Human Enolase 2/Neuron-specific Enolase

Antibody Monoclonal Mouse IgG2A Clone # 972458 Catalog Number: MAB51691

DESCRIPTION Species Reactivity Human

Specificity Detects human Enolase 2/Neuron­specific Enolase in direct ELISAs.

Source Monoclonal Mouse IgG2A Clone # 972458 Purification Protein A or G purified from hybridoma culture supernatant Immunogen E. coli­derived recombinant human Enolase 2/Neuron­specific Enolase Met1­Leu434 Accession # P09104 Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (­SP) is supplied either lyophilized or as a 0.2 μm filtered solution in PBS.

APPLICATIONS Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website. Recommended Sample Concentration Western Blot 0.5 µg/mL See Below Immunocytochemistry 1­25 µg/mL See Below

DATA

Western Blot Immunocytochemistry Detection of Human, Mouse, and Rat Enolase 2/Neuron­specific Enolase in SH­ Enolase 2/Neuron­specific Enolase by SY5Y Human Cell Line. Enolase 2/Neuron­ Western Blot. Western blot shows lysates of specific Enolase was detected in immersion Jurkat human acute T cell leukemia cell line, fixed SH­SY5Y human neuroblastoma cell SH­SY5Y human neuroblastoma cell line, line using Mouse Anti­Human Enolase human brain tissue (cerebellum), mouse 2/Neuron­specific Enolase Monoclonal brain tissue (cerebellum), and rat brain tissue Antibody (Catalog # MAB51691) at 1 µg/mL (cerebellum). PVDF membrane was probed for 3 hours at room temperature. Cells were with 0.5 µg/mL of Mouse Anti­Human stained using the NorthernLights™ 557­ Enolase 2/Neuron­specific Enolase conjugated Anti­Mouse IgG Secondary Monoclonal Antibody (Catalog # MAB51691) Antibody (red; Catalog # NL007) and followed by HRP­conjugated Anti­Mouse IgG counterstained with DAPI (blue). Specific Secondary Antibody (Catalog # HAF018). A staining was localized to cytoplasm and specific band was detected for Enolase plasma membrane. View our protocol for 2/Neuron­specific Enolase at approximately Fluorescent ICC Staining of Cells on 47 kDa (as indicated). This experiment was Coverslips. conducted under reducing conditions and using Immunoblot Buffer Group 1.

PREPARATION AND STORAGE Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS. Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (­SP) is shipped with polar packs. Upon receipt, store it immediately at ­20 to ­70 °C Stability & Storage Use a manual defrost freezer and avoid repeated freeze­thaw cycles. l 12 months from date of receipt, ­20 to ­70 °C as supplied. l 1 month, 2 to 8 °C under sterile conditions after reconstitution. l 6 months, ­20 to ­70 °C under sterile conditions after reconstitution.

BACKGROUND Enolase 2 (2­phospho­D­glycerate hydrolyase; also Neuron­specific Enolase, NSE, neural enolase and γ­enolase) is a 46 kDa member of the Enolase family of enzymes. It is expressed in developing neurons and glia, is known to catalyze the generation of phosphoenolpyruvate, and is suggested to possess neurotrophic activity for neurons, likely through an extracellular mechanism. Human Enolase 2 is 434 amino acids (aa) in length. The enzymatic site spans most of the length of the molecule. Enolase 2 exists as both a noncovalently­linked homodimer, or heterodimer with α­enolase. Full­length human Enolase 2 shares 99% aa identity with both mouse and canine Enolase 2. It shares 83% aa identity with human enolases 1 and 3.

Rev. 2/7/2018 Page 1 of 1