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Determination of some hematologic effect the Persian Gulf blackfin stonefish, (Pseudosynanceia melanostigma), venom on albino mice blood Item Type thesis Authors Zolfaghari, Mohammad Publisher Islamic Azad University, Science and Research Branch, Tehran, Marine Biology Download date 05/10/2021 00:41:10 Link to Item http://hdl.handle.net/1834/36477 معاونت پژوهش و فن آوري به نام خدا منشور اخﻻق پژوهش با ياري از خداوند سبحان و اعتقاد به اين كه عالم محضر خداست و همواره ناظر بر اعمال انسان و به منظور پاس داشت مقام بلند دانش و پژوهش و نظر به اهميت جايگاه دانشگاه در اعتﻻي فرهنگ و تمدن بشري، ما دانشجويان و اعضاء هيات علمي واحدهاي دانشگاه آزاد اسﻻمي متعهد ميگرديم اصول زير را در انجام فعاليت هاي پژوهشي مد نظر قرار داده و از آن تخطي نكنيم: 1- اصل حقيقت جويي: تﻻش در راستاي پي جويي حقيقت و وفاداري به آن و دوري از هرگونه پنهان سازي حقيقت. 2- اصل رعايت حقوق: التزام به رعايت كامل حقوق پژوهشگران و پژوهيدگان )انسان، حيوان و نبات( و ساير صاحبان حق 3- اصل مالكيت مادي و معنوي: تعهد به رعايت كامل حقوق مادي و معنوي دانشگاه و كليه همكاران پژوهش 4- اصل منافع ملي: تعهد به رعايت مصالح ملي و در نظر داشتن پيشبرد و توسعه كشور در كليه مراحل پژوهش 5- اصل رعايت انصاف و امانت: تعهد به اجتناب از هرگونه جانب داري غير علمي و حفاظت از اموال، تجهيزات و منابع در اختيار 6- اصل رازداري: تعهد به صيانت از اسرار و اطﻻعات محرمانه افراد، سازمانها و كشور و كليه افراد و نهادهاي مرتبط با تحقيق 7- اصل احترام: تعهد به رعايت حريمها و حرمتها در انجام تحقيقات و رعايت جانب نقد و خودداري از هرگونه حرمت شكني 8- اصل ترويج : تعهد به رواج دانش و اشاعه نتايج تحقيقات و انتقال آن به همكاران علمي و دانشجويان به غير از مواردي كه منع قانوني دارد. 9- اصل برائت: التزام به برائت جويي از هرگونه رفتار غيرحرفهاي و اعﻻم موضع نسبت به كساني كه حوزه علم و پژوهش را به شائبههاي غيرعلمي ميآﻻيند. أ دانشگاه آزاد اسﻻمي واحد علوم و تحقيقات تعهد نامه اصالت رساله يا پايان نامه اينجانب محمد ذوالفقاري دانش آموخته مقطع كارشناسي ارشد ناپيوسته در رشته زيست شناسي دريا كه LD50 " در تاريخ 42/11/1931 از پايان نامه خود تحت عنوان استخراج و تعيين زهر ماهي فرياله خال سياه خليج فارس و بررسي عﻻئم هماتولوژيكي ايجاد شده توسط زهر در موش آزمايشگاهي" با كسب نمره 1/11 و درجه ....................... دفاع نموده ام بدينوسيله متعهدمي شوم: 1( اين پايان نامه / رساله حاصل تحقيق وپژوهش انجام شده توسط اينجانب بوده ودر موواردي كوه از دستاوردهاي علمي وپژوهشي ديگران )اعم از پايان ناموه، كتواب، مقالوه و......( اسوتفاده نمووده ام، مطابق ضوابط ورويه موجود ، نام منبع مورد استفاده وساير مشخصات آن را در فهرست مربوط ذكر و درج كرده ام. 4( اين پايان نامه / رساله قبﻻ براي دريافت هيچ مدرک تحصيلي )هم سطح، پايين تر يا باﻻتر( درساير دانشگاه ها وموسسات آموزش عالي ارائه نشده است. 9( چنانچه بعد از فراغت تحصيل قصد استفاده وهرگونه بهره برداري اعم از چاپ كتاب ، ثبت اختراع و ....ازاين پايان نامه داشته باشم، از حوزه معاونت پژوهشي واحد مجوزهاي مربوطه را اخذ نمايم. 2( چنانچه در هر مقطع زماني برخﻻف موارد فوق ثابت شود ، عواقوب ناشوي ازآن را موي پوذيريم و واحد دانشگاهي مجاز است با اينجانب مطابق ضوابط ومقررات رفتوار نمووده ودر صوورت ابطوال مدرک تحصيلي ام هيچگونه ادعايي نخواهم داشت. نام ونام خانوادگي : محمد ذوالفقاري تاريخ و امضاء: ب دانشگاه آزاد اسﻻمي واحد علوم و تحقيقات )تهران( دانشکده علوم پایه-گروه زیست شناسي پایان نامه برای دریافت درجه کارشناسي ارشد گرایش جانوران دریا عنوان: استخراج و تعيين LD50 زهر ماهي فریاله خال سياه خليج فارس و بررسي عﻻئم هماتولوژیکي ایجاد شده توسط زهر در موش آزمایشگاهي اساتيد راهنما دکتر شهﻻ جميلي دکتر حسين ذوالفقاریان نگارش محمد ذوالفقاری پائيز 59 ت تقدير و تشكر بود اول آن خجسته پرگار نام ملكي كه نيستش يار سپاس و ستايش تو راست اي خالق بي همتا كه همواره الطاف بي كرانت روشنگر راهم بوده و نيروي ﻻيزال تو گشاينده مشكﻻت. امروز هر چه دارم از مهربانيها و بخشندگيهاي توست. افسوس كه زبان قاصر از حمد توست، زيرا كه تو بي نهايتي و من ذرهاي از كران بي كران تو. با تشكر از حمايت هاي اساتيد راهنماي ارجمند جناب آقاي دكتر حسين ذوالفقاريان و سركارخانم دكتر شهﻻ جميلي به خاطر راهنمائي ها و پشتيباني هميشگي شان در طي اين تحقيق و كمک هاي ايشان در جهت پيشرفت توانائيهايم. و با سپاس ويژه از آقايان مهندس هدايت، شهبازي، ربيعي و خيراله پور كه در جهت پيشرفت اين تحقيق از هيچ كمک و راهنمائي دريغ نكرده و صبورانه تجارب علمي ارزشمندشان را در اختيارم گذاشتند. از اعضاي محترم هيئت علمي گروه زيست شناسي دانشگاه آزاد اسﻻمي واحد علوم و تحقيقات و نيز هيئت علمي مؤسسه تحقيقات واكسن و سرم سازي رازي كرج، رياست و كارمندان محترم بخش سروتراپي و جانوران سمي بويژه مهندس ربيعي و هدايت سپاسگزارم. موفقيت امروز من بدون حمايت هاي اساتيد بزرگوار در طي ساليان تحصيلم امكان پذير نبود، از ايشان و از تمامي دوستانم به خاطر تمام اوقاتي كه با هم داشتيم، صميمانه تشكر مي كنم. ث تقديم به دستان مهربان مرحومه مادر عزيزم كه موفقيت و ارتقاي علمي فرزندانش منتهاي آرزويش بود، به پاس آسايشي كه در جهت آسايش ايشان از خود دريغ كرد. تقديم به روح پدر مرحوم و عزيزم كه استواري را از او آموختم. راهنمايي او همواره روشن گر مسير زندگيم بود و موفقيت امروز من بدون حمايت هايش ممكن نبود. تقديم به روح پرفتوح همسر مهربانم كه در نقاط محروم و دور دست هميشه يار و ياورم بود. ج فهرست مطالب چكيده .................................................................................................................................................. 1 فصل اول: كليات ................................................................................................................................ 4 1- مقدمه ............................................................................................................................................. 9 1-1 ماهيان زهري ................................................................................................................................ 6 1-4 ماهيان زهري با سموم برون ريز ................................................................................................. 6 1-9 ماهيان زهري .............................................................................................................................. 1 1-2 خليج فارس و استان بوشهر ......................................................................................................... 8 1-1 آبزيان خليج فارس ...................................................................................................................... 14 1-6 نحوه ي عملكرد سموم ................................................................................................................ 14 1-1 گروه سنگ ماهيان )فرياله ها( ...................................................................................................... 16 1-8 جايگاه فرياله خال سياه خليج فارس در سلسله جانوري و پراكنش جهاني آن .......................... 13 1-3 مورفولوژي فرياله ي خال سياه و مسموميت با زهر آن ............................................................... 42 1-12 اهداف پژوهش ........................................................................................................................ 44 1-11 اهدافي كه پس از اتمام پژوهش دنبال خواهند شد ................................................................... 44 فصل دوم: مروري بر ادبيات و پيشينه تحقيق ................................................................................... 49 فصل سوم: مواد و روشها ................................................................................................................ 99 9-1 روش هاي آزمايشگاهي ............................................................................................................... 92 9-1-1 تخليص ................................................................................................................................... 92 9-1-4 دياليز ....................................................................................................................................... 91 9-1-9 سانتريفوژ ................................................................................................................................ 96 9-1-2 اسپكتروفتومتري ...................................................................................................................... 98 ح 9-1-1 پروتئين سنجي ...................................................................................................................... 22 9-1-6 الكتروفورز ............................................................................................................................ 21 9-1-1 مواد تسهيل كننده حﻻليت پروتئينها در الكتروفورز ............................................................ 22 9-1-8 سديم دودسيل سولفات پلي اكريل آميد ژل الكتروفورز )SDS-PAGE( ............................. 22 9-1-3 ساختمان ژل پلي اكريل آميد ................................................................................................ 26 9-1-12 انواع ژل هاي استفاده شده در الكتروفورز .......................................................................... 28 9-1-11 تكنيک هاي رنگ آميزي ژل ............................................................................................... 23 9-1-14 روش سنجش لخته ............................................................................................................ 14 9-1-19 آزمايش هاي سيستم انعقادي ............................................................................................. 14 PT 9-1-19-1 زمان پروترومبين ) ( .................................................................................................. 14 9-1-19-4 زمان نسبي ترومبوپﻻستين فعال شده )aPTT( .............................................................. 19 9-1-12 هماتوكريت ........................................................................................................................ 19 9-1-11 سلول هاي قرمز خون ........................................................................................................ 12 9-1-16 سلول هاي سفيد خون ....................................................................................................... 12 9-1-11 هموگلوبين ........................................................................................................................
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    Original Article Injuries and envenomation by exotic pets in Hong Kong Vember CH Ng, Albert CH Lit, OF Wong *, ML Tse, HT Fung ABSTRACT effects, and six cases with mild effects. All major effects were related to venomous snakebites. There Exotic pets are increasingly popular Introduction: were no mortalities. in Hong Kong and include fish, amphibians, reptiles, and arthropods. Some of these exotic Conclusion: All human injuries from exotic pets animals are venomous and may cause injuries to arose from reptiles, scorpions, and fish. All cases of and envenomation of their owners. The clinical major envenomation were inflicted by snakes. experience of emergency physicians in the management of injuries and envenomation by these exotic animals is limited. We reviewed the clinical Hong Kong Med J 2018;24:48–55 features and outcomes of injuries and envenomation DOI: 10.12809/hkmj176984 by exotic pets recorded by the Hong Kong Poison 1 VCH Ng, FHKCEM, FHKAM (Emergency Medicine) Information Centre. 2 ACH Lit, FRCSEd, FHKAM (Emergency Medicine) Methods: We retrospectively retrieved and reviewed 2 OF Wong *, FHKAM (Anaesthesiology), FHKAM (Emergency Medicine) 1 cases of injuries and envenomation by exotic pets ML Tse, FHKCEM, FHKAM (Emergency Medicine) 3 HT Fung, recorded by the Hong Kong Poison Information FRCSEd, FHKAM (Emergency Medicine) Centre from 1 July 2008 to 31 March 2017. 1 Hong Kong Poison Information Centre, United Christian Hospital, Kwun Results: There were 15 reported cases of injuries Tong, Hong Kong 2 Accident and Emergency Department, North Lantau Hospital, Tung and envenomation by exotic pets during the study Chung, Lantau, Hong Kong period, including snakebite (n=6), fish sting (n=4), 3 Accident and Emergency Department, Tuen Mun Hospital, Tuen Mun, This article was scorpion sting (n=2), lizard bite (n=2), and turtle Hong Kong published on 5 Jan bite (n=1).
  • Database of Bibliography of Living/Fossil

    Database of Bibliography of Living/Fossil

    www.shark-references.com Version 16.01.2018 Bibliography database of living/fossil sharks, rays and chimaeras (Chondrichthyes: Elasmobranchii, Holocephali) Papers of the year 2017 published by Jürgen Pollerspöck, Benediktinerring 34, 94569 Stephansposching, Germany and Nicolas Straube, Munich, Germany ISSN: 2195-6499 DOI: 10.13140/RG.2.2.32409.72801 copyright by the authors 1 please inform us about missing papers: [email protected] www.shark-references.com Version 16.01.2018 Abstract: This paper contains a collection of 817 citations (no conference abstracts) on topics related to extant and extinct Chondrichthyes (sharks, rays, and chimaeras) as well as a list of Chondrichthyan species and hosted parasites newly described in 2017. The list is the result of regular queries in numerous journals, books and online publications. It provides a complete list of publication citations as well as a database report containing rearranged subsets of the list sorted by the keyword statistics, extant and extinct genera and species descriptions from the years 2000 to 2017, list of descriptions of extinct and extant species from 2017, parasitology, reproduction, distribution, diet, conservation, and taxonomy. The paper is intended to be consulted for information. In addition, we provide data information on the geographic and depth distribution of newly described species, i.e. the type specimens from the years 1990 to 2017 in a hot spot analysis. New in this year's POTY is the subheader "biodiversity" comprising a complete list of all valid chimaeriform, selachian and batoid species, as well as a list of the top 20 most researched chondrichthyan species. Please note that the content of this paper has been compiled to the best of our abilities based on current knowledge and practice, however, possible errors cannot entirely be excluded.
  • Universidade Federal Do Amazonas Instituto De Ciências Biológicas Programa Multi-Institucional De Pós-Graduação Em Biotecnologia

    Universidade Federal Do Amazonas Instituto De Ciências Biológicas Programa Multi-Institucional De Pós-Graduação Em Biotecnologia

    UNIVERSIDADE FEDERAL DO AMAZONAS INSTITUTO DE CIÊNCIAS BIOLÓGICAS PROGRAMA MULTI-INSTITUCIONAL DE PÓS-GRADUAÇÃO EM BIOTECNOLOGIA JULIANA LUIZA VARJÃO LAMEIRAS PRODUÇÃO DE SORO HIPERIMUNE PARA Potamotrygon motoro Müller & Henle, 1841 (CHONDRICHTHYES – POTAMOTRYGONINAE): VERIFICAÇÃO DA REAÇÃO-CRUZADA FRENTE ÀS PEÇONHAS DE OUTRAS ESPÉCIES DE ARRAIAS E DA NEUTRALIZAÇÃO DAS ATIVIDADES EDEMATOGÊNICA E MIOTÓXICA Manaus 2018 JULIANA LUIZA VARJÃO LAMEIRAS PRODUÇÃO DE SORO HIPERIMUNE PARA Potamotrygon motoro Müller & Henle, 1841 (CHONDRICHTHYES – POTAMOTRYGONINAE): VERIFICAÇÃO DA REAÇÃO-CRUZADA FRENTE ÀS PEÇONHAS DE OUTRAS ESPÉCIES DE ARRAIAS E DA NEUTRALIZAÇÃO DAS ATIVIDADES EDEMATOGÊNICA E MIOTÓXICA Tese apresentada à Universidade Federal do Amazonas como requisito para obtenção do título de Doutora pelo Programa Multi- institucional de Pós-graduação em Biotecnologia. Área de concentração: Biotecnologia para Saúde. Orientadora: Professora Dra. Maria Cristina dos Santos – UFAM Coorientador: Professor Dr. Oscar Tadeu Ferreira da Costa – UFAM Manaus 2018 ' Poder Executivo Ministerio da Educa�ao Universidade Federal do Amazonas Programa Multi-lnstitucionalde P6s-Graduacao em Biotecnologia 226a. ATADEDEFESADETESE No dia 27 de mar90 (ter9a-feira) de 2018, as 9hs, 11a sala de aula do Bloco ''G'', Setor Sul - UFAM. Juliana Luiza Varjao Lameiras defendeu sua Tese de Doutorado intitulada ''Produ�ao de Soro Hiperimune para Potamotrygon motoro Miiller & Henle, 1841 (Chondrichthyes - potamotrygoninae): verifica�ao da rea'rao-cruzada frente as pe'ronhas de outras especies de arraias e da neutraliza�ao das atividades edematogenica e miotoxica. '' Banca de Examinadores: Membros Parecer AIssina tura I I / ! --. Aprovada (f) Assinatura: ;· c:?M· <.... � � (__ _C, ..._,·, .\ f Prof a. Dra. Maria Cristina dos Santos - (Presidente) Reprovada ( ) CPF: 1.0'61J.f4 ( . t 7 C f ,_ o 2.
  • Envenimations Par Poissons Tropicaux En Nouvelle-Calédonie

    Envenimations Par Poissons Tropicaux En Nouvelle-Calédonie

    UNIVERSITE DE NANTES FACULTE DE MEDECINE Année 2012 N° 062 THESE pour le DIPLOME D’ETAT DE DOCTEUR EN MEDECINE Qualification en MEDECINE GENERALE par Amandine COQUET HOUDAYER née le 07 janvier 1984 à Saint Martin Boulogne Présentée et soutenue publiquement le 17 septembre 2012 Envenimations par poissons tropicaux en Nouvelle-Calédonie Etude rétrospective de cas sur 18 mois (janvier 2009 à juillet 2010) Evaluation des pratiques au Service d’Accueil des Urgences du Centre Hospitalier Territorial de Nouvelle-Calédonie JURY : Président : Monsieur le Professeur François RAFFI Directeur de thèse : Monsieur le Docteur Claude MAILLAUD Monsieur le Professeur Michel MARJOLET Monsieur le Professeur Gilles POTEL Monsieur le Docteur Thierry PETELET 1 RESUME: Les envenimations par poissons tropicaux représentent un motif fréquent de consultation en Nouvelle- Calédonie. Les raies armées, poissons-pierres, rascasses et ptérois sont principalement responsables des accidents. Nous avons colligé rétrospectivement les dossiers des passages aux urgences du Centre Hospitalier Territorial de Nouvelle-Calédonie de janvier 2009 à juillet 2010 pour ce motif, soit 51 piqûres de raie, 9 de poisson-pierre, 14 de rascasse et aucune de ptérois, afin de déterminer les caractéristiques cliniques de ces accidents. Nos résultats apparaissent comparables à ceux de la littérature. Un protocole codifie la prise en charge de ces envenimations aux urgences du CHT de Nouméa. Tous critères confondus, l’adhésion du personnel soignant du service à ce protocole apparait être de 44,2% aux mesures de première intention préconisées de manière systématique, et de 33,5% aux mesures à adapter au cas par cas. Néanmoins, les complications de ce type d’accident ne semblent pas plus fréquentes dans notre série que dans celles de la littérature.
  • Alphabetical Index

    Alphabetical Index

    Alphabetical Index After-Hours Telehealth Triage Guidelines | Pediatric | 2021 9 Bottle-fed Weaning Questions Contraception - Birth Control Pills 911 Symptoms Bottle-feeding Questions Contraception - Birth Control Shot (Depo) A Breast Symptoms (Female) - After Puberty Contraception - Emergency Breast Symptoms (Female) - Before Contraception - Implant Abdominal Injury Puberty Contraception - IUD Abdominal Pain - Female Breast Symptoms (Male) Cough Abdominal Pain - Male Breastfeeding - Baby Questions COVID-19 - Diagnosed or Suspected Acne Breastfeeding - Mother's Breast Symptoms COVID-19 - Exposure Aggressive and Destructive Behavior or Illness Breastfeeding - Mother's Medicines and Cracked or Dry Skin Alcohol Use or Problems Diet Cradle Cap Allergic Reactions - Guideline Selection Breastfeeding - Weaning Questions Croup Altitude Sickness Breastfeeding (CANADA version) - Baby Croup on Steroid Follow-up Call Anaphylaxis Questions Crying - 3 Months and Older Animal Bite Breath-Holding Spell Crying - Before 3 Months Old Animal or Human Bite Infection on Breathing Difficulty (Respiratory Distress) Antibiotic Follow-up Call Breathing Noisy - Guideline Selection Cuts and Lacerations Ankle Injury Bronchiolitis Follow-up Call D Anxiety and Panic Attack Bruises Diabetes - High Blood Sugar Arm Injury Burns Diabetes - Low Blood Sugar Arm Joint Swelling C Diaper Rash Arm Pain Carbon Monoxide Exposure Diarrhea Asthma Attack Cast Symptoms and Questions Diarrhea Diseases from Travel Asthma Attack on Steroid Follow-up Call Cellulitis on Antibiotic Follow-up