COMPARATIVE A peer-reviewed open-access journal CompCytogen 11(4): 641–657 (2017) A chromosomal analysis of Nepidae 641 doi: 10.3897/CompCytogen.v11i4.14928 RESEARCH ARTICLE Cytogenetics http://compcytogen.pensoft.net International Journal of Plant & Animal Cytogenetics, Karyosystematics, and Molecular Systematics A chromosomal analysis of Nepa cinerea Linnaeus, 1758 and Ranatra linearis (Linnaeus, 1758) (Heteroptera, Nepidae) Robert B. Angus1, Constance Jeangirard2, Desislava Stoianova3, Snejana Grozeva3, Valentina G. Kuznetsova4 1 Department of Life Sciences (Insects), The Natural History Museum, Cromwell Road, London SW7 5BD, UK 2 School of Biological Sciences, Royal Holloway University of London, Egham, Surrey TW20 0EX, UK 3 Institu- te of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 1 Tsar Osvoboditel, Sofia 1000, Bulga- ria 4 Zoological Institute, Russian Academy of Sciences, Universitetskaya nab. 1, St. Petersburg 199034, Russia Corresponding author: Robert B. Angus (
[email protected]) Academic editor: S. Nokkala | Received 7 June 2017 | Accepted 13 August 2017 | Published 14 September 2017 http://zoobank.org/AF03D8C6-E9BC-4135-884C-5BF9791FD78B Citation: Angus RB, Jeangirard C, Stoianova D, Grozeva S, Kuznetsova VG (2017) A chromosomal analysis of Nepa cinerea Linnaeus, 1758 and Ranatra linearis (Linnaeus, 1758) (Heteroptera, Nepidae). Comparative Cytogenetics 11(4): 641–657. https://doi.org/10.3897/CompCytogen.v11i4.14928 Abstract An account is given of the karyotypes and male meiosis of the Water Scorpion Nepa cinerea Linnaeus, 1758 and the Water Stick Insect Ranatra linearis (Linnaeus, 1758) (Heteroptera, Nepomorpha, Nepidae). A number of different approaches and techniques were tried: the employment of both male and female gonads and mid-guts as the sources of chromosomes, squash and air-drying methods for chromosome preparations, C-banding and fluorescencein situ hybridization (FISH) for chromosome study.