Nautilus Report
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Results of Sediment Toxicity Testing for Bight 18 - Santa Margarita River Estuary Sample IDs: B18-10167 and B18-10166 Monitoring Period: August 2018 Prepared for: Weston Solutions, Inc. 5817 Dryden Place, Suite 101 Carlsbad, CA 92008 Prepared by: Nautilus Environmental 4340 Vandever Avenue San Diego, CA 92120 (858) 587-7333 Date Submitted: December 11, 2018 Data Quality Assurance: o Nautilus Environmental is accredited in accordance with NELAP by the State of Oregon Environmental Laboratory Accreditation Program (Certificate No. 4053). It is also certified by the State of California Department of Health Services Environmental Laboratory Accreditation Program (Certificate No. 1802) and the State of Washington Department of Ecology (Lab ID C552). o All data have been reviewed and verified. All test results have met minimum test acceptability criteria under their respective o EPA protocols, unless otherwise noted in this report. o All test results have met internal Quality Assurance Program requirements. Verified by: California 4340 Vandever Avenue San Diego, California 92120 858.587.7333 Kasey Skrivseth, Project Manager fax: 858.587.3961 Toxicity Testing Report for Bight 18 - Santa Margarita River Estuary Weston Solutions, Inc Test IDs: 1809-S018 to S021 Monitoring Period: August 2018 TABLE OF CONTENTS 1.0 INTRODUCTION ............................................................................................................1 2.0 MATERIALS AND METHODS ........................................................................................... 1 2.1 Sample Collection, Receipt, and Preparation ............................................................. 1 2.2 Toxicity Test Methodology ......................................................................................... 1 2.3 Statistical Analyses ....................................................................................................4 2.4 Testing Schedule .......................................................................................................4 3.0 RESULTS ....................................................................................................................4 3.1 Solid-Phase Toxicity Tests ......................................................................................... 5 3.2 Sediment-Water Interface Toxicity Tests ................................................................... 5 4.0 QUALITY ASSURANCE ..................................................................................................6 4.1 Sample Receipt, Handling and Holding Time ............................................................. 6 4.2 Solid-Phase Toxicity Tests ......................................................................................... 6 4.3 Sediment-Water Interface Tests ................................................................................ 6 4.4 Reference Toxicant Tests .......................................................................................... 6 5.0 REFERENCES ..............................................................................................................7 Nautilus Environmental, San Diego, CA i Toxicity Testing Report for Bight 18 - Santa Margarita River Estuary Weston Solutions, Inc Test IDs: 1809-S018 to S021 Monitoring Period: August 2018 LIST OF TABLES Table 1. Toxicity Test Methodology and QA/QC Requirements for the 10-day Solid Phase Amphipod Toxicity Test .................................................................................. 2 Table 2. Toxicity Test Methodology and QA/QC Requirements for Sediment Water Interface Bivalve Larvae Development Toxicity Tests ................................................ 3 Table 3. Toxicity Testing Schedule .......................................................................................... 4 Table 4. Summary of Amphipod Solid-Phase Toxicity Test Results ......................................... 5 Table 5. Summary of Mussel Sediment-Water Interface Toxicity Test Results ........................ 5 Table 7. Total and Un-ionized Ammonia Concentrations in Bulk Sediment - Sediment Porewater ................................................................................................. Appendix D Table 8. Total and Un-ionized Ammonia Concentrations in Solid-Phase Amphipod Toxicity Tests – Overlying Water .............................................................. Appendix D Table 9. Total and Un-ionized Ammonia Concentrations in Sediment-Water Interface Toxicity Tests – Overlying Water .............................................................. Appendix D LIS T OF APPENDICES Appendix A: Chain-of-Custody Forms and Sample Check-in Information Appendix B: Statistical Analyses and Raw Data Packages for Eohaustorius Appendix C: Statistical Analyses and Raw Data Packages for Mytilus Appendix D: Ammonia tables and Raw Data Packages Appendix E: Reference Toxicant Raw Data and Analyses Appendix F: List of Laboratory Qualifier Codes Nautilus Environmental, San Diego, CA ii Toxicity Testing Report for Bight 18 - Santa Margarita River Estuary Weston Solutions, Inc Test IDs: 1809-S018 to S021 Monitoring Period: August 2018 1.0 INTRODUCTION Weston Solutions, Inc. (Weston) has partnered with Nautilus Environmental (Nautilus) to perform sediment toxicity testing as part of the Santa Margarita River Estuary monitoring portion of the Southern California Bight 2018 Regional Marine Monitoring Survey (Bight’18) within the counties of San Diego and Riverside, which is coordinated by the Southern California Coastal Water Research Project (SCCWRP). Specific testing guidelines provided by SCCWRP for Bight’18 as well as the Sediment Quality Objectives (SQO) program were followed for this testing event (SCCWRP 2018 and 2009). This report summarizes results of the August 2018 sampling event. Two sediment samples were collected and evaluated using two test methods. These included: • 10-day amphipod Eohaustorius estuarius (Eohaustorius) solid-phase test (SCCWRP 2018 and 2009; USEPA 1994) • 48-hour mussel Mytilus galloprovincialis (Mytilus) larvae development sediment-water interface (SWI) test (SCCWRP 2018 and 2009; Anderson et al., 1996) 2.0 MATERIALS AND METHODS The methods employed to evaluate sediment toxicity are described below. 2.1 Sample Collection, Receipt, and Preparation Test site sediments for the Bight'18 program were collected on August 29, 2018 by Weston personnel. Approximately six to eight liters of sediment were provided from each site, in one double layered high density polyethylene plastic bag. Samples were delivered in coolers with wet ice to Nautilus by Weston personnel on August 30, 2018. Upon receipt, contents of coolers were verified against chain of custody forms. Temperatures were measured and recorded on a sample check-in form. All samples were stored at 4 ± 2°C in the dark until used for testing. Copies of chain of custody forms and sample check-in sheets are provided in Appendix A. For all testing, an aliquot of each sample was sieved through a 1.0-mm Nitex® mesh screen to remove potential native organisms and ensure reliable test organism recovery. Following homogenization, interstitial pore water samples were collected and analyzed for total ammonia content. Prior to testing, each sample was thoroughly homogenized. 2.2 Toxicity Test Methodology Test methods and acceptability criteria for the 10-day amphipod and the mussel SWI are described in Tables 1 and 2, respectively. Nautilus Environmental, San Diego, CA 1 Toxicity Testing Report for Bight 18 - Santa Margarita River Estuary Weston Solutions, Inc Test IDs: 1809-S018 to S021 Monitoring Period: August 2018 Table 1. Toxicity Test Methodology and QA/QC Requirements for the 10-day Solid Phase Amphipod Toxicity Test Test organism Marine Amphipod – Eohaustorius estuarius Test organism source Northwest Aquatic Sciences; Newport, OR Test organism size at initiation 3-5 mm Test duration; endpoint 10 days; survival Test solution renewal None Feeding Prior to test initiation only Test chamber 1-L glass jar Sediment depth 2 cm Overlying water volume 800 mL Test temperature 15 ± 1°C test-wide mean, 15 ± 3°C instantaneous Overlying water quality monitoring pH, temperature, salinity, and dissolved oxygen daily, total ammonia on days 0 and 10 Overlying water Natural seawater collected offshore SIO Pier in La Jolla, CA. 20-µm filtered. Seawater was diluted to 17 or 32 ppt with deionized water prior to testing. Number of organisms per chamber 20 5, plus 1 surrogate test chamber for water quality Number of replicates readings Sieved sand collected near organism home site Negative controls (Lab Control #1 32 ppt) Photoperiod Continuous light (24 hour) Aeration Continuous (3-4 bubbles per second) SCCWRP 2018, 2009, and USEPA 600/R-94/025 Test Protocol (EPA 1994) Test acceptability criteria ≥ 90 percent mean survival in controls Reference toxicant Ammonium chloride cm – centimeter °C – degrees Celsius L - Liter mL – milliliter mm - millimeter ppt – parts per thousand SIO – Scripps Institution of Oceanography µm - micrometer USEPA – United States Environmental Protection Agency Nautilus Environmental, San Diego, CA 2 Toxicity Testing Report for Bight 18 - Santa Margarita River Estuary Weston Solutions, Inc Test IDs: 1809-S018 to S021 Monitoring Period: August 2018 Table 2. Toxicity Test Methodology and QA/QC Requirements for Sediment Water Interface Bivalve Larvae Development Toxicity Tests Test organism Mediterranean mussel - Mytilus galloprovincialis Test organism source Field collected locally;