Specimen Label Users Should: • Remove Clothing/PPE Immediately If Pesticide Gets Inside
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They Come in Teams
GBE Frankia-Enriched Metagenomes from the Earliest Diverging Symbiotic Frankia Cluster: They Come in Teams Thanh Van Nguyen1, Daniel Wibberg2, Theoden Vigil-Stenman1,FedeBerckx1, Kai Battenberg3, Kirill N. Demchenko4,5, Jochen Blom6, Maria P. Fernandez7, Takashi Yamanaka8, Alison M. Berry3, Jo¨ rn Kalinowski2, Andreas Brachmann9, and Katharina Pawlowski 1,* 1Department of Ecology, Environment and Plant Sciences, Stockholm University, Sweden 2Center for Biotechnology (CeBiTec), Bielefeld University, Germany 3Department of Plant Sciences, University of California, Davis 4Laboratory of Cellular and Molecular Mechanisms of Plant Development, Komarov Botanical Institute, Russian Academy of Sciences, Saint Petersburg, Russia 5Laboratory of Molecular and Cellular Biology, All-Russia Research Institute for Agricultural Microbiology, Saint Petersburg, Russia 6Bioinformatics and Systems Biology, Justus Liebig University, Gießen, Germany 7Ecologie Microbienne, Centre National de la Recherche Scientifique UMR 5557, Universite Lyon I, Villeurbanne Cedex, France 8Forest and Forestry Products Research Institute, Ibaraki, Japan 9Biocenter, Ludwig Maximilians University Munich, Planegg-Martinsried, Germany *Corresponding author: E-mail: [email protected]. Accepted: July 10, 2019 Data deposition: This project has been deposited at EMBL/GenBank/DDBJ under the accession PRJEB19438 - PRJEB19449. Abstract Frankia strains induce the formation of nitrogen-fixing nodules on roots of actinorhizal plants. Phylogenetically, Frankia strains can be grouped in four clusters. The earliest divergent cluster, cluster-2, has a particularly wide host range. The analysis of cluster-2 strains has been hampered by the fact that with two exceptions, they could never be cultured. In this study, 12 Frankia-enriched meta- genomes of Frankia cluster-2 strains or strain assemblages were sequenced based on seven inoculum sources. Sequences obtained via DNA isolated from whole nodules were compared with those of DNA isolated from fractionated preparations enhanced in the Frankia symbiotic structures. -
Forest Service Research Natural Areas
1.Adorni 1.Adorni (Cheng 1997a, Sawyer 1981a) Location This established RNA is on the Six Rivers National Forest. It lies about 3 miles (5 km) N. of Weitchpec, Humboldt County, covering portions of sects. 25 and 26 T10N, R4E HBM (41°14'N., 123°41'W.), USGS Weitchpec quad (fig. 3). Ecological subsections – Gasquet Mountain Ultramafics (M261Ab) and Eastern Franciscan (M261Ba). Target Element Port Orford-Cedar (Chamaecyparis lawsoniana) Distinctive Features Port Orford-Cedar (POC): This species is restricted to the Klamath Mountains and the adjacent S. Oregon Coast Figure 3—Adorni RNA Ranges. Throughout much of its range it is threatened by root rot disease (Phytophthora lateralis), and suitable areas for protection are needed. The area is representative of the low-elevation, mesic portion of the W. Klamath Mountains. In comparison with Upper Goose Creek (#91), however, it is less mesic, and such species as Rhododendron macrophyllum, western hemlock (Tsuga heterophylla), and giant chinquapin (Chrysolepis chrysophylla) are rare or absent. POC is more widespread in the forest away from drainage bottoms than at Upper Goose Creek. In comparison to Cedar Basin (#15), L.E. Horton (#50), and Rock Creek Butte (#70) candidate RNAs, Adorni is a warmer, less montane environment largely without ultramafic substrate. POC in Adorni is less restricted to stream courses than those in Cedar Basin, L.E. Horton, and Rock Creek Butte. Rare Plants: Erythronium citrinum and Lilium rubescens are members of CNPS List 4 species. Large Tanoak (Lithocarpus densiflorus): A small area in the SW. part of the site has several exceptionally large specimens of tanoak. -
Frankia Diversity in Host Plant Root Nodules Is Independent of Abundance Or Relative Diversity of Frankia Populations in Corresponding …
Frankia Diversity in Host Plant Root Nodules Is Independent of Abundance or Relative Diversity of Frankia Populations in Corresponding … Frankia Diversity in Host Plant Root Nodules Is Independent of Abundance or Relative Diversity of Frankia Populations in Corresponding Rhizosphere Soils Seifeddine Ben Tekaya, Trina Guerra, [...], and Dittmar Hahn ABSTRACT Actinorhizal plants form nitrogen-fixing root nodules in symbiosis with soil-dwelling actinobacteria within the genus Frankia, and specific Frankia taxonomic clusters nodulate plants in corresponding host infection groups. In same-soil microcosms, we observed that some host species were nodulated (Alnus glutinosa, Alnus cordata, Shepherdia argentea, Casuarina equisetifolia) while others were not (Alnus viridis, Hippophaë rhamnoides). Nodule populations were represented by eight different sequences of nifH gene fragments. Two of these sequences characterized frankiae in S. argentea nodules, and three others characterized frankiae in A. glutinosa nodules. Frankiae in A. cordata nodules were represented by five sequences, one of which was also found in nodules from A. glutinosa and C. equisetifolia, while another was detected in nodules from A. glutinosa. Quantitative PCR assays showed that vegetation generally increased the abundance of frankiae in soil, independently of the target gene (i.e., nifH or the 23S rRNA gene). Targeted Illumina sequencing of Frankia-specific nifH gene fragments detected 24 unique sequences from rhizosphere soils, 4 of which were also found in nodules, while the remaining 4 sequences in nodules were not found in soils. Seven of the 24 sequences from soils represented >90% of the reads obtained in most samples; the 2 most abundant sequences from soils were not found in root nodules, and only 2 of the sequences from soils were detected in nodules. -
Nr 222 Native Tree, Shrub, & Herbaceous Plant
NR 222 NATIVE TREE, SHRUB, & HERBACEOUS PLANT IDENTIFICATION BY RONALD L. ALVES FALL 2014 NR 222 by Ronald L. Alves Note to Students NOTE TO STUDENTS: THIS DOCUMENT IS INCOMPLETE WITH OMISSIONS, ERRORS, AND OTHER ITEMS OF INCOMPETANCY. AS YOU MAKE USE OF IT NOTE THESE TRANSGRESSIONS SO THAT THEY MAY BE CORRECTED AND YOU WILL RECEIVE A CLEAN COPY BY THE END OF TIME OR THE SEMESTER, WHICHEVER COMES FIRST!! THANKING YOU FOR ANY ASSISTANCE THAT YOU MAY GIVE, RON ALVES. Introduction This manual was initially created by Harold Whaley an MJC Agriculture and Natural Resources instruction from 1964 – 1992. The manual was designed as a resource for a native tree and shrub identification course, Natural Resources 222 that was one of the required courses for all forestry and natural resource majors at the college. The course and the supporting manual were aimed almost exclusively for forestry and related majors. In addition to NR 222 being taught by professor Whaley, it has also been taught by Homer Bowen (MJC 19xx -), Marlies Boyd (MJC 199X – present), Richard Nimphius (MJC 1980 – 2006) and currently Ron Alves (MJC 1974 – 2004). Each instructor put their own particular emphasis and style on the course but it was always oriented toward forestry students until 2006. The lack of forestry majors as a result of the Agriculture Department not having a full time forestry instructor to recruit students and articulate with industry has resulted in a transformation of the NR 222 course. The clientele not only includes forestry major, but also landscape designers, environmental horticulture majors, nursery people, environmental science majors, and people interested in transforming their home and business landscapes to a more natural venue. -