Polyclonal Antibody to Serotonin Receptor 3A (HTR3A) (21-36) - Aff - Purified

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AP08449PU-N Polyclonal Antibody to Serotonin receptor 3A (HTR3A) (21-36) - Aff - Purified

Alternate names: 5-HT3A, 5-HT3R, 5-hydroxytryptamine receptor 3A, Serotonin-gated ion channel receptor Quantity: 50 µg Concentration: 1 mg/ml Background: Serotonin (5-hydroxytryptamine, 5-HT), originally discovered as a serum factor plays important roles in regulating diverse biological processes in central and peripheral nervous systems, cardiovascular systems, and gastrointestinal systems. The 5-HT3 receptors are distinguished from other 5-HT receptors by their structure and function. 5-HT3 receptors span the membrane only four times and are ligand-gated, cation- selective ion channels that mediate neuronal excitation and exocytotic release of neurotransmitters within the peripheral and central nervous systems. Functional studies done on isolated tissues have revealed heterogeneity of this receptor. The human 5-HT3R-As cDNA contains an open reading frame of 1434 base pairs predicting a 23-residue signal peptide and a mature receptor subunit of 455 amino acids. The human subunit exhibits 85% and 84% amino acid sequence identity to the mouse and rat receptors, respectively. Anti-5-HT3R polyclonal antibody shows immunoreactivity in the forebrain (olfactory regions, hippocampal formation and amygdala), sensory and motor nuclei of brainstem and spinal cord. Uniprot ID: P46098 NCBI: NP_000860.2 GeneID: 3359 Host / Isotype: Rabbit / IgG Immunogen: Synthetic peptide corresponding to amino acids 21-36 (RATQAHSTTQPALLRL) and 421-436 (LSSIRHSLEKRDEMRE) of Rat 5-HT3 Receptor. Remarks: Percent identity by BLAST analysis: Rat (100%); Mouse (88%); Hamster (81%). Format: State: Liquid purified Ig fraction. Purification: Protein G Chromatography. Buffer System: PBS, 0.05% sodium azide. Applications: Immunohistochemistry on Paraffin Sections: 5 µg/ml. Western Blot: 1 µg/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: This antibody recognizes 5-Ht3a Receptor (HTR3A). In Human brain, a 47-50 kD band is observed. Species Reactivity: Tested: Human. Expected from sequence similarity: Rat.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20170919 1 / 2 AP08449PU-N: Polyclonal Antibody to Serotonin receptor 3A (HTR3A) (21-36) - Aff - Purified

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Pictures: Anti-5HT3A receptor antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot of 5-HT3R in human brain tissue lysate with anti-5-HT3R pcAb. A protein band of approximate molecular weight of 47-50 kDa was detected.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20170919 2 / 2