SANTA CRUZ BIOTECHNOLOGY, INC. EML1 (3E8): sc-100938

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BACKGROUND SOURCE Echinoderm microtubule-associated function to modify the assembly EML1 (3E8) is a mouse monoclonal antibody raised against recombinant dynamics of microtubules by making microtubules slightly longer yet more EML1 of human origin. dynamic. They are classified as ubiquitous due to their expression in most tis- sues, however, their expression does not occur in the thymus nor the peripher- PRODUCT al blood lymphocytes. In the human form of the , there is a WD40 Each vial contains 100 µl ascites containing IgM with < 0.1% sodium azide. domain, also contained in a number of eukaryotic proteins, that carries out functions including signal transduction using adaptor/regulatory modules, APPLICATIONS pre-mRNA processing and cytoskeleton assembly. EML1 (echinoderm micro- tubule-associated protein-like 1 or EMAP-1) may be a candidate for EML1 (3E8) is recommended for detection of EML1 of human origin by type 1A. Usher syndromes (USHs) are a group of genetic dis- Western Blotting (starting dilution to be determined by researcher, dilution orders consisting of congenital deafness, , and vestibular range 1:100-1:5000), immunoprecipitation [1-2 µl per 100-500 µg of total dysfunction of variable onset and severity depending on the genetic type. protein (1 ml of cell lysate)] and solid phase ELISA (starting dilution to be determined by researcher, dilution range 1:100-1:5000). REFERENCES Suitable for use as control antibody for EML1 siRNA (h): sc-60576, EML1 1. Li, Q. and Suprenant, K.A. 1995. Molecular characterization of the 77-kDa shRNA Plasmid (h): sc-60576-SH and EML1 shRNA (h) Lentiviral Particles: echinoderm microtubule-associated protein. Homology to the β-transducin sc-60576-V. family. J. Biol. Chem. 269: 31777-31784. Molecular Weight of EML1: 79 kDa. 2. Eudy, J.D., Ma-Edmonds, M., Yao, S.F., Talmadge, C.B., Kelley, P.M., Weston, Positive Controls: Hep G2 cell lysate: sc-2227. M.D., Kimberling, W.J. and Sumegi, J. 1997. Isolation of a novel human homologue of the gene coding for echinoderm microtubule-associated pro- RECOMMENDED SECONDARY REAGENTS tein (EMAP) from the Usher syndrome type 1a locus at 14q32. Genomics 43: To ensure optimal results, the following support (secondary) reagents are 104-106. recommended: 1) Western Blotting: use goat anti-mouse IgM-HRP: sc-2064 3. Daggett, M.A., Li, Q., Weaver, R.F. and Suprenant, K.A. 1998. Overexpression (dilution range: 1:500-1:5,000), TBS Blotto A Blocking Reagent: sc-2333 and of the 77-kD echinoderm microtubule-associated protein (EMAP), a WD-40 Western Blotting Luminol Reagent: sc-2048. 2) Immunoprecipitation: use repeat protein, in baculovirus-infected Sf9 cells. Cell Motil. Cytoskeleton 41: Protein L PLUS-Agarose: sc-2336 (0.5 ml agarose/2.0 ml). 57-67. 4. Suprenant, K.A., Tuxhorn, J.A., Daggett, M.A., Ahrens, D.P., Hostetler, A., DATA Palange, J.M., VanWinkle, C.E. and Livingston, B.T. 2000. Conservation of the WD-repeat, microtubule-binding protein, EMAP, in sea urchins, humans, and the nematode C. elegans. Dev. Evol. 210: 2-10. 250 K – 150 K – 5. Eichenmüller, B., Ahrens, D.P., Li, Q. and Suprenant, K.A. 2002. Saturable 100 K – < EML1 binding of the echinoderm microtubule-associated protein (EMAP) on micro- 75 K – tubules, but not filamentous Actin or Vimentin filaments. Cell Motil. Cyto- skeleton 50: 161-172. 50 K – 6. De Keersmaecker, K., Graux, C., Odero, M.D., Mentens, N., Somers, R., EML1 (3E8): sc-100938. Western blot analysis of EML1 Maertens, J., Wlodarska, I., Vandenberghe, P., Hagemeijer, A., Marynen, P. expression in HepG2 whole cell lysate. and Cools, J. 2005. Fusion of EML1 to ABL1 in T-cell acute lymphoblastic leukemia with cryptic t(9;14)(q34;q32). Blood 105: 4849-4852. RESEARCH USE 7. http://harvester.embl.de/harvester/O004/O00423.htm For research use only, not for use in diagnostic procedures.

CHROMOSOMAL LOCATION PROTOCOLS Genetic locus: EML1 (human) mapping to 14q32.2. See our web site at www.scbt.com or our catalog for detailed protocols and support products. STORAGE For immediate and continuous use, store at 4° C for up to one month. For sporadic use, freeze in working aliquots in order to avoid repeated freeze/ thaw cycles. If turbidity is evident upon prolonged storage, clarify solution by centrifugation.

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