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Chicken Anti-Anemia Virus (AV) ELISA Kit, Cat # EK-920-110-AV ELISA Kit Features

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Chicken Anti-Anemia Virus (AV) ELISA Kit, Cat # EK-920-110-AV ELISA Kit Features Chicken Anti-Anemia Virus (AV) ELISA Kit, Cat # EK-920-110-AV _ ELISA Kit Features • Purified Anemia Virus (AV) viral antigens-coated, stabilized, 96-well strip plate (8x12 strips) • Negative and Positive controls • 100ul samples (diluted 1:100 or more) • 105 min , 3 incubation step at room temp • Detects antibody titer in the egg yolks of vaccinate or immunized chickens (no need for blood samples) or in serum/plasma. • Shelf life >6 months or more. This kit is for measuring anti-AV Antibody in chicken serum or plasma or egg yolks . For in vitro research use only . Assay Procedure: Allow all reagents to reach room temperature. Arrange and label required number of strips. Step 1. Pipet 100 ul each of negative and positive controls , and diluted samples into wells. Mix gently and incubate at room temperature for 60 min. Step 2. Aspirate and wash the plate four times. Add 100ul of Antibody-HRP Conjugate to all wells, mix gently and incubate at room temperature for 30 min. Step 3 . Aspirate and wash the plate five times. Add 100 ul of TMB Substrate solution to all wells, mix gently, and incubate at room temperature for 15 min. Step 4 . Pipet 100 ul of stop solution into each well and mix gently (blue color turns yellow). Measure OD at A450 nm . Determine the presence of chicken anti-Influenza A IgG by comparing with the supplied –ve control and positive calibrator. General Information Chicken anaemia virus, or CAV, is a member of the circoviridae family. It is a non- enveloped icosahedral single stranded DNA virus. Their genomes are the smallest possessed by animal viruses. The capsid of CAV consists of a single type of protein VP1, which encapsidates a negative-strand genome of about 2,300 bases (The following popper user interface control may not be accessible. Tab to the next button to revert the control to an accessible version.Destroy user interface control19). VP1, which has a molecular mass of 50 kDa, has an extremely basic N-terminal region of about 50-aa. Infection causes anaemia, bone marrow atrophy, and severe immunosuppression in poultry as the virus affects the production of red blood cells (RBC), white blood cells (WBC) and platelets. Clinical signs of CAV infection are predominantly found in young chicks due to vertical transmission from the breeder hens whose maternal antibodies have not yet formed following exposure. Clinical disease is rare today because of the widespread practice of vaccinating breeders, but the subclinical form of the disease - which normally affects birds more than two weeks of age following horizontal transmission of the virus via the oro-faecal route - is ubiquitous. Additionally, the virus is very resistant in the environment, making elimination very difficult. It is not a zoonosis. The vaccine has the ATCvet code QI01AD04. Vaccination of antibody-negative breeder flocks prior to the start of egg production is recommended. Nobilis CAV P4 is a live attenuated vaccine against Chicken. Anaemia Virus. CIRCOMUNE® W is a live chicken Infectious Anemia Virus vaccine, Del Ros strain, for administration in the drinking water. The disease/virus has many names including chicken anaemia, blue wing disease, anaemia dermatitis syndrome, chicken/avian infectious anaemia, haemorrhagic aplastic anaemia syndrome, infectious chicken anaemia, chicken infectious anaemia virus and chicken anaemia agent. Vertical spread of the disease can be controlled by the vaccination of breeding hens with both live attenuated and wild vaccines. These vaccines reduce the vertical transmission rate. Appropriate hygiene and biosecurity measures should be employed to control the disease. In general, acute viral infections can be diagnosed by detection of the virus (viral antigens) itself or the specific antibody response viral antigens. The most common assays for routine use of virus detection are virus isolation (VI), immunofluorescence assay (IFA), immunoperoxidase assay (IPA), polymerase chain reaction (PCR), and for antibody detection the haemagglutination inhibition (HI) test, agar gel precipitation test (AGPT/AGID), and enzyme linked immunosorbent assay (ELISA). The virus neutralization test (VNT) is rarely used for routine diagnosis because it is relatively expensive and laborious. An assessment of immune status, as well as serological identification of viral infection requires a measurement of antibody to the virus in serum. Enzyme-linked immunosorbent assay (ELISA) systems have proven efficacious in the quantification of antibody levels to IBV, and facilitate the monitoring of immune status in large flocks. GSI’s Chicken Anemia Virus (AV) ELISA Kit is a simple, rapid, and sensitive method to detect chicken AV infection or to measure efficacy of AV vaccines (V-IBV, P-IBV). Related Items EK-920-100-AIV Chicken Anti-Avian Influenza A virus (AIV) IgG ELISA kit, 2x 96 tests, Quantitative EK-920-105-AIM Chicken Anti-Avian Influenza A virus (AIV) IgM ELISA kit, 2x 96 tests, Quantitative EK-920-110-AV Chicken Anti-Anemia Virus (AV) IgG ELISA kit, 2x96 tests EK-920-120-IBV Chicken Anti-Infectious Bronchitis Virus (IBV) IgG ELISA kit, 2x96 tests EK-920-130-MDV Chicken Anti-Marek’s Disease Virus (MDV) IgG ELISA kit, 2x96 tests EK-920-140-NDV Chicken Anti-Newcastle Disease Virus (NDV) IgGs ELISA kit, 2x96 tests EK-920-300-H51 Chicken Anti-Avian Influenza virus (H5N1) IgG ELISA kit (1x96 wells) EK-920-300-H52 Chicken Anti-Avian Influenza virus (H5N1) IgG ELISA kit, 5x96 tests, Quantitative, 5x96 tests Rev. 120712A GENEMED SYNTHESIS, INC. 6203 Wood Lake Center Dr., Bldg. 2, San Antonio, TX 78244, USA Toll free (800) 344-5337; Phone : (210) 745-5988; Fax (210) 745-5992 www.genemedsyn.com .
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