Lab 19 – A560 – Fall 2015

I. Introduction Pituitary Gland II. Learning Objectives III. Slides and Micrographs A. General structure B. Parts 1. a. Divisions i. Pars distalis ii. iii. b. Cells i. Chromophobes ii. Chromophils 1) Acidophils 2) Basophils 2. a. Divisions i. Infundibulum stalk ii. Pars nervosa b. Cells i. IV. Summary Fig 20‐2, Junqueira, 13th ed. Lab 19 –Pituitary Gland A560 – Fall 2015 Slide 128: Pituitary, H&E I. Introduction II. Learning Objectives III. Slides and Micrographs A. General structure B. Parts 1. Anterior Pituitary anterior a. Divisions i. Pars distalis pituitary ii. Pars intermedia iii. Pars tuberalis posterior b. Cells pituitary i. Chromophobes ii. Chromophils 1) Acidophils 2) Basophils 2. Posterior Pituitary a. Divisions i. Infundibulum stalk pars intermedia ii. Pars nervosa b. Cells i. Pituicytes IV. Summary Lab 19 –Pituitary Gland A560 – Fall 2015 Slide 128 (Hi‐Res): Pituitary, H&E

I. Introduction II. Learning Objectives III. Slides and Micrographs A. General structure B. Parts 1. Anterior Pituitary a. Divisions i. Pars distalis ii. Pars intermedia iii. Pars tuberalis b. Cells i. Chromophobes anterior ii. Chromophils pituitary 1) Acidophils 2) Basophils 2. Posterior Pituitary posterior a. Divisions pituitary i. Infundibulum stalk ii. Pars nervosa b. Cells i. Pituicytes IV. Summary pars intermedia w/ remnants of Rathke’s pouch Lab 19 –Pituitary Gland A560 – Fall 2015 Slide 103: Anterior Pituitary, H&E I. Introduction II. Learning Objectives III. Slides and Micrographs A. General structure B. Parts 1. Anterior Pituitary a. Divisions i. Pars distalis ii. Pars intermedia iii. Pars tuberalis b. Cells posterior i. Chromophobes pituitary ii. Chromophils 1) Acidophils pars 2) Basophils intermedia 2. Posterior Pituitary a. Divisions i. Infundibulum stalk anterior pituitary ii. Pars nervosa b. Cells i. Pituicytes IV. Summary Lab 19 –Pituitary Gland A560 – Fall 2015 Slide 122: Pituitary, H&E I. Introduction II. Learning Objectives III. Slides and Micrographs pars intermedia A. General structure B. Parts 1. Anterior Pituitary a. Divisions i. Pars distalis anterior pituitary ii. Pars intermedia iii. Pars tuberalis b. Cells i. Chromophobes ii. Chromophils posterior 1) Acidophils 2) Basophils pituitary 2. Posterior Pituitary a. Divisions i. Infundibulum stalk ii. Pars nervosa b. Cells i. Pituicytes IV. Summary Lab 19 –Pituitary Gland A560 – Fall 2015 Slide 74: Pituitary, Gomori Trichrome I. Introduction II. Learning Objectives III. Slides and Micrographs A. General structure B. Parts 1. Anterior Pituitary a. Divisions posterior i. Pars distalis pituitary ii. Pars intermedia iii. Pars tuberalis b. Cells anterior i. Chromophobes pituitary ii. Chromophils 1) Acidophils 2) Basophils 2. Posterior Pituitary a. Divisions i. Infundibulum stalk ii. Pars nervosa b. Cells i. Pituicytes IV. Summary Lab 19 –Pituitary Gland A560 – Fall 2015 Slide 128 (Hi‐Res): Pituitary, H&E

I. Introduction II. Learning Objectives III. Slides and Micrographs pars tuberalis A. General structure B. Parts 1. Anterior Pituitary a. Divisions i. Pars distalis ii. Pars intermedia anterior iii. Pars tuberalis pituitary b. Cells i. Chromophobes ii. Chromophils pars 1) Acidophils 2) Basophils distalis 2. Posterior Pituitary posterior a. Divisions pars pituitary i. Infundibulum stalk intermedia ii. Pars nervosa b. Cells i. Pituicytes IV. Summary Lab 19 –Pituitary Gland A560 – Fall 2015 Slide 122: Pituitary, H&E I. Introduction II. Learning Objectives III. Slides and Micrographs A. General structure B. Parts 1. Anterior Pituitary a. Divisions i. Pars distalis ii. Pars intermedia iii. Pars tuberalis b. Cells i. Chromophobes ii. Chromophils 1) Acidophils 2) Basophils 2. Posterior Pituitary a. Divisions i. Infundibulum stalk ii. Pars nervosa b. Cells i. Pituicytes the pars distalis accounts for 75% of the anterior pituitary (adenohypophysis); it is composed primarily of IV. Summary cords of endocrine cells interspersed with fenestrated capillaries; some reticular CT is also present Lab 19 –Pituitary Gland A560 – Fall 2015 Slide 128 (Hi‐Res): Pituitary, H&E I. Introduction II. Learning Objectives III. Slides and Micrographs A. General structure B. Parts 1. Anterior Pituitary anterior a. Divisions pituitary i. Pars distalis ii. Pars intermedia iii. Pars tuberalis b. Cells i. Chromophobes ii. Chromophils pars 1) Acidophils intermedia 2) Basophils 2. Posterior Pituitary a. Divisions i. Infundibulum stalk posterior ii. Pars nervosa pituitary b. Cells i. Pituicytes the pars intermedia contains basophilic cells between the pars distalis of the anterior pituitary and the IV. Summary pars nervosa of the posterior pituitary; eosinophilic colloid‐filled cysts represent remnants of the hypophyseal pouch (Rathke’s pouch); functional significance of this region is uncertain Lab 19 –Pituitary Gland A560 – Fall 2015 Slide 74: Pituitary, Gomori Trichrome I. Introduction II. Learning Objectives III. Slides and Micrographs A. General structure B. Parts Look here for secretory cells 1. Anterior Pituitary a. Divisions i. Pars distalis ii. Pars intermedia posterior iii. Pars tuberalis pituitary b. Cells anterior i. Chromophobes pituitary ii. Chromophils 1) Acidophils 2) Basophils 2. Posterior Pituitary a. Divisions i. Infundibulum stalk ii. Pars nervosa b. Cells i. Pituicytes IV. Summary Lab 19 –Pituitary Gland A560 – Fall 2015 Slide 74: Pituitary, Gomori Trichrome I. Introduction II. Learning Objectives III. Slides and Micrographs A. General structure B. Parts 1. Anterior Pituitary a. Divisions acidophil i. Pars distalis ii. Pars intermedia iii. Pars tuberalis b. Cells i. Chromophobes ii. Chromophils 1) Acidophils 2) Basophils 2. Posterior Pituitary a. Divisions i. Infundibulum stalk ii. Pars nervosa b. Cells i. Pituicytes IV. Summary basophil chromophobe Lab 19 –Pituitary Gland A560 – Fall 2015 Slide 122: Pituitary, H&E

I. Introduction II. Learning Objectives III. Slides and Micrographs A. General structure B. Parts 1. Anterior Pituitary a. Divisions i. Pars distalis ii. Pars intermedia iii. Pars tuberalis acidophil basophil b. Cells i. Chromophobes ii. Chromophils 1) Acidophils 2) Basophils 2. Posterior Pituitary a. Divisions i. Infundibulum stalk ii. Pars nervosa b. Cells i. Pituicytes IV. Summary chromophobes are identified by their pale‐staining cytoplasm and absence of secretory granules; they represent several different types of cells, including stem cells and degranulated cells Lab 19 –Pituitary Gland A560 – Fall 2015

I. Introduction Slide 74: Pituitary, Gomori Trichrome II. Learning Objectives III. Slides and Micrographs A. General structure B. Parts 1. Anterior Pituitary a. Divisions i. Pars distalis ii. Pars intermedia iii. Pars tuberalis b. Cells i. Chromophobes ii. Chromophils 1) Acidophils 2) Basophils 2. Posterior Pituitary a. Divisions i. Infundibulum stalk ii. Pars nervosa b. Cells i. Pituicytes IV. Summary acidophils have strongly‐staining acidophilic (eosinophilic) cytoplasm; they secrete growth hormone and prolactin (somatotrophs [about half of total cells] and lactotrophs, respectively) Lab 19 –Pituitary Gland A560 – Fall 2015

I. Introduction Slide 74: Pituitary, Gomori Trichrome II. Learning Objectives III. Slides and Micrographs A. General structure B. Parts 1. Anterior Pituitary a. Divisions i. Pars distalis ii. Pars intermedia iii. Pars tuberalis b. Cells i. Chromophobes ii. Chromophils 1) Acidophils 2) Basophils 2. Posterior Pituitary a. Divisions i. Infundibulum stalk ii. Pars nervosa b. Cells i. Pituicytes IV. Summary basophils (less numerous) have strongly‐staining basophilic cytoplasm; they include: corticotrophs (ACTH and LPH), gonadotrophs (FSH and LH/ICSH), and thyrotrophs (TSH) Lab 19 –Pituitary Gland A560 – Fall 2015 Slide 128 (Hi‐Res): Pituitary, H&E

I. Introduction II. Learning Objectives III. Slides and Micrographs infundibulum stalk A. General structure B. Parts 1. Anterior Pituitary a. Divisions i. Pars distalis ii. Pars intermedia iii. Pars tuberalis anterior b. Cells pituitary i. Chromophobes ii. Chromophils 1) Acidophils 2) Basophils posterior 2. Posterior Pituitary pituitary a. Divisions i. Infundibulum stalk pars ii. Pars nervosa nervosa b. Cells i. Pituicytes IV. Summary Lab 19 –Pituitary Gland A560 – Fall 2015 Slide 128 (Hi‐Res): Pituitary, H&E

I. Introduction II. Learning Objectives III. Slides and Micrographs A. General structure B. Parts 1. Anterior Pituitary a. Divisions i. Pars distalis ii. Pars intermedia iii. Pars tuberalis b. Cells i. Chromophobes ii. Chromophils 1) Acidophils 2) Basophils 2. Posterior Pituitary a. Divisions i. Infundibulum stalk ii. Pars nervosa b. Cells i. Pituicytes pituicytes, majority of nuclei seen, are glial cells that support the unmyelinated axons that compose majority IV. Summary of posterior pituitary (neurohypophysis); axons release ADH and oxytocin (nothing produced in posterior pituitary); , faintly‐staining eosinophilic accumulations of secretory products in axon terminals (not to be confused with capillaries), might be seen Lab 19 –Pituitary Gland A560 – Fall 2015

I. Introduction II. Learning Objectives III. Slides and Micrographs EMs to Examine A. General structure B. Parts 1. Anterior Pituitary a. Divisions Fig 20‐7: Ultrastructure of somatotropic cells i. Pars distalis ii. Pars intermedia iii. Pars tuberalis b. Cells i. Chromophobes ii. Chromophils 1) Acidophils 2) Basophils 2. Posterior Pituitary a. Divisions i. Infundibulum stalk ii. Pars nervosa b. Cells i. Pituicytes IV. Summary