IHC and ISH 2010 Product Range
TM Including NovocastraTM and Bond Reagents
Living up to Life HOW TO USE THIS CATALOG
Products in this catalog are listed alphabetically in sections (Bond™, Novocastra™ and Origin™ ). To find a product either use the contents page to locate the appropriate section and then go directly to the product, or use the product name index at the back of the catalog.
All Novocastra products are listed with their product Origin products include the product code, number of tests code and additional information (e.g. clone designation), and the clone. All Origin product codes start with “ORG”. product type (Kit, Primer Set, etc.), and volume or a guide All antibodies, unless otherwise stated, are reactive with to the number of tests in one or more of the recommended their respective human antigens. All monoclonal antibodies applications. The fi rst letters of the product code indicate are murine and most polyclonal antibodies are of rabbit the product type: origin unless otherwise stated. NCL Concentrated Primary Antibody, Probe or Species cross-reactivity and more detailed western blotting Miscellaneous Product information that is not indicated in either the product text or RTU Ready-to-use Primary Antibody datasheet may be obtained at www.leica-microsystems.com RE Manual Detection (Complete System or or by contacting your local customer support (refer to back Individual Component) or Ancillary Reagent page for further details). As a result of customers’ research, Bond ready-to-use products include the product code, clone the cross-reactivity information is updated regularly, and designation (where appropriate) and product size. unless indicated on the datasheet, should be treated only as PA Bond Ready-to-use Primary Antibody a guide. Bond and Novocastra products are designed for use in immunohistochemical, microbiological/virological, in situ PB Bond ISH Probe hybridization, and other techniques. AR Bond Ancillary Reagent All antibodies are described as monoclonal or polyclonal, DS Bond Detection System and most are available in a range of formats, e.g., lyophilized IVD In Vitro Diagnostic use (freeze-dried), liquid, and ready-to-use. RUO Research Use Only Human Colonic Carcinoma: immunohistochemical staining for PMS2 using NCL-L-PMS2
Reagent products are designed primarily for immuno- P (Enzyme+HIER) Paraffi n sections with enzyme histochemical or in situ hybridization use. Other digestion followed by heat induced recommended applications may also be indicated. The epitope retrieval recommended code letters beside each product provide a key that briefl y P (Enzyme/HIER) Paraffi n sections with enzyme describes the use of the product. digestion or heat induced epitope F Frozen sections with no pretreatment retrieval recommended – optimum P Paraffi n sections with no pretreatment to be determined by pretreatment end user P (HIER) Paraffi n sections with heat induced W Western blotting epitope retrieval recommended I Immunofl uorescence P (Enzyme) Paraffi n sections with enzyme C Flow cytometry digestion recommended E Electron microscopy O Other applications defi ned in text
Note: There is a quick reference guide to the key symbols located on the bottom of each right-hand page.
/ 1 LEICA MICROSYSTEMS BIOSYSTEMS DIVISION
The Biosystems Division of Leica Microsystems is a world wide organization with an international sales and support network backed by in-house reagent and automation development. This comprehensive coverage and knowledge makes Leica Microsystems’ Biosystems Division the ideal partner for advanced staining.
Leica Microsystems Global Representation
/ 2 www.leica-microsystems.com CONTENTS
New Products ...... 4 Total Histology Solutions ...... 6 Lean Consulting ...... 8
BondTM Reagents ...... 9
Detection Systems ...... 12
Ancillary Reagents ...... 13
Bond Ready-to-Use Primary Antibodies ...... 15
Bond Ready-to-Use ISH Probes ...... 45
Consumables ...... 48 Novocastra Primary Antibodies...... 51 Novocastra Manual Detection Systems ...... 173 Novocastra Epitope Retrieval Reagents and Buffers . . 179 Novocastra ISH Reagents/Probes ...... 183 Novocastra Primer Sets ...... 187
OriginTM Reagents ...... 191 Product Name Index...... 197 Reagent Ordering Information ...... 222
Novocastra Science™ – experience, innovation and support that delivers quality reagents for consistent staining
/ 3 NEW PRODUCTS
Clinicians and researchers continually strive for better patient outcomes through the discovery of new diagnostic tools. Leica Microsystems aids this important work by continually releasing new, useful antibodies and probes to clinical and research laboratories. This catalog includes 37 new products released in the past 12 months, and with more releases planned for 2010, Leica Microsystems is helping make sure patients receive better care through the latest diagnostic advances.
NOVOCASTRA ANTIBODIES
NCL-L-AMACR Alpha-Methylacyl-CoA Racemase (AMACR, p504s) NCL-L-CALCITONIN Calcitonin NCL-L-CD99-187 CD99 NCL-L-CD123 CD123 NCL-L-FRalpha Folate Receptor Alpha NCL-L-FPGS Folylpolyglutamate Synthetase NCL-L-Hpylori Helicobacter pylori NCL-L-HIG2 Hypoxia Inducible Gene 2 Protein NCL-L-IgG Immunoglobulin G NCL-L-InhibinA Inhibin Alpha NCL-KAP-581 Kappa Light Chain NCL-L-LAM-578 Lambda Light Chain NCL-L-PMS2 Mismatch Repair Protein (PMS2) NCL-L-MUM1 Multiple Myeloma Oncogene 1 (MUM-1) NCL-MSA-594 Muscle Specifi c Actin
/ 4 www.leica-microsystems.com BOND RTU ANTIBODIES BOND DETECTION SYSTEMS
PA0117 Bcl-2 Oncoprotein DS9390 Bond Polymer Refi ne Red Detection PA0083 Beta-Catenin DS9455 Bond Research Detection PA0088 CD138 (Syndecan 1) PA0114 Cytokeratin 17 PA0238 Galectin-3 PA0681 Gastrin PA0594 Glucagon PA0110 Inhibin (alpha) PA0230 Ki67 PA0655 Luteinizing Hormone PA0610 MLH1 (Mismatch Repair Protein) PA0048 MSH2 (Mismatch Repair Protein) PA0129 Multiple Myeloma Oncogene 1 (MUM-1) PA0391 Muramidase (Lysozyme) PA0491 Myeloperoxidase PA0996 Negative Control (Mouse) PA0777 Negative Control (Rabbit) PA0371 Neurofi lament 200kD PA0934 Oct 3/4 PA0093 Tartrate-Resistant Acid Phosphatase (TRAP)
/ 5 TOTAL HISTOLOGY SOLUTIONS
It’s about patients, and everything you need to help them through their journey. Leica Total Histology is all elements of tissue-based pathology brought together – instruments and consumables, history PATIENT and education, support, and innovation. Now, instruments and consumables form complete systems, each individual step is considered part of a single inclusive process and one partner can support the entire histology workflow. With Total Histology, it’s time to advance workflows, enhance diagnostic clarity and deliver better patient care. PATHOLOGIST
Microscopes
Coverslipping/ Staining
/ 6 Workstations www.leica-microsystems.com Specimen Consumables Identification Cryosectioning
Tissue Processing
TOTAL HISTOLOGY
Embedding
Paraffin Sectioning
Routine Staining
™ Bond Fully / 7 Automated IHC Novocastra™ and ISH Reagents LEAN CONSULTING
Improve productivity, enhance quality and lower costs with Leica Microsystems’ Lean Histology™ Consulting Service. Leica Microsystems’ Lean Consultants create a roadmap for your laboratory that can improve processes and increase efficiency. Along with an expert, in-person workflow evaluation, Lean Consultants work with you to identify and apply the best combination of people, products, and processes for sustainable improvements that increase performance and aid better patient care through higher quality and faster results.
Please consult your Leica Microsystems representative for availability in your region.
/ 8 www.leica-microsystems.com Bond osmbe...... 48 ...... Consumables...... 45 Probes. ISH Ready-to-Use Bond ..15 Antibodies. Primary Ready-to-Use Bond .13 . Reagents Ancillary ..12 Systems Detection Contents staining. high-quality consistent, deliver you titration; and dilution mixing, the done We’ve “Start”. click and reagent primary your load easier. been Simply never have ISH and IHC brilliant range, ready-to-use Bond the With BOND BOND REAGENTS REAGENTS TM READY-TO-USE PRIMARY PRIMARY READY-TO-USE TM W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence O E te applications Other lcrnmicroscopy Electron /9
Bond Bond o ealdifraino l rdcspes ii u website: our visit please www.leica-microsystems.com products all on information detailed For /10 BOND and flexibility same quality throughput, –lower available,Also Bond-max care tissue and total reagents Novocastra –superior Quality • and maintenance times reagent costs cut ismore: – less Efficiency • stainers IHC/ISH generation previous than faster 50% Speed–upto • care. patient and better rapid turnaround workflows, –Lean matters really what helpsyoudeliver Bond-III productivity. greater slides with high-quality more finish Quickly MATTERS REALLY WHAT –DELIVERING BOND-III ™ FULLY AUTOMATED IHC AND ISH IHC AND AUTOMATED FULLY hsctlgi o o s nteUSA. the in use for not approval. is regulatory catalog to This subject are catalog this in Products • Complete – all the reagents required to perform up to 60 tests on the Bondsystem onthe tests 60 upto perform to required reagents –all the Complete • cell and four lines thickness section interferometer-verified cell slides with –control Consistent • staining the standardizing Bondsystem, onthe run reagents –ready-to-use automated Fully • ORACLE isbeingconsidered treatment (trastuzumab) Herceptin® whom for patients from tissue breast in status HER2 of accurate the andfor assessment dependable robust IHCtest automated, A fully CHOICE THERANOSTIC WISE THE • 15 positive in-house tissue controls with HER2 Primary Antibody Primary HER2 with controls tissue in-house 15positive • Antibody Primary HER2 with Slides Control 15HER2 • Control Negative HER2 with slides test 60 • Antibody Primary HER2 with slides test 60 • Oracle (including 2+) provide a consistent, comprehensive control method for assessing assay performance performance assay assessing for method control comprehensive aconsistent, provide 2+) (including and repeats variation staining andprocess reducing time, hands-on ™ HER2 Bond HER2 ™ ™ IHC System – System IHC HER2 BOND HER2 TA9145 ™ IHC SYSTEM (TA9145) IHC SYSTEM 2. When used with standard Bond system ancillary reagents ancillary system Bond standard with used When 2. 1. See 1. See Herceptin W P F etr blotting Western Paraffin Frozen ® package insert C I lwcytometry Flow Immunofluorescence 2 . O E te applications Other lcrnmicroscopy Electron 1 . /11
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Solution Retrieval Epitope Bond and (SPT24, Detection Factor-1 Refine Transcription Polymer Thyroid Bond ready-to-use using Bond PA0364), with TTF-1 for stained lung Human Epitope Bond with used per-formance. optimize when to out carried optimally be prevent should Testing stain Thyroid 1. to ready-to-use Solution Retrieval Bond technology as Factor-1, Covertile such Transcription antibodies, Bond's reduced Some a paraffin- utilizes evaporation. has it reagent formalin-fixed, been and as have sections on temperature on that gentle use tissue is boiling solution within for The epitopes fixation. is during expose masked to 1 sections Solution tissue embedded Retrieval Epitope Bond the on (HIER) Application retrieval epitope 6.0 pH heat-induced based system. for citrate Bond ready-to-use, automated specifically L is 1 a It is solution. 1 Solution Retrieval Epitope Bond Components Use Diagnostic Vitro In For AR9961 L 1 1 Solution Retrieval Epitope Bond P P oksctmr muoitceia tiigwt odrayt-s lh eorti (C3) Detection. 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Refine Polymer Bond using (5A4) Anaplastic Kinase ready-to-use Lymphoma Bond with staining immunohistochemical lymphoma: Anaplastic third 54. page to one refer concentrate, which Novocastra non- a of as all available adults, Also of young p80. percent translocation. and protein, gene 25 NPM-ALK children the the approximately carries in to the for chromosomal rise lymphomas of account giving Hodgkin's domain a lymphomas gene, cytoplasmic cell (ALK) the contain Large of to kinase part cases joined lymphoma encoding gene gene anaplastic hybrid these (NPM) a nucleophosmin in of results in the This occur (p23;q35). proportion extranodal t(2;5) to and translocation the cutaneous tend A with and large cells associated antigen be tumor involvement. of may CD30 These and composed express individuals (EMA). younger usually to antigen reported is membrane are epithelial (ALCL) which, Lymphoma cells pleomorphic Cell Large Anaplastic Use Diagnostic Vitro In PA0306 For ready-to-use Bond mL 7 5A4 Clone Kinase Lymphoma Anaplastic hsctlgi o o s nteUSA. the in use for not approval. is regulatory catalog to This subject are catalog this in Products (HIER) P P osl muoitceia tiigwt odrayt-s c- bl210D)uigBond using Detection. 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(TG14) Refine BOB-1 Polymer ready-to-use Bond with staining immunohistochemical Lymphoma: 59. page to refer variable. concentrate, be Novocastra to cells. a reported plasma as been available has and Also tumors cells cell B B The in center tissues. BOB-1 lymphoid germinal of be expression reactive in The to in found found reported is populations been expression cell It the strongest has B to BOB-1 protein. all contributes motifs. in and coactivator detectable octamer factors of transcriptional transcription activity OCT2 specific transcriptional and OCT1 lymphocyte and with OBF-1 a interacts as known also is (BOB-1), protein-1 OCA-B, binding octamer specific cell B Use Diagnostic Vitro In PA0558 For ready-to-use Bond mL 7 TG14 Clone (BOB-1) Protein-1 Binding Octamer Specific Cell B Bond Ready-to-UsePrimaryAntibodies New! 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Product the expressed differentiation in the cells kD in interdigitating useful and 43–49 be cells to Langerhans’ shown of of been has protein expression CD1a thymocytes. a is CD1a Use Diagnostic Vitro In PA0235 For ready-to-use Bond mL 7 MTB1 Clone CD1a osl muoitceia tiigwt odrayt-s D 1F1 sn odPolymer Bond using Detection. (11F11) Refine CD2 ready-to-use Bond with staining immunohistochemical Tonsil: the 70. in page to panel refer concentrate, antibody Novocastra a an as of available Also part as disorders. use cell T for of recommended characterization is (11F11) CD2 the Information mediating Specific Product in cells antigen-presenting important with molecule, thymocytes cells. and target accessory cells and T an activated is of adhesion antigen CD2 The Use Diagnostic Vitro In PA0271 For ready-to-use Bond mL 7 11F11 Clone CD2 Detection. Refine W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence (HIER) P (HIER) P O E te applications Other lcrnmicroscopy Electron /17
Bond Bond o ealdifraino l rdcspes ii u website: our visit please www.leica-microsystems.com products all on information detailed For /18 Detection. CD4 Refine ready-to-use Polymer Bond Bond with using staining (4B12) immunohistochemical cells: helper/inducer cell T Tonsil, cell T 71. page cutaneous to refer concentrate, of Novocastra a cases as positive. available CD4 Also generally Most also is monocytes. leukemia/lymphoma HTLV-1 cell and on T antigen adult CD4 associated level the express fungoides, lower lymphocytes mycosis subset including blood cell lymphoma, a T peripheral a at of on expressed percent and is 45 antigen a CD4 representing with The glycoprotein (helper/inducer) kD. transmembrane 59 chain of single weight a molecular is (T4) molecule CD4 The Use Diagnostic Vitro In PA0368 For ready-to-use Bond mL 7 4B12 Clone CD4 Polymer Bond using (LN10) Detection. CD3 Refine ready-to-use Bond with staining immunohistochemical Tonsil: 70. page to refer concentrate, Novocastra a as available Also one antibodies. as disorders of cell panel T a of of characterization component the in formalin-fixed, employed be on can (LN10) stain CD3 unrivaled an produce tissue. and supercede paraffin-embedded to PS1 designed clone specifically been Novocastra has (LN10) CD3 the ready-to-use Bond of one in represent detected Information Specific to first lineage. Product cell is thought T It the is to differentiation. commitment appearance cell of signs its T earliest of and marker thymocytes a early is antigen CD3 The Use Diagnostic Vitro In PA0553 For ready-to-use Bond mL 7 LN10 Clone CD3 (HIER) P (HIER) P osl muoitceia tiigwt odrayt-s D 47 sn odPolymer Bond using (4C7) Detection. CD5 Refine ready-to-use Bond with staining immunohistochemical Tonsil: 71. page to the refer for concentrate, Novocastra panels a antibody as available in Also useful lymphomas. be cell however B to lymphomas, small cells, found of B diagnosis been B and differential have T of CD5 some subset in to a described antibodies been in has and expression thymocytes CD5 reported of zones. is mantle percent It the cells. 95 in T primarily in located on mainly expressed expressed be is to CD5 tissue lymphoid normal In Use Diagnostic Vitro In For PA0168 ready-to-use Bond mL 7 4C7 Clone CD5 osl muoitceia tiigwt odrayt-s D L1)uigBn Polymer Bond using (LP15) Detection. CD7 Refine ready-to-use Bond with staining immunohistochemical Tonsil: 72. page to refer concentrate, Novocastra a as available and Also cells killer natural be been most identified, cells, to has T fully reported blood receptors thymocytes. is peripheral been of antigen gamma/delta majority The yet CD7 the with on activation. differentiation. not found cells mAb-induced has throughout on T molecule based persist of proposed CD7 activation to the the marker of although early role the antigen is and only CD7 and lymphocytes. function the kD in expressed 40 also be of to is antigen glycoprotein specific membrane-bound cell a T earliest is molecule CD7 The Use Diagnostic Vitro In For PA0266 ready-to-use Bond mL 7 LP15 Clone CD7 hsctlgi o o s nteUSA. the in use for not approval. is regulatory catalog to This subject are catalog this in Products (HIER) P (HIER) P osl muoitceia tiigwt odrayt-s D0(66 sn odPolymer Bond Detection. using (56C6) Refine CD10 ready-to-use Bond with staining immunohistochemical Tonsil: 73. page to refer cell concentrate, Novocastra B a as small available of Also diagnosis leukemia. lymphoblastic differential of the subtyping the for and follicular lymphoma recommended is and (56C6) CD10 Burkitt's lymphoblastic, leukemia. Information myeloid of Specific chronic Product with patients cells of cells antigen on the CD10 on and origin. identified lymphomas nonlymphoid and normal and been of lymphoid variety has both wide a of on of tissues expressed number neoplastic is a It inactivates peptides. which active metalloendopeptidase biologically a is antigen CD10 The Use Diagnostic Vitro In PA0270 For ready-to-use Bond mL 7 56C6 Clone CD10 Polymer Bond using (4B11) Detection. CD8 Refine ready-to-use Bond with staining immunohistochemical Tonsil: that antibodies 72. page of to refer concentrate, panel Novocastra a a as available using Also characterized be sub- (4B11). can CD8 and include disorders thymocytes of cell cells. marrow percent T bone and also 80 cells has cells, null It blood killer peripheral lymphocytes. of natural blood populations cells, peripheral on cytotoxic/supressor of normal reported percent of been 20-30 sub-set a up on make found which is antigen CD8 The Use Diagnostic Vitro In PA0183 For ready-to-use Bond mL 7 4B11 Clone CD8 (HIER) P (HIER) P loaalbea ooatacnetae ee opg 73. page to refer concentrate, Novocastra a identifi- as tissues. available and in Also lineage malignancies cell macrophage/dendritic hematological the panel of of a cells diagnosis of of part cation the as in use for antibodies recommended of is (5D11) CD11c ready-to-use Bond Information hairy Specific cells. including Product dendritic been lines interdigitating cell and has of lymphoid proportion antigen macrophages a cells, and T CD11c tissue leukemia activated cell granulocytes. proteins on cells, NK adhesion of levels on of staining high described family weak with integrin with cells, leukocyte monocytes normal the of in member expressed a is CD11c Use Diagnostic Vitro In PA0554 For ready-to-use Bond mL 7 5D11 Clone CD11c sn odPlmrRfn Detection. Refine (Carb-1) Polymer CD15 Bond ready-to-use using Bond with staining immunohistochemical lymphoma: Hodgkin’s some and 74. disease page to refer Hodgkin's concentrate, Novocastra a of as available Also cells Sternberg Reed also circulating leukemias. is antigen of 90 on CD15 percent The on lymphocytes. 60 expressed expressed normal to from be absent 30 to is granulocytes, and reported monocytes human is circulating X-hapten, of as percent known also antigen, CD15 Use Diagnostic Vitro In PA0039 For ready-to-use Bond mL 7 Carb-1 Clone CD15 Detection. using Refine (5D11) CD11c Polymer ready-to-use Bond Bond with staining immunohistochemical leukemia: cell Hairy W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence (HIER) P (HIER) P O E te applications Other lcrnmicroscopy Electron /19
Bond Bond o ealdifraino l rdcspes ii u website: our visit please www.leica-microsystems.com products all on information detailed For /20 Polymer Bond using (MJ1) Detection. CD20 Refine ready-to-use Bond with staining immunohistochemical Tonsil: cell B 75. page of to refer characterization concentrate, Novocastra the a as in available antibodies. Also of use panel a for of component one recommended as disorders is (MJ1) from CD20 cells B Information Specific malignant Product leukemias and acute marrow, leukemias. normal lymphocytic bone chronic tonsil, on and spleen, and node, activation expressed lymph cell blood, B during is peripheral receptor a It as act to differentiation. thought is antigen CD20 The Use Diagnostic Vitro In PA0906 For ready-to-use Bond mL 7 MJ1 Clone CD20 Bond Detection. using Refine (BT51E) CD19 Polymer ready-to-use Bond with staining immunohistochemical tonsil: Normal 74. page to refer concentrate, granulocytes. Novocastra normal a on as or available cells lines. Also T cell on dendritic B present follicular a stabilized not all on is most with found by antigen and also CD19 expressed lineage glycoprotein The is myelomonocytic be It of to membrane cells. cells reported early B integral is cells, early including antigen I cells This B type kD. normal 90 a of is weight molecular molecule CD19 The Use Diagnostic Vitro In PA0843 For ready-to-use Bond mL 7 BT51E Clone CD19 (HIER) P (HIER) P edii el n nfliua n atecl ypoa,mature lymphomas, 75. cell page to refer mantle concentrate, Novocastra a and as available follicular Also follicular in expressed in and be lymphomas. monocytes and and to leukemias lymphocytes, reported cells T also on is dendritic acute antigen absent cell CD21 T is of granulocytes. subset but a be in to leukemias and reported leukemias is lymphocytic lymphocytic antigen chronic CD21 cell virus. B Epstein-Barr cell in third for B found receptor the the is a of of cells, interior as the acts B fragment to and mature C3d signals on growth-promoting the transmitting present for in molecule, involved receptor CD21 the The component. as weight complement functions molecular of which glycoprotein membrane kD, integral 140 I type a is antigen CD21 Use Diagnostic Vitro PA0171 In For ready-to-use Bond mL 7 2G9 Clone CD21 odPlmrRfn Detection. Refine using (FPC1) Polymer CD22 Bond ready-to-use Bond with staining immunohistochemical lymphoma: Follicular 75. page to refer concentrate, acute and Novocastra a granulocytes cell as leukemias, available cell Also non-T T cells, and T blood leukemias. leukemias peripheral cell monocytes. on hairy myeloid on absent expressed is on strongly It is reported expressed and also leukemias is weakly lymphoblastic antigen CD22 be is differentiation. expression upon antigen to lost Surface be expression. may same antigen and the CD19 variable to approximately the at reported of differentiation that is the lymphocyte as antigen in stage cell CD22 B and the in membrane of early Expression the appear independently lymphocytes. on two B of both heterodimer of a present cytoplasm is chains It kD. glycoprotein with 140 glycoprotein to expressed membrane 130 integral of 1 weight type molecular a a is (BL-CAM) antigen CD22 The Use Diagnostic Vitro PA0249 In For ready-to-use Bond mL 7 FPC1 Clone CD22 Polymer Bond using (2G9) Detection. CD21 Refine ready-to-use Bond with staining immunohistochemical Tonsil: hsctlgi o o s nteUSA. the in use for not approval. is regulatory catalog to This subject are catalog this in Products (HIER) P (HIER) P D5(C)uigBn oye eieDetection. ready-to-use Refine Bond Polymer with Bond staining using immunohistochemical (4C9) cells: CD25 NK and cells T activated Tonsil, 125. page to refer concentrate, auto- Novocastra a neoplasia, as available lymphoid Also conditions, associated rejection. be allograft malignant to and diseases reported immune and is HTLV- expression inflammatory receptor on non-activated IL-2 cells, with cells. expressed T null be resting and thymocytes, cells to on B and precursors reported absent myeloid is cells, is It B oligodendrocytes. EBV-transformed and not cells, B cells is and receptor T T transformed IL-2 IL-2. on of functions. for capable found cytotoxic receptors is exclusively and which affinity population suppressor high cell helper, T expresses mediating the cells expanding proliferate, T of cells to of activation These response the In subpopulation secreted. Following a and synthesized kD. this rapidly single-chain 55 is (IL-2) a of interleukin-2 is weight cells T receptor, molecular interleukin-2 a of with subunit glycoprotein alpha the antigen, CD25 Use Diagnostic Vitro In PA0305 For ready-to-use Bond mL 7 4C9 Clone CD25 Polymer Bond using Detection. (1B12) Refine CD23 ready-to-use Bond with staining immunohistochemical Tonsil: 76. page to refer concentrate, Novocastra a as available leukemias). Also lymphocytic (chronic differential present CLL be the and to reported for lymphomas been lymphocytic panel has small it antibody in where lymphomas an cell B origin, within small of use diagnosis hemopoietic for recommended of is cells CD23 other tissues. on Information normal Although Specific nonlymphoid Product expressed of cells. range large dendritic be a follicular from to absent of is expression known sub-population mantle on a is expressed on variably CD23 is and receptor, cells IgE affinity zone low the antigen, CD23 Use Diagnostic Vitro PA0169 In For ready-to-use Bond mL 7 1B12 Clone CD23 (HIER) P (HIER) P loaalbea ooatacnetae ee opg 76. page to Hodgkin's refer concentrate, and Novocastra a lymphomas as available cell Also large anaplastic of Lymphomas. panel identification antibody an the of part as for use for recommended cellular is CD30 of ready-to-use Bond regulation the in Information part Specific Product a play lymphoblasts. receptor activated a may of as transformation It act and to CD30L. growth known ligand glycoprotein, chain cytokine single for a is antigen CD30 The Use Diagnostic Vitro In PA0153 For ready-to-use Bond mL 7 1G12 Clone PA0790 ready-to-use Bond mL 7 JCM182 Clone CD30 spau:imnhsohmclsann ihBn ed-oueC3 11)uigBond using (1A10) CD31 ready-to-use Detection. Bond Refine with Polymer staining immunohistochemical Esophagus: 77. page to refer concentrate, Novocastra a as available Also of tissues. assessment neoplastic the in in invasion use for vascular recommended is (1A10) CD31 ready-to-use Bond Information Specific Product wound metastases. and and growth thrombosis tumor it for angiogenesis, essential that during is suggest events Angiogenesis antigen healing. interactive stabilize CD31 to in of serve involved properties may The is and monolayer. contact cell endothelial extracellular endothelial in cell- the cell- role endothelial an of a mediate plays typical to thought has adhesion, subclass, is to-cell C2 domain of molecule This units glycoprotein molecules. homology adhesion The to-cell Ig-like transmembrane six junction. contains at chain that and intracellular domain cells single B endothelial granulocytes, monocytes, a platelets, the of is surface the on (PECAM-1) expressed antigen CD31 Use Diagnostic Vitro In For PA0250 ready-to-use Bond mL 7 1A10 Clone CD31 (JCM182) CD30 Detection. ready-to-use Refine Bond Polymer Bond with using staining immunohistochemical lymphoma: Hodgkin's W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence (HIER) P (HIER) P (HIER) P O E te applications Other lcrnmicroscopy Electron /21
Bond Bond o ealdifraino l rdcspes ii u website: our visit please www.leica-microsystems.com products all on information detailed For /22 Detection. Refine Polymer with staining immunohistochemical Tonsil: 77. page to refer neoplasias, concentrate, of Novocastra a variety as available a (QBEnd/10). Also CD34 in using hemopoietic leukemias identified lymphoblastic be acute myeloid and can tumors including expression and antigen is CD34 that lymphoid The glycoprotein human transmembrane endothelium. vascular chain and on cells single progenitor expressed a is selectively antigen CD34 The Use Diagnostic Vitro In PA0212 For ready-to-use Bond mL 7 QBEnd/10 Clone CD34 (PWS44) CD33 Detection. Refine ready-to-use Polymer Bond Bond with using staining immunohistochemical sarcoma: Granulocytic 77. page to refer concentrate, Novocastra a as available CD33 Also of distinguishing for leukemias. expression marker lymphoid important the The an from be myeloid to undifferentiated leukemias. demonstrated been lymphoblastic acute has on to antigen acute some restricted absent is and blasts, be CD33 to the leukemias of myeloid expression both reported premyelocytes, that on is reported monocytes, expressed it been has be although It to lineages, granulocytes. continues monocyte then and after It myeloid cells expression. precursor myelomonocytic antigen on appear CD34 to reported is antigen CD33 Use Diagnostic Vitro In PA0555 For ready-to-use Bond mL 7 PWS44 Clone CD33 odready-to-use Bond (HIER) P (HIER) P D4(Bn/0 sn Bond using (QBEnd/10) CD34 ifs ag ellmhm:imnhsohmclsann ihBn ed-oueCD43 ready-to-use Bond with staining immunohistochemical lymphoma: cell B large Diffuse 79. page to refer concentrate, Novocastra a as marker. available leukocyte Also pan a as used be may antibody This Information Specific Product of cytoplasm the in and membrane the on T expressed is antigen CD43 The Use Diagnostic Vitro In PA0938 For ready-to-use Bond mL 7 MT1 Clone CD43 osl muoitceia tiigwt odrayt-s D5(1/9 sn Bond using Detection. (X16/99) Refine CD45 Polymer ready-to-use Bond with staining immunohistochemical Tonsil: 80. of page to tumors refer concentrate, of Novocastra a identification as available the antibodies. Also of in panel a use of part for as origin recommended lymphoid is (X16/99) CD45 Information molecular-weight Specific Product cell high T is the through more It erythrocytes signalling from for exons. or necessary absent receptors. is three but expression five leukocytes, CD45 of human platelets. of of splicing and majority alternative family the on the a present by is generated antigen glycoproteins CD45 The Use Diagnostic Vitro In PA0042 For ready-to-use Bond mL 7 X16/99 Clone CD45 Detection. Refine Polymer Bond using (MT1) egthtrgniywt ad f9 o10k bevdo SDS-PAGE on reported cells. are observed T antigen neoplastic kD CD43 and normal the 140 include expressing to to Cells lines. 90 cell of different between bands with heterogeneity weight el n el fmeodlnae h oeueisl xiismolecular exhibits itself molecule The lineage. myeloid of cells and cells hsctlgi o o s nteUSA. the in use for not approval. is regulatory catalog to This subject are catalog this in Products (HIER) P (HIER) P D6(D6)uigBn oye eieDetection. Refine Polymer ready-to-use Bond Bond using with (CD564) staining CD56 immunohistochemical thyroid: the of carcinoma Medullary 81. page to refer concentrate, Novocastra a as available tumors. Also a neuroendocrine some and and cells CD56 lymphocytes NK The T cells, 5. activated Schwann of chromosome astrocytes, subset on neurons, located on gene expressed of single is splicing antigen a differential from by transcript generated RNA cell isoforms antigen the major CD56 closely-related four The of of cells. consists neural (NCAM) family development between embryogenesis, a interactions in role contact-mediated are a and play molecules to thought adhesion glycoproteins surface cell neural The Use Diagnostic Vitro PA0191 In For ready-to-use Bond mL 7 CD564 Clone CD56 Detection. Refine (UCHL1) Polymer CD45RO Bond ready-to-use using Bond with staining immunohistochemical lymphoma: Follicular cell 81. page T to refer concentrate, of Novocastra a majority as available Also the expression. cytoplasmic on the exhibit macrophages of and tissue expression whereas surface antigen thymocytes show positive granulocytes CD4 of and of Monocytes percent malignancies. percent 37 lymphocytes, expressed 80 T be to blood lymphocytes, reported peripheral is of CD45, percent of 48 isoform on kD 180 a molecule, CD45RO The Use Diagnostic Vitro In PA0146 For ready-to-use Bond mL 7 UCHL1 Clone CD45RO (HIER) P (HIER) P acm:imnhsohmclsann ihBn ed-oueC5 N-)uigBond using Detection. (NK-1) Refine CD57 Polymer ready-to-use Bond with staining immunohistochemical Sarcoma: 82. page to refer concentrate, Novocastra on a of expressed as available not subset Also is a molecule CD57 are The platelets. grafts disease. or of proteins host erythrocytes rejection the versus CD8 in graft role and acute a in play CD57 cells activity These killer cells. co-express T natural suppressor/cytotoxic glycoprotein. which with myelin-associated cells cells expressing mononuclear of Many cells subset neuroectodermal 110 a on of on weight and molecular found a is has It HNK-1, as kD. known also glycoprotein, CD57 The Use Diagnostic Vitro PA0443 In For ready-to-use Bond mL 7 NK-1 Clone CD57 osl muoitceia tiigwt odrayt-s D1(f)uigBn Polymer Bond using (2f2) Detection. CD61 Refine ready-to-use Bond with staining immunohistochemical Tonsil: 82. page to refer concentrate, CD61 Novocastra a detecting as tissues. available in neoplastic Also use and normal for of variety recommended a in is expression (2f2) protein CD61 ready-to-use Bond (integrin IIb/IIIb Information aVß3). Specific (integrin glycoprotein receptor Product platelet vitronectin the the form to form CD51 cells. with to and endothelial CD41 aIibß3) on with and expressed is combines macrophages that CD61 glycoprotein monocytes, a is megakaryocytes, GPIIIa, as platelets, known also antigen, CD61 The Use Diagnostic Vitro PA0308 In For ready-to-use Bond mL 7 2f2 Clone CD61 W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence (HIER) P (HIER) P O E te applications Other lcrnmicroscopy Electron /23
Bond Bond o ealdifraino l rdcspes ii u website: our visit please www.leica-microsystems.com products all on information detailed For /24 Detection. Refine Polymer ready-to-use Bond Bond using with (11E3) staining CD79a immunohistochemistry transition: cell cell-plasma B Tonsil, some in and lymphomas 84. cell page to refer B concentrate, Novocastra lines, a lines. as cell cell available T Also or The B myeloid in component. type, present not intracellular is cell leukemias It an acute myelomas. B of as majority the precursor found in the expressed of is until be to persists it reported and is where antigen maturation, CD79a early stage to stage, CD79b reported cell cell and is pre-B plasma component CD79a the CD79a at subunits, The appear receptor. two first antigen the cell B immunoglobulins the these constitute with on immunoglobulins cells, membrane-bound with B associated is complex CD79 The Use Diagnostic Vitro PA0192 In For ready-to-use Bond mL 7 11E3 Clone CD79a Bond using Detection. (514H12) Refine CD68 Polymer ready-to-use Bond with staining immunohistochemical Tonsil: 83. page neoplastic to refer and concentrate, Novocastra normal a of as available variety Also a in identified (514H12). CD68 be using can tissues antigen CD68 The tubules. large Information NK renal Specific and cells, Product and liver T in and stimulated basophils mem- carcinomas, in and the sarcomas reported neutrophils, lymphomas, been extent, cells, has monocytes, lesser been expression primarily a CD68 macrophages, lymphocytes. has to which of and, molecule, granules branes intracellular cytoplasmic an with is associated antigen CD68 The Use Diagnostic Vitro In PA0273 For ready-to-use Bond mL 7 514H12 Clone CD68 (HIER) P (HIER) P wn’ acm:imnhsohmclsann ihBn ed-oueC9 1E)using (12E7) CD99 ready-to-use Bond Detection. Refine with Polymer staining Bond immunohistochemical sarcoma: Ewing’s 85. on page to expressed refer concentrate, strongly Novocastra some a be as on available Also to and tumors. neuroectodermal testis reported peripheral primitive the cortical is and cells of antigen sarcoma on cells Ewing's the CD99 with Sertoli expressed of cells. cells cells, formation granulosa be islet endothelial rosette on pancreatic expressed in to also most involved is reported ovary, It is erythrocytes. is and human HuLy- or lymphocytes antigen 12E7, sheep E2, T CD99 MIC2, and as FMC29. known thymocytes also or is glycoprotein, kD m6 32 a antigen, CD99 Use Diagnostic Vitro PA0509 In For ready-to-use Bond mL 7 12E7 Clone CD99 lsayoa muoitceia tiigwt odrayt-s D3 (Synedcan-1) CD138 Detection. Refine ready-to-use Polymer Bond Bond using with (MI15) staining immunohistochemical Plasmacytoma: differentiation, is immunohistochemistry and in marker during cells. a plasma morphology as event of CD138 quantification early CD138 the of epithelial utility an of major preserved the close be loss however, a to with shows The expression reported antigen epithelia. association CD138 is whereas simple as cells carcinogenesis well and cervical atypical as cells stratified from plasma in expression Ig-producing and expressed cells such B glycoprotein cells being immature lymphoid sulphate normal cells, in heparan pre-B differentiation as transmembrane of stages a distinct at is expressed molecule CD138 The Use Diagnostic Vitro In PA0088 For ready-to-use Bond mL 7 MI15 Clone 1) (Syndecan CD138 hsctlgi o o s nteUSA. the in use for not approval. is regulatory catalog to This subject are catalog this in Products New! (HIER) P P odPlmrRfn Detection. using Refine (5H7) A Polymer Chromogranin Bond ready-to-use Bond with staining immunohistochemical Pancreas: 91. page to refer concentrate, Novocastra a as available origin. neuroendocrine Also of tumors of of part identification as the for use antibodies for of recommended panel is a (5H7) A Chromogranin ready-to-use Bond carcinomas, Information Specific thyroid Product medullary carcinoids. adenomas, and phaeochromocytomas, pituitary tumors cell as tumors, Merkel such expression tumors cell A Chromogranin neuroendocrine widely thyroid. islet islet in the be reported gland, of cells to been pituitary C has anterior reported and endocrine pancreas medulla, is human the adrenal of of that cells gland, granules protein parathyroid secretory in acidic eg and cells; kD tissues 68 neural in a expressed is A Chromogranin Use Diagnostic Vitro PA0430 In For ready-to-use Bond mL 7 5H7 Clone A Chromogranin Detection. using Refine (AMT28) Polymer CDX2 Bond ready-to-use Bond with staining immunohistochemical epithelium: Bowel 89. page to refer concentrate, Novocastra a as as available from lines. Also stomach, cell transition cancer the the gastric with differentiated of poorly decreases carcinogenesis to progressively differentiated with well mRNA cancers associated CDX2 colorectal be of sporadic also expression some may of reported CDX2 progression been and has the CDX2 the towards of but loss all contribute In The in to intestine. crypts. occur developing the to the in reported of and is portions expression villus distal CDX2 the intense of only CDX1, surface the to the contrast is on intestine expressed primarily the is product. as CDX2 gene interest either the cells. particular of levels in of epithelial detectable is contains involved intestinal that CDX1, organ be of as may well differentiation as and and CDX2, development proliferation intestinal of in regulation that early factor expressed transcription intestine-specific is homeobox, caudal-type a is CDX2 Use Diagnostic Vitro PA0535 In For ready-to-use Bond mL 7 AMT28 Clone CDX2 (HIER) P (HIER) P hnue spr fapnlo niois o h itnto of distinction the for antibodies, 95. page of to refer concentrate, panel Novocastra a as a available Also of part adenocarcinomas. most from as in mesotheliomas and, 5 carcinomas used Cytokeratin cell squamous of in when detection especially the tissues, neoplastic for and recommended normal non- is (XM26) many 5 Cytokeratin in proportions Information various Specific Product epitheliomas. in and tissue epithelia similar found basal a and is squamous shares stratified and keratinizing antigen 6 Cytokeratin The to related distribution. closely is 5 Cytokeratin Use Diagnostic Vitro PA0468 In For ready-to-use Bond mL 7 XM26 Clone 5 Cytokeratin rtr muoitceia tiigwt odrayt-s yoeai R7 sn Bond using (RN7) Detection. 7 Refine Cytokeratin Polymer ready-to-use Bond with staining immunohistochemical Ureter: the in 96. panel page to antibody refer concentrate, an Novocastra a of as available part Also as use carcinomas. of for of diagnosis recommended differential subtypes is RN7 specific Clone in Information expressed adenocarcinomas Specific Product whereas be negative. lung are tract and to gastrointestinal the breast from reported ovary from is adenocarcinoma 7 Cytokeratin Use Diagnostic Vitro PA0942 In For ready-to-use Bond mL 7 RN7 Clone 7 Cytokeratin Detection. Refine Polymer Bond ready-to-use using Bond (XM26) with 5 staining Cytokeratin immunohistochemical bowel: the of carcinoid Infiltrating W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence (HIER) P (HIER) P O E te applications Other lcrnmicroscopy Electron /25
Bond Bond o ealdifraino l rdcspes ii u website: our visit please www.leica-microsystems.com products all on information detailed For /26 Detection. Refine Polymer Bond using ready-to- (E3) Bond 17 with Cytokeratin staining use immunohistochemical esophagus: in carcinoma cell Squamous 97. page to refer concentrate, Novocastra a protein as available filament Also intermediate 17. cytokeratin Cytokeratin human as identified the kD) (46 with reacts E3 Clone in expressed Information cavity. be Specific oral the Product to and cervix reported sweat in lung, is the and of epithelium 17 glands carcinomas Cytokeratin cell salivary pseudostratified squamous tissue, in neoplastic of cells In myoepithelial cells glands. bronchi, of basal cells larynx, basal in eg trachea, expressed be epithelia to reported is complex 17 Cytokeratin tissues normal In Use Diagnostic Vitro In PA0114 For ready-to-use Bond mL 7 E3 Clone 17 Cytokeratin Detection. Refine Polymer Bond using (TS1) 8 Cytokeratin ready-to-use immunohistochemical Bond carcinoma): with ductal staining (infiltrating breast the of adenocarcinoma Papillary 96. page to also refer concentrate, is Novocastra a individuals, as available of Also adenocarcinomas sera. their the in present in 8, be Cytokeratin to expressed epithelia. found squamous be stratified components to of major not are and reported but 18, embryo epithelia and the simple 8 in all cytokeratins, expressed of Both cytokeratins tissues. first adult the in with of persists together one (TPA), is antigen 18, polypeptide tissue Cytokeratin as known also 8, Cytokeratin Use Diagnostic Vitro PA0567 In For ready-to-use Bond mL 7 TS1 Clone 8 Cytokeratin New! (HIER) P (HIER) P trsivsv acnm:imnhsohmclsann ihBn ed-oueCytokeratin ready-to-use Bond with staining immunohistochemical carcinoma: invasive Uterus 98. page to refer concentrate, Novocastra a as available Also of observed. staining layer in strong basal with pattern the follicles, staining hair heterogenous and epithelia complex squamous a non-keratinizing produces (b170) 19 Cytokeratin number Information large epithelia. Specific glandular a Product and in ductal expressed many be including to types, reported cell identified epithelial been been of has has and kD) 19 (40 Cytokeratin protein as filament cytokeratin human smallest The Use Diagnostic Vitro PA0799 In For ready-to-use Bond mL 7 b170 Clone 19 Cytokeratin oo:imnhsohmclsann ihBn ed-oueCtkrtn2 P3)using (PW31) 20 Cytokeratin ready-to-use Detection. Refine Bond Polymer with Bond staining immunohistochemical Colon: 98. page the to refer in concentrate, Novocastra panel a as available antibody Also an part as cells. use and Merkel epithelium intestinal for urothelium, the from originating recommended tumors in characterization is marker PW31 important Clone an Information therefore Specific Product is ovary and 20 carcinomas. lung of breast, diagnosis Cytokeratin differential to the reported from and negative. are carcinomas urothelium tissues are whilst these epithelium, 20 of almost intestinal Adenocarcinomas Cytokeratin is skin. express and It the expression. gastric of tissue cells the limited Merkel its to to confined due interest entirely of is 20 Cytokeratin Use Diagnostic Vitro PA0918 In For ready-to-use Bond mL 7 PW31 Clone 20 Cytokeratin Detection. Refine Polymer Bond using (b170) 19 hsctlgi o o s nteUSA. the in use for not approval. is regulatory catalog to This subject are catalog this in Products (Enzyme) P (HIER) P Hg oeua egt sn odPlmrRfn Detection. Refine Polymer Bond Cytokeratin using Weight) ready-to-use Molecular Bond (High with staining immunohistochemical carcinoma: cell Squamous 137. page to refer concentrate, Novocastra a as of available bowel. cells Also large and epithelia ductal small some and normal and the liver. acinar thyroid of normal normal some mesothelium of squamous and in pancreas, epithelia follicular ducts normal Expression: some bile the breast, bronchial normal and of 14. pneumocytes, lung cells some and ductal normal skin, Also in 10 normal mesothelium in and 5, ducts epithelium 1, sweat and proteins cytokeratin epithelium human filament with reacts 34ßE12 intermediate Weight) Molecular (High Cytokeratin Use Diagnostic Vitro In For 34 Clone Weight) Molecular (High Cytokeratin Detection. (5D3) Refine 8/18 Polymer Cytokeratin Bond ready-to-use using Bond with staining immunohistochemical mucosa: Colon 99. page to refer concentrate, Novocastra a as available Also negative. generally carcinomas, are carcinomas cell squamous squamous in keratinizing most panel but and antibody an adenocarcinomas of of part identification as use the for recommended cell is (5D3) 8/18 squamous Cytokeratin most been and Information have Specific Product adenocarcinomas they in tissues expressed neoplastic all carcinomas. in be In expressed to be epithelium. to shown reported glandular are 18 and and simple 8 Cytokeratins tissues, normal In Use Diagnostic Vitro PA0067 In For ready-to-use Bond mL 7 5D3 Clone 8/18 Cytokeratin LBn ed-ouePA0134 ready-to-use Bond mL 7 E12 (Enzyme) P (HIER) P scnie oteZbnsi ada n titdmsl iiga giving 101. muscle page to striated refer concentrate, Novocastra and a as available cardiac Also any with in react to bands appear proteins. not filament does Z other It pattern. the staining labeling striated The to characteristic cells. filament muscle intermediate confined smooth the and of striated is both region in rod kD) kD (53 18 desmin protein the with reacts DE-R-11 Clone Information Specific Product Use Diagnostic Vitro PA0032 In For ready-to-use Bond mL 7 DE-R-11 Clone Desmin Multi-Cytokeratin. about information further for 34 page to Refer Use Diagnostic Vitro In PA0909 For ready-to-use Bond mL 7 AE3 and AE1 Clone Multi Cytokeratin K)uigBn oye eieDetection. Refine Polymer DOG-1 Bond ready-to-use using Bond (K9) with staining immunohistochemical tumor: stromal Gastrointestinal 101. page to refer concentrate, Novocastra GISTs. a is negative as KIT be immunoreactivity available all Also often including GIST-1) GISTs, can scorable on that of percent Stromal tumors (Discovered 97.8 tissue in DOG-1 (Gastro-Intestinal reported soft GISTs. other GIST in with of expressed confused rarely membrane is plasma it Tumor), the on Expressed Use Diagnostic Vitro PA0219 In For ready-to-use Bond mL 7 K9 Clone DOG-1 Detection. Refine (DE-R-11) Polymer Desmin Bond using ready-to-use Bond with staining immunohistochemical Leiomyosarcoma: W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence (Enzyme) P (HIER) P (HIER) P O E te applications Other lcrnmicroscopy Electron /27
Bond Bond o ealdifraino l rdcspes ii u website: our visit please www.leica-microsystems.com products all on information detailed For /28 Detection. Refine Polymer Bond using (GP1.4) Epithelial Antigen ready-to-use Membrane Bond with staining immunohistochemical adenocarcinoma: Rectal 105. page epithelium to refer concentrate, squamous of Novocastra a while as cells available Also glands, lining expression. antigenic sweat in ductal of pattern reported eg uneven the been an shows has epithelia of is highest expression glandular EMA of the portion a pattern tissues, with other apical similar epithelia has normal A normal the In epithelium. of episialin, mammary kD. at variety 400 as a reported to in expression known expressed 265 be range also to the reported (EMA), in weight antigen molecular membrane Epithelial Use Diagnostic Vitro PA0035 In For ready-to-use Bond mL 7 GP1.4 Clone Antigen Membrane Epithelial Bond using Detection. (36B5) Refine E-Cadherin Polymer ready-to-use Bond with staining immunohistochemical Bowel: ductal and 102. page lobular to refer concentrate, in Novocastra a as protein available Also to this is of E-cadherin of expression carcinomas. utility differential immunohistochemical development also the highlight main has with is associated carcinomas metastases.The are which breast proteins of four primary prostate these in and addition or normal gamma-catenin reported in been and reduced in E-cadherin beta of be expression alpha, absence to or to its expression reported Reduced with is strong. uniformly E-cadherin comparison cancers, of in prostate expression In absent invasion. the of neoplastic example, in in suppressor role for step a a important as have an may particularly be E-cadherin progression, to invasion. local intercellular thought of is the development adhesiveness the and and of architecture abnormal development loss an subsequent growth, have any tumors the Most cells. in epithelial of role adhesion It important molecule. adhesion an cell transmembrane plays -dependent, Ca2+ a is E-cadherin Use Diagnostic Vitro PA0387 In For ready-to-use Bond mL 7 36B5 Clone E-Cadherin (HIER) P (HIER) P n aoal epnet norn hrp uha anti-estrogen as 106. page to such refer concentrate, Novocastra therapy a as available endocrine Also to response Tamoxifen. favorable and rsneo ER of Presence o ratcne.Coe61 id pcfclyt h srgnReceptor Estrogen the to specifically binds 6F11 prognosis (ER of alpha Clone prediction the cancer. in aid breast an for as indicated is (6F11) Receptor Estrogen Use Diagnostic Vitro In PA0151 For ready-to-use Bond mL 7 6F11 Clone Receptor Estrogen atezm eeae ntebodcauaincsae ti Ca a is It cascade. coagulation blood the the is in factor, fibrin-stabilizing generated and enzyme fibrinoligase as last known also XIIIa, Factor Use Diagnostic Vitro In PA0449 For ready-to-use Bond mL 7 E980.1 Clone XIIIa Factor Detection. Refine Polymer Receptor Bond Estrogen using ready-to-use (6F11) Bond with staining immunohistochemical carcinoma: Breast h yrglto fER of and dysregulation cells, The myometrial and ovarian cells. endometrial, ductal of breast normal proportion a ER; of levels loaalbea ooatacnetae ee opg 109. page to and upper refer concentrate, the Novocastra eczema in a as cells available atopic Also described. positive been XIIIa has eg lymphocytes, factor with associated dermatoses of closely number dermis, skin inflammatory increased benign an In as psoriasis, phagocytes. such mononuclear to which conditions common cells Langerhans features from some distinct population cells, and share specific dendritic a dermal represent marrow cells inflamed skin bone human and of in normal cells interstitial in positive expression XIIIa its Factor dendritic Factor determine skin. to dendrophages. examined positive been collagen-associated has elements. positive XIIIa CD34 XIIIa epithelial Factor of and scattered contains populations growth tissue stroma connective collagenous and mammary resident most like development of stroma, been mammary embryonic have proliferation Normal and proliferation, the tissues animal cell through in widespread with are associated centers active thiol with eitnet rtoyi.Fco II a lofnto osaiiecell stabilize to Ca function membranes. also its may and and XIIIa molecules clot fibrin Factor forms surface the proteolysis. of which to stabilization fibrin mechanical resistance the enzyme between in resulting crosslinks transamidating molecules gamma-glutamyl-epsilon-lysine or intermolecular transglutaminase dependent hsctlgi o o s nteUSA. the in use for not approval. is regulatory catalog to This subject are catalog this in Products nie ntence fclsi omltsusta xrs high express that tissues normal in cells of nuclei the in antigen ) nteetmr srltdt nipoe vrl survival overall improved an to related is tumors these in stogtt otiuet rattumorigenesis. breast to contribute to thought is (HIER) P (HIER) P 2+ dpnettrans-glutaminases -dependent 2+ - sn odPlmrRfn Detection. Refine (IM20) Polymer Fascin Bond ready-to-use using Bond with staining immunohistochemical lymphoma: Hodgkin’s the 110. with page to refer cells concentrate, Novocastra B a must as types available of cell Also other infection or cases. EBV-positive lymphoid in (EBV) in considered fascin be virus of also induction Epstein-Barr viral that may Reed by possibility expression Fascin all fascin. fascin. induced or for positive express most be be to cases, not reported are unclassified mixed cells do and Sternberg sclerosis, cells nodular depletion including lymphocyte plasma disease, cellularity Hodgkin's and of cases cells in cells. However, endothelial myeloid and cells cells, dendritic follicular Lymphoid subcapsular in seen in is reactive cells expression fascin. In Variable express zones, network cells. cell reticular the dendritic T of cells of medullary and areas, cells in reticulum only the interdigitating of in observed and nodes, detection cells been the dendritic binding to has tissues restricted actin predominantly thymus normal whose be In protein, to reported actin-bundling phosphorylation. is kD fascin by 58 regulated to is 55 ability a is fascin Human Use Diagnostic Vitro In PA0420 For ready-to-use Bond mL 7 IM20 Clone Fascin using Detection. (E980.1) Refine XIIIa Polymer Factor Bond ready-to-use Bond with staining immunohistochemical Placenta: (HIER) P loaalbea ooatacnetae ee opg 112. page to refer concentrate, Novocastra Hodgkin's a as of available variants Also or cells Sternberg whereas lymphomas, Reed cell in disease. large detected has anaplastic not Galectin-3 in is types. detected galectin-3 tumor to be various indicate to cells in may reported cancer phenotype and been enable metastasis invasive that and the events invasion of many facilitate activation to the laminin of with one interact be the could Downregulation as reported. galectin-3 breast expression cancer of of in correlation examined colon similar been a in also with carcinoma has downregulated basement downregulation is in This the progresses. involved and disease to be adhesion may binding galectin-3 cell including surface homotypical Cell events laminin. is glycoprotein biological It membrane family. several lectin beta-galactosidase-binding in the involved of member a is Galectin-3 Use Diagnostic Vitro In PA0238 For ready-to-use Bond mL 7 9C4 Clone Galectin-3 odPlmrRfn Detection. using Refine (Polyclonal) Polymer Gastrin Bond ready-to-use Bond with staining immunohistochemical Stomach: gastrin-analogue 113. page cholecystokinin to or refer concentrate, Novocastra with a gastrin as available Also cross-reacts labels antibody (polyclonal) cells. The producing Gastrin gastrin-34. octapeptide. as as gastrin-17 (II) sulfated and well (I) non-sulfated with reacts (polyclonal) Gastrin Information Specific cells Product immunoreactive glands. mucous the the hyperplasia of from cell clustering buds project by G sometimes characterized which secondary be and to primary gastrin-14, reported forms: are Both three gastrin-34. in and naturally occurs gastrin-17 hormone, polypeptide a Gastrin, Use Diagnostic Vitro In PA0681 For ready-to-use Bond mL 7 Polyclonal Gastrin Bond using Detection. (9C4) Refine Galectin-3 Polymer ready-to-use Bond with staining immunohistochemical Breast: W P F etr blotting Western Paraffin Frozen New! New! C I lwcytometry Flow Immunofluorescence (HIER) P P O E te applications Other lcrnmicroscopy Electron /29
Bond Bond o ealdifraino l rdcspes ii u website: our visit please www.leica-microsystems.com products all on information detailed For /30 Detection. using Refine (Polyclonal) Polymer Glucagon Bond ready-to-use Bond with staining immunohistochemical Pancreas: 114. page to refer concentrate, Novocastra found a as pancreas. are available the Also tumors of tail These or body glucagon. main the pancreatic from in arise produce chiefly to reported and are pancreas glucagonomas increased of the to majority lead The therefore, levels. and, sugar action blood insulin activities intestine. to the antagonistic biological large all of has These are and ketogenesis. effects acids, small cells and amino gluconeogenesis the 29 lipolysis, endocrine of glycogenolysis, of including mucosa the peptide the a in glucagon, in Pancreatic also reported and been islets pancreatic has expression Glucagon Use Diagnostic Vitro In PA0594 For ready-to-use Bond mL 7 Polyclonal Glucagon Acidic Fibrillary Glial ready-to-use Detection. Bond Refine Polymer Bond with using Protein staining immunohistochemical Astrocytes: 114. page to refer concentrate, sensory Novocastra a human as available of Also in cells expressed be to satellite reported ependymomas. been and and has astrocytomas GFAP cells tissues in neoplastic expressed in glial be and ganglia to enteric reported In been cells, cells. has ependymal Schwann GFAP and system, astrocytes nervous kD eg 52 peripheral cells of glial the protein in filament expressed intermediate be an to is reported (GFAP) protein acidic fibrillary Glial Use Diagnostic Vitro In PA0026 For ready-to-use Bond mL 7 GA5 Clone Protein Acidic Fibrillary Glial (HIER) P P pen muoitceia tiigwt odrayt-s rnyeB(11 sn Bond using (11F1) B Granzyme ready-to-use Bond with staining immunohistochemical Spleen: 116. page to refer concentrate, in Novocastra a reported as available also Also is Expression cells. NK to apoptosis. and channel and CTL pore-formed and neoplastic perforin perforin fragmentation a through DNA is containing cell induce B target Granzyme a granules activity. enter cytolytic to Secretory understood undertaking in neoplastic cells. cells. of instrumental elimination are (NK) infected the granzymes killer in natural involved virally heavily in are and and cells NK (CTL) and lymphocytes CTL These T lytic specialized cytotoxic in of stored are granules which proteases serine neutral are Granzymes Use Diagnostic Vitro In PA0291 For ready-to-use Bond mL 7 11F1 Clone B Granzyme ies li rti-5(33 sn odPlmrRfn Detection. Refine Polymer Bond using Cystic (23A3) Gross Protein-15 ready-to-use Fluid Bond Disease with staining immunohistochemical Disease): (Pagets Breast 116. page to of refer concentrate, Novocastra areas a as within available Also tumors. reported breast fibroadenomas receptor-positive are androgen antigen in in specific co-expressed breast. be prostate reported to normal and in been GCDFP-15 metaplasia. ducts also apocrine small and has positive lobules few Expression within a only cells the shows highest epithelial GCDFP-15 be individual of and to reported carcinomas is differentiated cells at concentration more GCDFP-15 The in contain concentrations. serous to of glands. range reported numerous wide are salivary a lesions minor in carcinoma breast and from all and sublingual Cytosol from bronchial, lacrimal, glands tissue tracheal, epithelia, normal Moll's apocrine submandibular, of in and analysis GCDFP-15 cytosolic demonstrated ceruminous that has reported organs GCDFP- been protein, major monomer has kD It 15 unique 15. composed to a is including appear fluid glycoproteins in This cysts several secretions. disorder These of cystic premenopausal apocrine lesion. excessive benign pathological from formed a predominant be a is are breast cysts the which of disease cystic Gross Use Diagnostic Vitro PA0350 In For ready-to-use Bond mL 7 23A3 Clone Protein-15 Fluid Disease Cystic Gross Detection. Refine Polymer hsctlgi o o s nteUSA. the in use for not approval. is regulatory catalog to This subject are catalog this in Products (HIER) P (HIER) P omn INhS-0 sn odPlmrRfn Detection. Refine Polymer Bond Stimulating using Follicle (INN-hFSH-60) Human Hormone ready-to-use Bond with staining immunohistochemical Pituitary: 2 FSH. beta human of the subunit recognizes beta the (INN-hFSH-60) of epitope Hormone Stimulating Follicle in Human secretion estrogen Information and Specific testosterone. Product of follicles is secretion which the ovarian stimulates kD FSH of 35 males, In of maturation females. hormone the pituitary in a is involved (FSH) hormone stimulating Follicle Use Diagnostic Vitro In PA0693 For ready-to-use Bond mL 7 INN-hFSH-60 Clone Hormone Stimulating Follicle Human Detection. Refine Polymer Bond using (Polyclonal) Chorionic Hormone Human Gonadotrophic ready-to-use Bond with staining immunohistochemical Placenta: the 121. of page to behavior refer intracellular concentrate, Novocastra the a as of in available region Also role C-terminal the a beta- that plays heterodimer. kD, believed of subunit is 29 17 It family HCG-beta of end. and extension the the COOH an its kD in contains at it acids 14 unique hormone- that amino in of is the hormones beta-subunit weights glycoprotein and containing hCG molecular chain The have alpha respectively. chains the common beta to follicle identical The specific and is hormone hormone. hCG stimulating of thyroid stimulating two subunit hormone, of alpha luteinising composed of The is subunit beta. hCG and endometrium. which alpha of secretory progesterone corpus subunits, produce the trimester the to maintains to first stimulated turn is binds in the which which ovary in hCG, the of of fetus luteum production hormone the days the 12 glycoprotein requires of to pregnancy 10 Maintenance a beginning placenta conception. is the after of cells (hCG) trophoblastic by gonadotrophin produced chorionic Human Use Diagnostic Vitro PA0014 In For ready-to-use Bond mL 7 Polyclonal Hormone Gonadotrophic Chorionic Human (Enzyme) P (HIER) P iutr:imnhsohmclsann ihBn ed-oueHmnGot Hormone Growth Human ready-to-use Bond with staining immunohistochemical Pituitary: 121. page to refer concentrate, Novocastra a hormone as stores. available growth fat Also general, up uses In and metabolic muscle. carbohydrates direct and conserves exerts synthesis, tissue act GH protein growth adipose enhances growth. which stimulates liver, cause the somatomedins, GH to on tissue of kD. effects soft production 22 and bone liver's of pituitary on weight the anterior directly molecular promoting the a by of has cells indirectly GH somatotropic Human or It metabolism. acidophilic gland. organic by in important for synthesized also responsible is is and hormone growth primary body the overall is regulating somatotropin, (GH), hormone Growth Use Diagnostic Vitro In PA0704 For ready-to-use Bond mL 7 Polyclonal Hormone Growth Human sn odPlmrRfn Detection. Refine (R1) Polymer Inhibin Bond ready-to-use Bond using with staining immunohistochemical tumor: cell theca Granulosa tumors. alpha stromal and cord placental The sex tumors, and adrenocortical genes. lesions of trophoblastic differentiation separate gestational the antibody in The by value produced. of be coded be will may activin are 2 or has inhibin that whether Inhibin determines beta) gland. subunit pituitary and the (alpha by activate, follitropin subunits and of inhibit activins secretion and to the female inhibins appear respectively, as They in activins, tubules. of cells seminiferous functions male granulosa the the oppose in the cells by Sertoli by produced and follicles hormones peptide are Inhibins Use Diagnostic Vitro In PA0110 For ready-to-use Bond mL 7 R1 Clone (alpha) Inhibin Detection. Refine Polymer Bond using (Polyclonal) W P F etr blotting Western Paraffin Frozen New! C I lwcytometry Flow Immunofluorescence (HIER) P P O E te applications Other lcrnmicroscopy Electron /31
Bond Bond o ealdifraino l rdcspes ii u website: our visit please www.leica-microsystems.com products all on information detailed For /32 Detection. Refine Polymer Ki67 Bond ready-to-use using Bond with (K2) staining immunohistochemical carcinoma: ductal invasive Breast cell of 126. page to assessment refer concentrate, the Novocastra a as for available Also recommended tissues. neoplastic is and normal in Ki67 proliferation ready-to-use Bond Information cycle. Specific division Product cell role the important of an associated regulation suggesting DNA and/or strictly nucleus, maintenance the during is the to antigen in detectable confined and Ki67 not cycle of cell In is is the presence (G0). with antigen the and cells Ki67 Thus, cells resting processes. the in quiescent repair proteins, absent in is cycle-associated absent but cell mitosis) consistently other and of many parts G2 to active S, all contrast in (G1, expressed cycle is which cell protein the nuclear a is antigen Ki67 The Use Diagnostic Vitro In PA0230 For ready-to-use Bond mL 7 K2 Clone PA0118 ready-to-use Bond mL 7 MM1 Clone Ki67 Detection. Bond Refine using (2D11-H5) Polymer Insulin ready-to-use Bond with staining immunohistochemical Pancreas: 125. page to refer concentrate, Novocastra a sparse as available are Also membrane. cell granules the insulin-positive against islet margin instances certain a immunocytochemical form of some and of cytoplasm in the Reports in However, insulin acids. tumors. of presence nucleic the and and reveal triglycerides, investigation in protein of Langerhans formation of of storage, islets the glycogen synthesis of promotes cells It beta pancreas. the by the secreted hormone a is Insulin Use Diagnostic Vitro In PA0620 For ready-to-use Bond mL 7 2D11-H5 Clone Insulin New! (HIER) P (HIER) P P sn odPlmrRfn Detection. Refine (C93) Polymer Hormone Bond Luteinizing using ready-to-use Bond with staining immunohistochemical Pituitary: characterizes which hormones. beta-subunit these composed specific human of a is each the like and hormone, alpha-subunit hormone, stimulating common modulates a glycoprotein thyroid anterior of and which the This hormone by gland. stimulating hormone produced pituitary follicle is trophic the It glands. of a endocrine hypophysis other is of activity (LH) secretory hormone Luteinising Use Diagnostic Vitro In PA0655 For ready-to-use Bond mL 7 C93 Clone Hormone Luteinizing sn odPlmrRfn Detection. Refine (MAC387) Polymer Marker Bond Macrophage using ready-to-use Bond with staining immunohistochemical Lung: L1. antigen leucocyte the for specific be to reported is MAC387 on Clone found squamous be Information and to Specific Product reported macrophages is proteins, reactive of epithelia. certain family mucosal S-100 monocytes, the of neutrophils, member a L1, Use Diagnostic Vitro In PA0752 For ready-to-use Bond mL 7 MAC387 Clone Marker Macrophage hsctlgi o o s nteUSA. the in use for not approval. is regulatory catalog to This subject are catalog this in Products New! (HIER) P P sn odPlmrRfn Detection. (A013) Refine A Polymer Melan Bond ready-to-use using Bond with staining immunohistochemical melanoma: Malignant 132. page to refer concentrate, melanocytic Novocastra in a as A available Melan Also of assessment the in employed lesions. is (A103) A Melan differentiation melanocytic a Information is Specific Product gene, lymphocytes. MART-1 T cytotoxic the autologous by of recognized product marker a A, Melan Use Diagnostic Vitro PA0233 In For ready-to-use Bond mL 7 A103 Clone A Melan Detection. (10D11) Refine Tryptase Polymer Cell Bond Mast using ready-to-use Bond with staining immunohistochemical Bowel: of 130. page to mediator refer for concentrate, Novocastra a responsible a as disease. be available as bowel Also inflammatory may of implicated signs and secretion, and symptoms permeability mucus be the causes of gut some gut to increased the edema, reported in mast mucosal degranulation Human been C3a cell levels. Mast skin, tryptase has form inflammation. elevated the to indicated in tryptase each disease C3 cell have allergenic lung of cleave and Models thrombin can nose collagenase. for substrate latent tryptase coaguable activate a indicate and mediated A as cell fibrinogen studies mast in inactivate skin role tryptase. vitro of anaphylatoxin, major cells a mast In plays in proteolytic enzyme found events. as this be suggests such to and and reported lung mediators is and chemical tryptase carboxypeptidase of preformed quantity substantial of chymase, number a histamine, contain cells Mast Use Diagnostic Vitro In PA0019 For ready-to-use Bond mL 7 10D11 Clone Tryptase Cell Mast (HIER) P P loaalbea ooatacnetae ee opg 133. page to refer concentrate, Novocastra a as available Also other from to cystadenocarcinomas antibodies of ovarian tumors. panel solid a and of part mesotheliomas as use distinguish for recommended is (5B2) Mesothelin ovarian and tumors. mesotheliomas solid distinguish other to for are antibodies from recommended of cystadenocarcinomas panel is cystadenocarcinomas a (5B2) of part Mesothelin ovarian as ready-to-use use from Bond and cells such, mesothelial As mesothelioma normal derived. the malignant in abundant be which to reported is Mesothelin Information unclear Specific adhesion. Product is cellular mesothelin of in role of cells a function epithelial have The may occasional tube. it in but fallopian and and tonsil kidney trachea, mesothelioma, the the in cells, Mesothelin expressed mesothelial carcinomas. abundantly cell of squamous is some surface and cancers the ovarian on epithelial present is Mesothelin Use Diagnostic Vitro PA0373 In For ready-to-use Bond mL 7 5B2 Clone Mesothelin loaalbea ooatacnetae ee opg 134. page to refer concentrate, Novocastra eg a as available cancer Also gastric sporadic colon been carcinoma, also of endometrial of carcinoma. has ovarian leukemia, MLH1 and subset forms carcinoma lymphoblastic of acute expression a human some of in (HNPCC), Loss as to be reported cancer. to cancer such breast linked and reported colon MSI, carcinomas been been with nonpolyposis have have tumors gene Hereditary pathways MLH1 in to repair the found alterations in DNA (MSI). DNA Mutations in instability in carcinogenesis. microsatellite During resulting as defects PMS2. to occur These referred and of often can integrity repeats, MSH6 misalignment the microsatellite MSH2, maintaining strand in alongside replication, involved information, protein repair genetic mismatch a MLH1, Use Diagnostic Vitro In PA0610 For ready-to-use Bond mL 7 ES05 Clone Protein) Repair (Mismatch MLH1 Detection. using Refine (5B2) Polymer Mesothelin Bond ready-to-use Bond with staining immunohistochemical Mesothelioma: W P F etr blotting Western Paraffin Frozen New! C I lwcytometry Flow Immunofluorescence (HIER) P (HIER) P O E te applications Other lcrnmicroscopy Electron /33
Bond Bond o ealdifraino l rdcspes ii u website: our visit please www.leica-microsystems.com products all on information detailed For /34 Bond using Detection. (25D12) Refine MSH2 Polymer ready-to-use Bond with staining immunohistochemical Bowel: 134 before page continuous to days refer concentrate, 4 the Novocastra a to as for available 2 Also over responsible migrate are lumen. the which which are into sloughed of cells being cells They differentiated the of renewal. crypts, colon, smooth production and the heart, ileum rapid in thyroid, In reported including follicles. undergoing been multistage lymphoid tissues has of the expression of centers in which MSH2 variety germinal for expressed the a cancers be and from to sporadic muscle required cells reported to of is to MSH2 and nuclei be mutations linked instability. may cancer been microsatellite turn have colon exhibit multiple genes in nonpolyposis repair which mismatch hereditary the errors, in function Mutations replication MSH2 carcinogenesis. for of of loss accumulation The responsible the process. to repair during mismatch leads nucleotides mismatched replication of post recognition initial the the in involved is MSH2 Use Diagnostic Vitro In PA0048 For ready-to-use Bond mL 7 25D12 Clone Protein) Repair (Mismatch MSH2 Bond using Detection. (ES05) Refine MLH1 Polymer ready-to-use Bond with staining immunohistochemical Appendix: New! (HIER) P ntblt.MH a enrpre ob sflmre ouein use 135. page to to refer concentrate, marker Novocastra a useful as available HNPCC. Also a as such mutations, be tumors gene MMR colon to contain identify may to that reported screening instability been microsatellite with microsatellite in conjunction has with lost carcinomas MSH6 be colonic colonic instability. can and carcinomas normal expression and endometrial lymphocytes. sometimes in carcinomas some gastric in However, in cells expressed found carcinomas. is of also MSH6 endometrial that is nucleus shown also Expression the that have crypts. Studies in reported in expressed especially have epithelium, strongly studies is Immunohistochemical DNA MSH MSH6 disrupted. other with cause is along expression, proteins, can MSH6 where Nonpolyposis (HNPCC) and Hereditary Cancer in Colorectal particularly has mutations cancer, instability colorectal in Microsatellite cause described unstable. been become can to sequences (MMR) systems microsatellite in repair Defects mismatch repair MSH2. DNA with in mismatch heterodimerized involved when is pathways, which recombination protein and kD 160 a is MSH6 Use Diagnostic Vitro In For PA0597 ready-to-use mL 7 PU29 Clone Protein) Repair (Mismatch MSH6 loaalbea ooatacnetae ee opg 136. page to refer concentrate, Novocastra a as available acidic Also families kD two 40 with reactivity basic. and and broad cytokeratins. basic 48 acidic exhibit kD cytokeratin, AE3 52 50', of and and AE1 50, 56 58, clones 56.5, 59, of 64, cocktail the 67, The to for 65 the specific as well are as cytokeratins AE3 and AE1 Clones Information and Specific Product differentiation be keratinization. for to terminal markers reported molecular undergoing are stratum as considered cytokeratins during cells be kD may eliminated 67 epidermal normally to of 65 is entire and characteristic and to 56 the reported layer The is throughout basal formation. cytokeratin expressed corneum the kD 56 from be the absent to and layer, be layer reported basal basal the is the layer above including cytokeratin only epidermis 65 basal present kD The be bio- layer. germinative to 58 cornified reported and the outer the are epidermal the cytokeratins morphological kD to from normal 67 layers of to granular of progress and series process spinous cells the These The a through as kD. epithelia. by changes 70 other characterized chemical to all 40 is almost of differentiation as in proteins epidermis in as insoluble filament, intermediate well water of class of a tonofilaments, family form proteins a are Keratins Use Diagnostic Vitro In PA0909 For ready-to-use Bond mL 7 AE3 and AE1 Clone Cytokeratin Multi Refine. Polymer pedx muoitceia tiigwt odrayt-s L6(U9 sn Bond using (PU29) MLH6 ready-to-use Bond with staining immunohistochemical Appendix: hsctlgi o o s nteUSA. the in use for not approval. is regulatory catalog to This subject are catalog this in Products New! (HIER) P (Enzyme) P ylm noee1(U1 EU2 sn odPlmrRfn Detection. Refine Polymer Bond using (EAU32) (MUM1) 1 Multiple Oncogene ready-to-use Myeloma Bond with staining immunohistochemical lymphoma: Hodgkin's 136. page neoplastic to refer and concentrate, Novocastra a normal Reed as available and cells, Also Disease, myeloma Hodgkin in a classic cells. MUM-1 T expressed including in neoplasms consistently cells). cells hemopoietic extra lymphoid Sternberg is or plasma myeloid of not MUM-1 and but range lymphomas neoplasm's. cell (immunoblasts wide B diffuse a positive of cell. proportion in plasmablast/plasma CD138 occurs a center to expression (germinal positive of to BCL-6 cells) marker from that a transition provide B the to cell of to identification be the centrocyte may in to B MUM-1 aid of a found value been of histogenic of the has cells. Consequently that cells stages these expressing from from MUM-1 directed derived range of neoplasms spectrum serve cell may lympho-hemopoietic morphologic MUM-1 for The that is plasma marker suggests protein and a the cells late as T that activated indicates in in and MUM-1 differentiation to expressed antibody the An to strongly locus. gene MUM-1 chain the in heavy of observed juxtaposition Ig translocation the t(6:14) causes which the myeloma, in multiple involvement its of originally because was identified gene 1)/FR4/ACSAT/PiP Oncogene Myeloma (Multiple MUM-1 Use Diagnostic Vitro In PA0129 For ready-to-use Bond mL 7 EAU32 Clone (MUM-1) 1 Oncogene Myeloma Multiple Detection. Refine Polymer Bond Multi- using ready-to-use (AE1/AE3) Bond Cytokeratin with staining immunohistochemical carcinoma: cell Squamous New! (HIER) P plcoa)uigBn oye eieDetection. Refine Polymer Muramidase Bond ready-to-use using Bond (polyclonal) with staining immunohistochemical leukemia: myeloid Acute 129. page to in refer concentrate, expressed Novocastra myelo- a be as in available to Also and reported leukocytes also monoblasts. in leukemic is differentiated cells, poorly Muramidase monocytic disorders. be and to proliferative myeloid reported been in has lysozyme, expressed as known also muramidase, Intracellular Use Diagnostic Vitro In PA0391 For ready-to-use Bond mL 7 Polyclonal (Lysozyme) Muramidase ci HF5 sn odPlmrRfn Detection. Refine Polymer Bond using Specific (HHF35) Muscle Actin ready-to-use Bond with staining immunohistochemical Leiomyosarcoma: 138. page to refer concentrate, Novocastra a as available Also and wall. stroma bladder prostatic muscle, the of smooth of myometrial fibers coat tract, muscle muscle gastrointestinal smooth striated entire wall, in cell of arterial expressed muscle, gamma-actins be skeletal and myocardium, to the alpha- reported kD) for (42 specific muscle is smooth (PA0258) Actin Specific Muscle Information cells. Specific muscle Product in present and is cells gamma-actins non-muscle of in subset and present minor are a biochemically cyto- gamma-actins in be and present are beta- ubiquitous can alpha-actins tissue, subsets; muscle Actins the main cells. three microfilaments, into most divided immunologically in of present constituents elements skeletal protein are Actins Use Diagnostic Vitro PA0258 In For ready-to-use Bond mL 7 HHF35 Clone Actin Specific Muscle W P F etr blotting Western Paraffin Frozen New! C I lwcytometry Flow Immunofluorescence (Enzyme) P P O E te applications Other lcrnmicroscopy Electron /35
Bond Bond o ealdifraino l rdcspes ii u website: our visit please www.leica-microsystems.com products all on information detailed For /36 Detection. Refine Polymer Bond using (LO26) Marker) Myf-4 (Rhabdomyosarcoma ready-to-use Bond with staining immunohistochemical Rhabdomyosarcoma: 139. page to tumors. refer cell has concentrate, blue Myf-4 Novocastra round a small cells. as of available differentiated typing Also sub with of the in together nucleus useful Myf-6. product, the be to gene and in shown Its been Myf-5 accumulates myogenin. The, myogenin, of of MyoD1, homolog that human including the genes by is regulatory controlled Myf-4 is of cells myogenic set of affect a Differentiation primarily and adults. genes young muscle-specific and of sarcomas children number tissue a soft express myoblast-derived usually of that class a are Rhabdomyosarcomas Use Diagnostic Vitro PA0226 In For ready-to-use Bond mL 7 LO26 Clone Marker) (Rhabdomyosarcoma Myf-4 Detection. using Refine (59A5) Polymer Myeloperoxidase Bond with staining immunohistochemical leukemia: myeloid Acute 139. page to refer concentrate, Novocastra a as the available liver. in Also the granulocytes of myelocyte cells of the Kupffer precursors in from neutrophil and in marrow in detectable blood, bone expressed in not be monocytes is to and reported and granulocytes is cell Myeloperoxidase the onwards. decreases granulocytes of stage level mature whose maturation including mRNA, the myeloperoxidase cells, to with myeloid marker superior all a reactsin is and component to HOCI reported major neutrophils. is a Myeloperoxidase by chloramines. be cytotoxic produced form be to most proteins the to with is known HOCI oxidant. oxidant strong granules a and bacteriocidal (HOCI), compounds cytoplasmic acid aromatic hypochlorous of azurophilic to variety ions a of chloride oxidise activated to constituent peroxide by major hydrogen uses generated a that peroxide is hydrogen series It myeloid the the phagocytes. of of most cells in metabolises found which enzyme lysosomal a is Myeloperoxidase Use Diagnostic Vitro In PA0491 For ready-to-use Bond mL 7 59A5 Clone Myeloperoxidase New! (HIER) P P MO8 sn odPlmrRfn Detection. Refine Polymer Bond using Myoglobin (MYO18) ready-to-use Bond with staining immunohistochemical Rhabdomyosarcoma: 139. page to refer concentrate, Novocastra an a as in as functions available expressed Also and be muscle smooth to in reported pigment. not transporting is but oxygen muscle Myoglobin cardiac group. and that heme skeletal acids amino single 153 of a polypeptide contains chain single cytoplasmic, a is Myoglobin Use Diagnostic Vitro In PA0727 For ready-to-use Bond mL 7 MYO18 Clone Myoglobin hi sot uce S3)uigBn oye eieDetection. Refine Polymer Bond using (S131) muscle) Heavy (smooth Myosin ready-to-use Chain Bond with staining immunohistochemical muscle smooth Prostate: 140. page to refer concentrate, Novocastra a development. as muscle structural available smooth Also for cytoplasmic specific muscle be smooth a to in reported apparatus is been contractile has the It (SM-MHC) of cells. component major chain a regulated. is heavy developmentally that are protein for myosin which specific of muscle are some which types, Smooth isoforms fiber many or has four muscles chain and different heavy heavy two myosin of The composed chains. protein light specific muscle contractile a is Myosin Use Diagnostic Vitro PA0493 In For ready-to-use Bond mL 7 S131 Clone Muscle) (Smooth Chain Heavy Myosin hsctlgi o o s nteUSA. the in use for not approval. is regulatory catalog to This subject are catalog this in Products (HIER) P (HIER) P osl muoitceia tiigwt odrayt-s eaie(abt sn Bond using Detection. (Rabbit) Refine Negative Polymer ready-to-use Bond with staining immunohistochemical Tonsil: the at staining specific of interpretation better rabbit site. of allow bind antigenic identification non-specifically will the may in and which aid components, antibodies to tissue recommended or is tissues (Rabbit) cells, Negative of use The Information Specific Product or neoplastic in especially occur, tissue. may necrotic binding non-specific tissues, some In Use Diagnostic Vitro In PA0777 For ready-to-use Bond mL 7 (Rabbit) Control Negative Bond using Detection. (Mouse) Refine Negative Polymer ready-to-use Bond with staining immunohistochemical Tonsil: non- of the may interpretation in which better aid allow components, site. will to antigenic the tissue and at recommended staining antibodies or specific is mouse tissues antibody bind cells, specifically (Mouse) of Negative identification of use The Information Specific Product or neoplastic in especially occur, tissue. may necrotic binding non-specific tissues, some In Use Diagnostic Vitro In PA0996 For ready-to-use Bond mL 7 MOPC-21 Clone (Mouse) Control Negative New! P P New! sn odPlmrRfn Detection. Refine Polymer (N52.1.7) Bond Neurofilament using ready-to-use Bond with staining immunohistochemical Cerebrum: 142. page to refer concentrate, Novocastra a as available Also neurofilaments. exhibiting express in those to or reported present been origin have neural be differentiation, of ganglion neuronal cells to neoplastic sympathetic kD, reported only and 68 tumors, are subunits Within nerves of cells. subunits peripheral weights major processes, Neurofilament molecular neuronal kD. three with neurons, 200 triplet, of and neurofilament kD composed the 160 as are axons to neuronal Neurofilaments referred of elements dendrites. structural and main the constitute Neurofilaments Use Diagnostic Vitro In PA0371 For ready-to-use Bond mL 7 N52.1.7 Clone 200kD Neurofilament 2C)uigBn oye eieDetection. Refine Polymer Enolase Bond Specific using Neuron (22C9) ready-to-use Bond with staining immunohistochemical Carcinoid: the with reacts 22C9 Clone Information Specific Product The respectively. kD, 45 and kD 47 approximately of are ( subunit subunits distinct three of enolase composed mammals, dimers In are molecules pyruvate. between phosphoenol pathway reaction and the catalyzing 2-phospho-glycerate enzyme glycolytic a is Enolase Use Diagnostic Vitro In PA0435 For ready-to-use Bond mL 7 22C9 Clone Enolase Specific Neuron loaalbea ooatacnetae ee opg 142. page of to refer panel concentrate, Novocastra a a as neuronal of available of Also part cells neoplastic as and use normal origin. neuroendocrine of for and identification recommended the for is antibodies (22C9) Enolase Specific eredciecells. neuroendocrine W P F hra,i as iefrshv enfud The found. been have forms five rats, in whereas, nlssaelctdmil ntenrostsu and tissue nervous the in mainly located are enolases etr blotting Western Paraffin Frozen New! 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Bond Bond o ealdifraino l rdcspes ii u website: our visit please www.leica-microsystems.com products all on information detailed For /38 Bond using Detection. (N1NK) Refine Oct-3/4 Polymer ready-to-use Bond with staining immunohistochemical Seminoma: some for 143. origin page to refer cell concentrate, Novocastra a and germ exhibit as available Also which a seminoma/dysgerminoma tumor. tumors primary establishing uncertain cell of including tumors and germ metastatic for pluripotentiality, carcinoma, critical marker A embryonal of useful cells. has a germ Oct3/4 features pluripotency. and as of loss cells proposed with associated stem been are levels embryonic Oct3/4 factors, by of transcription amount of expressed family homeodomain is POU the which of member a is Oct3/4 Use Diagnostic Vitro In PA0934 For ready-to-use Bond mL 7 N1NK Clone 3/4 Oct using Detection. (Oct-207) Oct-2 Refine ready-to-use Polymer Bond Bond with staining immunohistochemical lymphoma: cell B 143. page to refer concentrate, Novocastra a expression. as of available levels lymphomas, although Also high is derived display expression also center cells, lymphomas germinal Oct-2 cell cells in B B BOB-1/OBF-1. other greater B although and significantly to center be Oct-2 Germinal to restricted cells. for reported these expression not in higher higher is show much expression are levels RS protein expression in expression transactivating deregulation BOB-1 Oct-2 gene and the immunoglobulin Oct-2 cells. for and both mechanism of novel sites absence a the represents octamer BOB.1, and also between Oct-2 cells B factors interaction in of expression presence gene an Ig the As to requires genes. due Ig immunoglobulin the within express number mutations not a crippling do In cells. cells classical B cases, in center these germinal BOB-1/ cells of from as malignant derived are such the and represent coactivators disease is cells Hodgkin's restricted It (RS) cell genes. Sternberg specific B Reed cell of family B OBF-1. activity regulating homeo-domain the sites POU octamer on the gene dependent Ig to the belonging to factor binds that transcription a is Oct-2 Use Diagnostic Vitro In PA0532 For ready-to-use Bond mL 7 Oct-207 Clone Oct-2 New! (HIER) P (HIER) P loaalbea ooatacnetae ee opg 145. page to refer concentrate, Novocastra for a as recommended and available Also breast is including carcinomas, (DO7) of variety p53 of a carcinomas. forms of colorectal ready-to-use status mutant p53 Bond and the type determining protein. wild p53 both recognizes human antibody the monoclonal This by apoptosis well is and Information Specific vitro arrest Product in growth damage genes. induce other DNA of to regulation to transcriptional appears response The cancer. in and of protein development established the p53 suppressing of in role accumulation vital a plays protein p53 Use Diagnostic Vitro PA0057 In For ready-to-use Bond mL 7 DO-7 Clone Protein p53 sn odPlmrRfn Detection. Refine Polymer Bond (7JUL) p63 using ready-to-use Bond with staining immunohistochemical carcinoma: Nasopharyngeal differentiated poorly 72. most page to refer in concentrate, Novocastra a expressed as available be Also to and urothelium reported carcinomas. cervix, cell is squamous epithelium, normal the p63 of of number a prostate. cells in proliferating expressed rough be the including to in tissues reported localized predominantly is protein p63 membrane reticulum. integral endoplasmic II type a is p63 Use Diagnostic Vitro PA0478 In For ready-to-use Bond mL 7 7JUL Clone p63 Detection. (DO-7) Refine Protein Polymer p53 Bond ready-to-use using Bond with staining immunohistochemical carcinoma: Bladder hsctlgi o o s nteUSA. the in use for not approval. is regulatory catalog to This subject are catalog this in Products (HIER) P (HIER) P hshts 89 sn odPlmrRfn Detection. Refine Polymer Bond using Alkaline (8A9) Placental Phosphatase ready-to-use Bond with staining immunohistochemical Seminoma: 150. page to refer concentrate, pre- Novocastra in a described as be available recently Also to more percent seminomas. of and detection reported 85 tumors the also in is cell is Used It than seminomas. thymus. germ in enzyme in more and testis, expressed neonatal PLAP-like shares commonly and fetal itself. which normal in found described PLAP dominantly PLAP- been with A is from: molecule has homology expression distinct a PLAP variant is be This tumors. tumors. like to cell gastrointestinal germ and reported proximal malignant tube in is and fallopian rare and PLAP ovarian endocervix gestation. in placenta, term also of enzyme normal the trimester in within sialoglycoprotein only third syncytiotrophoblasts expressed the in membrane-associated during concentration phos- high placenta a alkaline at Placental present is enzyme. normally PLAP-like (PLAP) and a PLAP phatase indicating placenta both and for seminomas stains specificity 8A9 Clone that indicate Reports Use Diagnostic Vitro PA0161 In For ready-to-use Bond mL 7 8A9 Clone Phosphatase Alkaline Placental Bond using Detection. (1EW) Refine Pax-5 Polymer ready-to-use Bond with staining immunohistochemical Lymphoma: cell B of stages 148. page all to refer concentrate, in Novocastra a downregulated. subsequently as is available it Also and which in stage cells cell plasma B the until development mature pre-B, cells, i PAX-5 mammalian of control the nuclear in encode implicated development. that been genes have control and developmental factors of transcription family a are genes Pax Use Diagnostic Vitro In PA0552 For ready-to-use Bond mL 7 1EW Clone Pax-5 elseii rncito atr hti xrse npoB pro in expressed is that factor, transcription specific cell B a s (HIER) P (HIER) P ratcrioa muoitceia tiigwt odrayt-s Progesterone ready-to-use Bond with staining immunohistochemical carcinoma: Breast 152. page to refer concentrate, Novocastra a as available Also formalin-fixed, in receptor progesterone of form A tissues. the paraffin-embedded to of specific is prediction 16 Clone and prognosis Information management, Specific Product this in aid an outcome. therapy is Progesterone therapy. (16) endocrine to in response Receptor parameter and important prognosis an of, is prediction cancer the breast in content Receptor Progesterone Use Diagnostic Vitro PA0312 In For ready-to-use Bond mL 7 16 Clone Receptor Progesterone 3H)uigBn oye eieDetection. Refine Polymer Antigen Bond Specific using Prostate (35H9) ready-to-use Bond with staining immunohistochemical Prostate: 153. page to refer concentrate, Novocastra a as protein available Also from of isolated be protein family to a found gland. kallikrein to been prostate similar the the has biologically it of and Phosphatase, identical protein Acid immunologically Prostatic a from Distinct is kinases. antigen specific Prostate Use Diagnostic Vitro In PA0431 For ready-to-use Bond mL 7 35H9 Clone Antigen Specific Prostate Detection. Refine Polymer Bond using (16) Receptor W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence (HIER) P (HIER) P O E te applications Other lcrnmicroscopy Electron /39
Bond Bond o ealdifraino l rdcspes ii u website: our visit please www.leica-microsystems.com products all on information detailed For /40 Detection. Refine Polymer Bond Gene using Protein (10A1) ready-to-use 9.5 Bond Product with staining immunohistochemical fibers: nerve bowel Small 154. page to refer concentrate, Novocastra a as available Also cells. the of and structures regions such family active metabolically that hydroxylase be suggesting C-terminal may bodies ubiquitin inclusion the within PGP9.5 of concentrated luteum. also member is corpus a and non-pregnant be ova and to in pregnant known testis, the is of the both of of segments cells cells Leydig in some and in cells, spermatogonia and in neuroendocrine central tubules, the many renal of the in levels all system, at fibers nervous expression nerve peripheral and PGP9.5 neurons enolase. in and reported specific structurally been protein, has neuron specific from neuron a distinct is immunologically 9.5 (PGP) product gene Protein Use Diagnostic Vitro In PA0286 For ready-to-use Bond mL 7 10A1 Clone 9.5 Product Gene Protein Detection. Refine Polymer Bond using (PASE/4LJ) Prostatic Phosphatase ready-to-use Acid Bond with staining immunohistochemical adenocarcinoma: Prostate 154. page to refer concentrate, Novocastra a precise as levels available carcinoma. serum The Also prostatic Elevated metastatic fluid. transferase. in reported phosphoryl a seminal are split as enzyme to function the hydrolase and also of a as and prostate phosphatase act semen may acid in the it choline but of in unknown, is isoenzyme PAP amounts of an function large is in (PAP) found phosphatase acid Prostatic Use Diagnostic Vitro In PA0006 For ready-to-use Bond mL 7 PASE/4LJ Clone Phosphatase Acid Prostatic (HIER) P P ugmtsai eaoa muoitceia tiigwt odrayt-s S-100 ready-to-use Bond with staining immunohistochemical melanoma: metastatic Lung 157. page to refer concentrate, the Novocastra a for as antibodies available Also of panel origin. a neuroectodermal in of use tumors of for determination recommended is (polyclonal) S-100 interdigitating and Information Specific express skin Product to reported also in are nodes cells lymph protein. of S-100 paracortex Langerhans’ the in cells melanocytes. reticulum including tissue, and neuroectodermal in expressed nerves be to reported is protein S-100 Use Diagnostic Vitro In PA0900 For ready-to-use Bond mL 7 Polyclonal S-100 odPlmrRfn Detection. Refine using Polymer (Polyclonal) Bond Serotonin ready-to-use Bond with staining immunohistochemical Carcinoid: lungs cells), 158. page to enterochromaffin refer concentrate, Novocastra gastro- a as plexus, platelets, available Also as spleen. (myenteric and such gland be thyroid structures cells), to (neuroepithelial tract reported non-neural is and serotonin an periphery, intestinal neural and be the motor in In to to sensory, CNS. thought present of project the is in range Serotonin which functions wide cord. in Raph brainstem cortical a spinal nucleus originate regulating the and neurotransmitter brain median), in CNS inhibitory the (dorsal, pallidus of the nuclei areas Raph in Raph specific nucleus the terminals and of nerve obscurus bodies serotonergic cell by of neuronal schizophrenia. and majority formed depression anxiety, The several as terminal in is implicated such presynaptic been disorders the the has neuropsychiatric into Serotonin turn CNS, reuptake transporters. serotonin the by specific terminated in In by is 5- hydroxylase. serotonin tryptophan of which of by decarboxylation action L-tryptophan the intermediate, of by central hydroxylation formed and its is peripheral Serotonin mammalian hydroxy-tryptophan, (CNS). the system in distributed nervous widely hormone a be and to neurotransmitter reported is 5-HT) (5-hydroxytryptamine, Serotonin Use Diagnostic Vitro In PA0736 For ready-to-use Bond mL 7 Polyclonal Serotonin Detection. Refine Polymer Bond using (Polyclonal) hsctlgi o o s nteUSA. the in use for not approval. is regulatory catalog to This subject are catalog this in Products (Enzyme) P (HIER) P LBn ed-ouePA0943 ready-to-use Bond mL 7 sn odPlmrRfn Detection. Refine Polymer (Polyclonal) Bond Somatostatin using ready-to-use Bond with staining immunohistochemical Pancreas: pancreas region. mammalian parvicellular the hypothalamic of the cells of cells D 80 and for to specific is 40 (polyclonal) Somatostatin contain intestine the Information Specific of the Product rest of the cells and and somatostatin-28. body duodenum endocrine percent gastric antrum, in somatostatin-14, exclusively the The and almost whereas contain islets. to wall reported pancreatic been intestinal have the pancreas the and identified mucosa of been has digestive nerves somatostatin system, intrinsic somatostatin-14, digestive the composed forms, in In prohormone two acids. a amino in somatostatin-28, growth 28 and exists of of acids It amino release 14 gland. inhibit of the pituitary composed to from the ability isolated from its originally hormone by hormone characterized polypeptide and cyclic hypothalamus a is Somatostatin Use Diagnostic Vitro In PA0331 For ready-to-use Bond mL 7 Polyclonal Somatostatin Smooth ( ready-to-use Actin Bond Muscle with staining immunohistochemical muscle: smooth Appendix, 57. page to refer concentrate, Novocastra a as available Also leiomyomas as such tissues the neoplastic notably leiomyosarcomas. in most and and glands have glands various They of mammary tissues. cells and many myoepithelial salivary muscularis the of in intestinal stroma noted the walls, been in also vascular and the propria in muscularis located mucosae, be can muscle Smooth Use Diagnostic Vitro In For Clone Actin Muscle Smooth sm-1 m1 sn odPlmrRfn Detection. Refine Polymer Bond using sm-1) P P odrayt-s yatpyi 2G2 srcmeddfrthe 160. for page to refer concentrate, recommended Novocastra a as is available Also differentiation. and (27G12) origin neuroendocrine of Synaptophysin tumors of neuro- identification of ready-to-use spectrum wide Bond a in Information expressed Specific Product be junctions. to neuromuscular reported in tumors. is endocrine as exocytosis such and well formation as vesicle as and synaptic in similar medulla involved in be adrenal and may retina Synaptophysin the cord, spinal of brain, occur in to vesicles neurons reported of is vesicles It presynaptic glycoprotein. in membrane integral an is Synaptophysin Use Diagnostic Vitro In PA0299 For ready-to-use Bond mL 7 27G12 Clone Synaptophysin cdPopaae(65 sn odPlmrRfn Detection. Refine Polymer Bond using Tartrate-Resistant (26E5) ready-to-use Phosphatase Bond Acid with staining immunohistochemical leukemia: cell Hairy 161. page to refer and concentrate, Novocastra leukemias a as cell available Also T macrophages and tissues. some embryonic cell and and maternal B throughout syncytiotrophoblasts distributed various cells, with and decidual associated spleen lymphomas, also are be the levels Elevated in to turnover. increased reported bone active the be undergoing and individuals disease to of Hodgkin's sera disease, reported 4-nitrophenyl and Gaucher's with is spleen individuals of osteoclasts, and TRAP monocytes macrophages, of alveolar ATP Expression human liver. in phosphoproteins, applications expressed different be through towards reported to been has isoenzyme activity protein This phosphate. iron-binding high basic, a is with (TRAP) phosphatase acid Tartrate-resistant Use Diagnostic Vitro In PA0093 For ready-to-use Bond mL 7 26E5 Clone (TRAP) Phosphatase Acid Tartrate-Resistant cells. tumor of staining cytoplasmic intense Note Detection. Refine using Polymer (27G12) Bond Synaptophysin ready-to-use Bond with staining immunohistochemical Carcinoid: W P F etr blotting Western Paraffin Frozen New! C I lwcytometry Flow Immunofluorescence (HIER) P (HIER) P O E te applications Other lcrnmicroscopy Electron /41
Bond Bond o ealdifraino l rdcspes ii u website: our visit please www.leica-microsystems.com products all on information detailed For /42 Detection. Refine Thyroglobulin Polymer ready-to-use Bond Bond using (1D4) with staining immunohistochemical thyroiditis: Hashimoto's 162. page to refer the concentrate, Novocastra by a as synthesized available Also subunits, thyroid. identical the of two cells epithelial of follicular composed is Thyroglobulin kD 670 of protein glycosylated heavily A Use Diagnostic Vitro In For PA0025 ready-to-use Bond mL 7 1D4 Clone Thyroglobulin Detection. Refine Polymer Bond using Deoxynucleotidyl (SEN28) Terminal Transferase ready-to-use Bond with staining immunohistochemical Thymus: 162. page to refer concentrate, Novocastra a as available Also lymphoma lymphomas. leukemia/ other in lymphoblastic from acute use of for differentiation and recommended diagnosis is the (SEN28) Transferase Deoxynucleotidyl Terminal lymphoblastic between Information histologically Specific Product differentiate to lymphoma. Burkitt's difficult valuable and most lymphoma is be to it is reported is differentiation expression when normal precise TdT of the and and determination leukemias of early The in crucial. lymphocytes expressed where be B lymphomas is to lymphoblastic reported and It is acute T TdT polymerase. marrow. primitive bone DNA in and thymus a expressed be is to (TdT) reported Transferase Deoxynucleotidyl Terminal Use Diagnostic Vitro In PA0339 For ready-to-use Bond mL 7 SEN28 Clone Transferase Deoxynucleotidyl Terminal P (HIER) P iutr ln:imnhsohmclsann ihBn ed-oueTyodStimulating Thyroid Detection. ready-to-use Refine Polymer Bond Bond with using (QB2/6) staining Hormone immunohistochemical gland: Pituitary 163. page to refer concentrate, is Novocastra pituitary a as and TSH available pituitary Also hormones. the of thyroid cells which thyrotrophic of kD in 28 adenomas. production expressed of be and hormone to pituitary reported growth a thyroid is (TSH) stimulates hormone stimulating Thyroid Use Diagnostic Vitro In PA0776 For ready-to-use Bond mL 7 QB2/6 Clone Hormone Stimulating Thyroid ugtsu:imnhsohmclsann ihBn ed-oueTyodTranscription Thyroid ready-to-use Detection. Bond Refine Polymer with Bond using (SPT24) staining Factor immunohistochemical tissue: Lung 163. the page to in refer concentrate, Novocastra use a as for available Also lung. recommended and thyroid is the of (SPT24) tumors of proper classification Factor-1 the Transcription may Thyroid for expression abnormal essential Information since Specific be Product lungs; abnormalities. to and congenital of number TTF-1 thyroid a Gene underline the shown proteins. of surfactant have genes development and regulating encoding studies protein genes in secretory the targeting include cell role These brain. Clara a and thyroglobulin, lung plays thyroid, the (TTF-1) in expressed Factor-1 Transcription Thyroid Use Diagnostic Vitro In PA0364 For ready-to-use Bond mL 7 SPT24 Clone Factor-1 Transcription Thyroid hsctlgi o o s nteUSA. the in use for not approval. is regulatory catalog to This subject are catalog this in Products (Enzyme) P (HIER) P loaalbea ooatacnetae ee opg 170. of page to indicators refer concentrate, significant Novocastra most a as the available Also of one colonic of be tissue- polarity neoplasia. to or shows of reported dysplasia loss Villin is morphological maximum villus. cells The domains, the The epithelial polarity. apical of their their so. and indicating base epithelia gradually do thus certain the villi, to at they restricted the expression, occurs of of as specific tips villin migration phenotype the intestinal of a to differentiated production the crypts involves intestinal their of this the and acquiring cells from types renewed is Epithelial cell villin being types, these cytoplasm. brush continually cell apical a these are the In of lack mucosa the liver. cells which the duct in of epithelia as cells such concentrated some biliary gut in embryonic and from pancreas found derived exocrine be are which to but reported border also is tubules Villin proximal kidney testis. the intestinal the of the of cells efferentes of epithelial ductuli cells in and by or epithelial bladder, produced border. gall mainly brush and is a mucosa Villin develop actin. that all of cells severin, assembly epithelial and and fragmin framework gelsolin, the proteins regulate structurally-related the and Villin Use Diagnostic Vitro In PA0106 For ready-to-use Bond mL 7 CWWB1 Clone Villin Detection. Refine Polymer Bond Tyrosinase using ready-to-use (T311) Bond with staining immunohistochemical melanoma: Malignant using 167. page to assessed refer concentrate, Novocastra be a as can available Also lesions melanocytic (T311). by in Tyrosinase catalyzed expression is melanomas. Tyrosinase and dopaquinone melanocytes in to reported is conversion expression for whose in its substrate tyrosinase, and and initial albinism the biosynthesis of is melanin forms L-tyrosinase various albinism. oculocutaneous with particular associated is deficiency a Tyrosinase enzymes, of tyrosinase. family is a which involves of melanocytes member key in melanin of biosynthesis The Use Diagnostic Vitro In PA0322 For ready-to-use Bond mL 7 T311 Clone Tyrosinase (HIER) P (HIER) P nohla el,sot uce eaoye swl sTadB and T 170. as page to refer well concentrate, fibroblasts, Novocastra a as as including available Also melanocytes origin, muscle, mesenchymal be to smooth of reported lymphocytes. is cells cells, filament intermediate most endothelial an is filamentous in that cytoplasmic protein expressed kD of 57 a types Vimentin, of (IF). filaments number intermediate-sized and microfilaments a microtubule, proteins, contain cells Eukaryotic Use Diagnostic Vitro In PA0033 For ready-to-use Bond mL 7 SRL33 Clone Vimentin Bond using Detection. (CWWB1) Refine Villin Polymer ready-to-use Bond with staining immunohistochemical Colon: loaalbea ooatacnetae ee opg 123. page to refer concentrate, Novocastra a as available Also hemagiomas, including in platelet sarcomas. tumors, expressed Kaposi's be of and to to number hemagiosarcomas reported a is domains and and Willebrand megakaryocytes cells Von binding platelets, endothelial heparin. by functional and synthesized is collagen has factor Ib/IIIa, glycoprotein factor Ib, injured VIII. factor Willebrand kD glycoprotein coagulation to for 270 von stabilizer adhesion and a carrier platelet The a is as mediates serves antigen) and It VIII-related walls vessel factor glycoprotein. (or plasma factor multimeric Willebrand von Human Use Diagnostic Vitro In PA0400 For ready-to-use Bond mL 7 36B11 Clone Factor Willebrand von Detection. Refine Polymer (SRL33) Bond Vimentin using ready-to-use Bond with staining immunohistochemical Rhabdomyosarcoma: W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence (HIER) P (HIER) P O E te applications Other lcrnmicroscopy Electron /43
Bond Bond o ealdifraino l rdcspes ii u website: our visit please www.leica-microsystems.com products all on information detailed For /44 Detection. (WT49) Refine tumor Polymer Wilms' Bond ready-to-use using Bond with staining immunohistochemical tumor: Wilms' 170. page to refer concentrate, Novocastra round a small cancers as type available human sub Also to of antibodies of range panel a tumors. tumor a of cell part Wilms' blue in as mesothelioma. used expressed and often tumor is leukemia be protein Wilms' myeloid to acute mesothelium. breast, reported and including of tract also range 20 another urogenital to is in embryonic 10 the present protein only in be from the in may 1 observed derived but from are affects tumors, tumors gene the Wilms' and arise WT1 sporadic the in array of to of percent disorganized Mutations result reported births. a live activity in is Denys- 10,000 cells this and tumor mesenchyme tumor destroy point The metanephric Wilms' or which syndrome. nephroblastoma deletions childhood gene Drash any the and WT1 of domain the development binding The of DNA oncogenesis. of a in mutations Alteration contains mechanism cells. is common hematopoietic protein a and of WT1 is regulation subset function transcriptional a factor in and transcription kidney role the a in has expressed (WT1) protein tumor Wilms' Use Diagnostic Vitro In PA0562 For ready-to-use Bond mL 7 WT49 Clone Tumor Wilms' Detection. Refine Polymer Factor Bond Willebrand using von (36B11) ready-to-use Bond with staining immunohistochemical Endothelium: (HIER) P loaalbea ooatacnetae ee opg 171. page to refer concentrate, Novocastra a associated as be available Also to reported been phenotype. has aggressive expression non-mutated more sub-type level a high positive arewith the ZAP-70 the with The correlated that detectable CD38. is express Leukemic this express of not adults. protein and do healthy protein generally ZAP-70 of ZAP-70 that genes of cells levels IgVH T of mutated cells blood express the that levels in cells B found express those triggers to that CLL genes comparable activation cells receptor T VH response. cell on T expressed immunoglobulin the is immune for It required proteins. is of an and family cells syk the NK of and member a is ZAP-70 Use Diagnostic Vitro In PA0998 For ready-to-use Bond mL 7 L453R Clone ZAP-70 osl muoitceia tiigwt odrayt-s A-0(43)uigBond using (L453R) Refine ZAP-70 Polymer ready-to-use Bond with staining immunohistochemical Tonsil: hsctlgi o o s nteUSA. the in use for not approval. is regulatory catalog to This subject are catalog this in Products Detection. (HIER) P Bond Ready-to-UseISHProbes M a euti esrnua ern os ontv,mtradvisual and motor cognitive, loss, hearing sensorineural seizures. congenital and in asymptomatic, deficits are result children can of intrauterine of majority CMV result the patients, a although is AIDS and CMV as Congenital infection neonates. causing such viral in of and individuals subsequent patients capable immunocompromised transplant pathogen, and in opportunistic infection disease common latent serious a fluids, body infection, is via CMV primary transmitted reactivation. family, establish Virus Herpes can Beta the and of member a is Detection CMV Refine Polymer Bond Application with use (AR0833/AR0222). Antibody Anti-Fluorescein paraffin- for and formalin-fixed, (DS9800) Optimized in transcript tissue. 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Bond Bond o ealdifraino l rdcspes ii u website: our visit please www.leica-microsystems.com products all on information detailed For /50 hsctlgi o o s nteUSA. the in use for not approval. is regulatory catalog to This subject are catalog this in Products Novocastra. to switch effi and to time consistency it’s ciency, quality, For reagents. ancillary and systems, antibodies, Novolink antibodies, Novocastra with slides IHC superior Create ANTIBODIESPRIMARY NOVOCASTRA TM Compact Polymer Compact TM detection detection TM rmr Antibodies Primary W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence O E te applications Other lcrnmicroscopy Electron /51
Primary Antibodies A BETTER WAY TO SELECT SUPERIOR REAGENTS Pathologists and scientists can quickly identify the antibodies they need with the Novocastra Reference Range. Each antibody clone in the range was selected for its superior performance then grouped according to its applications. • Quality – antibodies tested in formalin-fixed, paraffin-embedded tissue • Consistency – a complete solution including detection systems, diluents and ancillaries • Reliability – robust mouse monoclonal antibodies
NOVOCASTRA™ REFERENCE RANGE™ Primary Antibodies
You can identify Reference Range products in this
catalog by looking for this symbol: Reference Range
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. a-0L5RNCL-L-ZAP-70 NCL-L-VIM-572 NCL-L-WT1-562 L453R NCL-L-S100p SRL33 WT49 NCL-L-PAX-5 NCL-L-TTF-1 Polyclonal NCL-p63 NCL-L-PGR-312 NCL-PSA-431 NCL-OCT2 NCL-RCC NCL-L-NapsinA NCL-L-TdT-339 1EW NCL-L-p53-DO7 SPT24 7JUL Oct-207 66.4.C2 35H9 Zap-70 16 SEN28 IP64 Wilms’ Tumor NCL-L-MelanA DO-7 Vimentin Thyroid Transcription Factor-1 NCL-L-MSH6 Terminal DeoxynucleotidylTransferase NCL-L-Ki67-MM1 NCL-MSH2 S-100 NCL-L-LAM-578 NCL-L-MLH1 Renal CellCarcinomaMarker NCL-HMB45 A103 NCL-L-KAP-581 Prostate Specific Antigen NCL-L-IgM Progesterone Receptor PU29 Pax-5 MM1 NCL-L-IgD 25D12 p63 Protein SHL53 NCL-L-IgA HMB45 ES05 p53 Protein(DO-7) CH15 Oct-2 NCL-HPp NCL-L-ER-6F11 8H6 Napsin NCL-L-DOG-1 NCL-L-FRalpha DRN1C Mismatch RepairProtein(MSH6) N1CLA Mismatch RepairProtein(MSH2) Polyclonal Mismatch RepairProtein(MLH1) NCL-ESA NCL-L-CK20-561 Melanoma Marker(HMB45) NCL-MOC-31 BN3.2 6F11 NCL-L-LL002 Melan A NCL-L-CK7-560 K9 Lambda LightChain NCL-L-CK5 Ki67 Antigen NCL-L-EGFR-384 Kappa LightChain VU-1D9 NCL-L-CYCLIND1-GM PW31 MOC-31 NCL-CDX2 Immunoglobulin M LL002 Immunoglobulin D RN7 NCL-L-CD79a-225 Immunoglobulin A XM26 NCL-L-CD68 Helicobacter pylori EGFR.25 NCL-L-AE1/AE3 P2D11F11 Folate ReceptorAlpha AMT28 NCL-L-CB11 Estrogen Receptor NCL-L-CD56-1B6 NCL-L-CD117 Epithelial Specific Antigen 11E3 NCL-L-CD33 Epithelial-Related Antigen AE1/AE3 514H12 Epidermal GrowthFactorReceptor NCL-L-CD30-591 DOG-1 NCL-L-CD23-1B12 CB11 Cytokeratin 20 NCL-L-CD20-7D1 1B6 T595 Cytokeratin 14 PWS44 NCL-L-CD19-163 Cytokeratin 7 JCM182 NCL-L-CD15 Cytokeratin 5 NCL-L-END NCL-L-CD11c-563 Cytokeratin Multi(AE1/AE3) NCL-L-CD10-270 1B12 NCL-CD25-305 NCL-L-CD8-295 Cyclin D1 7D1 c-erbB-2 Oncoprotein(HER2) NCL-L-CD7-580 BT51E NCL-L-CD5-4C7 QBEnd/10 CDX2 BY87 NCL-LCA CD117 (c-kitOncoprotein) NCL-L-CD4-368 5D11 CD79a NCL-L-CD3-565 56C6 4C9 CD68 1A5 NCL-L-CD1a-235 CD56 (NCAM) LP15 CD45 X16/99 4C7 NCL-L-CALRET-566 CD34 (EndothelialCellMarker) 4B12 CD33 LN10 CD30 MTB1 NCL-L-Bcl-6-564 CD25 (Interleukin-2Receptor) NCL-bcl-2-486 CD23 CAL6 CD20 ProductCode CD19 NCL-L-Akt-Phos CD15 LN22 CD11c 3.1 CD10 Clone CD8 CD7 NCL-ALK LP18 CD5 CD4 NCL-L-BOB-1 CD3 CD1a Calretinin 5A4 Bcl-6 Oncoprotein bcl-2 Oncoprotein TG14 B CellSpecific OctamerBindingProtein-1(BOB-1) Akt (Phosphorylated) Anaplastic Lymphoma Kinase (ALK) Name W P F Breast IHC etr blotting Western Paraffin Frozen Leukemia IHC Lymphoma IHC C I Melanoma IHC lwcytometry Flow Immunofluorescence GI Tract IHC Prostate IHC O E Lung IHC te applications Other lcrnmicroscopy Electron Renal IHC
Special IHC /53
Primary Antibodies Adenomatous Polyposis Coli Protein (APC)
Clone EMM43 1 mL lyophilized NCL-APC P See also APC (Adenomatous Polyposis Coli Protein) on page 58.
Human skin: immunohistochemical staining for phosphorylated Akt using NCL-L-Akt-Phos. Adenovirus Note intense nuclear staining. Paraffin section.
Clone 10/5.1.2 1 mL lyophilized NCL-ADENO I The Adenoviridae are a family of double-stranded DNA viruses. They may ALCAM (CD166) cause a variety of infections involving respiratory, ocular, genito-urinary or enteric systems. Adenoviruses may cause life-threatening infections in Clone MOG/07 transplant recipients, AIDS patients and immunocompromised patients. 1 mL, 0.1 mL lyophilized NCL-CD166 P (HIER) Product Specific Information See also CD166 (ALCAM) on page 88. NCL-ADENO is a pan adenovirus specific reagent. Reactivity has been confirmed with adenovirus serotypes 1 to 7, 40 and 41 as primary isolates in tissue culture. NCL-ADENO does not cross-react with tissue culture isolates of respiratory syncytial virus, influenza virus types A and B, parainfluenza virus types 1, 2, 3 and 4b, herpes simplex virus types 1 and 2, varicella-zoster ALK (Anaplastic Lymphoma Kinase) virus, cytomegalovirus, mumps virus, measles virus, echovirus 19, coxsac67e B4 virus, poliovirus types 1, 2 and 3 or negative tissue culture cells used in (CD246) (p80) routine virus isolation. Primary Antibodies Clone 5A4 1 mL, 0.1 mL lyophilized NCL-ALK P (HIER) Reference Range 7 mL Bond ready-to-use PA0306 P (HIER) Anaplastic large cell lymphoma (ALCL) is usually composed of large pleomorphic cells which are reported to express CD30 antigen and the epithelial membrane antigen (EMA). These tumor cells tend to occur in younger individuals and may be associated with cutaneous and extranodal involvement. A proportion of these cases contain a chromosomal translocation t(2;5) (p23; q35). This results in a hybrid gene encoding part of the nucleophosmin (NPM) gene joined to the cytoplasmic domain of the anaplastic lymphoma kinase (ALK) gene, giving rise to the protein, p80. Human nasopharyngeal secretion: immunofluorescence for Adenovirus using NCL-ADENO. Large cell lymphomas account for approximately 25 percent of all non- Note intense staining of Adenovirus infected respiratory epithelial cells. Acetone-fixed cells. Hodgkin's lymphomas in children and young adults, of which one third carries the NPM-ALK gene translocation. Refer to page 14 for the Bond ready-to-use format. Akt (Phosphorylated)
Clone LP18 1 mL, 0.1 mL liquid NCL-L-Akt-Phos P (HIER) W Reference Range Akt-1, also referred to as Protein Kinase B (PKB) or Rac alpha is a member of the Akt serin /threonine protein kinase family. It plays an important role in many biological responses including metabolism, cell survival and growth by phosphorylation and inactivating several targets including GSK 3 beta, caspase 9, BAD and the Forkhead transcription factor. Product Specific Information Human anaplastic lymphoma: immunohistochemical staining for anaplastic lymphoma NCL-L-Akt-Phos is not recommended for use with PBS, since the use of PBS- kinase (p80) using NCL-ALK. Note cytoplasmic staining of large pleomorphic cells. Paraffin based wash buffers and possibly PBS-based antibody diluents gives section. increased background staining and decreased staining intensity. Proprietory reagents from Leica or TBS-based wash buffer and diluents are recommended.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. C-1p oectpamcsann ftmrcls aafnsection. Paraffin cells. tumor of using staining alpha-1-antitrypsin cytoplasmic for Note staining NCL-A1Ap. immunohistochemical tumor: sac yolk egg Human or childhood later to in lead present may may deficiency individual alpha-1-antitrypsin an cirrhosis. with liver, or adulthood the hepatitis In neonatal disease. and lung. emphysema the liver panacinar in with eg associated by is destruction deficiency tissue released Alpha-1-antitrypsin elastase-induced elastase uninhibited of in deficiency result neutralization Alpha-1-antitrypsin may the response. inflammatory be elastase. an and to particularly during serum neutrophils proteases, appears of in function inhibitor present an main is as Its acts and it liver where fluids the tissue in synthesized is Alpha-1-antitrypsin NCL-A1Ap lyophilized mL 1 Polyclonal Alpha-1-Antitrypsin section. Paraffin cells. malignant of staining cytoplasmic intense Note NCL-A1ACp. alpha-1-antichymotrypsin using for staining immunohistochemical melanoma: malignant Human major via a that ACT. discovered to shown complexed also exists serum been has in cells (PSA) research astroglial antigen of Further prostate-specific also and cytoplasm of abnormalities. the proportion breast and brain have cells it certain that endothelial cells, shown fibrils, in has synthesis amyloid Research endothelial brain. in the found cells, in of is exists agent also mast ACT anti-inflammatory cells. levels epithelial in intestinal an Lower analysis as act immunohistochemical of hepatocytes liver. to by primarily synthesized thought the is chemotaxis ACT neutrophil is production. chymase, anion it cell superoxide and mast but G, cathepsin a uncertain chymotrypsin, role precise and inhibiting is The protein superfamily. ACT serpin phase or inhibitor of acute proteinase serine early-stage the of an member is (ACT) Alpha-1-Antichymotrypsin NCL-A1ACp lyophilized mL 1 Polyclonal Alpha-1-Antichymotrypsin Ezm)W (Enzyme) P F P nacsoypoenfritreit iaetageain lh B Alpha aggregation. renal bodies including carcinomas filament various as in intermediate carcinoma. expressed function Lewy cell be may to for it reported but is protein unknown, as crystallin is bodies bodies accessory inclusion role Mallory such The in an tangles. crystallin ubiquitinated and neurofibrillary B in in Alpha filaments) found bodies, of rarely found is (glial it be However, fibers inclusion (cytokeratins). to Rosenthal reported filament (neurofilaments), is squamous stratified crystallin muscle, and intermediate B skeletal epithelium cells, Alpha as colonic Schwann epithelium, such epithelium, epithelium. thyroid tubular tissues small renal cells, in the muscle, with glial expressed homology smooth is some muscle, has It cardiac that proteins. protein shock lens a heat is crystallin B Alpha NCL-ABCrys-512 lyophilized mL 0.1 mL, 1 G2JF Clone Crystallin B Alpha discs discs. Z Z the the of of polymers that lateral to be as to similar thought structure are known lattice rods a the particles, and have shown have rods threadlike studies nemaline microscopy the abundant Electron that myofibers. of the in Muscle is presence bodies, region. nemaline alpha-actinin this the in muscle, F-actin show with skeletal cross-links tissues and In band/disc Z utrophin. the family in and same located the dystrophin to belonging spectrin, protein as cytoskeletal rod-like a is Alpha-actinin NCL-alpha-ACT lyophilized mL 0.1 mL, 1 RBC2/1B6 Clone Alpha-Actinin C-Brs52 oeitnectpamcsann fmlgatcls aafnsection. Paraffin cells. malignant of using staining crystallin cytoplasmic B intense alpha Note for NCL-ABCrys-512. staining immunohistochemical carcinoma: cell renal Human W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence FW HE)W (HIER) P F O E te applications Other lcrnmicroscopy Electron /55
Primary Antibodies Alpha-Catenin Alpha-Internexin
Clone 25B1 Clone 2E3 1 mL lyophilized NCL-A-CAT F P (HIER) W 1 mL lyophilized NCL-A-INTER P (HIER) Alpha-catenin, which shows some homology with vinculin, appears to play a Alpha-internexin is a 66 kD protein which shares several characteristics role in tumor invasion and metastasis through the dysfunction of E-cadherin. with intermediate filament proteins. In rats, alpha-internexin is often co- Normal epithelium of the esophagus, stomach and colon express alpha- purified with intermediate filaments from spinal cord and optic nerve. The catenin strongly, without exception. However, in primary tumors of these protein is axonally transported together with the neurofilament triplet tissues its expression is frequently reduced. It has been suggested that proteins (68, 160 and 200 kD) along the length of the optic nerve. Although some human cancer cells may have impaired E-cadherin-mediated cell the distribution of alpha-internexin is similar to the light component adhesiveness as a result of the downregulation of alpha-catenin expression. neurofilament (68 kD), its distribution in rat embryo is far more extensive in Abnormalities in the expression of alpha-catenin seem to associate with the early stages of development. There is also evidence that expression of malignant cellular features and disease progression in prostate cancer. In alpha-internexin is heavily upregulated in damaged neurons. one study of several tumor-related proteins in breast cancers, it was shown that alpha and beta-catenins could be re-expressed in metastatic tissue. Re- expression of these adhesion molecules by tumor cells after release from the primary site may be important and perhaps necessary for cells to adhere in remote organs.
Human brain, basal ganglia: immunohistochemical staining for alpha-internexin using NCL-A-INTER. Note intense cytoplasmic staining of white matter tracts. Paraffin section. Primary Antibodies Human appendix: immunohistochemical staining for alpha catenin using NCL-A-CAT. Note intense membrane staining of the mucosal epithelial cells. Paraffin section. Alpha-Methylacyl-CoA Racemase (AMACR, p504s) Alpha Fetoprotein Clone EPUM1 New! 1 mL, 0.1 mL liquid NCL-L-AMACR P (HIER) Clone C3 Alpha-methylacyl-CoA racemase (AMACR), also known as p504s, is a 1 mL, 0.1mL lyophilized NCL-AFP FP mitochondrial and peroxisomal enzyme that is involved in bile acid biosynthesis and beta-oxidation of branched-chain fatty acids. AMACR is 7 mL Bond ready-to-use PA0963 P essential in lipid metabolism, and is expressed in normal liver (hepatocytes), Alpha fetoprotein (AFP) is an oncofetal antigen of 70 kD found in body fluids kidney (tubular epithelial cells) and gall bladder (epithelial cells). Expression which if detected in high concentrations has clinical implications. AFP is has also been found in lung (bronchial epithelial cells) and colon (colonic expressed in fetal liver but is not present under normal circumstances in surface epithelium). Expression is granular and cytoplasmic. AMACR healthy adult tissues. It is reported to be expressed in a proportion of germ expression can also be found in hepatocellular carcinoma and kidney cell tumors, with high frequency in yolk sac tumors. carcinoma. Past studies have also shown that AMACR is expressed in various colon carcinomas (well, moderately and poorly differentiated) and Refer to page 14 for the Bond ready-to-use format. over expressed in prostate carcinoma.
Human fetal liver: immunohistochemical staining for alpha fetoprotein using NCL-AFP. Human prostatic adenocarcinoma: immunohistochemical staining for alpha-methylacyl-CoA Note cytoplasmic staining of hepatocytes. Paraffin section. racemase (AMACR, p504S) using NCL-L-AMACR. Paraffin section.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. LBn ed-ouePA0943 ready-to-use Bond mL 7 RTU-SMA ready-to-use mL 7 NCL-SMA lyophilized mL 1 C--SN oesann fapasncencnann eybde.Prfi section. Paraffin bodies. Lewy synuclein-containing using alpha synuclein of alpha staining for Note staining NCL-L-ASYN. immunohistochemical dementia: body Lewy brain, Human beta- with unreactive acid formic and percent 100 alpha-synuclein recommended. to also 98 is with for sections tissue specific of sporadic Pretreatment synuclein. is in KM51 neurons Clone of Information degeneration Specific Product selective dementia. body of the Lewy formation and disease the and Parkinson's in brain bodies implicated dementia be body Lewy may aggregates Lewy Alpha-synuclein insoluble blot and alpha-synuclein. from truncated Western of partially bodies vesicles. full-length, Lewy synaptic shown has of of purified material membrane surface highly inner outer the of the to of analyses to protein function or target The a terminals synaptosomes. carry nerve to for with be affinity alpha-synuclein may of its alpha-synuclein interaction with direct consistent a suggests membranes homology including This apolipoproteins, E. exchangeable those Apo of resembling domain residue lipid-binding structure the secondary eleven in alpha-helical the found All an around protein. and beta- based intracellular motif the organization an with repeating is unusual of not it an homology does member that have It suggesting sequence synucleins a species. sequence vertebrate signal percent and between a conserved acids 61 possess highly amino is shares and 140 It synuclein of family. protein synuclein a is Alpha-synuclein NCL-L-ASYN liquid mL 1 NCL-ASYN lyophilized mL 1 KM51 Clone Alpha-Synuclein format. ready-to-use Bond the for 41 page to clinical Refer cases. some the in staining with enhance may pretreatment together Enzyme antibodies of Information Specific panel Product they pathologist. situation a a by diagnostic interpreted within and differential data a presentation used in be myo- used and are must the antibodies myofibroblasts for adenomas. such tool intestinal in useful pleomorphic When a and expressed be leiomyosarcomas walls, to leiomyomas, be reported various of are vascular to identification SMA of to reported antibodies stroma in and the also cells epithelial in found is cytoskeletal and It is of propria tissues. muscularis system actin and microfilament mucosae muscle the muscularis of Smooth part proteins. are actins Cytoplasmic Clone (SMA) Actin Muscle Smooth Alpha sm-1 (HIER) P Ezm)W (Enzyme) P F (HIER) P (Enzyme) P F P om fayod mli rti sacntteto omlbasement normal of constituent a 10 network. is fiber comprises elastic protein microfibrillary component, system the P nervous and P central Amyloid membranes the amyloid. but as all of in known present forms is also and P tissue amyloid amyloid protein, of called percent P glycoprotein shaped Amyloid pentagonal protein. with a together of fibrils, protein aggregates non-branching rod-like rigid, of mainly consists Amyloid NCL-AMP lyophilized mL 1 B5 Clone Protein P Amyloid eosin and hematoxylin to in often observe immunohistochemistry, is to difficult by amyloidosis) be biopsies may (secondary preparations. it rectal amyloidosis as tissue or human worthwhile in AA-type protein Such renal A death. characterise amyloid eg of It eventual texture. detection waxy the and a biopsies, vital that develop function and reported of colour been of in has cells pale loss firm, parenchymatous enlarged, gradual become organs the in amorphous replacing resulting of organs, progressively deposition confluent forming and the tissues body by masses various in characterised material extracellular disease eosinophilic a is Amyloidosis NCL-AAC lyophilized mL 1 mc1 Clone Component A Amyloid p80 napesr okrmyb eurdfru o5mntsi re for order in minutes 5 to up for optimally. required work be to may NCL-APP-228 cooker EDTA 1mM pressure in a Unmasking in brain. (pH8.0) disease Alzheimer's of tangle- matter neuropil gray and neurofibrillary in plaques threads senile late-stage surrounding processes with neuritic neurons, astrocytes reacts bearing neurons, NCL-APP-228 smaller as proteins. microglia. well as APP-like and NCL- cells proteins. pyramidal the amyloid large APP-like with with with beta reacts cross-react homology APP the not least do and NCL-APP the and domain shows NCL-APP-228 inhibitor region protease of This portion Kunitz region. extracellular the the to between raised APP been have NCL-APP and NCL-APP-228 Information Specific Product and with glycoproteins structure. Presenilin-1 domain transmembrane Alzheimer's modular are similar of (APP), proteins a forms APP-like familial and protein cause APP identified can disease. precursor have mutated, studies when elderly, which, beta-amyloid Genetic the Presenilin-2 forms. in genes; sporadic dementia and of three familial cause both common in most exists the disease, Alzheimer's NCL-APP lyophilized mL 1 40.10 Clone NCL-APP-228 lyophilized mL 1 3G12 Clone Protein Precursor Amyloid ethanol. percent 70 in fixed been paraffin- normal has for in tissue suitable the protein only when is P material NCL-AMP amyloid embedded deposits. of amyloid in identification and the tissue for human used be may NCL-AMP Information Specific Product W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence (HIER) P P FP (HIER) P O E te applications Other lcrnmicroscopy Electron /57
Primary Antibodies AP-2 Gamma
Clone GIA50 1 mL lyophilized NCL-AP2G P (HIER) W The AP-2 transcription factors are required for normal growth and morphogenesis during mammalian development. Initial in vitro studies have also indicated that the AP-2 family of proteins are involved in the etiology of human breast cancer. The various AP-2 genes are expressed in many human breast cancer cell lines and critical AP-2 binding sites are present in both c-erbB-2 and estrogen receptor promoters. AP-2 gamma has been Human cortex, Alzheimer's disease: immunohistochemical staining of amyloid precursor shown to be expressed in normal breast myoepithelial cells and to be protein using NCL-APP-228. Note intense staining of neurofibrillary tangles and senile plaques. upregulated in a proportion of breast cancer specimens. AP-2 gamma Paraffin section. expression has been shown to be upregulated in the trophoblast lineage throughout development, suggesting a crucial role for both trophoblast development and differentiation. Gene expression and antibody studies have indicated that AP-2 gamma expression occurs in testis within oogonia/ gonadocytes and was downregulated with germ cell differentiation. Several Anaplastic Lymphoma Kinase (ALK) studies have since indicated that AP-2 gamma may be useful in the (CD246) (p80) identification of testicular derived tumors. Product Specific Information Clone 5A4 NCL-AP2G has been shown, through immunohistochemistry, ELISA studies and Western blotting to be specific for the AP-2 gamma transcription factor. 1 mL, 0.1 mL lyophilized NCL-ALK P (HIER) Reference Range
7 mL Bond ready-to-use PA0306 P (HIER) AB See also ALK (Anaplastic Lymphoma Kinase) (CD246) (p80) on page 54. 97.4 kD - 66.2 kD - - AP-2 gamma 45.0 kD - Primary Antibodies Androgen Receptor 31.0 kD -
Clone AR27 21.0 kD - 1 mL, 0.1 mL lyophilized NCL-AR-318 F P (HIER)
Western blot: detection of human AP2 gamma protein using NCL-AP2G. Lane A, molecular Clone 2F12 weight markers. Lane B, SKBR3 cell line immunoblotted with NCL-AP2G. 1 mL lyophilized NCL-AR-2F12 F P (HIER) Clone AR27 was developed to produce superior staining to clone 2F12 on paraffin sections. Androgen Receptor is a member of the superfamily of ligand responsive APC (Adenomatous Polyposis Coli transcription regulators. The androgen receptor functions in the nucleus where it is believed to act as a transcriptional regulator mediating the action Protein) of male sex hormones (androgens). The androgen receptor has wide distribution and can be demonstrated by immunohistochemistry in several Clone EMM43 tissues eg prostate, skin, and oral mucosa. Androgen receptor has been reported in a diverse range of human tumors eg osteosarcoma and in 1 mL lyophilized NCL-APC P prostatic carcinoma androgen receptor expression may be of clinical The human adenomatous polyposis coli (APC) gene at locus 5q21 encodes a relevance. Furthermore, mutation of the gene encoding androgen receptor protein of 2,843 amino acids. A precise role for APC in the regulation of the has been reported in prostatic carcinoma. wnt/beta-cateninin signalling pathway has been clearly recognized. APC forms molecular complexes which are able to eliminate intra-cytoplasmic beta-catening, inducing its degradation. It is expressed in the cytoplasm of epithelial and mesenchymal cell types. In the epithelium of bladder, small and large intestine, esophagus, stomach and epidermis, APC expression is restricted to regions in which cell replication has ceased and terminal differentiation has been established. Expression has been reported in lung, kidney and mammary gland endothelial, myoepithelial and duct lining epithelial cells. Some tissues such as ovary, myometrium, thyroid, parathyroid and tonsil do not express the protein. Mutations of the APC gene have been linked to the development of sporadic colorectal tumors, as well as familial adenomatous polyposis and cancers of the pancreas, stomach and esophagus. APC mutations have also been observed at significantly Human prostatic adenocarcinoma: immunohistochemical staining for androgen receptor using high frequency in the advanced stages of breast cancer suggesting a NCL-AR-318. Note nuclear staining of tumor cells. Paraffin section. biological role in carcinogenesis.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. i n ontbcm motlzd niewt h ciekinase. active eventually the with cells unlike the immortalized, become however, not expressed, aurora do other and is Aurora inhibit die kinase and that mutant inactive suggests negative When This dominant a kinases. polyploidy. as to behave and can lead active 2 can spindle Kinase both 2 of monopolar Kinase Overexpression Aurora a cells. inactive mammalian of in instability chromosome transformation formation Kinase amplification, and Aurora centrosome the separation. to The centrosome leads to for overexpression stability. 2 necessary and leads is various activity activity activity its and its because its testis for by in of both regulated levels important is inhibition 2 high is Kinase at Aurora which cells. Aurora expressed phosphorylation HeLa cells. be including AIK, mammalian lines, to cell in and reported proliferating release ARK1 and It is phase poles. BTAK, 2 G2/M spindle mitosis Kinase the after STK15, to during rapidly as and degraded centrosomes activities known is mitotic and also spindle interphase 2, to microtubule localizes Kinase in Aurora involved meiosis. kinases serine/threonine are cycle-regulated cell encode that 2 and 1 Kinase Aurora NCL-L-AK2 liquid mL 1 JLM28 Clone 2 Kinase Aurora activated Once apoptosis. caspase-9. and a activation in ATP, caspase resulting of or additional directly, of procaspase-3 dATP cascade activate activation of and cleave presence then autocatalytic can the caspase-9 in with the (Apaf-3) associates caspase-9 complex promoting of c form Apaf1/cytochrome zymogen until cell resulting the all state not from The probably latent released but stimuli. many, a commonly death by is in induced c apoptosis remains during Cytochrome mitochondria that (Apaf-2). the factor-1 c protein activating cytochrome cytosolic protease to a apoptosis bound for as is mechanism known of Apaf-1 and protein result identified (Apaf-1). mitochondria kD a of One 130 as participation a cell. occur the involves may requires the activation that in caspase responses initiating of pathways number transduction a signal of one is Apoptosis NCL-APAF1 lyophilized mL 1 Polyclonal 1 Factor Activating Protease Apoptosis 91. page on J) (Apolipoprotein Clusterin also See NCL-CLUSTERIN lyophilized mL 1 7D1 Clone (Clusterin) J Apolipoprotein section. Paraffin cells. epithelial intestinal of staining protein cytoplasmic coli Note polyposis NCL-APC. adenomatous using for staining immunohistochemical intestine: small Human HE)W (HIER) P (HIER) P F (HIER) P F toa el fteoay B a enrpre oratwt an with react to reported been cell B has and cells MB2 B normal lymphomas. both ovary. in found the antigen cytoplasmic uncharacterized of and hepatocytes lung, also cells breast, include and of tube These stromal epithelia fallopian cells. bladder, layer), colon, epithelial cell mature stomach, reactive squamous except of pancreas, weakly the cells types is excludes B antibody several (but all The and epidermis cells. in cells T expressed with endothelial is react that with not marker does It cell cells. B plasma pan a is MB2 NCL-MB2 lyophilized mL 1 MB2 Clone (MB2) Marker Cell B section. Paraffin cells. of nuclear proportion Note a NCL-L-AK2. of using Kinase staining Aurora for staining immunohistochemical line: cell HeLa el.Teepeso fBB1i eltmr a enrpre obe format. to ready-to-use reported Bond the been for 15 has page tumors to plasma Refer cell and B cells in B BOB-1 center variable. of The germinal tissues. expression in lymphoid The be reactive found cells. to in being found reported expression populations been cell It the strongest has B to BOB-1 protein. all contributes motifs. in and coactivator detectable octamer factors of transcriptional transcription activity OCT2 specific transcriptional and OCT1 lymphocyte and with OBF-1 a interacts as known also is (BOB-1), protein-1 OCA-B, binding octamer specific cell B PA0558 ready-to-use Bond mL 7 NCL-L-BOB-1 liquid mL 0.1 mL, 1 TG14 Clone (BOB-1) Protein-1 Binding Octamer Specific Cell B section. Paraffin lymphocytes. intense B Note normal NCL-MB2. of using staining lymphocytes cytoplasmic B for staining immunohistochemical tonsil: Human W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence FP (HIER) P (HIER) P O E te applications Other lcrnmicroscopy Electron /59
Primary Antibodies Bcl-3 Oncoprotein
Clone 1E8 1 mL lyophilized NCL-Bcl-3 F P (HIER) Bcl-3 was first identified as a putative proto-oncogene and was originally isolated through its involvement in the translocation event t(14;19) where it is highly expressed in a subset of chronic lymphocytic leukemias and other B cell neoplasms. The Bcl-3 gene product is also thought to play a role in the immune system through its interactions with the NF-kappaB family of transcription factors to enhance proliferation and to act as a transcription Human tonsil: immunohistochemical staining for B cell specific octamer binding protein cofactor. More specifically, Bcl-3 oncoprotein appears to regulate the (BOB-1) using NCL-L-BOB-1. Note cytoplasmic and nuclear staining of germinal center B cells. activity of homodimeric NF-kappaB p50 subunit and a closely-related Paraffin section. homolog, p52, in a phosphorylation-dependent manner. Although to date, no immunohistochemistry data has been published, Bcl-3 mRNA is found in a number of tissues, including spleen and other lymphoid tissues. bcl-2 Oncoprotein
Clone 3.1 1 mL, 0.1 mL lyophilized NCL-bcl-2-486 P (HIER) W Reference Range
Clone bcl-2/100/D5 1 mL, 0.1mL lyophilized NCL-bcl-2 F P (HIER) W 1 mL liquid NCL-L-bcl-2 F P (HIER) W 7 mL ready-to-use RTU-bcl-2 Human tonsil: immunohistochemical staining for Bcl-3 oncoprotein using NCL-Bcl-3. Note F P (HIER) nuclear staining of a proportion of follicular cells, parafollicular cells and mucosa. Paraffin
Primary Antibodies 7 mL Bond ready-to-use PA0117 P (HIER) section. Clone 3.1 was developed to produce superior staining on paraffin sections. Bcl-2 is a member of a family of proteins that are involved in apoptosis. Bcl-2 is an integral inner mitochondrial membrane protein of 25 kD and has a wide tissue distribution. It is considered to act as an inhibitor of apoptosis. For this Bcl-6 Oncoprotein reason, bcl-2 expression is inhibited in germinal centers where apoptosis forms part of the B cell production pathway. In 90 percent of follicular Clone LN22 lymphomas a translocation occurs which juxtaposes the bcl-2 gene at Reference Range 18q21, to an immunoglobulin gene. This t(14;18) translocation can deregulate 1 mL, 0.1 mL liquid NCL-L-Bcl-6-564 P (HIER) gene expression and bcl-2 over-expression can be demonstrated immuno- 7 mL Bond ready-to-use PA0204 P (HIER) histochemically in the vast majority of follicular lymphomas. Clone P1F6 1 mL, 0.1 mL lyophilized NCL-Bcl-6 F P (HIER) Clone LN22 was developed to produce superior staining compared to clone P1F6 on paraffin sections. Bcl-6 is a proto-oncogene that encodes a Kruppel-type zinc-finger protein of 95 kD and shares homology with other transcription factors. Bcl-6 protein is mainly expressed in normal germinal center B cells and related lymphomas. It has been shown that the Bcl-6 proto-oncogene is involved in chromosome rearrangements at 3q27 in non-Hodgkin's lymphomas and Bcl-6 rearrange- ments have also been detected in 33 to 45 percent of diffuse large B cell lymphomas. Immunohistochemistry has been reported to show the Bcl-6 Human breast carcinoma: immunohistochemical staining for bcl-2 oncoprotein using gene product to be detectable in follicular lymphomas, diffuse large B cell NCL-bcl-2-486. Note membrane staining of tumor cells and also cytoplasmic staining lymphomas, Burkitt's lymphomas and in nodular, lymphocyte predominant in a small proportion of these. Paraffin section. Hodgkin's disease. Refer to page 15 for the Bond ready-to-use format.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. egtmres aeB 59cl ieimnbotdwt NCL-Bcl-10. with immunoblotted molecular line A, cell Lane A549 NCL-Bcl-10. B, using Lane kD) markers. (32 weight oncoprotein Bcl-10 of detection blot: Western Bcl-10 the of region coding the of majority the to oncoprotein. raised is NCL-Bcl-10 with tissues Information Specific lymphoid Product and cytoplasm. breast the in in tissues, that found only normal predominantly factors staining expressed In important is development. oncoprotein be tumor These a Bcl-10 Bcl-10 during to molecule. gain proliferation thought length the cell and full therefore, encourage the apoptosis are, in to induce present molecules not not truncated lead is do that molecules proteins of events function truncated downstream transforming several immediately The mutation region of CARD. the the Several regions in truncated with apoptosis. becoming recruitment oncoprotein homologous caspase in is a involved which encodes The cancer. oncoprotein (CARD) of Bcl-10 forms domain oncoprotein common of many Bcl-10 with region of associated N-terminal be forms to Mutated found circumstances been activation. normal have of NF-kappaB under induces apoptosis (p22;q32) Wild promotes and lymphomas. t(1;14) oncoprotein non-Hodgkin’s extranodal Bcl-10 event of type subset translocation common lymphomas most MALT the the lymphomas. are in (MALT) tissue involvement lymphoid result mucosa-associated direct a as identified its molecule regulatory of apoptotic an is oncoprotein Bcl-10 NCL-Bcl-10 lyophilized mL 1 DAA22 Clone Oncoprotein Bcl-10 section. Paraffin lymphocytes. malignant of staining using nuclear Bcl-6 intense for Note staining NCL-L-Bcl-6-564. immunohistochemical lymphoma; follicular node, lymph Human W pteilclso h rsae atonetnleihla el and cells epithelial in not (but gastrointestinal epidermis the prostate, of layers upper basal the the and in secretory of cells, keratinocytes in epithelial differentiated thymocytes, spermatids mammary cells and in cortical found spermatocytes also epithelial as is in well It as testes. marrow reported the bone differentiating of in been types cells some normal myeloid and In has Reed of precursors cell cases. in blood disease number expression red reported Hodgkin's megakaryocytes, small of been bcl-x percent also a 86 tissues, has about and in It cells centers. areas Sternberg germinal interfollicular within cells, in lymphocytes has plasma Bcl-x in lymphocytes mitochondria. detected with immunohistochemically activated be associated bcl-2, to cytosol like demonstrated the and been to types localized cell many been in has demonstrated been has immunoreactivity C-c-.Nt nes yolsi tiigo ukneclsadterpoess Paraffin processes. their and cells Purkinje of using staining protein cytoplasmic Bcl-w intense for Note staining NCL-Bcl-w. immunohistochemical cerebellum: adult normal brain, Human apparently is is Bcl-w but lines. organs cell most T of and function B and erythroid spermatogenesis. on development for and essential expressed normal megakaryocytic in not macrophage dispensable is lymphoid of few It including a those and muscle, tissues origin. myeloid including of most skeletal in lines range expressed stomach, also diverse cell is heart, a It placenta. in liver, and found testis colon, be hippocampus, to Bcl- man. reported cerebellum, and survival, mice promote is between and cell protein conserved antagonists promotes highly w are is and bax gene and Bcl-w proteins bak The of apoptosis. as such family members Bcl-2 other the whereas to belongs Bcl-w NCL-Bcl-w lyophilized mL 1 6C1 Clone Bcl-w aa cells). basal bcl-2. variants of long and independently short death produces proteins. cell mRNA of bcl-x of bcl-x family as regulator Bcl-2 the known the of a of splicing as member Differential function a is can and Bcl-x with homology has Bcl-x NCL-bcl-x lyophilized mL 1 NC1 Clone bcl-x section. W P F etr blotting Western Paraffin Frozen s n bcl-x and L hs ainshv ifrn ucin.Bcl-x functions. different have variants These . C I lwcytometry Flow Immunofluorescence FP HE)W (HIER) P O E te applications Other lcrnmicroscopy Electron /61
Primary Antibodies Beta-2-Microglobulin Beta-Catenin
Polyclonal Clone 17C2 1 mL lyophilized NCL-B2Mp P (Enzyme) O 1 mL, 0.1 mL lyophilized NCL-B-CAT F P (HIER) W Beta-2-microglobulin, a single polypeptide chain of molecular weight 11.6 7 mL Bond ready-to-use PA0083 P (HIER) New! kD, is present on the surface of most nucleated cells and its expression may The catenins, (alpha, beta and gamma) are cytoplasmic proteins which bind be decreased or lost in malignancy. Beta-2-microglobulin is the major to the highly conserved tail of the E-cadherin molecule. Beta-catenin is a constituent of a subtype of secondary amyloidosis which is associated with component of the adherens junction, a multiprotein complex which supports long term hemodialysis. Clinical and pathological features of this disease Ca2+ -dependent cell to cell contact which in itself is critical for adhesion, have been characterized. Spontaneous fractures and destructive arthro- signal transmission and for anchoring the actin cytoskeleton. Beta-catenin’s pathies (articular swelling and pain in an oligoarticular distribution, along role is as a transcription effector of the wnt-signalling pathway. Immuno- with effusions in large joints) have been related to amyloid deposition. histochemistry is the best way to demonstrate nuclear expression of beta- Amyloid has been implicated in most clinical complaints of beta-2- catenin and wnt-pathway activation. This aberrant expression is observed in microglobulin-related amyloid arthropathy where it is found in synovial human tumorigenesis and especially in colorectal cancer. biopsies taken from the involved joints. Refer to page 15 for the Bond ready-to-use format. Product Specific Information NCL-B2Mp is also effective in ELISA techniques.
Beta Amyloid
Clone 6F/3D 1 mL lyophilized NCL-B-Amyloid FP Beta amyloid is an extracellular filamentous protein deposit found in the brain. It is the major protein component of amyloid cores and neuritic Human endometrial adenocarcinoma: immunohistochemical staining for beta-catenin using plaques and is also found as a deposit in neurofibrillary tangles. In man, NCL-B-CAT. Note membrane staining of tumor cells. Paraffin section. Primary Antibodies Alzheimer's disease is the most common cause of senile dementia and is characterized by abnormal filamentous protein deposits in the brain. Beta amyloid deposits are also detected in Lewy body dementia, Down's syndrome, amyloidosis (Dutch type) and in the Guam Parkinson-Dementia complex. Beta-Dystroglycan Product Specific Information Pretreatment of tissue sections with 98 to 100 percent formic acid is Clone 43DAG1/8D5 recommended when using NCL-B-Amyloid. 1 mL, 0.1 mL lyophilized NCL-b-DG FWE Dystrophin associated glycoproteins (DAGs) are a complex of at least seven proteins involved in the attachment of dystrophin to muscle membranes. The biological significance of this dystrophin/glycoprotein complex is not fully understood, but it appears to form an essential linkage between actin on the inside of the muscle fiber and muscle laminin in the basal lamina which surrounds the fiber. Beta-dystroglycan spans the muscle membrane and it has been suggested that it is the member of the complex which binds directly to dystrophin. Labeling of beta-dystroglycan may be reduced in some forms of muscular dystrophy where another component eg dystrophin or laminin, is directly affected. Labeling with an antibody to beta-spectrin to monitor membrane integrity, is an essential immunohistochemical control.
Human brain, Alzheimer's disease: immunohistochemical staining for beta amyloid protein using NCL-B-Amyloid. Note intense staining of senile plaques. Paraffin section.
Western blot: detection of human beta-dystroglycan (43 kD) using NCL-b-DG. Lane A, molecular weight markers. Lane B, human skeletal muscle extract immunoblotted with NCL-b-DG.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. ua yli el.NLB2i sflfrtedtcino myeloid of detection the for of useful forms is mature sarcomas. and NCL-BM2 granulocytic and precursor leukemias cells. early myeloid with react human NCL-BM2 and NCL-BM1 of human identification in Information the Specific in Product expressed useful antigen are differentiation. cellular cytoplasmic cells of levels myeloid different a of with Markers granulocytes. reactive is NCL-BM2 NCL-BM2 lyophilized mL 1 BM-2 Clone Marker) (Myeloid BM2 leukemia myelogenous acute M3 and M2 to reactivity subtypes. its (AML) to malignant appears in and BM-1 normal restricted designated antigen is of be the nucleus protein markers, and other DNA-binding cytoplasm Unlike cells. the The protein myeloid in cells. found DNA-binding be precursor myeloid-specific to myeloid kD reported human 183 in a with expressed reactive is NCL-BM1 NCL-BM1 lyophilized mL 1 BM-1 Clone Marker) (Myeloid BM1 109. page on XIIIa) Factor Coagulation (Blood XIIIa Factor also See PA0449 ready-to-use Bond mL 7 NCL-FXIIIa lyophilized mL 0.1 mL, 1 E980.1 Clone XIIIa) (Factor XIIIa Factor Coagulation Blood 75. page on (BL-CAM) CD22 also See NCL-CD22-2 lyophilized mL 0.1 mL, 1 FPC1 Clone (CD22) BL-CAM (HIER) P F FP (HIER) P (HIER) P (HIER) P loepesdi ohbng n rsai yepai n ocular and hyperplasia prostatic also and are 5 benign to 6 1 both to BMPs with in melanoma. 1 adenocarcinomas expressed prostatic BMPs ossificans in also lesions. expressed fibrodysplasia lymphoblastoid be fibroproliferative in to in overexpressed reported preosseous implicated uniquely and be is to cells reported BMP4 is worms. It found system, pathway progressive. and signalling nervous a insects peripheral in participates in and also a It central skeleton. embryonic in and to in developing musculature molecule formation the signalling binds a bone including as that stimulates roles tissues important it dimer has because it a mammals, activity. discovered adult is kinase was serine/threonine BMP4 BMP4 with Although Mature super- receptor related (TGF-beta) proteins. transmembrane structurally beta multimeric secreted factor nine growth of of transforming one family the is to (BMP4) belonging 4 BMPs protein morphogenetic Bone NCL-BMP4 lyophilized mL 0.1 mL, 1 3H2 Clone 4 Protein Morphogenetic Bone ula tiigo ml rprino rp pteilcls aafnsection. Paraffin cells. epithelial crypt intense of Note proportion NCL-BrdU. small using a stained of and staining BrdU nuclear with incubated biopsy colon human Normal via NCL-BrdU incubation antibody finally and embedding and sectioning immunohistochemistry. fixation by before fresh incubate bromodeoxyuridine followed to with requirement fragments a indicates tissue mitogenic product to with tumors. cells on included treatments of incorporation *Protocol various response of BrdU effects proliferative of the the monitor detection to determine The and stimuli to DNA. used their detects be into and can thymidine proliferation BrdU of incorporated cell have place which measuring in species animal for different method from for cells accurate staining nucleated be Immunocytochemical an division. can is cell the to BrdU BrdU into prior BrdU phase, thymidine. incorporate S turn during in of DNA which cells analogue proliferating *live an to introduced is (BrdU) 5-Bromodeoxyuridine NCL-BrdU lyophilized mL 1 85-2C8 Clone Bromodeoxyuridine NCL-BMP4. with immunoblotted A, line Lane cell NCL-BMP4. Saos-2 using B, kD) Lane (21 markers. 4 weight protein molecular morphogenetic bone of detection blot: Western W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence FP PW O E te applications Other lcrnmicroscopy Electron /63
Primary Antibodies CA19-9 (Sialyl Lewisa )
Clone C241:5:1:4 1 mL lyophilized NCL-CA19-9 F P (HIER) 1 mL liquid NCL-L-CA19-9 F P (HIER) 7 mL Bond ready-to-use PA0424 P (HIER) CA19-9 is an epitope on the sialylated Lewisa carbohydrate structure. Sialylated Lewisa plays a role in cell adhesion by acting as a functional ligand for the inducible adhesion molecule E-selectin. CA19-9 and CA50 (carcinoma associated mucin antigen) are useful serum markers in the Papillary carcinoma of endometrium: immunohistochemical staining for ovarian cancer diagnosis and follow up of gastrointestinal and pancreatic cancers. In antigen using NCL-L-CA125. Note staining of the luminal surface of malignant endometrial carcinoma of the pancreas, it is reported that the immunohistochemical cells. Paraffin section. expression of both CA19-9 and CA50 correlates with tumor differentiation where the strongest staining is observed in well differentiated tumors. These two markers are also reported in a number of benign lesions such as chronic pancreatitis. Product Specific Information Calbindin Clone C241:5:1:4 reacts specifically with Sialyl Lewisa - containing glycolipids, showing no crossreaction with Lewisa, Lewisb, or other structurally related Clone KR6 molecules. The epitope recognized by NCL-L-CA19-9 is designated CA19-9 1 mL lyophilized NCL-CALBINDIN P (HIER) and is similar to CA50 (carcinoma associated mucin antigen). Calbindin is a calcium-binding protein belonging to the troponin C Refer to page 16 for the Bond ready-to-use format. superfamily. It functions as a buffer of cytosolic calcium and is found in the brain, kidney, gut and pancreatic islets. In normal brain, calbindin (28 kD) has been identified in medium sized neurons of the neuropil of the matrix compartment of the striatum, the woolly fiber arrangements of the globus pallidus and the fiber structures of the pars reticula of the substantia nigra. The normal expression of calbindin is modified in patients with progressive supranuclear palsy, striatal degeneration and Huntingdon's disease (HD). In Primary Antibodies HD, alterations to the dendritic arbors and spiny striatal neurons may be visualized by immunohistochemistry for calbindin. In moderate grades of HD, proliferative changes have been found in these areas and in severe grades, degenerative changes have been noted. A proportion of dendritic cells within the light zone of germinal centers are also noted to be positive for calbindin.
Normal human colon: immunohistochemical staining for Sialyl Lewisa antigen using Human brain, cerebellum: immunohistochemical staining for calbindin using NCL-CALBINDIN. NCL-L-CA19-9. Note extracellular-associated staining of colonic epithelial cells. Paraffin Note cytoplasmic staining of Purkinje cells and neuronal processes. Paraffin section. section.
CA125 (Ovarian Cancer Antigen)
Clone Ov185:1 1 mL lyophilized NCL-CA125 F P (HIER) 1 mL liquid NCL-L-CA125 F P (HIER) 7 mL ready-to-use RTU-CA125 F P (HIER) 7 mL Bond ready-to-use PA0539 P (HIER) CA125 antigen is usually associated with ovarian epithelial malignancies. Serum assays are widely used to detect this protein in the monitoring of ovarian cancers. CA125 antigen may also be detected by immuno- histochemistry and expression has been found in neoplasms such as seminal vesicle carcinoma and anaplastic lymphoma. CA125 antigen is not found exclusively in malignant tumors. CA125 is also known as MUC16. Refer to page 16 for the Bond ready-to-use format.
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L LlohlzdNCL-CALP-12A2 lyophilized mL 1 mL, 2.5 Calp3c/12A2 Clone NCL-CALP-11B3 lyophilized 1mL mL, 2.5 Calp3c/11B3 Clone NCL-CALP-2C4 lyophilized mL 2.5 Calp3d/2C4 Clone Calpain Paraffin cells. malignant of using section. staining calmodulin membrane for and staining cytoplasmic immunohistochemical Note breast: NCL-CALMODULIN. of carcinoma ductal situ in Human api ad t9 n 0k,pe n otatlzdfrso the of forms post-autolyzed and pre- kD, ( with 60 2 reacts and and NCL-CALP-11B3 1 94 these calpains mutations. at all ubiquitous gene of bands null loss 3 kD. the with calpain 60 by samples confirmed approximately been in at has bands products antibodies these degradation of full- apparent additional Specificity with an plus reacts and NCL-CALP-12A2 protein muscle. kD) skeletal size (94 human 3 in kD) calpain (30 full-size homogenates fragment the with in reacts NCL-CALP-2C4 and blot. (1998)), Western Information by Specific analysis Product 1169-1179 for suited well 153(4): therefore is American in and al. Pathology. 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Primary Antibodies Calretinin (5A5)
Clone 5A5 1 mL, 0.1 mL lyophilized NCL-CALRETININ P (HIER) 1 mL liquid NCL-L-CALRETININ P (HIER) 7 mL ready-to-use RTU-CALRETININ P (HIER) Calretinin is an intracellular calcium-binding protein of 31.5 kD belonging to the troponin C superfamily characterized by a structural motif described as the EF-hand domain. Calcium is an important moderator of a number of vital physiological processes, including neuronal excitability, axonal transport, Western blot: analysis of human skeletal muscle showing detection of the calpain family of proteins. Lane A, calpain 3 bands at 94 and 30 kD detected with NCL-CALP-2C4. Lane B, synthesis and release of some neurotransmitters, membrane permeability calpains 1, 2 and 3 detected with NCL-CALP-11B3. Lane C, calpain 3 bands at 94 and and enzyme activity. Calretinin is found in the nervous system and thymus. approximately 60 kD detected with NCL-CALP-12A2. Photograph supplied courtesy of Dr Louise Calretinin can also be demonstrated in normal and neoplastic mesothelial V B Anderson. cells and has been reported to be a useful marker for the identification of malignant mesotheliomas of epithelial type to differentiate these from metastases of lung adenocarcinoma where antibodies to detect calretinin are used in within a panel and interpretation together with clinical data is Calponin (Basic) undertaken by a qualified pathologist.
Clone 26A11 1 mL, 0.1 mL lyophilized NCL-CALPONIN-B F P (HIER) W 7 mL Bond ready-to-use PA0416 P (HIER) Basic calponin (calponin-h1) is a 34 kD protein which exhibits a high degree of homology to acidic and neutral calponins at its N-terminal region. It is an actin, tropomyosin and calmodulin binding protein thought to be involved in the regulation of smooth muscle contraction. The expression of basic calponin is reported to be restricted to smooth muscle cells and is a marker Primary Antibodies of the differentiated contractile phenotype of developing smooth muscle. Vascular smooth muscle cells convert to a synthetic dedifferentiated Human mesothelioma: immunohistochemical staining for calretinin using NCL-L-CALRETININ. phenotype when this protein is lost and this is a key stage in both Note cytoplasmic staining of malignant cells. Paraffin section. atherosclerosis and restenosis of coronary arteries after balloon angioplasty. It is thought that basic calponin exerts its effect via the cortical actin cytoskeleton and therefore influences proliferation, the transformed phenotype and the metastatic potential of tumor cells. Basic calponin mRNA is expressed in smooth muscle of prostate, bowel and aorta whereas neutral Calretinin (CAL6) and acidic calponin mRNAs are expressed in non-smooth muscle tissues such as heart, placenta, lung, kidney, pancreas, spleen, testis and ovary as Clone CAL6 well as in smooth muscle-containing tissues. 1 mL liquid NCL-L-CALRET-566 P (HIER) W Reference Range Refer to page 16 for the Bond ready-to-use format. 7 mL Bond ready-to-use PA0346 P (HIER) Calretinin is a calcium-binding protein of 29 kD that is a member of the family of so-called EF-hand proteins that also includes S-100 proteins. Calretinin is reported to be abundantly expressed in neurons. Outside the nervous system, calretinin is reported to be expressed in a range of cell types including mesothelial cells, steroid producing cell, (eg adrenal cortical cells, Leydig cells, ovarian theca interna cells as well as Sertoli cells, some neuroendocrine cells, eccrine sweat glands) and other cell types. The presence of calretinin is reported to be a useful marker primarily for differentiating malignant mesothelioma from carcinomas. Other reports indicate the detection of calretinin is helpful for the differential characterization of ovarian stroma tumors. Calretinin-positive cells have also been reported in the convoluted tubules of kidney with some Normal human appendix: immunohistochemical staining for basic calponin using NCL-CALPONIN-B. Note cytoplasmic staining of smooth muscle. Paraffin section. antibodies. Refer to page 17 for the Bond ready-to-use format.
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Primary Antibodies Caspase-8 Cathepsin B
Clone 11B6 Clone CB131 1 mL, 0.1 mL lyophilized NCL-CASP-8 F P (HIER) 1 mL lyophilized NCL-CATH-B P The caspases represent a family of cysteine proteases that play important Cathepsin B is one member of a family of proteolytic enzymes and is regulatory roles within the cell. Caspase-8, also called FLICE, has an N- expressed in cytoplasmic lysosomes in different types of normal and terminal domain with sequence homology to the death effector domain of neoplastic tissues. It is a cysteine protease and like most cathepsins is FADD that allows association of caspase-8 with the TNF/Fas family of involved in cellular metabolism such as protein degradation. receptors. This association with the cell surface death receptors has shown Immunohistochemical studies have detected expression in bowel mucosa, caspase-8 to be a proximal regulator of apoptosis. Caspase-8 is activated by skin, prostate and thyroid. Staining for cathepsin B, in common with other association with the Fas/FADD death-inducing signalling complex to release cathepsins, may be so intense that it appears to be nuclear in some cells. A two active subunits, p18 and p10, into the cytosol, where they activate other proportion of endothelial cells are positive in many tissues. This has been caspases amplifying the apoptotic signal. Caspase-8 is reported to be reported previously where it has been described as sprouting endothelial expressed in pancreatic tumors, high grade non-Hodgkin's lymphomas and cells. In tissues containing tumors this is thought to be related to tumor invasive breast carcinomas. progression. Cathepsin B is an important matrix-degrading protease in several human cancers including lung adenocarcinomas, squamous cell Product Specific Information carcinomas, rectal and breast carcinomas. Cathepsin B is reported to be NCL-CASP-8 is raised to the p18 subunit found in caspases 8a, 8b and 8h. overexpressed in squamous cell carcinoma where undifferentiated cells are strongly positive and the more differentiated cells in tumor islands are either weakly positive or negative. The expression of cathepsin B has also been reported in melanomas where the upregulation of this enzyme was found to Caspase-9 be a characteristic of a more invasive tumor phenotype.
Clone 2C9B11 1 mL, 0.1 mL lyophilized NCL-CASP-9 P (HIER) W Caspase-9 is a member of the caspase family of cysteine proteases that has been implicated in apoptosis and cytokine processing. Caspases have been shown to be activated during normal human keratinocyte differentiation and Primary Antibodies this activation is required for the normal loss of the nucleus. In addition, this apoptotic pathway may be activated in cardiac myocytes under conditions of ischemia. In the presence of ATP, apoptotic stimuli induce proteolytic processing and activation of pro-caspase 9 by cytochrome c and Apaf-1. Activated caspase-9 cleaves downstream caspases such as caspase-3, 6 Human skin: immunohistochemical staining for cathepsin B using NCL-CATH-B. Note intense and 7 initiating the caspase cascade. Caspase-9 is essential for apoptosis cytoplasmic staining of basal epithelium and reduced staining in suprabasal cells. Paraffin during the normal development of the central nervous system. Mutations or section. deficiencies in caspase-9 result in resistance to apoptotic stimuli that mimic conditions in developing tumors. Cathepsin D
Clone C5 1 mL, 0.1 mL lyophilized NCL-CDm FP Cathepsins are members of the papain family of cysteine lysosomal proteases which are involved in a variety of physiological processes such as proenzyme activation, enzyme inactivation, antigen presentation, hormone maturation, tissue remodelling and bone matrix resorption. Cathepsin D is first produced in a precursor form, pro-cathepsin D (52 kD), Human rectal adenocarcinoma: immunohistochemical staining for caspase-9 protein using and then processed in the cell to an intermediate form of 48 kD, then finally NCL-CASP-9. Note cytoplasmic staining of neoplastic epithelium. Paraffin section. to the mature forms of 34 kD and 14 kD. It has been proposed that the presence of high levels of cathepsin D in breast cancer may signify a functional estrogen receptor apparatus.
Human breast carcinoma: immunohistochemical staining for cathepsin D using NCL-CDm. Note granular cytoplasmic staining of tumor cells. Paraffin section.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. yolsi tiigo rprino eaoye.Prfi section. Paraffin hepatocytes. of granular proportion Note a NCL-CATH-L. of using staining L cytoplasmic cathepsin for staining immunohistochemical liver: Human respective their through matrix to ability extracellular as their by well activities. surrounding invasion proteolytic as cancer the (PAI-1) in role cancers. inhibitor important destroy protease thyroid an play (uPA), and proteases protease prostate other serine bladder, L, in adrenal, expressed Cathepsin in levels colonic, normal found above ovarian, be in and detected lung to breast, be the to are of reported carcinomas L levels cell high Cathepsin non-small very of with tumors, levels occurs testicular observed and expression pathological kidney increased highest general, The of in but, cancers. is number L types in Cathepsin myocardial cell proteinuria. a normal to in all response in in and tubular present renal myopathies the implicated in in also been and necrosis ischemia has myofibrial inactivate L including can processes Cathepsin It activity vitro. vitro. elastase in neutrophil human in cathepsins controls in collagenolytic the which role potent of inhibitor protease major any most alpha-1 a of the activity plays exhibits elastinolytic which It and protease catabolism. cysteine protein lysosomal intracellular a is L Cathepsin NCL-CATH-L lyophilized mL 1 13C2 Clone L Cathepsin section. Paraffin leukocytes. intense polymorphonuclear Note of NCL-CATH-G. staining using G membrane cathepsin for staining immunohistochemical tonsil: Human chronic or lymphoblastic protein. this acute for acute negative whereas in are expressed leukemias leukemias be lymphocytic to myeloid reported chronic killing also is the and at G remodelling in Cathepsin tissue inflammation. connective participate of in may sites and proteases; pathogens proteases serine engulfed of three other digestion These and two contain hepsin. with which and together leukocytes elastase granules polymorphonuclear been neutrophilic azurophil in specialized Cathepsin have granulocytes, neutrophils. located from to phagocytes is proteases restricted G internalize mononuclear and is bind to However, tissues demonstrated normal neutrophils. in especially expression G Cathepsin NCL-CATH-G lyophilized mL 1 19C3 Clone G Cathepsin HE)W (HIER) P P oectpamcsann fsot uceadedteim aafnsection. Paraffin endothelium. and muscle smooth of NCL-L-Caveolin-1. staining using cytoplasmic caveolin-1 Note for staining immunohistochemical colon: human Normal sarcomas. as act such to malignancies likely human is some caveolin-1 in that suppressor tumor cancer, reported cytoplasm a have and as pancreatic and membrane studies cell Further In membranous the cells. on cancer granular described. expressed of be fine is to reported a pattern is caveolin-1 where staining levels fibroblasts cytoplasmic High in cells. diffuse endothelial reported and also adipocytes cells cells, be are mesenchymal muscle to It smooth differentiated reported is as types. terminally and such in cell pathways expressed different signalling abundantly several of in most membrane role regulatory plasma a vesicular the are plays which on caveolae present of component invaginations structural major a is Caveolin-1 NCL-L-Caveolin-1 liquid mL 0.1 mL, 1 4D6 Clone Caveolin-1 umcslgnlaa ela nedcieclso h gastrointestinal and the myenteric of the cells in endocrine fibers in as nerve well peripheral tract. as hypothalamus. in ganglia the mainly submucosal the and localized including nuclei brain is the amygdaloid CCK of hippocampus, regions several cortex, excitatory the in and cerebral distributed modulates GABA is 5-HT, and CCK dopamine, neurotransmitter acids. eg amino a neurotransmitters as other serves of gastrin-induced action also inhibiting CCK as well gall secretion. pancreas, as acid hormone the motility from intestinal gastrointestinal secretion contraction, enzyme the bladder stimulate to to functions belong CCK family. and polypeptide secretin gastrin, intestinal (CCK-8) CCK, octapeptide species. vasoactive C-terminal molecular exists the abundant CCK which most intestine, of brain the forms and represents in molecular brain of receptors the number both distinct a In in to membranes. reversibly cell and pancreatic peptide and saturably intestinal acid binds amino 33 that a hormone as isolated first was (CCK) Cholecystokinin NCL-CCK-8p lyophilized mL 0.25 Polyclonal (Cholecystokinin) CCK-8 W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence (Enzyme) P (HIER) P O E te applications Other lcrnmicroscopy Electron /69
Primary Antibodies CD1a CD2 (LFA-2)
Clone MTB1 Clone AB75 1 mL, 0.1 mL lyophilized NCL-CD1a-235 F P (HIER) 1 mL, 0.1 mL lyophilized NCL-CD2-271 P (HIER) 1 mL liquid NCL-L-CD1a-235 F P (HIER) Reference Range 1 mL liquid NCL-L-CD2-271 P (HIER) 7 mL ready-to-use RTU-CD1a-235 F P (HIER) 7 mL ready-to-use RTU-CD2-271 P (HIER) 7 mL Bond ready-to-use PA0235 P (HIER) Clone 11F11 Clone JPM30 7 mL Bond ready-to-use PA0271 P (HIER) 1 mL, 0.1 mL lyophilized NCL-CD1a-220 F P (HIER) The CD2 antigen (LFA-2) is a monomeric 45 to 58 kD glycoprotein. It is an CD1a is a protein of 43 to 49 kD expressed on dendritic cells and cortical accessory molecule important in mediating the adhesion of activated T cells thymocytes. CD1a antigen expression has been shown to be useful in and thymocytes with antigen-presenting cells and target cells. differentiating Langerhans cells, powerful antigen presenting cells present Refer to page 17 for the Bond ready-to-use format. in skin and epithelia, from interdigitating cells. Immunohistochemical studies for CD1a antigen have reported a reduction in epidermal Langerhans cells in graft versus host disease and the participation of CD1a antigen-positive dendritic cells in atherosclerotic lesion formation and asthmatic inflammation. Product Specific Information Clone MTB1 detects cortical thymocytes, Langerhans cells in epidermis, interdigitating cells of dermis and interdigitating cells of stratified squamous epithelium of tonsil. Clone MTB1 may also detect small focal groups of lymphocytes outside the germinal centers of tonsil indicating a cross- reaction with CD1b antigen. Clone JPM30 detects cortical thymocytes, Langerhans cells in epidermis, interdigitating cells of dermis, interdigitating cells of stratified squamous epithelium of tonsil but in addition it stains sweat Human small intestine, T cell lymphoma: immunohistochemical staining for CD2 antigen (LFA-2) gland ducts in the dermis and epithelial cells of small intestine indicative of using NCL-CD2-271. Note intense membrane staining of T lymphocytes. Paraffin section.
Primary Antibodies cross-reactivity with CD1d antigen. Refer to page 17 for the Bond ready-to-use format. CD3
Clone LN10 1 mL, 0.1 mL liquid NCL-L-CD3-565 P (HIER) Reference Range 7 mL Bond ready-to-use PA0553 P (HIER) Clone PS1 1 mL, 0.1 mL lyophilized NCL-CD3-PS1 P (HIER) W 1 mL liquid NCL-L-CD3-PS1 P (HIER) W 7 mL ready-to-use RTU-CD3-PS1 P (HIER) Normal human skin: immunohistochemical staining for CD1a antigen using NCL-CD1a-235. Note intense membrane staining of Langerhans cells. Paraffin section. Clone UCHT1 1 mL lyophilized NCL-CD3 FC Clone LN10 was developed to produce superior staining with PBS based buffers compared to clone PS1 on paraffin sections. The CD3 molecule consists of five different polypeptide chains with molecular weights ranging from 16 to 28 kD. The CD3 antigen is first detected in early thymocytes and its appearance probably represents one of the earliest signs of commitment to the T cell lineage. Product Specific Information Clone PS1 is specific for the non-glycosylated epsilon chain of the human CD3 molecule (Chetty R and Gatter K. Journal of Pathology. 173: 303-307 (1994)). Clone LN10, our newest clone, is also specific for the non-glycosylated epsilon chain of the human CD3 molecule. Clones LN10, PS1, and UCHT1 recognize T cells in thymus, bone marrow, peripheral lymphoid tissue and blood and are all pan T cell markers. Refer to page 18 for the Bond ready-to-use format.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. ee opg 8frteBn ed-oueformat. ready-to-use Bond the for 18 page to Refer reduced. be may intensity D-F.Nt ebaesann fiflrtn ypoye.Prfi section. Paraffin lymphocytes. T infiltrating NCL- of using staining antigen membrane CD4 Note for CD4-1F6. staining immunohistochemical fungoides: mycosis skin, Human H greater or percent 1 of use cell the that T note cutaneous Please of positive. Information cases CD4 Specific generally Product also Most is leukemia/lymphoma monocytes. HTLV- cell and T on adult antigen associated CD4 1 the level express fungoides, lower lymphocytes mycosis subset including blood cell lymphoma, a T peripheral a at of on expressed percent and is 45 antigen a CD4 representing with The glycoprotein (helper/inducer) kD. transmembrane where 59 chain of single weight a protocol molecular is (T4) molecule conventional CD4 to The allow incubation sections. antibody paraffin primary to on staining before superior produce blocked developed is peroxidase was endogenous 4B12 Clone RTU-CD4-1F6 ready-to-use mL 7 NCL-L-CD4-1F6 liquid mL 1 NCL-CD4-1F6 lyophilized mL 0.1 mL, 1 1F6 Clone PA0368 ready-to-use Bond mL 7 NCL-L-CD4-368 liquid mL 1 NCL-CD4-368 lyophilized mL 0.1 mL, 1 4B12 Clone CD4 section. Paraffin lymphocytes. T of NCL-L-CD3-565. staining using membrane antigen intense CD3 Note for staining immunohistochemical tonsil: human Normal erea ih05pretH before percent blocked 0.5 1F6. is with clone peroxidase retrieval by endogenous that recognized recommended epitope is the it on Therefore, effect detrimental a has peroxidase 2 O 2 mtao o 0mnts tews staining otherwise minutes, 10 for /methanol HE)W (HIER) P (HIER) P F (HIER) P (HIER) P HE)W (HIER) P HE)W (HIER) P F 2 O 2 obokendogenous block to ee opg 8frteBn ed-oueformat. antigen ready-to-use Bond the CD5 cell for T 18 page and nodes. to B Refer as lymph well (T-ALL), as leukemias cell normal (B-CLL) lymphocytic leukemias T acute lymphocytic lymphomas. of cell chronic many cell T zones by B in some located reported expressed mantle cells also B also is of the is expression subset a in antigen on and CD5 primarily cells T cells. activated T lymphomas, leukemias, reactivity on main 72 the and observed nodes, thymocytes lymph of is In percent lymphocytes. 95 blood on peripheral expressed of be percent to reported is antigen CD5 PA0168 ready-to-use Bond mL 7 RTU-CD5-4C7 ready-to-use mL 7 NCL-L-CD5-4C7 liquid mL 1 NCL-CD5-4C7 lyophilized mL 0.1 mL, 1 4C7 Clone CD5 1F6. clone by recognized epitope the 1F6 on effect clone detrimental a has that peroxidase note Please antigens. monoclonal H CD8 supplies percent and which 0.5 pack CD4 duo requires both the to with (CD8 identified cells antibodies T be cytotoxic/suppressor can and positive) positive) (CD4 cells T pack. Helper/inducer one in antibodies two offer Microsystems Leica convenience, NCL-CD4/CD8d For lyophilized mL 0.5 × 2 4B11 Clone and 1F6 Clone pack) (duo Antibodies CD8 and CD4 C-D-C.Nt nes ebaesann ftmrcls aafnsection. Paraffin cells. tumor of staining using membrane antigen intense CD5 Note for NCL-CD5-4C7. staining immunohistochemical lymphoma: cell mantle Human lont htteueo ecn rgetrH greater or percent 1 of use Please the intensity. staining that in note reduction any also prevent to EDTA using unmasking W P F etr blotting Western Paraffin Frozen 2 O 2 C I mtao ramn o 0mntsBEFORE minutes 10 for treatment /methanol lwcytometry Flow Immunofluorescence HE)W (HIER) P (HIER) P (HIER) P HE)W (HIER) P HE)W (HIER) P 2 Reference Range O 2 obokendogenous block to O E te applications Other lcrnmicroscopy Electron /71
Primary Antibodies CD7
Clone LP15 1 mL, 0.1 mL liquid NCL-L-CD7-580 P (HIER) Reference Range 7 mL Bond ready-to-use PA0266 P (HIER) The CD7 molecule is a membrane-bound glycoprotein of 40 kD and is the earliest T cell specific antigen to be expressed in lymphocytes. CD7 antigen is also the only early marker to persist throughout differentiation. The function and role of the CD7 molecule has not yet been fully identified, although the activation of T cells with gamma/delta receptors has been proposed based on mAb-induced activation. CD7 antigen is reported to be Large T cell immunoblastic lymphoma: immunohistochemical staining for CD8 antigen using found on the majority of peripheral blood T cells, most natural killer cells and NCL-CD8-295. Note intense membrane staining of T lymphocytes. Paraffin section. thymocytes. Product Specific Information Clone LP15 was developed to provide superior staining to clone CD7-272 on paraffin sections. CD9 (Motility-Related Protein-1) Refer to page 18 for the Bond ready-to-use format. Clone 72F6 1 mL lyophilized NCL-CD9 F P (HIER) CD9 antigen is a 24 to 27 kD glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophils, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. It belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre-B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41/CD61 (GPIIb/GPIIIa). It also regulates cell motility in a variety of cell lines and appears to be an
Primary Antibodies important regulator of Schwann cell behavior in the peripheral nervous system. In melanoma and breast cancer, CD9 antigen expression has been Human thymus: immunohistochemical staining for CD7 antigen using NCL-L-CD7-580. reported to occur predominantly on primary, non-metastatic tumors. Note intense staining of cortical thymocytes. Paraffin section.
CD8
Clone 1A5 1 mL, 0.1 mL lyophilized NCL-CD8-295 F P (HIER) W 1 mL liquid NCL-L-CD8-295 F P (HIER) W Reference Range 7 mL ready-to-use RTU-CD8-295 F P (HIER) Human fibroadenoma: immunohistochemical staining for CD9 antigen using NCL-CD9. Clone 4B11 Note intense membrane staining of tumor cells. Paraffin section. 1 mL, 0.1 mL lyophilized NCL-CD8-4B11 F P (HIER) W 7 mL Bond ready-to-use PA0183 P (HIER) The CD8 molecule is composed of two chains and has a molecular weight of 32 kD. It is found on a T cell subset of normal cytotoxic/suppressor cells which make up approximately 20 to 35 percent of human peripheral blood lymphocytes. The CD8 antigen is reported to be detected on natural killer cells, 80 percent of thymocytes, on a subpopulation of 30 percent of peripheral blood null cells and 15 to 30 percent of bone marrow cells. Refer to page 19 for the Bond ready-to-use format.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. eacanC1 9 D.Teapacancmoet(D1)o the of (CD11b) monocytes, component granulocytes, chain on alpha expressed cells. The NK is and macrophages kD). protein a (95 associated common to a belongs leukocyte CD18 share It which chain fragment. CD11) complement (designated poly- beta C3bi proteins two heterodimeric the as of for of functions family receptor Mac-1 kD. consists surface 170 and cell protein kD a 95 associated weights molecular with leukocyte chains peptide (Mac-1) CD11b The NCL-CD11b lyophilized mL 1 44 Clone (Mac-1) CD11b section. Paraffin cells. lymphoid neoplastic of staining membrane using intense antigen Note CD10 NCL-CD10-270. for staining immunohistochemical lymphoma: lymphoblastic Human 77-82 154(1): format. ready-to-use Bond Pathology. the for of 19 page Journal to Refer American epithelial al. gut and et within and kidney (1999)). McIntosh of cells cells border with (G. non-lymphoid brush B fibroblasts, the cells. canaliculi, various on center bile expression in cells, germinal high myoepithelial expressed especially and breast also as marrow such is tissues, bone It adult tissue. lymphoid within immature cells, been progenitor cells other has lymphoid early In antigen B normal tumor-specific. of cells. CD10 surface neoplastic the lymphomas. of on be and cells follicular identified surface on normal and expressed be to of Burkitt's to cell variety reported lymphoblastic, is wide thought antigen expressed CD10 a kD is malignancies, of lymphoblastic lymphoid antigen was surface CD10 acute 100 that the and common shown on have a the however, (CALLA) active studies, as Subsequent is biologically identified antigen of initially neprilysin, leukemia variety was It a called peptides. inactivates also which metalloendopeptidase antigen, CD10 PA0270 ready-to-use Bond mL 7 RTU-CD10-270 ready-to-use mL 7 NCL-L-CD10-270 liquid mL 1 NCL-CD10-270 lyophilized mL 0.1 mL, 1 56C6 Clone CD10 HE)W (HIER) P F F (HIER) P (HIER) P F HE)W (HIER) P F Reference Range nie xrsini eotdt eaueu niao fmonocyte format. ready-to-use of Bond the indicator for 19 the page for useful to Refer and a leukemias myeloid leukemias. be cell acute hairy of of to identification classification the reported including in lines, is differentiation CD11c cells. cell dendritic expression lymphoid interdigitating of cells, antigen proportion T a to and activated reported leukemias also cells, cell is hairy NK It on granulocytes. in expressed levels be lowest in macrophages with tissue and eg on monocytes reported cells and levels myeloid high with on neutrophils non-segmented segmented mainly metamyelocytes, and tissues, premyelocytes, is normal myelocytes, It marrow in proteins. bone adhesion expressed of be family integrin to leukocyte reported the of member a is CD11c PA0554 ready-to-use Bond mL 7 NCL-L-CD11c-563 liquid mL 0.1 mL, 1 5D11 Clone CD11c el,fbolss ri el,bn arw secat n el iigthe lining cells and osteoclasts endothelial marrow, canaliculi. border, bone bile brush cells, include brain intestinal fibroblasts, antigen tubules, cells, lymphoid proximal CD13 acute renal express of cells, which proportion epithelial smaller cells a is Non-hematopoietic and antigen precursors, leukemias. leukemias their CD13 and myeloid N- peptides. monocytes acute the granulocytes, II-bound most on trimming class expressed by be MHC presentation to from metalloprotease reported antigen surface acids zinc-binding several cell amino a the in and terminal as role for humans a functions receptor plays in antigen a which disease is The antigen species. respiratory leukocyte CD13 animal cause BP-1. the which II and includes type CD73 coronaviruses the also CD26, of member CD10, which a antigens metalloproteases is N, membrane aminopeptidase as integral known also antigen, CD13 NCL-CD13-304 lyophilized mL 0.1 mL, 1 38C12 Clone CD13 section. Paraffin cells. malignant of using staining antigen membrane CD11c Note for NCL-L-CD11c-563. staining immunohistochemical leukemia: cell hairy Human W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence (HIER) P (HIER) P (HIER) P O E Reference Range te applications Other lcrnmicroscopy Electron /73
Primary Antibodies CD14
Clone 7 1 mL, 0.1 mL lyophilized NCL-CD14-223 P (HIER) W 1 mL liquid NCL-L-CD14-223 P (HIER) W CD14 antigen is a glycosyl-phosphatidylinositol (GPI)-linked glycoprotein with a molecular weight of 55 kD. The CD14 antigen is reported to be expressed on cells of the myelomonocytic lineage including monocytes, macrophages and Langerhans cells. Low expression is also reported on neutrophils and on B cells. CD14 antigen is a receptor for bacterial lipopolysaccharide (LPS, endotoxin) and the lipopolysaccharide binding Hodgkin's disease: immunohistochemical staining for CD15 antigen using NCL-CD15. Note protein (LBP). LBP and CD14 antigen serve two physiological roles. These membrane staining and characteristic staining of paranuclear hofs of Reed Sternberg cells. proteins act as opsonin and opsonic receptor, respectively, to promote the Paraffin section. phagocytic uptake of bacteria or LPS-coated particles by macrophages.
CD16
Clone 2H7 1 mL, 0.1 mL lyophilized NCL-CD16 P (HIER) CD16 antigen has a molecular weight of 50 to 70 kD and is a low affinity Fc receptor for complexed IgG, Fc/gamma RIII, expressed on natural killer (NK) cells, granulocytes, activated macrophages and a subset of T cells expres- sing alpha-beta or gamma-delta T cell antigen receptors. The CD16 antigen exists both as a glycosyl-phosphatidylinositol (GPI)-anchored protein in Human uterus: immunohistochemical staining for CD14 antigen using NCL-CD14-223. Note polymorphonuclear cells and as a transmembrane protein in NK cells. membrane staining of macrophages within an endometrial gland and lymphocytes in the stroma. Paraffin section. Primary Antibodies
CD15
Clone BY87 1 mL, 0.1 mL lyophilized NCL-CD15 F P (HIER/Enzyme) 1 mL liquid NCL-L-CD15 F P (HIER/Enzyme) Reference Range 7 mL ready-to-use RTU-CD15 F P (HIER/Enzyme) Human colon, ulcerative colitis: immunohistochemical staining for CD16 antigen using NCL-CD16. Note intense membrane staining of infiltrating natural killer cells, granulocytes Clone Carb-1 and activated macrophages. Paraffin section. 7 mL Bond ready-to-use PA0039 P (HIER) CD15 antigen, also known as X-hapten, is reported to be expressed on 90 percent of circulating human granulocytes, 30 to 60 percent of circulating monocytes and is absent from normal lymphocytes. The CD15 antigen is also CD19 expressed on Reed Sternberg cells of Hodgkin's disease and some leukemias. Clone BT51E Product Specific Information 1 mL, 0.1 mL liquid NCL-L-CD19-163 P (HIER) W Reference Range Please note that either enzyme pretreatment or heat induced epitope 7 mL Bond ready-to-use PA0843 P (HIER) retrieval (HIER) temperature antigen unmasking using 1mM EDTA (pH8.0) solution may be used on fixed, paraffin-embedded tissue. The choice of antigen unmasking technique to provide the best result should be determined Clone 4G7/2E by the user. 1 mL, 0.1 mL lyophilized NCL-CD19-2 FC Clone BT51E was developed to be effective on formalin-fixed, paraffin- Refer to page 19 for the Bond ready-to-use format. embedded tissue sections. CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. It is a single chain glycoprotein present on the surface of B lymphocytes and follicular dendritic cells of the hematopoietic system. CD19 is a crucial regulator in B cell development, activation and differentiation. On B cells, CD19 associates with CD21, CD81 and CD225 (Leu-13) forming a signal transduction complex. CD19 is expressed from the earliest recognizable B cell lineage stage, through development to B cell differentiation but is lost on maturation to plasma cells. Refer to page 20 for the Bond ready-to-use format.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. C-D071 oeitnemmrn tiigo el.Prfi section. Paraffin cells. B of staining membrane using intense antigen Note CD20 NCL-CD20-7D1. for staining immunohistochemical lymphoma: centroblastic Human format. ready-to-use Bond the for 20 from page to cells Refer B leukemias differentiation. normal acute and on marrow, leukemias. lymphocytic bone activation expressed chronic tonsil, is and be cell spleen, and node, to B lymph cells reported blood, during B peripheral been receptor human has malignant a antigen and as CD20 normal approximately act on of to expressed phosphoprotein thought is non-glycosylated which a kD is 33 antigen CD20 paraffin The on staining manual superior produce to sections. developed was 7D1 Clone RTU-CD20-L26 ready-to-use mL 7 NCL-L-CD20-L26 liquid mL 1 NCL-CD20-L26 lyophilized mL 1 L26 Clone PA0906 ready-to-use Bond mL NCL-CD20-MJ1 7 lyophilized mL 0.1 mL, 1 MJ1 Clone NCL-CD20-7D1 lyophilized mL 0.1 mL, 1 7D1 Clone CD20 section. Paraffin cells. Note B NCL-L-CD19-163. of using staining CD19 membrane for staining immunohistochemical marrow: bone Human Reference Range HE)W (HIER) P F HE)W (HIER) P F (HIER) P (HIER) P F HE)W (HIER) P F (HIER) P F LBn ed-ouePA0249 ready-to-use Bond mL 7 NCL-CD22-2 lyophilized mL 0.1 mL, 1 FPC1 Clone (BL-CAM) CD22 section. Paraffin cells. dendritic follicular of staining NCL-CD21- membrane using mature intense antigen Note CD21 2G9. for staining lymphomas, immunohistochemical lymphoma: follicular Human cell format. ready-to-use Bond mantle the for 20 and page to follicular Refer follicular in expressed in and be lymphomas. monocytes and and to leukemias lymphocytes, reported cells T also on is dendritic acute antigen absent cell CD21 T is of granulocytes. subset but a be in to leukemias and reported leukemias is lymphocytic lymphocytic antigen chronic CD21 cell virus. B Epstein-Barr cell in third for B found receptor the the is a of of cells, interior as the acts B fragment to and mature C3d signals on growth-promoting the transmitting present for in molecule, involved receptor CD21 the The component. as weight complement functions molecular of which glycoprotein membrane kD, integral 140 I type a is antigen CD21 PA0171 ready-to-use Bond mL 7 NCL-L-CD21-2G9 liquid mL 1 NCL-CD21-2G9 lyophilized mL 0.1 mL, 1 2G9 Clone CD21 ee opg 0frteBn ed-oueformat. ready-to-use Bond the for peripheral 20 on page to absent monocytes. Refer and is granulocytes It leukemias, leukemias. cell reported T cell cells, also hairy T is on blood antigen expressed CD22 strongly is differentiation. be expression upon antigen to lost Surface be expression. may same antigen and the CD19 variable approximately to the at of reported differentiation that is as lymphocyte antigen the stage cell CD22 in B the and in of early membrane Expression appear the independently lymphocytes. two on B of of both heterodimer a cytoplasm present is chains, It kD. glycoprotein with 140 glycoprotein to expressed membrane 130 integral of 1 weight type molecular a a is (BL-CAM) antigen CD22 The W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence (HIER) P F (HIER) P (HIER) P (HIER) P (HIER) P F O E te applications Other lcrnmicroscopy Electron /75
Primary Antibodies CD23 CD29
Clone 1B12 Clone 7F10 1 mL, 0.1 mL lyophilized NCL-CD23-1B12 F P (HIER) 1 mL, 0.1 mL lyophilized NCL-CD29 P (HIER) 1 mL liquid NCL-L-CD23-1B12 F P (HIER) C Reference Range The 1 integrins are a family of structurally-related heterodimeric molecules 7 mL ready-to-use RTU-CD23-1B12 F P (HIER) and are composed of a 1 subunit (CD29 antigen) which is associated with 1 7 mL Bond ready-to-use PA0169 P (HIER) of 6 known alpha subunits. These impart the specificity to each of the receptors and the VLA molecules which are designated according to their The CD23 molecule is the low affinity IgE receptor found on B cells. It is a alpha chain eg VLA-1 is 1/1, VLA-2 is 2/1. The adhesive properties of membrane glycoprotein of 45 kD and is reported to be found on a sub- CD29 heterodimers on T cells can be regulated by cell activation, possibly population of peripheral blood cells, B lymphocytes and on EBV-transformed through interactions between the cytoplasmic domain of CD29 antigen and B lymphoblastoid cell lines. Expression of CD23 antigen has been reported the cytoskeleton. CD29 antigen is reported to be expressed on most cells on monocytes and dendritic cells. including all leukocytes, although only at low levels on granulocytes. On T cells, CD29 antigen is expressed at higher levels on memory cells than on Refer to page 21 for the Bond ready-to-use format. naive cells. The co-expression of CD4 and CD29 antigens is found in helper/ inducer subpopulation of CD4 lymphocytes. CD29 antigen is one of several additional molecules reported to be found on the cell membrane of hepatocytes in cases of cirrhosis, alcoholic hepatitis and hepatitis C. CD25 (Interleukin-2 Receptor) Reduced expression of CD29 antigen together with the 2 integrin, CD11b, has been reported on peripheral blood lymphocytes from Graves' disease patients. Clone 4C9 1 mL, 0.1 mL lyophilized NCL-CD25-305 P (HIER) 7 mL Bond ready-to-use PA0305 P (HIER) See also Interleukin-2 Receptor (CD25) on page 125.
Primary Antibodies CD27
Clone 137B4 1 mL lyophilized NCL-CD27 F P (HIER) Human brain, cerebellum: immunohistochemical staining for CD29 antigen using NCL-CD29. Note membrane staining of endothelial cells. Paraffin section. CD27 antigen, a member of the nerve growth factor/tumor necrosis factor receptor superfamily, is a type I transmembrane protein consisting of a disulphide-linked 120 kD dimer. CD27 antigen is reported to be expressed on mature thymocytes and on the majority of human peripheral blood T lymphocytes, on both CD4 positive and CD8 positive subsets. CD27 antigen CD30 is also expressed on activated B lymphocytes and a proportion of resting NK cells. Among CD4 positive cells, CD27 antigen is preferentially expressed on unprimed CD4 positive/CD45RA positive/CD45RO negative T lymphocytes Clone JCM182 while primed CD4 positive/CD45RA negative/CD45RO positive T lymphocytes 1 mL, 0.1 mL liquid NCL-L-CD30-591 P (HIER) W Reference Range express low levels of CD27. During activation, the appearance of a 32 kD 7 mL Bond ready-to-use PA0790 P (HIER) New! soluble form results from proteolytic cleavage of the parent molecule. Soluble CD27 antigen has also been reported in cases of B cell chronic Clone 1G12 lymphocytic leukemia. 1 mL, 0.1mL lyophilized NCL-CD30 F P (HIER) 1 mL liquid NCL-L-CD30 F P (HIER) 7 mL ready-to-use RTU-CD30 F P (HIER) 7 mL Bond ready-to-use PA0153 P (HIER) Clone 15B3 1 mL, 0.1 mL lyophilized NCL-CD30-365 F P (HIER) Clone JCM182 was developed to be highly effective on formalin-fixed, paraffin-embedded tissue sections. The CD30 antigen is a single chain glycoprotein with a molecular weight of 120 kD. CD30 antigen is known to act as a receptor for a cytokine ligand, CD30L, and may also play a role in the regulation of cellular growth and transformation. CD30 antigen is reported to be expressed on the surface of multinucleated Reed Sternberg cells, mononuclear Hodgkin's cells and in the majority of anaplastic large cell lymphomas. The CD30 antigen is expressed in non-Hodgkin's lymphoma and virally transformed cells, eg EBV-transformed B cells. Most T cell lymphomas are reported not to express CD30 antigen, with the exception of some pleo-morphic T cell lymphomas.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. C-D111.Nt nes ebaesann fedteilcls aafnsection. Paraffin cells. endothelial of staining membrane using intense (PECAM-1) Note antigen NCL-CD31-1A10. CD31 for staining immunohistochemical glomangioma: Human during format. ready-to-use events Bond the interactive for 21 page in to Refer involved for essential is metastases. is and Angiogenesis growth it tumor healing. with wound that and domain thrombosis suggest cytoplasmic angiogenesis, of a properties antigen The has activation. cellular CD31 also after phosphorylation endothelial molecule for the cell sites stabilize CD31 potential to to The serve cell may that monolayer. and of contact cell adhesion, typical domain endothelial cell in subclass, to role extracellular cell a C2 endothelial plays mediates of an domain units This has molecules. homology adhesion Ig-like molecule six endothelial The the contains the on at expressed junction. and is cells molecule B intracellular CD31 granulocytes, The monocytes, kD. platelets, 140 of to surface with 130 glycoprotein of transmembrane weight chain molecular single a a is (PECAM-1) antigen CD31 PA0250 ready-to-use Bond mL 7 NCL-CD31-1A10 lyophilized mL 0.1 mL, 1 1A10 Clone (PECAM-1) CD31 Sternberg Reed of hofs section. paranuclear Paraffin of cells. staining characteristic and NCL-L-CD30-591. staining using membrane antigen Note CD30 for staining immunohistochemical lymphoma: Hodgkin's format. ready-to-use Bond the for 21 page to Refer in JCM182 reactivity. Clone background for increase recommended may datasheet that the than other solutions retrieval Using rdc pcfcInformation Specific Product (HIER) P (HIER) P ct yli ekma muoitceia tiigfrC3 nie sn NCL-L-CD33. using antigen CD33 for staining immunohistochemical leukemia: myeloid Acute format. ready-to-use Bond the for 22 page to CD33 Refer of distinguishing for leukemias. expression marker lymphoid important the The an from be myeloid to undifferentiated leukemias. demonstrated been acute lymphoblastic has on to antigen acute some restricted absent is and blasts, be CD33 to the leukemias of myeloid expression both reported premyelocytes, that on is reported monocytes, expressed it been has be although It to lineages, granulocytes. continues monocyte then and after It myeloid cells expression. precursor myelomonocytic antigen on appear CD34 to reported is antigen CD33 PA0555 ready-to-use Bond mL 7 NCL-L-CD33 liquid mL 0.1 mL, 1 PWS44 Clone CD33 tiigo epatcedteilclsadasneo tiigo toa el.Paraffin cells. stromal of staining of absence section. and intense cells Note endothelial NCL-END. neoplastic using of antigen staining CD34 for staining immunohistochemical tonsil: Human format. stronger ready-to-use Bond produces the for it 22 page as to Refer retrieval epitope staining. elastin induced background reduces heat and staining QBEnd/10 to Clone with preference recommended is in sections paraffin of digestion Enzyme CD34 a The Information cells. with Specific Product on progenitor glycoprotein endothelium. expressed vascular haemapoietic selectively on transmembrane expressed is myeloid also is protein protein chain and CD34 The lymphoid single kD. human 110 a of weight is molecular antigen CD34 PA0212 ready-to-use Bond mL 7 RTU-END ready-to-use mL 7 NCL-L-END liquid mL 1 NCL-END lyophilized mL 0.1 mL, 1 QBEnd/10 Clone Marker) Cell (Endothelial CD34 section. Paraffin cells. malignant of staining membrane and cytoplasmic intense Note W P F etr blotting Western Paraffin Frozen C I Ezm)C (Enzyme) P F lwcytometry Flow Immunofluorescence (Enzyme) P F HE)W (HIER) P (HIER) P (HIER) P Ezm)C (Enzyme) P F Reference Range O E Reference Range te applications Other lcrnmicroscopy Electron /77
Primary Antibodies CD35 CD38
Clone RLB25 Clone SPC32 1 mL, 0.1 mL lyophilized NCL-CD35 F P (Enzyme) 1 mL, 0.1 mL lyophilized NCL-CD38-290 F P (HIER) The CD35 antigen, also known as CR1 or C3b/C4b R, is a transmembrane 1 mL liquid NCL-L-CD38-290 F P (HIER) protein of 160 to 250 kD which binds complement components C3b and C4b. The CD38 molecule is a type II single transmembrane glycoprotein with a It mediates phagocytosis by neutrophils and monocytes of particles coated molecular weight of 46 kD. It is an ectoenzyme with the activities of ADP- with C3b or C4b. CD35 antigen has an inhibitory effect on complement ribosyl cyclase, cyclic ADP-ribose hydrolase, NAD glycohydrolase and is activation by both the classical and alternative pathways. CD35 antigen is involved in both the formation and hydrolysis of cADPR, a second reported to be found on erythrocytes, B cells, a subset of T cells, monocytes, messenger that regulates the mobilization of intracellular Ca2+ ions. macrophages cultured in vitro, neutrophils, eosinophils, glomerular Although the CD38 molecule was originally identified as a T lymphocyte podocytes and follicular dendritic cells. Decreased levels of CD35 antigen differentiation antigen, it is reported to be expressed in a wide range of cells has been reported on B cells in patients with HIV infection. and tissues. CD38 antigen can deliver potent growth and differentiation signals to lymphoid and myeloid cells. It is found on immature cells of the B and T cell lineages but not on most mature resting peripheral lymphocytes. It is also present on thymocytes, pre-B cells, germinal center B cells, mitogen- activated T cells, Ig-secreting plasma cells, monocytes, NK cells, erythroid and myeloid progenitors in the bone marrow and brain cells. CD38 antigen has also been reported in neurofibrillary tangles, the pathological indicator of Alzheimer's disease that occurs in the neuronal perikarya and proximal dendrites.
Normal human tonsil: immunohistochemical staining for CD35 antigen using NCL-CD35. Note intense membrane staining of follicular dendritic cells. Paraffin section. Primary Antibodies CD37
Clone CT1 Chronically inflamed human bronchus: immunohistochemical staining for CD38 antigen using 1 mL lyophilized NCL-CD37 F P (HIER) NCL-CD38-290. Note intense membrane staining of infiltrating activated T lymphocytes. Paraffin section. CD37 antigen is a member of the TM4 superfamily with a molecular weight of 40 to 52 kD. CD37 antigen was originally defined as an antigen of mature B lymphocytes where it is highly expressed. It is reported not to be expressed on pre-B cells or plasma cells and is expressed only at low level in T cells, neutrophils, monocytes and some myelomonocytic leukemia cells. CD39 NK cells, platelets and erythrocytes also do not express CD37 antigen. CD37 antigen on B cells associates non-covalently with MHC class II, CD19 and Clone 22A9 CD21 antigens and with other TM4 superfamily molecules CD53, CD81 and CD82. 1 mL lyophilized NCL-CD39 P (HIER) CD39 antigen is a transmembrane glycoprotein found on mature B lymphocytes, follicular dendritic cells, endothelial cells, activated T cells, NK cells and Langerhans cells. It is also known as E-type apyrase which hydrolyses extracellular ATP and ADP, a function important to homotypic adhesion and platelet aggression. CD39 antigen expressing cells may provide protection from the toxic effects of ATP leaked from damaged cells. CD39 antigen may enable tumor cells to reduce contact with T lymphocytes and escape immunological recognition. Increased levels of CD39 antigen expression are also reported to be associated with increased ectoATPase activity that occurs during the progression of melanomas.
Human lymph node, B cell lymphoma: immunohistochemical staining for CD39 antigen using NCL-CD39. Note intense membrane staining of tumor cells. Paraffin section.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. bec fC4batgno ltlt srpre ob osbeindicator possible a be to reported disease. is blood Bernard-Soulier platelets of on to The antigen leukemias. platelets CD42b megakaryoblastic and of in of platelets absence and on marrow binding expressed bone ristocetin- be in to the of megakaryocytes reported on is in co-factor antigen a CD42b involved The is walls. is vessel GPIb, and as aggregation known induced also glycoprotein, CD42b The NCL-CD42b lyophilized mL 1 MM2/174 Clone (GPIb) CD42b section. Frozen vessel. blood the within platelets NCL-CD41. aggregated using of (GPIIb/IIIa) staining antigen Note CD41 for staining immunohistochemical tonsil: Human throm- retraction Glanzmann's clot with binding, fibrinogen aggregation. patients in platelet involved from and is expressed platelets antigen be CD41 in The to and defective basthenia. leukemias reported or megakaryoblastic is absent in GPIIb/IIIa, and is maturation as megakaryocyte known in also early antigen, CD41 The NCL-CD41 lyophilized mL 1 M148 Clone (GPIIb/IIIa) CD41 some on expressed be to melanomas. malignant reported in and is carcinomas certain Outside antigen of cells. cells CD40 Sternberg epithelial system, Reed cells and immune B cells mature interdigitating the dendritic on lymphomas, and found follicular leukemias be cells, cell to reticulum B and reported most is proliferation cells), germinal antigen plasma of cell CD40 (except apoptosis The of B prevention cells. as the B in center such important of also functions transduction is the It cellular in differentiation. the involved for be of to signals factor/ appears function the it necrosis regulatory to precise but tumor unknown homology The the is significant antigen of CD30. CD40 a member antigen, shows a disease-associated be and to Hodgkin's superfamily known factor is molecular growth calculated It nerve a kD. with 27 glycoprotein chain of single weight a is antigen CD40 The NCL-CD40 lyophilized mL 0.1 mL, 1 11E9 Clone CD40 F (HIER) P F HE)W (HIER) P ee opg 2frteBn ed-oueformat. ready-to-use Bond the for 22 cases. page some to in Refer MT1 clone with staining enhance may pretreatment Enzyme CD43 Information Specific express Product to reported also chronic cell are B antigen. lymphomas of proportion SDS-PAGE centrocytic small A on and reported cells. are leukemias observed T antigen neoplastic kD CD43 and molecular normal the 140 include expressing exhibits to to Cells itself lines. 90 cell molecule of different T The between bands of lineage. cytoplasm with the myeloid heterogeneity in of weight and membrane cells the and on cells expressed is antigen CD43 The sections. paraffin on staining superior produces MT1 PA0938 Clone ready-to-use Bond mL 7 RTU-MT1 ready-to-use mL 7 NCL-L-MT1 liquid mL 1 NCL-MT1 lyophilized mL 1 MT1 Clone CD43 HCM sn C-D42 oeitnemmrn tiigo uo el.Prfi section. Paraffin cells. tumor of staining membrane antigen intense CD44 Note for NCL-CD44-2. staining using immunohistochemical (H-CAM) breast: of carcinoma cell squamous Human variants. antigen CD44 of been expression has the metastatic in it to changes Increased non-metastatic and with from associated carcinomas lines myocytes. be some cell may tumor on and of found transition fibroblasts that is be to reported antigen cells, reported to CD44 are glial of types substrate reported expression and cell cells, is cell positive erythrocytes epithelial antigen granulocytes, antigen of CD44 include monocytes, Other CD44 cells, regulation platelets. B migration. on the weakly cells, cell T in as on role expressed well a as glycoprotein receptor transmembrane suggesting interactions surface kD cell a hyaluronate, 95 is to antigen for The 80 glycosylation. an O-linked is extensive with (H-CAM) antigen CD44 The NCL-CD44-2 lyophilized mL 0.1 mL, 1 DF1485 Clone (H-CAM) CD44 section. Paraffin cells. tumor of staining NCL-MT1. membrane using intense antigen CD43 Note for staining immunohistochemical lymphoma: cell mantle Human W P F etr blotting Western Paraffin Frozen C I Ezm)W (Enzyme) P F lwcytometry Flow Immunofluorescence Ezm)W (Enzyme) P F (Enzyme) P F (HIER) P HE)C (HIER) P F O E te applications Other lcrnmicroscopy Electron /79
Primary Antibodies CD44 Variant Antibodies CD45
Clone VFF-327v3 Clone X16/99 1 mL lyophilized CD44 variant 3 NCL-CD44v3 F P (HIER) W 1 mL, 0.1 mL lyophilized NCL-LCA F P (HIER) C Reference Range 7 mL Bond ready-to-use PA0042 P (HIER) Clone VFF-8 1 mL lyophilized CD44 variant 5 NCL-CD44v5 F P (HIER) RP2/18, Clone RP2/22 1 mL lyophilized NCL-LCA-RP FP Clone VFF-7 1 mL liquid NCL-L-LCA-RP FP 1 mL lyophilized NCL-CD44v6 F P (HIER) 7 mL ready-to-use RTU-LCA-RP FP The CD44 molecule belongs to a family of cellular adhesion molecules found The CD45 antigen (leukocyte common antigen) is a family of five or more on a wide range of normal and malignant cells in epithelial, mesothelial and high molecular weight glycoproteins present on the surface of the majority hemopoiesis tissues. CD44 is a single gene with 20 exons, of which 10 are of the human leukocytes (including lymphocytes, monocytes and normally expressed to encode the basic CD44 (H-CAM) molecule. The eosinophils) but absent from erythrocytes and platelets. Various isoforms of additional 10 exons (v1 to v10) are only expressed by alternative splicing of CD45 are generated by alternative splicing of three exons. Expression of the nuclear RNA. The expression of specific cell adhesion molecule CD44 CD45 is necessary for signalling through the T cell receptor. NCL-LCA-RP is splice variants has been reported to be associated with metastasis in a cocktail of two antibodies, clone RP2/18 and RP2/22. certain human malignancies, such as breast cancer. A complex pattern of CD44 variant expression in different tumors compared to the CD44 Product Specific Information expression of the normal cell of origin has been reported (Fox SB et al., The heat induced epitope retrieval (HIER) technique may enhance staining in Cancer Research (53): 4539-4546, (1994)). High levels of expression were some cases with NCL-LCA-RP, NCL-L-LCA-RP and RTU-LCA-RP. observed with the variant exons by breast carcinomas that arise from breast ductal epithelium which do not normally express CD44. Conversely, normal Refer to page 22 for the Bond ready-to-use format. gastrointestinal epithelium were reported to express low levels of many of the CD44 variants and the derived colon cancers expressed low and variable levels of the variants. Respiratory epithelium which expressed variants at high levels in normal cells were reported to express the same variants at similar levels in lung carcinomas. Primary Antibodies
Human stomach, B cell lymphoma: immunohistochemical staining for CD45 antigen using NCL-L-LCA-RP. Note intense membrane staining of tumor cells. Paraffin section.
Human squamous cell carcinoma, floor of the mouth: immunohistochemical staining for CD44 variant 3 using NCL-CD44v3. Note intense membrane staining of tumor cells. Paraffin section. CD45RA Clone X148 1 mL lyophilized NCL-B1 FPC The CD45R subfamily comprises a restricted form of the leukocyte common antigen and is divided into four isoforms: CD45RA, CD45RB, CD45RC and CD45RO. The CD45RA molecule, a 220 kD isoform of CD45, is reported to be expressed on B cells, monocytes and a small proportion of T cells.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. ee opg 3frteBn ed-oueformat. ready-to-use Bond the for in 23 page staining to enhance Refer cell may T technique of (HIER) cases. The retrieval some majority expression. epitope cytoplasmic the induced exhibit heat on macrophages the tissue of and expression whereas surface antigen thymocytes show positive granulocytes CD4 of and of Monocytes percent malignancies. percent 37 lymphocytes, expressed 80 T be to blood lymphocytes, reported peripheral is of CD45, percent of 48 isoform on kD 180 a molecule, CD45RO The PA0146 ready-to-use Bond mL 7 RTU-UCHL1 ready-to-use mL 7 NCL-L-UCHL1 liquid mL 1 NCL-UCHL1 lyophilized mL 0.1 mL, 1 UCHL1 Clone CD45RO section. Paraffin cells. lymphoid neoplastic of using staining antigen membrane CD45RB intense for Note staining NCL-CD45RB. immunohistochemical lymphoma: cell B stomach, Human in staining enhance may cases. technique some (HIER) retrieval epitope induced heat The macrophages monocytes, Information cells, migratory Specific Product B a on CD45RB granulocytes. have found on inflammation. weakly cells be expressed of to is these sites and reported that to also thought recruited is with selectively individuals antigen is of are it circulation and and the advantage in infection increased EBV be cell to acute these reported of also percentage The is in joints. types accumulate arthritic which rheumatoid lymphocytes affected lymphocytes T within CD45RC T differentiated vivo CD45RB, highly positive of CD45RO CD45RA, subset on termed hematopoietica CD45RB defines of are of expression and cells Low types differently CD45RO. all and expressed cell on are lymphoid found isoforms various various be on The to erythrocytes. reported except is origin, molecule CD45 The NCL-CD45RB lyophilized mL 1 MEM55 Clone CD45RB HE)C (HIER) P F (HIER) P F (HIER) P F (HIER) P HE)C (HIER) P F ee opg 3frteBn ed-oueformat. ready-to-use Bond the for 23 page to Refer a and cells CD56 lymphocytes. NK The T cells, 5. activated Schwann of chromosome astrocytes, subset on neurons, located on gene expressed of single is splicing antigen a differential from by transcript generated RNA cell isoforms antigen the major CD56 closely-related four The of of cells. consists neural (NCAM) family development between embryogenesis, a interactions in role contact-mediated are a and play paraffin molecules to thought on adhesion glycoproteins surface cell staining neural superior The produce to developed sections. was CD564 Clone RTU-CD56-1B6 ready-to-use mL 1 NCL-L-CD56-1B6 liquid mL NCL-CD56-1B6 1 lyophilized mL 0.1 mL, 1 1B6 Clone PA0191 ready-to-use Bond mL 7 NCL-CD56-504 lyophilized mL 0.1 mL, 1 CD564 Clone (NCAM) CD56 123. page on (CD54) ICAM-1 also See NCL-CD54-307 lyophilized mL 0.1 mL, 1 23G12 Clone (ICAM-1) CD54 C-D654 oemmrn tiigo nei agincls aafnsection. Paraffin cells. ganglion enteric of using staining (NCAM) membrane antigen Note CD56 NCL-CD56-564. for staining immunohistochemical intestine: small Human W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence HE)W (HIER) P (HIER) P (HIER) P (HIER) P (HIER) P HE)W (HIER) P O E te applications Other lcrnmicroscopy Electron /81
Primary Antibodies CD57 CD62L (L-selectin)
Clone NK-1 Clone 9H6 1 mL, 0.1 mL lyophilized NCL-NK1 FP 1 mL lyophilized NCL-CD62L-489 P (HIER) 7 mL ready-to-use RTU-NK1 FP See also L-selectin (CD62L) on page 128. 7 mL Bond ready-to-use PA0443 P (HIER) The CD57 glycoprotein, also known as HNK-1, has a molecular weight of 110 kD. It is found on a subset of mononuclear cells with natural killer activity and on neuroectodermal cells expressing myelin-associated glycoprotein. CD62P (P-selectin) Many cells which co-express CD57 and CD8 proteins are a subset of suppressor/cytotoxic T cells. These cells play a role in the rejection of grafts in acute graft versus host disease. The CD57 molecule is not expressed on Clone C34 erythrocytes or platelets. 1 mL lyophilized NCL-CD62P-367 P (HIER) Product Specific Information See also P-selectin (CD62P) on page 155. Enzyme pretreatment may enhance staining in some cases. Refer to page 23 for the Bond ready-to-use format. CD63 (Melanoma Marker)
Clone NKI/C3 1 mL, 0.1 mL lyophilized NCL-CD63 FP CD63 antigen is a member of the TM4 superfamily with its structure consisting of four transmembrane regions, short cytoplasmic N and C- termini and two extracellular regions. CD63 antigen is widely distributed on the surface and interior of both hematopoietic and non-hematopoietic cells such as most sweat glands, islets of Langerhans, pituitary, pancreas,
Primary Antibodies peribronchial glands, Paneth cells and prostate glands. It is reported to be strongly expressed on monocytes, macrophages and activated platelets and Human lymph node: immunohistochemical staining for CD57 antigen using NCL-NK1. Note staining of CD57 positive T lymphocytes. Paraffin section. weakly expressed on lymphocytes and granulocytes. CD63 antigen associates non-covalently with CD9, CD81 and the integrins VLA-3, VLA-4 and VLA-6. It is reported that CD63 antigen may play a role as a tumor suppressor gene as its expression in human melanoma cells reduces tumor CD61 (GPIIIa) spread and metastasis.
Clone 2f2 1 mL, 0.1 mL lyophilized NCL-CD61-308 F P (HIER) CD66a (CEACAM1) 7 mL Bond ready-to-use PA0308 P (HIER) The CD61 antigen, also known as GPIIIa, is a glycoprotein of 105 kD found on Clone 29H2 platelets, monocytes, endothelial cells, smooth muscle cells, B cells, 1 mL lyophilized NCL-CD66a P (HIER) macrophages, mast cells and fibroblasts. CD61 antigen plays a role in CD66a antigen, also known as biliary glycoprotein (BGP), is a member of the platelet aggregation and also as a receptor for fibrinogen, fibronectin, von carcinoembryonic antigen (CEA) family and of the immunoglobulin super- Willebrand factor and vitronectin. Individuals with Glanzmann's throm- family. CD66a, CD66b, CD66c and CD66d are known to be expressed by basthenia are reported to express little or no CD61 antigen. CD61 antigen is hematopoietic cells. CD66a antigen is also expressed in epithelia, in certain also reported to be expressed in most cases of megakaryocytic leukemias. endothelia and in cells of the myeloid lineage. In liver, expression occurs in Refer to page 23 for the Bond ready-to-use format. hepatocytes along the bile canaliculi. Apical membranous expression of the antigen is reported to occur in enterocytes, superficial absorptive cells of the colon, epithelia of oesophageal and Brunner's glands, bile ducts and gall bladder, pancreatic ducts, proximal tubules of the kidney, prostate, endometrium and mammary ducts. Selective expression in endothelia is reported to occur in glomeruli and vasa recta of the kidney, small placental vessels, adrenal sinusoids, endometrium and prostate. Among the cells of the myeloid lineage, granulocytes and myelocytes express CD66a antigen. The expression of CD66a antigen has also been reported to be down- regulated in breast, endometrial, colorectal and prostate cancers. However, in one specific study of breast cancer, CD66a antigen was found not to be downregulated.
Human tonsil: immunohistochemical staining for CD61 antigen (GPIIIa) using NCL-CD61-308. Note staining of platelets within the blood vessels. Paraffin section.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. yolsi tiigo arpae.Prfi section. intense Paraffin Note macrophages. NCL-CD68. of using staining antigen cytoplasmic CD68 for staining immunohistochemical tonsil: Human is format. ready-to-use Bond protein the and for 24 cells page CD68 acid NK to Refer tubules. and associated renal by cells and liver T in surface as protein stiumlated attack such CD68 tissues in lysosomes. the non-haematopoietic demonstrated from the been from macrophages if has leakage membranes to expression unclear due the or significant of is functionally major protect monocytes, CD68 are It proteins the may membrane in identification hydrolases. of lysosomal and these function found but The components certain most the also lymphocytes. not the is large is molecule and are the for basophils CD68 antigen extent neutrophils, lesser CD68 However, a used to immunohistochemistry. to Markers and granules macrophages. frequently cytoplasmic reported of primarily with glycoprotein membranes associated intracellular kD be 110 a to is molecule sections. paraffin CD68 on The users end of choice main the is 514H12 Clone NCL-CD68-KP1 lyophilized mL 1 KP1 Clone PA0273 ready-to-use Bond mL 7 RTU-CD68 ready-to-use mL 7 NCL-L-CD68 liquid mL 1 NCL-CD68 lyophilized 0.1mL mL, 1 514H12 Clone CD68 67. page on (CD66e) Antigen Carcinoembryonic also See RTU-CEA-2 ready-to-use mL 7 NCL-L-CEA-2 liquid mL 1 NCL-CEA-2 lyophilized mL 1 12-140-10 Clone Antigen) (Carcinoembryonic CD66e section. Paraffin cells. epithelial absorptive of NCL-CD66a. staining using membrane antigen apical CD66a Note for staining immunohistochemical rectum: human Normal (HIER) P F (Enzyme) P F (Enzyme) P F (HIER) P F (Enzyme) P F (HIER) P F (HIER) P (HIER) P F CD71 section. Paraffin neutrophils. and cells NK lymphocytes, Note activated NCL-CD69. of using staining antigen membrane CD69 for staining immunohistochemical tonsil: Human and 6, T and activated 12 on chromosomes mainly on expressed complex is are gene CD69 a protein lymphocytes. B for as NK CD69 expressed genes the glycoprotein mouse respectively. membrane and within II human encoded type The a homodimer. is disulfide-linked molecule CD69 The NCL-CD69 lyophilized mL 0.1 mL, 1 CH11 Clone CD69 eepesdo l el fBcl ieg ihteecpino lsacells plasma of exception the macrophages. with tissue tyrosine lineage human directing cell on to protein B weakly fate reported of and receptor is cell the cells all protein antigen B on of CD72 determine expressed cell apoptosis. be vivo, to or B help differentiation in proliferation. regulate proliferation, BCR substrate, is either to from cell protein Signals a known signalling. B CD72 is is (BCR) addition, which and and In SHP-1 to expression unclear. phosphatase pathways bind is II signalling this to to class of of reported cells variety significance MHC B the a membrane of induce However, activate exposure integral the to can but II reported which unknown The is is type receptor. antibodies molecule cell the CD72 CD72 Kupffer the the of as of and such function CD23 molecules member receptors, surface cell asialoglycoprotein a related the other is includes which molecule glycoproteins CD72 The NCL-CD72 lyophilized mL 1 D748 Clone CD72 macrophages, cells, T and B activated neurons. an eg on cells iron, active is metabolically expressed and protein of cells CD71 be proliferating supply protein. to the iron-carrying major controlling reported and the binding by transferrin, the of through proliferation pathways, endocytosis metabolic cell many plays for in component molecule essential CD71 role molecular The subunits. a critical is kD and with 90 receptor a bonded transferrin glycoprotein the disulfide as two membrane known of is II composed It kD. type 180 a approximately of is weight molecule CD71 The NCL-CD71-309 lyophilized mL 0.1 mL, 1 10F11 Clone W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence (HIER) P (HIER) P (HIER) P O E te applications Other lcrnmicroscopy Electron /83
Primary Antibodies CD79b
Clone JS01 1 mL, 0.1 mL liquid NCL-L-CD79b P (HIER) CD79b, also known as B29 and Ig- is thought to function in the cellular activation and signalling that occurs when surface immunoglobulin (Ig) on B cells binds antigen or becomes cross-linked by anti-Ig antibody. This function occurs with the formation of a membrane signalling complex that is associated with Ig at the surface of B cells. CD79b, together with CD79a, forms the B cell antigen receptor (mlg) complex. CD79b expression is Human tonsil: immunohistochemical staining for CD72 antigen using NCL-CD72. reported to be found in 80 to 90 percent of mature B cell neoplasms, with the Note membrane staining of B lymphocytes. Paraffin section. exception of chronic lymphocytic leukemias.
CD79a
Clone 11E3 1 mL, 0.1 mL lyophilized NCL-CD79a-225 F P (HIER) 1 mL liquid NCL-L-CD79a-225 F P (HIER) C Reference Range 7 mL Bond ready-to-use PA0192 P (HIER)
Clone 11D10 Human tonsil: immunohistochemical staining for CD79b using NCL-L-CD79b. Note intense 1 mL, 0.1 mL lyophilized NCL-CD79a-192 F P (HIER) C membrane staining of B cells. Paraffin section. 1 mL liquid NCL-L-CD79a-192 F P (HIER) 7 mL ready-to-use RTU-CD79a-192 F P (HIER) Primary Antibodies Clone 11E3 was developed to produce superior staining on paraffin sections. CD81 The CD79 complex is a disulfide-linked heterodimer which is non-covalently associated with membrane-bound immunoglobulins on B cells. This complex of polypeptides and immunoglobulin constitute the B cell antigen receptor. Clone 1D6 The two components of this complex are designated CD79a and CD79b. The 1 mL lyophilized NCL-CD81 P (HIER) CD79a antigen is reported to first appear at the pre-B cell stage, early in CD81 antigen, also known as TAPA-1, is a member of the TM4 superfamily maturation, and persist until the plasma cell stage where it is found as an and is predicted to have four transmembrane regions, short cytoplasmic intracellular component. The CD79a antigen is reported to be expressed in N and C-termini and two extracellular regions. CD81 protein has a molecular the majority of acute leukemias of precursor B cell type, B cell lines, B cell weight of 26 kD and is expressed by most cell types. Of the hematopoietic lymphomas and in some myelomas. It is not present in myeloid or T cell lines. cells, CD81 protein is reported to be expressed by B and T cells, Refer to page 24 for the Bond ready-to-use format. macrophages, dendritic cells, NK cells and eosinophils but not by neutrophils, platelets or erythrocytes. The CD81 protein associates non- covalently with a number of other molecules eg CD19, CD21, MHC class I and II, CD20, CD37, CD53 and CD82 in B cells and CD4, CD8 and CD82 in T cells. It also associates with the integrins CD29/CD49c (VLA-3), CD29/ CD49d (VLA-4) and CD29/CD49f (VLA-6) in several other cell types. No extracellular ligand has been identified for CD81 protein and its function remains unclear, although mouse CD81 protein plays a role in early T cell development. The human CD81 molecule has been reported to be involved in cell adhesion, motility, metastasis as well as cell activation and signal transduction.
Human large cell lymphoma: immunohistochemical staining for CD79a antigen using NCL-CD79a-225. Note membrane staining of tumor cells. Paraffin section.
Human tonsil: immunohistochemical staining for CD81 antigen using NCL-CD81. Note membrane staining of most cell types including B and T lymphocytes and vascular endothelial cells. Paraffin section.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. sn C-D3 oemmrn tiigadcaatrsi aaula oso Reed of section. hofs Paraffin paranuclear cells. characteristic Sternberg and staining antigen membrane CD83 Note for NCL-CD83. staining using immunohistochemical type: cellularity mixed disease, Hodgkin's peripheral activated and cells antigen. B CD83 of express center also lymphocytes characteristic during germinal lymphocytes are a specific Some cells tumor is activated dendritic carcinogenesis. of activated recruitment This and the for organs. mature essential where lymphoid dendritic/T reactions resembles secondary immune which be ongoing cells, of T may to clusters amongst cells most areas positive reported cell in peripheral CD83 expressed mature in is be carcinoma, found to breast antigen be and found In be CD83 cells. can cells Sternberg and cells. Reed disease dendritic Hodgkin's dendritic in immunostimulatory positive expressed blood contain In to include antigen organs. reported peripheral lymphoid is These CD83 of decidua zones skin, cells. pregnancy, cell human T dendritic early the the within activated cells in reticulum and interdigitating to cells reported mature is on superfamily, Langerhans immunoglobulin expressed the of be member a antigen, CD83 NCL-CD83 lyophilized mL 0.1 mL, 1 1H4b Clone CD83 section. Paraffin cells. tumor of using staining antigen membrane CD82 intense for Note staining NCL-CD82. immunohistochemical carcinoma: cell squamous skin, Human showed cancer prostate untreated from glandular expression. of variable tissue membrane more cellular However, the suppress in cells. of to uniform expression be epithelial reported the to found hyperplasia, is was prostatic antigen antigen benign CD82 In cells CD82 be cells. T tumor of cells, to in B expression metastasis in reported signals The cells, transduce is erythrocytes. monocytes. can T it expression and in and vitro, antigen in not B and, CD82 upregulated but including strongly activation, platelets TM4 types, the lymphocyte and cell of Upon granulocytes member most a monocytes, in is cells, expressed antigen, NK C33 is or It KAI1 as superfamily. known also antigen, CD82 NCL-CD82 lyophilized mL 1 5B5 Clone CD82 (HIER) P (HIER) P ee opg 4frteBn ed-oueformat. ready-to-use Bond the for 24 page Ewing’s CD99 to including Refer tumors, cells. of lymphoma. range epithelial (except cell wide T a and squamous types in sarcoma reported basal/parabasal cell been ovary, has the and hematopoietic expression of all cells cells granulose virtually endothelial lymphocytes, blood cells, cells, peripheral islets Sertoli granulocytes), pancreatic thymocytes, and on cortical expressed Leydig be including cells, to tissues immature reported of T human is apoptosis most CD99 of molecules, diapedesis. MHS leukocyte in aggregation and and implicated receptor thymocytes homotypic been cell T has including of CD99 upregulation processes understood, fully cellular CD99 not various is (p30/32). the kDa function 32 of and its Y two splicing 30 Although of and encode alternative bands as X to by identified are human shown produced and transcript been the are gene has of which gene region proteins MIC2 distinct pseudoautosomal gene, the the MIC2 Recently, the in by chromosomes. encoded located glycoprotein, is transmembrane kDa which 32 a is CD99 PA0509 ready-to-use Bond mL 7 12E7 Clone NCL-L-CD99-187 liquid mL 0.1 mL, 1 PCB1 Clone CD99 110. page on (CD95) Fas also See NCL-FAS-310 lyophilized mL 1 GM30 Clone (Fas) CD95 e loEdgi C15 npg 103. page on (CD105) Endoglin also See NCL-CD105 lyophilized mL 0.1 mL, 1 4G11 Clone (Endoglin) CD105 using section. CD99 Paraffin for NCL-L-CD99-187. staining immunohistochemical tumors: neuroectodermal primitive Human W P F etr blotting Western Paraffin Frozen New! C I lwcytometry Flow Immunofluorescence (HIER) P F P (HIER) P (HIER) P O E te applications Other lcrnmicroscopy Electron /85
Primary Antibodies CD117 (c-kit Oncoprotein)
Clone T595 1 mL lyophilized NCL-CD117 P (HIER) 1 mL liquid NCL-L-CD117 P (HIER) Reference Range 7 mL ready-to-use RTU-CD117 P (HIER) Clone 57A5D8 1 mL lyophilized NCL-cKIT F
See also c-kit Oncoprotein (CD117) on page 91. Human tonsil: immunohistochemical staining for CD134 antigen using NCL-CD134. Note intense membrane staining of activated T lymphocytes. Paraffin section.
CD123 CD137 Clone BR4MS New! 1 mL, 0.1 mL liquid NCL-L-CD123 P (HIER) Clone S16 1 mL lyophilized NCL-CD137 P (HIER) The CD123 antigen is also known as the alpha subunit of the human interleukin-3 receptor. It is a type I transmembrane glycoprotein and is a CD137 antigen, a member of the tumor necrosis factor receptor family, and member of the cytokine receptor superfamily. CD123 forms a heterodimer its ligand are reported to be expressed on activated T lymphocytes and on with CD131 (the beta subunit of the interleukin-3 receptor) to form the antigen-presenting cells, respectively. This receptor/ligand system interleukin-3 receptor, where the cytokine specificity is provided by the regulates the activation, proliferation and survival of T and B lymphocytes alpha subunit and the signal transduction function is provided by the beta and monocytes through bidirectional signal transduction. Human CD137 subunit. The interleukin-3 receptor is reported to be expressed on antigen is reported to be expressed on activated B cells, Reed Sternberg monocytes, neutrophils, basophils, eosinophils, megakaryocytes, cells and peripheral blood monocytes but is absent from resting T cells. In proliferation and differentiation of these cells. Outside the hematopoietic nonlymphoid cells, expression has been reported in blood vessel walls, on
Primary Antibodies system CD123 is reported to be expressed in Leydig cells of the testis, some the endothelial layer and on vascular smooth muscle cells. Soluble forms of endothelial cells, and cells of the placenta and brain. CD137 are reported at increased levels in sera of individuals with rheumatoid arthritis. The expression of soluble CD137 lags behind that of membrane bound CD137 by approximately 24 hours and it has been proposed that as activation of lymphocytes through membrane-bound CD137 delivers a potent stimulatory signal then soluble CD137 may provide a negative control mechanism for immune responses.
Human high venule endothelium and plasmacytoid dendritic cells: immunohistochemical staining for CD123 using NCL-L-CD123. Paraffin section.
Human tonsil: immunohistochemical staining for CD137 antigen using NCL-CD137. Note intense CD134 (OX40) membrane staining of activated lymphoid cells. Paraffin section.
Clone 102H6 1 mL lyophilized NCL-CD134 F P (HIER) The CD134 molecule is a member of the tumor necrosis factor receptor CD141 (Thrombomodulin) superfamily. It was originally named MRC OX40 after the first antibody which led to its discovery. CD134 protein binds to OX40 ligand and is expressed Clone 15C8 specifically on activated T lymphocytes with maximum expression deter- 1 mL, 0.1 mL lyophilized NCL-CD141 F P (HIER) mined at twenty four hours post stimulus. In rats, CD134 protein is only expressed on activated CD4 positive T lymphocytes and in humans it is See also Thrombomodulin (CD141) on page 162. described as being found predominantly on CD4 positive cells. In mice, CD134 protein is expressed on both activated CD4 positive and CD4 positive/ CD8 positive cells. The OX40 ligand binds CD134 protein on T cells and co- stimulates proliferation. Crosslinking of CD134 with OX40 ligand on activated B cells stimulates proliferation and antibody production suggesting a role in B cell differentiation into plasma cells. Functionally, the CD134 molecule is involved in T cell co-stimulation and T cell dependent antibody production.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. u sntdtcal ntebuhbre.Hg xrsino D5 a also has carcinomas. CD151 cell of lung expression small High in border. reported brush cells, enterocytes been the villous on and muscle detectable crypt not by is expressed cardiac but be small of to the and cells reported In is cells. basal CD151 smooth endothelial intestine, in and megakaryocytes expressed skeletal, platelets, be cells, cells, schwann to reported epithelial been cancer. epidermis, has and signalling as molecule differentiation such processes CD151 intracellular proliferation, cellular The activation, of and motility, range wide adhesion, between domains a cell in linkers involved and chain as are loop They act alpha cytoplasmic molecules. They small integrin domains. a carboxy-terminal loop, extracellular and extracellular amino large one short by family the characterized and of are small member Tetraspanins one a proteins. is transmembrane PETA-3/SFA, tetraspanin as of known also molecule, CD151 The NCL-CD151 lyophilized mL 1 RLM30 Clone (PETA-3) CD151 cells. endothelial and section. melanocytes Paraffin metastatic of staining using membrane antigen Note CD146 NCL-CD146. for staining immunohistochemical melanoma: malignant Human carcinoma In breast cases. in some cells. through suppressor in lost tumor endothelial frequently a possibly expression as and with act melanoma, may cells is protein human melanoma CD146 molecule contrast, in between receptor/CD3 protein CD146 progression interaction cell CD146 cells. enhanced tumor the T T activated promote of and that homing can and superantigen suggest extravasation the antigen, in reports involved recall Furthermore PHA, all on stimulation. via induced be can cells it CD146 ligand. molecule T uncharacterized adhesion yet glycoprotein, cell as group a an blood with as human interacts functions the molecule to CD146 related Although considerable closely lutheran. goldfish also gicerin, shares is chicken and molecule, It neurolin adhesion neural V-V- structure. chicken characteristic with the domain homology with kD immunoglobulin-like 113 in of CD146 C2-C2-C2 on glycoprotein formation Structurally, expressed membrane cortex. metastasis integral be cerebellar an to and and is reported muscle been progression smooth has cells, tumor it endothelial recently, of More marker melanoma. cell metastasis-associated human a melanoma was melanoma molecule the as CD146 the and the defined Originally, Mel-CAM. S-Endo-1 as or antigen, MCAM molecule, known A32 adhesion MUC18, also molecule, is surface protein CD146 NCL-CD146 lyophilized mL 0.1 mL, 1 N1238 Clone (MCAM) CD146 (HIER) P HE)W (HIER) P ehns fslbeC13atgnb ua ooye a lyan release play specific may no monocytes a human that has processes. by inflammatory proposed and modulating antigen in mediator, been role CD163 important glucocorticoids has soluble inflammatory of It by an mechanism phorbol expression. by upregulated on lipopolysaccharide, and influence is A while express cyclosporin to protein immunosuppressant esters, multi- not the addition, The reported by are downregulated In protein. lesions cells. inflammatory follicles, CD163 Langerhans lymphoid within of and its cells be centers germinal nucleated cells in to and reticulum zone except reported restricted macrophages mantle interdigitating is tissue the is most in M130 It and found protein as monocytes those lineage. circulating known CD163 all monocytic/macrophage also on SM4. protein present the or membrane to I Ki-M8 expression type Ber-Mac3, a is antigen, molecule CD163 The NCL-CD163 lyophilized mL 0.1 mL, 1 10D6 Clone CD163 section. Paraffin endothelium. and intense membrane Note basement NCL-CD151. of using staining CD151 for staining immunohistochemical placenta: Human nes ebaeadctpamcsann fvlosmcohgs aafnsection. Paraffin macrophages. villous of staining Note cytoplasmic NCL-CD163. and using membrane antigen intense CD163 for staining immunohistochemical placenta: Human W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence (HIER) P O E te applications Other lcrnmicroscopy Electron /87
Primary Antibodies CD166 (ALCAM) CD205 (DEC-205)
Clone MOG/07 Clone 11A10 1 mL, 0.1 mL lyophilized NCL-CD166 P (HIER) 1 mL liquid NCL-L-DEC205 P (HIER) The human CD166 molecule, also known as activated leukocyte cell CD205 is a 205 kD integral membrane glycoprotein homologous to the adhesion molecule (ALCAM), is a glycoprotein of 100 kD that functions as a macrophage mannose receptor and related receptors. It is a novel ligand for the CD6 molecule. It is the human homolog of the chicken neural multilectin, endocytic receptor that can be used by dendritic cells and adhesion molecule, BEN/SC-1/DM-GRASP, the rat molecule, KG-CAM, and thymic epithelial cells to direct captured antigens from extracellular spaces the fish protein, neurolin. The CD166 molecule is reported to be expressed by to a specialized antigen processing compartment. a subset of activated leukocytes. CD166/CD6 interactions may play a role in the binding of T and B cells to activated leukocytes as well as in interactions between cells of the nervous system involving neurite extension of the neurons. The CD166 molecule is also expressed in a number of other cell types including activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells and also in sweat and sebaceous glands. CD166 protein expression is reported to be upregulated in a cell line deriving from a metastasizing melanoma. It is also reported that CD166 protein may play a role in T cell development in the thymus.
Human thymus: immunohistochemical staining for DEC-205 using NCL-L-DEC205. CD168 (RHAMM) Note cytoplasmic and membrane staining of epithelial cells. Paraffin section. Clone 2D6 1 mL lyophilized NCL-CD168 F P (HIER) The CD168 molecule, also known as RHAMM/IHABP (receptor for CD243 (P-glycoprotein) hyaluronic acid mediated motility/intracellular hyaluronic acid binding
Primary Antibodies protein), is a ubiquitously expressed filamentous, cytoskeletal accessory Clone 5B12 protein. It is not, as originally reported, a cell surface receptor. However, in some cancers, it is reported that the expression of cell surface variants of 1 mL lyophilized NCL-PGLYm F P (HIER) CD168 is closely correlated with tumor progression. The CD168 molecule See also P-glycoprotein (CD243) on page 150. plays a role in cell signalling, migration and adhesion via interactions with hyaluronan, microtubules, actin, calmodulin and components of the extracellular regulated kinase (erk) signalling pathway. CD168 appears to have an important role in human sperm motility. In the brain, the CD168 molecule is reported to be expressed in the majority of neurons and in many CD246 (Anaplastic Lymphoma Kinase) oligodendrocytes where it has an effect on astrocyte motility, neurite migration and axonal growth. CD168 antigen is necessary for migration of (ALK) (p80) smooth muscle cells after wound injury and it has been associated with adult wound fibroplasias. Reports indicate that CD168 antigen is detected at Clone 5A4 varying levels in normal breast epithelium but in breast cancers, strong expression has been observed particularly in well-differentiated grade 1 1 mL, 0.1 mL lyophilized NCL-ALK P (HIER) ductal carcinomas, whereas high grade cancers displayed significantly 7 mL Bond ready-to-use PA0306 P (HIER) lower expression. CD168 is also reported to be expressed at low frequency in non-cancerous gastric mucosa and in 74 percent of gastric cancers See also ALK (Anaplastic Lymphoma Kinase) (CD246) (p80) on page 54. where it is associated with malignant progression.
cdk-activating kinase (CAK)
Clone MO-1.1 1 mL lyophilized NCL-CAK FWO Cyclin dependent kinase (cdk) activation is dependent on cyclin binding and phosphorylation of a conserved threonine residue mediated by cdk- activating kinase (CAK). Human CAK has been identified as the p40MO15 (cdk7)/cyclin H/MAT1 complex which is localized to the nucleus in vertebrates. DNA repair mechanisms and regulation of gene activity both Normal human testis: immunohistochemical staining for CD168 antigen using NCL-CD168. involve CAK. NCL-CAK detects the catalytic subunit p40MO15 (cdk7). Note membrane staining of spermatocytes in the seminiferous cells. Paraffin section. Product Specific Information NCL-CAK may also be used in immunoprecipitation techniques.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. rti perdt orlt ihpoieaino omladmalignant and CAS normal cytoplasmic of non- of proliferation grade expression with cells. high Low correlate cells lymphoid that all to lymphomas. of proposed appeared percent cell was 60 protein to It B 10 null positive. only and large where were and T reported diffuse were of leukemias express lymphoma lymphomas and Hodgkin's cells anaplastic some malignant cell phenotype the of large of cell include percent development 80 These disease to protein. the up CAS Hodgkin's where in and reported the been lymphoma that role have proposed non-Hodgkin's been a Aggressive has proliferation it play lymphomas. but cell understood may with fully associated yet protein is not human CAS is the apoptosis which and by is molecular is The function CAS CSE-1. or gene, spindle. gene, mechanism chromosome-segregation CAS mitotic yeast the the the of of and homolog product microtubules the with protein, associated susceptibility apoptosis Cellular NCL-CAS lyophilized mL 1 30F12 Clone Protein Susceptibility Apoptosis Cellular 82. page on (CEACAM1) CD66a also See NCL-CD66a lyophilized mL 1 29H2 Clone (CD66a) CEACAM1 section. Paraffin cells. epithelial of staining cytoplasmic using and protein nuclear homeobox Note CDX2 NCL-CDX2. for staining immunohistochemical intestine: small Human format. ready-to-use Bond the for 25 page to Refer the the cell with of cancer decreases gastric carcinogenesis differentiated progressively poorly lines. with to mRNA differentiated associated CDX2 well from contribute of be transition been expression to also has the as reported may It of stomach cancers. been CDX2 portions colorectal has that distal sporadic some intense the CDX2 reported of CDX1, but of progression to all the loss contrast in towards In The occur crypts. intestine. to the in reported developing and is villus expression the CDX2 expressed of spatial primarily surface is different CDX2 the and epithelium. on function intestinal of its the Phosphorylation in modulate patterns can genes. expression only domain homeobox the activation of is for CDX2 pattern intestine unusual the This the product. is as gene expression either interest of the cells. restricted particular levels of in detectable epithelial contains is involved intestinal that CDX1, organ be of as may well differentiation as and and CDX2, development proliferation intestinal of in regulation that early factor expressed transcription intestine-specific is homeobox, caudal-type a is CDX2 PA0535 ready-to-use Bond mL 7 NCL-CDX2 lyophilized mL 0.1 mL, 1 AMT28 Clone CDX2 HE)W (HIER) P F (HIER) P (HIER) P (HIER) P Reference Range Lrayt-s E- etra oan RTU-CBE-356 domain) (external HER-2 ready-to-use mL NCL-L-CBE-356 7 domain) (external HER-2 liquid mL 1 domain) (external NCL-CBE-356 HER-2 lyophilized mL 0.1 mL, 1 10A7 Clone TA9145 System IHC Bond HER2 Oracle Tests RTU-CB11 60 domain) (internal HER-2 ready-to-use mL 7 NCL-L-CB11 domain) (internal HER-2 liquid mL 1 domain) (internal NCL-CB11 HER-2 lyophilized mL 0.1 mL, 1 CB11 Clone NCL-c-erbB-2-316 domain) (internal HER-2 lyophilized mL 1 5A2 Clone Antibodies (HER-2) Oncoprotein c-erbB-2 cells. tumor proliferating of staining section. nuclear Paraffin and cytoplasmic Note susceptibility NCL-CAS. apoptosis using cellular protein for staining immunohistochemical carcinoma: breast Human o oeifraino h rceHR odICSse e ae11. page see System IHC Bond HER2 Oracle the on information more 119. For page on Antibodies Oncoprotein) (c-erbB-2 HER-2 also See NCL-PC11 domain) (internal HER-2 lyophilized mL 0.2 Polyclonal NCL-CBE1 lyophilized mL 1 CBE1 Clone W P F etr blotting Western Paraffin Frozen FPC (HIER) P F FPC PW E- etra domain) (external HER-2 FP Reference Range C I lwcytometry Flow Immunofluorescence O E te applications Other lcrnmicroscopy Electron PW FP FP /89 P
Primary Antibodies c-erbB-3 Oncoprotein
Clone RTJ1 1 mL lyophilized NCL-c-erbB-3 F P (HIER) O The c-erbB-3 oncoprotein is a member of the type 1 growth factor receptor family which also includes c-erbB-2 and epidermal growth factor receptor (EGFR). These receptors share a common overall structure consisting of an extracellular domain, a transmembrane region and a cytoplasmic domain. The expression of c-erbB-3 oncoprotein has been reported in chronic pancreatitis, exocrine pancreatic cancer and in tumors of the gastro- intestinal tract. Human tonsil: immunohistochemical staining for checkpoint kinase 1 using NCL-Chk1. Product Specific Information Note nuclear staining of proliferating cells. Paraffin section. NCL-c-erbB-3 recognizes an epitope in the cytoplasmic domain of the human c-erbB-3 oncoprotein and does not cross-react with c-erbB-2 or EGFR. NCL- c-erbB-3 may also be used in immunoprecipitation techniques. Cholecystokinin (CCK-8) Polyclonal 0.25 mL lyophilized NCL-CCK-8p P (Enzyme) See also CCK-8 (Cholecystokinin) on page 69.
Choline Acetyltransferase
Human rectal adenocarcinoma: immunohistochemical staining for c-erbB-3 oncoprotein using Clone 38B12
Primary Antibodies NCL-c-erbB-3. Note cytoplasmic staining characteristic in these tumors. In other tumor types, 1 mL lyophilized NCL-ChAT P (HIER) membrane staining is observed. Paraffin section. Choline acetyltransferase (ChAT) is a 68 kD enzyme which catalyzes the synthesis of acetylcholine (ACh) from choline and acetyl coenzyme A. The human ChAT gene encodes two proteins, the 68 kD ChAT enzyme and a 27 kD protein immunologically related and coexpressed with ChAT in c-fos Oncoprotein cholinergic neurons of the central nervous system. The smaller protein may play a role in the regulation of ACh synthesis. ChAT is expressed in Clone CF2 cholinergic neurons, the majority of the neurons in the nucleus basalis of Meynert, large neurons in the striatum (putamen and caudate nuclei), the 1 mL lyophilized NCL-FOS F majority of neurons in the pedunculopontine, hypoglossal, dorsal nucleus of The c-fos proto-oncogene encodes a nuclear phosphoprotein and is a vagus and subgroups of neurons in Roller's and the medial olivary regulator of transcription, forming a complex with the c-jun proto-oncogene accessory nuclei. Prominent staining is observed in ribonucleoprotein, product. Expression of the c-fos gene is reported to be low in most adult distributed at the periphery of large neurons of the nucleus basalis of tissues, however, high levels of expression have been detected in normal Meynert, the motor neurons in the hypoglossal and ambiguus nuclei. skin. A negative correlation between p53 protein expression and c-fos Product Specific Information oncoprotein expression has been reported in human basal cell carcinomas of skin. NCL-ChAT does not label axons in the insular cortex of the internal capsule non-cholinergic structures, endothelial cells or microglia.
Checkpoint Kinase 1
Clone DCS-310.1 1 mL lyophilized NCL-Chk1 P (HIER) Checkpoint kinase 1 (Chk1) is an evolutionary conserved protein, which in its phosphorylated form regulates cell cycle progression in response to agents that block DNA replication. Eukaryotic cells which are exposed to ionizing radiation (IR) or other genotoxic stresses activate checkpoints to delay the progression of the cell cycle. Defects in the IR-induced S phase checkpoint Human brain, basal ganglia: immunohistochemical staining for choline acetyltransferase using causes radioresistant DNA synthesis, a phenomenon reported in cancer- NCL-ChAT. Note cytoplasmic staining of neurons of the nucleus basalis of Meynert. Paraffin prone individuals suffering from ataxia-telangiectasia. Both of the cell cycle section. kinases, Chk1 and Chk2, act upstream of p53 in DNA damage responses. It has been reported that Chk1 mutations have been implicated in the cause of some cancers of families with Li-Fraumeni syndrome.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. C-JN oencersann ftmrcls aafnsection. Paraffin cells. tumor of staining using nuclear oncoprotein Note c-jun NCL-cJUN. for staining immunohistochemical carcinoma: breast Human normal affect may transcriptional consequence a the function. as and affect and growth cell genes may target an oncogene transduction expression specific signal of plays c-fos c-jun and initiation expression in oncoprotein the gene Alterations c-jun of with regulation processes. heterodimer. the interacts in transacting and role important a AP1 component major avian forming factor a of is product transcription which c- gene The p39, the family. transforming protein, gene nuclear of early-response the a the encodes of of member oncogene homolog a jun is cellular and 17 normal virus sarcoma the is c-jun NCL-cJUN lyophilized mL 1 DK4 Clone Oncoprotein c-jun section. Paraffin cells. islet neoplastic of staining cytoplasmic NCL-CHROM-430. intense using Note A chromogranin for staining immunohistochemical insulinoma: Human format. ready-to-use Bond the for carcinomas, 25 page to thyroid Refer medullary carcinoids. adenomas, and phaeochromocytomas, pituitary tumors cell as tumors, Merkel such expression tumors cell A Chromogranin neuroendocrine islet thyroid. islet in the gland, reported of pituitary cells widelybeen C anterior has be and medulla, endocrine pancreas to human the adrenal reported of of gland, cells is granules parathyroid which secretory in eg protein and cells acidic tissues kD neural 68 in expressed a is A Chromogranin PA0430 ready-to-use Bond mL 7 NCL-CHROM-430 lyophilized mL 0.1 mL, 1 5H7 Clone A Chromogranin (Enzyme) P F (HIER) P (HIER) P atclritrs ntesuyo atonetnlsrmltmr (GIST), tumors stromal gastrointestinal melanomas. hematopoiesis, in of and during of carcinomas study cell is role lung the antigen small in CD117 a of interest plays expression particular The c-kit melanogenesis. family. and gametogenesis platelet-derived receptor the to factor closely-related is growth which tyrosine (CD117), with c-kit receptor activity, transmembrane kinase a encodes proto-oncogene c-kit The NCL-cKIT lyophilized mL 0.1 mL, 1 57A5D8 Clone RTU-CD117 ready-to-use mL 7 NCL-L-CD117 liquid mL NCL-CD117 1 lyophilized mL 0.1 mL, 1 T595 Clone (CD117) Oncoprotein c-kit C-LSEI.Nt nes ebaesann ffliua el.Prfi section. Paraffin cells. follicular of staining membrane using intense J) Note (apolipoprotein NCL-CLUSTERIN. clusterin for staining immunohistochemical tonsil: Human anaplastic in expressed This be lymphoma. to death index. cell reported cellular large been also to apoptotic has prerequisite Clusterin a with apoptosis. not size by was breast inversely tumor expression clusterin In with correlated that correlated deposits. suggested upregulated, be cerebrovascular to when reported and and, and is plaques expression systemic to clusterin amyloid reported cancers, in in is clusterin deposits present disease, Alzheimer's cascade,be fibrillar amyloid In and an disorders. with adhesion complement amyloid is localized co-localizing cell it the protein conditions, regulation, pathological associated immune of In death, transformation. regulation morphological cell multi- recycling, transport, sperm ubiquitous, including membrane processes a lipid biological numerous is in It maturation, alpha implicated subunits, is T64. disulfide-linked It two and beta. of and comprising TRPM2 kD 80 40, of protein SP functional gp80, SGP-2, inhibitor, lysis III, complement J, glycoprotein apolipoprotein as known also is Clusterin NCL-CLUSTERIN lyophilized mL 1 7D1 Clone J) (Apolipoprotein Clusterin section. Paraffin cells. mast and melanocytes of proportion a NCL-CD117. of using staining (CD117) membrane oncoprotein Note c-kit for staining immunohistochemical skin: Human W P F etr blotting Western Paraffin Frozen C I (HIER) P lwcytometry Flow Immunofluorescence (HIER) P (HIER) P F Reference Range (HIER) P O E te applications Other lcrnmicroscopy Electron /91
Primary Antibodies c-MET (Hepatocyte Growth Factor Collagen Type IV
Receptor) Clone PHM-12 1 mL lyophilized NCL-COLL-IV F P (HIER+Enzyme) Clone 8F11 1 mL, 0.1 mL lyophilized NCL-cMET F P (HIER) In kidney, collagen IV is expressed in glomerular and tubular basement membranes and also mesangial cells and the matrix within glomeruli, the The c-MET gene encodes a transmembrane tyrosine kinase identified as the basal lamina of capillaries as well as basement membrane structures in receptor for a polypeptide known as hepatocyte growth factor (HGF). HGF many organs. has been shown to exert a pleiotropic activity on several cell types mainly of Product Specific Information epithelial origin. It is a powerful mitogen for hepatocytes and also stimulates the growth of other cell types including kidney tubular cells, keratinocytes The heat induced epitope retrieval (HIER) technique followed immediately by and endothelial cells. Other cell types known to express c-MET include 30 seconds of enzyme digestion produces optimal staining with this antibody. hepatocytes, microglial cells in white matter and astrocytes. NCL-COLL-IV recognizes collagen type IV, which is a major constituent of basement membranes.
c-myc Oncoprotein
Clone 9E11 1 mL, 0.1 mL lyophilized NCL-cMYC FP The c-myc oncogene is the human cellular homolog of the avian v-myc gene found in several leukemogenic retroviruses. c-myc is a nuclear phospho- protein, which has DNA-binding activity and is implicated in the control of normal proliferation and differentiation. Expression of c-myc in untransformed cells is growth factor dependent and essential for Normal human kidney: immunohistochemical staining for collagen type IV using NCL-COLL-IV. progression through the cell cycle. c-myc is expressed during proliferation Note staining of tubular basement membranes, mesangial cells and the glomerular matrix. in a wide variety of adult tissues and at all stages of embryonic Paraffin section.
Primary Antibodies development. Product Specific Information Enzyme pretreatment may enhance staining in some cases. Collagen Type VII
Clone LH7.2 Collagen Type II 1 mL lyophilized NCL-COLL-VII F Collagen type VII is a basement membrane component which is the major Polyclonal protein in the anchoring fibrils projecting from the lamina densa into the 1 mL lyophilized NCL-COLL-IIp P (Enzyme) subjacent connective tissue. Collagen type VII has been reported to be detected in the basal lamina of stratified epithelia such as epidermis, oral, Collagen type II is the structural protein predominantly found throughout the oesophageal and cervical epithelium and urothelium of the bladder. Those cartilage matrix and is also found in very small amounts in the eye. The epithelia which are composed of different cell types eg sweat gland fibrils formed by this protein are usually thinner and more delicate than epithelium or breast epithelium which are made up of myoepithelial cells collagen type I fibrils. next to glandular cells, possess a type VII collagen-containing basement Product Specific Information membrane. Basement membranes play an important role in tumor progression. In normal breast tissue, benign breast lesions and in situ NCL-COLL-IIp reacts with type II collagen and does not cross-react with malignancies, the basement membrane always surrounds ducts and tubules collagen types I, III, IV, V, VI, other human serum proteins or non-collagenous whereas in invasive breast carcinomas it is often absent. Collagen type VII is extracellular associated proteins. reported to be a defective membrane component in the condition Recessive Dystrophic Epidermolysis Bullosa (RDEB).
Human chondrosarcoma: immunohistochemical staining for collagen type II using NCL-COLL- IIp. Note diffuse staining of chondroid tissue. Paraffin section. Human skin: immunohistochemical staining for collagen type VII using NCL-COLL-VII. Note staining of the basal lamina of the stratified epithelium. Frozen section.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. auenurpiso oemro n lsaclso h osl lymph tonsil, the antigen. CPP32 of express to cells reported plasma also and are and marrow colonic bone megakaryocytes, marrow and bronchial, bone node osteocytes, collecting of and bone and neutrophils tubules Chondrocytes, thymus mature proximal epithelia. the renal duct of skin, salivary cells of epithelioreticular cells to kD epithelial reported ducts, is in is 32 CPP32 (SREBP). found which inactive protein actin form be binding (PARP), an element active polymerase regulatory (ADP-ribose) its cell as sterol poly to and of programmed synthesized cleavage apoptosis CPP32 of the during is for and residues. mediator processed responsible CPP32 apoptosis control is acid cells. and a in aspartic proenzyme as mammalian implicated act in of been to death side have thought family is C-terminal (caspase-3) this the of specifically beta- at Members which interleukin-1 proteases substrates the mammalian of of cleaves member family a (ICE) enzyme is converting (CPP)-32 protein protease Cysteine NCL-CPP32 lyophilized mL 0.1 mL, 1 JHM62 Clone (Caspase-3) CPP32 section. Paraffin walls. vessel and myocardium necrotic partially of using staining C9 Note component NCL-CCC9. complement for staining immunohistochemical myocardium: Human by or histochemistry necrosis. band enzyme contraction as of quantification such of been heart technique of elaborate the has Detection the of conventional necropsy. C9 with examination unreliable at complement of be component damage methods can damage complement myocardial diffuse synthesize or acute of infarction of myocardial detection of cases and and implications The in Male the forming reported unclear. cells. although express T is membrane, receptors, cytotoxic and this tissues in cell proteins binding found the components, protein reproductive forming into way pore female similar a a itself conform- in perforin, acts a inserts to C9 undergoes component Complement it and channels. binding, transmembrane protein After change final the complex. as attack ational complex membrane C5b-8 the the to of binds C9 component Complement NCL-CCC9 lyophilized mL 0.1 mL, 1 10A6 Clone C9 Component Complement (HIER) P HE)W (HIER) P el xrsigccisA n aebe hw ohv increased have to shown been have E be and skin cell to B status. proliferative A, proliferative of squamous reported fractions cyclins elevated various and is with expressing reported cells tumors keratosis A and breast of seborrhoeic Cyclin majority epidermis The may carcinoma. psoriasis, proliferation. human and binds including of phase in normal diseases kD, marker G2 detectable in is to a 60 A progression expressed as Cyclin of cycle activation. useful cell protein enzyme prove during to a leading increasing cdc2/cdk1 phase, A2, and phase, phase S M G2 as to to S G2 in defined cdk2, in kinase, commonly cdc-related and cycle. a cell with to more the independently associated of are stages A, different and at Cyclin abundance (cdk) kinases in dependent vary cyclin activate that proteins are Cyclins A NCL-CYCLIN lyophilized mL 1 6E6 Clone A Cyclin oectpamcsann ftmrcls aafnsection. Paraffin cells. B1. tumor NCL-CYCLIN of using staining B1 cytoplasmic cyclin Note for staining immunohistochemical carcinoma: breast Human staining. optimal produces fixation methacarn that note Please in cells. staining, positive Information Ki67 cytoplasmic Specific of Product subset by a represent indicated to reported cells, B2 are cyclin tissue positive proliferating before B concentration B1 in Cyclin cyclin peaking with expression. cycle and cell of earlier the subunits of detected is, period regulatory expression expression short as B specific A, a Cyclin to transition. cyclin restricted phase to therefore, M way to G2 similar the a affecting in p34/cdc2/cdk1 acts protein B Cyclin B1 NCL-CYCLIN lyophilized mL 0.1 mL, 1 7A9 Clone B1 Cyclin section. Paraffin cells. epithelial crypt of proportion Note small A. a NCL-CYCLIN of using staining A nuclear Cyclin intense for staining immunohistochemical colon: human Normal W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence HE)WC W (HIER) P HE)WC W (HIER) P O E te applications Other lcrnmicroscopy Electron /93
Primary Antibodies Cyclin D1 Cyclin E
Clone P2D11F11 Clone 13A3 1 mL, 0.1 mL lyophilized 1 mL, 0.1 mL lyophilized NCL-CYCLIN E F P (HIER) W NCL-CYCLIN D1-GM P (HIER/Enzyme) W Cyclin E was identified as a protein which would complement cyclin 1 mL liquid NCL-L-CYCLIN D1-GM P (HIER/Enzyme) W mutations in yeast and mammalian cells. Overexpression of cyclin Reference Range E shortens the length of the G1 phase, accelerating progression of the cell cycle into S phase. The activity of cyclin E is mediated through its activation 7 mL ready-to-use RTU-CYCLIN D1-GM P (HIER/Enzyme) of cyclin dependent kinase 2 (cdk2) protein and is modulated by the presence of cyclin dependent kinase inhibitors such as p16. Clone DCS-6 1 mL lyophilized NCL-CYCLIN D1 F P (HIER/Enzyme) W The D-type cyclins are a family of proteins which function primarily by regulating the activity of cyclin dependent kinases in the G1 phase of the cell cycle. Cyclin D1, a protein of 36 kD, is also known as PRAD1 or bcl-1. Maximum expression of cyclin D1 occurs at a critical point in mid to late G1 phase of the cell cycle. The cyclin D1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphomas due to the chromosomal translocation t(11;18). It has also been observed in approx- imately 30 percent of breast cancers.
Western blot: detection of human cyclin E (50 kD) using NCL-CYCLIN E. Lane A, molecular weight markers. Lane B, thymidine blocked MDA-MB-157 cell line immunoblotted with NCL-CYCLIN E.
Cyclin G Primary Antibodies
Clone 11C8 1 mL lyophilized NCL-CYCLIN G P (HIER) W Human breast carcinoma: immunohistochemical staining for cyclin D1 using NCL-CYCLIN D1-GM. Note nuclear staining of a proportion of tumor cells. Paraffin section. Cyclin G, a member of the cyclin family, is one of a number of proteins which is a transcriptional target of the tumor suppressor, p53. Cyclin G appears to be upregulated from early G1 to G1/S phase and is reported to be constitutively expressed throughout the cell cycle in T and B cell lines. In contrast, in stimulated peripheral T cells, cyclin G mRNA levels are highest Cyclin D3 in early G1 phase and decline during cell cycle progression. Cyclin G expression levels parallel p53 protein expression in murine B lymphocytes, Clone DCS-22 however, in several human Burkitt lymphomas and tissues of p53 null mice, 1 mL lyophilized NCL-CYCLIN D3 F P (HIER) W cyclin G expression levels can be both inverse to that of p53 levels or expressed independently of p53 protein. In damaged neurons, an increase in The 34 kD cyclin D3 protein shares 53 percent sequence homology with cyclin G mRNA expression has been shown in the early stages of nerve cyclin D1. Cyclin D3 expression is reported to be induced later than cyclin D1 regeneration and in situ hybridization has demonstrated cyclin G expression in G1 phase of the cell cycle. When complexed with cyclin dependent in a restricted group of mature neurons, particularly in the telencephalon kinases, cyclin D3 shows activity characteristic of other D-type cyclins. and the thalamus. This constitutive expression in some cell types suggests However, an increase in cyclin D3 expression with an absence of kinase that cyclin G may have a function different from other members of the cyclin activity has been observed in terminally differentiated, quiescent cells, group and that cyclin G expression is not predominantly regulated by p53 suggesting an additional role for cyclin D3. protein.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. C-K.Nt nes yolsi tiigo uo el.Prfi section. Paraffin cells. tumor of staining using cytoplasmic 1 intense cytokeratin Note for NCL-CK1. staining immunohistochemical carcinoma: cell squamous Human epithelium. squamous with reacts NCL-CK1 individually been 68 Information Specific of Product have weight of epithelium. molecular complex peptides in a class present has is cytokeratin and 1 complex kD Cytokeratin At human kD. catalogued. a 70 and different to characterized are 40 from 20 Keratins ranging weights least cytoskeletal molecular composition. with other filaments protein filament intermediate from of basis and the distinguished in on microfilaments diameter are and present microtubules cells as components such structures mammalian cytoskeletal all distinctive virtually filaments, Intermediate 34 Clone 1 Cytokeratin cell prostatic regions. cortex squamous of frontal the cases oesophageal in upregulated most be is of to cyclooxygenase-2 in reported of proportion as expression disease, well Alzheimer's high In as adenocarcinoma. a adenocarcinomas epithelial in and It both carcinomas adenocarcinomas. expressed in colon expressed and also the polyps is in is adenomatous It and of propria. cells cells is lamina interstitial epithelial and the cyclooxygenase-2 surface of crypts, colitis, cells upper ulcerative mononuclear the and in are expressed disease carcinomas strongly cell Crohn's basal In whereas differentiated negative. from cyclooxygenase-2 derived express carcinomas cell epidermis Squamous skin. normal a keratino- of suprabasal plays cytes differentiated, and more the angiogenesis in for of increases staining cyclooxygenase cyclooxygenase-2 inducer Immunohistochemical receptor differentiation. an cell keratinocyte is normal T in of It by role blood. and peripheral radiation form in to UV triggering response including stimuli, in mitogen-inducible inflammatory expressed various is a which synthase) (prostaglandin-endoperoxide is Cyclooxygenase-2 NCL-COX-2 lyophilized mL 0.1 mL, 1 4H12 Clone Cyclooxygenase-2 . LlohlzdNCL-CK1 lyophilized mL 0.5 B4 (HIER) P F (HIER) P C-K sacanseii nioy ti fpriua s ntecharacteri- the in epithelia. use complex particular certain of of is zation It antibody. chain-specific a is NCL-CK4 Information Specific carcinomas Product cell squamous epithelia. in stratified non-cornified be several cell to from bronchial reported and derived ciliated theis suprabasal cells cylindrical, 4 in of single Cytokeratin the and cells. in ducts in cells epithelial prostatic epithelium, expressed glands, superficial transitional sweat be bladder of the to groups urinary reported as the also of well is cells as 4 vagina, Cytokeratin anus tongue, and cornea. ectocervix, epiglottis, superficial esophagus, pharynx, the the of larynx, of that found cells as is epithelial It such protein. intermediate epithelium filament squamous intermediate cytokeratin non-cornifying kD in 59 a is 4 Cytokeratin NCL-CK4 lyophilized mL 0.5 6B10 Clone 4 Cytokeratin ee opg 5frteBn ed-oueformat. ready-to-use Bond the for 6. 25 cytokeratin page filament with to cross-reactive intermediate Refer not simplex. kD is It 58 bullosa 5. the cytokeratin for as epidermolysis biphasic known specific and protein epithelial is of most locus XM26 in at expressed Clone types be mesotheliomas. gene to reported 5 various also is cytokeratin 5 cause Cytokeratin the of in and can and mutations epiglottis 12q11-q13 Point vagina tongue, region. cell skin, urothelium, basal rectal-anal of in prostate, the carcinomas expressed of also cell of cells are squamous cells 6 basal epitheliomas, and basal 5 cells trachea, Cytokeratins luminal gland, mucosa. of skin, endocervical eg mammary of epithelia glands the basal epithelia sweat and to in of sebaceous squamous related as follicles, closely hair basic stratified well epidermis, is as and non-keratinizing that various mucosa, in 14 many protein tongue found are kD keratin in and 58 distribution acidic tissue proportions a similar share express is protofilaments. They II 6. 5 epidermis and cytokeratin type Cytokeratin tetramers human and 5. order (acidic) of cytokeratin higher I cells form type assemble Basal to Cytokeratins of cell. polypeptides composed epithelial (basic) each heteropolymers in obligatory member expressed epithelial one into is least in at family found that so each proteins pairs of in cytoskeletal synthesized of coordinately are family They cells. large a are Cytokeratins PA0468 ready-to-use Bond mL 7 RTU-CK5 ready-to-use mL 7 NCL-L-CK5 liquid mL 1 NCL-CK5 lyophilized mL 0.1 mL, 1 XM26 Clone 5 Cytokeratin section. Paraffin epithelium. mucosal of NCL-CK4. staining using cytoplasmic 4 Note cytokeratin for staining immunohistochemical tonsil: Human W P F etr blotting Western Paraffin Frozen C I HE)W (HIER) P F lwcytometry Flow Immunofluorescence HE)W (HIER) P F (HIER) P F (HIER) P HE)W (HIER) P F Reference Range O E te applications Other lcrnmicroscopy Electron /95
Primary Antibodies Product Specific Information Where clone OV-TL 12/30 can produce unwanted staining of endothelial cells, clone RN7 does not stain these cell types. The choice of epitope retrieval, heat or enzyme, to provide the best result with clone OV-TL 12/30 should be determined by the user. Clones RN7, OV-TL 12/30 and LP5K react with the human cytokeratin intermediate filament protein (54 kD) identified as cytokeratin 7. Refer to page 25 for the Bond ready-to-use format.
Human prostate: immunohistochemical staining for cytokeratin 5 using NCL-L-CK5. Note intense cytoplasmic staining of myoepithelial cells. Paraffin section.
Cytokeratin 6
Clone LHK6B 1 mL lyophilized NCL-CK6 F Infiltrating lobular carcinoma of breast: immunohistochemical staining for cytokeratin 7 antigen using NCL-L-CK7-560. Note intense membrane and cytoplasmic staining of malignant Cytokeratins are precisely regulated in tissue and little is known about the cells. Paraffin section. molecular mechanisms underlying this regulation. However, the expression pattern of cytokeratin 6 is known to be particularly complex. It is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyperproliferative situations. Epidermal injury results in activation of keratinocytes which produce and respond to growth Cytokeratin 8 factors and cytokines and become migratory. Activated keratinocytes express a specific pair of cytokeratins, 6 and 16. Furthermore, cytokeratin 6 Clone TS1
Primary Antibodies is reported to be expressed in approximately 76 percent of head and neck squamous cell carcinomas. The expression of cytokeratin 6 has been 1 mL, 0.1 mL lyophilized NCL-CK8-TS1 F P (HIER) particularly associated with differentiation. 1 mL liquid NCL-L-CK8-TS1 F P (HIER) Product Specific Information 7 mL ready-to-use RTU-CK8-TS1 F P (HIER) NCL-CK6 reacts with the human cytokeratin intermediate filament protein 7 mL Bond ready-to-use PA0567 P (HIER) (56 kD) identified as cytokeratin 6. Cytokeratin 8, also known as tissue polypeptide antigen (TPA), together with cytokeratin 18, is one of the first cytokeratins expressed in the embryo and persists in adult tissues. Both cytokeratins, 8 and 18, are major components of all simple epithelia but not of stratified squamous epithelia. Cytokeratin 8, Cytokeratin 7 reported to be expressed in the adenocarcinomas of individuals, is also found to be present in their sera. Clone RN7 Product Specific Information 1 mL, 0.1 mL liquid NCL-L-CK7-560 P (HIER) Reference Range Clone TS1 reacts with human cytokeratin intermediate filament protein 7 mL Bond ready-to-use PA0942 P (HIER) (52.5 kD) identified as cytokeratin 8. Refer to page 26 for the Bond ready-to-use format. Clone OV-TL 12/30 1 mL lyophilized NCL-CK7-OVTL F P (HIER/Enzyme) W 1 mL liquid NCL-L-CK7-OVTL F P (HIER/Enzyme) W 7 mL ready-to-use RTU-CK7-OVTL F P (HIER/Enzyme) Clone RN7 was developed to produce superior staining on paraffin sections. Cytokeratins are intermediate filament proteins present in epithelial cells. They are expressed in a tissue-specific manner in normal organs and the tumors that arise from them. Cytokeratin 7 belongs to the neutral basic type B subfamily of cytokeratins. Its distribution is confined to glandular and transitional epithelia. Cytokeratin 7 is reported to be expressed in abun- dance in cultured bronchial and mesothelial cells but only at lower levels in Human pulmonary adenocarcinoma: immunohistochemical staining for cytokeratin 8 using cultured epidermal cells. The predicted amino acid sequence of this keratin RTU-CK8-TS1. Note cytoplasmic staining of malignant cells. Paraffin section. has revealed a striking difference between this keratin and the type II keratins expressed in epidermal cells. Cytokeratin 7 has been reported in adenocarcinomas of the lung, breast, endometrium, ovary, thyroid as well as in carcinomas of the bladder and chromophobe renal cell carcinoma. Cytokeratin 7 and Cytokeratin 20 expression have been reported to show characteristic patterns on primary and metastatic lung and colorectal adenocarcinomas.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. 5 D dniida yoeai 14. protein cytokeratin filament as intermediate identified cytokeratin kD) human (50 the with reacts LL002 be Clone to reported Information been Specific has Product origin. 14 cell squamous Cytokeratin of types types. neoplasms cell in epithelial cell expressed stratified epithelial distinguish to simple useful from are 5 and 14 Cytokeratins NCL-L-LL002 liquid mL 1 NCL-LL002 lyophilized mL 0.1 mL, 1 LL002 Clone 14 Cytokeratin kD) (54 protein filament intermediate acidic 13. the cytokeratin as identified with reacts NCL-CK13 Information Specific Product the epidermis. canal keratinised of of anal layer esophagus, carcinomas cells basal tongue, the reserve in ectocervix, and glands, bladder, expressed salivary glands, non- glands, be endocervical sweat squamous, of to eccrine of reported and apocrine is component trachea, epithelium It major pseudostratified myoepithelium. a epithelium, and as transitional epithelium, expressed keratinised is 13 Cytokeratin NCL-CK13 lyophilized mL 0.5 KS-1A3 Clone 13 Cytokeratin protein filament 10. cytokeratin intermediate as cytokeratin identified kD) human (56.5 the with reacts NCL-CK10 Information Specific Product ano-rectal the of carcinoma cell in squamous expressed region. and be breast to of reported and carcinoma is epidermis 10 ductal sole Cytokeratin foot locations. canal, other anal of found the epidermises stratified is including 10 epithelia, Cytokeratin normal more keratinizing various carcinomas. in in squamous expressed of of some be layers of to suprabasal layers areas reported in differentiated is cells of suprabasal and number epithelia variable stratified in a non-keratinizing in found found also is It is epithelia. 10 Cytokeratin NCL-CK10 lyophilized mL 1 LHP1 Clone 10 Cytokeratin (HIER) P F (Enzyme) P F HE)W (HIER) P F (HIER) P F Reference Range nes yolsi tiigo aa el n arflils aafnsection. Paraffin follicles. hair and cells Note basal NCL-CK15. of using staining 15 cytoplasmic cytokeratin intense for staining immunohistochemical skin: human Normal are carcinomas reported cell are 15. cytokeratin carcinomas basal express cell to Squamous II percent not 15. type stem cytokeratin five natural follicle express a twenty to have hair reported to approximately from appear and not derived does trichoepitheliomas, cells, and in All keratin and I only partner. type epidermis expressed expression a fetal protein is epithelium, It filament squamous nail. intermediate fetal stratified kD of 52 keratinocytes a basal is 15 Cytokeratin NCL-CK15 lyophilized mL 0.1 mL, 1 LHK15 Clone 15 Cytokeratin ee opg 6frteBn ed-oueformat. ready-to-use Bond the for 26 page to Refer protein filament 17. cytokeratin intermediate as cytokeratin identified kD) human (46 the with reacts NCL-CK17 in expressed Information be cavity. Specific oral the to Product and cervix reported sweat in lung, is the and of 17 epithelium glands carcinomas cytokeratin cell salivary pseudostratified squamous tissue, in neoplastic of cells In myoepithelial cells glands. of bronchi, cells basal larynx, basal in eg trachea, expressed be epithelia to reported is complex 17 cytokeratin tissues normal In PA0114 ready-to-use Bond mL 7 NCL-CK17 lyophilized mL 0.1 mL, 1 E3 Clone 17 Cytokeratin protein filament 16. cytokeratin intermediate as cytokeratin identified kD) human healing, (48 the wound with reacts including NCL-CK16 states, Information pathological Specific Product various reported in carcinomas. are certain 6 and found and psoriasis undergoing 16 are be Cytokeratins region. keratinocytes suprabasal to where the expressed in are turnover rapid 6 and 16 Cytokeratins NCL-CK16 lyophilized mL 1 LL025 Clone 16 Cytokeratin W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence (HIER) P F (HIER) P (HIER) P F HE)W (HIER) P F New! O E te applications Other lcrnmicroscopy Electron /97
Primary Antibodies Human squamous cell carcinoma, floor of the mouth: immunohistochemical staining for Human rectal adenocarcinoma: immunohistochemical staining for cytokeratin 19 using cytokeratin 17 using NCL-CK17. Note cytoplasmic staining of malignant cells. Paraffin section. NCL-CK19. Note cytoplasmic staining of malignant epithelial cells. Paraffin section.
Cytokeratin 18 Cytokeratin 20
Clone DC-10 Clone PW31 1 mL, 0.1 mL lyophilized NCL-CK18 F P (HIER) 1 mL, 0.1 mL liquid NCL-L-CK20-561 P (HIER) Reference Range Cytokeratin 18 is normally co-expressed with cytokeratin 8 and is found in 7 mL Bond ready-to-use PA0918 P (HIER) most simple ductal and glandular epithelia. Product Specific Information Clone CK205 1 mL lyophilized NCL-CK20-543 P (HIER) NCL-CK18 reacts with the acidic cytokeratin intermediate filament protein (45 kD) identified as cytokeratin 18. Cytokeratin 18 is reported not to be expressed s in stratified squamous epithelium on most squamous cell carcinomas. Clone K 20.8 1 mL lyophilized NCL-CK20 P (HIER/Enzyme) W
Primary Antibodies 1 mL liquid NCL-L-CK20 P (HIER/Enzyme) W 7 mL ready-to-use RTU-CK20 P (HIER/Enzyme) Clone PW31 was developed to produce superior staining on paraffin sections. Cytokeratin 20 has been demonstrated to be almost entirely confined to the gastric and intestinal epithelium, urothelium and Merkel cells of the skin. Cytokeratin 20 is less acidic than other type I cytokeratins and is of interest due to its restricted tissue expression. In normal tissue, cytokeratin 20 is expressed in intestinal epithelium, gastric foveolar epithelium, a number of endocrine cells in the upper portions of the pyloric glands, urothelium and Merkel cells in epidermis. In tumors it is reported, there is a marked Human colonic adenocarcinoma: immunohistochemical staining for cytokeratin 18 using difference in the expression of cytokeratin 20 within different carcinomas. NCL-CK18. Note cytoplasmic staining of malignant epithelial cells. Paraffin section. Neoplasms expressing cytokeratin 20 are derived from normal epithelia which themselves expressed cytokeratin 20. Colorectal carcinomas consis- tently express cytokeratin 20, while gastric adenocarcinomas express cytokeratin 20 to a lesser degree. Adenocarcinomas of the gall bladder and bile duct, ductal cell adenocarcinomas of the pancreas, mucinous ovarian Cytokeratin 19 tumors, Merkel cell tumors and transitional cell carcinomas have also been reported to express cytokeratin 20. Clone b170 Refer to page 26 for the Bond ready-to-use format. 1 mL, 0.1 mL lyophilized NCL-CK19 F P (Enzyme) 7 mL Bond ready-to-use PA0799 P (Enzyme) The smallest human cytokeratin filament protein (40 kD) has been identified as cytokeratin 19 and has been reported to be expressed in a large number of epithelial cell types, including many ductal and glandular epithelia. Product Specific Information NCL-CK19 produces a complex heterogenous staining pattern in non- keratinizing squamous epithelia and hair follicles, with strong staining of the basal layer observed. Refer to page 26 for the Bond ready-to-use format. Human colonic adenocarcinoma: immunohistochemical staining for cytokeratin 20 using NCL-L-CK20-561. Note staining of malignant cells. Paraffin section.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. oeitnectpamcsann fdca pteilcls aafnsection. Paraffin cells. epithelial ductal NCL-L-5D3. of using staining 18 cytoplasmic and intense 8 Note cytokeratins for staining immunohistochemical breast: Human format. ready-to-use Bond the for 27 page 18, to Refer and Journal (1987)). al. 8 377-384 et B 153: (Angus Pathology. cytokeratins CAM5.2 of clone to specificities as similar shares 5D3 identified Clone kD, Information 45 Specific Product and kD squamous 52.5 respectively. filament keratinizing cell of intermediate cytokeratin squamous from human proteins with most absent reacts and 5D3 are Clone adenocarcinomas carcinomas. been cytokeratins have in These they all expressed tissues, in carcinomas. be expressed neoplastic be in to to and reported reported epithelium are glandular 18 and and 8 simple cytokeratins tissues, normal In PA0067 ready-to-use Bond mL 7 RTU-5D3 ready-to-use mL 7 NCL-L-5D3 liquid mL 1 NCL-5D3 lyophilized mL 0.1 mL, 1 5D3 Clone (8/18) Cytokeratin section. Paraffin cells. epithelial using of 18 staining and cytoplasmic 5 intense cytokeratins Note for NCL-C50. staining immunohistochemical bowel: small fetal Human be may NCL-C50 using sections paraffin the on However, respectively. 5 18 variable. and cytokeratin 5 of cytokeratins as 58 recognition identified of kD, proteins 45 filament and intermediate kD cytokeratin human with reacts be NCL-C50 to reported Information is epithelium. Specific stratified Product a 5 of cells and Cytokeratin suprabasal cells and tumors. cells epithelial basal simple epithelial-derived by expressed by of expressed be range to wide reported is 18 Cytokeratin NCL-C50 lyophilized mL 1 C-50 Clone (5/18) Cytokeratin Ezm)C (Enzyme) P F (HIER) P F (Enzyme) P F (HIER) P Ezm)C (Enzyme) P F LBn ed-ouePA0909 ready-to-use Bond mL 7 RTU-AE1/AE3 ready-to-use mL 7 NCL-L-AE1/AE3 liquid mL 1 NCL-AE1/AE3 lyophilized mL 0.1 mL, 1 cocktail AE3 Clone AE1, Clone Multi Cytokeratin, variable. sections paraffin be on may 18 LP34 cytokeratin clone of using recognition 6 The 5, tissue. proteins frozen on filament 18 intermediate and cytokeratin less human with reacts and LP34 Clone origin epithelial Information Specific beenof Product lymphomas cells as have cytokeratins. such these tumor tumors cytokeratins express not Non-epithelial in do origin. These ectodermal, mesothelial expressed stratified of origin. are be commonly to that in to epithelia cells endodermal of reported epithelial glandular range or broad include simple a mesodermal, These in from expressed epithelia. be tissues, to squamous reported epithelial are 18 human and 6 5, Cytokeratins RTU-LP34 ready-to-use mL 7 NCL-L-LP34 liquid mL 1 NCL-LP34 lyophilized mL 1 LP34 Clone (5/6/18) Cytokeratin e loMliCtkrtn(////01/8 npg 137. (4/5/6/8/10/13/18). page on (4/5/6/8/10/13/18) Multi-Cytokeratin also See NCL-C11 lyophilized mL 1 C-11 Clone (4/5/6/8/10/13/18) Multi Cytokeratin, 137. page on 1/5/10/14 Multi-Cytokeratin also See PA0134 ready-to-use Bond mL 7 RTU-CK34BE12 ready-to-use mL 7 NCL-CK34BE12 lyophilized mL 0.1 mL, 1 34 Clone (1/5/10/14) Multi Cytokeratin, 136. page on Multi-Cytokeratin also See W P F etr blotting Western Paraffin Frozen E12 C I Ezm)C (Enzyme) P F (HIER) P F lwcytometry Flow Immunofluorescence Ezm)C (Enzyme) P F (HIER) P F (Enzyme) P F (Enzyme) P (HIER) P F (Enzyme) P (HIER) P F (HIER) P F HE)W (HIER) P F O E te applications Other lcrnmicroscopy Electron /99
Primary Antibodies Cytokeratin, Multi (5/6/8/18) Daxx
Clone 5D3, Clone LP34 cocktail Clone 36H11 1 mL, 0.1 mL lyophilized NCL-CK5/6/8/18 F P (Enzyme) 1 mL lyophilized NCL-DAXX P (HIER) 1 mL liquid NCL-L-CK5/6/8/18 F P (Enzyme) Daxx binds the death domain of Fas and links this receptor to an apoptosis 7 mL ready-to-use RTU-CK5/6/8/18 F P (Enzyme) pathway involving the activation of Jun N-terminal kinase (JNK). The human homolog of Daxx enhances Fas-mediated apoptosis probably by modulating See also Multi-Cytokeratin (5/6/8/18) on page 137. the transcription of genes involved in Fas-induced caspase activation and apoptosis. The Fas-binding domain of Daxx is a dominant negative inhibitor of both Fas-induced apoptosis and JNK inactivation, while the FADD death domain partially inhibits death but not JNK activation. The Daxx apoptotic pathway is sensitive to both bcl-2 and dominant negative JNK pathway Cytomegalovirus Antibodies components and acts cooperatively with the FADD pathway. Therefore, Daxx and FADD define two distinct pathways downstream of Fas. Daxx Clone 2, Clone 6 cocktail mRNA is widely expressed in human tissues such as heart, brain, lung, liver, 1 mL, 0.1 mL lyophilized Cytomegalovirus (pp65 antigen) skeletal muscle, kidney, pancreas and placenta. The human Daxx gene has NCL-CMVpp65 P (HIER) W I been mapped to 6p21.3 in the major histocompatibility complex (MHC) region. Its location may help in understanding the genetic basis of auto- Clone QB1/42 immune diseases. In cells, the protein is found in the nucleus and to a lesser extent in the cytoplasm. 1 mL lyophilized Cytomegalovirus (early antigen) NCL-CMV-EA F P (HIER) Clone QB1/06 1 mL lyophilized Cytomegalovirus (late antigen) NCL-CMV-LA F P (HIER) Cytomegalovirus (CMV) is an opportunistic pathogen infecting lung, kidney, gut and other organs in situations where an individual is immunologically
Primary Antibodies immature, such as the fetus and neonate. Infection also occurs in immuno- suppressed individuals eg transplant recipients, individuals undergoing chemotherapy and those with HIV infection. The typical course of an active CMV infection in the immunosuppressed individual is reported to be Human breast carcinoma: immunohistochemical staining for Daxx using NCL-DAXX. Note characterized by a period of pp65 antigenaemia which correlates with viral cytoplasmic and occasional nuclear staining. Paraffin section. replication. This may be observed over some weeks and begins before the onset of clinical symptoms. Following the isolation of CMV strains in cell culture, early viral proteins are expressed in the cell nucleus, within 3 to 24 hours of infection. After 48 to 72 hours, a number of late viral proteins may be demonstrated in the nucleus and cytoplasm of infected cells. DEC-205 (CD205) Product Specific Information Clone 11A10 NCL-CMVpp65 is a pool of 2 unique monoclonal antibodies suitable for the detection of the pp65 antigen in cytospin preparations. 1 mL liquid NCL-L-DEC205 P (HIER) P (HIER) See also CD205 (DEC-205) on page 88.
Antigenaemia positive peripheral polymorphonuclear leukocytes: immunofluorescence for Cytomegalovirus pp65 antigen using NCL-CMVpp65. Note characteristic nuclear staining. Formalin/sucrose-fixed cytospin preparation.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. eotdi acetcitautlpplaymcnu epam (IPMN) carcinomas. ductal neoplasms from histologically tumorigenesis mucinous in is is differences papillary protein genetic suggests intraductal DPC4 it and of pancreatic the expression when in to continued The cancer reported leads carcinoma. which pancreatic a as progression infiltrating recognizable DPC4 neoplastic of of the Loss of in carcinomas. development pancreatic late percent specific in occurs and alterations 50 sensitive expression a gene be in DPC4 to for reported The reported been marker myelogenous cancers. has breast protein is acute DPC4 and of of colon gene expression ovarian, subset carcinomas, DPC4 a tract biliary and the leukemias, adenocarcinomas of ductal deletion pancreatic to or nucleus Smad3 activation. Mutation the transcriptional and to and DNA-binding binds sequence-specific translocate in that Smad2 participate which cofactor complexes receptor-activated a transcriptional as factor-beta generating acts metastatic Smad4, gene and growth as pancreatic suppressor known in tumor transforming also deleted a DPC4, or is cancers. mutated (DPC4) colon frequently 4 be locus to cancer reported pancreatic in Deleted NCL-DPC4 lyophilized mL 1 JM56 Clone Protein 4 Locus Cancer Pancreatic in Deleted epithelial malignant of section. staining Paraffin cytoplasmic cells. granular Note NCL-DCC. colorectal using in protein deleted cancer for staining immunohistochemical adenocarcinoma: colonic Human carcinomas reported. these colorectal is and in protein tumors DCC colorectal reduced astrocytic of is be In expression protein percent. reduced of to 50 DCC and to reported of cancers 36 percent expression by been brain The cancers also and 70 leukemias. prostatic some has in breast, than gene also gastric, DCC pancreatic, more allele- The in or transcripts. inactivated in transcripts of of occur loss loss splicing allelic specific aberrant of to mutations, result the localized reported be carcinomas, may is expression DCC Altered which cells. to particularly crypts, goblet cell the the the or in to in staining cell restricted cytoplasmic to is granular expression cell intense with DCC of tissues mucosa colon, regulation epithelial normal the most In it in in interaction. developing participate where expressed substratum the may be systems of protein to guidance the reported nervous the is where peripheral be gene for DCC can required and The receptor protein central axons. netrin this the a of as of expression functions axons reported in highest structurally The found protein NCAM. transmembrane a to a is encodes 18 similar chromosome that on gene located suppressor gene (DCC) tumor cancer colorectal in deleted The NCL-DCC lyophilized mL 1 DM51 Clone Protein Cancer Colorectal in Deleted (HIER) P (HIER) P ee opg 7frteBn ed-oueformat. ready-to-use Bond the for 27 page to giving Refer muscle cardiac and skeletal in appearance. bands striated cells. Z to characteristic muscle the a smooth appear to and confined not cardiac) is and labeling does (skeletal DE- The striated Clone antibody both tissues, The with normal reacts In cells. RII proteins. filament muscle filament intermediate intermediate in the other kD) of recognize piece (53 rod desmin kD 18 protein an with reacts NCL-DES-DERII Information Specific PA0032 Product ready-to-use Bond mL 7 RTU-DES-DERII ready-to-use mL 7 NCL-L-DES-DERII liquid mL 1 NCL-DES-DERII lyophilized mL 0.1 mL, 1 DE-R-11 Clone Desmin C--O-.Nt nes ebaeadctpamcsann ftmrcls Paraffin cells. tumor of staining section. cytoplasmic and membrane using intense DOG-1 Note for NCL-L-DOG-1. staining immunohistochemical tumor: stromal gastrointestinal Human format. ready-to-use Bond the for 27 staining. page background to increased Refer in result may diluents PBS-based of use The the in Information aid Specific Product antigen. to Receptor KIT express Factor suggested to Growth fail been that Platelet-Derived mutants has Alpha including DOG-1 GISTs, KIT of all for including identification GISTs, Reactivity to scorable of has GISTs. due expressed percent DOG-1 97.8 which, negative in for is tumors, found Immunoreactivity be tissue to GISTs. function, reported soft with been other confused unknown in be can expressed tumors of appearance, stromal rarely gastrointestinal protein is of and acid membrane (GISTs) plasma amino the on 986 predominantly a DOG-1, PA0219 ready-to-use Bond mL 7 NCL-L-DOG-1 liquid mL 0.1 mL, 1 K9 Clone DOG-1 externa. muscularis the in section. cells Paraffin muscle of staining using cytoplasmic desmin Note for NCL-DES-DERII. staining immunohistochemical intestine: small human Normal W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence Ezm)W (Enzyme) P F (HIER) P (HIER) P (HIER) P (Enzyme) P F Ezm)W (Enzyme) P F Reference Range O E te applications Other lcrnmicroscopy Electron 101 /
Primary Antibodies Dysferlin
Clone Ham1/7B6 1 mL, 0.1 mL lyophilized NCL-Hamlet FPW Clone Ham3/17B2 1 mL, 0.1 mL lyophilized NCL-Hamlet-2 F P (HIER) W Dysferlin is the protein product of the 2p13 gene that is defective in individuals with Limb-Girdle Muscular Dystrophy type 2B (LGMD2B) and Miyoshi Myopathy (MM). Dysferlin is normally localized to the muscle plasma membrane. In individuals with LGMD2B and MM, immunoreactivity Human skeletal muscle: immunohistochemical staining for dystrophin using NCL-DYSA. Note to dysferlin is reported to be severely reduced or lost, depending on the type membrane staining of normal muscle fibers (A) and reduced and variable staining of muscle fibers in an individual with Duchenne and Becker muscular dystrophy (B). Paraffin section. of mutation. Individuals with other neuromuscular conditions demonstrate normal labeling patterns. Product Specific Information NCL-Hamlet may require heat induced epitope retrieval in some cases. E-Cadherin labeling with an antibody to beta-spectrin, to monitor membrane integrity, is an essential immunohistochemical control. Clone 36B5 1 mL, 0.1 mL lyophilized NCL-E-Cad P (HIER) 7 mL ready-to-use RTU-E-Cad P (HIER) Dystrophin Antibodies 7 mL Bond ready-to-use PA0387 P (HIER) E-cadherin is a Ca2+ -dependent, transmembrane cell adhesion molecule. It Clone Dy4/6D3 plays an important role in the growth, development and the intercellular 2.5 mL, 1 mL lyophilized Dystrophin (Rod Domain) adhesion of epithelial cells. Most tumors have an abnormal architecture and any subsequent loss of adhesiveness is thought to be an important step in NCL-DYS1 FWE the development of local invasion. E-cadherin may have a role in neoplastic Primary Antibodies progression, particularly as a suppressor of invasion. In prostate cancers, Clone Dy8/6C5 for example, the expression of E-cadherin is reported to be reduced or 2.5 mL, 1 mL lyophilized Dystrophin (C-terminus) absent in comparison with its expression in normal prostate which is NCL-DYS2 FWE uniformly strong. Reduced expression or absence of E-cadherin in addition to alpha, beta and gamma-catenin in primary breast carcinomas has also Clone Dy10/12B2 been reported and these four proteins are associated with the development of metastases.The main immunohistochemical utility of E-cadherin is to 2.5 mL, 1 mL lyophilized Dystrophin (N-terminus) highlight differential expression of this protein in lobular and ductal NCL-DYS3 FWE carcinomas. Refer to page 28 for the Bond ready-to-use format. Clone 13H6 1 mL lyophilized Dystrophin (C-terminus) NCL-DYSA P (HIER) Clone 34C5 1 mL lyophilized Dystrophin (N-terminus) NCL-DYSB P (HIER) Duchenne Muscular dystrophy (DMD) is the most severe of the muscular dystrophies resulting in progressive muscular wasting and death. Dystrophin is the 427 kD protein product of the DMD/BMD gene located on the X chromosome at position Xp2. Western blotting and immunohisto- chemistry are the two established methods for the detection of Human pulmonary adenocarcinoma: immunohistochemical staining for E-cadherin using abnormalities of dystrophin expression in muscle samples. NCL-E-Cad. Note intense cytoplasmic and membrane staining of tumor cells. Paraffin section. Product Specific Information NCL-DYS1, NCL-DYS2 and NCL-DYS3 map within amino acids 1181-1388, 3669-3685 and 321-494, respectively, on the dystrophin molecule. The immunolabeling patterns for NCL-DYS1, NCL-DYS2 and NCL-DYS3 are similar. NCL-DYSA is raised to a region of the dystrophin molecule, upstream from the C-terminal region and NCL-DYSB is raised to a region of the N-terminus of the dystrophin molecule. NCL-DYSA and NCL-DYSB will be of particular interest in the investigation of archived formalin-fixed, paraffin-embedded material. Labeling with an antibody to beta-spectrin, to monitor membrane integrity, is an essential immunohistochemical control.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. oeprncersann falcl uli aafnsection. Paraffin nuclei. cell all of NCL-EMERIN. staining using perinuclear emerin Note for staining immunohistochemical muscle: skeletal Human product. gene STA normal the of detection the in use of is NCL-EMERIN Information the highest Specific crucial Product in in a localized plays EDMD. found deficiency of is pathology its is the and Emerin in cells and part muscle. muscle kD and normal tissues cardiac 34 of serine-rich membrane in and tendons nuclear a skeletal ubiquitous encodes Achilles in locus, is Xq28 concentration which elbows, at gene, emerin, the STA protein, course The the disease. in in the early of distribution seen X-linked humero-peroneal contractures, with heart are muscles onset as postcervical progressing presented Contractures late usually slowly cardiomyopathy and block. a by muscle skeletal is of characterized wasting (EDMD) disorder dystrophy recessive muscular Emery-Dreifuss NCL-EMERIN lyophilized mL 0.1 mL, 1 4G5 Clone Emerin section. Paraffin wall. arterial the extracellular within Note NCL-ELASTIN. staining using elastin for staining immunohistochemical aorta: Human syndrome, Marfan's as in such found diseases elastin. is inherited syndrome. Bushke-Ollendorf to Thevessels the molecule and insoluble lead elastin elasticum states. blood pseudoxanthoma the to in mature disease in reported defects different tissue are Genetic the hypertension. and elastic may and which ageing of atherosclerosis form material with accumulation amorphous to morphology abnormal tropoelastin an its residues secreted, predominantly change is Once lysyl it space. Ultrastructurally, specific cross-linking an and extracellular modification into chemical of via organization the covalently its and joined in are to synthesized molecules prior fiber is modifications It elastic post-ribosomal a tissue. undergoes of which elastic (tropoelastin) number protein the single-chain vertebrate imparts soluble, a which to as tissue secreted elasticity connective in of found protein property polymeric a is Elastin NCL-ELASTIN lyophilized mL 0.5 BA-4 Clone Elastin (Enzyme) P HE)W (HIER) P F ebaesann fclsi ohteteaitraadteaetra aafnsection. Paraffin externa. theca and interna theca the both in Note cells NCL-CD105. of using staining endoglin membrane for staining immunohistochemical ovary: human Normal of presence the in cells positive CD105 of lysis monocyte the of in study complement. circulating and of the adhesion cells in of cellular interest blood of leukemia variety studies of myelocytic macrophages, a be and into in lymphoblastic may differentiation acute venules expression on ENDOGLIN and levels cells. arterioles low expressed at capillaries, be and of to tissues reported cells is antigen that endothelial proposed CD105 been on receptor. has recognition TGF-beta It a a family. is integrin is the endoglin membrane which of receptors homodimeric RGD adhesion sequence for endothelial motif peptide an the is containing Endoglin, glycoprotein as known also CD105, NCL-CD105 lyophilized mL 0.1 mL, 1 4G11 Clone (CD105) Endoglin ohv oei ati le eln n ntedvlpeto neural-crest of development the in gall and reported healing been diseased cells. ulcer derived also gastric and in has role ETA normal a cells. have placenta, to granulosa luteinized medial full-term ovarian in and arteries, ETA bladder of of presence muscle and the Studies smooth negative. reported colon was have atrium, liver immunohistochemistry whereas ventricle, lung, using duodenum, cerebellum, and cortex, aorta, glands cerebral adrenal the in kidney, in levels by levels moderate mRNA mediated high with of placenta Analysis reported are (ETB). blotting sub-type effects receptor Northern non-selective biological the receptor endothelin and Their isolated endothelin-1-selective (ETA) receptors, originally cells. different two peptides endothelial through vasoconstrictive vascular potent from are (ET) Endothelins NCL-L-ETA liquid mL 0.1 mL, 1 RJT24 Clone (ETA) Receptor Endothelin-1 CD34. about information further for 77 page to Refer PA0212 ready-to-use Bond mL 7 RTU-END ready-to-use mL 7 NCL-L-END liquid mL 1 NCL-END lyophilized mL 0.1 mL, 1 QBEnd/10 Clone (CD34) Marker Cell Endothelial W P F etr blotting Western Paraffin Frozen C I Ezm)C (Enzyme) P F lwcytometry Flow Immunofluorescence (Enzyme) P F (HIER) P (HIER) P Ezm)C (Enzyme) P F (HIER) P O E te applications Other lcrnmicroscopy Electron 103 /
Primary Antibodies Envoplakin
Clone CRENV-1 1 mL lyophilized NCL-ENVO F P (HIER) Envoplakin is a membrane-associated precursor of the epidermal cornified envelope which is a layer of transglutaminase cross-linked protein deposited under the plasma membrane of keratinocytes in the outermost layers of the epidermis. The envoplakin protein (210 kD) is expressed in keratinizing and non-keratinizing stratified squamous epithelia but not in simple epithelia or non-epithelia. The human envoplakin gene (EVPL) has Human skin, pyogenic granuloma: immunohistochemical staining for endothelin-1 receptor been localized to the region of the tylosis oesophageal cancer gene (TOCG) using NCL-L-ETA. Note membrane staining of the endothelial cells. Paraffin section. on 17q25 and is physically linked to D17S1603. This sequence-tagged site segregates with the autosomal dominant human disease focal non- epidermolytic palmoplantar keratosis which is associated with an increased risk of oesophageal cancer. This chromosomal localization of the envoplakin gene, the homology of the encoded protein to keratin-binding proteins and Enterovirus its expression in epidermal and oesophageal keratinocytes all raise the possibility that loss of envoplakin function could be responsible for this form Clone 5-D8/1 of palmoplantar keratoderma. 1 mL lyophilized enterovirus (unconjugated) NCL-ENTERO WIO Enteroviruses are a large family of viruses whose main site of infection is the alimentary tract. Dissemination via the bloodstream is the likely route of spread to the wide range of target organs susceptible to infection. Most enterovirus infections are subclinical in young children. However, they can cause a wide range of syndromes involving many of the body systems eg myocarditis, respiratory and neonatal diseases. Product Specific Information Primary Antibodies NCL-ENTERO recognizes an epitope on the VP1 peptide, which is highly conserved within the Enterovirus group, except for Hepatitis A virus. The Human tonsil: immunohistochemical staining for envoplakin using NCL-ENVO. Note mainly antibody reacts with most echovirus strains (except some strains of membrane staining of epithelial cells. Paraffin section. echovirus 22 and 23), Poliovirus and Enterovirus strains. No reaction is observed with tissue culture grown strains of Respiratory syncytial virus, Parainfluenza virus types 1, 2, 3 and 4b, Herpesvirus types 1 and 2, Influenza virus types A and B, Mumps virus, Measles virus, Varicella-zoster virus, Cytomegalovirus and negative tissue culture cells routinely used in virus Epidermal Growth Factor Receptor isolation. Clone EGFR.25 1 mL, 0.1 mL lyophilized (Cytoplasmic Domain) NCL-EGFR-384 F P (HIER) 1 mL liquid (Cytoplasmic Domain) NCL-L-EGFR-384 F P (HIER) Reference Range Clone EGFR.113 1 mL, 0.1 mL lyophilized (Extracellular Domain) NCL-EGFR F P (HIER) 1 mL liquid (Extracellular Domain) NCL-L-EGFR F P (HIER) Coxsackie B4 virus infected BW HEp cells: immunocytochemical staining for Coxsackie B4 virus using NCL-ENTERO. Note strong cytoplasmic staining of infected cells. Acetone-fixed Epidermal growth factor receptor (EGFR) is a transmembrane protein cells. receptor of 170 kD with tyrosine kinase activity. Increased levels of EGFR are reported to be linked with malignant transformation of squamous cells eg in squamous cell carcinoma of the lung, head, neck, skin, cervix and esophagus. EGFR may also play a role in the development and progression of hepatocellular carcinomas where recurrence rates are higher in EGFR- positive cases. This correlation has similarly been reported in colorectal cancers where EGFR, produced by tumor cells, plays an important role in the invasiveness and proliferation of colorectal cancers. The majority of published studies of EGFR expression in human breast cancer has similarly shown an association with EGFR expression where it is inversely related to estrogen receptor status. Product Specific Information Clone EGFR.25 is raised to the cytoplasmic domain of the EGFR molecule whereas clone EGFR.113 is raised to the extracellular domain.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. sn C-O-1 oemmrn tiigo uo el.Prfi sections. Paraffin cells. tumor of staining membrane Note antigen NCL-MOC-31. epithelial-related using for staining immunohistochemical adenocarcinoma: lung Human results best sheet. for data the that on described recommended as formalin- methods is on both try It patterns customers 0.01M (121°C) staining tissue. utilizing strongest cooker minutes) paraffin-embedded the 60 pressure fixed, for provides a (90°C (pH6.0) method either buffer bath citrate water that heated 626-630 shown a 48: been or Pathology. has has method Clinical testing antibody of Journal Cell this Further J, of Small (1995)). Oates and characteristic on C A Workshop (Edwards a Antigens. reported International to (SCLC) epithelial Second an Cancer assigned the with most Lung be react at on which to 2 determined present reported SCLC-Cluster antigen kD as is known 40 MOC-31 antibodies of of epithelia. group antigen malignant epithelial and an normal with reacts NCL-MOC-31 NCL-MOC-31 lyophilized mL 1 MOC-31 Clone Antigen Epithelial-Related to format. useful ready-to-use Bond the is for be 28 to page EMA to described Refer notably lymphomas. with also Hodgkin’s of is subset together EMA a in origin. expressed combin- epithelial antibodies the of whereas cells anti-cytokeratin origin characterize been lymphoid of has of cells It markers labelling with ation epithelia. in conjunction useful in neoplastic are used and when EMA (LCA) to normal CD45 to of to reported markers variety that is reported a episialin, in as known expressed also be (EMA), antigen membrane Epithelial PA0035 ready-to-use Bond mL 7 RTU-EMA ready-to-use mL 7 NCL-L-EMA liquid mL 1 NCL-EMA lyophilized mL 0.1 mL, 1 GP1.4 Clone Antigen Membrane Epithelial tumor of staining section. membrane Paraffin intense cells. Note NCL-L-EGFR-384. epidermal using for receptor staining factor immunohistochemical growth breast: of carcinoma cell squamous Human FP FP (HIER) P F (HIER) P FP pti-arvrs(al antigens) (early virus Epstein-Barr section. Paraffin only. cells epithelial of NCL-ESA. staining using membrane antigen Note specific epithelial for staining immunohistochemical appendix: Human is and cells epithelial is human It of glycoprotein. majority surface cells. the mesothelial cell in in kD expressed expressed rarely 40 be a to is reported (ESA) antigen specific Epithelial RTU-ESA ready-to-use mL 7 NCL-ESA lyophilized mL 0.1 mL, 1 VU-1D9 Clone Antigen Specific Epithelial hr al iu elcto sudra.Prfi-meddcells. Paraffin-embedded only underway. cells is infected replication of virus staining early intense where Note NCL-EADE31. antigen using early protein for non-structural staining diffuse immunocytochemical cells: B95-8 transformed virus Epstein-Barr using (pH6.0). cells buffer citrate RAJI with and (HIER) formalin-fixed, retrieval B95-8 epitope with induced EBV-transformed react heat eg NCL-EARF2 antigen and cells early NCL-EADE31 paraffin-embedded EBV kD). recognizes by NCL-EARF2 (85 encoded frame. restricted kD), reading 52 to open (50 BMRF-1 diffuse the antigen early EBV recognizes NCL-EADE31 their most of replication, antigen Information virus Specific early basis Product of The leukoplakia. the phase cell restricted. lytic hairy on of early and keratinocytes identified the in diffuse notably during been namely expressed non-structural have is cell, of diffuse antigen the group in early least At a distribution of replication. DNA are viral forms require not antigens two do which of early synthesis the (EBV) proteins, diffuse) virus antigen Epstein-Barr (early virus NCL-EADE31 Epstein-Barr lyophilized mL 1 G3-E31 Clone Reference Range W P F etr blotting Western Paraffin Frozen HE)WO W (HIER) P C I lwcytometry Flow Immunofluorescence (Enzyme) P F Ezm)W (Enzyme) P F O E te applications Other lcrnmicroscopy Electron 105 /
Primary Antibodies Epstein-Barr virus-Induced Gene 3 Epstein-Barr virus (nuclear antigen 2)
Protein Clone PE2 1 mL, 0.1 mL lyophilized NCL-EBV-PE2 FW Clone EL8 1 mL lyophilized NCL-EBI-3 F P (HIER) Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA2) is an EBV-encoded nuclear protein of 82 kD. EBNA2 is essential for growth transformation of Epstein-Barr virus (EBV)-associated Hodgkin's lymphoma (HL) and naso- B lymphocytes and has been shown to modulate the activity of several viral pharyngeal carcinoma (NPC) usually occurs in individuals without clinically and cellular promoters. apparent deficiencies in anti-viral immunity. Despite expressing viral proteins, both tumors are apparently able to escape EBV-specific immunity in vivo. EBI-3 is an EBV-induced cytokine homologous to the interleukin 12 p40 subunit which can heterodimerize with the interleukin 12 p35 subunit. Researchers have suggested that EBI-3 protein may function to antagonize Estrogen Receptor interleukin 12 and to inhibit the development of a Th1 immune response. It has been reported that EBI-3 protein is strongly expressed in Hodgkin's Clone 6F11 Reed Sternberg (RS) cells in approximately 96 percent of HL cases, independently of the EBV status of the tumor cells. EBI-3 protein has also 2 mL lyophilized NCL-ER-6F11/2 F P (HIER) W C been reported to be detected in a small percentage of epithelial tumor cells 2 mL liquid NCL-L-ER-6F11/2 F P (HIER) W C of NPC biopsies but not in Burkitt's lymphomas. EBI-3 protein may be an 1 mL. 0.1mL lyophilized NCL-ER-6F11 F P (HIER) W C additional component of the repertoire employed by Hodgkin's RS cells to Reference Range inhibit and effect anti-tumor or anti-viral immune response. EBI-3 protein 1 mL liquid NCL-L-ER-6F11 F P (HIER) W C expression has also been reported in spleen, tonsil, mature dendritic cells, 7 mL ready-to-use RTU-ER-6F11 F P (HIER) W colonic mucosa and at high levels in full term placenta. 7 mL Bond ready-to-use PA0151 P (HIER) Estrogen receptor (ER) content of breast cancer tissue is an important parameter in the prediction of prognosis and response to endocrine therapy. The introduction of highly specific monoclonal antibodies to ER has allowed the determination of receptor status of breast tumors to be carried out in routine histopathology laboratories.
Primary Antibodies Product Specific Information Clone 6F11 is raised to the full length alpha form of the estrogen receptor molecule present on human ER antigen, located in the nucleus of ER positive normal and neoplastic cells. Clone 6F11 has been extensively tested (Bevitt D J et al. Journal of Pathology. 183 : 228-232 (1997)). Further publications exist that discuss the sensitivity of clone 6F11 (Kauffman O et al. Modern Pathology Hodgkin's lymphoma: immunohistochemical staining for Epstein-Barr virus-induced gene 3 11(4):357-363 (1998)) and Kaplan P A et al. American Journal of Clinical protein using NCL-EBI-3. Note cytoplasmic staining of infected cells. Paraffin section. Pathology 123: 276-280 (2005). NCL-ER-6F11/2 and NCL-L-ER-6F11/2 are more economic options for high volume users of clone 6F11. Refer to page 28 for the Bond ready-to-use format. Epstein-Barr virus (LMP-1)
Clone CS1/CS2/CS3/CS4 cocktail 1 mL, 0.1 mL lyophilized NCL-EBV-CS1-4 F P (Enzyme) Epstein-Barr virus (EBV) is one of the eight known human herpes viruses and belongs to the Gammaherpes viriniae, the same subfamily as human herpesvirus type 8 (HHV-8). Herpes viruses have large double strand DNA genomes and are complex viruses often encoding over 35 proteins including enzymes involved in nucleic acid metabolism, DNA synthesis and protein processing in addition to viral structural proteins. These viruses are capable of entering a latent phase where the host shows no visible signs of infection Human breast carcinoma: immunohistochemical staining for estrogen receptor using and levels of infectious agent become very low. During latency, viral gene NCL-L-ER-6F11. Note intense nuclear staining of tumor cells. Paraffin section. expression is restricted to only a few genes. Latent membrane protein (LMP- 1) is a 60 kD protein encoded by the BNLF1 gene of EBV. Product Specific Information NCL-EBV-CS1-4 is a cocktail of four monoclonal antibodies; clones CS1, CS2, CS3 and CS4.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. ER AND PR ANTIBODIES • Multiple formats – Novocastra liquid concentrate, Novocastra ready-to-use and Bond ready-to-use Novocastra liquid concentrate, –Novocastra formats Multiple • Validated clinical for use • labeled increased confidence for diagnostic IVD • cancer. breast of outcome therapy of and prognosis prediction as an management, aid inthe format are availableantibodies inIVD 16) (clone Receptor 6F11) and (clone Receptor Progesterone Estrogen Leica Microsystems’ CLEAR ARE RESULTS THE W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence ™ ready-to-use O E te applications Other lcrnmicroscopy Electron 107 /
Primary Antibodies Estrogen and Progesterone Receptor Antibodies (duo packs)
Clone 6F11 and Clone 1A6 2×1mLlyophilized NCL-ER/PGR-312d/1 F P (HIER) W 2 × 0.5 mL lyophilized NCL-ER/PGR-312d F P (HIER) W 2×1mLlyophilized NCL-ER/PGRd/1 F P (HIER) W 2 × 0.5 mL lyophilized NCL-ER/PGRd F P (HIER) W Product Specific Information Human tonsil: immunohistochemical staining for Ets-1 oncoprotein using NCL-ETS-1. For convenience, Leica Microsystems offer two antibodies in one pack. Note nuclear staining in a proportion of lymphocytes. Paraffin section. NCL-ER/PGR-312d/1 and NCL-ER/PGRd/1 are2x1mLunit alternatives to the 2 x 0.5 mL duo packs and are recommended as a more economic option for high volume users of these antibodies. Excitatory Amino Acid Transporter 2
Clone 1H8 Estrogen Receptor (beta) 1 mL lyophilized Excitatory Amino Acid Transporter 2 NCL-EAAT2 F P (HIER) Clone EMR02 Human excitatory amino acid transporters (EAATs) are members of a family of 1 mL lyophilized NCL-ER-beta P (HIER) W high affinity sodium-dependent transporter molecules that regulate neurotransmitter concentrations at the excitatory glutamatergic synapses of the mammalian central nervous system. It is reported that these proteins are Estrogen Receptor alpha (ER ) and beta (ER ) are the translated products thought to reduce extracellular glutamate concentration, thereby modulat-ing of separate genes located on different chromosomes. Although both synaptic signalling to replenish glutamate levels and prevent glutamate isoforms share a high degree of amino acid homology, the role of the induced excitotoxicity. A decrease in glutamate transporter activity has been conserved domains demonstrate specific functions. The A/B region, D Primary Antibodies associated with amyotrophic lateral sclerosis and excitotoxicity may be causal domain and F domains are notably distinct in sequence. ER is the highly or exacerbating in neurodegenerative diseases, including cerebral ischemia characterized estrogen receptor cloned originally from a human breast and epilepsy. EAAT1 is reported to be prominently expressed in the cerebellum, cancer cell line with ER more recently identified in rodents and now in frontal cortex, hippocampus and basal ganglia, is a potent antagonist and also humans. ER is reported to be expressed as multiple isoforms. ER , unlike appears to specifically block amino acid transport mediated by EAAT2. ER, is widely expressed being found in normal adult tissues of ovary, fallopian tube, lung, kidney, brain, heart, prostate and testis.
Ets-1 Oncoprotein
Clone 1G11 1 mL, 0.1 mL lyophilized NCL-ETS-1 F P (HIER) W The proto-oncogene c-Ets-1 is a transcription factor known to regulate expression of a number of genes involved in extracellular matrix remodelling. The processes of tumor invasion and metastasis are thought to Human brain, normal adult cerebellum: immunohistochemical staining for excitatory amino acid transporter 2 using NCL-EAAT2. Note intense membrane staining of glial cells. Paraffin depend on the increased proteolytic activity of the invading tumor cells that section. may involve matrix metalloproteinases, cathepsins B and D and plas- minogen activator in the metastatic cascade. Ets-1 interacts with the urokinase-type plasminogen activator gene enhancer and with the promoters of stromelysin-1 (MMP3) and collagenase-1 (MMP1) gene which may implicate it in this process. Ets-1 is reported to be absent from normal gastric epithelium, but is expressed in approximately 60 percent of gastric carcinomas and oral squamous cell carcinomas. The Ets-1 proto-oncogene is also preferentially expressed in lymphoid cells, where it is essential for the maintenance of the normal pool of resting T and B cells. Ets-1 expression level and distribution are differentially controlled in resting, activated and apoptotic lymphocytes.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. e loHmnvnWlern atr(atrVI-eae nie)o page on antigen) VIII-related (Factor Factor 123. Willebrand von Human also See PA0400 ready-to-use Bond mL 7 NCL-L-vWF liquid mL 1 NCL-vWF lyophilized mL 0.1 mL, 1 36B11 Clone Factor) Willebrand (von Antigen VIII-Related Factor section. Paraffin cells. malignant using of antigen staining EZH2 nuclear for Note staining NCL-L-EZH2. immunohistochemical lymphoma: cell B large Diffuse and buffer wash TBS-based in used when systems. diluent optimally stains NCL-L-EZH2 Information Specific Product as well lymphopoiesis. ensure as of regulation genes proteins the homeobox to PcG expressing contributing by regulation. development cycle axial embryonic in cell correct involved and complexes hematopoiesis repressing 2 patterning, gene Homolog Zeste multimeric of form (Enhancer EZH2 (Drosophila)) as such (PcG) proteins Polycomb-group NCL-L-EZH2 liquid mL 0.1 mL, 1 6A10 Clone (Drosophila)) 2 Homolog Zeste of (Enhancer EZH2 (HIER) P F HE)W (HIER) P (HIER) P F (HIER) P F atezm eeae ntebodcauaincsae ti Ca a is It cascade. coagulation blood the is the factor, in fibrin-stabilizing generated and fibrinoligase enzyme as last known also XIIIa Factor PA0449 ready-to-use Bond mL 7 NCL-FXIIIa lyophilized mL 0.1 mL, 1 E980.1 Clone XIIIa) Factor Coagulation (Blood XIIIa Factor C-AD oeitnemmrn n yolsi tiigo eeoigftlsi,internal skin, section. fetal Paraffin developing organs. of and staining components cytoplasmic using and protein membrane intense domain Note death NCL-FADD. Fas-associated for staining immunohistochemical fetus: Human testicular resistant of be to in be modulation apoptosis. known to Fas-mediated expressed to tumors reported the also unless carcinomas, be in is hepatocellular death FADD in to implicated apoptosis. downregulated cell cell reported is germ induce controlling been it by not has homeostasis where does It by testis but concentration. characterized mammalian cells high mechanism normal in death of present cell cytoplasm exists a the FADD fragmentation. is in DNA and pathway condensation cytoplasmic this and nuclear The of Fas cascade. the protease N- result to cysteine the a caspase-8 end activating unmasks recruit thereby to domains and it complex death allowing signalling FADD C-terminal of their domain through effector interaction terminal Fas The apoptosis. and with initiates FADD and interacts a Fas of which of is domain kD) Mort-1, death (23 intracellular as the protein known domain-containing also death (FADD), cytoplasmic Protein Domain Death Fas-Associated NCL-FADD lyophilized mL 1 64A6 Clone Protein Domain Death Fas-Associated format. ready-to-use Bond the for 28 page to Refer n srai,a nrae ubro atrXIapstv el nteupper described. the been in has cells lymphocytes, with positive associated XIIIa eczema closely factor dermis, of atopic In number eg increased phagocytes. dermatoses an inflammatory psoriasis, mononuclear and as to such common conditions features skin Langerhans benign some from specific distinct share a cells, which represent dendritic and skin cells dermal normal human marrow in in bone expression cells of positive population its XIIIa determine Factor to skin. examined epithelial inflamed been interstitial connectivehas dendrophages. dendritic and XIIIa collagen-associated positive positive collagenous Factor XIIIa stroma CD34 factor most scattered of and mammary populations like cells resident stroma, of contains mammary tissue proliferation and Normal development the elements. embryonic proliferation, through cell and growth with tissues animal associated in been widespread are have centers active thiol with glutaminases ufc oeue n ebae.TeeCa These cell stabilize membranes. to function and also its may molecules and XIIIa clot fibrin Factor surface the proteolysis. of to stabilization fibrin mechanical resistance the between in resulting crosslinks molecules gamma-glutamyl-epsilon-lysine intermolecular eedn rnguaiaeo rnaiaigezm hc forms which enzyme transamidating or transglutaminase dependent W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence (HIER) P F (HIER) P (HIER) P 2+ O E dpnettrans- -dependent te applications Other lcrnmicroscopy Electron 109 / 2+ -
Primary Antibodies Fas-Associated Phosphatase-1 Fascin
Clone AC21 Clone IM20 1 mL lyophilized NCL-FAP-1 P (HIER) 1 mL, 0.1 mL lyophilized NCL-FASCIN P (HIER) W Fas-associated phosphatase-1 (FAP-1) is a protein tyrosine phosphatase 7 mL Bond ready-to-use PA0420 P (HIER) that interacts with the cytosolic, negative regulatory domain of Fas and Human fascin is a 55 to 58 kD actin-bundling protein, whose actin binding inhibits Fas-mediated apoptosis. FAP-1 expression is reported to be ability is regulated by phosphorylation. In normal tissues the detection of expressed in normal renal tubules, skeletal muscle, myocardium, pituitary fascin is reported to be predominantly restricted to dendritic cells and in the gland, parathyroid gland, pancreatic islets, hepatocytes, testicular germ thymus has been observed only in medullary dendritic cells. In reactive nodes, cells, prostatic glands, neurons, endometrial glands, trophoblasts, bronchial interdigitating reticulum cells of T cell zones, cells in subcapsular areas, and epithelial cells and some types of gastrointestinal epithelial cells. Expression cells of the reticular network express fascin. Variable expression is seen in is highest in these tissues but variable expression of FAP-1 is reported in follicular dendritic cells and endothelial cells. Lymphoid cells, myeloid cells breast, stomach, colon and lung carcinomas as well as in several types of and plasma cells do not express fascin. However, in cases of Hodgkin's sarcoma. The expression of FAP-1 in normal tissues is reported to partly disease, including nodular sclerosis, mixed cellularity lymphocyte depletion overlap with Fas expression which may suggest that FAP-1 has an important and unclassified cases, most or all Reed Sternberg cells are reported to be role in the regulation of apoptosis. positive for fascin. Fascin expression may be induced by Epstein-Barr virus (EBV) infection of B cells with the possibility that viral induction of fascin in lymphoid or other cell types must also be considered in EBV-positive cases. Refer to page 29 for the Bond ready-to-use format.
Normal human thyroid: immunohistochemical staining for Fas-associated phosphatase-1 using
Primary Antibodies NCL-FAP-1. Note intense cytoplasmic staining of thyroid epithelial cells. Paraffin section.
Human Hodgkin's lymphoma: immunohistochemical staining for fascin using NCL-L-FASCIN. Note intense cytoplasmic and membrane staining of a proportion of Reed Sternberg cells. Fas (CD95) Paraffin section.
Clone GM30 1 mL lyophilized NCL-FAS-310 F P (HIER) Fas Ligand Fas is a 48 kD transmembrane glycoprotein. It is a member of the nerve growth factor receptor/tumor necrosis factor superfamily. This cell surface Clone 5D1 molecule mediates receptor-triggered apoptosis (programmed cell death). 1 mL lyophilized NCL-FAS-L P (HIER) W During embryonic and postembryonic development, many cells die by means of apoptosis. This plays a major role in determining morphological Fas ligand, a cell surface molecule belonging to the tumor necrosis factor and functional maturity in a variety of systems, including the formation of the family, binds to its receptor Fas, thus inducing apoptosis. Various cells neural network and clonal deletion of autoreactive T cells. Apoptosis is express Fas, whereas Fas ligand is reported to be expressed predominantly accompanied by condensation of the cytoplasm, loss of plasma membrane on activated T cells. Fas and Fas ligand are involved in the downregulation microvilli and extensive degradation of chromosomal DNA into oligomers of of immune reactions as well as T cell-mediated cytotoxicity. It is known that about 180 base pairs. The Fas antigen is reported to be expressed on the tumor necrosis factor (TNF) works as a cachectin and mediates septic surface of various cell types, including activated T and B lymphocytes and shock, so like TNF, Fas ligand may work as an agent that causes tissue T lymphoblastoid cell lines. damage. The Fas/Fas ligand system has been implicated both in maintaining immune privilege and also as a key regulator in spermatogenesis.
Human small intestine: immunohistochemical staining for Fas antigen (CD95) using NCL-FAS-310. Note membrane staining of absorptive epithelial cells. Paraffin section. Human prostate: immunohistochemical staining for Fas ligand using NCL-FAS-L. Note membrane and cytoplasmic staining of glandular epithelial cells. Paraffin section.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. C-I sseii o h elatcmn oano ua fibronectin. human of domain cases. some attachment in staining cell enhance may the pretreatment for Enzyme specific malignant is many NCL-FIB of stroma Information the Specific has Product collagen, in fibronectin expressed cells, Cellular widely macromolecules. bacteria, be other tumors. of to mRNA, of sequences. binding reported of variety repeat been the splicing a and multiple with and domains of heparin correlate numerous result domains of a These glycoprotein as a number exists A by producing integrins. isotypes cells the molecules, of fibronectin adhesion surface cellular of extracellular the of the to family a in bound disulfide-linked of are and members kD Fibronectins membranes matrix. 200 basement tissue in two connective found of are They composed subunits. glycoproteins are Fibronectins NCL-FIB lyophilized mL 0.1 mL, 1 568 Clone Fibronectin NCL-1G9. with immunoblotted FCV with infected cells A, Lane CRFK NCL-1G9. B, using Lane kD) markers. (62 protein weight capsid molecular (FCV) Calicivirus feline of detection blot: Western kD. 62 of protein capsid a these, of one 7.7kb detects NCL-1G9 of genome Information a Specific Product has FCV upper ulceration, polyarthritis. proteins. the several oral and encodes on including which infection symptoms, cats depressions tract of clinical pathogen cup-like of respiratory ubiquitous a variety of is unique a (FCV) Calicivirus of series producing Feline viruses virus. a the RNA of positive-stranded by surface of characterized family a morphology are Caliciviridae The NCL-1G9 lyophilized mL 0.5 1G9 Clone protein) (capsid Calicivirus Feline W FP iagi loapast eatre oeuefrremti arthritis- rheumatoid for molecule target a contagiosum. be humans. molluscum in to auto-antibodies and specific keratinizing appears human psoriasis also of parakeratosis, number Filaggrin as a in such their reported and disorders been variations species has to wide expression able exhibit then aberrant Filaggrins are IFs. the which keratin molecules At filaggrin with individual interact IFs. by to processed with linker proteolytically the interaction is of precursor of excision the incapable differentiation, terminal and of which, filaggrin, phosphorylated time granules of is keratohyalin as mouse, molecule epidermis in the precursor in A accumulates profilaggrin, epidermis. filament-associated terminally of intermediate mammalian (IFs) filaments the differentiating intermediate of keratin with class interact which important proteins an are Filaggrins NCL-FILAGGRIN lyophilized mL 1 15C10 Clone Filaggrin egtmres(mrhmLf cec) aeB R- el muolte ihNCL-FIL. with immunoblotted cells MRC-5 B, Rainbow Lane A, Science). Lane Life NCL-FIL. (Amersham using markers kD) (250 weight protein filamin of detection blot: Western filamin. rat and pig guinea chicken, rabbit, with cross-reacts NCL-FIL the at site Information binding Specific actin Product an polypeptide with identical end, two one at of other composed between other. the is link to It joined flexible each muscle. a chains in forming filaments protein actin crosslinking two a as functions Filamin NCL-FIL lyophilized mL 1 PM6/317 Clone Filamin section. Paraffin keratinocytes. differentiated terminally Note of NCL-FILAGGRIN. staining using cytoplasmic filaggrin intense for staining immunohistochemical skin: human Normal W P F etr blotting Western Paraffin Frozen C I HE)W (HIER) P F lwcytometry Flow Immunofluorescence (HIER) P O E te applications Other lcrnmicroscopy Electron TM molecular 111 /
Primary Antibodies Folate Receptor Alpha Galectin-1
Clone BN3.2 New! Clone 25C1 1 mL, 0.1 mL liquid NCL-L-FRalpha P (HIER) Reference Range 1 mL, 0.1 mL lyophilized NCL-GAL1 P (HIER) W Folate is a basic component of cell metabolism and DNA synthesis and Galectin-1 is a member of the beta-galactoside-binding family and is a repair. It is involved in essential one-carbon transfer reactions and is a pleiotropic dimeric protein of 14 kD participating in a variety of normal and vitamin required by both normal and tumor cells. Folate entry into cells is pathological processes, including cancer progression. Galectin-1 can affect facilitated via two different systems: the reduced folate carrier, which the proliferation of normal and malignant cells. Inhibition of cell growth is utilizes a bidirectional anion-exchange mechanism, and the folate receptor observed in a lactose-dependent manner as lower concentrations of the system. Folate receptor alpha is a membrane–bound member of the folate lectin stimulate cell proliferation. Galectin-1 may also be implicated in the receptor family, facilitating folate transport via a mechanism termed induction of apoptosis of activated T cells through the binding of exogenous potocytosis where the receptor is internalized and then recycled back to the galectin-1 to CD45 molecules present on the surface of lymphocytes. cell membrane. Staining patterns are both membrane and cytoplasmic due Galectin-1, reported to be present either at the surface of cancer cells or to this mechanism. Members of the folate receptor family share highly accumulated around these cells could act as an immunological shield to conserved sequences in the open reading frames, but differ in amino acids protect against a T cell immune response and provide an advantage for in the 5’ untranslated regions and as a consequence can differ in function survival. Galectin-1 is reported to be expressed by a variety of malignant and tissue expression. Folate receptor alpha expression is reported to be tumors including thyroid carcinoma. In colon carcinomas, the progressive highly restricted in normal tissues and only selectively overexpressed in a overexpression of galectin-1 has been reported to be demonstrated during limited number of epithelial malignancies. the evolution from normal to malignant cell type. Galectin-1 has not been detected in the cells of normal prostate, prostatic intra-epithelial neoplasia or prostatic carcinoma cells. However, galectin-1 is reported to be detectable in the stroma and associated fibroblasts of these tissues and is significantly increased in the tumor-associated stroma compared with non- neoplastic gland-associated stroma in a proportion of these. Three laminin binding proteins, galectin-1 together with galectin-3 and laminin receptor have been shown to effect similar qualitative and quantitative cell surface changes in cancer cells allowing them to cross basement membranes during metastatic spread. These changes in expression are reported in breast, colon, ovarian and uterine cancers. Primary Antibodies
Ovarian tumor: immunohistochemical staining for Folate Receptor Alpha using NCL-L-FRalpha. Note intense cytoplasmic staining. Paraffin section.
Folylpolyglutamate Synthetase
Clone AS2 New! 1 mL, 0.1 mL liquid NCL-L-FPGS P (HIER) Normal human prostate: immunohistochemical staining for Galectin-1 using NCL-GAL1. Folic acid is a water soluble vitamin, essential for normal cell growth and Note staining in the stroma and cytoplasmic staining of fibroblasts. Paraffin section. replication. Eukaryotes, however are unable to synthesize folates and therefore require an external source. Following uptake by the cell, folates are retained within the cell by polyglutamation, catalyzed by folylpolyglutamate synthetase (FPGS). Folates act as carriers of one carbon units, which are vital for the biosynthesis of purines, thymidylate and hence Galectin-3 DNA replication. Polyglutamation by FPGS increases binding of folate co- factors to enzymes of folate biosynthesis, prevents efflux of folate co-factors Clone 9C4 from the cell and allows the accumulation of folates required for glycine synthesis in the mitochondria. FPGS also plays an important role in the 1 mL, 0.1 mL lyophilized NCL-GAL3 P (HIER) W cellular retention of folate analogs/antifolates and is reported to play a role 7 mL Bond ready-to-use PA0238 P (HIER) New! in the selective cytotoxicity of such compounds used for the treatment of Galectin-3 is a member of the beta-galactosidase-binding lectin family. It is human cancers. involved in several biological events including binding to the basement membrane glycoprotein laminin. Cell surface galectin-3 may be involved in homotypical cell adhesion and is downregulated in colon cancer as the disease progresses. This downregulation has also been examined in breast carcinoma with a similar correlation of expression reported. Downregulation of galectin-3 could be one of the many events that enable cancer cells to interact with laminin to facilitate invasion and metastasis and may indicate activation of the invasive phenotype in various tumor types. Galectin-3 has been reported to be detected in anaplastic large cell lymphomas, whereas galectin-3 is not detected in Reed Sternberg cells or variants of Hodgkin's disease. Refer to page 29 for the Bond ready-to-use format.
Human adrenal medulla: immunohistochemical staining for folypolyglutamate synthesase (FPGS) using NCL-L-FPGS. Paraffin section.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. soitdpoen4 sn C-A4.Nt nes tiigo ern n apical and neurons of section. staining Paraffin intense dendrites. Note NCL-GAP43. growth using for 43 staining protein immunohistochemical associated cortex: cerebral adult normal brain, Human inhibit calmodulin, non-neuronal in bind filopodia can enhance and GAP43 its or release cells. although neurotransmitter unclear, kinase protein affect development and by is C, phosphorylated proteins be vivo during the kinase, associated phosphate is in phosphatidylinositol GAP43 growth either neurons. action the adult axons, beennormal of intact in has characterized in extending found It best higher axons for is injured are plasticity. expression of candidate synaptic regeneration GAP43 a that systems, and neuronal is growth neurons several It in axon terminals. that both reported presynaptic in certain involvement and neuronal of protein cones major a growth is (GAP43) 43 phosphoprotein associated Growth NCL-GAP43 lyophilized mL 1 1G7 Clone GAP43 section. Paraffin cells. epidermal malignant of staining membrane using intense gamma-catenin Note for NCL-G-CAT. staining immunohistochemical carcinoma: cell squamous Human follicular papillary, is of be majority expression the to in Gamma-catenin lost reported carcinomas. junctions. totally thyroid also or anaplastic cell and partially are be to 1 catenins to grade cell reported carcinomas, some at and those thyroid of 2 downregulated In behavior grade The aggressive both cells. the carcinoma. with of cancer link front a bladder E- invasive supporting and the further including of carcinomas, at beta-catenin carcinomas, found gamma-catenin, loss is of human or cadherin expression in of decrease decrease progression its variety combined neoplastic with where a associated strictly integrity in be in to role epithelial reported primary is in a of expression tumor plays expressed with E-cadherin maintenance be urothelium. associated to as the reported well be are as of to epithelium catenins types normal reported the many and been in E-cadherin detected has down- progression. been The and has forms. carcinomas catenins membrane-associated human and and cadherins soluble of with of protein in complex regulation cytoplasmic occurs a major that form a kD which is 82 cadherins plakoglobin, by or mediated Gamma-catenin is catenins. adhesion cell to Cell NCL-G-CAT lyophilized mL 1 11B6 Clone Gamma-Catenin HE)W (HIER) P F P ee opg 2frteBn ed-oueformat. ready-to-use Bond the for 22 page octapeptide. to Refer cholecystokinin cells. producing with gastrin-analogue or cross-reacts gastrin as labels well NCL-GASp antibody as gastrin-17 (II) The sulfated and gastrin-34. (I) non-sulfated with reacts NCL-GASp Information Specific cells Product immunoreactive glands. mucous the the hyperplasia of from cell clustering buds project by G sometimes characterized which secondary be and to primary gastrin-14, reported forms: are Both three gastrin-34. in and naturally occurs gastrin-17 hormone, polypeptide a Gastrin, PA0681 ready-to-use Bond mL 7 NCL-GASp lyophilized mL 0.5 Polyclonal Gastrin C--eii.Nt nes ula tiigo rlfrtn epatccls aafnsection. Paraffin cells. neoplastic proliferating of staining using nuclear Geminin intense Note for NCL-L-Geminin. staining immunohistochemical leukemia: lymphocytic chronic Human colon and carcinoma breast lymphoma, non- as cell skin such the cancers B of in carcinoma. upregulated epithelium lymphoma, be squamous to the reported Hodgkin’s of is germinal layers Geminin of be breast. basal tubules eg to and seminiferous the spermatocytes, cells that within reported colon, in testes, epithelial proposed is as the well and Geminin been as tonsil protein. lymphocytes has in suppressor centers proliferating It transition tumor and in mitosis. S cycle a to expressed cell during be G1 the the maximum may of in rest Geminin a expressed the through first reaching rising is the levels levels in but found expression cycle, not with cell is control phase, the Geminin the Cdt1. in of with involved phase interaction be G1 to the thought via acids replication amino DNA 209 of of protein a is Geminin NCL-L-Geminin liquid mL 1 EM6 Clone Geminin section. Paraffin mucosa. gastric the of staining NCL-GASp. cytoplasmic using intense gastrin Note for staining immunohistochemical stomach: human Normal W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence (HIER) P FP P New! O E te applications Other lcrnmicroscopy Electron 113 /
Primary Antibodies Giardia intestinalis
Clone 9D5.3.1 1 mL, 0.1 mL lyophilized NCL-GI P (HIER) Giardia intestinalis (formerly Giardia lamblia) is a flagellated protozoan, which infects humans via contaminated water supplies, causing illnesses ranging from acute severe bloody diarrhoea, through moderate enteritis, chronic diarrhoea with malabsorption, to asymptomatic excretion. The remarkable hardiness of the cyst form and the low numbers required to infect make the epidemiology uncertain, although water-based infections are the most common. Human pancreas: immunohistochemical staining for Glucagon using NCL-GLUCp. Note cytoplasmic staining in the endocrine cells of the islets. Paraffin section.
Glial Fibrillary Acidic Protein Glucocorticoid Receptor Clone GA5 1 mL, 0.1 mL lyophilized NCL-GFAP-GA5 FP Clone 4H2 7 mL Bond ready-to-use PA0026 P (HIER) 1 mL lyophilized NCL-GCR P (HIER) W Glial fibrillary acidic protein (GFAP) is an intermediate filament protein of The glucocorticoid receptor of molecular weight 90 kD has three main 52 kD reported to be expressed in glial cells eg astrocytes and ependymal functional regions; the N-terminal modulating region, the DNA binding cells. In the peripheral nervous system, GFAP has been reported to be region and the C-terminal steroid binding region. The glucocorticoid expressed in Schwann cells, enteric glial cells and satellite cells of human receptor is reported to be widely distributed and expressed in many cultured sensory ganglia and in neoplastic tissues GFAP has been reported to be cell lines eg CEM-C7. Glucocorticoid receptor is reported to be expressed in expressed in astrocytomas and ependymomas. neoplastic cells of chronic lymphocytic leukemia (CLL). Two isoforms of glucocorticoid receptor exist; alpha and beta, with the alpha form usually Product Specific Information the most abundant. The control of gene expression by glucocorticoids has
Primary Antibodies When using NCL-GFAP-GA5 the heat induced epitope retrieval (HIER) been widely studied as a model for transcriptional regulation. Glucocorticoid technique may improve staining in some cases. receptors are reported to induce or repress the expression of genes in response to glucocorticoids, mediating such processes as cell growth, Refer to page 30 for the Bond ready-to-use format. differentiation and apoptosis. Glucocorticoid receptors may also form a complex with heat shock protein 90 and in certain instances render the non- ligand bound receptor transcriptionally inactive. Product Specific Information Glucagon NCL-GCR is raised to the N-terminal modulating region. Polyclonal 0.5 mL lyophilized NCL-GLUCp FP Polyclonal 7 mL Bond ready-to-use PA0594 P (HIER) Glucagon expression has been reported in the endocrine cells of the pancreatic islets and also in the mucosa of small and large intestine. Pancreatic glucagon, a peptide of 29 amino acids, has biological activities including glycogenolysis, lipolysis, gluconeogenesis and ketogenesis. These effects are all antagonistic to insulin action and, therefore, lead to increased blood sugar levels. The majority of glucagonomas are reported to arise from the pancreas and produce pancreatic glucagon. These tumors are found Human tonsil: immunohistochemical staining for glucocorticoid receptor using NCL-GCR. chiefly in the main body or tail of the pancreas. Note nuclear staining in a wide distribution of cell types. Paraffin section. Refer to page 30 for the Bond ready-to-use format.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. C-Sm-3.Nt yolsi tiigo etl el.Prfi section. Paraffin cells. sertoli of staining using cytoplasmic mu Note S-transferase NCL-GSTmu-437. glutathione for staining immunohistochemical testis: Human from released epoxides of detoxification in of role expression is a GSTmu The leukemias. play smoke. myeloid cisplatin. cigarette to acute in drug known high the be also to to reported been to resistance has reported GSTmu are with GSTpi of associated levels cancer high in be carcinomas, breast Likewise, cell agents. some squamous chemotherapeutic in lung with human reported associated from are been GSTpi have GSTpi differ and and of to levels cells levels reported decreased High In showing are levels. levels. GSTalpha pi increased with differing and tissue at GSTalpha gastric skin of normal levels and the kidney bowel, cancers, breast, stomach, gastric pancreas, including liver, tissues, singly normal heart, be of to brain, variety reported evolutionary a are four in isoenzymes These multi-expressed into theta. or classified and pi been mu, carcinogens. have alpha, neutralizing classes; in isoenzymes role GST a carcino- play cytosolic initiate to The believed can are compounds GSTs reactive the by and and genesis as DNA such of endogenous components modification both cellular The of with DNA. react substrates. detoxification can the electrophilic which electrophiles in to exogenous involved glutathione are of enzymes conjugation These isoenzymes of the family catalyze multigene a which are (GSTs) S-transferases glutathione The (pi) S-Transferase NCL-GSTpi-438 Glutathione lyophilized mL 0.1 mL, 1 LW29 Clone NCL-GSTmu-437 (mu) S-Transferase Glutathione lyophilized mL 1 10H6 Clone NCL-GSTal-436 (alpha) S-Transferase Glutathione lyophilized mL 1 38H11 Clone Antibodies (GST) S-Transferase Glutathione FP P P e loC4b(Pb npg 79. page on (GPIb) CD42b also See NCL-CD42b lyophilized mL 1 MM2/174 Clone (CD42b) GPIb receptor. GnRH the of domain extracellular the for specific is NCL-GnRHR and Information luteal Specific Product These in in preparations. receptors reported membrane GnRH of pituitary. cell been those anterior ovarian to the characteristics has in binding similar as have GnRH well receptors as for of cells presence sites granulosa The receptors. binding specific as via hormone high-affinity hormone luteinizing stimulating secrete follicle to as pituitary well anterior the stimulates reproduc- of (GnRH) the gonadotrophs hormone the regulates the Gonadotropin-releasing sexes. It in both systems. in mediator endocrine cycle and tive key neural the the of is integration hormone decapeptide Gonadotropin-releasing NCL-GnRHR lyophilized mL 1 A9E4 Clone Receptor Hormone Gonadotropin-Releasing e loC6 GII)o ae82. page on (GPIIIa) CD61 also See PA0308 ready-to-use Bond mL 7 NCL-CD61-308 lyophilized mL 0.1 mL, 1 2f2 Clone (CD61) GPIIIa 79. page on (GPIIb/IIIa) CD41 also See NCL-CD41 lyophilized mL 1 M148 Clone (CD41) GPIIb/IIIa W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence F (HIER) P F (HIER) P (HIER) P (HIER) P F O E te applications Other lcrnmicroscopy Electron 115 /
Primary Antibodies Granulysin Gross Cystic Disease Fluid Protein-15
Clone RJT48 Clone 23A3 1 mL liquid NCL-L-Granulysin P (HIER) 1 mL, 0.1 mL lyophilized NCL-GCDFP15 P (HIER) Granulysin is a member of the Saposin-like protein (SAPLIP) family. It is a 1 mL liquid NCL-L-GCDFP15 P (HIER) lytic protein made up of two fragments, 15 kD and 9 kD, reported to be 7 mL ready-to-use RTU-GCDFP15 P (HIER) expressed selectively by NK cells and activated T cells. Granulysin protein is 7 mL Bond ready-to-use PA0350 P (HIER) highly homologous to a porcine antimicrobial and antitumor protein called NK lysine and to amebapores, polypeptides used by amoebae to kill Gross cystic disease of the breast is a benign premenopausal disorder in bacterial prey. Granulysin disrupts artificial liposomes and cell membranes, which cysts are a predominant pathological lesion. These cysts appear to damages mitochondria and activates caspase 9 to induce apoptosis in be formed from excessive apocrine cystic secretions. This fluid is composed nucleated cells. of several glycoproteins including a unique 15 kD monomer protein, GCDFP15. It has been reported that cytosolic analysis of normal tissue from all major organs has demonstrated GCDFP15 in apocrine epithelia, lacrimal, ceruminous and Moll's glands and in numerous serous cells of the submandibular, tracheal, bronchial, sublingual and minor salivary glands. Cytosol from breast carcinoma lesions are reported to contain GCDFP15 at a wide range of concentrations. The concentration is reported to be highest in more differentiated carcinomas and GCDFP15 shows only a few positive individual epithelial cells within lobules and small ducts in normal breast. Expression has also been reported in fibroadenomas within areas of apocrine metaplasia. GCDFP15 and prostate specific antigen are reported to be co-expressed in androgen receptor-positive breast tumors. Refer to page 30 for the Bond ready-to-use format. Human NK cell lymphoma: immunohistochemical staining for granulysin using NCL-L- Granulysin. Note intense granular cytoplasmic staining on a proportion of NK cells. Paraffin section. Primary Antibodies Granzyme B
Clone 11F1 1 mL, 0.1 mL lyophilized NCL-GRAN-B P (HIER) 1 mL liquid NCL-L-GRAN-B P (HIER) 7 mL ready-to-use RTU-GRAN-B Human breast, Paget's disease: immunohistochemical staining for gross cystic disease fluid P (HIER) protein (15 kD) using RTU-GCDFP15. Note variable cytoplasmic staining of tumor cells. Paraffin 7 mL Bond ready-to-use PA0291 P (HIER) section. Granzymes are neutral serine proteases which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTL) and in natural killer (NK) cells. These CTL and NK cells are heavily involved in the elimination of neoplastic and virally infected cells. Secretory granules containing perforin and H-CAM (CD44) granzymes are instrumental in undertaking cytolytic activity. Granzyme B is understood to enter a target cell through a perforin pore-formed channel to induce DNA fragmentation and apoptosis. Granzyme B has also been Clone DF1485 described in neoplastic CTL and NK cells. 1 mL, 0.1 mL lyophilized NCL-CD44-2 F P (HIER) Refer to page 30 for the Bond ready-to-use format. See also CD44 (H-CAM) on page 79.
Hodgkin's disease: immunohistochemical staining for granzyme B using NCL-L-GRAN-B. Note granular cytoplasmic staining in a number of Reed Sternberg cells. Paraffin section.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. C-S7 sratv ihHp0adha hc ont 0(s7)i man, in (Hsc70) 70 rat. cognate and shock mouse heat and Hsp70 with reactive is NCL-HSP70 of most Information activities, Specific normal Product their heat to cytoplasm. from return the to to recovery denatured returns begin Hsp70 During the nucleoli the turnover. the solubilizing as their by and facilitate or shock proteins to refold ribosomal proteins helping by unfolded ribosomal either the bound perhaps its of nucleolus, and cytoplasm, the some the in in proteins state, in protecting free exists its state, Hsp70 between biosynthesis. processing ischemicequilibrium cytokine in antigen in in cell role in role the regulatory role possible protective a Heat potential a as proteins. well a have as and shock to or infection reported heat heat inflammation, is of to disease, (Hsp70) exposure induction 70 as the protein such with shock stress, associated to organisms is or chemicals, cells of response The NCL-HSP70 lyophilized mL 1 8B11 Clone 70 Protein Shock Heat section. Paraffin cells. tumor of staining NCL-HSP27. cytoplasmic using intense Hsp27 Note for staining immunohistochemical carcinoma: breast Human of expression that reported pathway. common is receptor estrogen most it functional tissue, a the of breast is evidence as In heavy kD) taken man. is to (27 Hsp27 in exposure Hsp27 found shock, infection. Hsp heat viral small sublethal and including hormones (Hsps) stress, proteins metals, shock to heat response of variety in a express eukaryotes and Prokaryotes NCL-HSP27 lyophilized mL 1 2B4 Clone 27 Protein Shock Heat (HIER) P FPW irgi n srcts nrae xrsinrpre during reported during role expression important an have Increased may development. Hsp105 mouse astrocytes. that with suggests brain embryogenesis and the oligodendrocytes, in heat neurons, markedly occurs all after nearly microglia Hsp105 nucleus are in the of expression cytoplasmic both to expression and translocate nuclear in abundant and may localized most but specifically heart The cells is shock. and Hsp105 germ testis, of liver the cytoplasm In the spleen, shock. expressed heat the glands, moderately after be in increased adrenal condit- to found stressed reported the are and be isoforms non-stressed in to Hsp105 under rodents, reported nucleoli In the are ions. in isoforms of not a regulator but Hsp105 both a beta cytoplasm as as The and and acts proteins cells. alpha Hsp105 of mammalian isoforms; aggregation family. thermal two protein prevent Hsp105/Hsp110 as to chaperone the exists to (Hsp105) belong 105 which protein shock Heat NCL-HSP105 lyophilized mL 1 58F12 Clone 105 Protein Shock Heat Hsp90. sex of localization MHC with nuclear cancer, with correlated correlate breast to be reported In small is carcinomas. expression to of I endometrial cells, class reported cells in epithelial been status endothelial receptor in has and steroid expressed cells each expression nerve be Hsp90 subcomplexes, cells, to vessels. folding giant reported these discrete cells, been in mononuclear function and has several that Hsp90 receptors co-chaperones forms pathways. of hormone groups steroid Hsp90 distinct containing as folding non-physiological kinases. the such including with protein proteins associated stress, is of signal-transducing (Hsp90) 90 kinds of organisms protein all various shock nearly Heat by in temperatures. induced proteins conserved are highly and are proteins shock Heat NCL-HSP90 lyophilized mL 1 JPB24 Clone 90 Protein Shock Heat C-S15 oeitnectpamcadncersann fPrij el n neuronal and cells Purkinje section. of Paraffin staining processes. nuclear using and 105 cytoplasmic protein intense shock Note heat NCL-HSP105. for staining immunohistochemical cerebellum: brain, Human W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence HE)W (HIER) P HE)W (HIER) P O E te applications Other lcrnmicroscopy Electron 117 /
Primary Antibodies Helicobacter pylori Hepatitis B virus Antibodies
Clone ULC3R New! Clone LF161 1 mL, 0.1 mL liquid NCL-L-Hpylori P (Enzyme) 1 mL, 0.1 mL lyophilized Hepatitis B virus (core antigen) NCL-HBcAg-506 P Polyclonal 1 mL lyophilized NCL-HPp P (Enzyme) Clone 1044/341 Helicobacter pylori is a motile, helix–shaped Gram–negative, 1 mL lyophilized Hepatitis B virus (surface antigen) microaerophilic, bacterial pathogen which is capable of converting from a NCL-HBsAg-2 F P (Enzyme) spiral form to a coccoid form to favor its survival. Almost 50 percent of the world’s population, approaching 100 percent in some countries, are Hepatitis B virus is one of an expanding list of hepatitis viruses. The infected. There are numerous strains of Helicobacter pylori which can be complete infective virion is a 42nm particle (Dane particle). The infective grouped into two broad families, type I and type II, based on their expression virion consists of a core of double stranded DNA, a specific DNA of the hopQ allele. Type I and type II strains are reported to express VacA polymerase and structural proteins surrounded by an outer envelope, (vacuolating toxin) responsible for vacuolation of gastric epithelial cells and Hepatitis B surface antigen (HBsAg). The nucleocapsid contains two induction of apoptosis. Type I strains are reported to express CagA protein serologically distinct antigens; core antigen and ‘e' antigen. Core antigen is which is associated with deregulation of intercellular signalling pathways localized predominantly within the nucleus of infected hepatocytes, and initiation of pathogenesis (virulent strains) and are closely related to whereas ‘e' antigen is found in the cytoplasm of infected hepatocytes. A gastric diseases such as peptic ulceration, gastric ulceration, chronic significant proportion of carriers infected with the Hepatitis B virus may gastritis, mucosa–associated lymphoid tissue (MALT) lymphoma and develop persistent infection, chronic hepatitis of various types, cirrhosis and intestine type gastric adenocarcinomas. Type II strains are reported not to possible primary hepatocellular carcinoma. express CagA proteins. HopE is a 31 kD porin protein which is part of a Product Specific Information family of 32 outer membrane proteins present in Helicobacter pylori NCL-HBcAg-506 recognizes core antigen which is localized predominantly bacteria. HopE is highly conserved in Helicobacter pylori strains, but not within the nucleus. NCL-HBsAg-2 reacts with surface antigen. among other strains of the Helicobacter genus. Product Specific Information Clone ULC3R, unlike polyclonal antibodies to Helicobacter pylori, does not cross-react with Campylobacter jejuni (a gastric bacterium which causes
Primary Antibodies infective diarrhoea). Clone ULC3R also exhibits more defined staining of H. pylori bacteria than NCL-HPp. The antibody clone ULC3R, will be useful to identify and differentiate patients that need antibiotic eradication of the bacterium from those patients who are at a higher risk of developing clinical disease related to H. pylori infection.
Human liver, hepatitis B positive: immunohistochemical staining for hepatitis B core antigen using NCL-HBcAg-506. Note intense nuclear staining of infected hepatocytes. Paraffin section.
Hepatitis C virus (NS3)
Clone MMM33 Human stomach infected with H. pylori: immunohistochemical staining for H. pylori using 1 mL, 0.1 mL lyophilized NCL-HCV-NS3 F P (HIER) NCL-L-Hpylori. Paraffin section. Hepatitis C virus (HCV) is the leading cause of blood-borne and community acquired non-A, non-B hepatitis. HCV infection has been estimated to affect about 3 percent of the population worldwide. Higher prevalence occurs in high-risk groups, which include individuals with a history of intravenous drug abuse and those multiply transfused before the introduction of mass screening of donated blood for viral antibodies. The virus persists in approximately 80 percent of those infected. Twenty percent of individuals with chronic infection progress to cirrhosis after an average of 20 years. Hepatocellular carcinoma is a significant risk in these, occurring in around 3 percent annually. Virus antigen has been reported in the cytoplasm of hepatocytes of infected individuals by immunohistochemistry although the sensitivity of detection of antigen has varied from study to study. Product Specific Information NCL-HCV-NS3 is a monoclonal antibody raised against a recombinant NS3 protein.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. nie sn C-S.Nt rnlrctpamcsann napooto fhepatocytes. of proportion a in section. staining Paraffin cytoplasmic granular specific Note hepatocyte NCL-HSA. for using staining antigen immunohistochemical positive: B hepatitis liver, Human staining. cytoplasmic and granular adult distinct a both produce in to hepatocytes present normal antigen fetal uncharacterized an recognizes NCL-HSA Information hepatocyte Specific in specific Product the expressed of are detection hepatocytes. especially fetal antigen the malignant carcinoembryonic and that hepatoblastoma, normal or reported fetoprotein some- is can of alpha childhood It antigens, of difficult. distinction tumors the cell be round of The times small tumor other primary from children. type, common embryonal most in the be liver to reported is Hepatoblastoma NCL-HSA lyophilized mL 0.1 mL, 1 OCH1E5 Clone Antigen Specific Hepatocyte 92. page on Receptor) Factor Growth (Hepatocyte c-MET also See NCL-cMET lyophilized mL 0.1 mL, 1 8F11 Clone (c-MET) Receptor Factor Growth Hepatocyte HCV-infected of section. staining Paraffin cytoplasmic non- hepatocytes. Note (HCV) NCL-HCV-NS3. virus using C 3 Hepatitis protein for structural staining immunohistochemical liver: human infected Acutely P (HIER) P F L . Llyophilized mL 0.1 mL, 1 liquid mL 1 domain) (external NCL-CBE-356 HER-2 lyophilized mL 0.1 mL, 1 10A7 Clone TA9145 System IHC Bond HER2 Oracle RTU-CB11 Tests domain) 60 (internal HER-2 ready-to-use mL 7 NCL-L-CB11 domain) (internal HER-2 liquid mL 1 domain) (internal NCL-CB11 HER-2 lyophilized mL 0.1 mL, 1 CB11 Clone NCL-c-erbB-2-316 domain) (internal HER-2 lyophilized mL 1 5A2 Clone Antibodies Oncoprotein) (c-erbB-2 HER-2 C-B-5.Nt nes ebaesann ftmrcls aafnsection. Paraffin cells. tumor of staining membrane using intense oncoprotein Note c-erbB-2 NCL-CBE-356. for staining immunohistochemical carcinoma: breast Human 11. page see System IHC Bond HER2 Oracle the on information obtain more epitope To recommended. For is induced cases. fixative some Carnoy's heat tissue, in the frozen staining on of staining enhance on optimal may use pretreatment technique the no (HIER) but with retrieval NCL-CBE-356, tissue effective oncoprotein. paraffin-embedded is of NCL-CB11 fixed, c-erbB-2 domain oncoprotein. external the c-erbB-2 the detect the of all RTU-CBE-356 and domain RTU-CB11 NCL-CBE1 NCL-L-CBE-356, internal and the NCL-PC11 detect NCL-L-CB11, NCL-CB11, NCL-c-erbB-2-316, breast, including Information tissues, Specific Product adult cell and of fetal variety human wide ovary. normal and a of stomach in range present a transitional surface as in is well types cell oncoprotein as of c-erbB-2 adenocarcinomas other family carcinomas. detectable and be cell large breast to of a reported proportion of is a epidermal oncoprotein in member c-erbB-2 the a receptors. to is factor structure growth and in receptor closely-related factor is growth oncoprotein c-erbB-2 The NCL-PC11 domain) (internal HER-2 lyophilized mL 0.2 Polyclonal NCL-CBE1 ln CBE1 Clone RTU-CBE-356 domain) (external HER-2 ready-to-use mL 7 W P F etr blotting Western Paraffin Frozen E- etra domain) (external HER-2 FPC (HIER) P F FPC PW FP Reference Range C I E- etra domain) (external HER-2 lwcytometry Flow Immunofluorescence NCL-L-CBE-356 O E te applications Other lcrnmicroscopy Electron PW FP FP 119 / P
Primary Antibodies Heregulin HGM-45M1 (Human Gastric Mucin)
Clone P137 Clone 45M1 1 mL lyophilized NCL-HEREG P (HIER) 1 mL, 0.1 mL lyophilized NCL-HGM-45M1 F P (HIER) Heregulins or neuregulins are a family of proteins known to interact with and See also Human Gastric Mucin (HGM-45M1) on page 121. activate the tyrosine kinase receptor c-erbB-2 in association with c-erbB-3 or c-erbB-4. Heregulin is reported to induce in vitro chemoinvasion and chemotaxis of breast cancer cells as well as growth in an anchorage dependent and independent manner. HLA Class II (DR) Antigen Product Specific Information NCL-HEREG is raised to the alpha form of the heregulin protein. Clone LN-3 1 mL, 0.1 mL lyophilized NCL-LN3 FP HLA-DR is an MHC Class II antigen that maps to chromosome 6. It is a heterodimer composed of 2 non-covalently associated glycoproteins of Herpes simplex virus Antibodies about 35 kD (alpha, heavy) and 27 kD (beta, light). Both chains are comprised of two Ig-like domains and have transmembrane sequences and short Clone 20.7.1 cytoplasmic tails. It is reported to be expressed mainly on antigen- 1 mL lyophilized Herpes simplex virus (type 1) presenting cells (monocytes/macrophages and dendritic cells), B cells and some activated T cells. Expression has also been reported on thymic NCL-HSV-1 P (Enzyme) I epithelial cells. 12.3.4, Clone 1.1.1 1 mL lyophilized Herpes simplex virus (type 2) NCL-HSV-2 I Infection with Herpes simplex virus (HSV) is extremely common and pathogenesis can vary depending on a variety of factors. These include age, immune status of the individual, the antigenic type of infecting virus (HSV Primary Antibodies type 1 or 2) and the site of infection. Primary infections with HSV are generally asymptomatic but they tend to be more severe than recurrent productive disease. Product Specific Information NCL-HSV-1 is HSV type 1 specific and does not cross-react with tissue Human anaplastic lymphoma: immunohistochemical staining for HLA class II antigen using culture grown HSV type 2 strains. NCL-HSV-2 is HSV type 2 specific and does NCL-LN3. Note membrane staining of large cells. Paraffin section. not cross-react with tissue culture grown strains of HSV type 1.
HMB45 (Melanoma Marker)
Clone HMB45 1 mL lyophilized NCL-HMB45 F P (Enzyme) Reference Range The HMB45 antigen has also been identified in retinal pigment epithelium (RPE) but is reported to be reactive only with the transient prenatal and infantile RPE. No reaction is reported to be observed with intradermal nevi and normal adult melanocytes and non-melanocytic cells. Tumor cells of epithelial, lymphoid, glial and mesenchymal origin are reported to be Herpes simplex virus type 1 (HSV1) infected HEp-2 cells: indirect immunofluorescence for negative. This clone is well described in the literature. It is indicated to label HSV1 using NCL-HSV-1. Note intense staining of HSV1 infected HEp-2 cells only. Acetone-fixed cells. an intracytoplasmic antigen in the majority of melanomas and other tumors demonstrating melanoma/melanocytic differentiation. The clone is also reported to react with junctional and blue nevus cells. (Bacchi CE et al., A Review. Applied Immunohistochemistry. 4:73-85 (1996)). HGH (Human Growth Hormone) Polyclonal 0.25 mL lyophilized NCL-HGH FP 7 mL Bond ready-to-use PA0704 P See also Human Growth Hormone (HGH) on page 121.
Human metastatic melanoma: immunohistochemical staining for melanoma cells using NCL-HMB45. Note cytoplasmic staining of malignant cells. Paraffin section
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. ee opg 1frteBn ed-oueformat. ready-to-use Bond the for stain 31 page therefore, to chorionic Refer may, human and pituitary. hormone the of in luteinising a cells beta-chain basophil shows with NCL-HCGp isolated cross-reaction trophoblasts. placental the slight with reacts to and raised gonadotrophin C- was intracellular the the NCL-HCGp that in believed role Information is Specific a It Product plays end. subunit COOH heterodimer. HCG-beta the its of the an behavior at contains of acids it region amino that in terminal in unique 29 is hormones of beta-subunit glycoprotein hCG extension beta-containing The luteinising respectively. of kD, of family 17 molecular the subunit and have kD chains the 14 beta of to hormone-specific weights the The identical and hormone. chain stimulating is and alpha follicle common alpha hCG and hormone subunits, of stimulating two thyroid subunit the hormone, of alpha after of composed maintains luteum The is days turn corpus beta. hCG in the 12 which pregnancy endometrium. progesterone to secretory of produce to binds the to trimester stimulated which 10 first is hCG, which the beginning of ovary in production placenta fetus the the the requires of of Maintenance cells conception. produced hormone trophoblastic glycoprotein a by is (hCG) gonadotrophin chorionic Human PA0014 ready-to-use Bond mL 7 NCL-HCGp lyophilized mL 1 Polyclonal (beta) Gonadotrophin Chorionic Human Paraffin syncytiotrophoblasts. of section. staining cytoplasmic Note gonadotrophin NCL-HCG-alpha. chorionic using human alpha for staining immunohistochemical placenta: human Normal subunits. beta and alpha free its breast positive in ERa of as hCGa. half non-seminomatous express approximately cancers and well that is suggest seminomatous mRNA studies as in Independent hCGa detected cancers peripheral gland. be the pituitary to testicular in reported the and Additionally, disease. is in women trophoblastic hCG with pregnant TSH patients placenta normal of and normal cells in mononuclear in FSH blood detected hCG be LH, of to of part reported as part expressed and as kD chain is 14 and alpha hCGa secreted of respectively. common weights four molecular kD, The the have 17 TSH. chains of beta and subunit hormone-specific breast FSH the alpha in been and LH, common gene hCG, now the responsive hormones, has encodes (ERa) glycoprotein It alpha gene cells. receptor (hCGa) estrogen cancer alpha an gonadotrophin as identified chorionic human The NCL-HCG-alpha lyophilized mL 1 4E12 Clone (alpha) Gonadotrophin Chorionic Human (Enzyme) P F (HIER) P F (HIER) P nacspoensnhss osre abhdae n ssu fat up uses and carbohydrates hormone conserves growth general, stores. synthesis, In muscle. metabolic protein and direct tissue exerts enhances adipose GH act growth liver, growth. which the cause stimulates to on somatomedins, GH tissue effects of soft kD. and production 22 bone liver's on of directly the weight promoting molecular pituitary by a anterior indirectly the has of GH cells metabolism. Human somatotropic organic or gland. in acidophilic by important synthesized also is is It and for growth responsible hormone body primary overall the regulating is somatotropin, (GH), hormone Growth PA0704 ready-to-use Bond mL 7 NCL-HGH lyophilized mL 0.25 Polyclonal (HGH) Hormone Growth Human upon stable is but proteolysis trypsin following lost oxidation. periodate partially epitope, is this destroys which completely NCL-MUC-1-CORE. 2-mercaptoethanol) antibody, (using the to reduction function Thiol of similar core a peptide fulfilling the mucin, in gastric located epitope mucin the recognizes NCL-HGM-45M1 Muc-1, Information several include Specific Product mucins” with “gastric associated The glycoproteins. Muc-6 tract. is epithelium and gastric gastrointestinal Muc-5AC turn of normal surface the in the on found of mucin which is mucin as earliest glycosylation Gastric Human the identified diseases. mucin with been associated in are have antigens steps these antigens of associated expression The cancer antigens. the of Many NCL-HGM-45M1 lyophilized mL 0.1 mL, 1 45M1 Clone (HGM-45M1) Mucin Gastric Human H8i eotdt eepesdi atezn ag immunoblastic large zone MCD, mantle In in MCD. expressed and Castleman's be KS in to intranodal cells. cells B between reported endothelial link is and the HHV8 explain begins, is may KS which disease proliferations, intranodal cell spindle early subcapsular where in its HHV8 of to lymphadenopathies. situ multicentric localization angioimmunoblastic in related The in expressed with in and be be complex (MCD) to a disease may reported also Castleman's forming been which of has the HHV8 capable and product activity. is is oncogenic LNA gene which PCR 73. (LNA) susceptibility orf situ antigen gene retinoblastoma viral nuclear the in latent of of in a product immunohistochemistry, agent demonstrated encodes been etiological HHV8 by has hybridization. proposed HHV8 that tissues the reported is is KS It (HHV8), (KS). sarcoma 8 Kaposi's type herpesvirus Human NCL-HHV8-LNA lyophilized mL 0.1 mL, 1 13B10 Clone antigen) nuclear (latent 8) (type Herpesvirus Human format. ready-to-use Bond the for 31 page to Refer W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence FP P HE)W (HIER) P (HIER) P F O E te applications Other lcrnmicroscopy Electron 121 /
Primary Antibodies Human Securin
Clone DCS-280.2 1 mL lyophilized NCL-SECURIN P (HIER) Human securin (hsecurin), also known as pituitary tumor-transforming gene- 1 (PTTG) product, is required for chromosomal stability in human cells. Abnormalities of chromosome number are reported to be amongst the most common genetic aberrations in cancer. The mechanisms for regulating mitotic chromosome transmission in mammalian cells are, therefore, of great interest. Human cells without an hsecurin gene lose chromosomes at Human Kaposi's sarcoma: immunohistochemical staining for HHV8 latent nuclear antigen a high rate. These losses have been linked to abnormal anaphases during using NCL-HHV8-LNA. Note nuclear staining in a proportion of infected tumor cells. Paraffin which cells undergo repeated unsuccessful attempts to segregate their section. chromosomes. Therefore, human securin is essential for the maintenance of euploidy. The expression of hsecurin is reported to correlate with cell proliferation in a cell cycle-dependent manner in both normal tissues and in several tumor types. hsecurin specifically binds to Ku, the regulatory subunit of the DNA-dependent protein kinase. Ku and hsecurin associate both in Human Neutrophil Defensins (1/2/3) vitro and in vivo. DNA double-strand breaks prevent Ku/hsecurin association showing that genome damaging events can result in the induction of Clone D21 pathways that activate DNA repair mechanisms and halt cell cycle 1 mL lyophilized NCL-DEFENSIN P (HIER) progression. It has also been proposed that hsecurin connects DNA- damage response pathways with sister chromatid separation delaying Defensins are antimicrobial agents which together with serprocidins, mitosis while DNA repair occurs. lysozyme, bacterecins, protegrins and indolicidin have been isolated from neutrophil and macrophage granules. Defensins are synthesized as 93 to 96 amino acid pre-propeptides. In fully differentiated phagocytes, virtually all of the cellular defensin exists as processed mature peptide. Neutrophil defensins are stored in azurophil granules which discharge their contents into microbe-containing phagosomes through the process of phagosome/ granule fusion. Paneth cells of the small intestine are also reported to Primary Antibodies secrete defensins, as well as lysozyme into the crypt lumen which may limit local microbial proliferation and colonization. These peptides may also exert chemotactic and immunomodulating effects in host defence and inflammation. The three principle human neutrophil defensin peptides, HNP 1, 2 and 3, are reported to be unique to neutrophils and account for 99 percent of the defensin content in these cells. Activation of neutrophils Human tonsil: immunohistochemical staining for human securin using NCL-SECURIN. leads to a rapid release of HNP which may also be measured in plasma and Note nuclear staining of proliferating cells. Paraffin section, nickel DAB. other body fluids in infection and inflammation.
Human Spasmolytic Polypeptide
Clone GE16C 1 mL lyophilized NCL-HSP P (HIER) Human spasmolytic polypeptide (HSP) is a member of the trefoil peptide family which is reported to be expressed in discrete regions of the body, most notably the gastrointestinal tract. In the stomach, HSP is reported to be localized to foveolar and surface epithelium, pyloric glands and mucous Human tonsil: immunohistochemical staining for human neutrophil defensins using NCL- neck cells. DEFENSIN. Note intense granular cytoplasmic and extracellular staining of neutrophils. Paraffin section.
Normal human stomach: immunohistochemical staining of human spasmolytic polypeptide using NCL-HSP. Note cytoplasmic staining of cardiac glands. Paraffin section.
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Primary Antibodies Immunoglobulin D Immunoglobulin M
Clone DRN1C Clone 8H6 1 mL, 0.1 mL liquid NCL-L-IgD P (HIER) Reference Range 1 mL, 0.1 mL liquid immunoglobulin M NCL-L-IgM P (HIER) W Clone DRN1C was developed to produce reduced background staining that is Reference Range associated with polyclonal antibodies on paraffin sections. Clone 8H6 was developed to produce reduced background staining that is IgD, together with IgM, are the major immunoglobulins expressed on the associated with polyclonal antibodies on paraffin sections. surface of B cells where it seems they may operate as mutually interacting IgM, together with IgD, is the major immunoglobulin expressed on the antigen receptors for the control of lymphocyte activation and suppression. surface of B cells and normally constitutes about 10 per cent of serum The greater susceptibility of IgD to proteolysis in combination with antigen immunoglobulin. IgM antibody is prominent in early immune responses to could well be implicated in such a function. most antigens and predominates in certain antibody responses such as Product Specific Information ‘natural’ blood group antibodies. The use of PBS-based diluents may result in increased background staining. Clone DRN1C was developed to produce reduced background staining that is associated with polyclonal antibodies on paraffin sections.
Human tonsil: immunohistochemical staining for immunoglobulin M using NCL-L-IgM. Note staining of follicular dendritic cells, mantle zone B cells and intense staining of plasma cells. Paraffin section.
Human tonsil: immunohistochemical staining for Immunoglobulin D using NCL-L-IgD. Note Primary Antibodies intense membrane staining of B cells. Paraffin section. Immunoglobulin Antibodies Immunoglobulin G Polyclonal 1 mL lyophilized immunoglobulin A Clone RWP49 New! NCL-IgAp P (Enzyme) W 1 mL, 0.1 mL liquid NCL-L-IgG P (HIER) Polyclonal Clone RWP49 was developed to produce reduced background staining that is 1 mL lyophilized immunoglobulin D associated with polyclonal antibodies on paraffin sections. NCL-IgDp P (Enzyme) The human immunoglobulins consist of two identical heavy chains (~50 kD) and two identical light chains, which are linked together by disulphide bonds. The Polyclonal light chains can be either kappa or lambda. The five immunoglobulins IgA, IgD, 1 mL lyophilized immunoglobulin G IgE, IgG and IgM differ in their heavy chains, and IgA and IgM differ as they can occur in polymeric forms. The heavy chain of IgG is named the gamma-chain. In NCL-IgGp P (Enzyme) W humans, IgG consists of four sub classes that differ only marginally in their amino acid composition. Antibodies to IgG have been reported to be useful in Polyclonal the identification of plasma cells, lymphoid cells containing IgG and classifying 1 mL lyophilized immunoglobulin M B cell derived neoplasms. The normal B cell population is polyclonal, NCL-IgMp P (Enzyme) W expressing a range of different immunoglobulins. In contrast, the majority of B cell neoplasms are characterized by the proliferation of monoclonal cells The basic structure of immunoglobulin (Ig) molecules is a tetramer of two expressing one type of light chain, whereas more than one type of heavy chain light chains and two heavy chains linked by disulfide bonds. There are two can be expressed by the same cell. IgG positive neoplasms include hairy cell types of light chains, kappa and lambda, each composed of a constant leukemia, splenic lymphoma and follicular lymphoma. domain (CL) and a variable domain (VL). There are five types of heavy chains: alpha, delta, epsilon, gamma and mu, all consisting of a variable domain (VH) and three (in alpha, delta and gamma) or four (in epsilon and mu) constant domains (CH1 to CH4). Product Specific Information NCL-IgAp, NCL-IgDp, NCL-IgGp and NCL-IgMp have each been solid-phase absorbed to remove cross-reactivity.
Human tonsil: immunohistochemical staining for Immunoglobulin G using NCL-L-IgG. Paraffin section.
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Primary Antibodies Involucrin Ki67 Antigen
Clone SY5 Clone MM1 1 mL, 0.1 mL lyophilized NCL-INV F P (Enzyme) 1 mL, 0.1 mL lyophilized NCL-Ki67-MM1 F P (HIER) Involucrin is a precursor (120 kD) of the epidermal cornified envelope which 1 mL liquid NCL-L-Ki67-MM1 F P (HIER) Reference Range becomes cross-linked during envelope assembly. Involucrin is expressed in 7 mL ready-to-use RTU-Ki67-MM1 F P (HIER) a range of stratified squamous epithelia, including the cornea which lacks a 7 mL Bond ready-to-use PA0118 P (HIER) distinct cornified layer and is expressed when differentiation is terminated. In normal dermis, involucrin is expressed in the upper cornified layer. New! However, in pathological conditions, involucrin expression is altered eg in Clone K2 psoriasis and other benign epidermal hyperplasias, where involucrin 7 mL Bond ready-to-use PA0230 P (HIER) expression is found closer to the basal layer. Expression of involucrin is abnormal in squamous cell carcinomas and premalignant lesions. Polyclonal Product Specific Information 0.2 mL lyophilized NCL-Ki67p F P (HIER) When using NCL-INV, enzyme pretreatment may enhance staining in some The Ki-67 antigen is a human nuclear protein, which is expressed in all cases. active parts of the cell cycle (G1, S, G2 and mitosis), but absent in resting cells (G0). In contrast to many other cell cycle-associated proteins, the Ki67 antigen is consistently absent in quiescent cells and is not detectable during DNA repair processes. Thus, the presence of Ki67 antigen is strictly associated with the cell cycle and confined to the nucleus, suggesting an Kappa Light Chain important role of this structure in the maintenance and/or regulation of the cell division cycle. Clone CH15 New! Refer to page 32 for the Bond ready-to-use format. 1 mL, 0.1 mL liquid NCL-L-KAP-581 P (HIER) Reference Range Clone kp-53 1 mL lyophilized NCL-KAP FPW Kip2 (p57 Protein) Primary Antibodies Clone L1C1 Clone 25B2 1 mL, 0.1 mL lyophilized NCL-KAP-L1C1 F P (Enzyme) 1 mL, 0.1 mL lyophilized NCL-p57 P (HIER) Clone L1C1 is the main choice of end users for use on paraffin sections. Cyclin dependent kinases are positive regulators of cell proliferation. p57 Immunoglobulins are polypeptides and comprise five major classes; protein acts as a tumor suppressor to counter this. It is closely-related to immunoglobulin G (IgG), IgA, IgM, IgD and IgE. Each immunoglobulin other CDKIs such as p21 protein (CIP1) and p27 protein (Kip1) as they share a consists of two identical heavy (H) chains and two identical light (L) chains. common structural N-terminal domain for binding to CDK/cyclin complexes These are also subdivided into sub classes eg IgG1. There are two classes and inhibiting their kinase activity. Human p57 protein is found on of light chain; kappa and lambda. The ratio of kappa chains and light chains chromosome 11p15.5, a region which is reported to be a common site for varies between Ig classes and sub classes, but is also species specific. In loss of heterozygosity in certain sarcomas, Wilms’ tumors and tumors humans, approximately 60 percent of light chains are kappa. However, in associated with the Beckwith-Wiedemann syndrome. There is increasing any particular immunoglobulin molecule the light chain will be either kappa interest in p57 as a marker in Gestational disease. Gestational trophoblastic or lambda. B cells contain either kappa or lambda mRNA. disease refers to a spectrum of proliferative disorders of the placental trophoblast, with a wide range of histologic appearances and clinical behaviors. Recent developments in changes in the criteria for histologic diagnosis of these lesions due to earlier clinical diagnosis have been reviewed Hui P et al., Advantages in Anatomical Pathology. 12(3): 116-125 (2005) and the ability to make more accurate diagnoses due to the introduction of newer antibodies such as p57 is discussed.
Tonsil: immunohistochemical staining for Kappa Light Chain using NCL-L-KAP-581. Note cytoplasmic staining of plasma cells. Paraffin section.
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Primary Antibodies LFA-2 (CD2) L-selectin (CD62L)
Clone AB75 Clone 9H6 1 mL, 0.1 mL lyophilized NCL-CD2-271 P (HIER) 1 mL lyophilized NCL-CD62L-489 P (HIER) 1 mL liquid NCL-L-CD2-271 P (HIER) The CD62L antigen is also known as Leu-8, TQ1, LAM1, MEL-14 antigen, 7 mL ready-to-use RTU-CD2-271 P (HIER) lymph node homing antigen and L-selectin. It mediates the tethering and rolling of leukocytes on endothelial surfaces and also contributes to the See also CD2 (LFA-2) on page 70. recruitment of leukocytes from the blood to areas of inflammation. CD62L antigen is also important for the homing of naive lymphocytes to peripheral lymph nodes and Peyer's patches and can also mediate neutrophil to neutrophil interactions via the recognition of CD162 antigen. CD62L antigen is reported to be expressed on the surface of mantle zone B lymphocytes in Linker for Activation of T Cells different lymphoid sites but is absent on germinal center B cells. It is also expressed on a proportion of T cells in peripheral lymph nodes, mucosal Clone 3.8 lymphoid sites and spleen. Non-lymphocytic staining has been reported on 1 mL liquid NCL-L-LAT F P (HIER) Langerhans cells, follicular dendritic cells in tonsil, neutrophils, monocytes and on macrophages in the thymus. Ligands for CD62L are expressed not 7 mL ready-to-use RTU-LAT F P (HIER) only in specific vascular endothelium in lymph nodes and Peyer's patches Stimulation of the T cell antigen receptor (TCR) results in the activation of but also in extravascular tissues such as brain white matter, the choroid several protein tyrosine kinases (PTKs) associated with the TCR. These plexus and in kidney distal tubuli. activated PTKs phosphorylated tyrosine residues on multiple protein substrates. This phosphorylation results in the activation of enzymes such as phospholipase C gamma or creates sites of binding for proteins involved in the activation cascade. Linker for activation of T cells (LAT) is an integral membrane protein (36 to 38 kD) which plays an important role in linking engagement of the TCR to the biochemical events of T cell activation. LAT is a substrate of activated ZAP-70 and Syk PTKs. It binds following tyrosine phosphorylation, Grb2, PLC-gamma1 and other critical signalling molecules recruiting them to the plasma membrane. This has the effect of enhancing
Primary Antibodies the phosphorylation of tyrosine residues required for enzymatic activation and promoting the formation of protein complexes. LAT mRNA is found in NK cells and mast cells. LAT protein has been reported to be detected in thymus and peripheral lymphoid tissues such as T cell areas in lymph nodes and Human tonsil: immunohistochemical staining for CD62L antigen (L-selectin) using spleen. In the small intestine, intra-epithelial T cells also express LAT, and in NCL-CD62L-489. Note membrane staining of lymphocytes. Paraffin section. bone marrow, LAT is expressed by T lymphocytes in interstitial spaces and also by platelets and megakaryocytes. LAT is reported not to be expressed in B cells, macrophages, plasma cells, monocytes, epithelial histiocytes and dendritic cells. Lung Resistance-Related Protein (110 kD)
Clone 9D6 1 mL lyophilized NCL-LRRP P (HIER) W Multidrug-resistant cancer cell lines are reported to frequently overexpress the 100 kD lung resistance protein (LRP) also known as lung resistance- related protein (LRRP). The overexpression of LRRP in acute myeloid leukemias, multiple and ovarian carcinomas has been reported. LRRP functions as a major vault transporter protein where vaults are multi-subunit structures which may be involved in nucleocytoplasmic transport. LRRP is overexpressed in P-glycoprotein negative multidrug-resistant tumor cell Human tonsil: immunohistochemical staining for LAT protein using NCL-L-LAT. Note intense lines of different histogenetic origins and show an ATP-dependent drug membrane staining of T lymphocytes. Paraffin section. accumulation effect. LRRP is also reported to be expressed in normal tissues, with expression being highest in epithelial cells with secretory and excretory functions such as bronchial cells and intestinal epithelial cells. LMP-1 (Epstein-Barr virus) CS1, CS2, CS3, Clone CS4 1 mL, 0.1 mL lyophilized NCL-EBV-CS1-4 F P (Enzyme) See also Epstein-Barr virus (LMP-1) on page 106.
Normal human testes: immunohistochemical staining for lung resistance-related protein (110 kD) using NCL-LRRP. Note cytoplasmic staining of cells within the seminiferous tubules. Paraffin section.
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Primary Antibodies MAGE-1 Mast Cell Chymase
Clone 6C1 Clone CC1 1 mL lyophilized NCL-MAGE-1 P (HIER) 1 mL lyophilized NCL-MCC P (HIER) The human MAGE gene products are recognised by major Chymase is an enzyme found in human mast cells and acts as a mediator of histocompatability complex-restricted cytotoxic T lymphocytes. MAGE-1, inflammation and matrix remodelling. Mast cells are present in most human also known as tumor rejection antigen, is a target for immunotherapy in tissues and have themselves been implicated in angiogenesis, inflammation patients with hepatocellular carcinoma (HCC). MAGE-1 is reported to be and fibrosis. Mast cells are not a single cell type but represent a highly expressed in about 60 per cent of HCC cases. Other studies utilising reverse heterogenous population. Subpopulations differ in their responsiveness to transcriptase-PCR and southern blot hybridisation techniques have reported various secretagogues, their susceptibility to pharmacological control by MAGE genes to be expressed in malignant tumors and pre-cancerous anti-allergic drugs and also the extent to which they may be histologically lesions but not in benign tumors. stained using basic dyes. Mast cells may contain both chymase and tryptase in their secretory granules (MCTC) or tryptase only (MCT) without chymase. The MCTC population normally predominates at connective tissue sites and is also most abundant in skin, heart, gastrointestinal submucosa Maspin and respiratory submucosa tissues. The MCT cells are most numerous in mucosal tissues. Chymase, one of the major secretory products of MCTC Clone EAW24 cells, may alter cytokine bioavailability by activating the interleukin-1b (IL- 1b) precursor, degrading IL-4 and liberating membrane-bound stem cell 1 mL, 0.1 mL lyophilized NCL-MASPIN P (HIER) factor. It could also participate in matrix remodelling by activating Maspin, or mammary-specific serpin, is a tumor suppressor protein of 42 kD procollagenase and control blood flow by generating angiotensin II. In that belongs to the serine proteinase inhibitor (serpin) family. It is reported to be animal models, chymase has also been shown to increase microvascular expressed in normal breast and prostatic epithelial cells but is downregulated permeability and promote the accumulation of inflammatory cells. in carcinomas derived from these cell types. The expression of maspin is controlled at the transcriptional level by a combination of elements including Ets, AP-1 and p53. The tumor suppressor activity of maspin may depend on its ability to inhibit angiogenesis. In breast myoepithelial cells, maspin is predominantly a soluble cytoplasmic protein which associates with secretory vesicles and is present at the cell surface. The loss of maspin in breast tumors
Primary Antibodies is reported to be a progressive process and expression decreases with increasing malignancy of primary tumors and is absent from lymph node and distant metastases. In rats, maspin mRNA has been detected in mammary gland, vagina, bladder, thymus, small intestine, ventral prostate, seminal vesicles and thyroid, but is absent from heart, lung, liver, brain and kidney.
Human tonsil: immunohistochemical staining for mast cell chymase using NCL-MCC. Note intense cytoplasmic staining of mast cells. Paraffin section.
Mast Cell Tryptase
Clone 10D11 1 mL, 0.1 mL lyophilized NCL-MCTRYP-428 P 7 mL Bond ready-to-use PA0019 P Human prostate: immunohistochemical staining for maspin using NCL-MASPIN. Note cytoplasmic and nuclear staining of normal glandular basal cells. Paraffin section. Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. A substantial quantity of tryptase is reported to be found in mast cells of skin and lung and suggests this enzyme plays a major role in mast cell mediated events. In vitro studies indicate tryptase can cleave C3 to form C3a anaphylatoxin, inactivate fibrinogen as a coaguable substrate for thrombin and activate latent collagenase. Models of allergenic disease in the skin, nose and lung have each indicated elevated tryptase levels. Human mast cell tryptase has been reported to be implicated as a mediator of inflammation. Mast cell degranulation in the gut causes mucus secretion, mucosal edema, increased gut permeability and may be responsible for some of the symptoms and signs of inflammatory bowel disease. Refer to page 33 for the Bond ready-to-use format.
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Primary Antibodies MDM2 Protein Melanoma Marker (CD63)
Clone 1B10 Clone NKI/C3 1 mL, 0.1 mL lyophilized NCL-MDM2 F P (HIER) 1 mL, 0.1 mL lyophilized NCL-CD63 FP The human phosphoprotein homolog of the murine double minute 2 (MDM2) See also CD63 (Melanoma Marker) on page 82. gene, with a molecular weight of 90 kD (p90), forms a complex with both mutant and wild type p53 protein. The MDM2 gene product interacts with p53 protein inhibiting p53-mediated transactivation. Overexpression of MDM2 overcomes wild type p53 mediated suppression of transformed cell growth. MDM2 amplification is reported to be observed in some soft tissue Melanoma Marker (HMB45) sarcomas, osteosarcomas and high grade malignant gliomas. Product Specific Information Clone HMB45 NCL-MDM2 reacts with the human homolog of MDM2. 1 mL, 0.1mL lyophilized NCL-HMB45 F P (Enzyme) Reference Range See also HMB45 (Melanoma Marker) on page 120.
Merosin Laminin Alpha 2 Chain
Clone Mer3/22B2 1 mL lyophilized NCL-MEROSIN F The dystrophin-glycoprotein complex is localized to the muscle membrane. Several members of this complex are reported to be implicated in muscular Human breast carcinoma: immunohistochemical staining for MDM2 protein using NCL-MDM2. dystrophy. Dystrophin expression is altered in Duchenne and Becker Note nuclear staining of tumor cells. Paraffin sectiion. muscular dystrophy and four types of limb girdle muscular dystrophy are caused by mutations in the genes for alpha, beta, gamma and delta-
Primary Antibodies sarcoglycan. An extracellular member of this complex is alpha-dystroglycan and linked to this, in the extracellular matrix, is laminin. The muscle specific Melan A form of laminin, merosin, is composed of three chains: alpha 2, beta 1 and gamma 1. Mutations in the chromosome 6 encoded gene for the laminin Clone A103 alpha 2 chain of merosin are responsible for a form of congenital muscular dystrophy (CMD). Merosin negative CMD is characterized by a severe 1 mL, 0.1 mL lyophilized NCL-MelanA F P (HIER) W clinical phenotype and is associated with white matter changes on brain 1 mL liquid NCL-L-MelanA F P (HIER) W Reference Range imaging. 7 mL ready-to-use RTU-MelanA F P (HIER) Product Specific Information 7 mL Bond ready-to-use PA0233 P (HIER) NCL-MEROSIN reacts with the 300 kD fragment of merosin (Sewry et al. Melan A, a product of the MART-1 gene, is a melanocyte differentiation Muscle and Nerve Supplement. 7, S109: (1998)) labeling with an antibody to marker recognized by autologous cytotoxic T lymphocytes. Other beta-spectrin to monitor membrane integrity, is an essential immuno- melanoma-associated markers recognized by autologous cytotoxic T cells histochemical control. are reported to include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1 and GAGE-1. The analysis of these different molecules and their expression AB in individual melanomas may be of help in the study of their particular molecular roles in melanocyte differentiation and tumorigenesis. Refer to page 33 for the Bond ready-to-use format.
Human skeletal muscle: immunohistochemical staining for merosin using NCL-MEROSIN. Note membrane staining of normal muscle fibers (A) and absence of staining of muscle fibers in an individual with chromosome 6-linked congenital muscular dystrophy (B). Frozen sections. Photographs supplied courtesy of Dr Louise V B Anderson.
Human melanoma: immunohistochemical staining for melan A using NCL-MelanA. Note cytoplasmic staining of melanoma cells. Paraffin section.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. ln 4A srpre ob eciewt h IFMisoform. MITF-M the with reactive be to reported is 34CA5 Clone Information Specific Product N-terminus the at structurally differ other MITF-M. MITF-H, The from melanocytes. and malignant MITF-C and MITF-A, normal for in isoforms, expressed necessary be The to melanocytes. reported MITF-M, enzymes factor is of transcription viability the microphthalmia of postnatal isoform critical encoding and melanocyte-specific is development MITF genes nuclear embryonic TRP-2. the a and of for TRP-1 a play tyrosinase, to regulation include thought product, These the melanogenesis. is type, gene in basic-helix-loop-helix role the (MITF) of factor factor transcription transcription Microphthalmia NCL-L-MITF liquid mL 0.1 mL, 1 NCL-MITF lyophilized mL 1 34CA5 Clone (MITF) Factor Transcription Microphthalmia section. Paraffin cells. tumor of staining membrane NCL-MESO. intense using Note mesothelin for staining immunohistochemical mesothelioma: Human which from format. ready-to-use cells Bond the mesothelial for 33 normal page to the Refer of derived. in are function occasional cystadenocarcinomas abundant ovarian The Mesothelin and in adhesion. be mesotheliomas tube. cellular malignant in to and fallopian role a reported and have kidney may is tonsil it the but trachea, unclear interest. is the in mesothelin generated of expressed has cells which abundantly epithelial form is N-terminal membrane-bound but kD. GPI-linked factor, Mesothelin an 40 potentiating the of megakaryocyte into the is form to membrane-bound it processed identical GPI-linked is the enzymatically form and secreted kD The is 30 of which a form as secreted precursor synthesized is kD It epithelial carcinomas. mesotheliomas, cell 69 cells, squamous mesothelial some and of of cancers surface ovarian glycoprotein the on (GPI) present kD glycosyl-phosphatidylinositol-linked 40 a is Mesothelin PA0373 ready-to-use Bond mL 7 RTU-MESO ready-to-use mL 7 NCL-L-MESO liquid mL 1 NCL-MESO lyophilized mL 1 5B2 Clone Mesothelin (HIER) P F (HIER) P F (HIER) P F (HIER) P F (HIER) P F (HIER) P rti NCL-MCM7 7 Protein Maintenance Minichromosome lyophilized mL 1 DCS-141.1 Clone NCL-MCM5 5 Protein Maintenance Minichromosome lyophilized mL 1 CRCT5.1 Clone NCL-MCM3 3 Protein Maintenance Minichromosome lyophilized mL 1 JCC07 Clone NCL-MCM2 2 Maintenance Protein Minichromosome lyophilized mL 0.1 mL, 1 CRCT2.1 Clone Antibodies Protein Maintenance Minichromosome section. Paraffin cells. melanoma of staining nuclear Note NCL-L-MITF. transcription using microphthalmia factor for staining immunohistochemical melanoma: malignant Human C-C2 C-C3 C-C5adNLMM r pcfcfor specific are respectively. 7, and NCL-MCM7 5 3, and 2, proteins chromosomal maintenance NCL-MCM5 minichromosome causing NCL-MCM3, protein NCL-MCM2, C- MCM7 the Information HPVs. Specific oncogenic human to Product of proteins bind E6 the expressing p53- to cells human have of in reported to abnormalities was seems region also E6 -18), but terminal functions. and p53, transforming (HPV-16 protein human suppressor independent cancer the tumor stoichiometric cervical of the E6 with in cell degrades Oncoprotein associated in complexes. participate present MCM (HPV), actively of papillomavirus molecules not part the as of are regulation proportion cycle a MCM3 proteins only and MCM7 and MCM the amounts MCM5 than and abundant Therefore, MCM2 less phase, much G0 proteins. progress be the In to cells cycle. reported normal cell are the as proteins of phase manner G1/S variable into MCM single-stranded G0 a helicase of from in Levels to complex. and increase protein generally MCM activity annealed an proteins are with ATPase oligonucleotides associated which are Both DNA, displaces domain circular mammals. central which to their activity, proteins yeast in MCM from the motifs of conserved Each ATPase replication. DNA-dependent DNA play eukaryotic have to in reported part been essential have an proteins (MCM) maintenance Minichromosome W P F etr blotting Western Paraffin Frozen HE)W (HIER) P W (HIER) P (HIER) P (HIER) P C I lwcytometry Flow Immunofluorescence O E te applications Other lcrnmicroscopy Electron 133 /
Primary Antibodies Mismatch Repair Protein (MLH1)
Clone ES05 1 mL, 0.1 mL liquid NCL-L-MLH1 P (HIER) Reference Range 7 mL Bond ready-to-use PA0610 P (HIER) New! MLH1, a mismatch repair protein involved in maintaining the integrity of genetic information, alongside MSH2, MSH6 and PMS2. During DNA replication, strand misalignment can occur resulting in alterations to microsatellite repeats, often referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been linked to human Human gastric carcinoma: immunohistochemical staining for minichromosome maintenance carcinogenesis. Mutations in the MLH1 gene have been reported to be protein 3 using NCL-MCM3. Note intense nuclear staining of proliferating tumor cells. found in tumors with MSI, such as some forms of colon cancer eg Paraffin section. Hereditary nonpolyposis colon cancer (HNPCC), a subset of sporadic carcinomas and breast cancer. Loss of expression of MLH1 has also been reported in acute lymphoblastic leukemia, endometrial carcinoma, gastric carcinoma and ovarian carcinoma. Minichromosome Maintenance Protein 6 Refer to page 33 for the Bond ready-to-use format.
Clone KAT82 1 mL, 0.1 mL liquid NCL-L-MCM6 P (HIER) W Minichromosome maintenance protein 6 (MCM6) is one of the six members of the MCM family, involved in the initiation of DNA replication. The binding of MCM proteins appears to make chromatin competent for replication, as MCM-free chromatin is unable to replicate. MCM6 is detectable in nucleosols or bound to nuclear chromatin during the G1, S and G2 phases of the cell cycle and bound to chromatin in the cytoplasm during mitosis. MCM6 is reported to be undetectable during the G0 phase. Due to its role as a replication licensing factor, MCM6 is thought to represent a marker of Primary Antibodies proliferating cells, with a similar expression pattern to Ki67 during the cell Human small intestine: immunohistochemical staining for MLH1 protein using NCL-L-MLH1. cycle (G1, S, G2 and M). However, reports suggest that MCM6 is expressed Note gradient of staining through the maturing and differentiated epithelial cells of the villi and during the early G1 phase, when Ki67 is undetectable, therefore suggesting also in a proportion of the stromal cells. Paraffin section. that a subset of proliferating cells in early G1 phase which are undetectable with Ki67 antibodies may be detectable with an MCM6 antibody. Mismatch Repair Protein (MSH2)
Clone 25D12 1 mL lyophilized NCL-MSH2 P (HIER) Reference Range 7 mL Bond ready-to-use PA0048 P (HIER) New! Human mismatch repair protein 2 (MSH2) is involved in the initial recognition of mismatched nucleotides during the post replication mismatch repair process. Therefore, the loss of MSH2 function leads to the accumulation of replication errors, which in turn may be responsible for the multiple Human mantle cell lymphoma: immunohistochemical staining for Minichromosome mutations required for multistage carcinogenesis. Mutations in mismatch Maintenance Protein 6 (MCM6) using NCL-L-MCM6. Note staining of proliferating cells. repair genes have been linked to hereditary nonpolyposis colon cancer and Paraffin section. to sporadic cancers which exhibit microsatellite instability. MSH2 is reported to be expressed in the nuclei of cells from a variety of tissues including thyroid, heart, smooth muscle and the germinal centers of lymphoid follicles. In ileum and colon, MSH2 expression has been reported in the crypts, the cells of which are undergoing rapid renewal. They are responsible for the continuous production of differentiated cells which migrate over 2 to 4 days before being sloughed into the lumen. Refer to page 34 for the Bond ready-to-use format.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. oeceryascae ihavraino NC nw sTurcot as known HNPCC of variation with but a tumors (HNPCC) with of Cancer of associated syndrome. Colon proportion dominantly proportion clearly Non-Polyposis the substantial of significant more Hereditary cases but with disorder a associated small inherited is for PMS2 a instability. and for microsatellite cancers account defects colorectal hMLH1 gene identifying analysis. in PMS2 mutation helpful for suitability be their can identify tumors and in carriers mutation expression repair a PMS2 that of forms proteins loss division. other The cell protein for of preparation binding PMS2 cell presence during the the arising The coordinates errors in DNA activated complex gene. then this is mutL ATP; which which of protein bacterial PMS1, MLH1 the kDa and the with 96 MLH3 heterodimer of a MLH1, gene is to homologs PMS2 PMS2 related 7. The are closely chromosome protein. protein on (MMR) clusters repair repair in mismatch found mismatch are protein DNA members PMS1 a family as is known also 2, (PMS2), homologue 2 increased segregation Postmeiotic NCL-L-PMS2 liquid mL 0.1 mL, 1 M0R4G Clone (PMS2) Protein Repair Mismatch cells. tumor of proportion a of section. staining Paraffin nuclear intense 6 Note Protein NCL-L-MSH6. Repair using Mismatch (MSH6) for staining immunohistochemical carcinoma: colonic Human format. ready-to-use Bond the for 34 staining. page background to increased Refer in result in may diluents PBS-based use of use to The marker Information useful Specific HNPCC. Product a as such mutations, be tumors gene MMR colon to contain identify may to that reported screening instability been microsatellite with microsatellite in conjunction has with lost carcinomas MSH6 be colonic colonic instability. can and carcinomas normal expression and endometrial lymphocytes. sometimes in carcinomas some gastric in However, in cells expressed found carcinomas. is of also MSH6 endometrial that is nucleus shown also Expression the that have crypts. Studies in reported in expressed especially have epithelium, strongly studies is Immunohistochemical DNA MSH MSH6 disrupted. other with cause is along expression, proteins, can MSH6 where Nonpolyposis (HNPCC) and Hereditary Cancer in Colorectal particularly has mutations cancer, instability colorectal in Microsatellite cause described unstable. been become can to sequences (MMR) systems microsatellite in repair Defects mismatch repair MSH2. DNA with in mismatch heterodimerized involved when is pathways, which recombination protein and kD 160 a is MSH6 PA0597 ready-to-use Bond mL 7 NCL-L-MSH6 liquid mL 0.1 mL, 1 PU29 Clone (MSH6) Protein Repair Mismatch New! Ezm)W (Enzyme) P (HIER) P (HIER) P New! Reference Range ratcrioa,sget hti ih aearl sasprso of suppressor a as and role biliary a pancreatic, have prostatic, might metastasis. gastric, gene, it or of tumorigenesis that MKK4 cancer proportion suggests colorectal the a carcinomas, and of in breast testicle and mutation breast, lines lung, and cell pancreatic deletion human in The of nucleus reported and epithelia. cytoplasm the gastric in in expression and normal pancreas, lower liver, with kidney, brain placenta, many and heart, in muscle expressed skeletal be including to tissues reported growth human been cell has forin mRNA result MKK4 signals usually kinases differentiation. extracellular which and of stresses protein proin- environmental transduction or the mitogen-activated and factors in growth activates involved stresses are MKK4 which cellular activates (MAPKs) kinase. and to phosphorylates p38/HOG also response MKK3, the like in MKK4, cytokines. c-Jun (JNK) flammatory the MAP activates kinases the and of phosphorylates member N-terminal directly a which is family (MKK4) kinase 4 kinase kinase kinase protein Mitogen-activated NCL-MKK4 lyophilized mL 1 7A6 Clone 4 Kinase Kinase Protein Mitogen-Activated NCL-L-PMS2. using section. PMS2 Paraffin for staining immunohistochemical Carcinoma: Colonic Human sn C-K4 oectpamcadncersann fsemtgnai h seminiferous the in section. spermatogonia Paraffin of tubules. staining nuclear 4 and kinase cytoplasmic kinase Note NCL-MKK4. protein using mitogen-activated for staining immunohistochemical testis: Human W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence (HIER) P O E te applications Other lcrnmicroscopy Electron 135 /
Primary Antibodies Motility-Related Protein-1 (CD9) Multiple Myeloma Oncogene 1 (MUM-1)
Clone 72F6 Clone EAU32 New! 1 mL lyophilized NCL-CD9 F P (HIER) 1 mL, 0.1 mL liquid NCL-L-MUM1 P (HIER) See also CD9 (Motility-Related Protein-1) on page 72. 7 mL Bond ready-to-use PA0129 P (HIER) The MUM-1 (multiple myeloma oncogene 1) gene was originally identified because of it’s involvement in the t(6:14) translocation observed in multiple myeloma, which causes the juxtaposition of the MUM-1 gene to the Ig heavy chain locus. MUM-1 is expressed in late plasma cell directed stages of Muc Glycoprotein Antibodies B cell differentiation and in activated T cells, suggesting that MUM-1 may serve as a marker for lympho-hemopoetic neoplasms derived from these Clone Ma552 cells. The morphologic spectrum of MUM-1 expressing cells has been found 1 mL lyophilized muc-1 core glycoprotein to range from that of a centrocyte to that of a plasmablast/plasma cell. NCL-MUC-1-CORE F P (HIER) Consequently the histogenic value of MUM-1 may be to provide a marker to aid in the identification of the transition from BCL-6 positive (germinal center B cells) to CD138 positive (immunoblasts and plasma cells). MUM-1 Clone Ma695 expression occurs in a wide range of lymphoid neoplasms including a 1 mL lyophilized muc-1 glycoprotein proportion of diffuse B cell lymphomas but not myeloid or extra-hemopoietic NCL-MUC-1 F P (HIER) neoplasms. MUM-1 is consistently expressed in myeloma cells, Reed Sternberg cells in classic Hodgkin Disease, and activated and neoplastic Clone Ccp58 T cells. 1 mL, 0.1 mL lyophilized muc-2 glycoprotein Refer to page 35 for the Bond ready-to-use format. NCL-MUC-2 F P (HIER) Clone CLH2 1 mL, 0.1 mL lyophilized muc-5AC glycoprotein NCL-MUC-5AC P (HIER) Primary Antibodies Clone CLH5 1 mL, 0.1 mL lyophilized muc-6 glycoprotein NCL-MUC-6 P (HIER) Mucins are heavily glycosylated proteins which constitute the major components of mucus covering the surface of epithelial tissues. Nine Human diffuse large B cell lymphoma: immunohistochemical staining for multiple myeloma distinct epithelial mucin genes (Muc-1, 2, 3, 4, 5AC, 5B, 6, 7 and 8) have been oncogene 1 (MUM-1) using NCL-L-MUM1. Paraffin section. identified. Various immunohistochemical and in situ hybridization studies have reported that these mucins are differentially expressed in epithelia with cell-type specificity. The normal gastric mucosa shows cell-type specific expression of Muc-1, Muc-5AC and Muc-6 glycoproteins. Muc-1 and Muc-5AC are found in superficial epithelium and Muc-6 glycoprotein in Multi-Cytokeratin the deep glands. Muc-1 and Muc-5AC glycoproteins are reported to be expressed in many epithelia but Muc-6 glycoprotein is mainly expressed in Clone AE1, Clone AE3 gastric mucosa. In addition, Muc-2 glycoprotein is not expressed in normal 1 mL lyophilized NCL-AE1/AE3 F P (HIER) gastric mucosa. In gastric cancer, alterations in mucin polypeptide 1 mL liquid NCL-L-AE1/AE3 F P (HIER) expression have been reported, including the loss of expression of Muc-5AC glycoprotein, increased mucin heterogeneity, glycosylation changes and the 7 mL ready-to-use RTU-AE1/AE3 F P (HIER) expression of simple mucin-type carbohydrates. 7 mL Bond ready-to-use PA0909 P (Enzyme) Keratins are a family of water insoluble proteins of 40 to 70 kD. These proteins form tonofilaments, a class of intermediate filament, in epidermis as well as in almost all other epithelia. The process of normal epidermal differentiation is characterized by a series of morphological and bio- chemical changes as cells progress from the germinative basal layer through the spinous and granular layers to the outer cornified layer. The 65 to 67 kD cytokeratins are reported to be present only above the basal layer, the 58 kD cytokeratin is reported to be expressed throughout the entire epidermis including the basal layer and the 56 kD cytokeratin is reported to be absent from the basal layer and is normally eliminated during stratum corneum formation. The 56 and 65 to 67 kD cytokeratins are reported to be characteristic of epidermal cells undergoing terminal differentiation and Normal human stomach: immunohistochemical staining for Muc-6 glycoprotein using NCL- may be considered as molecular markers for keratinization. MUC-6. Note cytoplasmic staining of mucus secreting cells of the deep glands. Paraffin section. Product Specific Information Clones AE1 and AE3 are specific for the 56.5, 50, 50', 48 and 40 kD acidic cytokeratins as well as the 65 to 67, 64, 59, 58, 56 and 52 kD basic cytokeratins. The cocktail of clones AE1 and AE3 exhibit broad reactivity with two families of cytokeratin, acidic and basic. Refer to page 34 for the Bond ready-to-use format.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. yoeais5 ,8ad1.Teepout r okal fmonoclonal of in cocktails expressed are be to products tissues. reported epithelial These all cytokeratins almost 18. recognize human to and designed with 8 antibodies 6, react 5, RTU-CK5/6/8/18 cytokeratins and NCL-L-CK5/6/8/18 NCL-CK5/6/8/18, Information Specific Product RTU-CK5/6/8/18 ready-to-use mL 7 NCL-L-CK5/6/8/18 liquid mL 1 NCL-CK5/6/8/18 lyophilized mL 0.1 mL, 1 LP34 Clone 5D3, (5/6/8/18) Multi-Cytokeratin format. ready-to-use Bond the for 27 page to Refer NCL-CK34 34 Clone 1/5/10/14 Multi-Cytokeratin 18. and 13 10, 8, 6, 5, simple 4, non- cytokeratins human also and with react cornifying to and reported of is NCL-C11 epithelia layers suprabasal neoplastic and a epithelium. and between squamous basal cornifying expressed both reactive differentially are and normal, 18 epithelium and 13 of 10, 8, variety 6, 5, 4, Cytokeratins NCL-C11 lyophilized mL 1 C-11 Clone (4/5/6/8/10/13/18) Multi-Cytokeratin cells. myoepithelial glandular section. of Paraffin staining cytoplasmic intense cytokeratins Note for RTU-AE1/AE3. staining using immunohistochemical prostate: of hyperplasia nodular benign Human LBn ed-ouePA0134 ready-to-use Bond mL 7 RTU-CK34BE12 ready-to-use mL 7 NCL-CK34BE12 lyophilized mL 1 el fnra rat oefliua ptei fnra hri n some bowel. large and and thyroid small normal normal the of of epithelia mesothelium ductal follicular and and some epithelia It acinar breast, some liver. pancreas, normal normal normal of in the squamous cells ducts of bronchial cells with bile ductal pneumocytes, and react with some lung reacts to also normal skin, reported in normal is mesothelium in and antibody epithelium ducts The sweat 14. and and epithelium 10 5, 1, proteins 1 ecswt ua yoeai nemdaefilament intermediate cytokeratin human with reacts E12 E12 (HIER) P F (Enzyme) P F (Enzyme) P HE)W (HIER) P F (HIER) P F (Enzyme) P F (Enzyme) P F rti NCL-MRP3 3 Protein Resistance-associated Multidrug lyophilized mL 1 DTX1 Clone NCL-MRP1 1 Resistance-associated Protein Multidrug lyophilized mL 0.1 mL, 1 33A6 Clone Antibodies Protein Resistance-Associated Multidrug cells. malignant of for section. staining staining Paraffin cytoplasmic immunohistochemical intense mouth: Note the NCL-L-CK5/6/8/18. of using floor cytokeratins the of carcinoma cell squamous Human sn C-R1 oectpamcsann fgauoaadtea el.Prfi section. Paraffin cells. thecal and granulosa of staining cytoplasmic 1 Note protein NCL-MRP1. resistance-associated using multidrug for staining immunohistochemical ovary: Human adrenal especially liver, tissues, in all observed pancreas. expression almost and kidney. ovary lower in the placenta, with gland, expressed in and brain levels be and lower to muscle etoposide at skeletal reported and is pancreas MTX, colon, MRP3 mRNA indicated liver, MRP5 has in as tissues expressed human be such various to of the blotting drugs Northern in teniposide. kD in expressed 200 effective chemotherapeutic transporter to a anion been 190 organic a transporting an has is is is MRP3 which protein protein mucosa. membrane respiratory The salivary MRP1 integral nasal brain, of spleen. placenta, glands 6. peripheral and and in cell epithelium and detected uterus to of testis not liver, variety lung, was MRP1 a gland, in but in demonstrated cells designated expressed been mononuclear is blood has members and mRNA family acids MRP1 6 amino types. gene 1531 (MRP) least of phosphoprotein protein at resistance-associated contains multidrug human The W P F etr blotting Western Paraffin Frozen (HIER) P (HIER) P C I lwcytometry Flow Immunofluorescence O E te applications Other lcrnmicroscopy Electron 137 /
Primary Antibodies Muramidase (Lysozyme) Polyclonal 1 mL lyophilized NCL-MURAM P (Enzyme) W 7 mL Bond ready-to-use PA0391 P (HIER) New! Intracellular muramidase, also known as lysozyme, has been reported to be expressed in myeloid and monocytic cells, in leukocytes and in myelo- proliferative disorders. Muramidase is also reported to be expressed in poorly differentiated leukemic monoblasts. Refer to page 35 for the Bond ready-to-use format. Human leiomyosarcoma: immunohistochemical staining for muscle specifi c actin using See also Lysozyme (Muramidase) on page 129. NCL-L-MSA-594
Mycobacterium tuberculosis
Clone 1.1/3/1 1 mL lyophilized NCL-MT W The re-emergence of tuberculosis itself, the significant increase in non- tuberculous mycobacterial infections in the immunosuppressed and the changing epidemiology of childhood mycobacterial lymphadenitis make it important to confirm the mycobacterial etiology of tissue granulomata Human spleen, myeloid leukemia: immunohistochemical staining for muramidase (lysozyme) using NCL-MURAM. Note intense cytoplasmic staining of myeloid cells. Paraffin section. whenever possible. Product Specific Information NCL-MT is raised to the 38 kD antigen of the mycobacterium tuberculosis complex (MTBC). NCL-MT reacts with several mycobacterial species, in Primary Antibodies Muscle Specific Actin Western blotting, including Mycobacterium tuberculosis, other members of the MTBC and Mycobacterium avium-intracellulare. Clone SC28 New! 1 mL, 0.1 mL liquid NCL-L-MSA-594 PW Clone HHF35 Myelin Basic Protein 1 mL lyophilized NCL-MSA FPW 7 mL Bond ready-to-use PA0258 P Clone 7H11 1 mL, 0.1 mL lyophilized NCL-MBP P (HIER) Muscle Specific Actin (MSA) is a highly conserved, ubiquitous protein found in muscle and some non-muscle cells. Actins can be divided into three Myelin basic protein is reported to account for about 30 percent of the subsets, alpha actins found in muscle tissue cells, beta and gamma actins proteins in myelin found in the central nervous system. It can induce found in non-muscle cells and a small subset of gamma actins also found in experimental allergic encephalomyelitis (EAE), a T-lymphocyte mediated muscle tissue cells. In normal tissues, expression is found in striated fibers disease due to delayed-type hypersensitivity; though each animal species of skeletal muscle, smooth muscle in arteries, veins and pericytes of smaller appears to respond to a different fragment of the 170 amino acid arteries, muscle in bowel, myometrium of the uterus, prostatic stroma, polypeptide. Four different isoforms have been identified through cDNA capsule cells of liver, kidney, lymph node and spleen, the myoepithelial cloning. All four of these variants are identical except for the insertion or layers of mammary ducts and glands, eccrine sweat glands and salivary deletion of two peptide fragments encoded by exons 2 and 5. Myelin basic glands. Expression is not found in epithelial cells, lymphoid cells, protein is reported to be expressed in oligodendrocytes, myelin of white macrophages, connective tissue and neuronal cells. In neoplastic tissues, matter in the brain and spinal cord and in peripheral nerves, though it is expression can be found in soft tissue tumors with muscle differentiation expressed less abundantly in gray matter. e.g. leiomyomas, leiomyosarcomas and rhabdomyosarcomas of varying Product Specific Information subtypes.Non-muscle sarcomas, carcinomas, melanomas and lymphomas do not express muscle specific actin. NCL-MBP was raised to guinea pig myelin basic protein and is reactive with human myelin basic protein. Product Specific Information NCL-L-MSA-594 was developed to outperform NCL-MSA in immunohistochemistry. Refer to page 35 for the Bond ready-to-use format.
Human spinal cord, cervical: immunohistochemical staining for myelin basic protein using NCL-MBP. Note cytoplasmic staining of cells in white matter. Paraffin section.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. ylprxds sn C-YL.Nt nes yolsi tiigo ainn myeloid malignant section. of Paraffin staining cells. cytoplasmic intense Note NCL-MYELO. for using staining myeloperoxidase immunohistochemical sarcoma: granulocytic marrow, bone Human format. ready-to-use Bond the for 36 page the to liver. in Refer the granulocytes of myelocyte cells of the Kupffer precursors in from neutrophil and in marrow in detectable blood, bone expressed in not be monocytes is to and reported and granulocytes is cell Myeloperoxidase the onwards. decreases granulocytes of stage level mature whose maturation including mRNA, the myeloperoxidase cells, to with myeloid marker superior all a in reacts is and component to HOCI reported major neutrophils. is a Myeloperoxidase by chloramines. be cytotoxic produced form be to most proteins the to with is known HOCI oxidant. oxidant strong granules a and bacteriocidal (HOCI), compounds cytoplasmic acid aromatic hypochlorous of azurophilic to variety ions a of chloride oxidise activated to constituent peroxide by major hydrogen uses generated a that peroxide is hydrogen series It myeloid the the phagocytes. of of most cells in metabolises found which enzyme lysosomal a is Myeloperoxidase PA0491 ready-to-use Bond mL 7 NCL-MYELO lyophilized mL 0.1 mL. 1 59A5 Clone Myeloperoxidase 63. page on Marker) (Myeloid BM2 also See NCL-BM2 lyophilized mL 1 BM-2 Clone (BM2) Marker Myeloid 63. page on Marker) (Myeloid BM1 also See NCL-BM1 lyophilized mL 1 BM-1 Clone (BM1) Marker Myeloid (HIER) P F FP (HIER) P P New! C--y-.Nt tiigo rprino uo elnce.Prfi section. Paraffin nuclei. cell tumor of proportion a of staining using Note protein NCL-L-Myf-4. Myf-4 for staining immunohistochemical rhabdomyosarcoma: Human format. ready-to-use Bond the with for 36 together page cells. Myf-6. product, to differentiated Refer of gene and nucleus Its the Myf-5 in myogenin. myogenin, accumulates of Myf-3, MyoD1, of homolog that human including the genes by is regulatory controlled Myf-4 is of cells myogenic set of affect a Differentiation primarily and adults. genes young muscle-specific and of sarcomas children number tissue a soft express myoblast-derived usually of that class a are Rhabdomyosarcomas PA0226 ready-to-use Bond mL 7 NCL-L-Myf-4 liquid mL 1 NCL-Myf-4 lyophilized mL 0.1 mL, 1 LO26 Clone (Myf-4) Myogenin C-YGOI.Nt yolsi tiigo ucefbr.Prfi section. Paraffin fibers. muscle of staining using cytoplasmic myoglobin Note for NCL-MYOGLOBIN. staining immunohistochemical muscle: skeletal adult Human format. ready-to-use Bond the for an 36 as in page functions to expressed Refer and be muscle smooth to in reported pigment. not transporting is but oxygen muscle Myoglobin cardiac group. and that heme skeletal acids amino single 153 of a polypeptide contains chain single cytoplasmic, a is Myoglobin PA0727 ready-to-use Bond mL 7 NCL-MYOGLOBIN lyophilized mL 0.1 mL, 1 MYO18 Clone Myoglobin W P F etr blotting Western Paraffin Frozen C I HE)W (HIER) P lwcytometry Flow Immunofluorescence (HIER) P (HIER) P HE)W (HIER) P PW O E te applications Other lcrnmicroscopy Electron 139 /
Primary Antibodies Myosin Heavy Chain Antibodies Myotilin
Clone S131 Clone RSO34 1 mL, 0.1 mL lyophilized Myosin Heavy Chain 1 mL lyophilized NCL-MYOTILIN F P (HIER) (smooth muscle) NCL-MHC-Sm F P (HIER) The myotilin gene on chromosome 5q31 encodes a 498 amino acid 7 mL Bond ready-to-use PA0493 P (HIER) polypeptide with a molecular weight of 57 kD. Myotilin is a structural protein of sarcomeric Z discs and sarcolemma in human skeletal and cardiac Clone RNMy2/9D2 muscle. It is homologous to palladin and titin in the two C-terminal Ig- domains and also to palladin in its unique serine-rich N-terminal region. 1 mL, 0.1 mL lyophilized Myosin Heavy Chain (developmental) Myotilin interacts with alpha-actinin, actin and gamma-filamin. A missense NCL-MHCd F mutation in the myotilin gene is associated with limb-girdle muscular dystrophy 1A (LGMD1A), an autosomal dominant disease characterized by Clone WB-MHCf proximal limb weakness. It is highly conserved between human and mouse 1 mL, 0.1 mL lyophilized Myosin Heavy Chain (fast) with its expression being more widespread in the embryo than in the adult. Expression of myotilin has been reported in adult skeletal and cardiac NCL-MHCf F muscle with variable expression reported in the peripheral nervous system, lung, liver and kidney. Clone WB-MHCn 1 mL, 0.1 mL lyophilized Myosin Heavy Chain (neonatal) NCL-MHCn F Clone WB-MHCs 1 mL, 0.1 mL lyophilized Myosin Heavy Chain (slow) NCL-MHCs F
Myosin is a contractile muscle specific protein composed of two heavy and four light chains. The myosin heavy chain has many isoforms which are specific for different muscles or fiber types, some of which are Primary Antibodies Human skeletal muscle: immunohistochemical staining for myotilin using NCL-MYOTILIN. Note developmentally regulated. Smooth muscle myosin heavy chain (SM-MHC) intense staining of muscle fibers. Paraffin section. is a cytoplasmic structural protein that is a major component of the contractile apparatus in smooth muscle cells. It has been reported to be specific for smooth muscle development. Refer to page 36 for the Bond ready-to-use format. Napsin A
Clone IP64 1 mL, 0.1 mL liquid NCL-L-NapsinA P (HIER) Reference Range Napsin A has a specific function in normal alveolar epithelium and is proposed to play a role in the proteolytic processing of surfactant precursors. Napsin A is reported to be predominantly expressed in lamellar bodies of type II pneumocytes, secondary lysosomes of alveolar macro- phages, respiratory epithelium of terminal and respiratory bronchioles, plasma cells, within a subset of lymphocytes in normal lung as well as in epithelial cells of renal tubules in normal kidney and is weakly expressed in normal spleen. Past studies have also reported that Napsin A is expressed in Human prostate: immunohistochemical staining for myosin heavy chain using NCL-MHC-Sm. Note staining of smooth muscle fibers. Paraffin section. 90 percent of primary lung adenocarcinomas.
Human lung adenocarcinoma: immunohistochemical staining for napsin A using NCL-L-Napsin A. Note punctate cytoplasmic staining of malignant cells and infiltrating macrophages. Paraffin section.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. e loC5 NA)o ae81. page on (NCAM) CD56 also See RTU-CD56-1B6 ready-to-use mL 7 NCL-L-CD56-1B6 liquid mL 1 NCL-CD56-1B6 lyophilized mL 0.1 mL, 1 1B6 Clone NCL-CD56-564 lyophilized mL 0.1 mL, 1 CD564 Clone (CD56) NCAM section. Paraffin cells. sertoli of staining Note membrane NCL-L-N-Cad. and using cytoplasmic N-Cadherin for staining immunohistochemical testes: Human differentiate to antibodies of panel a in been marker has N-Cadherin useful adenocarcinomas. from associated prognosis. mesotheliomas a is poor as which and transition, invasion proposed mesenchymal tumor to increased the of in epithelial with one the is N-Cadherin expression implicated of E-Cadherin increased of features are loss progression the myocardial tumor with neural, and concomitant During including expression types cells. adhesion, cell mesenchymal various is via cell-cell in and N-Cadherin a expressed development. cell be in during to tissues reported the repeats, of Cadherins role aggregation catenins. and of important the segregation five including an components proteins play cytoskeletal of intracellular with with interactions consisting which domain, interactions cytoplasmic conserved mediates domain, highly a and domain transmembrane calcium-binding extra manner. an specific comprising tissue structure a characteristic cellular VE- a in and have expressed P cadherins cell be R, classical to dependent The N, believed E, calcium are the of which include family Cadherins cadherins cadherin classical the The molecules. of adhesion member a is N-Cadherin NCL-L-N-Cad liquid mL 0.1 mL, 1 IAR06 Clone N-Cadherin HE)W (HIER) P (HIER) P (HIER) P HE)W (HIER) P (HIER) P C-GRi asdt h p5lwafnt GRprotein. NGFR low-affinity gp75 the to raised is NCL-NGFR Information Specific Product and paragangliomas differentiation. sheath phaeochromocytomas, lymphocyte to nerve reported of a peripheral been of proportion and also tumors a has vessels NGFR in sheath blood node. expressed root lymph mature be and outer of spleen glands, the adventitia in sweat follicles, subpopulation and hair salivary cells of includes Schwann that breast, cells cells axons, of non-neural neuronal in cells and in nerves myoepithelial expressed peripheral death. of be cell cells gp75-induced to perineural unbound inhibit and reported gp75 to vitro, is of shown In binding been (gp75) surface whereas, has elucidated. NGFR antibody death, fully cell or cell ligand neural been distinctive NGF promote yet by to two shown not been of has has The (gp75). gp75 binding gp75 NGFR of low-affinity the the role and by ) functional (TrkA mediated NGFR high-affinity growth is the receptors; neurons Nerve action of receptors. survival its and of differentiation and development, factor superfamily the growth for (TNF) important nerve is the factor factor of necrosis member a tumor is (NGF) (NGFR) receptor factor growth Nerve NCL-NGFR lyophilized mL 0.1 mL, 1 7F10 Clone (gp75) Receptor Factor Growth Nerve nyeperamn a nac tiigi oecases. some in staining enhance Pathol. may pretreatment Surg Enzyme J Am Information al., Specific Product et M (Miettinen (1998)). negative 22(3):327-32 and NB84 tumors, Wilms’ were rhabdomyosarcomas, lymphomas including other tumors All tumors. cell these round markers in small neural synaptophysin) other and of the CD57, expression more However, proteins, the (neurofilament with sarcoma. slightly correlate Ewing’s not did seen in positivity NB84 than peripheral was tumors of reactivity cases neuroectodermal (rosette-positive) numbers primitive NB84 defined smaller morphologically The in desmoplastic in usually commonly of neuroblastomas. cells, percent positive the 50 showed and than and in tumors cases cell of neuroblastoma skeletal round react- percent small NB84 20 of neuroblastoma, showed approximately medulloblastoma identification in to ivity and the addition sarcoma Ewing’s in In extraskeletal specific, tissue. sensi- more less formaldehyde-fixed was 83 NB84 all although synaptophysin, and with tumors tive, 22) these Compared of of positive. none (21 CD99 but useful were study NB84, a with their reacted be in neuroblastomas to differentiated neuroblastomas Most antibody tumors. undifferentiated cell of monoclonal the round small NB84 of other because from the neuroblastoma study, cancers separate indicated to major al., reagent pediatric a et In among M excretions. Mietten unique catecholamine and is neural regressions maturing spontaneous cells, pathologically progenitor from the derived crest of tumor system, This children. nervous in sympathetic disease malignant complex a is Neuroblastoma NCL-NB84 lyophilized mL 1 NB84a Clone Marker Neuroblastoma membrane section. and Paraffin cytoplasmic neurons. Note cholinergic NCL-NGFR. of using staining (gp75) staining receptor immunohistochemical factor forebrain: growth basal nerve in for neurons Meynert of nucleus brain, Human W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence FPC (HIER) P O E te applications Other lcrnmicroscopy Electron 141 /
Primary Antibodies Neurofilament Antibodies Neuron Specific Enolase
Clone DA2 Clone 22C9 1 mL lyophilized Neurofilament 68 kD 1 mL lyophilized NCL-NSE-435 PW NCL-NF68-DA2 F P (HIER) 7 mL Bond ready-to-use PA0435 P (HIER) Clone NR4 Clone 5E2 1 mL lyophilized Neurofilament 68 kD 1 mL lyophilized NCL-NSE2 FPW NCL-NF68 F P (HIER) 1 mL liquid NCL-L-NSE2 FPW 7 mL ready-to-use RTU-NSE2 FP Clone RT97 1 mL lyophilized Neurofilament 200 kD Enolase is a glycolytic enzyme catalysing the reaction pathway between 2- phosphoglycerate and phosphoenol pyruvate. In mammals, enolase NCL-NF200 FP molecules are dimers composed of three distinct subunits (, and ) whereas, in rats, five forms have been found. The subunit and subunit Clone N52.1.7 are of approximately 47 kD and 45 kD, respectively. The and enolases 1 mL lyophilized Neurofilament 200 kD are located mainly in the nervous tissue and neuroendocrine cells. NCL-NF200-N52 F P (HIER) Product Specific Information 7 mL Bond ready-to-use PA0371 P (HIER) New! Clone 22C9 was developed to produce superior staining on paraffin sections. Neurofilaments constitute the main structural elements of neuronal axons Clone 22C9 reacts with the subunit of the enolase isoenzyme. Clone 5E2 and dendrites. Neurofilaments are composed of three major subunits reacts with the 47 kD component of the gamma-gamma enolase isoenzyme. referred to as the neurofilament triplet, with molecular weights of 68 kD, 160 kD and 200 kD. Neurofilament subunits are reported to be present in Refer to page 37 for the Bond ready-to-use format. neurons, neuronal processes, peripheral nerves and sympathetic ganglion cells. Within tumors, only neoplastic cells of neural origin or those exhibiting neuronal differentiation, have been reported to express neurofilaments. Refer to page 37 for the Bond ready-to-use format. Nitric Oxide Synthase Antibodies Primary Antibodies Clone NOS-125 1 mL lyophilized Nitric Oxide Synthase-1 NCL-NOS-1 P (HIER) Clone RN5 1 mL lyophilized Nitric Oxide Synthase-3 NCL-NOS-3 F P (HIER) Human nitric oxide synthases are a family of enzymes responsible for the synthesis of nitric oxide from L-arginine and molecular oxygen. There are at Human spinal cord, lumbar: immunohistochemical staining for the 68 kD neurofilament using least three nitric oxide synthases; NOS-1, also known as neuronal NOS or NCL-NF68-DA2. Note intense cytoplasmic staining of perikarya and neuronal axons. Paraffin nNOS, NOS-2, which is referred to as inducible NOS or iNOS and NOS-3, section. also known as endothelial NOS or eNOS. As suggested by their nomen- clature, these enzymes have different cellular distribution and are subjected to different regulatory mechanisms. NOS-3 is reported to be constitutively expressed and produces picomolar quantities of nitric oxide (NO) which play a role in signal transmission resulting in physiological effects. In the gastrointestinal tract, NO is reported to play a protective role where it has direct microbiocidal properties and acts as a first line of mucosal defence in the stomach. The function of NO in tumor development, promotion and progression is unclear. The effects may be both beneficial but also detrimental to those individuals with gastric cancer where it is reported that NO supports tumor progression through the creation of neovasculature.
Human small intestine: immunohistochemical staining for nitric oxide synthase-1 using NCL-NOS-1. Note cytoplasmic staining of enteric ganglia. Paraffin section.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. C-u8.Nt eiula n yolsi tiigo ainn el.Prfi section. Paraffin cells. malignant of staining cytoplasmic using and kD) perinuclear (88 Note nucleoporin NCL-Nup88. for staining immunohistochemical carcinoma: breast Human weakly either be to benign reported is hyperplasia, Nup88 In tissues, undetectable. distribution. overexpressed or normal expressed be cytoplasmic nuclear most to the and and reported at tumors perinuclear in is localized a protein overexpressed is the it be with lines, malignancies, to cell In reported In membrane. widely is development. a and that as oncogenesis regarded marker be of proliferation may subcomplex Nup88 dynamic distributed 214. a with CAN/Nup associated nucleoporin (Nup). is nucleoporins oncogenic (Nup88) called proteins nucleoporin 100 kD to 88 50 The of up nuclear structures made vertebrates, are macromolecular complexes In pore traffic. elaborate nucleocytoplasmic large, bidirectional mediate are that complexes pore Nuclear NCL-Nup88 lyophilized mL 1 JRC1 Clone kD) (88 Nucleoporin 118. page on (NS3) virus C Hepatitis also See NCL-HCV-NS3 lyophilized mL 0.1 mL, 1 MMM33 Clone virus) C (Hepatitis NS3 homolog. H2 the with weakly only and strongly H1 with reacts NCL-nm23 of process metastatic Information Specific the Product of metastasis- control are the nm23-H2 cells. in malignant and diphosphate implicated nm23-H1 nucleoside genes respectively. designated and suppressor (NDPK-B), and (NDPK-A) B as Two isolated A identified kinase been potential. been kinase have genes have metastatic diphosphate mouse- these of gene low nucleoside of products nm23 The and screening nm23-H2. the and high differential nm23-H1 of of by homologs lines identified human cell first melanoma was derived gene nm23 The NCL-nm23 lyophilized mL 1 37.6 Clone Protein nm23 FPW HE)W (HIER) P (HIER) P F c- n O. xrsinrpeet oe ehns for mechanism novel format. a ready-to-use Bond the for represents 38 page to Refer expression cells. both RS of BOB.1 in deregulation absence octamer gene the immunoglobulin between BOB.1, and interaction and Oct-2 an factors Oct-2 requires immuno- transactivating also express the Ig cells not and As genes. sites B do Ig the cells in within cases, expression mutations crippling these gene of of presence the number to a due the globulin In germinal represent in from cells. derived cells are B greater and (RS) center disease Sternberg significantly Hodgkin's classical Reed also in be expression. cells lymphomas malignant of cell to levels B reported other high that although although display is lymphomas, indicate cells, derived expression Reports center B germinal BOB.1/OBF.1. Oct-2 cells. and Oct-2 to these addition, for expression in restricted In higher shows higher cells not B much BOB.1/ center is germinal as are such expression levels coactivators is restricted protein expression It cell genes. Oct-2 B specific cell of family B OBF.1. activity regulating homeo-domain the sites POU octamer on the gene dependent Ig to the belonging to factor binds that transcription a is Oct-2 PA0532 ready-to-use Bond mL 7 NCL-OCT2 lyophilized mL 0.1 mL, 1 Oct-207 Clone Oct-2 c-/.Nt nes tiigo lrptn uo el.Prfi section. Paraffin cells. tumor pluripotent of NCL-L- staining using intense factor Note transcription Oct-3/4. Oct-3/4 for staining immunohistochemical seminoma: Human some for origin format. ready-to-use Bond cell the for 38 and germ exhibit page to Refer which a seminoma/dysgerminoma tumor. tumors primary establishing uncertain cell of including tumors and germ metastatic for pluripotentiality, carcinoma, critical marker A embryonal of useful cells. has a germ Oct3/4 features pluripotency. and as of loss cells proposed with associated stem been are levels embryonic Oct3/4 factors, by of transcription amount of expressed family homeodomain is POU the which of member a is Oct3/4 PA0934 ready-to-use Bond mL 7 NCL-L-Oct3/4 liquid mL 0.1 mL, 1 N1NK Clone Oct-3/4 section. Paraffin cells. B center germinal mainly of NCL-OCT2. staining using nuclear product intense gene Note Oct-2 for staining immunohistochemical tonsil: Human W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence HE)W (HIER) P (HIER) P (HIER) P (HIER) P F New! O E Reference Range te applications Other lcrnmicroscopy Electron 143 /
Primary Antibodies Osteonectin
Clone 15G12 1 mL, 0.1 mL lyophilized NCL-O-NECTIN P (HIER) W Osteonectin (ON), also known as BM-40 or SPARC (secreted protein, acidic and rich in cysteine) is a multifunctional glycoprotein (32.5 kD) involved with tissue mineralization as well as extracellular matrix modelling. ON is the most abundant glycoprotein secreted by human osteoblasts in developing bone and odontoblasts of developing teeth. ON mRNA and protein have been reported to be expressed in non-mineralized tissues such as steroid- producing cells of the adrenal glands, suprabasal layers of the epidermis, Human osteosarcoma: immunohistochemical staining for osteopontin using NCL-O-PONTIN. glomeruli in the kidney, bronchi of the lung, megakaryocytes and large Note extracellular staining in close proximity to tumor cells. Paraffin section. vessels. This organ-specific distribution of ON in non-mineralized tissues suggests a role during human development. It is also reported to be expressed in osteosarcomas, at high levels in osteoblastic osteosarcomas and at low levels in osteoid formation of the diffuse deposition type. Ovarian Cancer Antigen (CA125)
Clone Ov185:1 1 mL lyophilized NCL-CA125 F P (HIER) 1 mL liquid NCL-L-CA125 F P (HIER) 7 mL ready-to-use RTU-CA125 F P (HIER) 7 mL Bond ready-to-use PA0539 P See also CA125 (Ovarian Cancer Antigen) on page 64.
Human osteosarcoma: immunohistochemical staining for osteonectin using NCL-O-NECTIN. Primary Antibodies Note cytoplasmic staining of malignant cells. Paraffin section. OX40 (CD134)
Clone 102H6 Osteopontin 1 mL lyophilized NCL-CD134 F P (HIER) See also CD134 (OX40) on page 86. Clone OP3N 1 mL, 0.1 mL lyophilized NCL-O-PONTIN P (HIER) Osteopontin is a 34 kD extracellular matrix protein with a cell binding domain. Other molecules which share this domain include fibronectin, p21 (WAF1 Protein) vitronectin and a variety of other extracellular proteins that bind members of the integrin family of cell surface receptors. Osteopontin was originally Clone 4D10 identified as a major component of the non-collagenous organic bone 1 mL, 0.1 mL lyophilized NCL-WAF-1 P (HIER) matrix, however, it has subsequently been demonstrated in a wide range of normal adult tissues and body fluids. It is a multifunctional protein involved 1 mL liquid NCL-L-WAF-1 P (HIER) in bone mineralization, cell adhesion, cell migration, chronic inflammatory See also WAF1 Protein (p21, C1P1) on page 170. disease and transformation. Osteopontin is reported to be linked to tumorigenesis and metastasis in several experimental animal models and human cancers. In breast carcinomas, demonstrated by RT-PCR and in situ hybridization studies, expression was confined to tumor cells. It is also reported to be expressed in normal breast, including vascular endothelial p27 Protein cells, macrophages, myoepithelial cells, osteosarcomas but not in lymphoid tumors. Other studies using in situ hybridization have shown expression in the epithelium of gastrointestinal tract, gall bladder, pancreas, urinary and Clone 1B4 reproductive tracts, lung, salivary and sweat glands. Ganglion cells in the 1 mL, 0.1 mL lyophilized NCL-p27 P (HIER) W bowel also express osteopontin as do macrophages, T cells and NK cells p27 protein, also known as kinase inhibitory protein 1 (Kip1), binds to cyclin upon activation. Expression of osteopontin in vascular smooth muscle and E/cdk2 complexes (but not to cdk2 alone) and is detected in purified extracts endothelium may be triggered by atherosclerosis, vascular calcification and of growth-arrested cells. p27 protein constrains cell proliferation by setting by hypertension. the threshold level of cyclin E necessary to activate cdk2. The 27 kD protein is also present in proliferating cells but only in a sequestered form when it is unavailable to interact with cyclin E/cdk2 complexes. It is likely that cyclin D complexed with catalytically inactive cdk4 is sufficient to sequester p27 protein and titrate its function. The presence of bound p27 protein in proliferating cells suggests that its role may not be restricted to inducing cell cycle arrest but to also set the cyclin E threshold for execution of the G1 to S phase transition during each mitotic cycle.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. C-5-81 oeitnencersann ftmrcls aafnsection. Paraffin cells. tumor of staining nuclear using intense protein Note p53 NCL-p53-1801. for staining immunohistochemical adenocarcinoma: colonic Human p53 human of forms mutant conditions. and non-denaturing type and denaturing wild under both protein recognizes 1801 PAb Clone Information breast Specific Product of proportion expression allelic high and of a protein protein. p53 site that p53 for frequent reported of immunostaining a been show carcinomas has 17p, colon It chromosome and tumors. on many located in is loss p53 for gene The NCL-p53-1801 lyophilized mL 0.1 mL, 1 1801 PAb Clone (1801) Protein p53 section. Paraffin cells. T3T3 of proportion a NCL-p53-505. of using staining protein nuclear mouse intense p53 Note for staining immunohistochemical cells: T3T3 Mouse format. ready-to-use Bond the the for 38 as page protein protein. to p53 Refer p53 mouse and mouse rat recombinant with reacts same It the NCL-p53-CM5p. polyclonal, to raised is NCL-p53-505 is Information protein Specific p53 Product human phenotypically and protein of p53 mouse levels mutations. in similar. very missense sites two high antigenic in carries of which range express not The protein p53 though cells protein type wild osteocytes, the p53 T3T3 characteristic by and mice, Mouse apoptosis irradiated thymocytes well hepatocytes. In is and splenocytes, vitro genes. arrest in in other growth accumulates damage of induce DNA regulation to to transcriptional appears response The cancer. in and of protein development established the p53 suppressing of in role accumulation vital a plays protein p53 PA0057 ready-to-use Bond mL 7 NCL-p53-505 lyophilized mL 1 IMX25 Clone Protein p53 HE)W (HIER) P (HIER) P HE)WC W (HIER) P F oeitnencersann ftmrcls aafnsection. Paraffin cells. tumor of NCL-p53-BP. staining using nuclear protein intense p53 Note for staining immunohistochemical carcinoma: breast Human cases. p53 some in human induced staining heat of improve The may forms technique conditions. mutant retrieval non-denaturing epitope and and type denaturing under wild protein both recognizes the BP53-12 Clone by apoptosis well is and Information Specific vitro arrest Product in growth damage genes. induce other DNA of to regulation to transcriptional appears response The cancer. in and of protein development established the p53 suppressing of in role accumulation vital a plays protein p53 NCL-p53-BP lyophilized mL 1 BP53-12 Clone (BP53-12) Protein p53 o 5 rti sn C-5-M.Nt nes ula tiigo rprino tumor of proportion a of staining section. nuclear Paraffin intense cells. Note NCL-p53-CM1. staining using immunohistochemical protein carcinoma: p53 breast for metastatic by infiltrated node lymph Axillary of DO-7. NCL- forms clone than conditions. mutant overfixation non-denaturing to and sensitive and less type denaturing is p53-CM1 wild under protein both p53 recognizes human antibody polyclonal This Information Specific Product mucosa. normal adenomas. in of be reported percent to been 9 has reported only expression and been No carcinomas has of in protein percent event 47 p53 genetic in tumors, commonest overexpressed colonic the In represent malignancy. may protein human p53 the of Mutation NCL-p53-CM1 lyophilized mL 0.2 Polyclonal (CM1) Protein p53 W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence FPW HE)W (HIER) P F O E te applications Other lcrnmicroscopy Electron 145 /
Primary Antibodies p53 Protein (CM5) p53 Protein (DO-7)
Polyclonal Clone DO-7 0.2 mL lyophilized NCL-p53-CM5p P (HIER) W 1 mL, 0.1 mL lyophilized NCL-p53-DO7 FPWC The accumulation of p53 protein in response to genotoxic stress in vitro is 1 mL liquid NCL-L-p53-DO7 FPWC Reference Range well established and appears to induce growth arrest and apoptosis by the 7 mL ready-to-use RTU-p53-DO7 FP transcriptional regulation of other genes and possibly by other direct mechanisms. 7 mL Bond ready-to-use PA0057 P (HIER) Product Specific Information Product Specific Information This monoclonal antibody recognizes both wild type and mutant forms of NCL-p53-CM5p is specific for mouse and rat p53 protein. human p53 protein under denaturing and non-denaturing conditions. The epitope recognized by clone DO-7 can be destroyed by prolonged fixation in buffered formalin. The heat induced epitope retrieval technique may improve staining in some cases. Refer to page 38 for the Bond ready-to-use format.
Western blot: detection of p53 protein (53 kD) using NCL-p53-CM5p. Lane A, molecular weight markers. Lane B, T3T3 mouse cell line immunoblotted with NCL-p53-CM5p.
Human skin, basal cell carcinoma: immunohistochemical staining for p53 protein using the p53 Protein (DO-1) ready-to-use reagent RTU-p53-DO7. Note intense nuclear staining of tumor cells. Paraffin Primary Antibodies section. Clone DO-1 1 mL lyophilized NCL-p53-DO1 F P (HIER) W In man, the p53 gene is located on the small arm of chromosome 17. Alterations of the p53 tumor suppressor gene are a common feature of p53 Protein (Phosphospecific) human malignancies. A normal function of this gene is to induce apoptosis after DNA damage and, therefore, its activation can permit the survival of Clone FP3-2 cells that have sustained genetic damage. 1 mL lyophilized NCL-p53-PHOS P (HIER) W Product Specific Information p53 protein plays a vital role in suppressing the development of cancer. Clone DO-1 recognizes both wild type and mutant forms of human p53 protein Post-translational modification by phosphorylation has been proposed to be under denaturing and non-denaturing conditions. The epitope to which NCL- an important regulatory mechanism of p53 function. Several p53 p53-DO1 maps is sited at the N-terminus at amino acids 20 – 25. phosphorylation sites fall within or closely flank a number of functional domains within the p53 molecule including transcriptional transactivating, DNA binding and oligomerization sequences. Several p53 protein mutants are less phosphorylated than wild type p53 protein when expressed in vivo which may suggest that phosphorylation positively regulates DNA binding. However, other experiments have also suggested that phosphorylation may also negatively regulate DNA binding. Product Specific Information NCL-p53-PHOS is specific for the phosphorylated site on human p53 protein at Ser392 within the non-specific DNA binding/oligomerization region. The functional significance of phosphorylation at this site only, remains unclear.
Human colonic adenocarcinoma: immunohistochemical staining for p53 protein using NCL-p53-DO1. Note intense nuclear staining of tumor cells. Paraffin section.
Human colonic adenocarcinoma: immunohistochemical staining for p53 protein using NCL-p53-PHOS. Note intense nuclear staining of tumor cells. Paraffin section.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. uos 0preto vra uosad3 ecn fhepatocellular of percent 30 and tumors tumors of ovarian number of a lung carcinomas. of in percent percent 80 blotting 50 carcinomas, western breast tumors, has of and/or percent expression relative 40 RT-PCR approximately Elevated including The epithelium. by in squamous shown low zeta. be normal been to eg reported and is tissues expression epsilon normal protein p73 and p73 transcriptional in p73 activity. delta, modulate suppression resulting may Alternative growth variant of species gamma, splice unclear. each mRNA of induction beta, level p73 is expression known development the alpha, six tumor least in isoforms; in at results produces function cells, splicing its tumor This p53-defective but promoters. in gene, apoptosis suppressor reported p53-responsive tumor protein the p73 activates of of homolog identified Overproduction first p53. the was protein p73 NCL-p73 lyophilized mL 1 24 Clone (alpha) Protein p73 protein fusion format. ready-to-use Bond recombinant p63 the for the 38 prokaryotic page of to isoforms Refer a six to to common (aa319-410) raised molecule. region a is to corresponding 7JUL altered is but Clone protein p63 adenocarcinoma cancer. Information prostate Specific human Product in carcinogenesis. bladder in in expressed event Ectrodactyly p53 mutated frequent be a the disease is rarely expression to to the is contrast not The gene with In reported morphogenesis. p63 syndrome. children epidermal Clefts the in and Facial gene, mutated naso- limb and is for Dysplasia in Ectodermal essential gene development expressed is p63 prostate abundantly it for human mice, required be in is to protein and, p63 reported The carcinomas. p53. an also pharyngeal of lacks domain is deltaNp63, transactivating the isotype, deltaN-isotype to dominant including corresponding the cell N-terminus tissues, squamous cells, acidic differentiated poorly mouse epithelial most In in and expressed protein carcinomas. be be p63 human to prostate. to reported and normal also urothelium is reported cervix, of epithelium, of is variety cells major proliferating protein also a six least and p63 in at (TAp63) for expressed (deltaNp63). encodes activities activities and death-inducing family dominant-negative gene transactivating, p53 with the of isotypes member a is p63 PA0478 ready-to-use Bond mL 7 NCL-p63 lyophilized mL 0.1 mL, 1 7JUL Clone Protein p63 126. page on Protein) (p57 Kip2 also See NCL-p57 lyophilized mL 0.1 mL, 1 25B2 Clone (Kip2) Protein p57 (HIER) P F (HIER) P (HIER) P F (HIER) P Reference Range e loAK(npatcLmhm iae C26 p0 npg 54. page on (p80) (CD246) Kinase) Lymphoma (Anaplastic ALK also See PA0306 ready-to-use Bond mL 7 NCL-ALK lyophilized mL 0.1 mL, 1 5A4 Clone (CD246) (ALK) Kinase) Lymphoma (Anaplastic p80 cells. epithelial basal NCL-p73. of using staining protein nuclear p72 Note for staining immunohistochemical cervix: human Normal pcfcfrHVtps6 1ad18. is and NCL-HPV-4C4 11 18. type 6, HPV types of HPV protein for coat specific L1 cervical, the for of specific is agents NCL-HPV18 causative the Information are Specific Product 18 and malignancies. 16 vulvar as HPV been and types such vaginal neoplasia. diseases but has benign cervical relatively warts with (HPV) developing associated genital been of Papillomavirus have 11 risk and human 6 increased types of an types with associated specific with Infection 11,18) 6, (types NCL-HPV-4C4 Papillomavirus lyophilized mL 0.1 mL, 2 4C4 Clone 18) (type NCL-HPV18 Papillomavirus lyophilized mL 0.1 mL, 2 5A3 Clone Antibodies Papillomavirus W P F etr blotting Western Paraffin Frozen (HIER) P F (HIER) P F C I lwcytometry Flow Immunofluorescence (HIER) P (HIER) P O E te applications Other lcrnmicroscopy Electron 147 /
Primary Antibodies Parathyroid Hormone
Clone 105G7 1 mL, 0.1 mL lyophilized NCL-PTH-488 P The parathyroid glands are small, oval, endocrine glands closely associated with the thyroid gland. The parathyroid glands regulate serum calcium and phosphate levels via parathyroid hormone (parathormone). Parathyroid hormone raises serum calcium levels directly, by increasing the rate of osteoclastic reabsorption and promoting breakdown of the bone matrix, and indirectly, by increasing the renal tubular reabsorption of calcium ions and inhibiting the reabsorption of phosphate ions from the glomerular filtrate, Human brain, cerebellum: immunohistochemical staining for alpha parvalbumin using NCL- and finally, by promoting the absorption of calcium from the small intestine. PARVALBUMIN. Note cytoplasmic staining of Purkinje, basket and stellate cells. Paraffin Parathyroid hormone is the most important regulator of blood calcium levels section. and is essential to life, whereas calcitonin appears only to provide a complementary mechanism for fine adjustment. Chief cells are the most abundant cells in the parathyroid gland and are responsible for the secretion of parathyroid hormone. Antibodies to parathyroid hormone together with antibodies to thyroglobulin are useful in studies to differentiate Parvovirus B19 parathyroid-derived lesions from thyroid-derived lesions. Clone R92F6 1 mL, 0.1 mL lyophilized NCL-PARVO FP Parvovirus B19 is a small, single-stranded DNA virus which causes erythema infectiosum also known as ‘slapped cheek syndrome'. Clinically, this is a febrile disease in children, often epidemic, with a facial maculopapular rash causing flushed cheeks. In individuals with erythrocyte abnormalities, such as sickle cell anaemia, Parvovirus B19 can cause hemolytic complications where the virus replicates in bone marrow cells and inhibits erythropoiesis. Parvovirus B19 has also been implicated with spontaneous abortion in humans. Primary Antibodies Product Specific Information Human parathyroid: immunohistochemical staining for parathyroid hormone using NCL-PTH- NCL-PARVO is specific for the viral antigens, VP1 (84 kD) and VP2 (58 kD). 488. Note cytoplasmic staining of chief cells. Paraffin section.
Parvalbumin (Alpha)
Clone 2E11 1 mL lyophilized NCL-PARVALBUMIN P (HIER) Alpha and beta parvalbumins are low molecular weight, water-soluble, calcium-binding proteins. The protein is found in a subset of fast-spiking inhibitory GABAergic interneurons with a Ca2+ buffering capacity that reduces the Ca2+ -dependent K+ outward current. Unlike other Ca2+ binding Human fetal lung, post-mortem tissue: immunohistochemical staining for Parvovirus B19 using proteins, parvalbumin-containing neurons appear to co-localize only with NCL-PARVO. Note intense staining of infected cells within the capillaries. Paraffin section. corticotropin-releasing factor and not with other neuropeptides associated with GABA such as somatostatin, neuropeptide Y and cholecystokinin. Neurons which contain parvalbumin appear to be resistant to ischemia, epilepsy and N-methyl-D-aspartate receptor agonists due to their ability to buffer increase in intracellular calcium. Alpha and beta parvalbumins are Pax-5 reported to be expressed in different human tissues with the alpha form highly expressed in extracts of human cerebellum, weakly in kidney and not Clone 1EW in skeletal muscle, thymus, lung, placenta, heart, liver and diaphragm. The 1 mL liquid NCL-L-PAX-5 P (HIER) W beta form of parvalbumin has been detected only in preterm placenta. These expression patterns differ significantly between human and rodent species 7 mL Bond ready-to-use PA0552 P (HIER) with these differences also reflected with some members of the S-100 family Pax genes are a family of developmental control genes that encode nuclear of Ca2+ binding proteins. Within the cerebellum, alpha parvalbumin is transcription factors and have been implicated in the control of mammalian reported to be localized to Purkinje, basket, stellate and Golgi cells. In cases development. PAX-5 is a B cell specific transcription factor that is of spinocerebellar ataxia-1 (SCA-1), the number of Purkinje cells expressing expressed in pro B cells, pre-B and mature B cells, and subsequently in all alpha parvalbumin is reported to be much reduced, which may reflect stages of B cell development until the plasma cell stage in which it is biochemical changes preceding Purkinje degeneration. downregulated. Product Specific Information Product Specific Information
NCL-PARVALBUMIN does not detect parvalbumin in preterm placenta The use of H2O2 to block endogenous peroxidase has been shown to have a indicating its specificity for the alpha form of this protein. detrimental effect on the epitope recognized by Clone 1EW It is, therefore, critical that blocking with H2O2 should be carried out after application of the primary antibody with solutions of no greater than 3 percent, otherwise staining intensity will be reduced. Refer to page 39 for the Bond ready-to-use format.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. e loC3 PCM1 npg 77. page on (PECAM-1) CD31 also See PA0250 ready-to-use Bond mL 7 NCL-CD31-1A10 lyophilized mL 0.1 mL, 1 1A10 Clone (CD31) PECAM-1 section. Paraffin cytotrophoblasts. of staining membrane NCL-L-P-Cad. intense using Note P-cadherin for staining immunohistochemical placenta: Human Ca a is E-cadherin, like P-cadherin, NCL-L-P-Cad liquid mL 1 NCL-P-Cad lyophilized mL 1 56C1 Clone P-Cadherin section. Paraffin cells. B of staining using nuclear Pax-5 Note for NCL-L-PAX-5. staining immunohistochemical leukemia: lymphoblastic acute Human fPcdei xrsinhv lobe eotdi ratadmelanocytic and breast in final reported pattern cancers. the been the also in in have involved Changes expression carcinomas. P-cadherin be epidermal of and in phenotype progression contribute tumor epithelial may of the stage P-cadherin of epithelia. maintenance the stratified in the multicellular P-cadherin of detected to only of layers adhesion. is parabasal protein cell integrity the or to and basal the restricted cell be to selective maintaining reported for is in expression responsible role is It fundamental structures. a has and HE)W (HIER) P F HE)W (HIER) P F 2+ dpnetcl deinmolecule adhesion cell -dependent (HIER) P (HIER) P ua olclrlmhm:imnhsohmclsann o efrnuigNCL-PERFORIN. using perforin for staining immunohistochemical lymphoma: follicular Human The cells. NK and cells immunosuppressive T the by gamma/delta blocked and in partially A. extent cyclosporin is lymphocytes drug mRNA lesser granular perforin a of positive to large induction CD8 but in positive induced cells significantly is CD3 T expression negative, This cells, CD3 region. cells. human T spanning NK gamma/delta cell in membrane expressed positive target constitutively the be the CD56 at to on reported particularly a is share homology lesions Perforin C9 of and circular Perforin degree complement. forms by high induced Perforin those to lyse. similar membrane to and antigens foreign them express which induce cytotoxic cells of to granules bind cytoplasmic CTLs in (CTLs). T-lymphocytes found protein pore-forming a is Perforin NCL-PERFORIN lyophilized mL 0.1 mL, 1 5B10 Clone Perforin oeitnectpamcsann fetrcgnlo el n erlelements. neural and cells section. ganglion Paraffin enteric of staining NCL-PERIPH. cytoplasmic using intense peripherin Note for staining immunohistochemical bowel: small Human as such tumors derived crest neural in expressed ganglioneuroblastomas. be and neuroblastomas is to the Peripherin reported disorders. of of (HD)-related or is also abnormalities cells disease ganglion expression highly demonstrate Hirschsprung's of density The in to the is importance of crest. used assessment is and The be neural system. nervous may Peripherin system the enteric peripherin of nervous cells. of derivatives peripheral absence ganglion neuronal developing in enteric the enriched expressed including is in that neurons, protein expressed filament intermediate peripheral III type in kD 57 a is Peripherin NCL-PERIPH lyophilized mL 1 PJM50 Clone Peripherin section. Paraffin lymphocytes. T cytotoxic occasional of staining granular focal Note W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence HE)W (HIER) P F (HIER) P O E te applications Other lcrnmicroscopy Electron 149 /
Primary Antibodies PETA-3 (CD151) Plasma Cell Marker
Clone RLM30 Clone LIV3G11 1 mL lyophilized NCL-CD151 P (HIER) 1 mL lyophilized NCL-PC P (Enzyme) See also CD151 (PETA-3) on page 87. The plasma cell is the resultant terminal stage of B cell differentiation and apart from morphological features may be distinguished from other B cells by their lack of surface HLA class I and class II antigens, surface immunoglobulin, Fc and C3 receptors or presence of intracytoplasmic immunoglobulin. There have been some interesting studies where non- P-glycoprotein (CD243) Hodgkin’s lymphomas have been differentiated from plasmacytomas and myelomas based on plasma cell markers (Seegmiller et al., American Clone 5B12 Journal of Clinical Pathology. 127(2):172-174 (2007)). 1 mL lyophilized NCL-PGLYm F P (HIER) The resistance of tumor cells to cytotoxic chemotherapeutic drugs is a major problem in the treatment of cancer. Studies have linked the presence of a 170 to 180 kD cell membrane protein, P-glycoprotein, with resistance to a wide range of lipophilic chemotherapeutic drugs, a phenomenon known as multidrug resistance. P-glycoprotein is reported to be expressed in tran- sporting epithelia of several normal tissues, including liver, kidney, colon, adrenal and brain.
Human tonsil: immunohistochemical staining for plasma cells using NCL-PC. Note cytoplasmic Placental Alkaline Phosphatase staining of plasma cells. Paraffin section. Clone 8A9 1 mL, 0.1 mL lyophilized NCL-PLAP-8A9 F P (HIER) Primary Antibodies 1 mL liquid NCL-L-PLAP-8A9 F P (HIER) Plasminogen Activator Inhibitor (Type 1) 7 mL ready-to-use RTU-PLAP-8A9 F P (HIER) 7 mL Bond ready-to-use PA0161 P (HIER) Clone TJA6 Placental alkaline phosphatase (PLAP) is a membrane-associated 1 mL, 0.1 mL lyophilized NCL-PAI-1 P sialoglycoprotein enzyme normally present at high concentration in sync- Plasminogen activator inhibitor (Type 1, PAI-1) is a 48 kD protein which ytiotrophoblasts within the placenta during the third trimester of gestation. inhibits the conversion of plasminogen to plasmin. It is the principal inhibitor The expression of PLAP was originally thought to be restricted to term of the plasminogen activators t-PA and u-PA. PAI-1 is structurally related to placenta but a human PLAP-like variant has been described which shares the serine protease inhibitor (serpin) superfamily. The serpins are known to more than 85 percent homology with PLAP itself. This high degree of undergo a conformational rearrangement upon cleavage of the reactive homology between PLAP and PLAP-like enzyme together with cross- central peptide bond (P1-P1') and it is this conformational difference reacting antibodies has led to some confusion of the distribution of PLAP between the active and cleaved forms which determine their reactivity. The and PLAP-like enzyme in various tissues. PLAP is reported to be expressed PAI-1 protein is reported to be overexpressed in a number of malignancies only in normal term placenta, endocervix and fallopian tube and also in including ovarian cancers and derived cell lines, endometrial cancers, ovarian and proximal gastrointestinal tumors. PLAP expression is rare in cervical cancer, malignant brain cancers, nasopharyngeal carcinomas, malignant germ cell tumors. PLAP-like enzyme is reported to be breast tumors, head and neck squamous cell carcinomas and gastric predominantly found in normal fetal and neonatal testis, and in thymus. It is cancers. PAI-1 is also reported to be expressed by endothelial cells and is also commonly expressed in germ cell tumors and more recently described stored in platelets. in seminomas. Product Specific Information Reports indicate that clone 8A9 stains seminomas and placenta indicating a specificity for both PLAP and PLAP-like enzyme. Refer to page 39 for the Bond ready-to-use format.
Human ovarian adenocarcinoma: immunohistochemical staining for plasminogen activator inhibitor type 1 using NCL-PAI-1. Note intense cytoplasmic staining of malignant tumor cells. Paraffin section.
Human seminoma: immunohistochemical staining for placental alkaline phosphatase using NCL-PLAP-8A9. Note intense membrane staining of tumor cells. Paraffin section.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. erto.Ln ,Srpoocspemna utr-oiiefudfo nifce joint. infected an from fluid culture-positive pneumoniae Streptococcus G, Lane nasopharyngeal F, secretion. PCR-positive Lane pneumolysin samples. but sputum culture-negative culture-positive weight pneumoniae pneumoniae molecular Streptococcus Streptococcus A, E, Lane to NCL-SPNm. B using Lanes kD) markers. (53 pneumolysin of detection blot: Western to pneumonia and sinusitis from disease, human meningitis. of and range septicaemia important which wide all organism a by an (pneumococcus), produced causes pneumoniae protein Streptococcus species-specific of kD strains 53 a is Pneumolysin NCL-SPNm lyophilized mL 1 9.1/2/3/6 Clone Pneumolysin NCL-PDEGF. with immunoblotted tonsil human B, Lane markers. NCL-PDEGF. weight using kD) molecular (53 A, factor Lane growth endothelial platelet-derived of detection blot: Western and macrophages muscle, homeostasis. in thymidine smooth expression body total high for epithelium, important The be testis. may gastro-intestinal skin being and lung in No expression glands, (TP). epithelia. highest reported some adrenal and the is cells phosphorylase be glial cytoplasm, to cells, expression and stromal thymidine reported macrophages, during nucleus is in described transiently PDEGF be the of only healing. in number observed wound to expressed is and a growth and development shown by regulated endothelial reproduction, controlled tightly platelet-derived also is is is Angiogenesis It (PDEGF) which process. of factor multi-step one factors, complex vascular existing angiogenic a an from is vessels blood bed new of formation the Angiogenesis, NCL-PDEGF lyophilized mL 0.1 mL, 1 P-GF.44C Clone Factor Growth Endothelial Platelet-Derived W HE)W (HIER) P coiu 9 osci 4vrs oivrstps1 n n Influenza and 3 and in 2 1, effective B. types and A Poliovirus is types virus, virus, virus Measles B4 virus, virus, Mumps Coxsackie 2, 19, and BK 1 echovirus types Varicella- virus Adenovirus, Respiratory simplex and 4b, Herpes and with virus, 3 zoster 2, virus 1, cross-react types virus JC Parainfluenza not virus, syncytial does both NCL-JCBK for immunocytochemistry. specific productive NCL-JCBK, human in lytic brain tissue. Information a or post-mortem Specific or urine Product biopsy undergoes infected in following directly from detected virus or cultured or cells be the glial fetal may infection which virus is HIV virus JC in JC infection. of in cell oligodendrocyte target also The the transplantation. and organ for leukemia pro- or immunosuppression disease disease, lymphocytic of Hodgkin's complication as lethal subacute a such chronic as conditions rare mainly a malignant seen a is disseminated cause which advanced is CNS is may the PML of virus virus disease (PML). demyelinating JC JC BK leukoencephalopathy Unlike multifocal cancer. Polyomavirus, immunosuppression. gressive a human or also any cause without pregnancy urine virus, they the during in that shed symptoms occasionally be apparent evidence may and no humans vitro in in ubiquitous is cells the mammalian there viruses transform from BK and and but isolated hamsters JC newborn first condition. in demyelinating was oncogenic rare a are Papovaviridae with the individual an of of brain Polyomavirus a virus, JC viruses) (JC/BK NCL-JCBK Polyomavirus lyophilized mL 0.1 mL, 1 3.1.1 Clone Polyomavirus C-Rp oectpamcsann ftmrcls aafnsection. Paraffin cells. tumor of using staining prealbumin cytoplasmic for Note staining NCL-PREp. immunohistochemical tumor: carcinoid cell small Human including amyloidosis, systemic of II. forms and I subunit types hereditary polyneuropathy fibril amyloid major several familial the as in identified and been hormones protein has thyroid for prealbumin in protein Variant synthesized transport A. a molecule vitamin as kD serves 55 Prealbumin a liver. is the transthyretin, as known also Prealbumin, NCL-PREp lyophilized mL 1 Polyclonal Prealbumin W P F etr blotting Western Paraffin Frozen IO C I lwcytometry Flow Immunofluorescence (Enzyme) P F O E te applications Other lcrnmicroscopy Electron 151 /
Primary Antibodies Progesterone Receptor Progesterone and Estrogen Receptor
Clone 16 Antibodies (duo packs) 2 mL lyophilized NCL-PGR-312/2 P (HIER) W Clone 6F11, Clone 1A6 1 mL, 0.1 mL lyophilized NCL-PGR-312 P (HIER) W 2×1mLlyophilized NCL-ER/PGRd/1 F P (HIER) W 2 mL liquid NCL-L-PGR-312/2 P (HIER) W 2 × 0.5 mL lyophilized NCL-ER/PGRd F P (HIER) W 1 mL liquid NCL-L-PGR-312 P (HIER) W Reference Range 7 mL ready-to-use RTU-PGR-312 P (HIER) Clone 6F11, Clone 16 7 mL Bond ready-to-use PA0312 P (HIER) 2×1mLlyophilized NCL-ER/PGR-312d/1 F P (HIER) W 2 × 0.5 mL lyophilized NCL-ER/PGR-312d F P (HIER) W Clone 1A6 See also Progesterone and Estrogen Receptor Antibodies (duo packs) on 2 mL lyophilized NCL-PGR/2 P (HIER) W page 152. 1 mL, 0.1 mL lyophilized NCL-PGR P (HIER) W 1 mL liquid NCL-L-PGR P (HIER) W 7 mL ready-to-use RTU-PGR P (HIER) Clone 16 was developed to produce superior staining on paraffin sections. Proinsulin The human progesterone receptor (PR) is expressed as two isoforms, PRA (94 kD) and PRB (114 kD), which function as ligand-activated transcription Clone 1G4 factors. These two isoforms are transcribed from distinct estrogen receptor 1 mL lyophilized NCL-PROIN-1G4 P (HIER) (ER)-inducible promoters within a single copy PR gene. The PRA form is a Preproinsulin is converted to proinsulin by the action of a signal peptidase in truncated version of the PRB form, lacking the first 164 N-terminal amino the lumen of the endoplasmic reticulum within pancreatic beta cells. The acids. In humans, PRA acts as a transdominant repressor of the proinsulin is then transported from the endoplasmic reticulum to the Golgi transcriptional activity of PRB, glucocorticoid receptor, ER, androgen apparatus and is further modified by the action of various enzymes to yield receptor and mineralocorticoid receptor. PRB functions mainly as a the mature hormone, insulin. Insulinomas exhibit many structural features in transcriptional activator. PRB is expressed strongly in endometrial glandular common with normal beta cells. Studies of proinsulin and insulin have and stromal nuclei in the proliferative phase of the menstrual cycle and reported proinsulin/insulin expression patterns to vary greatly among those Primary Antibodies weakly during the secretory phase and early pregnancy. tumors and no correlation seems to exist between the expression staining Product Specific Information patterns and a particular histological tumor type. A diffuse expression pattern may be observed for proinsulin which differs from the crescent- Clone 1A6 reacts with a homologous region to both the A and B forms of PR. shaped perinuclear staining seen in normal beta cells suggesting Clone 16 is specific for a region of the N-terminus of the A form of PR. The abnormalities in the prohormone processing. This may be observed in about precise epitope has not been mapped but it reacts with both A and B forms of 50 percent of insulinomas. PR by Western blot but only with the A form by immunohistochemistry. This suggests that the epitope is inaccessible in the native folded B form of the protein. Refer to page 39 for the Bond ready-to-use format. Proliferating Cell Nuclear Antigen
Clone PC10 1 mL, 0.1 mL lyophilized NCL-PCNA PWC 1 mL liquid NCL-L-PCNA PWC Proliferating cell nuclear antigen (PCNA) is a 36 kD protein which is highly conserved between species. PCNA functions as a co-factor for DNA polymerase delta in S phase and also during DNA synthesis associated with DNA damage repair mechanisms. The PCNA molecule has a half-life in excess of 20 hours, and therefore, may be detected in non-cycling cells eg those in G0 phase. Human breast carcinoma (serial sections): immunohistochemical staining for progesterone Product Specific Information receptor using NCL-PGR (A) and NCL-PGR-312 (B). Note good nuclear staining in A but more intense nuclear staining of tumor cell nuclei in B. Paraffin sections. Heat induced epitope retrieval using 10mM citrate buffer (pH6.0) may improve staining on overfixed tissues, but due to increased sensitivity using this technique, care must be taken with the interpretation of results. Staining is reduced (and may be abolished) if sections are baked onto glass slides. Air drying overnight onto 3-aminopropyltriethoxysilane (Apes) coated slides may produce improved results.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. C-S-3.Nt nes yolsi tiigo lnua pteilcls aafnsection. Paraffin cells. epithelial glandular of staining using cytoplasmic antigen intense specific Note prostate NCL-PSA-431. for staining immunohistochemical gland: prostate Human format. breast ready-to-use Bond as the for such 39 page tumors to Refer and is PSA tissues PSA of non-prostatic gland. expression in of prostate carcinomas. levels reported the Low been and from phosphatase. identical have from acid isolated immunologically prostatic purified protein be from a and to distinct to isolated found similar originally was It biologically was kallikrein plasma. and the seminal to proteases belonging human protein serine kD of 34 a family is (PSA) antigen specific sections. Prostate paraffin on staining superior produce to developed was 35H9 Clone RTU-PSA-28A4 ready-to-use mL 7 NCL-L-PSA-28A4 liquid mL 1 28/A4 PSA Clone PA0431 ready-to-use Bond mL 7 NCL-PSA-431 lyophilized mL 0.1 mL, 1 35H9 Clone Antigen Specific Prostate section. Paraffin cells. tumor proliferating of using staining antigen nuclear nuclear Note cell NCL-PCNA. proliferating for staining immunohistochemical seminoma: Human FP (HIER) P FP P Reference Range C--SA oectpamcsann ftmrcls aafnsection. Paraffin cells. tumor using of PSMA staining for cytoplasmic staining Note immunohistochemical NCL-L-PSMA. liver: in carcinoma prostate metastatic Human proteins. secreted are that (PAP) antigen phosphatase specific acid prostate The prostatic of involvement. and that metastatic (PSA) with of contrasts correlate PSMA indicator of an may localization as cellular serve expression a and as PSMA burden other tumor that the with suggest and as one hydrolase Reports activities, folate enzymatic carboxypeptidase. 750 membrane two protein integral a has the PSMA of prostate-specific as form acids. a a amino expressed PSM, 57 as first is found the be missing (PSMA) also may antigen but glycoprotein acid membrane amino specific prostate The NCL-L-PSMA liquid mL 1 1D6 Clone Antigen Membrane Specific Prostate rti sn C-TV.Nt ebaesann fpottcnolsi el.Paraffin cells. neoplastic prostatic of staining section. membrane Note NCL-PTOV1. overexpressed using tumor 1 prostate protein for staining immunohistochemical carcinoma: prostatic Human PTOV1 1.8kb with a prostate. cytoplasm normal of in muscle, the levels skeletal lower detection heart, at in brain, and The human liver and normal mainly kidney cells. in reported located been tumor has is prostatic transcript in advanced-stage protein is positivity in protein PTOV1 juxtanuclear and PTOV1 cells. those neoplasia as addition, prostatic cancer such intra-epithelial In biopsies benign prostate prostatic tissue. in from with cells prostate than premalignant normal cancers in overexpressed in prostate as or in at arise expressed the hyperplasia levels be called to cells higher reported tissue, is carcinomatous PTOV1 hyperplastic significantly hyperplasia. of peripheral cancer, benign background of the prostate a zone In against of of cells. lesions and invasive conversion form multifocal malignant, genetic phenotypic into common to of the cells most susceptible prostate number for was hyperplastic highly A responsible or PTOV1, is normal are changes. males or alterations proliferative in ageing molecular malignant progression 1, in of and prostate protein markers benign The molecular overexpressed cancer. for search prostate tumor the following prostate discovered gene The NCL-PTOV1 lyophilized mL 1 17C6 Clone 1 Protein Overexpressed Tumor Prostate W P F etr blotting Western Paraffin Frozen C I (HIER) P lwcytometry Flow Immunofluorescence (HIER) P O E te applications Other lcrnmicroscopy Electron 153 /
Primary Antibodies Prostatic Acid Phosphatase Protein Gene Product 9.5
Clone PASE/4LJ Clone 10A1 1 mL liquid NCL-L-PAP FP 1 mL, 0.1 mL lyophilized NCL-PGP9.5 F P (HIER) W 7 mL Bond ready-to-use PA0006 P (HIER) 1 mL liquid NCL-L-PGP9.5 F P (HIER) W Prostatic acid phosphatase (PAP) is an isoenzyme of acid phosphatase 7 mL Bond ready-to-use PA0286 P (HIER) found in large amounts in the prostate and seminal fluid. The precise Protein gene product (PGP) 9.5 is a neuron specific protein, structurally and function of PAP is unknown, but it may act as a hydrolase to split phosphoryl immunologically distinct from neuron specific enolase. The protein which choline in semen and also function as a transferase. Elevated serum levels has a molecular weight of 27 kD was first identified by high resolution two of the enzyme are reported in metastatic prostatic carcinoma. dimensional PAGE. PGP9.5 expression has been reported in neurons and Refer to page 40 for the Bond ready-to-use format. nerve fibers at all levels of the central and peripheral nervous system, in many neuroendocrine cells, in segments of the renal tubules, in spermatogonia and Leydig cells of the testis, in ova and in some cells of both the pregnant and non-pregnant corpus luteum. PGP9.5 is known to be a member of the ubiquitin C-terminal hydroxylase family and is also concentrated within inclusion bodies suggesting that such structures may be metabolically active regions of the cells. Refer to page 40 for the Bond ready-to-use format.
pS2 Protein Prostate adenocarcinoma: immunohistochemical staining with Prostatic Acid Phosphatase (PASE/4LJ) using Bond Polymer Refine Detection. Polyclonal 0.5 mL lyophilized NCL-pS2 FP pS2, also known as pNR-2, was first identified by differential screening of
Primary Antibodies cDNA libraries from estrogen responsive breast cancer cell lines. In normal Prostatic Inhibin Peptide tissue, pS2 protein is reported to be expressed in gastric mucosa, small intestinal mucosa and normal breast epithelium. pS2 is estrogen regulated in Clone 4A6A6 breast cancer cell lines and may have some growth factor activity. In 1 mL lyophilized NCL-PIP P malignant epithelial tumors, pS2 has been reported to be expressed in gastric carcinomas and gynecological cancers. The pS2 mRNA and protein Prostatic inhibin peptide (PIP), a 10.7 kD follicle stimulating hormone are expressed predominantly in estrogen receptor positive breast cancers. suppressing molecule, is synthesized and secreted by the prostate gland. PIP has been reported to be expressed in primary prostatic tumors and their metastases. PIP has also been reported to be expressed in the serum and urine of patients with prostatic cancer. PIP expression is unaffected by reduced or absent androgens unlike both PSA and PAP whose expression is dependent on androgens.
Human breast carcinoma: immunohistochemical staining for pS2 protein using NCL-pS2. Note cytoplasmic staining of a proportion of tumor cells. Paraffin section.
Human prostate gland: immunohistochemical staining for prostatic inhibin peptide using NCL-PIP. Note cytoplasmic staining of glandular epithelium. Paraffin section.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. oencersann napooto fmlgatcls aafnsection. Paraffin cells. malignant of proportion a in NCL-PTEN. staining using nuclear PTEN Note for staining immunohistochemical melanoma: malignant Human breast both in thyroid occur in to and reported found breast is cancer. for expression are prostate gene and predisposition PTEN PTEN a of of to Loss cancers. leads mutations which Germline syndrome and Cowden carcinoma cancer. lung melanoma, ovarian cell malignant small cancer, endometrial carcinoma, breast bladder including PTEN cancers carcinoma, to of endometrial mutations range In and a dephosphorylating Deletions in by recognition. occur 5-triphosphate. FAK apoptosis 4, and to reducing 3, resistance (FAK) spreading phosphatidylinositol prevent kinase may adhesion, adhesion. adhesion PTEN cell cell addition, focal possibly the affecting and with apoptosis phosphorylation interacts cycle, which cell also gene ubiquit- the PTEN suppressor almost regulates tumor expressed and a is which is ously phosphatase TEP1, multifunctional or a MMAC1 encodes as known also PTEN, NCL-PTEN lyophilized mL 0.1 mL, 1 28H6 Clone PTEN section. Paraffin platelets. of staining intense antigen Note CD62P NCL-CD62P-367. for using staining (P-selectin) immunohistochemical vessel: blood cord, umbilical Human of activated adhesion integrin-mediated to the inhibits endothelium. to antigen neutrophils neutrophils Sialyl- CD62P activated soluble structures of A carbohydrate the cells. the attachment mediates to is rolling binds and P-selectin, Lewis antigen monocytes as the CD62P and known endothelium. neutrophils for with also platelets kD), responsible activated (140 of antigen interaction CD62P The NCL-CD62P-367 lyophilized mL 1 C34 Clone (CD62P) P-selectin x nnurpisadt aatslcrmdso etohl n tumor and neutrophils on ceramides galactosyl to and neutrophils on (HIER) P (HIER) P C-C sseii o rxmlnprgncrnlatgncst nthe on site antigenic renal nephrogenic gp200. of proximal domain a carbohydrate for specific is NCL-RCC carcinomas. cell Information approximately renal Specific metastatic Product in of percent expressed 84 be and primary to of luminal thyroid antigen percent of the 93 gp200 colloid ducts, the indicate the be within and Reports and to lobules adjoining epithelium follicles. tubular reported breast capsule epididymal of also the of surface Bowman's is surface luminal gp200, of the glycoprotein, on surface brush The expressed renal the luminal tubule. proximal within the the proximal localized of outgoing on segments is recta and glycoprotein pars and kD tubule convoluta 200 pars the a of kidney, border normal the In NCL-RCC lyophilized mL 0.1 mL, 1 66.4.C2 Clone Marker Carcinoma Cell Renal ese iu,IfunavrstpsAadB oivrstps1 n 3, and 2 1, virus, 2. and types Mumps 1 Poliovirus types Adenovirus, virus B, and simplex Cytomegalovirus 4b, Herpes and virus, Varicella-zoster and 19, A echovirus virus, 3 types B4 Coxsackie virus 2, cross-react Influenza not 1, virus, does types NCL-RSV3 Measles virus antibodies. four Parainfluenza of with cocktail a is NCL-RSV3 Information Specific Product cocirculate RSV lower of subtypes significant and whom population. types the in Multiple with in high. individuals be or associated immunosuppressed may rates and weight con- also mortality elderly with birth in is disease those low virus respiratory particularly dysplasia, The RSV die, bronchopulmonary an months immunodeficiency. defects, develop six who heart few and babies first genital of two the percent during between one pneumonia infection of Approximately of cases cases life. all all of of of months percent percent 50 25 approximately and for bronchiolitis pathogen responsible respiratory is important and most childhood the of is (RSV) virus syncytial Respiratory G12 5H5N, Clone virus syncytial Respiratory Mouza Dr of courtesy supplied Al-Sharhan. Section A. section. Abdulla Paraffin Note cells. NCL-RCC. tumor using of gp200 staining for cytoplasmic staining immunohistochemical carcinoma: cell renal Human LlohlzdNCL-RSV3 lyophilized mL 1 W P F etr blotting Western Paraffin Frozen 2 A,I3cocktail IC3 5A6, , C I lwcytometry Flow Immunofluorescence HE)I (HIER) P F (Enzyme) P O E Reference Range te applications Other lcrnmicroscopy Electron 155 /
Primary Antibodies ret Oncoprotein
Clone 3F8 1 mL, 0.1 mL lyophilized NCL-RET P (HIER) The ret proto-oncogene encodes a cell surface glycoprotein belonging to a member of the receptor tyrosine kinase family and is located on chromosome 10q11.2. Three main 3' splice isoforms have been characte- rized from papillary thyroid carcinomas, themselves originating from thyroid epithelial cells. ret expression is reported in several regions of the central nervous system; in the developing cranial nerve ganglia and a subset of Human infant lung, post-mortem tissue: immunohistochemical staining for Respiratory cells within dorsal root ganglia, in motor neurons in the spinal cord and syncytial virus (RSV) using NCL-RSV3. Note intense staining of infected luminal bronchial hindbrain, in neuroretina and the growing tips of the renal collecting ducts in epithelial cells. Paraffin section. developing kidney. Some individuals with Hirschsprung's disease have severe developmental abnormalities of the kidney and these phenotypic abnormalities may be linked with mutations of ret proto-oncogene. About 70 percent of individuals who carry one of the documented ret mutations that predispose to multiple endocrine neoplasia type II (MENII) will develop Retinoblastoma Gene Protein thyroid C cell derived tumors in their lifetime. Clone 13A10 Product Specific Information NCL-RET was raised to the intracellular domain of the molecule, present in all 1 mL, 0.1 mL lyophilized NCL-RB-358 F P (HIER) W isoforms of the protein. Mutations are reported to occur upstream of this 1 mL liquid NCL-L-RB-358 F P (HIER) W domain. Clone 1F8 1 mL lyophilized NCL-RB F P (HIER) W Clone 13A10 was developed to produce superior staining on paraffin sections.
Primary Antibodies Retinoblastoma (Rb) is a rare tumor of the retina associated with mutations of chromosome 13. The nuclear phosphoprotein encoded by the Rb tumor suppressor gene is present in many cells and may indirectly regulate cell growth by activating the transcription factor ATF-2. Activation of ATF-2 initiates expression of TGF-beta2, which in turn inhibits transcription of genes affecting cell growth. Bilateral mutation of the Rb gene may potentially play a role in the development of a number of malignant tumors. Human small intestine: immunohistochemical staining for ret oncoprotein using NCL-RET. Product Specific Information Note cytoplasmic staining of enteric ganglion cells. Paraffin section. NCL-RB-358 was raised to the N-terminal region of the Rb gene protein. RHAMM (CD168)
Clone 2D6 1 mL lyophilized NCL-CD168 F P (HIER) Refer to page 88 for further information about CD168.
Human tonsil: immunohistochemical staining for retinoblastoma gene protein using NCL-RB- 358. Note intense nuclear staining of epithelial cells. Paraffin section.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. oeitnesann ftmrcls aafnsection. Paraffin cells. tumor of NCL-L-S100A7. staining using intense S100A7 Note for staining immunohistochemical melanoma: malignant Human cell and squamous estrogen in also with keratinization is abnormal associated It in metastasis. are expressed nodal carcinoma. be S100A7 and to status of reported negative breast receptor and levels in be that progesterone intracellular to indicate high reported Reports of entirely, epithelium. cancer, variety not squamous of although stratified family a to largely, gene confined is regulate S-100 It the which processes. of member extracellular proteins a binding is psoriasin, calcium as known also S100A7, NCL-L-S100-A7 liquid mL 1 3DC Clone (Psoriasin) S100A7 format. ready-to-use Bond the for 40 cases. page some to in Refer (HIER) retrieval rat. epitope and induced mouse heat with horse, require monkey, reactive may cat, also NCL-S100 dog, are pig, RTU-S100p chicken, and cow, and cases from for protein some NCL-L-S100p S-100 recommended in not NCL-S100p, pretreatment is enzyme elements. so require and neural may S-100 of of form staining during A the the tissue for specific frozen is NCL-S100 from eluted Information be Specific Product may and lymph S-100 soluble that of procedures. noteworthy immunohistochemical highly paracortex is It is the is protein. in S-100 S-100B express protein cells to Langerhans reported reticulum whereas also interdigitating melanocytes. are in chain nodes and expressed and skin be beta to nerves in reported and cells including is protein alpha tissue, S-100 chains. an neuroectodermal beta of two of proteins. of composed composed family S-100 is the of members S-100A two are proteins S-100B and S-100A PA0900 ready-to-use Bond mL 7 RTU-S100p ready-to-use mL 7 NCL-L-S100p polyclonal liquid mL 1 NCL-S100p lyophilized mL 1 Polyclonal NCL-S100 lyophilized mL 0.1 mL, 1 S1/61/69 Clone S-100 (HIER) P FP FP (Enzyme) P FP FP Reference Range h hl ru,btterdcinmyb otsvr o h mutated the for severe membrane most monitor to be necessary may for is integrity. beta-spectrin expression reduction for reduced Labeling the show protein. but single usually group, genes sarcoglycan whole the the of mutations proteins one with individuals any complex subcomplex, in a of as the together subgroup As function sarcoglycan. sarcoglycans gamma sarcoglycan and (adhalin) the alpha-sarcoglycan includes which in with expression show individuals (SCARMD) dystrophy many defective muscular Also, recessive gene. autosomal childhood delta-sarcoglycan severe LGM2F the with those in and mutations with gene, those have beta-sarcoglycan individuals the alpha-sarcoglycan, in for example, mutations gene have For the and LGM2E in review. complex mutations is under have picture LGMD2D currently with the is dystrophy: that muscular classification shown of disease are have types complex several glycoprotein studies dystrophin in the a (dystrophin Recent altered of proteins members labeling. shows different seven DAG of muscle expression least in Dystrophin-deficient at reduction of DAGs). generalized complex glycoproteins, the a to associated attached via is dystrophy, membrane muscular which Becker gene muscle and the Duchenne of in product protein defective the is dystrophin, muscle, skeletal normal In gamma NCL-g-SARC sarcoglycan, lyophilized mL 0.1 mL, 1 35DAG/21B5 Clone NCL-d-SARC delta sarcoglycan, lyophilized mL 1 Clone NCL-L-b-SARC beta sarcoglycan, liquid mL 1 beta NCL-b-SARC sarcoglycan, lyophilized mL 0.1 mL, 1 Clone NCL-L-a-SARC (adhalin) alpha sarcoglycan, liquid mL 1 (adhalin) alpha NCL-a-SARC sarcoglycan, lyophilized mL 0.1 mL, 1 Ad1/20A6 Clone Antibodies Sarcoglycan egtmres aeB ua uceetatimnbotdwt NCL-L-a-SARC. with immunoblotted extract molecular muscle A, human Lane B, NCL-L-a-SARC. Lane using markers. kD) weight (50 alpha-sarcoglycan of detection blot: Western W P F etr blotting Western Paraffin Frozen Sarc3/12C1 Sarc1/5B1 FE FW FE FWE FWE C I lwcytometry Flow Immunofluorescence O E FE te applications Other lcrnmicroscopy Electron 157 /
Primary Antibodies Sarcoplasmic or Endoplasmic Reticulum Sialyl Lewisa (CA19-9) 2+ Ca ATPase (SERCA) Antibodies Clone C241:5:1:4 1 mL lyophilized NCL-CA19-9 F P (HIER) Clone IID8 1 mL liquid NCL-L-CA19-9 F P (HIER) 0.5 mL lyophilized NCL-SERCA2 FP Refer to page 64 for further information about CA19-9. ATP-dependent calcium pumps are responsible in part for the maintenance of low cytoplasmic free Ca2+ ion concentrations. The ATP pumps that are located in intracellular organelles are encoded by a family of structurally related enzymes termed the sarcoplasmic or endoplasmic reticulum Ca2+ ATPases (SERCA). The SERCA1 gene is exclusively expressed in type II SM22 alpha (fast) skeletal muscle. The SERCA2 gene is subject to tissue dependent processing which is responsible for the generation of two specific isoforms. SERCA2a muscle-specific isoform is reported to be expressed in type I Clone 10H12 (slow) skeletal, cardiac and smooth muscle whilst the SERCA2b isoform is 1 mL liquid NCL-L-SM22a P (HIER) W reported to be expressed in all cell types. The SERCA3 gene is less well SM22 alpha, also known as smooth muscle cell specific protein, is reported characterized and is found in non-muscle cells. to be expressed at high levels in various tissues such as intestine, lung and uterus. It shares structural homology with the regulatory protein, calponin. SM22 alpha expression has been reported in most tissues that contain smooth muscle in both vascular and visceral forms. It is also reported to be expressed in some rare malignant tumors such as malignant fibrous histiocytomas.
Primary Antibodies Human skeletal muscle: immunohistochemical staining for sarcoplasmic or endoplasmic reticulum Ca2+ ATPase 2 using NCL-SERCA2. Note the staining of type I myofibers in muscle cells. Paraffin section.
Human prostate: immunohistochemical staining for SM22 alpha using NCL-L-SM22a. Serotonin Note intense staining of smooth muscle. Paraffin section. Polyclonal 0.5 mL lyophilized NCL-SEROTp P 7 mL Bond ready-to-use PA0736 P (HIER) SMA (Alpha Smooth Muscle Actin) Serotonin (5-hydroxytryptamine, 5-HT) is reported to be a widely distributed neurotransmitter and hormone in the mammalian peripheral and central Clone sm-1 nervous system (CNS). Serotonin is formed by the decarboxylation of 5- 1 mL lyophilized NCL-SMA F P (Enzyme) W hydroxy-tryptophan, its intermediate, which in turn is formed by 7 mL ready-to-use RTU-SMA F P (Enzyme) hydroxylation of L-tryptophan by tryptophan hydroxylase. In the CNS, the 7 mL Bond ready-to-use PA0943 P action of serotonin is terminated by reuptake into the presynaptic terminal by specific serotonin transporters. Serotonin has been implicated in several Refer to page 57 for further information about Alpha Smooth Muscle Actin. neuropsychiatric disorders such as anxiety, depression and schizophrenia. The majority of serotonergic nerve terminals in the CNS originate in neuronal cell bodies of the Raphé nuclei (dorsal, median), nucleus Raphé obscurus and nucleus Raphé pallidus in the brainstem which project to specific areas of the brain and spinal cord. Serotonin is thought to be an inhibitory neurotransmitter regulating a wide range of sensory, motor and cortical functions in the CNS. In the periphery, serotonin is reported to be present in neural and non-neural structures such as platelets, gastro- intestinal tract (myenteric plexus, enterochromaffin cells), lungs (neuroepithelial cells), thyroid gland and spleen. Refer to page 40 for the Bond ready-to-use format.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. hra C-PC ecsmdrtl ihhmnbt-pcrnand in beta-spectrin spectrin human beta-spectrin. beta-spectrin dog with and of human mouse rat, moderately with chain rabbit, with reacts weakly reacts beta NCL-SPEC2 NCL-SPEC1 the whereas muscle. recognize and NCL-SPEC2 erythrocytes and but NCL-SPEC1 dystrophin for Information Specific negative expression. Product dystrophin of are abnormalities true which reflect to spectrin fibers stages for necessary early positive the whereas negative in is (or show regeneration), damaged spectrin which are of spectrin fibers and for that dystrophin both reported Labeling for is labeling samples. It integrity. muscle membrane monitor excision of the muscular during freezing Becker occurs and and frequently Duchenne damage in membrane with defective Subtle homology is dystrophy. structural that some protein has the which dystrophin, protein cytoskeletal a is Spectrin NCL-SPEC2 spectrum) (broad Spectrin lyophilized mL 1 RBC1/5B1 Clone use) human NCL-SPEC1 for (recommended Spectrin lyophilized mL 1 RBC2/3D5 Clone Antibodies Spectrin section. Paraffin elements. neural of staining NCL-SNAP-25. cytoplasmic using intense SNAP-25 Note for staining immunohistochemical bowel: small Human the of layers molecular and layer cell hormone granule growth prolactinomas, the cerebellum. of been and has cells palmitoylated tumors SNAP-25 tumor by molecule. in secreting the expressed membrane a of be the region to lacks central reported to SNAP-25 the linked exocytosis. in residues is regulated a cysteine for but is complex make domain, to (SNAP-25) docking thought transmembrane are initial which of kD proteins an four consisting 25 of up one is complex of and protein a protein plasmalemmal plasma synaptic by Synaptosomal-associated the and vesicle mediated membrane. synaptic the both is of components of Exocytosis membrane exocytosis by vesicles. regulated is neurons synaptic from neurotransmitters of release The NCL-SNAP-25 lyophilized mL 0.5 SP12 Clone SNAP-25 FWE HE)W (HIER) P F naclrtdaen ftebanadhsoahlgclcagsare changes histopathological and Down's brain in the develop and individuals where of These 21 effect ageing identified. chromosome been dosage accelerated have on gene mRNA an a SOD1 carried of reflects is levels activity with high SOD1 SOD1 in individuals for increased cells, granule syndrome, and gene and pyramidal The humans neurons, level hippocampal AD. higher of normal a subsets indicated in Reports from SOD1 studied. of been enzyme hippocampi has SOD (AD) dismutation disease copper/zinc the the Alzheimer's the in containing in acceptor cells electron (SOD1) of an Cu distribution one as The and a involved Zn reaction. being is one containing latter enzyme subunit the cytoplasmic each radicals atom, The with subunits SOD. identical superoxide cytoplasmic of cells dimer and by eukaryotic mitochondrial all Almost defence a caused metabolism. cellular aerobic have of a damage by-product as a act as oxidative produced to necessary is against a function is Their mechanism which activity. are ion enzymic enzymes metal for These the cofactor peroxide. to hydrogen according and dismutation classified oxygen the metalloproteins to catalyzes anion which superoxide enzyme an of is (SOD) dismutase Superoxide NCL-SOD1 lyophilized mL 1 30F11 Clone (Cu/Zn) Dismutase Superoxide with immunoblotted muscle human A, of Lane extract NCL-SPEC1. NCL-SPEC1. urea using B, muscle) Lane in markers. kD weight (253 molecular beta-spectrin human of detection blot: Western eladlrecl acnmso h ugadnnumnr adeno- nonpulmonary and lung the pulmonary SP-B. of or pro-SP-B of express carcinomas to not percent cell reported are carcinomas large 53 Squamous and and patterns. growth cell solid percent and bronchoalveolar adenocarcinomas papillary, 60 acinar, in with noted expression approximately with to respectively, reported mouse, in adenocarcinomas, been human, have adult mRNA found SP-B of and cells non-collagenous be protein epithelial Pro-SP-B lung. bronchiolar kD rabbit in and and in 9 rat detected cells been II a has type mRNA in both SP-B SP-B. kD resulting surfactant, 42 pulmonary of SP-D. processing hydrophobic weight and molecular proteolytic SP-C a alveolar with SP-B, undergoes (pro-SP-B) SP-A, providing B are protein the have precursor interface, surfactant Surfactant which pulmonary proteins reduces air-liquid from distinct isolated Four that alveolar been ventilation. normal mixture the for necessary phospholipid-rich at stability a tension is surface surfactant Pulmonary NCL-SPPB lyophilized mL 0.1 mL, 1 19H7 Clone B Protein Precursor Surfactant AD. of that to reminiscent W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence HE)W (HIER) P (HIER) P O E te applications Other lcrnmicroscopy Electron 159 /
Primary Antibodies Synaptic Vesicle Protein 2
Clone 15E11 1 mL lyophilized NCL-SV2 P (HIER) Synaptic vesicle protein 2 (SV2) is an integral membrane glycoprotein. It is required for normal neurotransmission and may play a role in the regulation of calcium-stimulated exocytosis. SV2 exists in three isoforms, SV2A, SV2B and SV2C, each containing 12 transmembrane spanning regions. SV2 proteins are reported to be among the most abundant and conserved components of synaptic vesicles in vertebrates. They are present on all Human lung adenocarcinoma: immunohistochemical staining for surfactant precursor protein small synaptic vesicles independent of transmitter type. SV2A and SV2B are B using NCL-SPPB. Note cytoplasmic staining of Clara cells and extracellular protein. Paraffin reported to be widely distributed in the nervous system, whereas SV2C is section. only observed in a small number of neurons in a few areas of the brain. In the brain, SV2A is reported to be expressed at the highest levels in subcortical regions, whereas the highest level of expression of SV2B is in the cortex and hippocampus. SV2 is also reported to be expressed on secretory vesicles of neuroendocrine cells in the gastrointestinal tract, Surfactant Protein A pancreas, anterior pituitary gland, thyroid, parathyroid and adrenal medulla and also in exocrine chief cells of gastric mucosa. Clone 32E12 Product Specific Information 1 mL, 0.1 mL lyophilized NCL-SP-A P (HIER) NCL-SV2 is raised to the N-terminal cytoplasmic region of the SV2A isoform. Pulmonary surfactant plays a critical role in maintaining the structural integrity of the respiratory epithelium by reducing surface tension during expiration. It is a lipoprotein complex which is synthesized and secreted into the alveoli of the lung by type II pneumocytes. Lung surfactant protein-A (SP-A) is a major phospholipid-associated glycoprotein in surfactant and is a member of the C-type lectin superfamily that also inhibits lipid secretion and enhances the uptake of phospholipid by alveolar type II cells. Levels of SP-A in amniotic fluid are reported to reflect the degree of fetal lung Primary Antibodies maturity and inadequate levels of surfactant at birth, a frequent occurrence in premature infants, results in respiratory failure. In individuals with lung adenocarcinomas, high concentrations of SP-A have been reported in pleural effusions except in poorly differentiated lung adenocarcinomas where a significant decrease of SP-A immunoreactivity has been reported Human cerebellum: immunohistochemical staining for synaptic vesicle protein 2 using (Zamecnik J and Kodek R. Virchows Arch. 440(4): 353-61 (2002). NCL-SV2. Note staining of neuronal processes. Paraffin section.
Synaptophysin
Clone 27G12 1 mL, 0.1 mL lyophilized NCL-SYNAP-299 F P (HIER) W 1 mL liquid NCL-L-SYNAP-299 F P (HIER) W 7 mL ready-to-use RTU-SYNAP-299 F P (HIER) 7 mL Bond ready-to-use PA0299 P (HIER) Human lung adenocarcinoma: immunohistochemical staining for surfactant protein A using NCL-SP-A. Note intense cytoplasmic staining of type II pneumocytes and alveolar Synaptophysin is an integral membrane glycoprotein with a molecular macrophages. Paraffin section. weight of 38 kD. It is reported to occur in presynaptic vesicles of neurons in brain, spinal cord, retina, in similar vesicles of the adrenal medulla as well as in neuromuscular junctions. Synaptophysin may be involved in synaptic vesicle formation and exocytosis. Synaptophysin is reported to be expressed in a wide spectrum of neuroendocrine tumors including neuro- blastomas, ganglioneuroblastomas, phaeochromocytomas, chromaffin and non-chromaffin paragangliomas. Synaptophysin is also reported to be expressed in neuroendocrine tumors of epithelial type including pituitary adenomas, islet cell tumors, medullary carcinomas of thyroid, parathyroid adenomas, carcinoids of the bronchopulmonary and gastrointestinal tracts, neuroendocrine carcinomas of the bronchopulmonary and gastrointestinal tract and neuronendocrine carcinomas of the skin. Refer to page 41 for the Bond ready-to-use format.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. sn C-RP oeitnectpamcsann fotolss aafnsection. Paraffin osteoclasts. of staining cytoplasmic intense Note phosphatase NCL-TRAP. acid using tartrate-resistant for staining immunohistochemical osteoclastoma: Human format. ready-to-use Bond and the for leukemias 41 page cell to Refer T macrophages and tissues. some embryonic cell and and maternal B throughout syncytiotrophoblasts distributed various cells, with and decidual associated spleen lymphomas, also are be the levels Elevated in to turnover. increased reported bone active the be undergoing and individuals disease to of Hodgkin's sera disease, reported 4-nitrophenyl and Gaucher's with is spleen individuals of osteoclasts, and TRAP monocytes macrophages, of alveolar ATP Expression human liver. in phosphoproteins, applications expressed different be through towards reported to been has isoenzyme activity protein This phosphate. iron-binding high basic, a is with (TRAP) phosphatase acid Tartrate-resistant PA0093 ready-to-use Bond mL 7 NCL-TRAP lyophilized mL 0.1 mL, 1 26E5 Clone (TRAP) Phosphatase Acid Tartrate-Resistant Alpha-Synuclein. about information further for 57 page to Refer NCL-L-ASYN liquid mL 1 KM51 Clone Alpha Synuclein, Paraffin cells. tumor of section. staining cytoplasmic Note NCL-SYNAP-299. for using staining synaptophysin immunohistochemical differentiation: neuroendocrine showing carcinoma Breast (HIER) P (HIER) P (HIER) P New! C-a-.Nt nes tiigo eiepausadtesronigdystrophic surrounding the section. and Paraffin plaques neurites. senile of using staining protein intense tau Note for NCL-Tau-2. staining immunohistochemical disease: Alzheimer's brain, Human the with cross-reacts protein, tau protein. tau bovine human of the form phosphorylated against raised NCL-Tau-2, major are whose Information filaments Specific disease Product helical protein. paired tau Alzheimer's microtubule-associated a distinct is component with ultrastructurally accumul- dense represent of threads Tangles individuals plaques. ations neuropil senile tangles, in neurofibrillary neurites from abnormal of and abundance tissues an by characterized brain The NCL-Tau-2 lyophilized mL 0.1 mL, 1 Tau-2 Clone Tau oeitrellrmti tiigaon ainn el.Prfi section. Paraffin cells. malignant around staining NCL-TENAS-C. matrix using intercellular tenascin Note for staining immunohistochemical carcinoma: breast Human NCL-TENAS-C. with staining optimal produces digestion 30 enzyme by of immediately followed seconds technique (HIER) retrieval epitope induced heat The Information Specific Product of recognition the ligand integrins. expression shares most for that by Like necessary The molecule migration. sequence adhesive and acid' osteogenesis. cell-substrate proliferation ‘arginine-glycine-aspartic a cell normal is with tenascin correlate for bone fibronectin, to of important reported cortex outer is and be tenascin periosteum of inner presence to the the on addition, appears fibers In growth III healing. the type wound organizing on in in as tenascin eg role known matrix a extracellular previously play the to was of thought itself is the Tenascin Tenascin and of TN-W4. antigen tenascin. members myotendinous and called TN-Y five originally unique TN-X, least was TN-R, a C at (TN-C), C are has tenascin growth There family, which epidermal fibrinogen. tenascin glycoprotein to and homologous fibronectin weight domains factor, molecular containing high structure molecular a is Tenascin NCL-TENAS-C lyophilized mL 1 49 Clone C Tenascin W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence (HIER+Enzyme) P F (HIER) P O E te applications Other lcrnmicroscopy Electron 161 /
Primary Antibodies Terminal Deoxynucleotidyl Transferase Thymus and Activation-Regulated
Clone SEN28 Chemokine 1 mL, 0.1 mL lyophilized NCL-TdT-339 P (HIER) W Clone 6SN 1 mL liquid NCL-L-TdT-339 P (HIER) W Reference Range 1 mL lyophilized NCL-TARC P (HIER) 7 mL ready-to-use RTU-TdT-339 P (HIER) 7 mL Bond ready-to-use PA0339 P (HIER) The attraction of leukocytes to sites of inflammation is a process controlled by chemokines. Chemokines are a group of proteins of 8 to 10 kD that are Terminal deoxynucleotidyl transferase (TdT) is a DNA polymerase of 58 kD divided into four families based on their first two cysteine residues in the located in the cell nucleus which catalyzes the polymerization of conserved motif. Thymus and activation-regulated chemokine (TARC) deoxynucleotides at the 3' hydroxyl ends of oligo or polydeoxynucleotide functions as a selective chemoattractant for T cells which express a class of initiators and functions without a template. TdT is reported to be expressed receptors binding TARC with high affinity and specificity. TARC has been in primitive T and B lymphocytes of the normal thymus and bone marrow. identified as the specific ligand for the CC chemokine receptor 4 (CCR4) The identification of TdT-positive cell populations in primary and secondary which is preferentially expressed at high levels in activated T helper 2 (Th2) lymphoid organs during maturation of the immune system is one area of cells. It is reported to be constitutively expressed in the dendritic cells of the interest but it is the reported occurrence of high levels of enzyme activity in thymus and transiently in activated peripheral blood mononuclear cells. It is white blood cells and bone marrow in certain leukemias which is of present in the cell cytoplasm with frequent paranuclear condensation. particular interest. Early and precise differentiation of lymphoblastic Reports indicate that TARC is overexpressed in nodular sclerosis (NS) and lymphoma is crucial. TdT is reported to be expressed in lymphoblastic mixed cellularity (MC) classical Hodgkin's disease but not in non-Hodgkin's lymphomas and leukemias. The determination of TdT expression is reported lymphomas, anaplastic large cell lymphomas and large B cell lymphomas to be most valuable when it is difficult to differentiate histologically between with CD30 positivity. Reed Sternberg cells of NS and MC Hodgkin's lymphoblastic lymphoma and Burkitt's lymphoma. lymphomas show high levels of TARC protein. Furthermore, in classical Refer to page 42 for the Bond ready-to-use format. Hodgkin's disease, TARC's ability to attract activated T cells by strong association to the chemokine receptor, CCR4, may explain the characteristic T cell infiltrate. It has also been suggested that TARC may also play a role in platelet activation, via CCR4, seen in Th2-associated diseases such as asthma and atopic dermatitis. Primary Antibodies
Human lymphoblastic lymphoma: immunohistochemical staining for terminal deoxynucleotidyl transferase using NCL-TdT-339. Note intense nuclear staining of tumor cells. Paraffin section.
Human cervical lymph node, Hodgkin's disease: immunohistochemical staining for thymus and activation-regulated chemokine (TARC) using NCL-TARC. Note characteristic staining of Reed Thrombomodulin (CD141) Sternberg cells. Paraffin section.
Clone 15C8 1 mL, 0.1 mL lyophilized NCL-CD141 F P (HIER) Thyroglobulin Thrombomodulin is a transmembrane glycoprotein of 75 kD which can accelerate the activation of protein C. Activated protein C functions as an anticoagulant by combining with protein S to inactivate factors Va and VIIIa Clone 1D4 of the blood coagulation pathway and by binding thrombin. Several factors 1 mL liquid NCL-L-THY FP regulate thrombomodulin expression. Downregulation of thrombomodulin 7 mL Bond ready-to-use PA0025 P may be induced by the cytokine interleukin-1, tumor necrosis factor and endotoxin. Agents which increase cyclic AMP such as forskolin may Thyroglobulin is a heavily glycosylated protein of 670kD composed of two upregulate thrombomodulin activity in endothelial cells. Thrombomodulin identical subunits and is synthesised by the follicular epithelial cells of the has been identified within a number of normal tissues. These include the thyroid. Thyroglobulin provides iodination sites for the formation of the lining cells of arteries, veins, capillaries and the lymphatics as well as thyroid hormones. mesothelial cells, meningeal lining cells, synovial cells, syncytiotro- Refer to page 42 for the Bond ready-to-use format. phoblasts, megakaryocytes and platelets. It is reported that in oral squamous cell carcinomas, the reduction of thrombomodulin expression may play a role in metastasis. Thrombomodulin protein is reported to be absent from most pulmonary adenocarcinomas but is expressed in malignant pleural mesotheliomas, vascular tumors and choriocarcinomas.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. sn C-S.Nt yolsi tiigo rprino neirptiaycls Paraffin cells. pituitary anterior of proportion a section. of staining hormone cytoplasmic stimulating Note thyroid NCL-TSH. for using staining immunohistochemical gland: pituitary human Normal format. ready-to-use Bond the is for pituitary 42 page and TSH to pituitary Refer hormones. the of thyroid cells which thyrotrophic of kD in 28 adenomas. production expressed of be and hormone to pituitary reported growth a thyroid is (TSH) stimulates hormone stimulating Thyroid PA0776 ready-to-use Bond mL 7 NCL-TSH lyophilized mL 1 QB2/6 Clone Hormone Stimulating Thyroid section. Paraffin cells. epithelial thyroid of staining cytoplasmic using intense peroxidase Note thyroid NCL-L-TPO. for staining immunohistochemical disease: Grave’s Thyroid, diluent and buffer wash TBS-based systems. in thyroid used when optimally in stains NCL-L-TPO differences quantitative Information Specific tissue. as Product normal to well compared cancer Thyroid thyroid as in studies expression Some of qualitative, peroxidase cells. follicular expression all shown in thyroid stain have thyroid, cytoplasmic produce of granular to fine, normal reported regulation diffuse, is a immunohistochemically In the described under (TPO) (TSH). Peroxidase is hormone expression stimulating gene Peroxidase Thyroid NCL-L-TPO liquid mL 1 AC25 Clone Peroxidase Thyroid (HIER) P (Enzyme) P F (Enzyme) P eetruigNLTHR.Nt yolsi tiigo hri pteilcls Paraffin cells. epithelial thyroid of section. staining cytoplasmic hormone Note stimulating NCL-TSH-R2. thyroid using for receptor staining immunohistochemical gland: thyroid human Normal is thyroid disease. Graves' the in TSHR of increased surface is cell and carcinoma. cell conditions basal papillary neoplastic the lateral in medullary in of cells essentially follicular and metaplasia and preserved be apical the cell to carcinoma of squamous reported in surface in anaplastic observed cell absent carcinoma, expression basal is no the Expression along surfaces. with exclusively is is cells TSHR it detected tissues, humans follicular be thyroid in human and to normal thyroid In normal reported target. of molecule function autoimmune and an important growth frequently an of is control (TSHR) the receptor for hormone stimulating thyroid The NCL-TSH-R2 lyophilized mL 1 4C1/E1/G8 Clone Receptor Hormone Stimulating Thyroid ee opg 2frteBn ed-oueformat. ready-to-use Bond the for 42 page and thyroid to Refer anaplastic carcinomas in follicular not papillary, but thyroid, in the expressed carcinomas. of swelling be of non-malignant to TTF- a percent carcinoids. reported goitre, pulmonary 90 also typical non-small in than is expressed pulmonary greater not 1 and of is percent in it lungs 75 but expressed in carcinomas and cell and be carcinomas thyroid to cell abnormalities. small the reported congenital pulmonary been of of number has development a TTF-1 underline proper may the expression to abnormal TTF-1 for shown have its essential may studies to targeting TTF-1 be addition Gene organs, organogenesis. In adult in by in protein. function activator regulated the also transcriptional be of tissue-specific may a status by that as phosphorylation role manner functions and a TTF-1 redox in percent proteins. the sites 98 both mouse sharing recognition and acids specific amino rat to Human thyroid 371 binding equivalent proteins. of the thyroglobulin, polypeptide surfactant with single include a and homology is These protein kD) brain. (38 secretory expressed TTF-1 and genes cell homeodomain regulating Clara lung the in peroxidase, of thyroid, role a member the plays a within and is family factor (TTF-1) transcription factor-1 transcription Thyroid PA0364 ready-to-use Bond mL 7 NCL-L-TTF-1 liquid mL 1 NCL-TTF-1 lyophilized mL 0.1 mL, 1 SPT24 Clone Factor-1 Transcription Thyroid W P F etr blotting Western Paraffin Frozen C I HE)W (HIER) P F lwcytometry Flow Immunofluorescence FP (HIER) P HE)W (HIER) P F Reference Range O E te applications Other lcrnmicroscopy Electron 163 /
Primary Antibodies Human pulmonary adenocarcinoma: immunohistochemical staining for thyroid transcription Human small intestine: immunohistochemical staining for tissue inhibitor of matrix factor-1 using NCL-TTF-1. Note nuclear staining in a proportion of tumor cells. Paraffin section. metalloproteinase 1 using NCL-TIMP1-485. Note cytoplasmic staining of epithelial cells. Paraffin section.
Tissue Inhibitor of Matrix TNFR1-Associated Death Domain Metalloproteinase Antibodies Protein Clone 6F6a 1 mL lyophilized Tissue Inhibitor of Matrix Clone 18A11 Metalloproteinase 1 NCL-TIMP1-485 P (HIER) W 1 mL lyophilized NCL-TRADD P (HIER) TNFR1-Associated Death Domain Protein is also known as TRADD. Death Clone 46E5 receptors of the TNF receptor family contain an intracellular death domain 1 mL lyophilized Tissue Inhibitor of Matrix that serves to recruit adaptor proteins such as TRADD and FADD and cysteine proteases such as caspase-8. TRADD is a 34 kD protein containing Metalloproteinase 2 NCL-TIMP2-487 P (HIER) W a death domain within its C-terminus which associates with the death domain of TNFR1. Activation of TNFR1 or overexpression of TRADD induces Primary Antibodies Clone 18D12b two opposite signalling pathways, caspase activation of apoptosis induction 1 mL lyophilized Tissue Inhibitor of Matrix and NF-kappaB activation for anti-apoptosis gene upregulation. Stat1 directly interacts with TNFR1 and TRADD, but not with FADD, and acts as a Metalloproteinase 3 NCL-TIMP3 FP TNFR1-signalling molecule to suppress NF-kappaB activation. TRADD is also The tissue inhibitors of metalloproteinases (TIMPs) are natural inhibitors of known to interact with the Epstein-Barr virus (EBV) latent membrane protein matrix metalloproteinases (MMPs), a group of zinc-binding endopeptides 1 (LMP1), the major oncogene of EBV. Human TRADD is reported to be involved in degradation of the extracellular matrix (ECM). The remodelling of constitutively expressed at low levels in all tissues, while mouse TRADD the ECM in a controlled fashion is essential during normal development and mRNA is expressed at higher levels in spleen, lung, liver and kidney and at is a feature of physiological remodelling such as in wound healing. Tumor lower levels in brain, skeletal muscle and testis. cell invasion and metastasis closely correlate with the activities of two MMPs, MMP2 and MMP9, both of which degrade type IV collagen in basement membranes. TIMPs constitute a family of at least four types of protein of which two of these are expressed in a wide variety of cell types. Although TIMP2 inhibits all types of activated MMPs to varying degrees, it is TNF-Related Apoptosis-Inducing Ligand known to preferentially inhibit MMP2. TIMP2 also binds to proMMP9 and proMMP2 to form stable complexes in which activation of the proenzymes (TRAIL) occur. Studies have revealed that TIMP2 can inhibit the invasive potential of tumor cells in vitro and their metastatic phenotype in vivo. TIMP3 is secreted Clone 27B12 into the ECM and complexes with MMP1, 3, 7, 9, 13, 14 and 15 deactivating 1 mL lyophilized NCL-TRAIL P (HIER) W them irreversibly. TIMP3 mRNA is highly expressed in placenta but is also reported to be found in the heart, kidney, lung, pancreas, uterus and skeletal TRAIL (TNF-related apoptosis-inducing ligand), or APO-2L, is a 281 amino muscle with low levels in the brain. In early placenta, TIMP2 and TIMP3 acid cytotoxic protein closely related to Fas/APO-1 ligand with the mRNAs are reported to be found in cells of cytotrophoblastic columns and characteristic structure of a type II membrane protein. TRAIL induces decidual membrane. In endometrium, TIMP3 is reported to be expressed in extensive apoptosis in lymphoid as well as nonlymphoid tumor cell lines. luminal epithelium, glands, stroma, endothelial cells and vascular smooth Two TRAIL membrane receptors, DR4 and DR5, have been identified which muscle cells. In adult rat cerebellum, TIMP3 is reported to be expressed in are capable of mediating apoptosis and are distinct from Fas/APO-1 and TNF Purkinje cell somata and processes. TIMP3 is expressed by fibroblast-like receptors. TRAIL-induced apoptosis in target cells is mediated by the cells in ulcerated intestinal wall and in surrounding vessels and sweat activation of caspases. Normal tissues are resistant to TRAIL as they are glands during wound healing in skin. In breast carcinoma, TIMP3 mRNA reported to express high levels of decoy membrane receptors, DcR1/TRID expression is described in fibroblastic cells within the tumor stroma and DcR2/TRUNDD which antagonize TRAIL-induced apoptosis. TRAIL adjacent to cancer cells. induces apoptosis in a number of different tumor cell types because most transformed cells express little DcR1. TRAIL mRNA is expressed in spleen, lung, prostate, ovary and bowel with little expression in testis, heart, skeletal muscle and pancreas. TRAIL protein is reported to be expressed on CD4 and CD8 positive T lymphocytes following activation and is also predominant in first trimester placental syncytiotrophoblasts as well as Hofbauer cells.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. ooetltmr oprdwt omlclnmcs sarsl of result a as mucosa colon normal stability. mRNA or with transcription increased compared tumors colorectal a navreefc nsann.Ol rscnann ahbfesor buffers wash Tris-containing Only staining. used. on be I should effect NCL-TOPO diluents with adverse diluents or an buffers has wash DNA phosphate-containing of use of The levels Information Specific Product Elevated antibiotics. and by with detected drugs individuals I, of of topoisomerase also sera anti-tumor and DNA the erythematosus lupus in some systemic cells, autoantibodies eg of diseases RNA have eukaryotic targets II autoimmune and certain be enabling I cycle, to topoisomerases normal cell DNA found the Both DNA been alpha. across In II fluctuations topoisomerase the relevant DNA properly. show unlike not in does function I it stresses topoisomerase where in to synthesis located superhelical is RNA enzyme active polymerase the undergoing reduces that are shown that been probably genome has the It supercoiled of reaction. re-ligated negatively the regions then of is and which end positively nick the single-stranded at relaxing transient a of performing cellular a by capable is DNA of I protein topoisomerase Human variety kD transcrip- mitosis. 100 at replication, a segregation DNA and in condensation, recombination tion, chromosomal involved as enzymes such activities nuclear are Topoisomerases I NCL-TOPO lyophilized mL 1 1D6 Clone I Topoisomerase malignant of proportion a section. of Paraffin staining cells. cytoplasmic Note NCL-TRAIL. using apoptosis- ligand TNF-related inducing for staining immunohistochemical carcinoma: prostatic Human 32 rnfrasy,hv enrpre in reported been have assays, transfer P (HIER) P F C-OOI.Nt nes ula tiigo ainn el n cainlmitotic occasional and cells malignant of staining using nuclear alpha intense II Note topoisomerase NCL-TOPOIIA. for staining immunohistochemical tumor: bladder Human of status proliferation the than with associated expressed is widely is and activity cycle more and cell cells. expression is the alpha to in II beta linked Topoisomerase reported II alpha. been II has topoisomerase have topoisomerase tumors protein breast that primary this of analysis of indicated the expression of Reports cancer of tumors. different range for many significant the used A agents. in drugs chemotherapeutic variation several anti-cancer DNA predominant to the resistance many in is of alpha for mechanism involved II topoisomerase target enzyme of expression nuclear a Decreased therapy. essential is an and is replication alpha II Topoisomerase NCL-TOPOIIA lyophilized mL 0.1 mL, 1 3F6 Clone Alpha II Topoisomerase C-G oesann fteprstsi netdaes aafnsection. Paraffin areas. infected in parasites the using of antigen P30 staining gondii Note Toxoplasma NCL-TG. for staining immunohistochemical brain: human Infected a is P30 gondii. production. in Toxoplasma is vaccine of strains for most tachyzoites infection subunit between have antigen gondii to potential conserved reported highly a been life-threatening Toxoplasma has as antigen of This interest invasion. and generated of antigen steps as first severe surface the to fetus in referred involved major cause also P30, the the that also shown SAG-1, been infect may has in It can but hosts. which immunocompromised defects asymptomatically, parasite protozoan often congenital ubiquitous humans, a healthy is gondii Toxoplasma NCL-TG lyophilized mL 1 TP3 Clone gondii Toxoplasma section. Paraffin figures. W P F etr blotting Western Paraffin Frozen C I (HIER) P lwcytometry Flow Immunofluorescence 3 Antigen P30 HE)W (HIER) P F O E te applications Other lcrnmicroscopy Electron 165 /
Primary Antibodies Transforming Growth Factor Beta Troponin Antibodies
Clone TGFB17 Clone 1A2 1 mL, 0.1 mL lyophilized NCL-TGFB P (HIER) 1 mL lyophilized troponin C NCL-TROPC P Transforming growth factor beta (TGFB) is a potent cell regulatory polypeptide homodimer of 25 kD. It variably affects cell growth, different- Clone T1/61 iation and other aspects of cellular metabolism such as extracellular matrix 1 mL lyophilized troponin C NCL-TROPT F P (Enzyme) W production. Its effect depends upon the cell type and other growth factors Troponin comprises three protein subunits, troponin C, troponin I and present but in general, TGFB inhibits the growth of epithelial cells and troponin T. It is a contractile protein which comprises 5 percent of muscle stimulates the growth of mesenchymal cells. Most breast lesions, benign proteins. Troponin C, an 18 kD protein, binds calcium and is responsible for and malignant, involve abnormal proliferation and altered architecture of regulating the process of thin filament activation during skeletal muscle stromal and/or epithelial elements. Inflammatory cells present in the earliest contraction. Troponin I, a 21 kD protein, is the inhibitory subunit of the lesions of progressive systemic sclerosis (PSS) are reported to release complex and troponin T is responsible for binding the troponin subunits to TGFB possibly resulting in chemotactic recruitment of additional chronic tropomyosin, a 66 kD protein that links the troponin complex to the actin inflammatory cells. Platelets, a rich source of TGFB, are known to exhibit helix. The troponin C gene is reported to be expressed in three distinct aggregability and may contribute to the etiology of PSS. striated muscle lineages; cardiac myocytes, embryonic fast skeletal myotubules and adult slow skeletal myocytes. Reports have indicated that cardiac myofibers from cardiomyopathic rodent models display decreased Ca2+ sensitivity and that this property is a result of an alteration in the troponin/tropomyosin regulatory complex in the fibers. Product Specific Information NCL-TROPT reacts with human and chicken fast muscle troponin, but not slow muscle troponin T.
Human breast carcinoma: immunohistochemical staining for transforming growth factor beta
Primary Antibodies using NCL-TGFB. Note cytoplasmic staining of tumor cells. Paraffin section.
Transforming Growth Factor Beta Receptor (Type 1) Human skeletal muscle: immunohistochemical staining for troponin T using NCL-TROPT. Note intense staining of fast muscle fibers. Paraffin section. Clone 8A11 1 mL, 0.1 mL lyophilized NCL-TGFBR1 P (HIER) Transforming growth factor beta (TGFB) is a potent cell regulatory polypeptide homodimer of 25 kD. It variably affects cell growth, different- Tuberin iation and other aspects of cellular metabolism such as extracellular matrix production. Its effect depends upon the cell type and other growth factors present but in general, TGFB inhibits the growth of epithelial cells and Clone 3B4 stimulates the growth of mesenchymal cells. Most breast lesions, benign 1 mL lyophilized NCL-TUBERIN P (HIER) and malignant, involve abnormal proliferation and altered architecture of Tuberin is the 180 kD protein product of the tuberous sclerosis (TSC-2) gene stromal and/or epithelial elements. Platelets, a rich source of TGFB, are located on chromosome 16. Tuberous sclerosis is an autosomal disorder known to exhibit aggregability and may contribute to the etiology of PSS. known as phakomatosis which is characterized by the widespread develop- ment of benign growths, usually described as hamartomata, in many tissues and organs. Tuberin is widely expressed at low levels in most human tissues, with increased expression in cortical neurons, cerebellar Purkinje cells, motor neurons of the spinal cord, pancreatic islet B cells, cardiac muscle and small blood vessels of the kidney and skin.
Human colon, ulcerative colitis: immunohistochemical staining for transforming growth factor beta receptor (type 1) using NCL-TGFBR1. Note membrane staining of a proportion of epithelial cells and lymphocytes. Paraffin section.
Human cerebellum: immunohistochemical staining for tuberin using NCL-TUBERIN. Note cytoplasmic staining of Purkinje cells. Paraffin section.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. C--RF1san pial hnue nTSbsdws ufrand buffer wash TBS-based in used systems. when diluent optimally stains NCL-L-TRAF-1 The with Virus concert Epstein-Barr apoptosis. Information by Specific in induced Product mediated act be to some to TNF-alpha reported known is of suppress (EBV). protein is this activation to B of the expression kappa proteins to factor The additional Jun-N-kinase. nuclear hetero- leading and B of or kappa proteins factor activation homodimerize nuclear Traf as can such factors other transcription Traf-1 with responses. dimerize cellular to bind that some that proteins additional proteins mediate with of complexes family signalling forming a receptors are surface (Traf) factors receptor-associated TNF NCL-L-TRAF-1 liquid mL 1 7C11 Clone 1 Factor Associated Receptor- Factor Necrosis Tumor section. Paraffin cells. malignant of staining cytoplasmic using intense receptor Note lipoprotein NCL-TUB-B2. II beta tubulin immunohistochemical neuroblastoma: Human nestin. or vimentin with cross-react not does KNY-379 developing Clone in and Information Specific system endothelia. Product vascular nervous and and chondrocytes rat cells cells, been muscle developing ganglion also smooth and the has nerves, skeletal Expression of peripheral brain. organs neuroblasts, in been sensory has as migrating isoform well in this as tissues, that reported muscle fetal report In skeletal expressed testis. rats in tubulin and reported in beta brain of gland, Studies proportion adrenal microtubules. significant the in a most of represents the II construction of subunits beta one the beta tubulin is and in Tubulin alpha used tubulin. where of families are subunits multiprotein four of of classes one widespread is II beta Tubulin NCL-TUB-B2 lyophilized mL 1 KNY-379 Clone II Beta Tubulin (HIER) P HE)W (HIER) P ee opg 3frteBn ed-oueformat. ready-to-use Bond the for 43 page to Refer is expression its whose melanomas. and and tyrosinase melanocytes biosynthesis by in catalyzed melanin reported of is for is dopaquinone substrate family to deficiency oculocutaneous initial conversion particular Tyrosinase the a in is tyrosinase. and L-tyrosine involves albinism is albinism. of which forms melanocytes various of with within member associated key melanin a of enzymes, biosynthesis The PA0322 ready-to-use Bond mL 7 RTU-TYROS ready-to-use mL 7 NCL-L-TYROS liquid mL 1 NCL-TYROS lyophilized mL 1 T311 Clone Tyrosinase section. Paraffin cells. Sternberg Reed of staining NCL-L-TRAF-1. membrane using intense protein Note Traf-1 for staining immunohistochemical Lymphoma: Hodgkin’s oectpamcsann ftmrcls aafnsection. Paraffin cells. tumor of RTU-TYROS. staining using cytoplasmic tyrosinase Note for staining immunohistochemical melanoma: malignant Human W P F etr blotting Western Paraffin Frozen C I (HIER) P F lwcytometry Flow Immunofluorescence (HIER) P F (HIER) P F (HIER) P O E te applications Other lcrnmicroscopy Electron 167 /
Primary Antibodies Tyrosinase-Related Protein-1 Ubiquitin
Clone G3E6 Clone FPM1 1 mL lyophilized NCL-TRP-1 P (HIER) 1 mL lyophilized NCL-UBIQm P Tyrosinase-related protein-1 (TRP-1) is a member of a family of proteins Polyclonal which are involved in melanin biosynthesis. The catalytic function of TRP-1 has not been fully resolved but the enzyme appears to be important in the 1 mL lyophilized NCL-UBIQ FP oxidation of 5,6-dihydroxyindole-2-carboxylic acid to form a high molecular Ubiquitin, a small protein consisting of 76 amino acids, has been reported to weight pigmented biopolymer. In mammals, there are two basic types of be found in all eukaryotic cells studied. It is one of the most conserved melanin, the brown-black eumelanin and the reddish-yellow phaeomelanin. proteins known. Ubiquitin is required for ATP-dependent, non-lysosomal The concentrations of each are variable and are not related to skin type. In intracellular protein degradation, which eliminates most intracellular skin exposed to suberythemal doses of UVB, an increase in the number of defective proteins as well as normal proteins with a rapid turnover. melanocytes expressing TRP-1 and TRP-2 is reported with no increase in the Degradation involves covalent binding of ubiquitin to the protein to be number of tyrosinase-expressing melanocytes. In normal, untreated skin the degraded and it is believed that in this way ubiquitin acts to label the protein number of melanocytes that express either TRP-1, TRP-2 or tyrosinase are for disposal by intracellular proteases. The most abundant ubiquitin-protein similar irrespective of skin type. TRP-1 is also reported to be expressed in conjugate, however, is ubiquitin-histone H2A. This conjugate is not more than 50 percent of choroidal melanocytes in the adult eye. degraded. Since such ubiquitinated histones are present primarily in actively transcribed chromosomal regions, ubiquitin may play a role in regulation of gene expression.
Utrophin
Clone DRP3/20C5 2.5 mL, 1 mL lyophilized NCL-DRP2 FE Utrophin, located on chromosome 6, is a ubiquitously expressed homologue
Primary Antibodies Human malignant melanoma: immunohistochemical staining for tyrosinase-related protein-1 of dystrophin and is known as dystrophin-related protein. In normal muscle, using NCL-TRP-1. Note cytoplasmic staining of tumor cells. Paraffin section. utrophin is restricted to neuromuscular junctions. However, in dystrohin- deficient muscle, utrophin expression is reported to be upregulated and appears around the periphery of most fibres. Utrophin has a role as a cell anchoring molecule. The amino terminal region of utrophin binds to the actin cytoskeleton, acting as an intracellular anchor whereas the carboxyl Tyrosine Hydroxylase terminal regions bind to a group of proteins anchored in the cell membrane. Clone 1B5 1 mL, 0.1 mL lyophilized NCL-TH P (HIER) W Tyrosine hydroxylase is the first enzyme in catecholamine (CA) biosynthesis Varicella-zoster virus and catalyzes the conversion of L-tyrosine to L-DOPA. Tyrosine hydroxylase is reported to be expressed in all CA neurons. Despite the abundant data Clone C90.2.8 about the distribution of catecholaminergic neurons in a wide variety of species, data on their distribution in the human brain is less comprehensive. 1 mL lyophilized Varicella-zoster virus NCL-VZV PI However, one such study has reported that tyrosine hydroxylase products in Varicella-zoster virus is a member of the alphaherpesvirinae. It is respons- the substantia nigra were restricted to neural bodies, axons and dendrites. ible for two ubiquitous diseases: varicella (chickenpox), an exanthem of These in turn were restricted to the third decade of life and their number childhood, and herpes zoster (shingles), a disabling disease of the elderly increased in this location with age. This finding may be related to ageing of and immunocompromised individuals. melanin-pigmented neurons. Product Specific Information Product Specific Information NCL-VZV is specific for Varicella-zoster virus and does not cross-react with NCL-TH is reactive with tyrosine hydroxylase in human, mouse and rat brain Respiratory syncytial virus, Parainfluenza virus types 1, 2, 3 and 4b, tissue. Adenovirus, Herpes simplex virus types 1 and 2, Influenza virus types A and B, Mumps virus, Measles virus, echovirus 19, Coxsackie B4 virus and Poliovirus types 1, 2 and 3.
Human midbrain: immunohistochemical staining of tyrosine hydroxylase enzyme using NCL-TH. Note cytoplasmic staining of catecholaminergic cells and their processes. Paraffin section (Peroxidase substrate: nickel DAB, Counterstain: eosin). Human skin: immunohistochemical staining for Varicella-zoster virus using NCL-VZV. Note staining of infected cells within a vesicle. Paraffin section.
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. oesann fmlgatedteilcls aafnsection. Paraffin cells. endothelial malignant of NCL-L-VE-Cad. staining using Note VE-Cadherin for staining immunohistochemical angiosarcoma: Human is derived tumors VE-Cadherin identify to endothelial placenta. used the tissue. be in endothelial and to only from reported expressed vessels been is has it lymphatic VE-Cadherin VE- as layers. proteins and matrix. adherin the blood extracellular among dependant unique such the of layers, calcium endothelial to those of junctions cells a as intracellular the adhesion to is localized the is Cadherin in (VE-Cadherin) involved cadherin molecule endothelial Vascular NCL-L-VE-Cad liquid mL 1 33E1 Clone (CD144) VE-Cadherin section. Paraffin syncytiotrophoblasts. of staining NCL-L-VEGFR-3. cytoplasmic using Note VEGFR-3 for staining immunohistochemical placenta: Human and growth. tumor endothelium during angiogenesis capillary in being involved blood be in to whilst In thought reported an vessels. is cells, VEGFR-3 been lymphatic play and to has blood endothelial appears expression of development It tumors, normal most development. the in in in role later important embryogenesis, vessels expressed lymphatic intestine, Throughout to is lung, restricted including (syncytiotrophoblasts). mRNA tissues VEGF-D. placenta many VEGFR-3 and in VEGF-C and found as be known brain are to and reported have VEGF ligands is to known VEGFR-3 homology two of the VEGFR-1 degree However, to VEGF. high structure bind a in not similar does but kinase VEGFR-2 tyrosine and receptor a is (FLT4) VEGFR-3 NCL-L-VEGFR-3 liquid mL 1 KLT9 Clone Receptor-3 Factor Growth Endothelial Vascular (HIER) P P el ftesi n ouaino eta,prpea n nei neurons. enteric and peripheral central, of Langerhans population a pancreas, and the skin in the of all expressed of cells be cells in to beta reported cells, coexpressed also is enterochromaffin-like the be It gastric medulla. of to the adrenal membranes the reported of the from cells is in chromaffin located VMAT2 accumulation monoamine is granules/vesicles. monoamine and Vesicular secretory organelles the determine. storage into to mediates cytoplasm difficult (VMAT2) be and origin transporter may whose tumors, behavior endocrine in produc- biological demonstrated hormone be always Specific cannot using immunohistochemistry. tion reported been or has differentiation. microscopy cells neuroendocrine tumor electron of and in granules evidence in secretory cells as reported of is regarded Demonstration (EC) vesicles is secretory and enterochromaffin of cells secretory presence tumor in of The cells. membranes for expressed chromaffin the exchange adrenal is in in located and organelle glycoprotein, mediate storage granules/vesicles a the is into VMAT2) VMAT1 substrates protons. amine transport to and cationic gradient proton the TheyATPase-generated vacuolar organelles. (VMAT1 a storage on into dependent are cytoplasm transporters the from accumulation monoamine monoamine Vesicular 2 Transporter NCL-L-VMAT2 Monoamine (VMAT2) Vesicular liquid mL 0.1 mL, 1 136 NN Clone 1 Transporter NCL-L-VMAT1 Monoamine (VMAT1) Vesicular liquid mL 0.1 mL, 1 RMT77 Clone Antibodies (VMAT) Transporter Monoamine Vesicular fmlgatcls aafnsection. staining Paraffin cytoplasmic cells. intense malignant Note of NCL-L-VMAT1. using vesicular (VMAT1) for 1 staining transporter immunohistochemical monoamine bowel: the of carcinoid infiltrating Human W P F etr blotting Western Paraffin Frozen C I (HIER) P (HIER) P lwcytometry Flow Immunofluorescence O E te applications Other lcrnmicroscopy Electron 169 /
Primary Antibodies Villin
Clone CWWB1 1 mL, 0.1 mL lyophilized NCL-VILLIN F P (HIER) W 1 mL liquid NCL-L-VILLIN F P (HIER) W 7 mL Bond ready-to-use PA0106 P (HIER) Villin and the structurally-related proteins gelsolin, fragmin and severin, all regulate the framework and assembly of actin. Villin is unique among these proteins in its ability to cross-link actin filaments into bundles, a process observed only at low Ca2+ concentration. Villin is composed of three Astrocytoma: immunohistochemical staining for vimentin using NCL-L-VIM-572. domains. The first two domains are homologous and the third domain is Note intense cytoplasmic staining of astrocytes. Paraffin section. called the “headpiece”. This “headpiece” region is located at the C-terminus. Villin is mainly produced by epithelial cells that develop a brush border. Cells producing villin are reported to be found either in the epithelial cells of the intestinal mucosa and gall bladder, or in epithelial cells of the kidney proximal tubules and ductuli efferentes of the testis. However, villin is also WAF1 Protein (p21, C1P1) reported to be found in some epithelia which lack a brush border but which are derived from embryonic gut such as duct cells of the exocrine pancreas and biliary cells of the liver. In these cell types, villin is concentrated in the Clone 4D10 apical cytoplasm. Epithelial cells of the intestinal mucosa are continually 1 mL, 0.1 mL lyophilized NCL-WAF-1 P (HIER) being renewed and this involves a migration of these cell types from the 1 mL liquid NCL-L-WAF-1 P (HIER) intestinal crypts to the tips of the villi, gradually acquiring their differentiated phenotype as they do so. The maximum production of villin occurs at the The gene encoding WAF1, also termed p21, is transcriptionally regulated by base of the villus. Villin, therefore, shows tissue-specific expression being the suppressor protein, p53. Overexpression of WAF1 is growth suppressive, restricted to certain epithelia and their apical domains, thus indicating their possibly by inhibiting the activity of cyclin/CDK complexes. One polarity. The morphological loss of polarity of colonic epithelial cells is consequence of WAF1 binding to cyclin/CDK complexes is the inhibition of reported to be one of the most significant indicators of dysplasia or Rb protein phosphorylation. Induction of WAF1 expression requires wild neoplasia. type p53 activity in cells undergoing p53 dependent G1 arrest or apoptosis. Mutation of the p53 gene is a common event in human cancer and results in
Primary Antibodies Refer to page 43 for the Bond ready-to-use format. the failure to produce WAF1. The effect of this may lead to uncontrolled cell proliferation.
Vimentin Wilms' Tumor Clone SRL33 1 mL, 0.1 mL liquid NCL-L-VIM-572 P (HIER) W Reference Range Clone WT49 7 mL Bond ready-to-use PA0033 P (HIER) 1 mL, 0.1 mL liquid NCL-L-WT1-562 P (HIER) Reference Range 7 mL Bond ready-to-use PA0562 P (HIER) Clone V9 Wilms' tumor protein (WT1) has a role in transcriptional regulation and is 1 mL, 0.1 mL lyophilized NCL-VIM-V9 F P (HIER) W expressed in the kidney and a subset of hematopoietic cells. Alteration of 1 mL liquid NCL-L-VIM-V9 F P (HIER) W transcription factor function is a common mechanism in oncogenesis. The 7 mL ready-to-use RTU-VIM-V9 F P (HIER) WT1 protein contains a DNA binding domain and any deletions or point mutations of the WT1 gene which destroy this activity result in the development of the childhood nephroblastoma Wilms' tumor and Denys- Clone VIM 3B4 Drash syndrome. The description of WT1 involvement in nephroblastoma is 1 mL lyophilized NCL-VIM F P (Enzyme) W not clear. Expression of WT1 protein by immunohistochemistry is never Eukaryotic cells contain a number of types of cytoplasmic filamentous observed (or exceptionally rare) in breast cancer and negativity is proteins, microtubule, microfilaments and intermediate-sized filaments (IF). considered a useful marker to distinguish breast carcinoma metastases Vimentin, a 57 kD protein that is an intermediate filament is reported to be from serous ovary primary carcinomas. This is at variance with observations expressed in most cells of mesenchymal origin, including fibroblasts, regarding mRNA in these tumors, but this is a still unresolved and complex endothelial cells, smooth muscle, melanocytes as well as T and B issue. lymphocytes. Product Specific Information Refer to page 43 for the Bond ready-to-use format. Endothelial cells are prevalently negative using clone WT49. These cells are otherwise immunoreactive with clone 6FH2. Refer to page 44 for the Bond ready-to-use format.
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Primary Antibodies Primary Antibodies
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For detailed information on all products please visit our website: Products in this catalog are subject to regulatory approval. www.leica-microsystems.com This catalog is not for use in the USA. Novocastra ancillary reagents. ancillary Novocastra Novolink on rely always reagents, primary Novocastra your from most the get –to compromise Don’t MANUAL DETECTION SYSTEMS NOVOCASTRA aulDtcinSystems Detection Manual TM detection, Novocastra diluent, and and diluent, Novocastra detection, TM W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence O E te applications Other lcrnmicroscopy Electron 173 /
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Primer Sets Primer Sets o ealdifraino l rdcspes ii u website: our visit please www.leica-microsystems.com products all on information detailed For 188 / NOVOCASTRA PRIMER SETS NOVOCASTRA advice. and sample preparation instructions detailed and cyclers comes with aircapillary thermal as wellas block heated instruments, inconventional has Each beentested set primer cost. low at test onan arapid, sensitive agarose mayberesolved gel, giving products PCR The size. different a of products yield but same primers the with co-amplify DNA control and internal DNA target The template. control and an internal primers oligonucleotide apair of of consist normally Novocastra from Sets Primer included test. inthe isroutinely control (amplification) an internal unless unrecognized pass results Such false-negative method. this of sensitivity exquisite potentially the of are common leading considerable to blunting chain (PCR) reaction polymerase the of Inhibitors DETECTION FOR REAGENTS -ADVANCED SETS PRIMER Primer 1 ++ ++ Primer Set Primer 2 Template Control Control Sample hsctlgi o o s nteUSA. the in use for not approval. is regulatory catalog to This subject are catalog this in Products the amplification anddetectionofmicrobial,orviralDNAby A schematicdiagramillustratingtheuseofaPrimerSetfor the PCRprocess.LaneA,DNAladder. LaneB,sample. PCR Lane A Lane B - Controlband - Positiveband n.adHfmnL-oh t.Prhs fteepout osntcryalcneto licence a carry not does process. products PCR these Laroche the of Hoffman perform Purchase by Ltd. owned La-Roche patents Hoffman by and covered Inc. is process (PCR) The reaction isolate. chain diphtheriae Corynebacterium polymerase Corynebacterium toxigenic non-toxigenic five G, H, Lane and isolates. C-F ladder diphtheriae Lanes 100bp blank. A, Reagent Lane B, NCL-CD-PS. Lane using (Promega). products PCR of electrophoresis gel: Agarose but primers same the size. with different amplify will of for it product DNA where a PCR, yield control test the internal DNA to control an added internal The be is results. may PCR provided false-negative of of Also detection region the 246bp subunit. in aiding a alpha amplify gene which toxin CD-2) pair the and a (CD-1 of primers consists oligonucleotide NCL-CD-PS, of set, primer and diphtheriae Corynebacterium experience The require Information tests Specific Product toxin but non-toxigenic, western expertise. still Most diphtheriae. are led Corynebacterium have of isolates travel awareness modern and increased Europe an eastern to in diphtheria of outbreaks Large NCL-CD-PS set primer Tests 100 diphtheriae Corynebacterium O RUO nmn omnioae.As rvddi nitra oto N o aiding results. for PCR DNA false-negative control of internal detection an the is in provided Also copies isolates. multiple in common sequence many a in as present Mycobacterium is the and of (MTBC) members complex to tuberculosis unique is insertion sequence 123bp The the pair IS6110. amplify sequence a which MT-2) of and conventional (MT-1 consists primers NCL-MT-PS, oligonucleotide of set, primer by tuberculosis Mycobacterium The detected all later Information identify (but Specific tuberculosis certainly Product missed will PCR percent). Mycobacterium be 80 that to may (60 reported microscopy of infections is it level is but strains low sputum culture), Some in by Direct detected tuberculosis AIDS. PCR. Mycobacterium with by overlap of possible an identification drug-resistant and and of tuberculosis emergence detection an Mycobacterium tuberculosis, of of incidence strains rising a is There NCL-MT-PS set primer Tests 100 tuberculosis Mycobacterium fteepout osntcryalcnet efr h C process. PCR the perform to licence Purchase a Ltd. carry La-Roche not Hoffman does and products Inc. these Laroche of Hoffman is by process owned (PCR) patents reaction by chain covered polymerase The lung. human formalin- paraffin-embedded tuberculosis-positive, fixed, Mycobacterium H, Lane Mycobacterium G, sputa. Mycobacterium and human tuberculosis, F tuberculosis-positive Lanes Mycobacterium respectively. E, isolates, and intracellulare D Mycobacterium and C, avium Lanes (Sigma). only. ladder control 123bp internal A, Lane B, NCL-MT-PS. Lane using products PCR of electrophoresis gel: Agarose W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence O RUO O E te applications Other lcrnmicroscopy Electron 189 /
Primer Sets Primer Sets o ealdifraino l rdcspes ii u website: our visit please www.leica-microsystems.com products all on information detailed For 190 / hsctlgi o o s nteUSA. the in use for not approval. is regulatory catalog to This subject are catalog this in Products Medical Systems products. Systems Medical verifi Ventana equivalent with test cation alot-to-lot pass to proven independently platforms. staining BenchMark on Ventana use for clones Novocastra trusted and Familiar ORIGIN ® TM Medical Systems’ NexES Systems’ Medical Series immunohistochemistry immunohistochemistry Series Each antibody hasbeen antibody Each TM ® and and Origin W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence O E te applications Other lcrnmicroscopy Electron 191 /
Origin Origin o ealdifraino l rdcspes ii u website: our visit please www.leica-microsystems.com products all on information detailed For 192 / section. Paraffin skin. on JPM30) (Clone CD1a Origin including tissues, JPM30. neoplastic Clone about information and further for 70 normal page to Refer of variety thymomas. and histiocytosis a cell Langerhans’ protein in CD1a detecting in use expression for recommended is JPM30) (Clone CD1a Origin atherosclerotic Information in Specific CD1a Product of participation inflammation. asthmatic the and and formation in lesion cells disease Langerhans host epidermal in in versus reduction useful graft a reported be have studies antigen Immunohistochemical cortical CD1a cells. to for present interdigitating and from cells shown cells epithelia, presenting and dendritic been skin antigen on in powerful has expressed cells, kD expression Langerhans 49 differentiating antigen to 43 CD1a of thymocytes. protein a is CD1a ORG-8968 Tests 50 JPM30 Clone CD1a section. Paraffin tonsil. on bcl-2/100/D5) (Clone bcl-2 Origin bcl-2/100/D5. Clone about information further for 60 page to Refer nodes. lymph reactive differentiation from the lymphomas in with use of for recommended gene, is bcl-2/100/D5) (Clone bcl-2 immunoglobulin Origin an to Information Specific production Product 18q21, proliferation. cell cell and at in expression B gene gene inhibited of an deregulation the subsequent bcl-2 as is which occurs of act translocation the expression a part to lymphomas juxtaposes bcl-2 follicular forms considered of percent in reason is 90 apoptosis In involved It pathway. this where are distribution. For centers tissue that germinal apoptosis. wide proteins has of of protein and inhibitor family membrane kD mitochondrial a inner 25 integral of of an is member antigen a The apoptosis. is antigen Bcl-2 ORG-8714 Tests 50 bcl-2/100/D5 Clone Oncoprotein bcl-2 IVD IVD rgnC3(ln S)o osl aafnsection. Paraffin tonsil. on PS1) (Clone CD3 Origin PS1. Clone about information further for 70 using antibodies. page indicated of to panel Refer be a of may part as disorders PS1) (Clone lymphoproliferative CD3 in Origin phenotype cell T of indicators Information earliest Specific the Product of lineage. cell one normal thymocytes T represents early the in to in probably expressed commitment detected differentiation, first appearance is cell its antigen T CD3 and The of cells. marker T a neoplastic and is antigen CD3 The ORG-8982 Tests 50 PS1 Clone CD3 rgnC4(ln F)o tonsil. on 1F6) (Clone CD4 Origin (helper/inducer) subset cell including T positive. cell lymphoma, CD4 lower adult generally cell associated also T a T HTLV-1 is antigen. leukemia/lymphoma at cutaneous CD4 a the of and express cases fungoides, lymphocytes on mycosis Most blood monocytes. expressed peripheral on of level is percent 45 antigen representing CD4 The ORG-8756 Tests 50 1F6 Clone CD4 hsctlgi o o s nteUSA. the in use for not approval. is regulatory catalog to This subject are catalog this in Products IVD IVD rgnC8(ln A)o osl aafnsection. Paraffin tonsil. on 1A5) (Clone CD8 Origin 1A5. Clone about information further for 72 page to Refer of part as 1A5) (Clone antibodies. CD8 of Origin of panel using a characterized percent be may disorders 30 cell T of sub-population cells. Information marrow a Specific bone Product of on percent 15-30 thymocytes, killer and blood cells natural null of on peripheral blood detected peripheral percent human be to cells 80 of reported cytotoxic/suppressor percent is cells, normal molecule of 20-35 CD8 subset The approximately a lymphocytes. up on of found weight make molecular been a which has has and It chains kD. two of 32 composed is molecule CD8 The ORG-8936 Tests 50 1A5 Clone CD8 section. Paraffin spleen. on 4C7) (Clone CD5 Origin 4C7. Clone about information further for 71 page to Refer lymphomas, of lymphomas. diagnosis cell differential mantle the in including used be can 4C7) CD5 (Clone CD5 Origin lymphomas. cell T is Information Specific and expression Product lymphomas. B cell antigen follicular as in CD5 expressed well chronic not cells. cell is as B B antigen some (B-CLL) of (T-ALL), leukemias leukemias subset lymphocytic lymphocytic acute a lymphomas, cell cell on T 72 T in many and and reported by thymocytes cells expressed is of T antigen percent CD5 activated reactivity cells. 95 main T the on on nodes, observed expressed lymph is In kD, lymphocytes. blood 67 peripheral of of percent protein a is CD5 ORG-8919 Tests 50 4C7 Clone CD5 IVD IVD rgnC1 Coe5C)o ypoa aafnsection. Paraffin lymphoma. on 56C6) (Clone CD10 Origin 56C6. Clone about information further for of 73 page diagnosis leukemia. to differential lymphoblastic Refer of the subtyping in the used and lymphoma be cell may B 56C6) small (Clone as leukemia, CD10 myelocytic chronic Origin with such, patients follicular from and cells Burkitts’ B lymphoblastic, on and of lymphomas cells on reported is expression CD10 of Information variety Specific wide Product a on expressed cells. lymphoblastic is neoplastic acute and antigen normal common CD10 and (CALLA). neprilysin antigen as leukemia known also is antigen CD10 ORG-8941 Tests 50 56C6 Clone CD10 rgnC2 Coe11)o osl aafnsection. Paraffin tonsil. on 1B12) (Clone CD23 Origin 1B12. Clone about information further for 76 page to Refer diagnosis lymphomas. differential cell the B in small use for of recommended is 1B12) (Clone CD23 been Origin has Expression Information Specific cells. Product and B leukemias lymphocytic on chronic found cell EBV- lymphomas. B receptor centrocytic/centroblastic from on known cells IgE also and neoplastic on affinity is lymphocytes reported molecule low CD23 B the The cells, lines. as cell blood on lymphoblastoid found peripheral B reportedly transformed is of kD, 45 subpopulation of glycoprotein a membrane a antigen, CD23 The ORG-8826 Tests 50 1B12 Clone CD23 W P F etr blotting Western Paraffin Frozen IVD IVD C I lwcytometry Flow Immunofluorescence O E te applications Other lcrnmicroscopy Electron 193 /
Origin Origin o ealdifraino l rdcspes ii u website: our visit please www.leica-microsystems.com products all on information detailed For 194 / 34 (Clone Cytokeratin Origin 34 Clone about information further for 137 page to Refer of the is of lesions and gland. small-acinar epithelia malignant prostate and complex benign of from differentiation the arising in value carcinomas ductal and squamous 34 (Clone Cytokeratin Origin Information Specific approaching markers. Product for differentiation as basis cytokeratins a using tumors, as of serves characterization metastasis the and patterns growth polypeptide malignant cytokeratin during of the maintenance and tumors towards epithelial-derived tendency in general cytokeratins different of expression The ORG-8735 Tests 50 34 Clone Cytokeratin section. Paraffin tonsil. on 11E3) (Clone CD79a Origin 11E3. Clone about information further the for 84 in page used to Refer be may 11E3) disorders. (Clone cell CD79a B of Origin characterization including acute panel antibody of An in and majority lymphomas lines. cell the Information cell T intracellular Specific B or Product myeloid in an lines, in cell present as reported not B and found is types, It is is myelomas. cell some it B polypeptides antigen precursor where bound of stage CD79a of leukemias cell The membrane pre-B to complex the reported component. is at antigen the CD79a appear This this The CD79b. of first and components with CD79a two cells. designated The are receptor. complex cell associated B B the constitutes immunoglobulin This on noncovalently protein. heterodimer immunoglobulins linked is disulfide domain, protein cytoplasmic a is CD79a ORG-8975 Tests 50 11E3 Clone CD79a E12 1)o qaoscl acnm.Prfi section. Paraffin carcinoma. cell squamous on E12) IVD IVD 1)i eomne o h hrceiainof characterization the for recommended is E12) E12. ee opg 0 o ute nomto bu ln DE-R-11. Clone about information further for 101 page to muscle, Refer not smooth does antibody from proteins. The arising filament muscle. intermediate those striated other recognize from as derived such those origin, and leiomyomas myogenic of determination the in tumors aid an of as recommended is DE-R-11) (Clone Desmin when Origin appearance striated skeletal Information Specific in Product characteristic in bands Z protein a the filament giving to stained. confined intermediate immunohistochemically muscle is cytoplasmic It cardiac kD cells. and muscle 53 smooth a and striated is desmin Human ORG-8889 Tests 50 DE-R-11 Clone Desmin rgnEtoe eetr(ln F1 nbes acnm.Prfi section. Paraffin carcinoma. breast on 6F11) (Clone Receptor Estrogen Origin 6F11. Clone about information further for alpha 106 ER page to the tissues. Refer to neoplastic and specifically normal binds of it nuclei the cancer, and in breast prognosis antigen in in aid therapy an as of indicated prediction is 6F11) (Clone Receptor Estrogen Origin carried be Information Specific to Product qualitatively. status and ER quantitatively of laboratories histopathology determination routine the in important allowed out Monoclonal have an therapy. ER endocrine is to to tissue response antibodies cancer and prediction breast the in in parameter expression (ER) receptor Estrogen ORG-8871 Tests 50 6F11 Clone Receptor Estrogen section. Paraffin bowel. on DE-R-11) (Clone Desmin Origin hsctlgi o o s nteUSA. the in use for not approval. is regulatory catalog to This subject are catalog this in Products IVD IVD rgnMlnA(ln 13 nmlgatmlnm.Prfi section. Paraffin melanoma. malignant on A103) (Clone A Melan Origin a103. Clone about information further for 132 page to Refer A Melan of assessment the lesions. for melanocytic recommended in is A103) (Clone A Melan Origin roles Information particular Specific their Product of study the in Other help expression tumorigenesis. of their in be and may lymphocytes. molecules melanomas different BAGE-1 individual these gp75, in of T gp100, analysis tyrosinase, The MAGE-3, GAGE-1. cells cytotoxic MAGE-1, and differentiation T include melanocyte to cytotoxic autologous reported a autologous are by is recognized by gene, markers MART-1 melanoma-associated the recognized of product marker a A, Melan ORG-8953 Tests 50 A103 Clone A Melan section. Paraffin carcinoma. breast on MM1) (Clone Ki67 Origin MM1. Clone about information further for 126 page to Refer proliferation cell tissues. of neoplastic assessment and the normal in in utilized be can MM1) (Clone Ki67 Origin Information Specific Ki67 Product nucleus. with the the associated to strictly proteins confined is and Ki67 cycle associated of cell during presence the cycle detectable cells the not Thus, resting is cell processes. and in repair cells other not all DNA quiescent but in in many absent mitosis) consistently expressed and to is G2 is antigen contrast S, which (G1, In protein, cycle associated cell (G0). the cycle of cell parts nuclear active a is Ki67 ORG-8772 Tests 50 MM1 Clone Ki67 IVD IVD rgnMlnsm CoeHB5 nmlgatmlnm.Prfi section. Paraffin melanoma. malignant on HMB45) (Clone Melanosome Origin HMB45. Clone about information further for 120 page to Refer melanoma. of panel diagnosis a the of for part antibodies as use of for recommended is HMB45) (Clone Melanosome Origin are Information origin Specific mesenchymal Product and glial negative. lymphoid, infantile be and epithelial to prenatal reported of transient with epithelium cells only pigment Tumor reactive retinal be RPE. to in reported identified is but been (RPE) has antigen melanosome The ORG-8854 Tests 50 HMB45 Clone Melanosome rgnPoetrn eetr(ln 6 nbes acnm.Prfi section. Paraffin carcinoma. breast on 16) (Clone Receptor Progesterone Origin 16. Clone about information further for 152 page tissue. to cancer Refer for breast recommended of is status it alpha such receptor as progesterone tissues, the neoplastic determining and normal of antigen PRA nuclei the the to in a specifically binds 16) (Clone as Receptor Progesterone glucocorticoid Origin acts PRB, activity Information of Specific PRA Product transcriptional receptor. humans, mineralocorticoid the and the In receptor lacking androgen form, ER, of acids. receptor, PRB the amino repressor of version N-terminal transdominant transcription truncated ligand-activated 164 a as is function first form which PRA PRA kD), isoforms, The two (114 factors. as PRB expressed and is kD) (PR) (94 receptor progesterone human The ORG-8721 Tests 50 16 Clone Receptor Progesterone W P F etr blotting Western Paraffin Frozen IVD IVD C I lwcytometry Flow Immunofluorescence O E te applications Other lcrnmicroscopy Electron 195 /
Origin Origin o ealdifraino l rdcspes ii u website: our visit please www.leica-microsystems.com products all on information detailed For 196 / section. Paraffin leukemia. lymphoid on SEN28) (Clone TdT Origin SEN28. Clone about information further for 162 page to Refer leukemia/ lymphoblastic acute lymphomas. of other differentiation from recommended and lymphoma is diagnosis SEN28) the (Clone in lymphoma TdT use Origin lymphoblastic for such, between as it lymphoma, histologically when Burkitts’ valuable differentiate and most to be to difficult reported is is expression TdT of determination The Information and Specific thymus Product normal the leukemias. of and is cells lymphomas kD, T lymphoblastic 58 and acute of B marrow, primitive bone weight in molecular expressed a be with to polymerase reported DNA nuclear a TdT, Human ORG-8865 Tests 50 SEN28 Clone Transferase Deoxynucleotidyl Terminal section. Paraffin tumor. carcinoid on 27G12) (Clone Synaptophysin Origin 27G12. clone about information further for 160 page to Refer differentiation. and tumors origin of identification neuroendocrine as the of for recommended well is 27G12) (Clone as Synaptophysin and in medulla Information involved Specific Product adrenal be the may exocytosis. and and antigen in formation cord vesicle synaptophysin spinal synaptic vesicles The brain, similar the junctions. in neuromuscular in to neurons reported the and is of retina It vesicles cells. presynaptic neuroendocrine in neoplastic in occur and present glycoprotein normal membrane human integral many an is antigen Synaptophysin The ORG-8848 Tests 50 27G12 Clone Synaptophysin IVD IVD hsctlgi o o s nteUSA. the in use for not approval. is regulatory catalog to This subject are catalog this in Products PRODUCT NAME INDEX PRODUCT NAME rdc aeIndex Name Product W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence O E te applications Other lcrnmicroscopy Electron 197 /
Product Name Index Product Name Index o ealdifraino l rdcspes ii u website: our visit please www.leica-microsystems.com products all on information detailed For 198 / Calretinin Calcitonin CA125 Labels Slide Universal Bond Covertile Universal Bond Inserts Container Titration Bond Tray Slide Bond Pen Cleaning Labeler Slide Bond Tray Reagent Bond Detection Refine Polymer Bond Unit mL mL 30 7 Containers Open Bond Page Stations 14 Mixing Bond 2 Solution Retrieval Epitope Bond Code PA0963 Kit Pretreatment Enzyme Bond Enhancer DAB Bond System Cleaning Probe Aspirating Bond Bcl-6 (BOB-1) Protein-1 Binding Octamer Specific Cell B Antibody Anti-Fluorescein Kinase Lymphoma Anaplastic Fetoprotein Alpha Product Bond apnn(ai)P01 67mL 7 mL 7 16 17 PA0416 PA0004 and Containers Titration 10 L 1 Labels/roll 48 3000 mL 6.25 13 49 47 OPT9049 AR9590 or dispense) μL (100 Tests 300 S21.1985.110 Antigen Roll Carcinoembryonic 1 AR0633 (Basic) Calponin 12 mL 500 48 Lewis (Sialyl CA19-9 Concentrate 13 10X Solution Wash Bond S21.1912.110 Tests 100 DS9455 Covers Label Tests/ Slide 200 Universal minimum Bond Pack, 10 or 12 dispense) μL AR9352 (100 Tests 300 Kit Titration Bond 48 Wash Stringency Bond L 1 12 DS9390 Ribbon L Printing 1 Labeler Slide mL Bond 7 OP79193 Rack 1 13 Detection 14 Research Bond DS9263 15 mL 7 48 Diluent Antibody Primary Bond AR9222 15 Detection AR9961 Red Refine Polymer mL PA0083 Bond 3.75 mL 7.5 S21.2129 45 mL 7 PA0117 45 Containers Open Bond Detection R Intense Bond AR0833 AR0584 1 Solution Retrieval Epitope Bond Solution Dewax Bond Rack Cleaning CT Bond Beta-Catenin Oncoprotein Bcl-2 Antibody Anti-Fluorescein Antibody Anti-Biotin a ) A441 mL 7 16 PA0424 AR9640 AR9551 AR9432 CS9100 PA0204 PA0558 AR0222 PA0306 PA0346 PA0406 PA0539 S21.2011.110 S21.2001.110 OPT9719 S21.0304.110 S21.1913.110 S21.1003.110 DS9800 OP309700 S21.1971.110 ec irssesrpeettv o viaiiyi orregion. your your in consult availability Please for approval. representative regulatory Microsystems to Leica subject are catalog this in Products 14 13 13 48 15 15 45 14 17 16 16 49 49 48 48 48 48 12 48 48 0TtainCnanrInserts Container Titration 50 dispense) μL (150 Tests 200 container dispense) μL (150 Tests 200 1L kit 1 mL 30 Cycles Cleaning 15 7mL 7mL mL 15 7mL 7mL 7mL 7mL Labels/roll 3000 Pack 100 Pack 50 Tray 1 Pen 1 Tray 1 dispense) μL (150 Tests 200 or dispense) μL (100 Tests 300 container Tests/ 200 minimum Pack, 10 Pack 5 E-Cadherin Control Positive DNA DOG-1 Multi Cytokeratin 8/18 Cytokeratin 19 Cytokeratin 8 Cytokeratin 5 Cytokeratin A Chromogranin 1) (Syndecan CD138 CD79a CD61 CD56 CD45 Unit CD34 Page CD31 CD30 Code CD23 CD21 CD19 CD11c CD8 CD5 CD3 CD1a Product BRPoeP08 655mL 5.5 mL 7 46 mL 6.25 27 PB0589 45 mL 7 PA0032 mL 7 26 mL 7 PB0731 mL 7 mL 26 5.5 mL 7 25 mL 7 27 PA0918 45 25 mL 7 PA0114 24 mL PA0942 7 PA0134 24 PB0614 PA0535 mL Probe 7 EBER 23 PA0509 mL 7 Control Negative DNA PA0273 mL 7 23 Desmin 22 PA0443 mL 7 Weight) Molecular (High Cytokeratin 22 mL 7 PA0146 20 Cytokeratin 21 PA0938 mL 7 17 Cytokeratin 21 PA0555 mL 7 7 Cytokeratin 20 PA0790 mL 7 Probe CMV 20 PA0305 mL 7 CDX2 19 mL 7 PA0249 CD99 19 mL 7 PA0906 18 CD68 mL 7 PA0039 18 CD57 PA0270 17 CD45RO PA0266 CD43 PA0368 CD33 PA0271 CD30 CD25 CD22 CD20 CD15 CD10 CD7 CD4 CD2 PA0250 PA0153 PA0169 PA0171 PA0843 PA0554 PA0183 PA0168 PA0553 PA0235 PA0387 PB0682 PA0219 PA0909 PA0067 PA0799 PA0567 PA0468 PA0430 PA0088 PA0192 PA0308 PA0191 PA0042 PA0212 W P F etr blotting Western Paraffin Frozen 21 21 21 20 20 19 19 18 18 17 28 45 27 27 27 26 26 25 25 24 24 23 23 22 22 C I lwcytometry Flow Immunofluorescence 7mL 7mL 7mL 7mL 7mL 7mL 7mL 7mL 7mL 7mL 7mL mL 6.25 7mL 7mL 7mL 7mL 7mL 7mL 7mL 7mL 7mL 7mL 7mL 7mL 7mL O E te applications Other lcrnmicroscopy Electron 199 /
Product Name Index Product Name Index o ealdifraino l rdcspes ii u website: our visit please www.leica-microsystems.com products all on information detailed For 200 / Receptor Progesterone Pax-5 3/4 Oct Enolase Specific Neuron (Rabbit) Control Negative Muscle) (Smooth Chain Heavy Myosin Marker) (Rhabdomyosarcoma Myf-4 Actin Specific Muscle (MUM-1) 1 Oncogene Myeloma Multiple Protein) Repair (Mismatch MSH6 Protein) Repair (Mismatch MLH1 A Melan Marker Macrophage Unit Slides Plus Microsystems Leica Page Ki67 mL 7 Probe Kappa (alpha) Inhibin 28 Code Hormone Stimulating Follicle Human Probe 11) 6, (subtypes HPV PA0035 Protein-15 Fluid Disease Cystic Gross Glucagon Gastrin Fascin Receptor Estrogen Antigen Membrane Epithelial Product 5 rti A073 mL 7 mL mL 7 7 38 39 38 mL 7 mL 7 39 PA0057 mL PA0431 7 PA0532 37 mL 7 36 PA0161 mL 7 37 PA0371 36 mL PA0727 7 mL 7 PA0996 mL 7 34 Antigen Specific 35 Prostate PA0491 Phosphatase Alkaline Placental 33 mL 7 mL 7 Protein p53 PA0909 PA0391 mL 7 mL mL 7 5.5 33 Oct-2 34 PA0373 mL 7 200kD 32 Neurofilament 32 47 (Mouse) Control Negative PA0019 32 PA0048 Myoglobin PA0118 PA0655 PB0669 Myeloperoxidase mL 7 PA0620 (Lysozyme) Muramidase mL 7 31 Cytokeratin Multi Protein) Repair (Mismatch MSH2 30 mL 7 mL mL 6.25 7 Mesothelin PA0704 mL 7 mL 7 Tryptase 29 Cell Mast PA0291 46 31 Hormone 28 Luteinizing 30 Probe Lambda PA0238 PB0829 PA0014 Ki67 PA0449 PA0026 Insulin Hormone Growth Human Hormone Gonadotrophic Chorionic Human Probe 51) 33, 31, 18, 16, (subtypes HPV B Granzyme Protein Acidic Fibrillary Glial Galectin-3 XIIIa Factor PB0645 PA0110 PA0693 PB0780 PA0350 PA0594 PA0681 PA0420 PA0151 PA0312 PA0552 PA0934 PA0435 PA0777 PA0493 PA0226 PA0258 PA0129 PA0597 PA0610 PA0233 PA0752 S21.2113 PA0230 ec irssesrpeettv o viaiiyi orregion. your your in consult availability Please for approval. representative regulatory Microsystems to Leica subject are catalog this in Products 47 31 31 46 30 30 29 29 28 39 39 38 37 37 36 36 35 35 34 33 33 32 49 32 . mL 5.5 7mL 7mL mL 6.25 7mL 7mL 7mL 7mL 7mL 7mL 7mL 7mL 7mL 7mL 7mL 7mL 7mL 7mL 7mL 7mL 7mL 7mL slides/box 72 x Boxes 20 7mL rdc oePg Unit ZAP-70 Page Factor Willebrand von Villin Code Factor-1 Transcription Thyroid Thyroglobulin (TRAP) Phosphatase Acid Tartrate-Resistant Wash Stringency Actin Muscle Smooth S-100 Probe Control Negative RNA Phosphatase Acid Prostatic Product im'TmrP06 47mL 7 mL 7 44 mL 7 43 43 PA0562 PA0033 mL mL 7 7 PA0322 mL 7 mL 7 41 42 41 mL 7 40 PA0299 PA0776 42 mL PA0331 5.5 PA0736 mL 7 Tumor Wilms' 47 PA0339 40 Vimentin Tyrosinase PB0785 Hormone Stimulating Thyroid PA0286 Transferase Deoxynucleotidyl Terminal Synaptophysin Somatostatin Serotonin Probe Control Positive RNA 9.5 Product Gene Protein PA0400 PA0106 PA0364 PA0025 PA0093 AR0633 PA0943 PA0900 PB0809 PA0006 PA0998 W P F etr blotting Western Paraffin Frozen 43 43 42 42 41 47 41 40 47 40 44 C I lwcytometry Flow Immunofluorescence 7mL 7mL 7mL 7mL 7mL mL 6.25 7mL 7mL mL 5.5 7mL 7mL O E te applications Other lcrnmicroscopy Electron 201 /
Product Name Index Product Name Index o ealdifraino l rdcspes ii u website: our visit please www.leica-microsystems.com products all on information detailed For 202 / Oncoprotein Bcl-6 ready-to-use Bond Oncoprotein bcl-2 Oncoprotein bcl-2 Oncoprotein bcl-2 (BOB-1) Protein-1 Binding Octamer Specific Cell B System Blocking Avidin/Biotin 1 Factor Activating Protease Apoptosis Protein) Coli Polyposis (Adenomatous APC Diluent Antibody Receptor Androgen (p80) (CD246) (ALK) Kinase Lymphoma Anaplastic Unit Protein Precursor Amyloid Page Component A Amyloid Alpha-Synuclein ready-to-use Bond (SMA) Actin Muscle Smooth Alpha Code tablets 10 Alpha-Internexin Fetoprotein Alpha 176 Crystallin B Alpha Alpha-1-Antitrypsin NCL-DAB (p80) (CD246) Kinase) Lymphoma (Anaplastic ALK (Phosphorylated) Akt (APC) Protein Coli Polyposis Adenomatous Tetrahydrochloride Diaminobenzidine 3,3' Product Novocastra c- norti C--c--6 01m,01mL 0.1 mL, mL 1 1 mL 7 60 60 0.1mL mL, 1 NCL-L-Bcl-6-564 60 NCL-Bcl-3 60 mL RTU-bcl-2 1 mL 1 NCL-bcl-2 59 mL 1 59 NCL-MB2 58 NCL-L-AK2 mL mL 1 0.1 mL, 1 Oncoprotein Bcl-6 Oncoprotein NCL-AP2G Bcl-3 59 58 Oncoprotein bcl-2 NCL-CLUSTERIN mL 1 mL 1 Oncoprotein NCL-AR-318 bcl-2 mL ready-to-use 1 (BOB-1) Bond Protein-1 Binding Octamer 57 Specific Cell B 57 (MB2) Marker Cell B 57 NCL-AMP 2 Kinase NCL-APP Aurora mL 7 NCL-L-ASYN (Clusterin) mL J 1 Apolipoprotein mL 1 Gamma AP-2 mL 57 0.1 mL, mL 1 7 56 Receptor Androgen 57 mL 0.1 ready-to-use mL, Bond (p80) 1 RTU-SMA (CD246) 55 (ALK) NCL-A-CAT 56 Kinase Lymphoma Anaplastic mL 1 mL 1 NCL-SMA NCL-alpha-ACT Protein Precursor Amyloid 54 Protein 54 P Amyloid PA0963 55 NCL-CD166 Alpha-Synuclein NCL-ADENO NCL-A1ACp (SMA) Actin Muscle Smooth Alpha (SMA) Actin Muscle Smooth Alpha ready-to-use Bond Fetoprotein Alpha Alpha-Catenin Alpha-Actinin Alpha-1-Antichymotrypsin (CD166) ALCAM Adenovirus PA0943 NCL-A-INTER NCL-AFP NCL-ABCrys-512 NCL-A1Ap NCL-ALK NCL-L-Akt-Phos NCL-APC NCL-Bcl-6 PA0117 NCL-L-bcl-2 NCL-bcl-2-486 NCL-L-BOB-1 RE7170-K NCL-APAF1 NCL-APC RE7133 NCL-AR-2F12 NCL-ALK NCL-APP-228 NCL-AAC NCL-ASYN A581 mL 7 15 PA0558 mL 7 58 PA0306 ec irssesrpeettv o viaiiyi orregion. your your in consult availability Please for approval. representative regulatory Microsystems to Leica subject are catalog this in Products 57 56 56 55 55 54 54 54 60 60 60 60 59 176 59 58 181 58 58 57 57 57 7mL 1mL 0.1mL mL, 1 mL 0.1 mL, 1 1mL mL 0.1 mL, 1 mL 0.1 mL, 1 1mL L . mL 0.1 mL, 1 7mL 1mL mL 0.1 mL, 1 mL 0.1 mL, 1 2×18mL 1mL 1mL mL 500 1mL mL 0.1 mL, 1 1mL 1mL 1mL Caspase-2 (CD66e) Antigen Carcinoembryonic (CD66e) Antigen Carcinoembryonic IX Anhydrase Carbonic (CAL6) Calretinin (5A5) Calretinin ready-to-use Bond (Basic) Calponin Calpain Calpain ready-to-use Bond Calcitonin Calcitonin ready-to-use Bond Antigen) Cancer (Ovarian CA125 Antigen) Cancer (Ovarian CA125 Lewis Unit (Sialyl CA19-9 Page Lewis (Sialyl CA19-9 4 Protein Morphogenetic Bone Marker) (Myeloid BM1 Code XIIIa) (Factor XIIIa Factor Coagulation Blood Antibody Secondary Biotinylated Beta-Dystroglycan Beta-Catenin Beta-2-Microglobulin Bcl-w ready-to-use Bond Oncoprotein Bcl-6 Product c-0OcpoenNLBl1 11mL 1 61 NCL-Bcl-10 Oncoprotein Bcl-10 aps- CP2 C-P3 71m,01mL 0.1 mL, 1 67 mL 1 NCL-CPP32 mL 1 67 mL 7 mL 7 mL 7 mL 0.1 mL, 67 1 NCL-CPMm mL 1 mL, 2.5 66 67 mL 0.1 66 mL, 1 NCL-L-CEA-2 RTU-CALRETININ 66 NCL-CALRETININ 65 mL 1 66 PA0004 NCL-CALP-12A2 mL PA0346 (CPP32) 0.1 Caspase-3 mL, NCL-CALPONIN-B 1 mL 1 ready-to-use Bond (CD66e) 65 Antigen Carcinoembryonic NCL-CALMODULIN 65 (CD66e) Antigen Carcinoembryonic NCL-L-CALCITONIN 64 M Carboxypeptidase NCL-CALBINDIN ready-to-use mL Bond 7 (CAL6) Calretinin mL 1 (5A5) Calretinin mL 1 64 (5A5) Calretinin mL 1 64 (Basic) Calponin 63 RTU-CA125 Calpain mL mL 1 0.1 NCL-CA125 63 mL, 1 NCL-BrdU Calmodulin 177 Calcitonin 63 NCL-BM2 mL 1 Calbindin NCL-BIOTIN mL 1 NCL-CD22-2 Antigen) Cancer (Ovarian CA125 mL 7 61 Antigen) Cancer (Ovarian CA125 62 Lewis 62 (Sialyl CA19-9 NCL-bcl-x NCL-B-Amyloid Bromodeoxyuridine Marker) PA0083 (Myeloid BM2 ready-to-use Bond XIIIa) (Factor XIIIa Factor Coagulation Blood (CD22) BL-CAM Biotin ready-to-use Bond Beta-Catenin Amyloid Beta bcl-x a a a odready-to-use Bond ) ) ) C-A996 mL 1 64 NCL-CA19-9 NCL-BMP4 NCL-BM1 NCL-FXIIIa RE7103 NCL-b-DG NCL-B-CAT NCL-B2Mp NCL-Bcl-w PA0204 NCL-CASP-2 RTU-CEA-2 NCL-CEA-2 NCL-L-CAIX NCL-L-CALRET-566 NCL-L-CALRETININ PA0416 NCL-CALP-2C4 NCL-CALP-11B3 PA0406 NCL-CALp PA0539 NCL-L-CA125 PA0424 NCL-L-CA19-9 A496 LBond mL 7 63 PA0449 W P F etr blotting Western Paraffin Frozen 63 63 63 176 62 62 62 61 60 67 67 67 67 66 66 66 65 65 65 65 64 64 64 64 C I lwcytometry Flow Immunofluorescence L . mL 0.1 mL, 1 1mL mL 0.1 mL, 1 mL 25 mL 0.1 mL, 1 mL 0.1 mL, 1 1mL 1mL 1mL 7mL 1mL mL 0.1 mL, 1 1mL 1mL 7mL mL 2.5 1mL mL, 2.5 7mL mL 0.5 7mL 1mL 7mL 1mL 7mL O E te applications Other lcrnmicroscopy Electron 203 /
Product Name Index Product Name Index o ealdifraino l rdcspes ii u website: our visit please www.leica-microsystems.com products all on information detailed For 204 / CD13 CD11c CD10 CD10 Protein-1) (Motility-Related CD9 ready-to-use Bond CD8 CD8 ready-to-use Bond CD7 CD5 CD5 CD4 CD4 CD4 CD3 Unit CD3 Page CD3 ready-to-use Bond (LFA-2) CD2 Code (LFA-2) CD2 CD1a CD1a CD1a Caveolin-1 G Cathepsin B Cathepsin Caspase-8 Product D1 Mc1 C-D1 31mL 1 73 NCL-CD11b mL 0.1 mL, 1 mL 0.1 mL, 1 72 72 NCL-CD8-295 NCL-L-CD7-580 (Mac-1) CD11b mL CD8 0.1 mL, 1 CD7 70 NCL-CD2-271 (LFA-2) CD2 D1 odrayt-s A547 mL 7 mL 0.1 mL, 1 mL 7 mL 73 7 73 mL 1 72 73 PA0554 NCL-CD10-270 72 RTU-CD8-295 PA0270 NCL-L-CD8-295 mL 0.1 mL, 1 mL 7 71 mL 1 71 ready-to-use mL mL Bond 7 0.1 CD11c mL, 1 NCL-CD5-4C7 71 mL 1 71 ready-to-use 71 Bond CD10 PA0168 NCL-L-CD4-368 mL 7 CD10 mL NCL-CD4-1F6 70 0.1 mL, 1 CD8 PA0368 70 CD8 mL 70 NCL-CD3 7 NCL-L-CD3-565 70 PA0553 mL 0.1 mL, 1 ready-to-use Bond CD5 mL 7 RTU-CD2-271 CD5 70 ready-to-use Bond 70 CD4 mL 1 NCL-CD1a-235 mL 0.1 mL, 1 mL 0.25 CD4 mL 69 0.1 mL, CD4 1 PA0235 68 69 ready-to-use Bond CD3 NCL-CATH-L 68 CD3 NCL-CCK-8p NCL-CDm CD3 NCL-CASP-9 (LFA-2) CD2 ready-to-use Bond CD1a CD1a (Cholecystokinin) CCK-8 L Cathepsin D Cathepsin Caspase-9 NCL-CD3-PS1 PA0271 NCL-L-CD2-271 RTU-CD1a-235 NCL-L-CD1a-235 NCL-CD1a-220 NCL-L-Caveolin-1 NCL-CATH-G NCL-CATH-B NCL-CASP-8 NCL-CD13-304 NCL-L-CD11c-563 RTU-CD10-270 NCL-L-CD10-270 NCL-CD9 PA0183 NCL-CD8-4B11 PA0266 RTU-CD5-4C7 NCL-L-CD5-4C7 RTU-CD4-1F6 NCL-L-CD4-1F6 NCL-CD4-368 RTU-CD3-PS1 NCL-L-CD3-PS1 ec irssesrpeettv o viaiiyi orregion. your your in consult availability Please for approval. representative regulatory Microsystems to Leica subject are catalog this in Products 70 70 70 70 70 70 69 69 68 68 73 73 73 73 72 72 72 72 71 71 71 71 71 70 70 L . mL 0.1 mL, 1 7mL 1mL 1mL mL 0.1 mL, 1 mL 0.1 mL, 1 1mL 1mL mL 0.1 mL, 1 L . mL 0.1 mL, 1 mL 0.1 mL, 1 1mL 1mL 7mL mL 0.1 mL, 1 1mL 7mL 1mL mL 0.1 mL, 1 7mL 1mL 7mL 7mL 7mL 7mL CD38 CD37 Marker) Cell (Endothelial CD34 Marker) Cell (Endothelial CD34 ready-to-use Bond CD33 (PECAM-1) CD31 CD30 ready-to-use Bond CD30 CD30 CD30 CD27 Receptor) (Interleukin-2 CD25 mL 0.1 ready-to-use mL, Bond 1 CD23 Unit CD23 74 Page (BL-CAM) CD22 NCL-CD14-223 CD21 ready-to-use Bond CD20 Code CD20 CD20 ready-to-use Bond CD19 CD19 CD15 CD15 CD14 CD14 Product D5NLC3 81m,01mL 0.1 mL, 1 78 NCL-CD35 mL 0.1 mL, 1 77 NCL-END CD35 Marker) Cell (Endothelial CD34 mL 0.1 mL, 1 75 mL 0.1 NCL-CD21-2G9 mL, 1 mL 0.1 mL, 1 75 NCL-CD20-7D1 74 NCL-CD16 CD21 CD20 CD16 D9NLC3 81mL 1 mL 0.1 mL, 1 78 78 NCL-CD39 NCL-CD38-290 mL 0.1 mL, 1 77 mL 1 NCL-L-CD33 0.1mL mL, mL 1 7 mL 7 mL 0.1 mL, mL 1 76 7 76 77 76 NCL-L-CD30 76 77 CD39 NCL-CD30 mL 7 PA0212 CD38 PA0790 NCL-CD29 PA0250 mL 1 76 ready-to-use Bond Marker) Cell (Endothelial CD34 RTU-CD23-1B12 76 NCL-L-CD23-1B12 mL 7 CD33 mL 7 mL mL 7 ready-to-use 1 Bond (PECAM-1) CD31 mL 0.1 76 mL, 1 ready-to-use Bond 75 CD30 75 75 mL 0.1 CD30 mL, 1 NCL-L-CD20-L26 75 PA0305 CD30 PA0171 NCL-CD20-MJ1 PA0249 74 CD29 mL ready-to-use 0.1 Bond mL, Receptor) 1 (Interleukin-2 CD25 NCL-CD19-2 CD23 mL 7 74 CD23 ready-to-use Bond (BL-CAM) 74 CD22 NCL-CD15 ready-to-use Bond CD21 PA0039 CD20 CD20 CD19 ready-to-use Bond CD15 CD15 NCL-L-CD21-2G9 PA0906 RTU-CD20-L26 NCL-CD20-L26 PA0843 NCL-L-CD19-163 RTU-CD15 NCL-L-CD15 NCL-L-CD14-223 NCL-L-CD38-290 NCL-CD37 RTU-END NCL-L-END PA0555 NCL-CD31-1A10 RTU-CD30 PA0153 NCL-L-CD30-591 NCL-CD30-365 NCL-CD27 NCL-CD25-305 PA0169 NCL-CD23-1B12 NCL-CD22-2 W P F etr blotting Western Paraffin Frozen 75 75 75 75 74 74 74 74 74 78 78 77 77 77 77 76 76 76 76 76 76 76 76 75 C I lwcytometry Flow Immunofluorescence 1mL 7mL 1mL mL 0.1 mL, 1 1mL 1mL 1mL 1mL 1mL mL 0.1 mL, 1 7mL 7mL mL 0.1 mL, 1 mL 0.1 mL, 1 1mL mL 0.1 mL, 1 7mL mL 0.1 mL, 1 mL 0.1 mL, 1 7mL 7m 7mL 7mL 7mL O E te applications Other lcrnmicroscopy Electron 205 /
Product Name Index Product Name Index o ealdifraino l rdcspes ii u website: our visit please www.leica-microsystems.com products all on information detailed For 206 / CD79a CD71 CD68 CD68 CD68 Antigen) (Carcinoembryonic CD66e (CEACAM1) CD66a (P-selectin) CD62P ready-to-use Bond (GPIIIa) CD61 CD57 CD57 (NCAM) CD56 (NCAM) CD56 (ICAM-1) CD54 Unit ready-to-use Bond CD45RO Page CD45RO CD45RA Code ready-to-use Bond CD45 CD45 6 Variant CD44 3 variant CD44 CD43 CD43 (GPIb) CD42b CD40 Product D5RULAR 07mL 7 80 RTU-LCA-RP CD45 D2NLC7 31mL 1 mL 0.1 mL, 1 83 83 NCL-CD72 mL 1 mL 7 NCL-CD69 83 83 NCL-CD68-KP1 PA0273 mL 7 mL 0.1 mL, 1 mL mL 1 mL 0.1 1 mL, 1 83 82 mL 83 7 82 82 RTU-CEA-2 CD72 NCL-CD62L-489 NCL-CD63 NCL-CD61-308 NCL-CEA-2 mL 82 CD69 1 mL mL 7 7 mL 0.1 mL, 1 ready-to-use Bond CD68 mL 0.1 mL, 1 81 CD68 PA0443 81 81 81 NCL-L-CD56-1B6 mL 1 Antigen) (Carcinoembryonic CD66e 81 mL 0.1 mL, NCL-CD56-1B6 1 Antigen) RTU-UCHL1 (Carcinoembryonic CD66e PA0191 81 Marker) NCL-UCHL1 (Melanoma CD63 80 mL 1 NCL-CD45RB (L-selectin) CD62L (GPIIIa) CD61 80 mL NCL-LCA 1 ready-to-use Bond CD57 mL 0.1 NCL-L-LCA-RP mL, 1 ready-to-use Bond (NCAM) mL CD56 1 80 (NCAM) CD56 mL 7 79 NCL-CD44v5 79 (NCAM) CD56 mL 1 NCL-CD44-2 79 CD45RO NCL-L-MT1 CD45RO 79 CD45RB PA0938 NCL-CD41 CD45 CD45 5 variant CD44 (H-CAM) CD44 ready-to-use Bond CD43 CD43 (GPIIb/IIIa) CD41 NCL-L-UCHL1 NCL-B1 PA0042 NCL-LCA-RP NCL-CD44v6 NCL-CD44v3 RTU-MT1 NCL-MT1 NCL-CD42b NCL-CD40 NCL-CD79a-192 NCL-CD71-309 RTU-CD68 NCL-L-CD68 NCL-CD68 NCL-L-CEA-2 NCL-CD66a NCL-CD62P-367 PA0308 RTU-NK1 NCL-NK1 RTU-CD56-1B6 NCL-CD56-564 NCL-CD54-307 PA0146 ec irssesrpeettv o viaiiyi orregion. your your in consult availability Please for approval. representative regulatory Microsystems to Leica subject are catalog this in Products 81 80 80 80 80 80 79 79 79 79 84 83 83 83 83 83 82 82 82 82 82 81 81 81 81 1mL 1mL 1mL 1mL 1mL 7mL 1mL 1mL mL 0.1 mL, 1 L . mL 0.1 mL, 1 mL 0.1 mL, 1 7mL 1mL 0.1mL mL, 1 1mL 1mL 1mL 7mL 7mL mL 0.1 mL, 1 1mL mL 0.1 mL, 1 mL 0.1 mL, 1 7mL 7mL oto rb (Fluorescein-Conjugated) Probe Control C9 Component Complement IV Type Collagen Oncoprotein c-myc J) (Apolipoprotein Clusterin ready-to-use Bond A Chromogranin Acetyltransferase Choline 1 Kinase Checkpoint Oncoprotein c-erbB-3 (CD66a) CEACAM1 CDX2 ready-to-use Bond (p80) (ALK) Kinase) Lymphoma (Anaplastic CD246 (P-glycoprotein) CD243 mL 0.1 mL, 1 Unit (RHAMM) CD168 Page CD163 84 (MCAM) CD146 NCL-CD79a-225 CD137 Code CD123 ready-to-use Bond CD99 (Fas) CD95 CD82 CD79b ready-to-use Bond CD79a CD79a CD79a Product D9 T-D9-9 47mL 7 84 RTU-CD79a-192 CD79a olgnTp I C-OLVI9 mL 1 mL 1 92 Tests 500 92 mL 1 NCL-COLL-VII NCL-COLL-IIp 175 mL 0.1 mL, 1 91 mL mL 0.1 1 mL, 1 mL 0.25 RE7130-K 91 NCL-cJUN NCL-CHROM-430 90 92 90 mL 7 System Detection Peroxidase Concentrated NCL-cMET NCL-CCK-8p NCL-FOS mL VII 1 Type mL 89 Collagen 1 II Type Collagen mL 89 1 Receptor) Factor 88 mL Growth 0.1 (Hepatocyte mL, c-MET 1 PA0535 Oncoprotein mL c-jun 1 88 NCL-CAS NCL-CAK 88 A Chromogranin mL 0.1 mL, 1 NCL-L-DEC205 mL 1 87 (CCK-8) Cholecystokinin mL 0.1 mL, NCL-CD166 1 Oncoprotein mL mL c-fos 0.1 mL 0.1 mL, 0.1 mL, 1 88 mL, 1 1 NCL-CD151 86 Protein Susceptibility Apoptosis Cellular 86 mL 1 ready-to-use Bond 85 85 CDX2 NCL-CD134 85 NCL-ALK NCL-CD141 (CAK) NCL-L-CD99-187 kinase cdk-activating 84 NCL-CD105 NCL-CD83 (p80) (ALK) Kinase) Lymphoma (Anaplastic CD246 mL 1 NCL-CD81 (DEC-205) CD205 84 (ALCAM) CD166 NCL-L-CD79a-225 (PETA-3) CD151 (Thrombomodulin) CD141 (OX40) CD134 (Endoglin) CD105 CD99 CD83 CD81 CD79a NCL-CD146 NCL-CD137 NCL-L-CD123 PA0509 NCL-FAS-310 NCL-CD82 NCL-L-CD79b PA0192 NCL-L-CD79a-192 NCL-CONTROL NCL-CCC9 NCL-COLL-IV NCL-cMYC NCL-CLUSTERIN PA0430 NCL-ChAT NCL-Chk1 NCL-c-erbB-3 NCL-CD66a NCL-CDX2 PA0306 NCL-PGLYm NCL-CD168 NCL-CD163 W P F etr blotting Western Paraffin Frozen 87 86 86 85 85 85 84 84 84 184 93 92 92 91 91 90 90 90 89 89 88 88 88 87 C I lwcytometry Flow Immunofluorescence L . mL 0.1 mL, 1 1mL mL 0.1 mL, 1 7mL 1mL 1mL mL 0.1 mL, 1 1mL 0Tests 50 1mL 1mL mL 0.1 mL, 1 1mL 7mL 1mL 1mL 1mL 1mL mL 0.1 mL, 1 7mL 1mL 1mL mL 0.1 mL, 1 7mL O E te applications Other lcrnmicroscopy Electron 207 /
Product Name Index Product Name Index o ealdifraino l rdcspes ii u website: our visit please www.leica-microsystems.com products all on information detailed For 208 / (5/6/18) Cytokeratin 20 Cytokeratin 20 Cytokeratin 20 Cytokeratin ready-to-use Bond 19 Cytokeratin 18 Cytokeratin 17 Cytokeratin 15 Cytokeratin 14 Cytokeratin 10 Cytokeratin ready-to-use Bond 8 Cytokeratin 8 Cytokeratin ready-to-use Bond 7 Cytokeratin Unit 7 Cytokeratin Page 7 Cytokeratin 5 Cytokeratin Tests 100 5 Cytokeratin Code 189 4 Cytokeratin Cyclooxygenase-2 NCL-CD-PS E Cyclin D1 Cyclin D1 Cyclin B1 Cyclin (Caspase-3) CPP32 diphtheriae Corynebacterium Product yoeai T-K-VL9 mL 7 96 mL 1 RTU-CK7-OVTL 96 NCL-CK6 7 Cytokeratin 6 Cytokeratin yoeai 561)NLL3 91mL 1 mL 1 99 mL 1 99 mL 7 NCL-LP34 mL 1 98 mL 0.1 mL, 1 NCL-C50 98 98 NCL-CK20 98 mL 1 NCL-CK20-543 mL 0.1 mL, 1 mL PA0918 7 NCL-CK19 97 mL 0.5 97 97 mL 7 NCL-CK16 97 (5/6/18) Cytokeratin NCL-LL002 mL 1 (5/18) Cytokeratin 96 PA0114 NCL-CK13 ready-to-use Bond 20 Cytokeratin RTU-CK8-TS1 96 20 mL Cytokeratin 1 NCL-L-CK8-TS1 mL 1 20 Cytokeratin 96 19 Cytokeratin 96 NCL-L-CK7-OVTL mL 0.1 mL, ready-to-use 1 Bond 17 Cytokeratin NCL-CK7-OVTL mL 0.5 16 Cytokeratin mL 7 95 mL 1 14 Cytokeratin 95 13 Cytokeratin 95 mL 1 NCL-CK5 94 8 Cytokeratin NCL-CK1 G mL NCL-CYCLIN 7 94 8 Cytokeratin PA0468 mL 0.1 mL mL, 1 D3 1 NCL-CYCLIN 94 D1-GM 7 RTU-CYCLIN Cytokeratin 93 94 D1-GM 7 NCL-CYCLIN Cytokeratin A NCL-CYCLIN ready-to-use Bond 5 Cytokeratin 5 Cytokeratin 1 Cytokeratin G Cyclin D3 Cyclin D1 Cyclin D1 Cyclin A Cyclin RTU-CK5 NCL-L-CK5 NCL-CK4 NCL-COX-2 E NCL-CYCLIN D1 NCL-CYCLIN D1-GM NCL-L-CYCLIN B1 NCL-CYCLIN NCL-CPP32 NCL-L-LP34 RTU-CK20 NCL-L-CK20 NCL-L-CK20-561 PA0799 NCL-CK18 NCL-CK17 NCL-CK15 NCL-L-LL002 NCL-CK10 PA0567 NCL-CK8-TS1 PA0942 NCL-CK7 NCL-L-CK7-560 ec irssesrpeettv o viaiiyi orregion. your your in consult availability Please for approval. representative regulatory Microsystems to Leica subject are catalog this in Products 95 95 95 95 94 94 94 93 93 99 98 98 98 98 98 97 97 97 97 96 96 96 100 96 1mL mL 0.5 mL 0.1 mL, 1 mL 0.1 mL, 1 1mL 1mL mL 0.1 mL, 1 mL 0.1 mL, 1 1mL 7mL 1mL mL 0.1 mL, 1 7mL mL 0.1 mL, 1 mL 0.1 mL, 1 mL 0.1 mL, 1 1mL 1mL 7mL mL 0.1 mL, 1 1mL mL 0.1 mL, 1 7mL 7mL nohla elMre (CD34) Marker Cell Endothelial ready-to-use (CD34)Bond Marker Cell Endothelial Emerin E-Cadherin ready-to-use Bond E-Cadherin (C-terminus) Dystrophin (C-terminus) Dystrophin Dysferlin DOG-1 Dinitrophenyl ready-to-use Bond Desmin Desmin Protein Cancer Colorectal in Deleted Daxx Unit tests) (250 DAB Page antigen) (pp65 Cytomegalovirus antigen) (early Cytomegalovirus Code ready-to-use Bond Multi Cytokeratin, Multi Cytokeratin, (1/5/10/14) Multi Cytokeratin, ready-to-use Bond (1/5/10/14) Multi Cytokeratin, (5/6/8/18) Multi Cytokeratin, (8/18) Cytokeratin (8/18) Cytokeratin (5/6/18) Cytokeratin Product noln(D0)NLC15131m,01mL 0.1 mL mL, 0.5 1 mL 0.1 mL, 1 mL 0.1 mL, 1 103 103 103 NCL-ELASTIN 102 NCL-CD105 mL 1 NCL-END NCL-E-Cad mL 1 mL, 2.5 mL 0.1 mL, 1 102 mL 1 mL, 2.5 102 102 mL NCL-DYSB 7 mL 102 7 NCL-Hamlet-2 NCL-DYS3 mL 1 (CD34) 101 Marker Cell Endothelial 101 NCL-DYS1 RTU-DES-DERII (CD105) Endoglin 101 NCL-L-DES-DERII mL 25 Elastin mL 1 PA0219 E-Cadherin 175 100 (N-terminus) Dystrophin mL 1 (N-terminus) Dystrophin NCL-L-DEC205 RE7125 Domain) (Rod Dystrophin mL 7 101 Dysferlin mL 1 mL 1 ready-to-use Bond 99 DOG-1 NCL-DPC4 Tests 50 100 Desmin RTU-AE1/AE3 99 mL Desmin 1 NCL-CMV-LA 184 NCL-L-AE1/AE3 Protein 4 Locus mL Cancer 0.1 Pancreatic mL, in 1 Deleted 99 (CD205) NCL-CMV mL DEC-205 7 mL 0.1 mL, 1 99 Enhancer DAB mL 1 100 NCL-CK34BE12 NCL-C11 (Fluorescein-Conjugated) Probe mL Cytomegalovirus 7 99 RTU-CK5/6/8/18 antigen) (late 100 Cytomegalovirus NCL-L-CK5/6/8/18 99 Multi Cytokeratin, NCL-5D3 Multi Cytokeratin, (4/5/6/8/10/13/18) Multi PA0067 Cytokeratin, (1/5/10/14) Multi Cytokeratin, (5/6/8/18) Multi Cytokeratin, (5/6/8/18) Multi Cytokeratin, ready-to-use Bond (8/18) Cytokeratin (8/18) Cytokeratin NCL-CMVpp65 NCL-CMV-EA PA0909 NCL-AE1/AE3 RTU-CK34BE12 PA0134 NCL-CK5/6/8/18 RTU-5D3 NCL-L-5D3 RTU-LP34 NCL-L-END PA0212 NCL-EMERIN RTU-E-Cad PA0387 NCL-DYSA NCL-DYS2 NCL-Hamlet NCL-L-DOG-1 NCL-DNP PA0032 NCL-DES-DERII NCL-DCC NCL-DAXX RE7190-K W P F etr blotting Western Paraffin Frozen 100 100 99 99 99 99 100 99 99 99 103 103 103 102 102 102 102 102 101 177 101 101 101 100 176 C I lwcytometry Flow Immunofluorescence L . mL 0.1 mL, 1 1mL 7mL mL 0.1 mL, 1 7mL 7mL mL 0.1 mL, 1 7mL 1mL 7mL 1mL 7mL mL 0.1 mL, 1 7mL 7mL 1mL mL 1 mL, 2.5 mL 0.1 mL, 1 mL 0.1 mL, 1 1mL 7mL mL 0.1 mL, 1 1mL 1mL Tests 250 O E te applications Other lcrnmicroscopy Electron 209 /
Product Name Index Product Name Index o ealdifraino l rdcspes ii u website: our visit please www.leica-microsystems.com products all on information detailed For 210 / Synthetase Folylpolyglutamate Filamin Fibronectin Ligand Fas Fascin Phosphatase-1 Fas-Associated ready-to-use Bond XIIIa) Factor Coagulation (Blood XIIIa Factor Factor) Willebrand (von Antigen VIII-Related Factor (Drosophila)) 2 Homolog Zeste of (Enhancer EZH2 Oncoprotein Ets-1 Receptor Estrogen Receptor Estrogen Receptor Estrogen Unit 2) antigen (nuclear virus Epstein-Barr Protein 3 Page Gene virus-Induced Epstein-Barr Kit ISH Probe virus mL Epstein-Barr 7 Concentrate) (x10 pH9 Solution Retrieval Epitope Code Concentrate) 103 (x10 pH6 Solution Retrieval Epitope Antigen Specific Epithelial RTU-END Antigen Epithelial-Related ready-to-use Bond Antigen Membrane Epithelial Antigen Membrane Epithelial Envoplakin (ETA) Receptor Endothelin-1 (CD34) Marker Cell Endothelial Product srgnRcpo bt)NLE-ea181mL 1 108 NCL-ER-beta (beta) Receptor Estrogen L 1 181 RE7113 Concentrate) (x10 pH6 Solution Retrieval Epitope iagi C-IAGI 1 mL 1 mL 0.1 mL, 1 111 112 NCL-FILAGGRIN mL 1 mL 7 mL NCL-L-FRalpha 0.5 110 110 111 NCL-FAS-310 PA0420 NCL-1G9 mL 1 109 Alpha mL Receptor 0.1 Folate mL, 1 Filaggrin mL 1 NCL-FADD 109 protein) mL (capsid 0.1 Calicivirus mL, Feline 1 mL 2 108 mL ready-to-use 7 Bond Fascin mL 2 NCL-FXIIIa 109 (CD95) Fas 106 NCL-EAAT2 106 106 Protein Domain NCL-L-ER-6F11/2 Death Fas-Associated mL 0.1 mL, 1 NCL-vWF NCL-ER-6F11/2 XIIIa) Factor Coagulation (Blood XIIIa Factor PA0151 Factor) 106 Willebrand (von Antigen VIII-Related Factor mL NCL-EBV-CS1-4 1 2 Transporter Acid Amino Excitatory mL 0.1 mL, 1 105 Tests Tests,10 50 L ready-to-use 1 Bond Receptor Estrogen mL NCL-EADE31 7 105 Receptor Estrogen 184 181 mL 1 mL Receptor 100 Estrogen 105 NCL-ESA (LMP-1) virus Epstein-Barr NCL-EBV 105 182 RE7116 diffuse) mL antigen 1 (early RTU-EMA virus Epstein-Barr (Fluorescein-Conjugated) Probe NCL-L-EMA virus Epstein-Barr 104 RE7160-K Concentrate) (x10 pH8 Solution Retrieval Epitope NCL-ENTERO Antigen Specific Epithelial Antigen Membrane Epithelial Antigen Membrane Epithelial (IHC) K Proteinase Enzyme (unconjugated) Enterovirus NCL-EBV-K RE7119 RE7114 RTU-ESA NCL-MOC-31 PA0035 NCL-EMA NCL-ENVO NCL-L-ETA NCL-L-FPGS NCL-FIL NCL-FIB NCL-FAS-L NCL-FASCIN NCL-FAP-1 PA0449 NCL-L-vWF NCL-L-EZH2 NCL-ETS-1 RTU-ER-6F11 NCL-ER-6F11 NCL-L-ER-6F11 NCL-EBV-PE2 NCL-EBI-3 ec irssesrpeettv o viaiiyi orregion. your your in consult availability Please for approval. representative regulatory Microsystems to Leica subject are catalog this in Products 184 182 181 105 105 105 105 104 103 112 111 111 110 110 110 109 109 109 108 106 106 106 106 106 0Tests 50 1L mL 500 1mL 7mL mL 0.1 mL, 1 1mL mL 0.1 mL, 1 L . mL 0.1 mL, 1 1mL mL 0.1 mL, 1 1mL mL 0.1 mL, 1 1mL 7mL 1mL mL 0.1 mL, 1 mL 0.1 mL, 1 0.1mL mL. 1 1mL mL 0.1 mL, 1 1mL 7mL 7mL G-51(ua ati Mucin) Gastric (Human HGM-45M1 Hormone) Growth (Human HGH 1) (type virus simplex Herpes Antigen Specific Hepatocyte (NS3) virus C Hepatitis pylori Helicobacter 105 Protein Shock Heat 70 Protein Shock Heat (CD44) H-CAM Protein-15 Fluid Disease Cystic Gross Protein-15 Fluid Disease Cystic Gross ready-to-use Bond B Granzyme B Granzyme mL 0.1 mL, 1 Unit (CD61) GPIIIa Page 112 (CD42b) GPIb Immunoglobulin Peroxidase-Conjugated Anti-Mouse Goat NCL-GAL1 (mu) S-Transferase Glutathione Code Receptor Glucocorticoid Glucagon Protein Acidic Fibrillary Glial Geminin Gastrin Gamma-Catenin Galectin-3 Galectin-1 Product eeui C-EE 2 mL 1 mL 1 120 mL 25 Tests 50 120 mL NCL-HEREG 7 175 184 NCL-HISTONE-513 NCL-HSV-2 120 RE7107 mL 1 mL 0.1 mL, 1 mL 1 PA0704 117 119 (Fluorescein-Conjugated) Probe Histone 117 mL 7 ready-to-use Bond NCL-HSP90 Hormone) Growth (Human HGH NCL-cMET 2) mL NCL-HSP27 (type 1 virus simplex Herpes 116 mL Heregulin 1 RTU-GRAN-B 116 mL 1 mL (c-MET) 1 Receptor Factor mL Growth 7 Hepatocyte NCL-L-GRAN-B 116 Hematoxylin 116 NCL-L-Granulysin 115 116 pylori NCL-L-GCDFP15 Helicobacter 90 Protein Shock Heat NCL-CD41 27 PA0350 Protein Shock Heat mL 0.1 mL, 1 ready-to-use Bond Protein-15 Fluid Disease Cystic mL Gross mL 7 1 mL 0.1 mL, 1 Protein-15 Fluid Disease Cystic mL Gross 115 1 mL 1 114 115 B Granzyme NCL-GSTpi-438 114 115 mL 7 B Granzyme 113 NCL-GnRHR mL NCL-GSTal-436 7 PA0594 Granulysin 113 NCL-GI NCL-GAP43 (CD41) GPIIb/IIIa mL 7 114 Receptor Hormone Gonadotropin-Releasing PA0681 112 (pi) S-Transferase Glutathione PA0026 (alpha) S-Transferase Glutathione ready-to-use Bond PA0238 Glucagon ready-to-use Bond Protein Acidic Fibrillary Glial intestinalis Giardia ready-to-use Bond Gastrin GAP43 ready-to-use Bond Galectin-3 NCL-GAMP NCL-GSTmu-437 NCL-GCR NCL-GLUCp NCL-GFAP-GA5 NCL-L-Geminin NCL-GASp NCL-G-CAT NCL-GAL3 NCL-HGM-45M1 NCL-HGH NCL-HSV-1 NCL-HSA NCL-HCV-NS3 NCL-L-Hpylori NCL-HSP105 NCL-HSP70 NCL-CD44-2 RTU-GCDFP15 NCL-GCDFP15 PA0291 NCL-GRAN-B NCL-CD61-308 NCL-CD42b C-P 1 mL 1 118 NCL-HPp W P F etr blotting Western Paraffin Frozen 177 115 114 114 114 113 113 113 112 120 120 120 119 118 118 117 117 116 116 116 116 116 115 115 C I lwcytometry Flow Immunofluorescence 1mL 1mL 1mL mL 0.5 mL 0.1 mL, 1 1mL mL 0.5 1mL mL 0.1 mL, 1 L . mL 0.1 mL, 1 mL 0.25 1mL mL 0.1 mL, 1 mL 0.1 mL, 1 mL 0.1 mL, 1 1mL 1mL mL 0.1 mL, 1 7mL mL 0.1 mL, 1 7mL mL 0.1 mL, 1 mL 0.1 mL, 1 1mL O E te applications Other lcrnmicroscopy Electron 211 /
Product Name Index Product Name Index o ealdifraino l rdcspes ii u website: our visit please www.leica-microsystems.com products all on information detailed For 212 / Chain Light Lambda Protein) (p57 Kip2 ready-to-use Bond Antigen Ki67 Antigen Ki67 Antigen Ki67 Chain Light Kappa (Fluorescein-Conjugated) Probes Kappa/Lambda 6 Interleukin (CD25) Receptor Interleukin-2 Insulin M immunoglobulin G immunoglobulin D immunoglobulin Unit A immunoglobulin Page Diluent IHC Protein 2 Gene Inducible Hypoxia antigen) VIII-related (Factor Code Factor Willebrand von Human Polypeptide Spasmolytic Human (1/2/3) Defensins Neutrophil Human (Fluorescein-Conjugated) Probe 8) (type Herpesvirus Human (HGH) Hormone Growth Human ready-to-use Bond (beta) Gonadotrophin Chorionic Human (alpha) Gonadotrophin Chorionic Human Antigen (DR) II Class HLA Product abaLgtCanNLLM 2 mL 1 mL 127 0.1 mL, 1 mL 7 mL NCL-LAMp 7 127 mL 0.1 mL, 1 126 NCL-L-LAM-578 126 mL 0.1 mL 126 RTU-Ki67-MM1 mL, 0.1 1 mL, 1 mL 0.1 NCL-Ki67-MM1 mL, 1 126 126 PA0230 126 NCL-L-KAP-581 mL 0.1 NCL-INV NCL-KAP-L1C1 mL, 1 mL 7 Chain Light Lambda Chain mL 125 Light 0.1 Lambda mL, 1 125 mL ready-to-use 0.1 Bond mL NCL-L-InhibinA mL, Antigen 7 1 Ki67 124 mL 0.1 mL, Antigen 1 Ki67 PA0620 125 124 mL 0.1 mL, Antigen 1 Ki67 NCL-L-IgM 124 mL Chain 0.1 Light mL, Kappa 1 NCL-L-IgG 123 PA0368 Chain Light Kappa NCL-L-IgD 123 Involucrin NCL-L-IgA ready-to-use Bond NCL-CD54-307 (CD25) Receptor Interleukin-2 mL 1 ready-to-use Bond Insulin InhibinA 122 M immunoglobulin NCL-SECURIN G Immunoglobulin D Immunoglobulin mL 1 A Immunoglobulin mL mL 7 0.1 mL mL, 0.1 1 123 mL, (CD54) 1 ICAM-1 mL 1 mL 1 ready-to-use antigen) Bond VIII-related (Factor 121 Factor Willebrand 121 von Human NCL-L-vWF 121 120 antigen) VIII-related 121 (Factor Factor NCL-HGM-45M1 Willebrand von Human NCL-HHV8-LNA NCL-HMB45 Securin PA0704 Human NCL-HCGp antigen) nuclear (latent 8) (type Herpesvirus Human ready-to-use Bond (HGH) Hormone Growth Human (HGM-45M1) Mucin Gastric Human (beta) Gonadotrophin Chorionic Human Marker) (Melanoma HMB45 NCL-L-HIG2 NCL-vWF NCL-HSP NCL-DEFENSIN NCL-HHV8 NCL-HGH PA0014 NCL-HCG-alpha NCL-LN3 NCL-LAM NCL-p57 PA0118 NCL-L-Ki67-MM1 NCL-Ki67p NCL-KAP NCL-KAP/LAM NCL-L-IL6 NCL-CD25-305 NCL-INSULIN NCL-IgMp NCL-IgGp NCL-IgDp NCL-IgAp RE7133 A40137mL 7 123 PA0400 ec irssesrpeettv o viaiiyi orregion. your your in consult availability Please for approval. representative regulatory Microsystems to Leica subject are catalog this in Products 123 123 122 122 185 121 121 121 120 127 126 126 126 126 126 185 125 125 125 124 124 124 124 181 L . mL 0.1 mL, 1 mL 0.1 mL, 1 1mL 1mL Tests 10 Tests, 50 mL 0.25 7mL 1mL mL 0.1 mL, 1 L 0.1mL mL, 1 mL 0.1 mL, 1 7mL 1mL mL 0.2 1mL Tests 25 × 2 mL 0.1 mL, 1 mL 0.1 mL, 1 mL 0.1 mL, 1 1mL 1mL 1mL 1mL mL 500 ,2x5T ests imthRpi rti MH)Bn ready-to-use Bond (MSH6) Protein Repair Mismatch ready-to-use Bond (MSH2) Protein Repair Mismatch 7 Protein Maintenance Minichromosome 5 Protein Maintenance Minichromosome 2 Protein Maintenance Minichromosome (MITF) Factor Transcription Microphthalmia Mesothelin Chain 2 Alpha Laminin Merosin (CD63) Marker Melanoma ready-to-use Bond A Melan A Melan Mcl-1 Marker) Cell (B MB2 10 Metalloproteinase Matrix Unit 2 Metalloproteinase Matrix Page Tryptase Cell Mast MAGE-1 Code (LN-5) Marker Macrophage ready-to-use Bond (Muramidase) Lysozyme kD) (110 Protein Resistance-Related Lung virus) (Epstein-Barr LMP-1 Cells T of Activation for Linker (CD2) LFA-2 Langerin A/C Lamin Product imthRpi rti MH)NLLMH 3 L . mL 0.1 mL, 1 mL 1 135 134 mL NCL-L-MSH6 1 mL 0.1 mL, 1 mL 7 NCL-MSH2 mL 1 mL 7 133 134 mL mL 1 0.1 mL, 1 133 133 NCL-L-MESO 132 0.1mL mL, NCL-L-MCM6 1 133 mL 132 0.1 mL, NCL-MCM3 1 (MSH6) RTU-MelanA Protein Repair Mismatch PA0373 132 mL 0.1 mL, 1 (MSH2) NCL-MelanA Protein Repair Mismatch NCL-MITF 132 NCL-HMB45 6 Protein Maintenance Minichromosome 131 3 NCL-MDM2 Protein Maintenance Minichromosome mL mL (MITF) 0.1 1 Factor mL, NCL-CD146 Transcription 1 Microphthalmia ready-to-use mL Bond 0.1 Mesothelin mL, 1 131 130 Mesothelin Bond mL 7 mL 1 131 NCL-MASPIN NCL-MMP19 (HMB45) Marker Melanoma NCL-MMP9-439 130 A Melan 129 mL 1 A Melan mL 1 mL 1 NCL-CD11b 129 Protein MDM2 PA0019 mL 7 (CD146) 129 MCAM mL mL 7 NCL-MAC387 0.1 128 mL, 1 19 Metalloproteinase 128 Matrix mL 0.5 NCL-MURAM NCL-CD62L-489 128 9 Metalloproteinase 128 Matrix RTU-CD2-271 127 ready-to-use Bond Tryptase Cell Mast NCL-CD2-271 RTU-LAT NCL-LAMININ Maspin (MAC387) Marker Macrophage (CD11b) Mac-1 (Muramidase) Lysozyme (CD62L) L-selectin Cells T of Activation for Linker (CD2) LFA-2 (CD2) LFA-2 Laminin NCL-MCTRYP-428 NCL-MAGE-1 NCL-LN5 PA0391 NCL-LRRP NCL-EBV-CS1-4 NCL-L-LAT NCL-L-CD2-271 NCL-LANGERIN NCL-LAM-A/C PA0597 PA0048 NCL-MCM7 NCL-MCM5 NCL-MCM2 NCL-L-MITF NCL-MESO NCL-MEROSIN NCL-CD63 PA0233 NCL-L-MelanA NCL-Mcl-1 NCL-MB2 NCL-MMP10 NCL-MMP2-507 W P F etr blotting Western Paraffin Frozen 130 130 129 129 128 128 128 128 127 127 135 134 133 133 133 133 133 132 132 132 132 131 131 131 131 C I lwcytometry Flow Immunofluorescence L . mL 0.1 mL, 1 1mL 1mL 7mL 1mL mL 0.1 mL, 1 1mL 1mL mL 0.1 mL, 1 mL 0.1 mL, 1 7mL 7mL 1mL 1mL mL 0.1 mL, 1 mL 0.1 mL, 1 1mL 1mL mL 0.1 mL, 1 7mL 1mL 1mL 1mL 1mL mL 0.1 mL, 1 O E te applications Other lcrnmicroscopy Electron 213 /
Product Name Index Product Name Index o ealdifraino l rdcspes ii u website: our visit please www.leica-microsystems.com products all on information detailed For 214 / ready-to-use Bond Antibodies Chain Heavy Myosin (slow) Chain Heavy Myosin (fast) Chain Heavy Myosin ready-to-use Bond Myoglobin (Myf-4) Myogenin ready-to-use Bond (Myf-4) Myogenin Myeloperoxidase (BM1) Marker Myeloid tuberculosis Mycobacterium ready-to-use Bond Actin Specific Muscle Actin Specific Muscle ready-to-use Bond (Lysozyme) Muramidase (MUM-1) 1 Oncogene Myeloma Multiple Unit 1 Protein Resistance-associated Multidrug Page ready-to-use Bond Multi-Cytokeratin Multi-Cytokeratin mL 0.1 mL, 1 (5/6/8/18) Multi-Cytokeratin Code (4/5/6/8/10/13/18) Multi-Cytokeratin 134 1/5/10/14 Multi-Cytokeratin NCL-L-MLH1 glycoprotein muc-6 glycoprotein muc-2 glycoprotein core muc-1 4 Kinase Kinase Protein Mitogen-Activated ready-to-use Bond (MLH1) Protein Repair Mismatch (MLH1) Protein Repair Mismatch Product ytlnNLMOII 4 mL 1 140 NCL-MYOTILIN mL 0.1 mL mL, 0.1 1 mL, mL 1 0.1 mL, 1 mL 1 140 mL 139 0.1 mL, 140 1 NCL-MYOGLOBIN 139 NCL-MHC-Sm NCL-MHCn mL 140 1 mL 0.1 mL, mL 1 NCL-L-Myf-4 7 139 NCL-MHCd 138 139 Myotilin muscle) (smooth Tests Chain 100 NCL-BM2 Heavy Myosin mL 1 NCL-MBP (neonatal) Chain Heavy Myosin PA0491 mL 189 1 (developmental) Chain Heavy 138 Myosin NCL-L-MSA-594 NCL-MT-PS 138 Myoglobin mL 7 (Myf-4) Myogenin NCL-MURAM mL 1 ready-to-use Bond 136 Myeloperoxidase mL 1 (BM2) Marker Myeloid RTU-AE1/AE3 136 mL 7 Protein Basic Myelin mL 7 137 NCL-L-AE1/AE3 tuberculosis 136 Mycobacterium mL 1 137 Actin NCL-MRP3 Specific Muscle RTU-CK5/6/8/18 mL (Lysozyme) 137 1 Muramidase mL 0.1 mL, PA0129 1 mL 1 ready-to-use mL Bond 7 (MUM-1) NCL-L-CK5/6/8/18 1 Oncogene Myeloma Multiple 137 3 Protein 136 Resistance-associated 136 Multidrug 137 NCL-CK34BE12 mL 1 NCL-MUC-5AC Multi-Cytokeratin NCL-MUC-1 Multi-Cytokeratin mL 0.1 136 mL, PA0134 1 (5/6/8/18) Multi-Cytokeratin (5/6/8/18) Multi-Cytokeratin 135 NCL-CD9 ready-to-use Bond 1/5/10/14 Multi-Cytokeratin NCL-L-PMS2 1/5/10/14 Multi-Cytokeratin glycoprotein muc-5AC glycoprotein muc-1 (CD9) Protein-1 Motility-Related (PMS2) Protein Repair Mismatch NCL-AE1/AE3 NCL-CK5/6/8/18 NCL-C11 RTU-CK34BE12 NCL-MUC-6 NCL-MUC-2 NCL-MUC-1-CORE NCL-MKK4 PA0610 PA0493 NCL-MHCs NCL-MHCf PA0727 NCL-Myf-4 PA0226 NCL-MYELO NCL-BM1 NCL-MT PA0258 NCL-MSA PA0391 NCL-L-MUM1 NCL-MRP1 PA0909 ec irssesrpeettv o viaiiyi orregion. your your in consult availability Please for approval. representative regulatory Microsystems to Leica subject are catalog this in Products 136 137 137 137 136 136 136 135 134 140 140 140 139 139 139 139 139 138 138 138 138 136 137 136 1mL mL 0.1 mL, 1 1mL 7mL mL 0.1 mL, 1 mL 0.1 mL, 1 1mL 1mL 7mL 7mL mL 0.1 mL, 1 mL 0.1 mL, 1 7mL mL 0.1 mL, 1 7mL mL 0.1 mL. 1 1mL 1mL 7mL 1mL 7mL mL 0.1 mL, 1 mL 0.1 mL, 1 7mL 2 Protein p27 Protein) (WAF1 p21 (CA125) Antigen Cancer Ovarian (CA125) Antigen Cancer Ovarian Osteopontin ready-to-use Bond Oct-3/4 Oct-2 kD) (88 Nucleoporin NovoPen System Detection Polymer Novolink System Detection Polymer Min Novolink Polymer Max Novolink (Polymer) DAB Novolink Serum Horse Normal Unit Protein nm23 Page Synthase-1 Oxide Nitric Enolase Specific Neuron Code Enolase Specific Neuron ready-to-use Bond kD 200 Neurofilament kD 200 Neurofilament kD 68 Neurofilament (gp75) Receptor Factor Growth Nerve (CD56) NCAM (CD56) NCAM N-Cadherin Product 2 WF rti)NLWF1141m,01mL 0.1 mL, 1 mL 1 144 144 NCL-WAF-1 NCL-CD134 mL mL 0.1 0.1 mL, mL, 1 1 mL 1 143 144 144 Bond mL 7 NCL-O-NECTIN NCL-L-Oct3/4 mL NCL-L-CA125 7 mL 0.1 mL, 143 1 Tests 250 144 143 Protein) (WAF1 p21 PA0532 Tests 174 500 NCL-HCV-NS3 (CD134) OX40 PA0539 (CA125) Antigen 174 Cancer Ovarian RE7200-K Tests 1,250 ready-to-use Bond (CA125) Antigen Cancer Tests mL Ovarian 1,250 10 mL RE7150-K 10 174 Osteonectin 174 181 mL 1 Oct-3/4 mL 181 7 NCL-R-SERUM RE7270-K ready-to-use Bond Oct-2 NCL-G-SERUM 142 RE7280-K virus) mL C 1 (Hepatitis 142 NS3 System Detection Polymer Novolink NCL-NOS-3 mL 1 mL 142 1 Polymer Novolink mL 1 PA0435 System Detection 142 Polymer Max Novolink 142 NCL-NSE2 mL 142 (Polymer) 1 DAB mL Max 7 Novolink NCL-L-NSE2 mL NCL-NF200 0.1 mL, Serum 1 Rabbit Normal mL 0.1 mL, 141 1 141 NCL-NF68 Serum Goat Normal 141 RTU-CD56-1B6 Synthase-3 Oxide 140 Nitric NCL-NB84 NCL-CD56-564 Enolase Specific NCL-L-NapsinA Neuron Enolase Specific Neuron Enolase Specific Neuron kD 200 Neurofilament kD 68 Neurofilament Marker Neuroblastoma (CD56) NCAM (CD56) NCAM A Napsin NCL-NOS-1 RTU-NSE2 NCL-NSE-435 PA0371 NCL-NF200-N52 NCL-NF68-DA2 NCL-NGFR NCL-L-CD56-1B6 NCL-CD56-1B6 NCL-L-N-Cad NCL-p27 NCL-L-WAF-1 RTU-CA125 NCL-CA125 NCL-O-PONTIN PA0934 NCL-OCT2 NCL-Nup88 NCL-PEN RE7140-K RE7290-K RE7260-K RE7230-K NCL-H-SERUM NCL-nm23 W P F etr blotting Western Paraffin Frozen 142 142 142 142 142 142 141 141 141 141 144 144 144 144 144 143 143 143 177 174 174 174 174 181 143 C I lwcytometry Flow Immunofluorescence 1mL 7mL 1mL 7mL 1mL 1mL mL 0.1 mL, 1 1mL mL 0.1 mL, 1 mL 0.1 mL, 1 L . mL 0.1 mL, 1 1mL 7mL 1mL mL 0.1 mL, 1 7mL mL 0.1 mL, 1 1mL Pen 1 Tests 250 Tests 50 Tests 1,250 Tests 250 mL 10 1mL O E te applications Other lcrnmicroscopy Electron 215 /
Product Name Index Product Name Index o ealdifraino l rdcspes ii u website: our visit please www.leica-microsystems.com products all on information detailed For 216 / Receptor Progesterone Receptor Progesterone viruses) (JC/BK Polyomavirus Pneumolysin 1) (Type Inhibitor Activator Plasminogen Phosphatase Alkaline Placental Phosphatase Alkaline Placental (CD243) P-glycoprotein System Detection Peroxidase Block Peroxidase Perforin ready-to-use Bond (CD31) PECAM-1 P-Cadherin Pax-5 Unit (Alpha) Parvalbumin Page mL 1 18) (type Papillomavirus ready-to-use Bond (DO-7) Protein p53 146 (CD246) Code (ALK) Kinase) Lymphoma (Anaplastic p80 NCL-L-p53-DO7 (alpha) Protein p73 (Phosphospecific) Protein p53 Protein p53 (DO-1) Protein p53 (BP53-12) Protein p53 (DO-7) Protein p53 (DO-7) Protein p53 Product rabmnNLPE 5 mL 1 151 mL 2 NCL-PREp 152 mL 1 NCL-L-PGR-312/2 150 Tests 50 mL 7 mL mL 0.1 1 mL, 1 NCL-PC 185 150 mL 1 150 151 NCL-POLYd(T) mL 1 Tests 250 149 NCL-CD151 Receptor NCL-PDEGF Progesterone mL 1 PA0161 mL 0.1 mL, 150 1 Prealbumin 175 NCL-PERIPH NCL-L-PLAP-8A9 149 (Fluorescein-Conjugated) Probe d(T) Poly 149 mL 7 mL 0.1 mL, Factor 1 Growth Endothelial RE7110-K Platelet-Derived NCL-CD31-1A10 NCL-P-Cad Marker Cell Plasma 148 148 mL 0.1 Phosphatase mL, Alkaline 1 Placental NCL-PARVO Phosphatase Alkaline Placental PA0552 mL 148 0.2 mL (CD151) 0.1 PETA-3 mL, 2 mL 0.1 NCL-PTH-488 System mL, Detection 1 Peroxidase 146 147 Peripherin NCL-p53-CM5p 147 (CD31) NCL-HPV-4C4 PECAM-1 mL mL 0.1 7 mL, 1 P-Cadherin NCL-p57 mL ready-to-use 0.2 Bond Pax-5 145 146 mL 0.1 mL, 1 B19 Parvovirus 145 NCL-p53-DO7 Hormone Parathyroid 145 PA0057 NCL-p53-CM1 11,18) 6, (types Papillomavirus NCL-p53-1801 (CM5) Protein p53 ready-to-use Bond (CD246) (ALK) Kinase) Lymphoma (Anaplastic p80 (Kip2) Protein p57 ready-to-use Bond Protein p53 (DO-7) Protein p53 (CM1) Protein p53 (1801) Protein p53 NCL-HPV18 PA0057 NCL-ALK NCL-p73 NCL-p53-PHOS NCL-p53-505 NCL-p53-DO1 NCL-p53-BP RTU-p53-DO7 NCL-PGR-312 NCL-L-PGR-312 NCL-JCBK NCL-SPNm NCL-PAI-1 RTU-PLAP-8A9 NCL-PLAP-8A9 NCL-PGLYm RE7120-K RE7101 NCL-PERFORIN PA0250 NCL-L-P-Cad NCL-L-PAX-5 NCL-PARVALBUMIN A36177mL 7 147 PA0306 ec irssesrpeettv o viaiiyi orregion. your your in consult availability Please for approval. representative regulatory Microsystems to Leica subject are catalog this in Products 147 146 147 147 146 145 146 145 146 152 152 151 151 150 150 150 150 175 174 149 149 149 148 148 L . mL 0.1 mL, 2 7mL mL 0.1 mL, 1 1mL 1mL 1mL 1mL 1mL 7mL L . mL 0.1 mL, 1 1mL mL 0.1 mL, 1 1mL mL 0.1 mL, 1 7mL mL 0.1 mL, 1 1mL Tests 500 mL 25 mL 0.1 mL, 1 7mL 1mL 1mL 1mL illLewis Sialyl ready-to-use Bond Serotonin Ca Reticulum Endoplasmic or Sarcoplasmic Delta Sarcoglycan, Beta Sarcoglycan, (adhalin) Alpha Sarcoglycan, S-100 S-100 S-100 Protein Gene Retinoblastoma Protein Gene Retinoblastoma virus syncytial Respiratory PTEN Unit (CD243) P-glycoprotein Page K Proteinase 9.5 Product Gene mL Protein 2 Block Protein Code 152 ready-to-use Bond Phosphatase Acid Prostatic NCL-PGR-312/2 1 Protein Overexpressed Tumor Prostate Antigen Specific Prostate Antigen Specific Prostate Antigen Nuclear Cell Proliferating Proinsulin ready-to-use Bond Receptor Progesterone Receptor Progesterone Product SRA Antibodies (SERCA) eooi C-EOp1805mL 0.5 158 NCL-SEROTp mL 0.1 mL, 1 mL 1 157 mL 0.1 mL, 1 157 mL 1 NCL-g-SARC mL 1 NCL-L-b-SARC mL 7 157 157 157 mL 1 157 NCL-L-S100-A7 NCL-S100 NCL-L-a-SARC mL 0.1 Lewis mL, Sialyl 1 156 PA0900 Serotonin mL 0.1 mL, 1 156 NCL-CD168 mL 0.5 Gamma Sarcoglycan, mL 0.1 mL, 1 156 mL 1 Beta NCL-RET Sarcoglycan, 154 (adhalin) 155 Alpha NCL-RB-358 Sarcoglycan, 155 mL (Psoriasin) 0.1 S100A7 mL, 1 NCL-pS2 NCL-CD62P-367 ready-to-use NCL-RCC Bond S-100 mL 1 154 S-100 (CD168) RHAMM mL 154 NCL-PGP9.5 7 mL 1 Protein Gene Retinoblastoma 154 Oncoprotein ret 154 NCL-PIP Marker Carcinoma Cell Renal mL 1 NCL-L-PAP (CD62P) mL PA0286 P-selectin 1 mL 7 153 Protein pS2 mL 7 mL 1 153 9.5 Product Gene 153 Protein NCL-L-PSMA NCL-L-PSA-28A4 ready-to-use Bond 9.5 152 Product Gene Protein 152 Peptide Inhibin Prostatic RTU-PGR-312 PA0431 NCL-L-PCNA Phosphatase Acid Prostatic Antigen Membrane Specific Prostate ready-to-use Bond Antigen Specific Prostate Antigen Specific Prostate Antigen Nuclear Cell Proliferating Receptor Progesterone a a (CA19-9) (CA19-9) 2+ ATPase C--A99181mL 1 158 NCL-L-CA19-9 NCL-L-PGP9.5 RE7102 PA0006 NCL-PTOV1 RTU-PSA-28A4 NCL-PSA-431 NCL-PCNA NCL-PROIN-1G4 PA0312 NCL-CA19-9 PA0736 NCL-SERCA2 NCL-d-SARC NCL-b-SARC NCL-a-SARC RTU-S100p NCL-S100p NCL-L-S100p NCL-RB NCL-L-RB-358 NCL-RSV3 NCL-PTEN NCL-PGLYm NCL-PK W P F etr blotting Western Paraffin Frozen 154 174 154 153 153 153 152 152 152 158 158 158 157 157 157 157 157 157 156 156 155 155 150 185 C I lwcytometry Flow Immunofluorescence 1mL mL 25 7mL 1mL 7mL mL 0.1 mL, 1 mL 0.1 mL, 1 1mL 7mL 1mL 7mL mL 0.5 1mL mL 0.1 mL, 1 mL 0.1 mL, 1 7mL 1mL 1mL 1mL 1mL 1mL mL 0.1 mL, 1 1mL μg 500 O E te applications Other lcrnmicroscopy Electron 217 /
Product Name Index Product Name Index o ealdifraino l rdcspes ii u website: our visit please www.leica-microsystems.com products all on information detailed For 218 / II Beta Tubulin C Troponin Beta Factor Growth Transforming Alpha II Topoisomerase Protein Domain Death TNFR1-Associated 3 Metalloproteinase Matrix of Inhibitor Tissue ready-to-use Bond Factor-1 Transcription Thyroid Factor-1 Transcription Thyroid ready-to-use Bond Hormone Stimulating Thyroid Peroxidase Thyroid Thyroglobulin (CD141) Thrombomodulin ready-to-use Bond Transferase Deoxynucleotidyl Terminal Transferase Deoxynucleotidyl Terminal Unit mL 1 Tau Page 158 (TRAP) Phosphatase Acid Tartrate-Resistant NCL-L-SM22a Synaptophysin Code Synaptophysin 2 Protein Vesicle Synaptic B Protein Precursor Surfactant Streptavidin-HRP use) human for (recommended Spectrin Actin) Muscle Smooth (Alpha SMA ready-to-use Bond Actin) Muscle Smooth (Alpha SMA alpha SM22 Product uei C-UEI 6 mL 1 166 NCL-TUBERIN mL 1 165 mL 0.1 I mL, NCL-TOPO 1 166 mL 1 mL 1 NCL-TGFBR1 mL 1 mL 163 1 Tuberin 164 mL 1 1) (Type mL Receptor 7 Beta Factor Growth 163 Transforming NCL-L-TTF-1 164 NCL-TRAIL 163 gondii Toxoplasma NCL-TIMP2-487 162 mL 1 NCL-TSH I Topoisomerase NCL-TSH-R2 (TRAIL) Ligand Apoptosis-Inducing mL TNF-Related 1 161 mL PA0025 7 2 Metalloproteinase Matrix of Inhibitor Tissue NCL-TENAS-C mL 0.1 mL, 1 mL 162 1 Factor-1 Transcription Thyroid 162 Receptor Hormone Stimulating Thyroid 162 161 NCL-TARC RTU-TdT-339 Hormone Stimulating Thyroid mL 1 mL 7 mL NCL-TdT-339 0.1 NCL-L-ASYN mL, 1 ready-to-use Bond Thyroglobulin Chemokine 160 Activation-Regulated 160 and Thymus 160 NCL-L-SYNAP-299 mL 0.5 Transferase Deoxynucleotidyl Terminal mL 1 mL 1 Transferase Deoxynucleotidyl Terminal NCL-SP-A PA0299 159 159 C Tenascin 159 NCL-SNAP-25 ready-to-use Bond (TRAP) Phosphatase Acid Tartrate-Resistant NCL-SOD1 mL 7 NCL-SPEC2 Alpha Synuclein, 158 ready-to-use Bond Synaptophysin Synaptophysin RTU-SMA A Protein Surfactant (Cu/Zn) Dismutase Superoxide spectrum) (broad Spectrin SNAP-25 Actin) Muscle Smooth (Alpha SMA 3 nie C-G151mL 1 165 NCL-TG Antigen P30 NCL-TRAP RTU-SYNAP-299 NCL-SYNAP-299 NCL-SV2 NCL-SPPB RE7104 NCL-SPEC1 NCL-SMA PA0943 NCL-TUB-B2 NCL-TROPC NCL-TGFB NCL-TOPOIIA NCL-TRADD NCL-TIMP3 PA0364 NCL-TTF-1 PA0776 NCL-L-TPO NCL-L-THY NCL-CD141 PA0339 NCL-L-TdT-339 NCL-Tau-2 A03117mL 7 161 PA0093 ec irssesrpeettv o viaiiyi orregion. your your in consult availability Please for approval. representative regulatory Microsystems to Leica subject are catalog this in Products 161 160 160 160 159 175 159 158 158 167 166 166 165 164 164 163 163 163 163 162 162 162 162 161 L . mL 0.1 mL, 1 7mL mL 0.1 mL, 1 1mL mL 0.1 mL, 1 mL 25 1mL 1mL 7mL 1mL 1mL mL 0.1 mL, 1 mL 0.1 mL, 1 1mL 1mL 7mL mL 0.1 mL, 1 7mL 1mL 1mL mL 0.1 mL, 1 7mL 1mL mL 0.1 mL, 1 Zap-70 Tumor Wilms' C1P1) (p21, Protein WAF1 ready-to-use Bond Vimentin Unit Vimentin Page Vimentin Villin (VMAT2) 2 Transporter Monoamine Vesicular Code (CD144) VE-Cadherin virus Varicella-zoster Kit Detection ISH Universal mL 1 Ubiquitin 167 Tyrosinase ready-to-use NCL-L-TRAF-1 Bond Tyrosinase Tyrosinase 1 Factor Receptor-Associated Factor Necrosis Tumor Product a-0Bn ed-oueP09 7 mL 7 mL 7 171 mL 1 mL 7 mL 0.1 mL, 1 170 170 mL 170 1 PA0998 170 RTU-VIM-V9 mL 1 170 NCL-VIM NCL-WAF-1 mL 7 PA0562 NCL-L-VIM-V9 170 170 mL NCL-L-VILLIN 1 mL, 2.5 ready-to-use mL Bond 1 Zap-70 PA0106 168 ready-to-use Bond Tumor Wilms' 168 C1P1) (p21, mL Protein NCL-DRP2 0.1 WAF1 mL, 1 mL 0.1 mL, 1 NCL-UBIQm Vimentin mL 1 168 mL 1 Vimentin 169 mL 167 1 Vimentin NCL-L-VMAT1 169 ready-to-use NCL-TH Bond Villin NCL-TYROS 168 NCL-L-VEGFR-3 Villin (VMAT1) 1 Transporter Monoamine Vesicular NCL-TRP-1 Receptor-3 Factor Growth Endothelial Vascular Utrophin Ubiquitin Hydroxylase Tyrosine Protein-1 Tyrosinase-Related Tyrosinase NCL-VILLIN NCL-L-VMAT2 NCL-L-VE-Cad NCL-VZV NCL-ISH-D NCL-UBIQ RTU-TYROS PA0322 NCL-L-TYROS NCL-L-ZAP-70 NCL-L-WT1-562 NCL-L-WAF-1 PA0033 NCL-VIM-V9 NCL-L-VIM-572 W P F etr blotting Western Paraffin Frozen 170 169 169 168 185 168 167 167 167 171 170 170 170 170 170 C I lwcytometry Flow Immunofluorescence L . mL 0.1 mL, 1 mL 0.1 mL, 1 1mL 1mL Tests 100 1mL 7mL 7mL 1mL L . mL 0.1 mL, 1 mL 0.1 mL, 1 1mL 7mL mL 0.1 mL, 1 mL 0.1 mL, 1 O E te applications Other lcrnmicroscopy Electron 219 /
Product Name Index Product Name Index o ealdifraino l rdcspes ii u website: our visit please www.leica-microsystems.com products all on information detailed For 220 / Unit Tests 50 Page Transferase Deoxynucleotidyl 192 Terminal Receptor Progesterone Code ORG-8714 A Melan Receptor Estrogen Cytokeratin CD23 CD8 CD4 CD1a Oncoprotein bcl-2 Product Origin yatpyi R-88165 Tests 50 Tests 50 Tests 50 196 Tests 50 195 195 Tests 50 Tests ORG-8848 194 50 ORG-8772 ORG-8854 Tests 194 50 193 Tests ORG-8889 50 193 ORG-8975 192 ORG-8941 ORG-8919 ORG-8982 Synaptophysin Melanosome Ki67 Desmin CD79a CD10 CD5 CD3 ORG-8721 ORG-8953 ORG-8871 ORG-8735 ORG-8826 ORG-8936 ORG-8756 ORG-8968 ORG-8865 ec irssesrpeettv o viaiiyi orregion. your your in consult availability Please for approval. representative regulatory Microsystems to Leica subject are catalog this in Products 195 195 194 194 193 193 192 192 196 0Tests 50 Tests 50 Tests 50 Tests 50 Tests 50 Tests 50 Tests 50 Tests 50 0Tests 50 W P F etr blotting Western Paraffin Frozen C I lwcytometry Flow Immunofluorescence O E te applications Other lcrnmicroscopy Electron 221 /
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development andcloseindustrycollaboration. leadership, 20yearsofproven“in-house” and ISHyoucantrust,dependonscientifi c paraffi n-embedded (FFPE)tissue.ForIHC bes createdspecifi cally forformalin-fi xed, products aswellantibodiesandpro- diagnostic confi dence withourIVDlabeled systems andadvancedancillaries.Build antibodies, probes,primersets,detection close customercollaboration. turnaround, diagnosticconfi dence, and creates betterpatientcarethroughrapid www.leica-microsystems.com and deliverconsistentlybrilliantresults. gned together, workseamlesslytogether, automation andLISconnectivityaredesi- te IHCandISHsolution.Bondreagents, integrated Bondsystemisthecomple- ve consistentlybrilliantresults-thefully Boost productivity, enhancequality, achie- Bond can relyonclinically-focusedNovocastra When youneedIHCandISHreagents, Novocastra Novocastra tems featuringinnovativeautomationand entire lab.Withcompletehistologysys- productivity workfl ow solutionsforthe product foreachhistologystepandhigh- pathologist, therangeincludesideal prehensive productrange.Frompatientto researchers thehighest-quality, mostcom- Division bringshistopathologylabsand The LeicaMicrosystemsBiosystems Biosystems Division Leica Microsystems “With theuser,foruser” ™ System TM reagents,LeicaMicrosystems ™ Reagents ™
andrepresentativesinmorethan100 countries Active worldwide Science, andContinuousImprovement.Forus,livingup tothesevaluesmeans: brand valuestoliveupthistradition:Pioneering,High-endQuality, Team Spirit,Dedicationto with endusersanddrivingforceofinnovationatLeicaMicrosystems.We havedevelopedfi ve The statementbyErnstLeitzin1907,“withtheuser, fortheuser,” describesthefruitfulcollaboration UA Bnokunllni Tl 1874502 Fax +18474050164 Fax +4686254510 Tel. +18474050123 Fax +6567730628 Tel. +46862545 Fax +41717263444 Bannockburn/lllinois Fax +34934949532 Fax +441908609992 Tel.Tel. +4171726 34 +6567797823 Tel.Tel. +441908246 +34934949530 Fax +351213854668 Tel. +351213889112 Milton Keynes Heerbrugg Kista Fax +8225146548 Barcelona USA: Fax +31704132109 United Kingdom: Tel. +8225146543 Fax +81354212896 Fax +390257403392 Tel. +31704132100 Switzerland: Tel. +81354212800 Lisbon Tel. +3902574861 Fax +85225644163 Sweden: Tel. +85225646699 Spain: Fax +4964412955 Seoul Singapore Tel. +4964412940 00 Rijswijk Fax +4544540111 Tokyo Portugal: Fax +331560523 Milan Hong Kong Tel. +4544540101 People’s Rep.ofChina: Tel. +33811000664 Netherlands: Fax +431486805030 Wetzlar NanterreCedex Korea: Tel. +43148680500 Fax +3227909868 Fax +19057628937 Japan: Herlev Fax +61298781055 Tel. +19057622000 Tel. +3227909850 Italy: Richmond Hill/Ontario Tel. +61288703500 Germany: Groot Bijgaarden France: Vienna Denmark: North Ryde Canada: Belgium: Austria: Australia: Living uptoLife .
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