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Beauveria bassiana (Bals.) Vuil. Cydia pomonella (L.) ! "## $ %&

1 2 1 1 765 . (1) . ! (2)

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.& +/ &, & .Cydia pomonella (L.) )'$ * +, - '$ bassiana (Bals.) Vuil. ' .1060102 :24

! . Cydia pomonella (L.) (B A) Beauveria bassiana (Bals.) Vuil 0' 123 $ , ./º 28-24 & & ' $ , B. bassiana B A % $ & ' *+! ! "#$ 96.6 % ! 10 % # :$ ; 7& 9 6 7 8 % *4 5

+ $% B % ! ' , . 8 9B A % 6 %93.3 93.3 7 %93.3 .% 12 % ! 10 % %90 # :$ ; 2 9 .% 9& ' 9& &+, :

$ .(11) -? K * K ;21 / ) $ ! :) >/$ ) 6 5) -2 Protenases - / M KC L" K+1 !" #$ .(1) Lipases & KC $ Beauveria bassiana (Bals.) Vuil %&$ 32 -K/ 8 * B 5% Verticillium lecanii anisopliae (Metsch.) Sorokin. 2 :A ?" *+ N" !) @ A ! '(& ) .Nomaraea rileyi (Zimmerman) %2 5% N2 '(% C !4 .1 +, -/ " 0( " *+ . +7 " +, / *+ NO4 / *+ %2 -) -" '(& $ 1 -2 -" 34 5% -, 1 +, %&$ %2 / ) -, 6 * , )'$ * & +' 1#$ % " . +7 " *+ 4 %

* @ A ! ? 4 P( ") 8/ - 6( 1 +, + 4 *+ B. bassiana -O2 H - 4 + %/ & :2 .) ) 9 " !/

4/ :A N) > ? 9 .2001/2000 6 : Psedophilus 4 ( -2 ; +, (& ! %10 K, (NaOCl) : ,& - Bemisia tabaci > ( (3) testaceus (Gahan) 5) :4" Q ; 8/ %4" " ? " .;? 5 $ 8 $ ? .(6) )( 8/ (Gennadins) Q , %) R ? $ $ BOA 8 % -, "? @ A ! ? *+ / -? B$ B. bassiana :4" 6 ; -, "? 8 "? ? 4 /K .4" :$ 10D8 " - ? C Cydia pomonella (L.) /T –K, –)S *(H - 20 8/ 6 2 8/ > ( +, E2 $ .(15) "

Tº 121 ! ) / :4" K%) :4" (PSA) +7 '(% H 9 %90 -? 8 :/! 3 ) / ; :A 4? 20 ! :/U, 15 H ; / B. bassiana / : .(17 16) 4 :$ AOA " ; + .Tº 1±27 ! '(& J ! ! $ :) ) ; * I$ .(H 8/ /K 4 - / 4 9 / 2 ) %?2 :A)

(2006) 2 24 102 67 $ 5$ 6$ ;2 / , -? 2

-" CRD -, *" : ;+ R) ( : %4 " .?" ( ? /* 4 0.05 - / (LSD) 6" ;+ -? 2 / /T –K, –)S *(H 8/ %2 .X ( " %5±60 Tº 1±27 ! ) .R) 4 Beauveria bassiana ' / - + *2$ $ )'$ * & +' 1#$ % 28 26 24 18 10) 2 ! ) A T ! 4+ Q / -O2 B A K" !" 8/ (Tº 1±35 * R & B. bassiana @ A ! –K, – )S *(H 8/ K/ -, /K .B. bassiana H ( -4 /) ? -K/ 6( :A , T2 ))K ;$ -2 /T 6 : -O2 ? #) %9.8 ') &" : :$ " ! ) / ; 32 -K" X %$ .(5) 2001/2000 .K" O, , -, *+ !" ? T? 8/ (& ( ) 2 K/ ) B A B. bassiana *+ E/ - B. bassiana 32 " .! ? * +, - B. bassiana ' &3 ( $ 8/ ') 32 $ ; ( N @ A ! 8/ ;" 4$ )'$ 4 ( -2 ; (Pseudophilus testaceus) ! - ? @ A -4 ) .(4) Tº 1±28 ! ) / N2 %" : @ A

(& ? V/ . BA 8 % Beauveria bassiana ' +7% / - + *2$ *+ Q A ! ) X %$ B A K" 6 ;" - 5 W @ A ! 6 ! ) Y .(1 -)) K" / N" !" -/I 10 K, B. bassiana+ 0.2 0.5 !" ? U ( 2 :2 K, R : B (- 50) :) !H Tº 10 ! ) / /K Z :$ " " : ? 8/ ; " .(14) Haemocytometer /$ -) : Tº 1±26 ! ) / *+ " ? K *+ .* 8/ B A K" -, -+$ , ! ) !K K" O, !" ) , .:$ 10 7 3 " " -, *+ ? @ ( Tº 28 D24 ! ) / K" O, K/ -, -, ? 10 , -, : BOA .& " / NO+ 7.3 6.0 A K" : 6.8 6.0 !" ?

5" + (Tº 35) " ! ) / $ .B K" : &$#$ +, - B. bassiana ' &3 ( $ !" ? U B K" O, !" 4$ )'$ * K" : 0.1 K" : 2.4 :$ 7 " B N4 /) @ A ! ? / / K" O, !" 5" O :4 . A - / I 106 K, 6 ;" - 5 W A, )$ B A K" -+$ $ 2 " ! ) ? .4+ B. bassiana B K" -+$ B+ B K" $ Tº 24 ! ) *+ / ? >41 0( Tº 1±18 ! ) / *+ " .Tº 28 ! ) / %+ 7 3 " ? / R : . *+ "

? 10 , -, : BOA ) , .:$ 10 )'$ * +, - ' &3 ( B. bassiana .& " / NO+ 4$ !/ ; / 4 R) *+ # , : " / :) H B. bassiana –)S *(H 8/ " " ? >K)$ #K K" %30 26.6 BA ? " :$ AOA . / 32 /T –K, , .(2 -)) % " ;+ * 8/ B A " :$ 3 " ?

103 Arab J. Pl. Prot. Vol. 24, No. 2 (2006) I C %92.5 A,$ Tº 35 D15 " ;+ ) B K" %33.3 A K" %20 -A $ ,[( *+ .6 -KH .(2 -)) %

" -O2 / 8 P V. lecanii B. bassiana

.(13) 1 B I C :$ '* & B A & $% "# .1 0(, O ! ) + $ ) :4 Beauveria bassiana (Bals.) :) %?2 I 1 % *" Table 1. Effect of temperature on the growth of isolates A and B of Beauveria bassiana (Bals.). K O $ .(10) N4 ) (& ! 7 () 1 $ T$ 8/ $ ,+ ? Fungal colony diameter (cm.) A % R 6 B1 K ! 8 P after 7 days (1 ± °) / ! ) ; N9$ X .! A B Temperature Isolate A Isolate B (°C ± 1) / 2 K$ K $ ,+ O + 0.5 0.2 10 & , - :4 ) O 4 3.1 2.8 18 6.8 6.0 24 E?2 8/ 1 $ / :) ) R, *+ 2 6.2 7.0 26 2 >/ R 8/ ! :) -2 6.0 7.3 28 0.1 2.4 35 (& -" T ? .(4) ! 8 6M

B T *+ @ @ A ! +, X " ? *+ # +7 '(& $ *+ B. bassiana "+ 8 " ;+ ) :/ 8 X $ K" O, ? - " ? A,$ 2 " ? -" , ? 8/ A B B. bassiana *K/ ? *+ B. bassiana A $ ) *+ .(12) .(2 -)) BA A,$ , ;" *+ !( ; 8 Sesamia cretica Led. E A B. bassiana *K" $ X %$ ! 2 ? 4 *+ 'A *+ @ A ! BA ? *+ -"+ , .(2) +7 '(& +, X *+ E" ) &/ )$ * ! ) 8 0( " ? .2 @ ; ? *+ $ ) , . " ? " B *+ , A % , * " L" !+ !K K ( ) Zeuzera pyrina Scolytus scolytus >2 ? ) ) 4 *+ +, *+ :2 , .(7) B. bassiana B. bassiana ! ) / B. bassiana 1 B / Pseudophilus ;4 ( -2 ; 8A ! ) $ Tº 30D5 *+ ? -" # 8 $ testaceus .(9) Tº 21.6 , 1978 */ *+ %94.3 95.4 8 " -2 )$ -" N$ + " 2$ %) .( ) * 8/ 1980 3 (18) 2S Walstad ,$ 4+ . /+ *+ AM * @ ! ) 8 P B. bassiana $

.Cydia pomonella (L.) +* " , " &' $% Beauveria bassiana (Bals.) '* $ "# .2 Table 2. Effect of Beauveria bassiana (Bals.) isolates on 3rd and 4th larval instars of the , Cydia pomonella (L.).

Percentage of dead larvae th # rd !" 4 instar 3 instar 10 7 3 10 7 3 $

after 10 days after 7 days after 3 days After 10 days after 7 days after 3 days Treatments 6.6 3.3 0.0 3.3 3.3 3.3 Control -/ 93.3 63.3 20.0 96.6 70.0 26.6 Isolate A A 93.3 66.6 33.3 93.3 76.6 30.0 Isolate B B 8.15 = ! 4.6 = 4.6 = 0.05 LSD at P= 0.05 for treatments = 4.6, for days = 4.6, and for treatments × days = 8.51

(2006) 2 24 104 $% (B ) Beauveria bassiana (Bals.) '* "# .3 &$#$ +, - B. bassiana ' &3 ( $ .Cydia pomonella +* " / (L.) 4$ )'$ * Table 3. Effect of Beauveria bassiana (Bals.) on the hibernating larvae of codling moth, Cydia pomonella (L.). -"+ , -, @ A ! ? A

H 4+ (3 -)) B. bassiana B K" Percentages of dead larvae '(& " %16.66 " :$ 3 " ? 10 7 3 $ 4+ .& " 4 " ; ? after 10 after 7 after 3 $ days days days Treatments ? *+ / R - 8 (8) Audemard 10.00 03.33 00.00 Control 0 *+ B. bassiana %+, / @ A ! / 4 X '(& :/ 6( + *+ R) 90.00 56.66 16.66 Isolate B B 0( .) 5? !) ? 8/ ) = 7.26 = %5 0( ? +, (& -" * $ , .8.89 LSD at P=0.05 for treatments = 7.26 and for days = 8.89. 6 ;" A, @ ) ; " . : >A +7 " L2 8 6M > : -O2

Abstract Khlaywi, S.A., H.F Alrubaei, I.J. Al-Jboory and Sh.A. Al-Tai. 2006. Laboratory Evaluation for Efficacy of two Isolates of Beauveria bassiana on Parasitizing third and forth Instars Larvae of the Cydia pomonella (L.). Arab J. Pl. Prot. 24: 102-106. Two isolates of Beauveria bassiana (Bals.) vuil. (A and B) were obtained from infected larval of Cydia pomonella (L.). Lab rotary results showed that the optimum growth temperature for both isolates A and B ranged from 24-28C0. Pathogenic potential for isolates A and B using 3rd & 4th larval instars of codling moth, Cydia pomonella (L.) resulted in mortalities of 96.6 and 93.3% for 3rd instar and 93.3 and 93.3% for 4th instar, 10 days after treatment, respectively. It was found that isolate B had a significant impact on the mortality of hibernating larvae, and reached 96% 10 days after treatment. Key words: Biological control, temperature, Iraq. Corresponding author: Samira Khlaywi, Science and Technology Research Center, P.O. Box 765, Baghdad, Iraq, Email: [email protected]

References

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Received: August 1, 2004; Accepted: April 25, 2006 2006/4/25 : 2004/8/1 :

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