATRICIA & GOKULA PRIYA at room temperature. UV–visible N spectrometry study resonance at 413 nm. The Department of Microbiology, Auxilium characterization of silver nanoparticle was College (Autonomous), Vellore – 632006. carried out using Fourier transform infrared spectroscopy (FT-IR), Scanning electron [email protected] microscopy (SEM), X-ray diffraction (XRD) 36 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) and transmission electron microscope synthesised selenium nanoparticles exhibited (TEM). FT-IR measurements revealed the characteristic peak at 280nm in UV spectral possible functional groups responsible for analysis, XRD and FESEM analysis revealed reduction and stabilization of the the nanoparticles are crystalline in nature nanoparticles. X-ray diffraction analysis with average size of 20-50 nm. The FTIR showed that the particles were crystalline in spectrum showed the cultural supernatant of nature with face-centered cubic structure. strain B2 responsible for reduction of sodium TEM micrograph has shown the formation of selenite. The antibacterial activity of the Se silver nanoparticles with the size in the range NPs shows potent activity against both gram of 5–20 nm. The synthesized AgNPs have positive and gram-negative water borne test shown the best antibacterial activity against bacteria. The lowest MIC and MBC of Se human pathogens such as Escherichia coli. NPs was recorded as 8µg/ ml against The above eco-friendly synthesis procedure Salmonella typhi (NCIM 5031). Based on of AgNPs could be easily scaled up in future this we conclude that selenium nanoparticles for the industrial and therapeutic needs. could be effectively used for drinking water treatment. Keywords: Padina pavonia, Escherichia coli, AgNO3, TEM and UV–visible Key words: Fresh water bacteria, selenium spectrometry. nanoparticles, Bacillus altitudinis, antibacterial NSNT/PP/23 NSNT/PP/24 BIOSYNTHESIS AND CHARACTERISATION OF SELENIUM IMPACT OF BIOENGINEERED ZINC NANOPARTICLES AND VALIDATION OXIDE NANOPARTICLES EXPOSURE FOR ANTIBACTERIAL ACTIVITY ON PHYSIOLOGICAL AND AGAINST WATER BORNE BIOCHEMICAL CHANGES IN PATHOGENS SHALLOT (ALLIUM ASCALONICUM) ANURADHA. K., SANDHIYA. K., N. PRIYANKA AND P. ROGINI. M., SUMITHRA. S., VENKATACHALAM* THANGAMANI. V., BHARATHI. S* Plant Genetic Engineering and Molecular Department of Microbiology, Sri Sankara Biology Lab, Department of Biotechnology, Arts and Science College (Autonomous), Periyar University, Periyar Palkalai Nagar, Kanchipuram, Tamil Nadu, India Salem – 636 011, Tamil Nadu, India. In this present study, selenium reducing (*For Correspondence: bacteria were isolated from fresh water pond Email:[email protected]) in Kanchipuram. The bacterial strain B 2 Nanoparticles are minute carriers which help found to effectively reduce sodium selenite in enhanced delivery of phyco molecules to and was identified as Bacillus altitudinis treat a specific site in a short duration. based on 16S r RNA sequencing. The Seaweed was used as a fertilizer to increase selenium nanoparticles synthesised using seed germination and improved plant growth. Bacillus sp B2 was characterised by UV- The present study narrates the role of phyco spec, XRD, FTIR, FESEM and EDX. The 37 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) coated ZnONPs on plants growth utilizing plant concentrates wound up one of characteristics and biochemical changes in the potential territories of research. The shallot after 30 days of revelation. ZnONPs bioreduction of metal particle is very fast, treated plants exhibited increased shoot and promptly perform at room temperature and root growth by 132.50 % and effectively scale up. The present examination 227.30%respectively and biomass compared portrays a fast and eco-accommodating to the untreated control. Results amalgamation of AgNPs utilizing the demonstrated that the level of photosynthetic productivity and the impact of different pigment contents namely, chlorophyll a procedure factors in the biosynthesis of 600.90%, chlorophyll b 512.01 % and AgNPs broke down incorporate repetitive carotenoids 309.96% increased by ZnONPs focus, temperature and time. Green synthesis exposed plants over control. Interestingly, the of silver nanoparticles using the aqueous activity of antioxidative enzymes viz extract of Alternanthera sessilis under superoxide dismutase (SOD), peroxidase various experimental conditions. The (POX) and catalase (CAT) were significantly aqueous extract of Alternanthera sessilis increased up to 200mg NPs treated plants showed significant potential for the quick than the control. Results suggested that reduction of silver ions. The synthesized engineered phycomolecule loaded ZnONPs silver nanoparticles were characterized with could be used as nanofertilizer to increase UV-visible absorption spectrophotometer, crop productivity. XRD, SEM, and FTIR analysis. The average crystallite size as calculated from x-ray Keywords: Antioxidative system, Crop diffraction studies and SEM analysis was yield, Growth responses, Nanofertilizer, found to be less than 100 nm.The AgNp Phytonanotechnology, Zinc oxide sample showed better antioxidant activity in H2O2 scavenging activity and further nanoparticles proceeded with Bacterial Growth Inhibition NSNT/PP/25 and Minimum inhibitory concentration. The present work of biosynthesis of silver NANOPARTICLE SYNTHESIS AND nanoparticles using Alternanthera sessilis CHARACTERIZATION OF appears to be cost effective, eco-friendly, and ALTERNANTHERA SESSILIS an alternative to conventional method of K. SINDHU PRIYA synthesis. Research Scholar, Department of NSNT/PP/26 Biotechnology, School of life Science, Vels OPTIMIZATION AND University VISTAS, Pallavaram ChennaI. DECOLORISATION OF TEXTILE Mail id: [email protected] DYES USING THE EICCHORNIA SP. SYNTHESIZED SILVER/ZINC With expanding worldwide rivalries there is NANOCOMPOSITES a developing need to grow naturally big- hearted nanoparticles without utilization of ROSHINI R.G1 & M. GNANADESIGAN2 harmful synthetic conductions. The 1Department of Biochemistry, Bharathiar biosynthesis of silver nanoparticles (AgNPs) University, Coimbatore. 38 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) 2Department of Microbial Biotechnology, Zinc Sulphate: Silver nitrate (V:V:V) (ml)- Bharathiar University, Coimbatore. 2:4:4, Synthesizing Temperature 80 ˚C, Synthesizing Time 30 min, Molarity ration [email protected] (ZnSO4:AgNO3) 1M:1mM, Magnetic The present study was aimed to identify the Stirring rpm-300, Centrifugation rpm 4000, photocatalytic properties of the silver/zinc Drying temperature (˚C) 80. Further, nanocomposites using the Eicchornia sp. 100 structural analytical characteristics are also ppm of Coomassie brilliant blue dye was identified for the optimized composites. selected for the present study. The following From the present findings, the ZnO/Ag optimized conditions were selected for the Nanocomposite synthesized using the better decolorisatioan properties; Weight of Eicchornia crassipes leaf extract has showed the weed sample-10 g, Ratio of Weed extract: the affirmative (decolorisatioan percentage as 96%) results with the optimized parameters 39 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) BIOMEDICAL TECHNOLOGY (BMT) International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) BMT/PP/01 increases the sensitivity of docetaxel in TNBC. RUXOLITINIB CONJUGATED LIPOSOMES TO INCREASE THE BMT/PP/02 EFFICACY OF DOCETAXEL FOR THE TREATMENT OF TRIPLE NEGATIVE MOSQUITICIDAL ACTIVITY OF BREAST CANCER PANCHAGAVYA AGAINST ANOPHELES STEPHENSI, AEDES AGILANBALUPILLAI & ERNEST AEGYPTI AND CULEX DAVID QUINQUEFASCIATUS Department of Biotechnology, Thiruvalluvar SATHIYARAJ SIVAJIA, SURIYAKALA University, Serkadu, Vellore. GUNASEKARANA, DHANESH GANDHI [email protected] ARUMUGAMA, RANGANATHAN BABUJANARTHANAM Triple negative breast cancers (TNBCs) are RANGANATHANA* highly aggressive malignant form of epithelial breast tumours characterized by a&a* Nano and Energy Bioscience negative expression of estrogen receptor Laboratory, Department of Biotechnology, (ER), progesterone receptor (PR) and lack Thiruvalluvar University, Serkkadu, Vellore- gene amplification of HER2. Common 632115, Tamil Nadu, India. chemotherapeutic agent for the treatment of a TNBC includes docetaxel (DTX), paclitaxel * Corresponding Author: Dr R (PTX), cisplatin and doxorubicin etc. Babujanarthanam, However, there is a huge lacuna conventional Email: [email protected] chemotherapy due to dose dependent side effects and development of chemo-resistance Mosquitoes are one of the most important eventually limit the success of chemotherapy public health threats in the world. Several in TNBC. Despite recent advances, clinical mosquito species of the genera Anopheles, outcome of TNBC patients has not Aedes and Culex are carriers of pathogens of significantly improved. Therefore, various diseases, such as malaria, filariasis, combination therapies to target specific Japanese encephalitis, dengue and dengue oncogenic pathway has been needed to hemorrhagic fever, yellow fever and overcome drug resistance and to improve chikungunya. For several decades, synthetic clinical outcome. Persistent activation of insecticidal chemicals were used to kill these STAT3 is associated with cancer growth and vectors however, the repeated use of progression and also involved in cell synthetic insecticidal chemicals results in resistance. Therefore, Targeting JAK/STAT3 lower effectiveness and ecological. The signalling could be a potential novel effects of synthetic insecticidal chemicals therapeutic approach for treating advanced and the development of mosquito resistance and chemo resistant TNBC. Here, for the first have prompted the necessity of searching for time we planned to investigated the new insecticidal substances and alternative therapeutic potential of ruxolitinib, a methods, as novel biological tools. In order to JAK1/JAK2 inhibitor that conjugated produce new insecticidal substances, we use liposome increases the efficacy of docetaxel an effective product with insecticidal for the treatment of triple negative breast properties from the cow namely cancer. Thus, liposome mediated co-delivery “panchagavya” to control various genera of ruxolitinib/docetaxel significantly to mosquitoes. The aim of the present study to 40 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) evaluate the larvicidal and the pupicidal South India. The aim of present study was to activities of panchagavya. For the evaluation describe the epidemiology of rickettsial first- to fourth-instar larvae and pupae of diseases in two different areas of Vellore Anopheles stephensi, Aedes aegypti and district, Tamil Nadu, India. The study was Culex quinquefasciatus were used. The larval done to investigate the distribution of and pupal mortality was observed after 24 h ectoparasites among the predominant of exposure of panchagavya and the LC50 and reservoir hosts like rodents and domestic LC90 were calculated. Based on the results animal in the endemic area for Rickettsial panchagavya have more effective against infections and related infections. A total of Anopheles stephensi when compare to Aedes 156 ticks were collected from dogs (n = 6), aegypti and Culex quinquefasciatus. This cow (n = 12) and goats (n = 26). A subset of result suggests that the panchagavya have the 14 ticks was subjected to Nucleic acid potential to be used as an ideal eco-friendly extraction. PCR was performed to amplify approach for the control of vector 18SrDNA, the conserved region of the mosquitoes. arthropod. Among the 14 samples sequenced, only good quality sequences were chosen for Keywords: Panchagavya, Anopheles further analysis. BLAST analysis against the stephensi, larvicidal, vector control. reference sequence in the database identified BMT/PP/03 two different tick species Rhipicephalus zambesiensis from a cow, and A PILOT STUDY ON TICKS FROM Haemaphysalis hysteric from a goat. This is DOMESTIC ANIMALS IN TAMIL the first study to show the prevalence of NADU, SOUTH INDIA species associated with Rickettsial infection KALAIMANI SARAVANAN1& 2, RAMKI in South India. Further, morphological RAJENDRAPRASAD2, ANITHA 2, identification of species is essential to ERNEST DAVID1, RAJASEKAR1, JOHN correlate the sequence results. 2 ANTONY JUDE PRAKASH Keywords: Rickettsia Disease; spotted fever 1. Department of Biotechnology, BMT/PP/04 Thiruvalluvar University, Serkkadu, Vellore 632115, Tamilnadu. ANTIHYPERGLYCEMIC AND ANTIOXIDANT EFFECT OF ALLIUM 2.Department of Microbiology, Christian SATIVUM AQUEOUS BULB EXTRACT Medical College, Vellore 632004, Tamilnadu. AGAINST ALLOXAN-INDUCED DIABETIC MALE ALBINO RATS *Corresponding Author Ernest David, 1 Professor & Head, Dept of Biotechnology, S. SURESHKUMAR , Thiruvalluvar University, Vellore. B. SENTHILKUMAR2 Email: [email protected] 1Department of biochemistry Indo-American Spotted fever is a vector borne disease caused College, cheyyar-604407 by Rickettsia. This bacterium transmitted to 2 humans by arthropods mainly ticks. These Department of Zoology, Thiruvalluvar infections have re-emerged in India but the University, Serkkadu, Vellore-115. distribution of the tick vectors causing Herbal drugs play an important role in health rickettsial infection has not been studied in programs worldwide and there is a 41 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) resurgence of interest in herbal medicines for were collected from Madurai market. It was the treatment of various ailments including extracted by using methanol and water. diabetes mellitus. In both insulin-dependent Phytochemical analysis of extract showed the (Type 1) and non-insulin-dependent diabetes presence of betacyanin, alkaloids, steroids, (Type 2), an increased oxidative stress has terpenoids and tannins. The functional groups been notified. In the present study, the were analysed by using FTIR. FTIR analysis folklore medicinal plant Allium sativum was showed the presence of alkyl halides, selected to evaluate its antihyperglycemic alkynes, aliphatic amines, aromatics, alkenes and antioxidant effect against alloxan- and carboxylic acids. Of these squalene is one induced diabetic male albino rats. The of the major compounds which is responsible biochemical parameters such as blood the larvicidal activity. glucose, insulin, and lipid profile Tagetes extract possess antibacterial activity. (cholesterol, triglycerides, high-density The methanol extract of the flower (JFM) lipoprotein, low-density lipoprotein (LDL) was found to possess antimicrobial activity and very LDL, urea, uric acid, and creatinine) against a number of bacteria with inhibition were analyzed. Finally, the antioxidant zone diameters ranging from 9 to 20 mm, the parameters such as superoxide dismutase, bioassay-guided fractionation of which led to catalase, glutathione peroxide, and the isolation of a flavonoid patuletin in high glutathione S-transferase also analyzed. The yield as the active antibacterial principle with aqueous extract shows very good antidiabetic minimum inhibitory concentration (MIC) activity confirmed by biochemical value of 12.5 μ g/disk parameters and histopathological analysis. against Corynebacterium. Keywords: Allium sativum, Alloxan, spp., Staphylococcus. spp., Streptococcus. antioxidant, Diabetes spp., and Micrococcus luteus.. BMT/PP/05 Ethyl acetate extract was effective towards larvae of Aedes agepti. LC and LC values ASSESSMENT OF EFFICACY OF 50 90 were 6.441µg/ml & 23.216 µg/ml for 24 Tagetes PLANT EXTRACT AGAINST hours. For 48 hours LC and LC values MOSQUITO LARVAE AND 50 90 were 3.996 µg/ml & 12.885 µg/ml and for PATHOGENIC BACTERIA 72 hours LC50 and LC90 values were V. RAJESHWARI, decreased compared to that of 24 hours and 48 hours were 1.923 µg/ml and 4.729 µg/ml. T. SHANMUGAPRABHA*. In search of alternative and safe methods of Department of Microbiology,Virudhunagar controlling dengue vector mosquito products, Hindu Nadars’ Senthikumara Nadar College Tagetes might prove to be a good vector (A), Virudhunagar – 626001. control tool which might be more safe to use and cost effective. The present study suggests Email: [email protected] Tagetes ethyl acetate leaf extract act as good Tagetes plant is used as an insecticide. The mosquito repellent. leaves and flowers of this plant possess antibacterial, antifungal and anticancer activity. In our research leaves of Tagetes 42 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) Keywords: Tagetes plant, antibacterial, BMT/PP/07 antifungal and anticancer activity, good TRANSCIENT EXPRESSION OF mosquito repellent. BOTULINUM NEUROTOXIN BMT/PP/06 SEROTYPE A1 (BONT/A1) OF RECEPTOR BINDING DOMAIN (RBD) CLONING AND EXPRESSION OF VP28 PROTEIN IN PLANTA GENE IN SPINACH THROUGH AGROINFILTRATION TECHNIQUE P. THENMOZHI, M. SUSITHRA USING PSIM24-EGFP VECTOR PRIYADARSHNI, C. PRAKASH & M.C. HARISH* M. ASHA, E. SONIYAGANDHI, V. VIJAY, J. BABY SHALINI &M.C. HARISH* Plant Genetic Engineering Lab Plant Genetic Engineering Lab Department of Biotechnology, Thiruvalluvar University, Serkkadu. Department of Biotechnology, Thiruvalluvar University, Serkkadu. Botulism is a neuroparalytic disease and Botulinum neurotoxin is a causative agent of White spot syndrome (WSS) is a deadly Botulism and cause severe neuroparalytic disease caused in shrimp by WSS Virus disease by the active inhibition of affecting shrimp aquaculture with high neurotransmitter release in peripheral mortality rate. WSSV have five major cholinergic nervous system which leads to proteins such as VP26, VP24 & VP15 in paralysis. Botulinum neurotoxin is nucleocapsid and VP19 & VP28 in envelope. potentially more toxic to human and also These proteins play an important role in world threatening bioterrorist agent. binding to shrimp membrane, which leads to Botulinum neurotoxin consists of 150 kDa the WSSV infection. In the present study we polypeptide protein includes heavy chain have taken the WSSV VP28 protein as a (100 kDa) and light chain (50 kDa) joined by candidate for oral vaccine development in disulphude bond. There are seven BoNT planta to induce immunity in shrimp. To serotypes (A-G) and 40 sub serotypes. The achieve this we have cloned and expressed serotype botulinum neurotoxin A (BoNT/A) VP28 gene in spinach plant through belongs to the most dangerous class of agroinfitration using pSiM24-eGFP plant bioweapons. This toxin exposure to human expession vector and confirmed by the N- rapidly causes the flaccid paralysis and terminal His tag site and subsequently 28 6 autonomic dysfunctions. The HCC regions of kDa VP28 protein was confirmed by SDS BoNT/A consist of Receptor Binding Page. Further the stable spinach lines will be Domain which binds to Gangliosides (GT1b) used as a feed based vaccine against WSSV and with SV2 membrane in particular in shrimp. synaptic vesicle glycoprotein SV2C, which is Key words: White spot syndrome virus, responsible for above clinical conditions. The VP28, Transient expression objective of the present study is to develop “Plant made (expressed) receptor binding domain as a vaccine candidate which will acts as toxin like particle inducing antibody 43 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) synthesis in host and blocking of BoNTA1 novel HGT events among distinct lineages binding on SV2 membrane, hence not using next-generation sequencing. Most effecting neurotransmission process”. examples to date include gene transfers from Initially the BoNT/A1-RBD gene was bacterial donors to recipient organisms successfully cloned pSiM24-eGFP based including fungi, plants, and animals. In expression vector and transformed plants, one well-studied example of HGT is Agrobacterium. Later the RBD was the transfer of the tumor-inducing genes (T- successfully expressed DNAs) from some Agrobacterium species in Nicotiana benthamiana by transient into their host plant genomes. Evidence of T- expression through Agroinfiltration and RBD DNAs from Agrobacterium spp. into plant protein expression in planta was confirmed genomes, and their subsequent maintenance with SDS and western blotting using His tag in the germline, has been reported antibodies. Further the purified RBD protein in Nicotiana, Linaria and, more recently, in ready for animal studies. in Ipomoea species. The transferred genes do not produce the usual disease phenotype, and Keywords: Botulinum neurotoxin, Receptor appear to have a role in evolution of these binding domain, Gangliosides interaction, plants. In this paper, we review previous synaptic vesicle protein interaction. reported cases of HGT from Agrobacterium, BMT/PP/08 including the transfer of T-DNA regions from Agrobacterium spp. to the sweetpotato HORIZONTAL GENE TRANSFER [Ipomoea batatas (L.) Lam.] genome which CONTRIBUTES TO PLANT is, to date, the sole documented example of a EVOLUTION: THE CASE naturally-occurring incidence of HGT OF AGROBACTERIUM T-DNAS from Agrobacterium to a domesticated crop DORA G. QUISPE- plant. We also discuss the possible HUAMANQUISPE1,2, GODELIEVE evolutionary impact of T-DNA acquisition GHEYSEN1 & JAN F. KREUZE2* on plants. 1Department of Molecular Biotechnology, BMT/PP/09 Ghent University, Ghent, Belgium EXTRACTION AND PURIFICATION 2International Potato Center (CIP), Lima, OF CAROTENOID FROM BANANA Peru LEAVES FOR ITS ANTIOXIDANT AND MICROBIAL BIOAUTOGRAPHY Horizontal gene transfer (HGT) can be defined as the acquisition of genetic material ELAIYARAJA R1*, ANANDARAJ K1, from another organism without being its SATHIYASEELAN K1 & MANI A2* offspring. HGT is common in the microbial *Corresponding Authors world including archaea and bacteria, where HGT mechanisms are widely understood and Email: [email protected] recognized as an important force in 1Assistant Professor in Microbiology, evolution. In eukaryotes, HGT now appears to occur more frequently than originally 2 Ph.D Research Scholar in Microbiology, thought. Many studies are currently detecting 44 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) PG & Research Department of Microbiology, kamban college of arts and science for Shanmuga Industries Arts & Science college, women - Thiruvannamalai Tiruvannamalai, Tamil Nadu, India. Plant natural products are low molecular Banana (Musa acuminata) is one of the weight compounds playing important roles in largest produced fruit after citrus, hence plant survival under biotic and abiotic about 16% of the world’s total fruit stresses. In the rhizosphere, several groups of production. India is one of the major plant natural products function as producers of banana, supplying about 27% of semiochemicals that mediate the interactions world’s banana production. In Tamil Nadu is of plants with other plants, animals and the leading producer of banana, followed by microorganisms. The knowledge on the Maharashtra. Banana leave are also a good biosynthesis and transport of these signaling source of lignin, which is higher than banana molecules is increasing fast. This enables us pseudo stem. Waste materials from banana to consider to optimize plant performance by plant like a leaf blade, floral stalk, leaf changing the production of these signaling sheaths and others are unable to used for molecules or their exudation into the pulping due to relatively high amount of ash rhizosphere. Here we discuss recent advances content (18-28%). Good amount of in the understanding and metabolic pentosans are present in petiole, leaf blade, engineering of these rhizosphere and leaf sheaths. semiochemicals. Carotenoids have represented another possibility for the food and pharmaceutical industry. In practice of post-harvest residues is a good option for obtaining biosubstances of interest. The implementation of enzymatic pretreatment over plant matrices has resulted in an increase in the quantity of extraction and lowered operating costs. Banana waste contain good amount of exogenous antioxidants arises, as nutritional supplements or pharmaceutical products, which contain as active principle an antioxidant compound. In this study to extract and characterize the carotenoids from yellow and dried banana leaves waste and its assay of antioxidant and microbial bioautography. Key Words: Banana leaves, Carotinoids, petiole & Bioautography. BMT/PP/10 T. BHUVANESHWARI 45 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) MICROBIOLOGY & ENVIRONMENTAL MEB/PP/51 BIOTECHNOLOGY ANTIMICROBIAL PEPTIDES IN CONTROLLING BIOFILM MEB/PP/50 FORMATION BIOREMEDIATION OF J. SABIHA BANU ELECTROKINETICS & CHROMIUM Kamban College Of Arts And Science For S. LOKESHWARAN, R. Women, Thiruvannamalai. MADHANKUMAR, & ARULIAH RAJASEKAR1* Biofilm is a consortium of microbial cells, produced/protected by means of physical Adiparasakthi Collage Of Arts & Science appendages and extracellular polymeric Kalavai, Ranipet 632506 matrix, considered a global challenge due to Environmental Molecular Microbiology the inherent antibiotic resistance and Research Laboratory, Department Of environmental threats leading to cause huge Biotechnology, Thiruvalluvar University, damage in food industries, fisheries, Serkkadu, Vellore 632115, Tamilnadu, India. refineries, water systems, pharmaceutical industry, medical industry, etc conferred by *Corresponding Author: Email: its lifestyle. Living with a community of [email protected] microbial population is most critical in the clinical field, make it responsible for 80% of In this study, the electrokinetic (EK) severe, non-treatable, highly complicated and remediation, of tannery industrial Cr (VI) chronic microbial diseases. Thus far, many contaminated soil were collected from approaches have been attempted to tackle / Ammoor village, ranipetai, Tamilnadu, India. and to disrupt the initial stage of biofilm The experimental cell setup mated in 24×4×6 formation, including adherence and cm3 dimension are divided in three maturation. The embarrassment of antibiotic compartment one is soil compartment and resistance, extreme environmental and another two is electrolyte compartment. The chemical tolerance in clinical settings has middle compartment was loaded in tannery been further provoking by the ability of contaminated soil and distill water electrolyte pathogenic bacteria to produce biofilm were added anode and cathode formation. Necessity to find the alternative compartments. The bacteria sample were approach is the need of hour to solve these added catholyte compartment. DC power crises. supply was under a constant voltage gradient (20 V cm−1) noted every 5 mins mA. The Antimicrobial peptides are subset of human temperature difference in soil-cell positions defense peptides (HDP), evolutionary was likely caused by a variety of soil conserved in all life and acting as the first line characteristics, such as ionic strength. After of defense molecules secreted by various the Bio-Electrokinetic remediation defense cells against invading pathogens into experiment results was showed Chromium the host. AMPs demonstrate both direct (VI) – 80%, Chloride – 74% and sulphate – (antimicrobial and antibiofilm) or indirect 68% removal percentage at end of 4 days. (immunomodulatory/anti-inflammatory) 46 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) protection against pathogens including MEB/PP/53 bacteria, fungi, viruses, parasites either alone BIODEGRADATION ON VERMI or with other drugs than their antibiotic SOLUTION IN MANAGEMENT OF counterparts. For an example, the cathelicidin MUNICIPAL WASTE LL-37 was the first identified AMP with antibiofilm activity against Pseudomonas M.LOGANATHAN 1 (Department of aeruginosa Zoology Thiruvalluvar University Constituent arts and Science college, Key words: Extracellular polymeric matrix, Attupakkam, Arakkonam-631 051) antibiotic resistance, food industries, [email protected] antimicrobial peptide, Antibiofilm activity M.ARUNA 2 N. JOTHI NARENDIRAN 3 MEB/PP/52 (PG & Research Department of Zoology T. BHUVANESHWARI and Biotechnology,Government Arts College kamban college of Arts and science for For Men (Autonomous)Nandanam, Chennai. women Thiruvannamalai. Pollution caused by municipal solid waste The rhizosphere is arguably the most had become a serious problem and causes complex microbial habitat on earth, health hazards. Vermitechnology based comprising an integrated network of plant municipal waste management had become roots, soil and a diverse microbial consortium possible and gains attention. This chapter of bacteria, archaea, viruses, and focuses on the potential of earthworms microeukaryotes. Understanding, predicting Eudrilus eugeniae, Eisenia fetida and and controlling the structure and function of Perionyx excavatus in management of the rhizosphere will allow us to harness plant- municipal sludge collected from various parts microbe interactions and other rhizosphere of study area using vermitechnology. activities as a means to increase or restore Observed data suggest considerable decrease plant ecosystem productivity, improve plant in toxic metals compared with the initial responses to a wide range of environmental levels, the concentrations of heavy metals perturbations, and mitigate effects of climate including Zn, Cu, Pb, Mn, Cr, Ni and Co has change by designing ecosystems for long- decreased noticeably in E.eugeniae treated term soil carbon storage. Here, we review municipal sludge. Similar effect was also critical knowledge gaps in rhizosphere observed in P. excavatus, when compared to science, and how mechanistic understanding E. eugeniae and E .fetida the latter was found of rhizosphere interactions can be leveraged to be effective in reducing the metal toxicity in rhizosphere engineering efforts with the of the sludge; also, the earthworm enriches goal of maintaining sustainable plant the sludge with various nutrients essential for ecosystem services for food and bioenergy plant and microbial growth. The soil and production in an ever changing global heavy metal dynamics shows a significant climate. decrease, which draws a conclusion to exploit vermitechnology in bio management of municipal sludge using selected earthworm species. 47 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) MEB/PP/54 BIOREMEDIATION R. ANJU, P. BHUVANANAYAGI Marudhar Kesari Jain College For Women, Vaniyambadi. Bioremediation is the use of either naturally occurring or deliberately introduced microorganisms to consume and break down environmental pollutants, in order to clean a polluted site. It is used to treat contaminated media, including water, soil and subsurface material, by altering environmental conditions to stimulate growth of microorganisms and degrade the target pollutants. Bioremediation is less expensive and more sustainable than other remediation alternatives. Biological treatment is a similar approach used to treat wastes including wastewater, industrial waste and solid waste. Bioremediation has been highly successful in order to clean up pollutants including crude oil, gasoline, pesticides, sewage, and chlorinated solvents used in cleaning supplies. 48 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) Author Index International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) AUTHOR NAME PAGE NUMBER A AADIL AHMED I 20 ABBAS ALAM CHOUDHURY 18 AGILANBALUPILLA 39 AKILA S 11 AMEEMA F 11 ANITHA 40 ANANDARAJ K 43 ANANTHASELVAM AZHAGESAN 4 ANITHA. J 6 ANJALI S 16 ANJU R 48 ANURADHA K 36 ARULJOTHI A 10 ARUL PRAKASH ARUMUGAM 2 ARUNA M 47 ARUNA DEVI S 15 ASHA M 42 AYESHA SIDDIQUA 35 B BABUJANARTHANAM RANGANATHAN 8,30,32,39 BABY SHALINI J 42 BALASUBRAMANIAN A 5 BALUSAMY. G 4 BHARATHI S 36 BHUVANANAYAGI P 48 BHUVANESHWARI P 33 BHUVANESHWARI T 45 C CHELLAPANDI T 32 CHAMBA MARGARET M 19 CHANDRASEKARAN NATRAJAN 4 CHANDRU S 11 CHARUMATHI D 7 CHETAN L 8 D DEIVANAYAKI M 15 DEVI PRIYA D 33 DEIVASIGAMANI BALARAMAN 35 DHANDAPANI P 5,25 DHANDAPANI R 13 DHANESH GANDHI ARUMUGAM 30,39 E ELAIYARAJA R 43 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) ERNEST DAVID 27, 28, 34,39,40 F FAROOK M A 20 FOWSIYA J 32 G GAYATHRI G R 21 GAYATHRI DEVI K 7 GNANADESIGAN M 37 GODELIEVE GHEYSEN 43 GOKUL S 11 GOKULA PRIYA N 35 GOWTHAM D M 9 H HARIKRISHNAN S 19 HARISH M C 42 HEMAPRIYA J 18 I INBASEKARAN V 14 INDHUJA R 16 J JABEZ OSBORNE W 23 JAN 43 JANANI M 5 JANANI T 17 JAYABHARATHI THIRUMALAZHAGAN 35 JAYACHANDRAN K 15 JAYANTHI V 30 JEYA KUMAR S 17 JAYALAKSHMI S 19 JAYAPRAKASH K 10,11 JENNIFER JEBA KIRUBA C 17 JOHN ANTONY JUDE PRAKASH 40 JOTHI NARENDIRAN N 47 K KALAIMANISARAVANAN 35 KAMALI SHREE DHARANI E 12 KANIMOZHI S 33 KARUNA K 22 KAVITHA C 21 KAVIYARASAN V 12 KOKILARAMANI SEENIVASAN 3 KOUSHIKA.S 9 KREUZE F 43 KRISHNA KUMARI G N 12 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) KUMAR J 22 L LAVANYA S 21 LAVANYA THOPIREDDY 29 LOGANATHAN M 47 LOKESHWARAN S 46 M MADHANKUMAR R 46 MADHUMITHA G 31,32 MADHUMITHA S 17 MAHALAKSHMI K 34 MANI A 43 MANI G 26 MANI U 35 MANIKANDAN E 26 MANIKANDAN S 14 MANJUNATHAN J 12 RAJASEKAR B 5 MOHAMMED HABEEB AHMED 29 MOHAMMED JAMALUDEEN A 11 MOHANA ROOPAN S 34 MONIKA 12 MUBASIRA BANU M J 12 MUNUSAMY RENUGOPAL 19 N NISHANTHRAJI R 10 NISHITHA A 7 P PARTHIPAN PUNNIYAKOTTI 1 POORNIMA A 12 PRAKASH C 42 PREETHI K 7,9 PRIYAYANGA B 19 PRIYANKA N 37 R RAHILA E 7 RAJALAKSHMI A M 13 RAJARAM K 31 RAJASEKAR ARULIAH 1,2,3,4,5,19,21,25,40,46 RAJESHWARI V 41 RAJESWARA REDDY SADDALA 29 RAMACHANDRAN VINAYAGAM 27 RAMANI S 12 RAMESH M 26 RAMKI RAJENDRAPRASAD 40 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) RAMYA R 23 RAMYA GUNASEKARAN 18 RENUGA S 16 RITHIKA S 12 ROGINI M 36 ROSHINI R G 38 RUMANA S 12 S SABIHA BANU J 46 SACHIN J 21 SAKALA PATRICIA 35 SAKTHISWARI ASHOKAN 8 SAKTHIVEL KUMAR 19 SANGEETHA SUBRAMANIAN 6,29,30 SANDHIYA L 24 SANDHIYA K 36 SANIYA Y 14 SANTHIYA S SANTHIYA SIGA S 24 SANTHOSHKUMAR MURALI 28,34 SARANKUMAR RAJA KUMARESAN 1 SARGUNAN V 14 SATHISH VENKATESAN 35 SATHIYARAJ SIVAJI 8,30,32,39 SATHIYASEELAN 43 SELVARAJ MOHANA ROOPAN 8 SELVI ADIKESAVAN 4,5 SELVAM K 26 SENTHIL S 22 SENTHILKUMAR B 41 SHANMUGAVEL M 35 SHANMUGAPRABHA T 41 SHANTHAPRIYA R 7 SHEELA K 26 SHYMALA GOWRI S 12 SINDHU S 17 SINDHU PRIYA K 37 SIVARAMAKRISHNAN S 22 SONIYAGANDHI E 42 SRINIVASAN R 26 SUBRAMANIA ANGAIAH 1 SUMITHRA S 36 SUGANYA R 21 SURESH G 14 SURESHKUMAR S 41 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) SURESH YARRAPPAGAARI 29 SURIYAKALA GUNASEKRAN 8,30,32,39 SURYA R 23 SUSITHRA PRIYADARSHNI M 42 SWARNKUMAR B 23 SWETHA A 24 SWETHA V 16 T TANUSREEDR N 7 TANZEELA N 14 THAMARAISELVI S 21 THANGAMANI V 36 THENMOZHI P 42 U UMASHANKAR G 21 URMILA GOPAL PARANTHAMAN 32 UVARAJAN S 26 V VAISHNAVI JEEVANANDAM 2 VASANTHAKUMAR K 25 VENKATACHALAM P 37 VELMURUGAN C 11 VIJAY V 42 VIJAYALAKSHMI S 27 VIJAYALAKSHMI V 14 VIJAYA LALITHA R 17 VIJAYANAND S 18 VIMALA R T V 22 VINOTH KUMAR PALUR MANOHARAN 5 Z ZAKIRA BANU S 34 International Seminar on “Recent Advances in Bioremedial Technology’’(RABT-2020) ADVERTISEMENT WITH BEST COMPLIMENTS FROM THE PRECISION SCIENTIFIC CO,(CBE) AUTHORISED DEALERS & STOCKISTS FOR CHEMICALS : RANBAXY,NICE,HIMEDIA,LOBA,MERCK,SRL,GENEI, SIGMA,ACROS. GLASSWARES : BOROSIL & DURAN INSTRUMENTS :OLYMPUS,NSW,REMI,SYSTRONICS,ELICO,SHIMADZU & IMPORTED INSTRUMENTS. THE ONE STOP SHOP FOR ALL YOUR LABORATORY NEEDS 503,VEERA BOYAR COLONY, BRANCH: DR.NANJAPPA ROAD, B.O:9L/7,VINAYAGA COIMBATORE-641 018. COMPLEX, Ph:2235970, 2237280,4506280 MADURAI ROAD, E.Mail:[email protected] TILAK NAGAR, [email protected] TIRUNELVELI-627 001. Ph.N.o.0462-2321732 BRANCH : [email protected] C-6,2ND CROSS, THILLAI NAGAR, BRANCH: TRICHY-620 018. 48,SIVAM NAGAR, Ph.No:2764014,2750831. THIRUVARUR-610 001. [email protected] Ph.No.04366-221014 [email protected]