229 2 R A EHRHARDT and others Leptin action in early lactating 229:2 145–157 Research dairy cows Increased plasma leptin attenuates adaptive metabolism in early lactating dairy cows Richard A Ehrhardt1, Andreas Foskolos2, Sarah L Giesy3, Stephanie R Wesolowski4, Christopher S Krumm3, W Ronald Butler3, Susan M Quirk3, Matthew R Waldron3 and Yves R Boisclair3 1Departments of Animal Science and Large Animal Clinical Sciences, Michigan State University, Correspondence East Lansing, Michigan, USA should be addressed 2Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, UK to Y Boisclair 3Department of Animal Science, Cornell University, Ithaca, New York, USA Email 4University of Colorado School of Medicine, Aurora, Colorado, USA
[email protected] Abstract Mammals meet the increased nutritional demands of lactation through a combination Key Words of increased feed intake and a collection of adaptations known as adaptive metabolism f glucose metabolism (e.g., glucose sparing via insulin resistance, mobilization of endogenous reserves, and f lipid metabolism Endocrinology increased metabolic efficiency via reduced thyroid hormones). In the modern dairy f liver of cow, adaptive metabolism predominates over increased feed intake at the onset of f thyroid hormone lactation and develops concurrently with a reduction in plasma leptin. To address the Journal role of leptin in the adaptive metabolism of early lactation, we asked which adaptations could be countered by a constant 96-h intravenous infusion of human leptin (hLeptin) starting on day 8 of lactation. Compared to saline infusion (Control), hLeptin did not alter energy intake or milk energy output but caused a modest increase in body weight loss.