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Metabolic Characterization of <I>Bacillus</I>
1605 Journal of Food Protection, Vol. 77, No. 9, 2014, Pages 1605–1611 doi:10.4315/0362-028X.JFP-14-145 Copyright G, International Association for Food Protection Research Note Metabolic Characterization of Bacillus subtilis and Bacillus amyloliquefaciens Strains Isolated from Traditional Dry-Cured Sausages AIDA CACHALDORA, SONIA FONSECA, MARI´AGO´ MEZ, INMACULADA FRANCO, AND JAVIER CARBALLO* Downloaded from http://meridian.allenpress.com/jfp/article-pdf/77/9/1605/1687240/0362-028x_jfp-14-145.pdf by guest on 26 September 2021 A´ rea de Tecnologı´a de los Alimentos, Facultad de Ciencias de Ourense, Universidad de Vigo, 32004 Ourense, Spain MS 14-145: Received 27 March 2014/Accepted 29 April 2014 ABSTRACT The aim of this study was to investigate the effect of pH, temperature, and NaCl on growth, proteolytic and lipolytic activities, and the ability to produce biogenic amines of 19 strains of Bacillus isolated from Androlla and Botillo (two Spanish traditional sausages) to elucidate the role of these bacteria in sausage manufacture. All strains grew in the presence of 10% salt and at pH values of 5.0 and 5.5, whereas only 9 strains grew at 10uC. Proteolytic activity was assessed by the agar plate method, which revealed that 100 and 94.7% of the strains were able to hydrolyze sarcoplasmic and myofibrillar proteins, respectively. These results were confirmed by electrophoretic assays. The titration method revealed that only two strains hydrolyzed pork fat to any extent, and the profiles of the fatty acids freed were different. Most strains produced biogenic amines, but the quantities were generally low. -
Physicochemical, Hygienic and Organoleptic Characterization Of
Physicochemical, hygienic and organoleptic characterization of Slavonian kulen Physicochemical, hygienic and organoleptic characterization of Slavonian kulen ;/"/457&/04536ė/*%*0 ance, surface smell, consistency, in- Physicochemical, hygienic ner smell, cross section quality, tex- ture, taste and aroma and aftertaste. and organoleptic characterization Based on mean score of the particu- lar organoleptic characteristic and of Slavonian kulen the coefficient of importance for that particular attribute, the overall qual- ity was calculated by the following Karolyi, D. 1 formula: scientific paper 0WFSBMMRVBMJUZY B C D E F G H I Summary where a, b, c, d, e, f, g and h are the Slavonian kulen (SK) is traditional dry sausage produced in the region of Slavonia in eastern Croatia. It is made from mince of pork, back fat, spices and salt filled into pork cecum. After stuffing, the sausages are cold-smoked and ripened-dried afterward for several mean scores of evaluator’s assess- months. Present work aimed to analyse some physicochemical and organoleptic traits of ripe SK as well as the safety of final product. ments for: surface appearance, sur- The SK samples (n=12) from different small-scale manufactures were analysed. The following physicochemical attributes (mean ± face smell, consistency, inner smell, s.d.) were recorded: moisture 38.2%±3.6, protein 35.0%±3.1, fat 23.7%±4.6, moisture/protein ratio 1.1±0.1, pH value 5.37±0.23 and cross section quality, texture, taste water activity (aw) 0.82±0.02. Mean organoleptic scores, on five-point scale, were 3.7±0.6 for surface appearance, 3.4±0.6 for surface and aroma, and after-taste, respec- smell, 3.8±0.5 for consistency, 3.2±0.4 for inner smell, 3.0±0.7 for cross section quality, 3.3±0.5 for texture, 3.1±0.4 for taste and aroma, 3.0±0.5 for after taste and 3.2±0.4 for overall quality. -
Distribution of Polycyclic Aromatic Hydrocarbons in Traditional Dry Cured Smoked Ham Slavonska Šunka
applied sciences Article Distribution of Polycyclic Aromatic Hydrocarbons in Traditional Dry Cured Smoked Ham Slavonska Šunka Krešimir Mastanjevi´c 1,* , Brankica Kartalovi´c 2 , Jasmina Lukinac 1 , Marko Juki´c 1 , Dragan Kovaˇcevi´c 1, Jelena Petrovi´c 2 and Kristina Habschied 1 1 Faculty of Food Technology Osijek, Josip Juraj Strossmayer University of Osijek, F. Kuhaˇca20, 31000 Osijek, Croatia; [email protected] (J.L.); [email protected] (M.J.); [email protected] (D.K.); [email protected] (K.H.) 2 Scientific Veterinary Institute Novi Sad, Rumenaˇckiput 20, 21000 Novi Sad, Serbia; [email protected] (B.K.); [email protected] (J.P.) * Correspondence: [email protected]; Tel.: +385-31-224-300 Received: 21 November 2019; Accepted: 18 December 2019; Published: 20 December 2019 Abstract: Slavonska šunka is a traditional dry-cured smoked ham. Smoking with open fire commonly results in the accumulation of polycyclic aromatic hydrocarbons (PAH) molecules in tissues. The objective of this research was to assess the types and concentrations of 16 PAHs in 30 samples of traditional dry-cured smoked ham Slavonska šunka. In general, all samples had high values of anthracene while higher levels of acenaphthylene were present in some samples. In sample SS1, the maximal value for anthracene reached 228.03 µg/kg, while sample SS28 had the lowest value of this PAH—33.38 µg/kg. SS1 had increased values for several other PAHs: benzo[a]anthracene (54.03 µg/kg), acenaphtene (19.90 µg/kg) and phenanthrene (27.11 µg/kg). Cancerogenic benzo[a]pyrene content was below the limit of quantification for all samples. -
Bakalářská Práce
MENDELOVA UNIVERZITA V BRNĚ AGRONOMICKÁ FAKULTA BAKALÁŘSKÁ PRÁCE BRNO 2017 NATÁLIE KOMÁRKOVÁ Mendelova univerzita v Brně Agronomická fakulta Ústav Technologie potravin Speciální masné výrobky Bakalářská práce Vedoucí práce: Vypracovala: prof. Ing. Alžbeta Jarošová, Ph.D. Natálie Komárková Brno 2017 Zadání bakalářské práce Prohlášení Prohlašuji, že jsem práci Speciální masné výrobky vypracovala samostatně a veškeré použité prameny a informace uvádím v seznamu použité literatury. Souhlasím, aby moje práce byla zveřejněna v souladu s § 47b zákona č. 111/1998 Sb., o vysokých školách a o změně a doplnění dalších zákonů (zákon o vysokých školách), ve znění pozdějších předpisů, a v souladu s platnou Směrnicí o zveřejňování vysokoškolských závěrečných prací. Jsem si vědoma, že se na moji práci vztahuje zákon č. 121/2000 Sb., autorský zákon, a že Mendelova univerzita v Brně má právo na uzavření licenční smlouvy a užití této práce jako školního díla podle § 60 odst. 1 autorského zákona. Dále se zavazuji, že před sepsáním licenční smlouvy o využití díla jinou osobou (subjektem) si vyžádám písemné stanovisko univerzity, že předmětná licenční smlouva není v rozporu s oprávněnými zájmy univerzity, a zavazuji se uhradit případný příspěvek na úhradu nákladů spojených se vznikem díla, a to až do jejich skutečné výše. V Brně dne ……………………………………. podpis Poděkování Ráda bych poděkovala vedoucí své bakalářské práce paní prof. Ing. Alžbetě Jarošové, Ph.D., za odborné vedení, vstřícný přístup, zapůjčenou literaturu, cenné rady a připomínky, které mi pomohly při vypracování této práce. Rovněž děkuji své rodině a kamarádům za podporu během celého studia. ABSTRAKT Bakalářská práce s názvem Speciální masné výrobky se věnuje základnímu pohledu na speciální masné výrobky. -
Effect of the Inclusion of Smoking Step in Iberian Sausage Processing on Levels of Polycyclic Aromatic Hydrocarbons Martín-Cabello L
1 Effect of the inclusion of smoking step in Iberian sausage processing on levels of polycyclic aromatic hydrocarbons Martín-Cabello L. 1, Carrapiso A.I. 1, Sánchez del Pulgar J. 2, Torrado C. 1, García C. 2 and Martín L. 1 1 Extremadura University, Animal Production and Food Science Dept., Agricultural Engineering School, Badajoz, Spain 2 Extremadura University, Animal Production and Food Science Dept., Faculty of Veterinary, Cáceres, Spain Abstract— A traditional Iberian pork sausage, pyrene, dibenz (a,h) anthracene, dibenzo (a,e) pyrene, salchichón, was elaborated including a treatment of cold dibenzo (a,h) pyrene, dibenzo (a,i) pyrene, dibenzo smoking for 18 hours using wood from Quercus ilex. The (a,l) pyrene, indeno (1,2,3-cd) pyrene, 5- levels of six polycyclic aromatic hydrocarbons (PAH) methylchrysene) were clearly recognized as (B(b)F, B(k)F, B(a)A, B(a)P, B(ghi)P and I(123-cd)P) carcinogenic, for which further investigation of the were analyzed at the end of processing. PAH were determined by HPLC-FLD. A control batch that was not relative levels was required by a Commission smoked was also evaluated. For the control group (n=15) Recommendation [7]. results showed that the six PAH analyzed in this study Benzo(a)pyrene (B[a]P) has been identified as very were not found in detectable amounts. In the smoked mutagenic and carcinogenic and it has been accepted batch (n=15) a total sum of the six PAH of 0.4µg/kg were as a general indicator of total PAH present in wood found, being low quantity probably due to the shorter smoke, smoked foods and environmental samples [8], period of smoking than those usually referred in other although it accounts between 1 and 20% of total PAH studies. -
Of Council Regulation (EEC) No 2081/92 on the Protection of Geographical Indications and Designations of Origin
C 42/2EN Official Journal of the European Communities 8.2.2001 Publication of an application for registration pursuant to Article 6(2) of Council Regulation (EEC) No 2081/92 on the protection of geographical indications and designations of origin (2001/C 42/02) This publication confers the right to object to the application pursuant to Article 7 of the abovementioned Regulation. Any objection to this application must be submitted via the competent authority in the Member State concerned within a time limit of six months from the date of this publication. The arguments for publication are set out below, in particular under 4.6, and are considered to justify the application within the meaning of Regulation (EEC) No 2081/92. COUNCIL REGULATION (EEC) No 2081/92 APPLICATION FOR REGISTRATION: ARTICLE 5 PDO ( ) PGI (x) National application No 64 1. Responsible department in the Member State Name: Subdirección General de Denominaciones de Calidad. Dirección General de Alimentación. Secretaría General de Agricultura y Ganadería. Ministerio de Agricultura, Pesca y Alimen- tación, Espaæa Address: Paseo de La Infanta Isabel, no 1, E-28071 Madrid Tel. (34) 913 47 53 94 Fax (34) 913 47 54 10 2. Applicant group 2.1. Name: Asociación Promotora Denominación Específica Botillo del Bierzo 2.2. Address: Avda Villafranca, 31, Bembibre, LØon Tel. (34) 987 45 60 96 Fax (34) 987 45 61 97 2.3. Composition: Producer/processor (x) other ( ) 3. Type of product: Class 1-2 Meat-based product. 4. Specification (summary of requirements under Article 4(2)) 4.1. Name: Botillo del Bierzo. 4.2. -
Physicochemical, Hygienic and Organoleptic Characterisation of Slavonian Kulen, a Traditional Pork Sausage from Eastern Croatia
Physicochemical, hygienic and organoleptic characterisation of Slavonian Kulen, a traditional pork sausage from eastern Croatia Karolyi D., Salajpal K., Luković Z., Kovačić D. in De Pedro E.J. (ed.), Cabezas A.B. (ed.). 7th International Symposium on the Mediterranean Pig Zaragoza : CIHEAM Options Méditerranéennes : Série A. Séminaires Méditerranéens; n. 101 2012 pages 281-288 Article available on line / Article disponible en ligne à l’adresse : -------------------------------------------------------------------------------------------------------------------------------------------------------------------------- http://om.ciheam.org/article.php?IDPDF=00006694 -------------------------------------------------------------------------------------------------------------------------------------------------------------------------- To cite this article / Pour citer cet article -------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Karolyi D., Salajpal K., Luković Z., Kovačić D. Physicochemical, hygienic and organoleptic characterisation of Slavonian Kulen, a traditional pork sausage from eastern Croatia. In : De Pedro E.J. (ed.), Cabezas A.B. (ed.). 7th International Symposium on the Mediterranean Pig. Zaragoza : CIHEAM, 2012. p. 281-288 (Options Méditerranéennes : Série A. Séminaires Méditerranéens; n. 101) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------- -
Assessment of the Overall Impact of Diet on Colorectal Cancer in Cambodia, with Special Emphasis on Endogenous and Exogenous Chemicals Sokneang In
Assessment of the overall impact of diet on colorectal cancer in Cambodia, with special emphasis on endogenous and exogenous chemicals Sokneang In To cite this version: Sokneang In. Assessment of the overall impact of diet on colorectal cancer in Cambodia, with special emphasis on endogenous and exogenous chemicals. Food engineering. AgroParisTech, 2012. English. NNT : 2012AGPT0014. pastel-01058622 HAL Id: pastel-01058622 https://pastel.archives-ouvertes.fr/pastel-01058622 Submitted on 27 Aug 2014 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Doctorat ParisTech T H È S E pour obtenir le grade de docteur délivré par L’Institut des Sciences et Industries du Vivant et de l’Environnement (AgroParisTech) Spécialité : Science and processes of Food and bio-products présentée et soutenue publiquement par Sokneang IN Soutenue le 17 février 2012 Evaluation de l’impact global des régimes alimentaires et des composés chimiques endogènes et exogènes sur le cancer colorectal au Cambodge Assessment of the overall impact of diet on colorectal cancer in Cambodia, with special emphasis on endogenous and exogenous chemicals Directeur de thèse : Claude LAMBRE Co-encadrants de la thèse : Mostafa OULD ELHKIM, Philippe VERGER Jury Mme Valérie CAMEL, Professeur, UMR GENIAL, AgroParisTech Présidente M. -
Iceland on the Protection of Geographical Indications for Agricultural Products and Foodstuffs
24.10.2017 EN Official Journal of the European Union L 274/3 AGREEMENT between the European Union and Iceland on the protection of geographical indications for agricultural products and foodstuffs THE EUROPEAN UNION, of the one part, and ICELAND of the other part, hereinafter referred to as the ‘Parties’, CONSIDERING that the Parties agree to promote between each other a harmonious development of the geographical indications as defined in Article 22(1) of the Agreement on Trade-Related Aspects of Intellectual Property Rights (TRIPS) and to foster trade in agricultural products and foodstuffs originating in the Parties' territories, CONSIDERING that the Agreement on the European Economic Area (EEA Agreement) provides for the mutual recognition and protection of geographical indications of wines, aromatised wine products and spirit drinks, HAVE AGREED AS FOLLOWS: Article 1 Scope 1. This Agreement applies to the recognition and protection of geographical indications for agricultural products and foodstuffs other than wines, aromatised wine products and spirit drinks originating in the Parties' territories. 2. Geographical indications of a Party shall be protected by the other Party under this Agreement only if covered by the scope of the legislation referred to in Article 2. Article 2 Established geographical indications 1. Having examined the legislation of Iceland listed in Part A of Annex I, the European Union concludes that that legislation meets the elements laid down in Part B of Annex I. 2. Having examined the legislation of the European Union listed in Part A of Annex I, Iceland concludes that that legislation meets the elements laid down in Part B of Annex I. -
Hercegovacka Pecenica
foods Article The Influence of Different Smoking Procedures on the Content of 16 PAHs in Traditional Dry Cured Smoked Meat “HercegovaˇckaPeˇcenica” Leona Pulji´c 1, Krešimir Mastanjevi´c 2,* , Brankica Kartalovi´c 3, Dragan Kovaˇcevi´c 2, Jelena Vraneševi´c 3 and Kristina Mastanjevi´c 2 1 The Faculty of Agriculture and Food Technology (APTF) of the University of Mostar, Biskupa Culeˇ bb, 88000 Mostar, Bosnia and Herzegovina; [email protected] 2 Faculty of Food Technology Osijek, Josip Juraj Strossmayer University of Osijek, F. Kuhaˇca20, 31000 Osijek, Croatia; [email protected] (D.K.); [email protected] (K.M.) 3 Scientific Veterinary Institute Novi Sad, Rumenaˇckiput 20, 21000 Novi Sad, Serbia; [email protected] (B.K.); [email protected] (J.V.) * Correspondence: [email protected]; Tel.: +385-31-224-300 Received: 15 November 2019; Accepted: 11 December 2019; Published: 17 December 2019 Abstract: During smoking, meat products may get contaminated by polycyclic aromatic hydrocarbons (PAH), especially the ones that are smoked in traditional (uncontrolled) conditions. This study aims to evaluate the difference in PAH content in samples of traditional dry cured pork meat products, “Hercegovaˇckapeˇcenica”,produced in (1) a traditional smokehouse and (2) in industrial chambers. The study revealed that the content of the four priority PAHs (PAH4) in samples produced in a traditional smoking manner highly exceeded (up to 10 times) the maximal limits set for PAHs (12 µg/kg). PAH4 in all samples subjected to industrial smoking procedures was below the limit of quantification. All samples had below-the-limit-of-quantification values for Benzo[a]pyrene. -
Influence of Traditional Smoking on Content Polycyclic Aromatic Hydrocarbons in Dry Fermented Sausages with Collagen and Natural Casings
INFLUENCE OF TRADITIONAL SMOKING ON CONTENT POLYCYCLIC AROMATIC HYDROCARBONS IN DRY FERMENTED SAUSAGES WITH COLLAGEN AND NATURAL CASINGS Škaljac Snežana1, Petrović Ljiljana1, Tasić Tatjana2, Ikonić Predrag2, Jokanović Marija1, Tomović Vladimir1, Džinić Natalija1, Šojić Branislav1, Tjapkin Ana1 and Škrbić Biljana1 1 Faculty of Technology, University of Novi Sad, Novi Sad, Serbia 2 Institute for Food Technology, University of Novi Sad, Novi Sad, Serbia Abstract – The concentrations of 13 polycyclic antimicrobial constituents such as phenols. It is aromatic hydrocarbons from Environmental still widely used in Serbian traditional dry Protection Agency list (EPA PAHs) were examined fermented sausages production (Petrovská in two groups of fermented dry sausages (Petrovská klobása) to obtain peculiar sensorial klobása) smoked on traditional way. The sausage characteristics due to phenol derivatives, carbonyls, mixture from the first group of samples was stuffed in the natural casings (N), while the second was organic acids. Wood smoke contains a large stuffed in collagen casings (C). Analyses of PAHs number of polycyclic aromatic hydrocarbons were determined using gas chromatography with (PAH) and their alkylated derivatives [4]. They mass spectrometry. Content of pyrene, anthracene, may be regarded as potentially genotoxic and acenaphthylene, fluorene and phenanthrene was carcinogenic to humans [5] due to a metabolic significantly (P<0.05) lower in sausage with collagen activation in mammalian cell to dioepoxides casings (2.7; 12.8; 10.1; 11.5; 19.1, µg/kg causing errors in DNA replication and mutation, respectively) than in the sausage with natural which initiates the carcinogenic process [6, 7]. casings (8.3; 16.0; 34.9; 38.0; 39.9 µg/kg The United States Environmental Protection respectively). -
Staphylococcal Ecosystem of Kitoza, a Traditional Malagasy Meat Product
International Journal of Food Microbiology 246 (2017) 20–24 Contents lists available at ScienceDirect International Journal of Food Microbiology journal homepage: www.elsevier.com/locate/ijfoodmicro Staphylococcal ecosystem of kitoza, a traditional malagasy meat product Angela Ratsimba a,SabineLeroyb,JeanPaulChacornacb, Danielle Rakoto a,ElodieArnaudc,d, Victor Jeannoda a,RégineTalonb,⁎ a UT, Université d'Antananarivo, Faculté des Sciences, Madagascar b MIC, INRA, 63122 Saint-Genès-Champanelle, France c UMR QUALISUD, CIRAD, 7602 Stellenbosch, South Africa d UMR QUALISUD, CIRAD, 34398 Montpellier, France article info abstract Article history: Kitoza is a traditional meat product from Madagascar manufactured with strips of pork or beef. The process in- Received 30 August 2016 cludes a first step of salting and mixing with spices followed by sun-drying or smoking step. As salting and drying Received in revised form 31 January 2017 select coagulase-negative staphylococci (CNS), our aim was to identify the CNS species in kitoza with the objec- Accepted 1 February 2017 tive in the future of developing indigenous starters. Microbial analyses revealed that the only pathogenic bacte- Available online 02 February 2017 rium enumerated was Staphylococcus aureus, which was found in 54% of the samples. The level of Enterobacteriaceae revealed a rather good hygienic quality of these products. CNS were confirmed in all the sam- Keywords: fi Kitoza ples at high levels ranging from 5 to 7 log cfu/g. Identi cation of CNS species in a large collection of 829 isolates Beef revealed 9 identified species, 7 for beef and 8 for pork kitoza. There were significant difference in the distribution Pork of CNS species according to the type of meat and the process.