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Lymphoid Myeloid LYMPHOID Human Hematopoietic Stem Cell MYELOID CD34 CD117 (c-kit) CD338 CD38low/neg CD133 linneg CD59 CD135 (Flt3) GATA2 CD90 (Thy1) CD164 TdT Common Lymphoid Progenitor (CLP) Multi-Potent Progenitor (MPP) Common Myeloid Progenitor (CMP) low neg CD33 CD123low CD174 CD7 CD117 (c-kit) C/EBPα CD34 linneg neg CD34 CD131 Ikaros CD10 CD127 GATA2 CD135 (Flt3) TdT CD24neg CD135 (Flt3) GATA3 CD45RA CD164 PU.1 CD34 CD164 PU.1high Common DC Progenitor CD117 (c-kit) CD173 CD38 HLA-DR TdT CD45RA Aiolos CD11c dim CD90low c-mybhigh CD33 Pro-B Pre-NK/T Megakaryocyte- CD10 CD124 CD5 Conventional DC Precursor Erythroid CD19 CD164 CD34 CD11c Granulocyte-Myeloid CD20 CD252 CD14neg Progenitor (GMP) Progenitor (MEP) CD22 CD268 CD33dim CD33 CD131 CD34neg CD275 CD34 CD45RA CD34 CD164 CD72 HLA-DR CD36 FOG-1 CD74 CD123 PU.1 CD110 GATA1 CD123high GATA2 Pre-T Pro-NK CD7 Pre-B CD1a CD28 CD10neg CD2 CD34 neg CD10 CD124 CD34 CD3 CD45 CD117 (c-kit) CD19 CD164 CD5 CD127 (IL-7Rα) CD20 CD252 CD7high NOTCH1 CD22 CD268 CD34neg CD275 CD72 HLA-DR Immature NK CD74 IgM CD34neg Plasmacytoid Conventional Monocyte Neutrophil Eosinophil Mast Basophil Megakaryocyte Erythrocyte CD79a Pax5 CD94neg DC (pDC) DC (cDC) CD4low CD85a (ILT5) CD171 CD10 CD9 CD9 CD9 CD41 CD51 CD35 CD236 CD117 (c-kit) CD9 CD85d (ILT4) CD172a (SIRPα) CD15 CD11b CD11b CD11a CD42a CD61 CD44 CD236R CD4 CD1c CD122 CD11b CD85h (ILT1) CD172b CD16b CD15 CD15 CD11b CD42b CD110 CD123 CD238 CD11c neg CD1d CD161 CD11c CD85j (ILT2) CD180 CD17 CD24 CD24 CD13 CD42c CD112 CD173 CD239 CD45RA CD2 Ets-1 CD13 CD85k (ILT3) CD184 (CXCR4) CD24 CD32 CD32 CD16 CD42d CD123 CD233 CD240 Immature B CD4 ISP (Immature CD49d CD4low act Id2 CD14 CD86 CD191 (CCR1) CD32 CD35 CD35 CD17 CD49f CD234 CD241 CD5 CD152 CD85g (ILT7) CD11b Single Positive) Id3 CD15 CD87 CD192 (CCR2) CD35 CD64 CD43 CD25 CD235a CD242 CD10 CD153 CD85k (ILT3) CD11c CD1a CD7 CD17 CD89 CD195 (CCR5) CD43 CD244 CD64 CD32 CD235b CD19 CD180 CD123 CD13 CD2 CD34 CD28 CD91 CD198 (CCR8) CD66a CD116 CD116 CD33 CD20 CD184 CD282 (TLR2)neg CD14 neg CD3 CD45RO CD31 CD92 CD210 (IL-10R) CD66b CD123 CD117 (c-kit) CD38 CD21 CD185 CD284 (TLR4)neg CD33 CD4 CD127 (IL-7Rα) CD32 CD93 CD213a1 (IL-13Rα1) CD66d CD125 CD123 CD43 CD22 CD196 low CD289 (TLR9) CD45RO CD5 TCRβ CD33 CD101 CD213a2 (IL-13Rα2) CD89 CD126 CD125 CD88 CD23 CD200 Mature NK CD303 (BDCA-2) CD64 Platelet CD35 CD111 CD226 CD93 FcεR1 CD126 CD123 CD24 CD210 CD2 CD160 CD304 (BDCA-4) CD68 CD9 CD51 CD36 CD112 CD277 CD112 IgE CD181 (CXCR1) CD125 CD32 CD229 CD7 CD161 HLA-DR CD80 CD17 CD60a act CD38 CD115 CD281 (TLR1) CD114 CD244 CD218 (IL-18R) CD37 CD252 CD8 CD162R TLR7 CD85j (ILT2) CD23 CD61 CD40 CD116 CD282 (TLR2) CD116 FcεR1 CD154 CD40 CD253 CD11b CD178 Spi-B CD85k (ILT3) CD31 CD84 CD45RO CD119 CD284 (TLR4) CD157 IgE CD192 CD48 CD267 (TACI) CD15s CD181 (CXCR1) CD86 CD36 CD92 CD45RA CD121b CD300a CD177 CD193 CD52 CD275 CD16 CD182 (CXCR2) CD105 CD41 CD110 CD45RB CD123 CD300c CD181 (CXCR1) CD203c CD72 CD279 (PD-1) CD27 CD183 (CXCR3) CD112 CD42a CD147 CD49b CD131 CD300e (IREM-2) CD281 (TLR1) CD281 (TLR1) CD75 CD307 γδ T CD32 CD198 CD123neg CD42b CD151 CD49e CD142 CD300f (IREM-1) CD282 (TLR2) CD282 (TLR2) CD79a CD317 (BST2) CD4neg CD43 CD210 CD141low CD42c CD173 CD49f CD147 CD302 CD284 (TLR4) CD284 (TLR4) CD79b CD319 (CRACC) γδ TCR CD45RC CD212 CD163 CD42d CD226 act CD63 CD155 CD305 CD286 (TLR6) CD286 (TLR6) CD80 IgD Sox13 CD49d CD218a CD172a (SIRPα) CD49b GARP CD64 CD156a CD312 CD289 (TLR9) CD289 (TLR9) CD84 IgG CD56 CD218b CD195 (CCR5) CD49f act CD65s CD157 CD317 (BST2) MPO CD294 CD86 IgM CD57 CD223 CD197 (CCR7) CD68 CD162 CD322 Trem-1 FcεR1 CD98 CD62L CD226 CD205 CD74 CD163 CD329 IgE CD124 CD66a CD244 CD282 (TLR2) act CD84 CD168 GITRL C/EBPα CD137 (41BB) CD81 CD245 CD283 (TLR3) CD170 PU.1 GATA2 CD85d (ILT4) CD247 CD284 (TLR4) CD85j (ILT2) CD253 CD286 (TLR6) Activated Platelets Early Double CD87 CD277 CD300e CD54 CD107a CD94 CD300a CLEC-9A CD62p CD107b Mature B Positive (EDP) CD96 CD305 HLA-DR CD63 CD109 CD5 CD180 CD1a CD99R CD319 (CRACC) CD19 CD184 (CXCR4) CD2 CD119 CD328 (Siglec-7) CD20 CD185 (CXCR5) CD3 CD122 CD335 (NKp46) Macrophage CD21 CD192 (CCR2)act CD4 CD132 CD336 CD11a CD26 CD52 CD88 CD156a CD206 CD354 (Trem-1) CD22 CD196 (CCR6) CD5 CD158a CD337 CD11c CD31 CD63 CD92 CD163 CD210 CD357 (GITR) CD23act CD200 CD7 CD158b Granzyme A CD14 CD32 CD68 CD119 CD169 CD280 HLA-DR CD24 CD210 CD8α CD158f Granzyme B CD16 CD36 CD71 CD121b CD170 CD282 (TLR2) Trem-2 CD27act CD229 CD38 CD159a (NKG2A) Perforin CD19 CD40 CD74 CD123 CD195 CD284 (TLR4) PU.1 CD32 CD252act CD45RO CD159c (NKG2C) CD23 CD45RO CD87 CD155 CD204 CD312 CD35 CD253act CD127 (IL-7Rα) CD37 CD267 (TACI) TCRβ CD40 CD268 (BAFFR) CD8 CD48act CD269 (BCMA) CD8 CD49b CD272 Granulysin General T Cell Markers CD49c CD275 Granzyme A CD2 CD35 CD60a CD89 CD119 CD158a CD172a (SIRPα) CD196 (CCR6) CD229 CD316 CD49d CD279 (PD-1)act Granzyme B CD3 CD40 CD60b CD94 CD121b CD158b CD181 (CXCR1) CD197 (CCR7) CD244 CD317 (BST2) CD52 CD307 Perforin CD5 CD43 CD60c CD98 CD122 CD158f CD182 (CXCR2) CD198 (CCR8) CD247 CD319 (CRACC) CD72 CD317 (BST2) Runx3 CD6 CD45RA CD62L CD99R CD123 CD159a CD184 (CXCR4) CD199 (CCR9) CD277 αβ TCR CD75 CD319 (CRACC) CD7 CD45RB CD75s CD101 CD127 CD160 CD191 (CCR1) CD210 CD279 CD79a CD360 (IL-21R) CD27 CD45RC CD81 CD103 CD130 CD161 CD192 (CCR2) CDv218a CD300a CD79b HLA-DR Double Positive (DP) CD28 CD52 CD84 CD107aact CD132 CD168 CD193 (CCR3) CD218b CD300c act act CD80 IgD CD1a CD8β CD30 CD57 CD85j (ILT2) CD107b CD150 CD171 CD195 (CCR5) CD226 CD305 CD84 IgG CD2 CD45RO act CD86 IgM CD3 CD127 (IL-7Rα) CD98 Blimp-1 CD4 TCRβ CD124 Bcl-6 CD5 ThPOK act CD137 (41BB) Pax5 CD7 TOX act CD152 (CTLA-4) CD8α CD4 CD4 Plasma NKT CD3 neg CD19 CD133 CD8 neg CD20 CD138 CD16 LEGEND Treg Th17 Th1 Th2 Th9 Th22 Tfh CD27 CD183 (CXCR3) CD56 CD25 CD161 CD26 CD30 CD124 (IL-4R) CD140 (PDGFR) CD84 CD252 CD184 (CXCR4) CD69 –– = Proposed Pathway CD39 CD121a (IL-1R1) CD94 CD124 (IL-4R) IL-17RB CCR10 CD126 (IL-6R) CD52neg CD269 (BCMA) CD161 act = Expressed On Activated CD73 CD196 (CCR6) CD178 (FASL) CD184 (CXCR4) IRF4 CD185 (CXCR5) CD126 Aiolos Vα24Jα18TCR CD101 CD360 (IL-21R) CD195 CD198 (CCR8) CD278 (ICOS) CD38 Blimp-1 low = Low Expresion low Id2 CD127 IL-23R CD212 (IL-12RB2) IL-17RB CD360 (IL-21R) Differentiation of the Human Hematopoietic Stem Cell CD72neg IRF4 PLZF high = High Expresion CD152 (CTLA-4) BATF CD218 (IL-18R) NOTCH1 Bcl-6 Human hematopoietic development from the hematopoietic CD79a XBP-1 CD183 (CXCR3) IRF4 CD254 (TRANCE) NOTCH2 stem cell to the end stage cells illustrating markers found at neg = Negative each stage as determined through the 9th HLDA congress. CD194 (CCR4) RORγt CD278 (ICOS) Tim-1 = Intracellular Antigen CD199 (CCR9) NOTCH3 GATA3 Q312061 Hematopoietic Stem Cells 0912 = Subset CD357 (GITR) Tim-3 act = Pan Marker/Common GARP T-bet Foxp3 Defining Marker Helios ©2012 eBioscience, Inc. An Affymetrix Company. All rights reserved. Differentiation of the Human Hematopoietic Stem Cell Customers in countries where direct sales are not available may contact their eBioscience distributor listed at NORTH AMERICA: CUSTOMER SERVICE 888.999.1371 • [email protected] | TECHNICAL SUPPORT 888.810.6168 • [email protected] www.eBioscience.com/distributors EUROPE: CUSTOMER SERVICE +43 1 796 40 40-304 • [email protected] | TECHNICAL SUPPORT +43 1 796 40 40-120 • [email protected] www.eBioscience.com.
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