Polyclonal Antibody to HUWE1 / UREB1 (C-Term) - Aff - Purified

OriGene Technologies, Inc. OriGene Technologies GmbH

9620 Medical Center Drive, Ste 200 Schillerstr. 5 Rockville, MD 20850 32052 Herford UNITED STATES GERMANY Phone: +1-888-267-4436 Phone: +49-5221-34606-0 Fax: +1-301-340-8606 Fax: +49-5221-34606-11 [email protected] [email protected]

AP07305PU-N Polyclonal Antibody to HUWE1 / UREB1 (C-term) - Aff - Purified

Alternate names: ARF-BP1, ARF-binding protein 1, E3 ubiquitin-protein ligase HUWE1, HECT, HSPC272, HectH9, Homologous to E6AP carboxyl terminus homologous protein 9, KIAA0312, KIAA1578, LASU1, Large structure of UREB1, Mcl-1 ubiquitin ligase E3, Mule, UBA and WWE domain-containing protein 1, URE-B1, URE-binding protein 1, Upstream regulatory element-binding protein 1 Quantity: 50 µg Concentration: 1 mg/ml Background: ARFBP1 is a novel E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Regulates apoptosis by catalyzing the polyubiquitination and degradation of MCL1. Also ubiquitinates the p53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Binds to an upstream initiator-like sequence in the preprodynorphin gene. Regulates neural differentiation and proliferation by catalyzing the polyubiquitination and degradation of MYCN. May regulate abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation. Uniprot ID: Q7Z6Z7 NCBI: NP_113584.3 GeneID: 10075 Host / Isotype: Rabbit / IgG Immunogen: Synthetic peptide corresponding to a 19 amino acid peptide from near the carboxy terminus of ARF-BP1. Format: State: Liquid purified IgG fraction Purification: Immunoaffinity Chromatography Buffer System: PBS containing 0.09% Sodium Azide as preservative Applications: Immunohistochemistry on Paraffin Sections: 5 µg/ml. Western Blot: 2 µg/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: This antibody is specific to HUWE1/ARFBP1. Species: Human, Mouse and Rat. Other species not tested. Storage: Store undiluted at 2-8°C. Shelf life: one year from despatch. Product Citations: Originator or purchased from resellers: 1. Gruber S, Straub BK, Ackermann PJ, Wunderlich CM, Mauer J, Seeger JM, et al. Obesity promotes liver carcinogenesis via Mcl-1 stabilization independent of IL-6Rα signaling. Cell Rep. 2013 Aug 29;4(4):669-80. doi: 10.1016/j.celrep.2013.07.023. Epub 2013 Aug 15. PubMed PMID: 23954788.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20170919 1 / 2 AP07305PU-N: Polyclonal Antibody to HUWE1 / UREB1 (C-term) - Aff - Purified

Pictures: Heart: Formalin-Fixed Paraffin-Embedded (FFPE)

Brain, cortex: Formalin-Fixed Paraffin- Embedded (FFPE)

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20170919 2 / 2