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2008.003P (To Be Completed by ICTV Officers)

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2008.003P (To Be Completed by ICTV Officers) Taxonomic proposal to the ICTV Executive Committee This form should be used for all taxonomic proposals. Please complete all those modules that are applicable (and then delete the unwanted sections). Code(s) assigned: 2008.003P (to be completed by ICTV officers) Short title: 1 new species in the genus Foveavirus (e.g. 6 new species in the genus Zetavirus; re-classification of the family Zetaviridae etc.) Modules attached 1 2 3 4 5 (please check all that apply): 6 7 Author(s) with e-mail address(es) of the proposer: Mike Adams ([email protected]) on behalf of the Flexiviridae SG ICTV-EC or Study Group comments and response of the proposer: MODULE 5: NEW SPECIES Code 2008.003P (assigned by ICTV officers) To create 1 new species assigned as follows: Fill in all that apply. Ideally, species Genus: Foveavirus should be placed within a genus, but it is Subfamily: acceptable to propose a species that is Family: proposed family Betaflexiviridae within a Subfamily or Family but not (formerly Flexiviridae) assigned to an existing genus (in which case put “unassigned” in the genus box) Order: Name(s) of proposed new species: Peach chlorotic mottle virus Argument to justify the creation of the new species: If the species are to be assigned to an existing genus, list the criteria for species demarcation and explain how the proposed members meet these criteria. Species demarcation criteria published in the 8th report are: Each distinct species usually has a specific natural host range. Distinct species do not cross-protect in infected common host plant species. Distinct species are readily differentiated by serological procedures; strains of individual species are often distinguishable in reactions with polyclonal antisera, but more readily so with monoclonal antibodies. Distinct species have less than ca. 72% identical nt or 80% identical aa between their CP or polymerase genes. The virus isolated from peach cross-reacts with antisera to diverse viruses, such as plum pox virus Page 1 of 3 Taxonomic proposal to the ICTV Executive Committee Argument to justify the creation of the new species: (PPV), genus Potyvirus, family Potyviridae; and apple stem pitting virus (ASPV), genus Foveavirus, family Flexiviridae. The PCMV genome (EF693898) consists of 9005 nucleotides and has an organisation similar to that of ASPV but the two genomes share a nucleotide (nt) sequence identity of only 58%. Despite the serological cross reaction, the amino acid sequences of the CPs of PCMV and ASPV CP share only 37% identity (James et al., 2007). Phylogenetic analyses (Fig. 1) and sequence comparisons justify its status as a distinct species within the genus Foveavirus (it has 55% aa identity to ASPV in its polymerase sequence) Fig. 1. Phylogenetic (NJ) tree of the amino acid sequences of the polymerase (ORF1) of members of the genera Carlavirus and Foveavirus with an unassigned member of the family, Sugarcane streak mosaic associated virus, as an outgroup. JTT distances and 100 bootstrap replicates. From MEGA4 97 ab032469 HpLV 88 eu527979 HpMV 100 ab051848 AcLV am158439 NCLV 100 100 d14449 PVM 91 dq098905 NeLV ab245142 CVB 98 100 aj620300 DVS 93 eu020009 PVP Carlavirus aj863509 PVS 100 eu074853 LiNRSV ay941198 BlScV 100 69 aj516059 LSV 100 dq455582 PaLV ef527260 CVNV 99 ay505475 PopMV z68502 SLV ay461421 SPCFV af026278 GRSPaV 100 d21829 ASPV Foveavirus 100 ef693898 PcCMV af315308 SSMaV 0.2 References: James D., Varga A., Croft H. (2007). Analysis of the complete genome of peach chlorotic mottle virus: identification of non-AUG start codons, in vitro coat protein expression, and elucidation of serological cross-reactions. Arch. Virol. 152:2207-2215 Page 2 of 3 Taxonomic proposal to the ICTV Executive Committee Page 3 of 3.
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