bioRxiv preprint doi: https://doi.org/10.1101/2020.02.03.933291; this version posted February 4, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Genome-resolved metagenomics and detailed geochemical speciation analyses yield 2 new insights into microbial mercury cycling in geothermal springs 3 4 RUNNING TITLE: Microbial mercury cycling in acid warm springs 5 6 Caitlin M. Gionfriddo1,+*, Matthew B. Stott2, #, Jean F. Power2, Jacob M. Ogorek3, David 7 P. Krabbenhoft3, Ryan Wick4, Kathryn Holt4, Lin-Xing Chen5, Brian C. Thomas5, Jillian 8 F. Banfield1, 5, 6 and John W. Moreau1, ‡ 9 10 1School of Earth Sciences, The University of Melbourne, Parkville, VIC, Australia 11 2GNS Science, Wairakei Research Centre, Taupō, New Zealand 12 3Wisconsin Water Science Center, U.S. Geological Survey, Middleton, WI, USA 13 4Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and 14 Biotechnology Institute, University of Melbourne, Victoria 3010, Australia 15 5Department of Earth and Planetary Science, UC Berkeley, Berkeley, CA, USA 16 6Department of Environmental Science, Policy, and Management, University of California, 17 Berkeley, California 94720, United States 18 19 20 21 22 23 Current affiliations: 24 +Oak Ridge National Laboratory, Biosciences Division, Oak Ridge, TN, USA 25 # School of Biological Sciences, University of Canterbury, Christchurch, New Zealand 26 ‡ School of Geographical and Earth Sciences, University of Glasgow, UK 27 28 *Corresponding author:
[email protected] 29 1 bioRxiv preprint doi: https://doi.org/10.1101/2020.02.03.933291; this version posted February 4, 2020.