Using Comparative Genomics to Identify Virulence Traits and Vaccine Candidates in Mannheimia Haemolytica
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Characterization of the Genetic Diversity and Antimicrobial Resistance in Mannheimia Haemolytica from Feedlot Cattle
CHARACTERIZATION OF THE GENETIC DIVERSITY AND ANTIMICROBIAL RESISTANCE IN MANNHEIMIA HAEMOLYTICA FROM FEEDLOT CATTLE CASSIDY L. KLIMA Bachelor of Science, University of Lethbridge, 2007 A Thesis Submitted to the School of Graduate Studies of the University of Lethbridge in Partial Fulfillment of the Requirements for the Degree MASTER OF SCIENCE (Molecular Biology/ Microbiology) Department of Biological Sciences University of Lethbridge LETHBRIDGE, ALBERTA, CANADA © Cassidy L. Klima, 2009 DEDICATION This work is in dedication to all of the friends and family that have been an amazing support over the past years. I am sure I will look back at this experience in all fondness and they will look back and say “Thank goodness that it is over with!” I love you all! iii ABSTRACT: Characterization of the genetic diversity and antimicrobial resistance in Mannheimia haemolytica from feedlot cattle Mannheimia haemolytica is an opportunistic pathogen in cattle and the main bacterial agent in bovine respiratory disease. Despite its economic importance, few studies have characterized the genetic diversity of M. haemolytica, particularly from feedlots. Three genotyping techniques (BOX-PCR, (GTG)5-PCR and PFGE) were compared to discriminate M. haemolytica and strains from the family Pasteurellaceae. PFGE was the most discriminating and repeatable, although BOX-PCR was most accurate in clustering isolates together according to species. Mannheimia haemolytica was isolated from nasal swab samples collected from cattle upon entry and exit from two feedlots in southern Alberta. These were characterized by PFGE and antimicrobial susceptibility using a disk-diffusion assay. Select gene determinants were screened for using PCR. PFGE analysis revealed the isolates to be highly diverse. -
Comparative and Functional Genomic Studies of Histophilus Somni
Comparative and Functional Genomic Studies of Histophilus somni (Haemophilus somnus) Shivakumara Siddaramappa Dissertation submitted to the faculty of the Virginia Polytechnic Institute and State University in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Biomedical and Veterinary Sciences Dr. Thomas J. Inzana, Chair Dr. Stephen M. Boyle Dr. Xiang-Jin Meng Dr. Biswarup Mukhopadhyay Dr. Brett M. Tyler Monday, April 9 2007 Blacksburg, Virginia, United States of America Keywords: Haemophilus, Histophilus, Genome, Bacteriophage, Plasmid, LuxS, Biofilm, Restriction-Modification Copyright © 2007, Shivakumara Siddaramappa Comparative and Functional Genomic Studies of Histophilus somni (Haemophilus somnus) Shivakumara S. Siddaramappa ABSTRACT Histophilus somni is a commensal of the mucosal surfaces of respiratory and reproductive tracts of cattle and sheep. However, as an opportunistic pathogen, H. somni can cause diseases such as pneumonia, myocarditis, abortion, arthritis, and meningo-encephalitis. Previously, several virulence factors/mechanisms had been identified in H. somni of which the phase- variable lipooligosaccharide, induction of host cell apoptosis, intraphagocytic survival, and immunoglobulin Fc binding proteins were well characterized. To further understand the biological properties of H. somni, the genomes of pneumonia strain 2336 and preputial strain 129Pt have been sequenced. Using the genome sequence data and comparative analyses with other members of the Pasteurellaceae, putative genes that encode proteases, restriction- modification enzymes, hemagglutinins, glycosyltransferases, kinases, helicases, and adhesins have been identified in H. somni. Most of the H. somni strain-specific genes were found to be associated with prophage-like sequences, plasmids, and/or transposons. Therefore, it is likely that these mobile genetic elements played a significant role in creating genomic diversity and phenotypic variability among strains of H. -
Identification of Pasteurella Species and Morphologically Similar Organisms
UK Standards for Microbiology Investigations Identification of Pasteurella species and Morphologically Similar Organisms Issued by the Standards Unit, Microbiology Services, PHE Bacteriology – Identification | ID 13 | Issue no: 3 | Issue date: 04.02.15 | Page: 1 of 28 © Crown copyright 2015 Identification of Pasteurella species and Morphologically Similar Organisms Acknowledgments UK Standards for Microbiology Investigations (SMIs) are developed under the auspices of Public Health England (PHE) working in partnership with the National Health Service (NHS), Public Health Wales and with the professional organisations whose logos are displayed below and listed on the website https://www.gov.uk/uk- standards-for-microbiology-investigations-smi-quality-and-consistency-in-clinical- laboratories. SMIs are developed, reviewed and revised by various working groups which are overseen by a steering committee (see https://www.gov.uk/government/groups/standards-for-microbiology-investigations- steering-committee). The contributions of many individuals in clinical, specialist and reference laboratories who have provided information and comments during the development of this document are acknowledged. We are grateful to the Medical Editors for editing the medical content. For further information please contact us at: Standards Unit Microbiology Services Public Health England 61 Colindale Avenue London NW9 5EQ E-mail: [email protected] Website: https://www.gov.uk/uk-standards-for-microbiology-investigations-smi-quality- and-consistency-in-clinical-laboratories UK Standards for Microbiology Investigations are produced in association with: Logos correct at time of publishing. Bacteriology – Identification | ID 13 | Issue no: 3 | Issue date: 04.02.15 | Page: 2 of 28 UK Standards for Microbiology Investigations | Issued by the Standards Unit, Public Health England Identification of Pasteurella species and Morphologically Similar Organisms Contents ACKNOWLEDGMENTS ......................................................................................................... -
Prevalence and Antibiotic Susceptibility of Mannheimia
Veterinary World, EISSN: 2231-0916 RESEARCH ARTICLE Available at www.veterinaryworld.org/Vol.13/September-2020/28.pdf Open Access Prevalence and antibiotic susceptibility of Mannheimia haemolytica and Pasteurella multocida isolated from ovine respiratory infection: A study from Karnataka, Southern India Swati Sahay1,2 , Krithiga Natesan1, Awadhesh Prajapati1, Triveni Kalleshmurthy1 , Bibek Ranjan Shome1, Habibur Rahman3 and Rajeswari Shome1 1. Indian Council of Agricultural Research-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, Karnataka, India; 2. Department of Microbiology, Centre for Research in Pure and Applied Sciences, Jain University, Bengaluru, Karnataka, India; 3. International Livestock Research Institute, CG Centre, NASC Complex, DPS Marg, Pusa, New Delhi, India. Corresponding author: Rajeswari Shome, e-mail: [email protected] Co-authors: SS: [email protected], KN: [email protected], AP: [email protected], TK: [email protected], BRS: [email protected], HR: [email protected] Received: 25-04-2020, Accepted: 29-07-2020, Published online: 23-09-2020 doi: www.doi.org/10.14202/vetworld.2020.1947-1954 How to cite this article: Sahay S, Natesan K, Prajapati A, Kalleshmurthy T, Shome BR, Rahman H, Shome R (2020) Prevalence and antibiotic susceptibility of Mannheimia haemolytica and Pasteurella multocida isolated from ovine respiratory infection: A study from Karnataka, Southern India, Veterinary World, 13(9): 1947-1954. Abstract Background and Aim: Respiratory infection due to Mannheimia haemolytica and Pasteurella multocida are responsible for huge economic losses in livestock sector globally and it is poorly understood in ovine population. The study aimed to investigate and characterize M. haemolytica and P. multocida from infected and healthy sheep to rule out the involvement of these bacteria in the disease. -
How Mannheimia Haemolytica Defeats Host Defence Through a Kiss of Death Mechanism Laurent Zecchinon, Thomas Fett, Daniel Desmecht
How Mannheimia haemolytica defeats host defence through a kiss of death mechanism Laurent Zecchinon, Thomas Fett, Daniel Desmecht To cite this version: Laurent Zecchinon, Thomas Fett, Daniel Desmecht. How Mannheimia haemolytica defeats host de- fence through a kiss of death mechanism. Veterinary Research, BioMed Central, 2005, 36 (2), pp.133- 156. 10.1051/vetres:2004065. hal-00902968 HAL Id: hal-00902968 https://hal.archives-ouvertes.fr/hal-00902968 Submitted on 1 Jan 2005 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Vet. Res. 36 (2005) 133–156 133 © INRA, EDP Sciences, 2005 DOI: 10.1051/vetres:2004065 Review article How Mannheimia haemolytica defeats host defence through a kiss of death mechanism Laurent ZECCHINON, Thomas FETT, Daniel DESMECHT* Department of Pathology, Faculty of Veterinary Medicine, University of Liège, FMV Sart-Tilman B43, 4000 Liège, Belgium (Received 22 June 2004; accepted 6 October 2004) Abstract – Mannheimia haemolytica induced pneumonias are only observed in goats, sheep and cattle. The bacterium produces several virulence factors,whose principal ones are lipopolysaccharide and leukotoxin. The latter is cytotoxic only for ruminant leukocytes, a phenomenon that is correlated with its ability to bind and interact with the ruminant β2-integrin Lymphocyte Function-associated Antigen 1. -
Review on the Potential Effects of Mannheimia Haemolytica and Its Immunogens on the Female Reproductive Physiology and Performance of Small Ruminants
Journal of Animal Health and Production Review Article A Review on the Potential Effects of Mannheimia haemolytica and its Immunogens on the Female Reproductive Physiology and Performance of Small Ruminants 1,2 2 1,3 FAEZ FIRDAUS ABDULLAH JESSE *, MOHAMED ABDIRAHMAN BOOREI , ERIC LIM TEIK CHUNG , 2 4 2 2 FITRI WAN-NOR , MOHD AZMI MOHD LILA , MOHD JEFRI NORSIDIN , KAMARULRIZAL MAT ISA , 1 1 5 6 NUR AZHAR AMIRA , ARSALAN MAQBOOL , MOHAMMED NAJI ODHAH , YUSUF ABBA , ASINAMAI 7 6 6 2,6 ATHLIAMAI BITRUS , IDRIS UMAR HAMBALI , INNOCENT DAMUDU PETER , BURA THLAMA PAUL 1Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; 2Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; 3Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; 4Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; 5Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Pengakalan Chepa 16100, Kota Bharu, Kelantan, Malaysia; 6Faculty of Veterinary Medicine, University of Maiduguri, PMB 1069 Maiduguri, Borno Nigeria; 7Faculty of Veterinary Science, University of Jos, P.M.B 2084 Jos, Plateau Nigeria. Abstract | Mannheimia haemolytica causes pneumonic pasteurellosis (mannheimiosis) in ruminants which is the most economically significant infectious disease. Mannheimia belongs to the family Pasteurellaceae, are nonmotile, non- spore-forming, facultatively anaerobic, oxidase-positive and fermentative gram-negative rods or coccobacilli which are frequent respiratory and digestive tract commensals in both domestic and wild animals. They can produce infection in animals with compromised immune states. -
Occurrence of Haemolytic Mannheimia Spp. in Apparently Healthy Sheep In
Acta Veterinaria Scandinavica BioMed Central Research Open Access Occurrence of haemolytic Mannheimia spp. in apparently healthy sheep in Norway Louise L Poulsen1, Turið M Reinert1, Rikke L Sand1, Magne Bisgaard1, Henrik Christensen1, John E Olsen1, Snorre Stuen2 and Anders M Bojesen*1 Address: 1Department of Veterinary Pathobiology, The Royal Veterinary and Agricultural University, 4 Stigbøljen, DK-1870 Frederiksberg C, Copenhagen, Denmark and 2Norwegian School of Veterinary Science, Department of Production Animal Clinical Sciences, Sandnes, Norway Email: Louise L Poulsen - [email protected]; Turið M Reinert - [email protected]; Rikke L Sand - [email protected]; Magne Bisgaard - [email protected]; Henrik Christensen - [email protected]; John E Olsen - [email protected]; Snorre Stuen - [email protected]; Anders M Bojesen* - [email protected] * Corresponding author Published: 31 October 2006 Received: 27 June 2006 Accepted: 31 October 2006 Acta Veterinaria Scandinavica 2006, 48:19 doi:10.1186/1751-0147-48-19 This article is available from: http://www.actavetscand.com/content/48/1/19 © 2006 Poulsen et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: The occurrence of Mannheimia species in healthy sheep has only been investigated to a very limited extend since the genus and its five named species were established. The aim of the present study was to evaluate the occurrence of haemolytic Mannheimia species in apparently healthy sheep originating from four sheep flocks in South-Western Norway. -
CHARACTERIZATION of MANNHEIMIA HAEMOL JT/C4-SPECIFIC BACTERIOPHAGES YU-HUNG HSU Bachelor of Science, University of British Colum
CHARACTERIZATION OF MANNHEIMIA HAEMOL JT/C4-SPECIFIC BACTERIOPHAGES YU-HUNG HSU Bachelor of Science, University of British Columbia, 2007 A Thesis Submitted to the School of Graduate Studies of the University of Lethbridge in Partial Fulfillment of the Requirements for the Degree MASTER OF SCIENCE (Molecular Biology/ Microbiology) Department of Biological Sciences University of Lethbridge LETHBRIDGE, ALBERTA, CANADA © Yu-Hung Hsu, 2011 Library and Archives Bibliotheque et Canada Archives Canada Published Heritage Direction du Branch Patrimoine de I'edition 395 Wellington Street 395, rue Wellington Ottawa ON K1A0N4 Ottawa ON K1A 0N4 Canada Canada Your file Votre reference ISBN: 978-0-494-88386-0 Our file Notre reference ISBN: 978-0-494-88386-0 NOTICE: AVIS: The author has granted a non L'auteur a accorde une licence non exclusive exclusive license allowing Library and permettant a la Bibliotheque et Archives Archives Canada to reproduce, Canada de reproduire, publier, archiver, publish, archive, preserve, conserve, sauvegarder, conserver, transmettre au public communicate to the public by par telecommunication ou par I'lnternet, preter, telecommunication or on the Internet, distribuer et vendre des theses partout dans le loan, distrbute and sell theses monde, a des fins commerciales ou autres, sur worldwide, for commercial or non support microforme, papier, electronique et/ou commercial purposes, in microform, autres formats. paper, electronic and/or any other formats. The author retains copyright L'auteur conserve la propriete du droit d'auteur ownership and moral rights in this et des droits moraux qui protege cette these. Ni thesis. Neither the thesis nor la these ni des extraits substantiels de celle-ci substantial extracts from it may be ne doivent etre imprimes ou autrement printed or otherwise reproduced reproduits sans son autorisation. -
Histophilus Somni-Induced Thrombotic Meningoencephalitis in Cattle from Northern Paraná, Brazil1
Pesq. Vet. Bras. 35(4):329-336, abril 2015 DOI: 10.1590/S0100-736X2015000400003 Histophilus somni-induced thrombotic meningoencephalitis in cattle from northern Paraná, Brazil1 Selwyn A. Headley2*, Ana Paula F.R.L. Bracarense2, Victor H.S. Oliveira3, Gustavo R. Queiroz4, Werner Okano5 3, Karina K.M.C. Flaiban6, Júlio A.N. Lisbôa4 3 , Alice F. Alfieri ABSTRACT.- and Amauri A. Alfieri Histophilus somni-induced throm- botic meningoencephalitis Headley S.A., Bracarense in cattle A.P.F.R.L., from northern Oliveira Paraná, V.H.S., Queiroz Brazil. G.R., Pesquisa Okano Veterinária W., Alfieri BrasileiraA.F., Flaiban 35(4):329-336. K.K.M.C., Lisbôa Laboratório J.A.N. & Alfieride Patologia A.A. 2015. Animal, Departamento de Medicina Vete- rinária Preventiva, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, PR-445 Km [email protected] Thrombotic meningoencephalitis (TME) is a fatal neurological disease of cattle, predo- 380,minantly Cx. Postal from 10.011,North America, Londrina, that PR 860571-970,is caused by Brazil.Histophilus E-mail: somni with sporadic descrip- tions from other countries. This manuscript describes the occurrence of spontaneous TME in cattle from northern Paraná, Brazil. Most cattle had acute neurological manifestations suggestive of bacterial infections of the brain. Histopathology revealed meningoencephalitis characteristicwith vasculitis of and brain thrombosis dysfunction. of small Hematological vessels that and contained cerebrospinal discrete fluid neutrophilic analyses were and/or not nerve ganglion of all animals. All tissues from the central nervous system used during this lymphocytic infiltrates admixed with fibrin at the brainstem, cerebral cortex, and trigeminal- orescence assay. -
Changes in the Rates of Field Isolation and Antimicrobial Susceptibility Of
PEER REVIEWED Changes in the rates of field isolation and antimicrobial susceptibility of bacterial pathogens collected from fall placed feedlot steers between arrival at the feedlot and 90 to 120 days on feed Nathan E.N. Erickson,1 MVetSc, DVM, BSc; Musangu Ngeleka,2 PhD, MSc, DVM; Brian V. Lubbers,3 PhD, Diplomate ACVCP, DVM; Anatoliy Trokhymchuk,4 MSc, DVM 1Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, 52 Campus Drive, Saskatoon SK S7N 5B4 Canada 2Prairie Diagnostic Services and Department of Veterinary Microbiology, Western College of Veterinary Medicine, 52 Campus Drive, Saskatoon SK S7N 5B4 Canada 3Kansas State Veterinary Diagnostic Laboratory and Department of Diagnostic Medicine/Pathobiology, 1800 Denison Ave., Manhattan, KS 66506 4Prairie Diagnostic Services, 52 Campus Drive, Saskatoon SK S7N 5B4 Canada Corresponding author: Dr. Nathan E.N.Erickson,[email protected] Abstract bovins (Mannheimia haemolytica, Pasteurella multocida et Histophilus somni) ont ete observees a l'aide d'ecouvillons The change in bacterial recovery and phenotypic an nasaux profonds chez 295 veaux en sante dans un pare timicrobial susceptibility of 3 important bovine respiratory d' engraissement en automne dans l' ouest Canadien entre disease bacteria (Mannheimia haemolytica, Pasteurella mul leur arrivee et de 90 a 120 jours plus tard. Au moment de tocida, and Histophilus somni) between arrival and 90 to 120 l'echantillonnage a l'arrivee, le pourcentage de veaux avec days was observed in 295 healthy fall-placed feedlot calves des cultures positives etait de 28% pour Mannheimia hae in western Canada using deep nasal swabs. At the arrival molytica, de 28% pour Pasteurella multocida et de 8% pour sampling, 28%, 28%, and 9% of calves were culture posi Histophilus somni; les pourcentages apres l'arrivee (note du tive for Mannheimia haemolytica, Pasteurella multocida, and traducteur: environ 90 jours apres l'arrivee) etaient de 23%, Histophilus somni, respectively; these rates changed to 23%, 14% et 16%, respectivement. -
Development of Intranasal Bacterial Therapeutics to Mitigate the Bovine Respiratory Pathogen Mannheimia Haemolytica
University of Calgary PRISM: University of Calgary's Digital Repository Graduate Studies The Vault: Electronic Theses and Dissertations 2019-11 Development of Intranasal Bacterial Therapeutics to Mitigate the Bovine Respiratory Pathogen Mannheimia haemolytica Amat, Samat Amat, S. (2019). Development of Intranasal Bacterial Therapeutics to Mitigate the Bovine Respiratory Pathogen Mannheimia haemolytica (Unpublished doctoral thesis). University of Calgary, Calgary, AB. http://hdl.handle.net/1880/111258 doctoral thesis University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission. Downloaded from PRISM: https://prism.ucalgary.ca UNIVERSITY OF CALGARY Development of Intranasal Bacterial Therapeutics to Mitigate the Bovine Respiratory Pathogen Mannheimia haemolytica by Samat Amat A THESIS SUBMITTED TO THE FACULTY OF GRADUATE STUDIES IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY GRADUATE PROGRAM IN VETERINARY MEDICAL SCIENCES CALGARY, ALBERTA NOVEMBER, 2019 © Samat Amat 2019 Abstract The emergence of multidrug-resistant pathogens associated with bovine respiratory disease (BRD) presents a significant challenge to the beef industry, as antibiotic administration is commonly used to prevent and control BRD in commercial feedlot cattle in North America. Thus, developing antibiotic alternatives such as bacterial therapeutics (BTs) to mitigate BRD is needed. Recent studies suggest that the nasopharyngeal (NP) microbiota, particularly lactic acid-producing bacteria (LAB), are important to bovine respiratory health and may be a source of BTs for the inhibition of BRD pathogens. -
Dynamics and Stabilization of the Rumen Microbiome in Yearling
www.nature.com/scientificreports OPEN Dynamics and stabilization of the rumen microbiome in yearling Tibetan sheep Lei Wang1,2,4, Ke Zhang3,4, Chenguang Zhang3, Yuzhe Feng1, Xiaowei Zhang1, Xiaolong Wang 3 & Guofang Wu1,2* The productivity of ruminants depends largely on rumen microbiota. However, there are few studies on the age-related succession of rumen microbial communities in grazing lambs. Here, we conducted 16 s rRNA gene sequencing for bacterial identifcation on rumen fuid samples from 27 Tibetan lambs at nine developmental stages (days (D) 0, 2, 7, 14, 28, 42, 56, 70, and 360, n = 3). We observed that Bacteroidetes and Proteobacteria populations were signifcantly changed during the growing lambs’ frst year of life. Bacteroidetes abundance increased from 18.9% on D0 to 53.9% on D360. On the other hand, Proteobacteria abundance decreased signifcantly from 40.8% on D0 to 5.9% on D360. Prevotella_1 established an absolute advantage in the rumen after 7 days of age. The co-occurrence network showed that the diferent microbial of the rumen presented a complex synergistic and cumbersome relationship. A phylogenetic tree was constructed, indicating that during the colonization process, may occur a phenomenon in which bacteria with close kinship are preferentially colonized. Overall, this study provides new insights into the colonization of bacterial communities in lambs that will beneft the development of management strategies to promote colonization of target communities to improve functional development. Rumen microbes are essential for ruminant health. Ruminants rely upon the action of microbial fermentation in the rumen to digest plant fbers and convert some nutrients that cannot be directly utilized into animal proteins for host utilization1.