Susan Weiss Slides

Total Page：16

File Type：pdf, Size：1020Kb

Susan R. Weiss Department of Microbiology Penn Center for Research on Coronaviruses and Other Emerging Pathogens Perelman School of Medicine University of Pennsylvania Coronavirus virion Spike (S) Nucleocapsid (N) entry: binding & fusion CAP tropism immune Membrane (M) response virulence Small membrane Protein (E) AA AA single‐stranded, Coronaviruses are a family within the Nidovirus non‐segmented, order; named for the nested subgenomic mRNAs positive sense RNA Hemagglutinin‐esterase (HE) genome generated during infection Coronavirus Timeline Bat SARS‐CoV CoVs OC43 Basic CoV Biology MERS‐CoV SARS‐CoV‐2 229E HKU1 Common cold NL63 OC43 can infect HKU1; Pneumonia lower respiratory NL63; Bronchiolitis, croup tract 1960‐70 1980 1990 2000 2010 2020 MHV model Animal viruses, vaccines Pathogenic human coronaviruses Human cold CoVs SARS‐CoV, MERS‐CoV, SARS‐CoV‐2 Severe acute respiratory disease coronavirus disease‐2019 or COVID‐19 SARS‐CoV interspecies transmission Human Horseshoe bat Civet Human to human close contact How many times did this happen? Over in eight months, 8098 infections, 9.6% mortality 87% in China and Hong Kong Images from various internet sites MERS‐CoV interspecies transmission limited human Human to human spread Neoromicia capensis Camel Bat Mostly in Arabian peninsula Camels are a reservoir for MERS‐CoV Still new cases 2020 2428 cases, 35% mortality Korea SARS‐CoV‐2 interspecies transmission ???? Intermediate species Bat Malayan pangolin Human Human to human spread 34.7 M infections 1,030,880 deaths US (10.02.20) 7.3 infections 209,318 deaths Coronavirus genome structure Conserved replicase proteins 16 nsps Accessory proteins Alphacoronaviruses 1a 1b 4a 5’ PLP1 PLP2 3CLpro 4b Duvinacovirus Pol Hel AAA3’ 229E, NL63 Setracovirus SPIKE EM N Betacoronaviruses 1a 5a 1b 2a 4 Lineage a, 5’ AAA3’ OC43, HKU1, MHV Embecovirus HE SPIKE EM IN 7a/7b8a/8b Lineage b, 1a 3a/3b 6 1b 5 9b Sarbecovirus 5’ AAA3’ SARS‐CoV SPIKE E SARS‐CoV‐2 M N 1a 4a 5 Lineage c, 1b 5’ Merbecovirus AAA3’ MERS‐CoV SPIKE 3 4b EM8b N Coronavirus conserved proteases and replicase ORF1a ORF 1b nsp 12 3 45678910 11 12 13 1415 16 nsp10 Papain‐like 3C‐like nsp8 activator nsp1 nsp16 protease(s) protease primase nsp13 nsp15 mulitple host RNA dependent mRNA processes helicase EndoU; antagonist polyprotein RNA polymerase dsRNA capping activities main protease RdRp NTPase nsp3 accumulation processes Replication of genome host antagonist polyprotein and transcription of activities nsp14 mRNAs DUB ExoN macrodomain remdesivir Proof reading mRNA capping How do we know SARS‐CoV‐2 was not engineered by humans? • Similar viruses are found in bats • It does not resemble any known recombinant viruses • It seems impossible that anyone would know how to design a virus with the properties of SARS‐CoV‐2 For the future • Vaccine development • Develop antivirals for future outbreaks or spillovers • Spike protein RBD may differ while replicase proteins are conserved • Continue to identify and characterize Coronaviruses and other viruses from bats and other species • Support basic virology research.

Copy Link

Recommended publications

Identification of a Novel Betacoronavirus (Merbecovirus) in Amur Hedgehogs from China
viruses Article Identiﬁcation of a Novel Betacoronavirus (Merbecovirus) in Amur Hedgehogs from China 1,2,3,4, 1, 1, 1 Susanna K. P. Lau y, Hayes K. H. Luk y , Antonio C. P. Wong y, Rachel Y. Y. Fan , Carol S. F. Lam 1, Kenneth S. M. Li 1, Syed Shakeel Ahmed 1, Franklin W.N. Chow 1 , Jian-Piao Cai 1, Xun Zhu 5,6, Jasper F. W. Chan 1,2,3,4 , Terrence C. K. Lau 7 , Kaiyuan Cao 5,6, Mengfeng Li 5,6, Patrick C. Y. Woo 1,2,3,4,* and Kwok-Yung Yuen 1,2,3,4,* 1 Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong 999077, China; [email protected] (S.K.P.L.); [email protected] (H.K.H.L.); [email protected] (A.C.P.W.); [email protected] (R.Y.Y.F.); [email protected] (C.S.F.L.); [email protected] (K.S.M.L.); [email protected] (S.S.A.); [email protected] (F.W.N.C.); [email protected] (J.-P.C.); [email protected] (J.F.W.C.) 2 State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong 999077, China 3 Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong 999077, China 4 Collaborative Innovation Centre for Diagnosis and Treatment of Infectious Diseases, The University of Hong Kong, Hong Kong 999077, China 5 Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China; [email protected] (X.Z.); [email protected] (K.C.); [email protected] (M.L.) 6 Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of Education, Guangzhou 510080, China 7 Department of Biomedical Sciences, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Hong Kong 999077, China; [email protected] * Correspondence: [email protected] (P.C.Y.W.); [email protected] (K.-Y.Y.); Tel.: +852-2255-4892 (P.C.Y.W.
[Show full text]
Exposure of Humans Or Animals to Sars-Cov-2 from Wild, Livestock, Companion and Aquatic Animals Qualitative Exposure Assessment
ISSN 0254-6019 Exposure of humans or animals to SARS-CoV-2 from wild, livestock, companion and aquatic animals Qualitative exposure assessment FAO ANIMAL PRODUCTION AND HEALTH / PAPER 181 FAO ANIMAL PRODUCTION AND HEALTH / PAPER 181 Exposure of humans or animals to SARS-CoV-2 from wild, livestock, companion and aquatic animals Qualitative exposure assessment Authors Ihab El Masry, Sophie von Dobschuetz, Ludovic Plee, Fairouz Larfaoui, Zhen Yang, Junxia Song, Wantanee Kalpravidh, Keith Sumption Food and Agriculture Organization for the United Nations (FAO), Rome, Italy Dirk Pfeiffer City University of Hong Kong, Hong Kong SAR, China Sharon Calvin Canadian Food Inspection Agency (CFIA), Science Branch, Animal Health Risk Assessment Unit, Ottawa, Canada Helen Roberts Department for Environment, Food and Rural Affairs (Defra), Equines, Pets and New and Emerging Diseases, Exotic Disease Control Team, London, United Kingdom of Great Britain and Northern Ireland Alessio Lorusso Istituto Zooprofilattico dell’Abruzzo e Molise, Teramo, Italy Casey Barton-Behravesh Centers for Disease Control and Prevention (CDC), One Health Office, National Center for Emerging and Zoonotic Infectious Diseases, Atlanta, United States of America Zengren Zheng China Animal Health and Epidemiology Centre (CAHEC), China Animal Health Risk Analysis Commission, Qingdao City, China Food and Agriculture Organization of the United Nations Rome, 2020 Required citation: El Masry, I., von Dobschuetz, S., Plee, L., Larfaoui, F., Yang, Z., Song, J., Pfeiffer, D., Calvin, S., Roberts, H., Lorusso, A., Barton-Behravesh, C., Zheng, Z., Kalpravidh, W. & Sumption, K. 2020. Exposure of humans or animals to SARS-CoV-2 from wild, livestock, companion and aquatic animals: Qualitative exposure assessment. FAO animal production and health, Paper 181.
[Show full text]
On the Coronaviruses and Their Associations with the Aquatic Environment and Wastewater
water Review On the Coronaviruses and Their Associations with the Aquatic Environment and Wastewater Adrian Wartecki 1 and Piotr Rzymski 2,* 1 Faculty of Medicine, Poznan University of Medical Sciences, 60-812 Poznań,Poland; [email protected] 2 Department of Environmental Medicine, Poznan University of Medical Sciences, 60-806 Poznań,Poland * Correspondence: [email protected] Received: 24 April 2020; Accepted: 2 June 2020; Published: 4 June 2020 Abstract: The outbreak of Coronavirus Disease 2019 (COVID-19), a severe respiratory disease caused by betacoronavirus SARS-CoV-2, in 2019 that further developed into a pandemic has received an unprecedented response from the scientific community and sparked a general research interest into the biology and ecology of Coronaviridae, a family of positive-sense single-stranded RNA viruses. Aquatic environments, lakes, rivers and ponds, are important habitats for bats and birds, which are hosts for various coronavirus species and strains and which shed viral particles in their feces. It is therefore of high interest to fully explore the role that aquatic environments may play in coronavirus spread, including cross-species transmissions. Besides the respiratory tract, coronaviruses pathogenic to humans can also infect the digestive system and be subsequently defecated. Considering this, it is pivotal to understand whether wastewater can play a role in their dissemination, particularly in areas with poor sanitation. This review provides an overview of the taxonomy, molecular biology, natural reservoirs and pathogenicity of coronaviruses; outlines their potential to survive in aquatic environments and wastewater; and demonstrates their association with aquatic biota, mainly waterfowl. It also calls for further, interdisciplinary research in the field of aquatic virology to explore the potential hotspots of coronaviruses in the aquatic environment and the routes through which they may enter it.
[Show full text]
Broad Sarbecovirus Neutralization by a Human Monoclonal Antibody
Article Broad sarbecovirus neutralization by a human monoclonal antibody https://doi.org/10.1038/s41586-021-03817-4 M. Alejandra Tortorici1,2,9, Nadine Czudnochowski3,9, Tyler N. Starr4,9, Roberta Marzi5,9, Alexandra C. Walls1, Fabrizia Zatta5, John E. Bowen1, Stefano Jaconi5, Julia Di Iulio3, Received: 29 March 2021 Zhaoqian Wang1, Anna De Marco5, Samantha K. Zepeda1, Dora Pinto5, Zhuoming Liu6, Accepted: 9 July 2021 Martina Beltramello5, Istvan Bartha5, Michael P. Housley3, Florian A. Lempp3, Laura E. Rosen3, Exequiel Dellota Jr3, Hannah Kaiser3, Martin Montiel-Ruiz3, Jiayi Zhou3, Amin Addetia4, Published online: 19 July 2021 Barbara Guarino3, Katja Culap5, Nicole Sprugasci5, Christian Saliba5, Eneida Vetti5, Check for updates Isabella Giacchetto-Sasselli5, Chiara Silacci Fregni5, Rana Abdelnabi7, Shi-Yan Caroline Foo7, Colin Havenar-Daughton3, Michael A. Schmid5, Fabio Benigni5, Elisabetta Cameroni5, Johan Neyts7, Amalio Telenti3, Herbert W. Virgin3, Sean P. J. Whelan6, Gyorgy Snell3, Jesse D. Bloom4,8, Davide Corti5 ✉, David Veesler1 ✉ & Matteo Samuele Pizzuto5 ✉ The recent emergence of SARS-CoV-2 variants of concern1–10 and the recurrent spillovers of coronaviruses11,12 into the human population highlight the need for broadly neutralizing antibodies that are not afected by the ongoing antigenic drift and that can prevent or treat future zoonotic infections. Here we describe a human monoclonal antibody designated S2X259, which recognizes a highly conserved cryptic epitope of the receptor-binding domain and cross-reacts with spikes from all clades of sarbecovirus. S2X259 broadly neutralizes spike-mediated cell entry of SARS-CoV-2, including variants of concern (B.1.1.7, B.1.351, P.1, and B.1.427/B.1.429), as well as a wide spectrum of human and potentially zoonotic sarbecoviruses through inhibition of angiotensin-converting enzyme 2 (ACE2) binding to the receptor-binding domain.
[Show full text]
A New Coronavirus Associated with Human Respiratory Disease in China
Article A new coronavirus associated with human respiratory disease in China https://doi.org/10.1038/s41586-020-2008-3 Fan Wu1,7, Su Zhao2,7, Bin Yu3,7, Yan-Mei Chen1,7, Wen Wang4,7, Zhi-Gang Song1,7, Yi Hu2,7, Zhao-Wu Tao2, Jun-Hua Tian3, Yuan-Yuan Pei1, Ming-Li Yuan2, Yu-Ling Zhang1, Fa-Hui Dai1, Received: 7 January 2020 Yi Liu1, Qi-Min Wang1, Jiao-Jiao Zheng1, Lin Xu1, Edward C. Holmes1,5 & Yong-Zhen Zhang1,4,6 ✉ Accepted: 28 January 2020 Published online: 3 February 2020 Emerging infectious diseases, such as severe acute respiratory syndrome (SARS) and Open access Zika virus disease, present a major threat to public health1–3. Despite intense research Check for updates eforts, how, when and where new diseases appear are still a source of considerable uncertainty. A severe respiratory disease was recently reported in Wuhan, Hubei province, China. As of 25 January 2020, at least 1,975 cases had been reported since the frst patient was hospitalized on 12 December 2019. Epidemiological investigations have suggested that the outbreak was associated with a seafood market in Wuhan. Here we study a single patient who was a worker at the market and who was admitted to the Central Hospital of Wuhan on 26 December 2019 while experiencing a severe respiratory syndrome that included fever, dizziness and a cough. Metagenomic RNA sequencing4 of a sample of bronchoalveolar lavage fuid from the patient identifed a new RNA virus strain from the family Coronaviridae, which is designated here ‘WH-Human 1’ coronavirus (and has also been referred to as ‘2019-nCoV’).
[Show full text]
Drawing Comparisons Between SARS-Cov-2 and the Animal Coronaviruses
microorganisms Review Drawing Comparisons between SARS-CoV-2 and the Animal Coronaviruses Souvik Ghosh 1,* and Yashpal S. Malik 2 1 Department of Biomedical Sciences, Ross University School of Veterinary Medicine, Basseterre 334, Saint Kitts and Nevis 2 College of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Science University, Ludhiana 141004, India; [email protected] * Correspondence: [email protected] or [email protected]; Tel.: +1-869-4654161 (ext. 401-1202) Received: 23 September 2020; Accepted: 19 November 2020; Published: 23 November 2020 Abstract: The COVID-19 pandemic, caused by a novel zoonotic coronavirus (CoV), SARS-CoV-2, has infected 46,182 million people, resulting in 1,197,026 deaths (as of 1 November 2020), with devastating and far-reaching impacts on economies and societies worldwide. The complex origin, extended human-to-human transmission, pathogenesis, host immune responses, and various clinical presentations of SARS-CoV-2 have presented serious challenges in understanding and combating the pandemic situation. Human CoVs gained attention only after the SARS-CoV outbreak of 2002–2003. On the other hand, animal CoVs have been studied extensively for many decades, providing a plethora of important information on their genetic diversity, transmission, tissue tropism and pathology, host immunity, and therapeutic and prophylactic strategies, some of which have striking resemblance to those seen with SARS-CoV-2. Moreover, the evolution of human CoVs, including SARS-CoV-2, is intermingled with those of animal CoVs. In this comprehensive review, attempts have been made to compare the current knowledge on evolution, transmission, pathogenesis, immunopathology, therapeutics, and prophylaxis of SARS-CoV-2 with those of various animal CoVs.
[Show full text]
Isolation of Cross-Reactive Monoclonal Antibodies Against Divergent Human
bioRxiv preprint doi: https://doi.org/10.1101/2020.10.20.346916; this version posted October 20, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Isolation of cross-reactive monoclonal antibodies against divergent human 2 coronaviruses that delineate a conserved and vulnerable site on the spike 3 protein 4 5 6 Chunyan Wanga, Rien van Haperenb,c, Javier Gutiérrez-Álvarezd, Wentao Lia, Nisreen 7 M.A. Okbae, Irina Albulescua, Ivy Widjajaa,‡, Brenda van Dierena,‡, Raul Fernandez- 8 Delgadod, Isabel Solad, Daniel L. Hurdissa, Olalekan Daramolaf, Frank Grosveldb,c, 9 Frank J.M. van Kuppevelda, Bart L. Haagmanse, Luis Enjuanesd, Dubravka Drabekb,c 10 and Berend-Jan Boscha,* 11 12 Division of Infectious Diseases and Immunology, Department of Biomolecular Health 13 Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlandsa; 14 Department of Cell Biology, Erasmus Medical Center, Rotterdam, the Netherlandsb; 15 Harbour BioMed, Rotterdam, the Netherlandsc; Department of Molecular and Cell 16 Biology, National Center for Biotechnology-Spanish National Research Council (CNB- 17 CSIC), Madrid, Spaind; Department of Viroscience, Erasmus Medical Center, 18 Rotterdam, the Netherlandse; Cell Culture and Fermentation Sciences, 19 Biopharmaceutical Development, BioPharmaceuticals R&D, AstraZeneca, Cambridge, 20 United Kingdomf. 21 22 ‡ Present address: Merus N.V., Utrecht, the Netherlands. 23 * Address correspondence to Berend-Jan Bosch ([email protected]) 1 bioRxiv preprint doi: https://doi.org/10.1101/2020.10.20.346916; this version posted October 20, 2020.
[Show full text]
Betacoronavirus Genomes: How Genomic Information Has Been Used to Deal with Past Outbreaks and the COVID-19 Pandemic
International Journal of Molecular Sciences Review Betacoronavirus Genomes: How Genomic Information Has Been Used to Deal with Past Outbreaks and the COVID-19 Pandemic Alejandro Llanes 1 , Carlos M. Restrepo 1 , Zuleima Caballero 1 , Sreekumari Rajeev 2 , Melissa A. Kennedy 3 and Ricardo Lleonart 1,* 1 Centro de Biología Celular y Molecular de Enfermedades, Instituto de Investigaciones Cientíﬁcas y Servicios de Alta Tecnología (INDICASAT AIP), Panama City 0801, Panama; [email protected] (A.L.); [email protected] (C.M.R.); [email protected] (Z.C.) 2 College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USA; [email protected] 3 College of Veterinary Medicine, University of Tennessee, Knoxville, TN 37996, USA; [email protected] * Correspondence: [email protected]; Tel.: +507-517-0740 Received: 29 May 2020; Accepted: 23 June 2020; Published: 26 June 2020 Abstract: In the 21st century, three highly pathogenic betacoronaviruses have emerged, with an alarming rate of human morbidity and case fatality. Genomic information has been widely used to understand the pathogenesis, animal origin and mode of transmission of coronaviruses in the aftermath of the 2002–2003 severe acute respiratory syndrome (SARS) and 2012 Middle East respiratory syndrome (MERS) outbreaks. Furthermore, genome sequencing and bioinformatic analysis have had an unprecedented relevance in the battle against the 2019–2020 coronavirus disease 2019 (COVID-19) pandemic, the newest and most devastating outbreak caused by a coronavirus in the history of mankind. Here, we review how genomic information has been used to tackle outbreaks caused by emerging, highly pathogenic, betacoronavirus strains, emphasizing on SARS-CoV, MERS-CoV and SARS-CoV-2.
[Show full text]
Characterization of Accessory Genes in Coronavirus Genomes Christian Jean Michel1, Claudine Mayer1,2,3, Olivier Poch1 and Julie Dawn Thompson1*
Michel et al. Virology Journal (2020) 17:131 https://doi.org/10.1186/s12985-020-01402-1 RESEARCH Open Access Characterization of accessory genes in coronavirus genomes Christian Jean Michel1, Claudine Mayer1,2,3, Olivier Poch1 and Julie Dawn Thompson1* Abstract Background: The Covid19 infection is caused by the SARS-CoV-2 virus, a novel member of the coronavirus (CoV) family. CoV genomes code for a ORF1a / ORF1ab polyprotein and four structural proteins widely studied as major drug targets. The genomes also contain a variable number of open reading frames (ORFs) coding for accessory proteins that are not essential for virus replication, but appear to have a role in pathogenesis. The accessory proteins have been less well characterized and are difficult to predict by classical bioinformatics methods. Methods: We propose a computational tool GOFIX to characterize potential ORFs in virus genomes. In particular, ORF coding potential is estimated by searching for enrichment in motifs of the X circular code, that is known to be over-represented in the reading frames of viral genes. Results: We applied GOFIX to study the SARS-CoV-2 and related genomes including SARS-CoV and SARS-like viruses from bat, civet and pangolin hosts, focusing on the accessory proteins. Our analysis provides evidence supporting the presence of overlapping ORFs 7b, 9b and 9c in all the genomes and thus helps to resolve some differences in current genome annotations. In contrast, we predict that ORF3b is not functional in all genomes. Novel putative ORFs were also predicted, including a truncated form of the ORF10 previously identified in SARS- CoV-2 and a little known ORF overlapping the Spike protein in Civet-CoV and SARS-CoV.
[Show full text]
Overview of Bat and Wildlife Coronavirus Surveillance in Africa: a Framework for Global Investigations
viruses Review Overview of Bat and Wildlife Coronavirus Surveillance in Africa: A Framework for Global Investigations Marike Geldenhuys 1 , Marinda Mortlock 1 , Jonathan H. Epstein 1,2 , Janusz T. Paw˛eska 1,3 , Jacqueline Weyer 1,3,4 and Wanda Markotter 1,* 1 Centre for Viral Zoonoses, Department of Medical Virology, Faculty of Health Sciences, University of Pretoria, Pretoria 0001, South Africa; [email protected] (M.G.); [email protected] (M.M.); [email protected] (J.H.E.); [email protected] (J.T.P.); [email protected] (J.W.) 2 EcoHealth Alliance, New York, NY 10001, USA 3 Centre for Emerging Zoonotic and Parasitic Diseases, National Institute for Communicable Diseases, Johannesburg 2131, South Africa 4 Department of Microbiology and Infectious Diseases, School of Pathology, University of Witwatersrand, Johannesburg 2131, South Africa * Correspondence: [email protected] Abstract: The ongoing coronavirus disease 2019 (COVID-19) pandemic has had devastating health and socio-economic impacts. Human activities, especially at the wildlife interphase, are at the core of forces driving the emergence of new viral agents. Global surveillance activities have identified bats as the natural hosts of diverse coronaviruses, with other domestic and wildlife animal species possibly acting as intermediate or spillover hosts. The African continent is confronted by several factors that challenge prevention and response to novel disease emergences, such as high species diversity, inadequate health systems, and drastic social and ecosystem changes. We reviewed published animal coronavirus surveillance studies conducted in Africa, specifically summarizing surveillance approaches, species numbers tested, and findings. Far more surveillance has been Citation: Geldenhuys, M.; Mortlock, initiated among bat populations than other wildlife and domestic animals, with nearly 26,000 bat M.; Epstein, J.H.; Paw˛eska,J.T.; Weyer, J.; Markotter, W.
[Show full text]
2019 Ncov/SARS‐Cov‐2: Rapid Classification of Betacoronaviruses
Letters in Applied Microbiology ISSN 0266-8254 ORIGINAL ARTICLE Editor 2019_nCoV/SARS-CoV-2: rapid classification of betacoronaviruses and identification of Traditional Chinese ’ Medicine as potential origin of zoonotic coronaviruses s Choice T.M. Wassenaar1 and Y. Zou2 1 Molecular Microbiology and Genomics Consultants, Zotzenheim, Germany 2 SciPaperEdit, Chuangkexing, Wuhan, China Significance and Impact of the Study: The noncoding upstream and downstream flanks of coronavirus genomes allow for rapid classification of novel Betacoronavirus species and correct identification of genetic relationships. Although bats are the likely natural host of 2019_nCoV, the exact bat species that serves as the natural host of the virus remains as yet unknown. Chinese bat species with commercial value were identified as natural reservoirs of coronaviruses and are used in Traditional Chinese Medi- cine. Since their trading provides a potential risk for spreading zoonoses, a change in these practices is highly recommended. Keywords Abstract bats, coronavirus, epidemic, Sarbecovirus, Traditional Chinese Medicine, whole-genome The current outbreak of a novel severe acute respiratory syndrome-like comparison, zoonosis. coronavirus, 2019_nCoV (now named SARS-CoV-2), illustrated difficulties in identifying a novel coronavirus and its natural host, as the coding sequences of Correspondence various Betacoronavirus species can be highly diverse. By means of whole- Trudy M. Wassenaar, Molecular Microbiology genome sequence comparisons, we demonstrate that the noncoding flanks of and Genomics Consultants, Tannenstrasse 7, the viral genome can be used to correctly separate the recognized four 55576 Zotzenheim, Germany. E-mail: [email protected] betacoronavirus subspecies. The conservation would be sufficient to define target sequences that could, in theory, classify novel virus species into their 2020/0190: received 5 February 2020, revised subspecies.
[Show full text]
Animal Coronaviruses in the Light of COVID-19
J Vet Res 64, 333-345, 2020 DOI:10.2478/jvetres-2020-0050 REVIEW ARTICLE Animal coronaviruses in the light of COVID-19 Katarzyna Domańska-Blicharz1, Grzegorz Woźniakowski2, Bogdan Konopka3, Krzysztof Niemczuk4, Mirosław Welz3, Jerzy Rola5, Wojciech Socha5, Anna Orłowska5, Marta Antas2, Krzysztof Śmietanka1, Beata Cuvelier-Mizak6 1Department of Poultry Diseases, 2Department of Swine Diseases, 4Director General, 5Department of Virology, 6Department of Veterinary Pharmacy, National Veterinary Research Institute, 24-100 Puławy, Poland 3General Veterinary Inspectorate, 00-930 Warsaw, Poland [email protected] Received: July 1, 2020 Accepted: July 20, 2020 Abstract Coronaviruses are extremely susceptible to genetic changes due to the characteristic features of the genome structure, life cycle and environmental pressure. Their remarkable variability means that they can infect many different species of animals and cause different disease symptoms. Moreover, in some situations, coronaviruses might be transmitted across species. Although they are commonly found in farm, companion and wild animals, causing clinical and sometimes serious signs resulting in significant economic losses, not all of them have been classified by the World Organization for Animal Health (OIE) as hazardous and included on the list of notifiable diseases. Currently, only three diseases caused by coronaviruses are on the OIE list of notifiable terrestrial and aquatic animal diseases. However, none of these three entails any administrative measures. The emergence of the SARS-CoV-2 infections that have caused the COVID-19 pandemic in humans has proved that the occurrence and variability of coronaviruses is highly underestimated in the animal reservoir and reminded us of the critical importance of the One Health approach.
[Show full text]