Journal of Life Sciences and Technologies Vol. 3, No. 1, June 2015 Mechanical Properties and Biocompatibility of Attacus atlas and Bombyx mori Silk Fibers Released from Cocoons by Alkali Treatment Tjokorda Gde Tirta Nindhia and I. Wayan Surata Department of Mechanical Engineering, Udayana University, Jimbaran, Bali, Indonesia, 80361 Email:
[email protected] Zdeněk Knejzlík and Tomáš Ruml Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague, Technická 5, 166 28, Prague, Czech Republic Tjokorda Sari Nindhia Faculty of veterinary Madicine, Udayana University, Jl. P.B. Sudirman, Denpasar, Bali, Indonesia, 80114 Abstract—Natural silks, produced by spiders and insects, historically used in textile industry [6]. Fibers present in represent perspective source of biomaterials for the cocoons are mainly composed from fibroin complex regenerative medicine and biotechnology because of their [7] produced from paired labial glands [8]. About 10 – 12 excellent biocompatibility and physico-chemical properties. μm fibroin fibers in B. mori cocoon are tethered by It was previously shown that silks produced by several amorphous protein; sericin, which can be released from members of Saturniidae family have excellent properties in comparison to silk from B. mori, the most studied silkworm. cocoon by washing in mild alkali conditions or hot water, Efficient degumming of silk fibers is a critical step for a process, designated as degumming [9]. Fine fibers, subsequent processing of fibers and/or fibroin. In this study, obtained by degumming, can be used as source of fibroin we describe cheap, environmentally friendly and efficient which may be next solubilized in denaturing agents such NaOH-based degumming of A. atlas fibers originated from as highly concentrated solution of lithium salts, calcium natural cocoon.