DOCSLIB.ORG

IIIHIII IIII US005474896A United States Patent (19) 11) Patent Number: 5,474,896 Dujon Et Al

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
IIIHIII IIII US005474896A United States Patent (19) 11) Patent Number: 5,474,896 Dujon Et Al IIIHIII IIII US005474896A United States Patent (19) 11) Patent Number: 5,474,896 Dujon et al. (45. Date of Patent: Dec. 12, 1995 54 NUCLEOTIDE SEQUENCE ENCODING THE 185-187 (1980). ENZYME-SCE AND THE USES THEREOF Michel, F. et al., Comparison of fungal mitochondrial introns reveals extensive homologies in RNA secondary 75) Inventors: Bernard Dujon, Gif sur Yvette; Andre structure, Biochimie, vol. 64: 867-881 (1982). Choulika, Paris, both of France; Michel, F. and Dujon, B., Conservation of RNA secondary Laurence Colleaux, Edinburgh, structures in two intron families including mitochondrial-, Scotland; Cecile Fairhead, Malakoff, chloroplast- and nuclear-encoded members, EMBO Jour France; Arnaud Perrin, Paris, France; nal, vol. 2(1): 33-38 (1983). Anne Plessis, Paris, France; Agnes Jacquier, A. and Dujon, B., The Intron of the Mitochondrial Thierry, Paris, France 21s rRNA Gene: Distribution in Different Yeast Species and Sequence Comparison Between Kluyveromyces thermotol 73 Assignees: Institut Pasteur; Université Paris-VI, erans and Saccharomyces cerevisiae, Mol. Gen. Genet., both of France 192: 487-499 (1983). Dujon, B. and Jacquier, A., Mitochondria 1983, Walter de (21) Appl. No.: 971,160 Gruyter & Co., pp. 389-403. Jacquier, A. and Dujon, B., An Intron-Encoded Protein is 22 Filed: Nov. 5, 1992 Active in a Gene Conversion Process That Spreads an Intron into a Mitochondrial Gene, CELL, vol. 41: 383-394 (1985). Related U.S. Application Data Dujon, B. et al., in Achievements and Perspectives of 63 Continuation-in-part of Ser. No. 879,689, May 5, 1992, Mitochondrial Research, vol. II: Biogenesis, Elsevier Sci abandoned. ence Publishers, pp. 215-225 (1985). Colleaux, L. et al., Universal Code Equivalent of a Yeast (51) Int. Cl. ............................. C12Q 1/68; C12N 15/70 52 U.S. Cl. ............... 435/6; 435/320.1; 935/78 Mitochondrial Intron Reading Frame is Expressed into E. 58) Field of Search ............................ 435/6, 255,320.1; coli as a Specific Double Strand Endonuclease, CELL, vol. 935/77, 78 44:521-533 (1986). Michel, F and Dujon, B., Genetic Exchanges between 56) References Cited Bacteriophase T4 and Filamentous Fungi?, CELL, vol. 46: PUBLICATIONS 323 (1986). Tartot et al., Gene 67:169-182 (1988) “New cloning vectors (List continued on next page.) and techniques . '. Primary Examiner-Margaret Parr Colleaux et al, Human Mol. Genet. 2(3):265-271 (1993) Assistant Examiner-Eggerton Campbell "Rapid Mapping of YAC inserts...'. Attorney, Agent, or Firm-Finnegan, Henderson, Farabow, Dujon, Bernard "Group I introns as mobile genetic ele Garrett & Dunner ments: facts and mechanistic speculations-a review,' GENE, vol. 82: 91-114 (1989). 57 ABSTRACT Dujon, Bernard “Mobile Introns', EMBO Workshop on An isolated DNA encoding the enzyme I-SceI is provided. Molecular Mechanisms of Transposition and Its Control, Roscoff, France, Jun. 24-28, 1990. The DNA sequence can be incorporated in cloning and Dujon, Bernard "Des Introns Autonomes et Mobiles', expression vectors, transformed cell lines and transgenic Annales de L'Institut Pasteur/Actualites, i:181-194 (1990). animals. The vectors are useful in gene mapping and site Dujon, B., Sequence of the Intron and Flanking Exons of the directed insertion of genes. Mitochondrial 21s rBNA Gene of Yeast Strains Having Different Alleles at the co and rib-1 Loci, CELL, vol. 20: 2 Claims, 22 Drawing Sheets AAAAATAAAATCAT AG AAA AAT ATT AAA AAA AA CAA GA ATC AAT CTC 66 CCAA TC M. K. N. I K K N G W M N G P N S AAA TTA TTA AAA GAA TAT AAA CA CAA TA AT GAA TA AAT ATT GAA CAA TT GAA GCA K K E Y K S Q E L E G F E A GGT AT GGTTTA AT TTA. GGA GAT GCTA A CGT AG CGT GAT GAA GGT AAA AC TAT G I. 6 L G D A Y R S R B E S K T Y GT AG CAA TTT CAC TCC AAA AA AAG GCA TAC ATG GAT CAT GA TGT TTA TTA, TA GAT C M F. E. W. K. N. K. A Y M D H W C Y D CAA TGG GTATTACA CCT CCT CAT AAA AAA GAA AGA is AA CA TTA. GGT AAT AGA Q W W L S P P H K K E R W N G. N. L. W. AT ACC TGG GGA GCT CAA ACT TT AAA CAT CAA GOT TAAT AAA TTA GCT. AAC A TT K A F M K L A AT GTA AAT AAT AAA AAA CT ACC AA AAT TA GT GAA AA TA TTA ACA CC ATG Y N N K K L P N N L W E N Y T P M AGT CG GCA TA, TGG TTT ATG GA GA GGA GGT AAA TGG GAAT AAAAA AAT C CT S A Y W F M D D G G K D Y N K. N S AA AAA AGT A GTA TTA AAT ACA CAA AG T ACT TT GAA GAA GTA GAA AA CT N K S W N T Q S F F E E W C Y W AAA GGT TA AGA AATAAA TTT CAA TTA AA TG TAT ST AAA ATT AATAAA AATAAA CCA K G R N K F Q C Y V K K N K P At A TATAT GAT TCT AG, AGT TAT. CTG AT TTT TAT AAT TA At AAA CCT TAT TTA Y I D S M S Y F Y N K P Y L. ATT CCT CAA AGATS TATAAA CTG CCAA AC AT TCA CC GAA ACTA AAA TAA I P G M M Y K L N S S E F L K 5,474,896 Page 2 OTHER PUBLICATIONS Saccharomyces and Neurospora introns, Mol. Gen. Genet., Colleaux, L. et al., Recognition and cleavage site of the vol. 223: 288-296 (1990). intron-encoded omega transposase, Proc. Natl. Acad. Sci. Dujon, B. et al., in Extrachromosomal Elements in Lower USA, vol. 85: 6022-6026 (1988). Eukaryotes, (Plenum Publishing Corporation 1986), pp. Dujon, B. et al., Mobile introns: definition of terms and 5-27. recommended nomenclature, GENE, vol. 82: 115-118 (1989). Thierry, A. et al., Cleavage of yeast and bacteriophage T7 Monteilhet, C. et al., Purification and characterization of the genomes at a single site using the rare cutter endonuclease in vitro activity of I-Scel, a novel and highly specific I-Sce, Nucleic Acids Research, vol. 19(1): 189-190 (1991). endonuclease encoded by a group 1 intron, Nucleic Acids Plessis, A. et al., Site-specific recombination by I-Scel; a Research, vol. 18(6), 1407-1413 (1990. mitochondrial group 1 intron-encoded endonuclease Colleaux, L. et al., The apocytochrome b gene of Chlamy expressed in the yeast nucleus, Genetics, vol. 130(3): domonas smithi contains a mobile intron related to both 451-460 (1992). U.S. Patent Dec. 12, 1995 Sheet 3 of 22 5,474,896 severely affected A AA i partially affected i unoffected 5' i:GT TA CGC III:ATAA 3' 3' CAAT GCGAT AT 5' U.S. Patent Dec. 12, 1995 Sheet 6 of 22 5,474,896 -2 -1 5 10 M. H M K N I K K N G V M N L G P N S 20 30 K K E Y K S Q L I E L N E Q F E A LO 50 G G L I L G D A Y I R S R D E G K T Y 60 70 C M Q F E W K N K A Y M D H W C L L Y C 80 90 Q W Y L S P P H K K E R Y N H L G N L Y 100 10 I T W G A Q T F K H Q A F N K L A N L. F 120 130 I V N N K K I I P N N L V E N Y L T P M 140 150 G L A Y W P M D D G G K W D Y N K N S I 160 170 N K S I W L N T Q S F T F E E W E Y L. W 180 190 K G L R N K F Q. L N C Y V K N. K. N. K. P 200 20 I I Y D S M S Y L I F Y N I K P Y L. 220 230 P Q M M Y K L P N T S S E T F L K * POSitions that Can be Changed Without affecting enzyme activity (demonstrated) positions -l and -2 are not natural, The two amino acids are added due to ClOning Strategies pOSlt). OnS Ug 10; Can be deleted position 35: G is tolerated position 40: M or V are tolerated position 41: S or N are tolerated position 43: A is tolerated position 46: W or N are tolerated position 91A is tolerated positions 123 and 156; L are tolerated pOSition 225; A and S are tolerated Changes that affect enzyme activity (demon Strated) position 19; L to S position 38: t O S position 39: position 4Q: position 42: p0Sition 44: O H position 45: position 46: position pOSlton rr position | position F.G. 5 pOSition pOSiti On : U.S. Patent Dec. 12, 1995 Sheet 7 of 22 5,474,896 Group I intron Encoded Endonucleases and Redted Endonucleoses CLEAVAGE ENDONUCLEASE RECOGNITION SEQUENCE SITE V INTRON SITE I-Sce I-Sce IV (Saccharomyces mitochondrio) I Sce I (Saccharomyces mitochondria) (ChlamydomonasI-Ceu chloroplast)I I-Ppo I ( Phys OU C leus (SaccharomycesI-Sce mitochondrio) II (ChlamydomonasI-Cre chloroplast) Endo.
Load more

Recommended publications
  • DE-Kupl: Exhaustive Capture of Biological Variation in RNA-Seq Data Through K-Mer Decomposition
    Audoux et al. Genome Biology (2017) 18:243 DOI 10.1186/s13059-017-1372-2 METHOD Open Access DE-kupl: exhaustive capture of biological variation in RNA-seq data through k-mer decomposition Jérôme Audoux1, Nicolas Philippe2,3, Rayan Chikhi4, Mikaël Salson4, Mélina Gallopin5, Marc Gabriel5,6, Jérémy Le Coz5,EmilieDrouineau5, Thérèse Commes1,2 and Daniel Gautheret5,6* Abstract We introduce a k-mer-based computational protocol, DE-kupl, for capturing local RNA variation in a set of RNA-seq libraries, independently of a reference genome or transcriptome. DE-kupl extracts all k-mers with differential abundance directly from the raw data files. This enables the retrieval of virtually all variation present in an RNA-seq data set. This variation is subsequently assigned to biological events or entities such as differential long non-coding RNAs, splice and polyadenylation variants, introns, repeats, editing or mutation events, and exogenous RNA. Applying DE-kupl to human RNA-seq data sets identified multiple types of novel events, reproducibly across independent RNA-seq experiments. Background diversity is genomic variation. Polymorphism and struc- Successive generations of RNA-sequencing technologies tural variations within transcribed regions produce RNAs have bolstered the notion that organisms produce a highly with single-nucleotide variations (SNVs), tandem dupli- diverse and adaptable set of RNA molecules. Modern cations or deletions, transposon integrations, unstable transcript catalogs, such as GENCODE [1], now include microsatellites, or fusion events. These events are major hundreds of thousands of transcripts, reflecting pervasive sources of transcript variation that can strongly impact transcription and widespread alternative RNA processing. RNA processing, transport, and coding potential.
    [Show full text]
  • 2010-Rolland Et Al.Pdf
    Dynamic evolution of megasatellites in yeasts. Thomas Rolland, Bernard Dujon, Guy-Franck Richard To cite this version: Thomas Rolland, Bernard Dujon, Guy-Franck Richard. Dynamic evolution of megasatellites in yeasts.. Nucleic Acids Research, Oxford University Press, 2010, 38 (14), pp.4731-9. 10.1093/nar/gkq207. pasteur-01370686 HAL Id: pasteur-01370686 https://hal-pasteur.archives-ouvertes.fr/pasteur-01370686 Submitted on 23 Sep 2016 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Distributed under a Creative Commons Attribution - NonCommercial| 4.0 International License Published online 31 March 2010 Nucleic Acids Research, 2010, Vol. 38, No. 14 4731–4739 doi:10.1093/nar/gkq207 Dynamic evolution of megasatellites in yeasts Thomas Rolland1,2,3, Bernard Dujon1,2,3 and Guy-Franck Richard1,2,3,* 1Institut Pasteur, Unite´ de Ge´ ne´ tique Mole´ culaire des Levures, Department ‘Genomes and Genetics’, 25 rue du Dr Roux, 2CNRS, URA2171 and 3Universite´ Pierre et Marie Curie, UFR 927, F-75015 Paris, France Received January 13, 2010; Revised March 10, 2010; Accepted March 12, 2010 ABSTRACT homology with any other tandem repeat or gene sequenced so far. Among the 84 minisatellites previously Megasatellites are a new family of long tandem reported in Saccharomyces cerevisiae (3), four harbor a repeats, recently discovered in the yeast Candida tandemly repeated motif of 135 bp or larger, and may glabrata.
    [Show full text]
  • Transcriptional Mechanisms of Resistance to Anti-PD-1 Therapy
    Author Manuscript Published OnlineFirst on February 13, 2017; DOI: 10.1158/1078-0432.CCR-17-0270 Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Transcriptional mechanisms of resistance to anti-PD-1 therapy Maria L. Ascierto1, Alvin Makohon-Moore2, 11, Evan J. Lipson1, Janis M. Taube3,4, Tracee L. McMiller5, Alan E. Berger6, Jinshui Fan6, Genevieve J. Kaunitz3, Tricia R. Cottrell4, Zachary A. Kohutek7, Alexander Favorov8,10, Vladimir Makarov7,11, Nadeem Riaz7,11, Timothy A. Chan7,11, Leslie Cope8, Ralph H. Hruban4,9, Drew M. Pardoll1, Barry S. Taylor11,12,13, David B. Solit13, Christine A Iacobuzio-Donahue2,11, and Suzanne L. Topalian5 From the 1Departments of Oncology, 3Dermatology, 4Pathology, 5Surgery, 6The Lowe Family Genomics Core, 8Oncology Bioinformatics Core, and the 9 Sol Goldman Pancreatic Cancer Research Center, Johns Hopkins University School of Medicine and Sidney Kimmel Comprehensive Cancer Center, Baltimore, MD 21287; the 10Laboratory of System Biology and Computational Genetics, Vavilov Institute of General Genetics, Russian Academy of Sciences, 119991, Moscow, Russia; and 2Pathology, 7Radiation Oncology, 11Human Oncology and Pathogenesis Program, 12Department of Epidemiology and Biostatistics, and the 13Center for Molecular Oncology, Memorial Sloan Kettering Cancer Center, New York NY 10065. MLA, AM-M, EJL, and JMT contributed equally to this work Running title: Transcriptional mechanisms of resistance to anti-PD-1 Key Words: melanoma, cancer genetics, immunotherapy, anti-PD-1 Financial Support: This study was supported by the Melanoma Research Alliance (to SLT and CI-D), the Bloomberg~Kimmel Institute for Cancer Immunotherapy (to JMT, DMP, and SLT), the Barney Family Foundation (to SLT), Moving for Melanoma of Delaware (to SLT), the 1 Downloaded from clincancerres.aacrjournals.org on October 2, 2021.
    [Show full text]
  • Rolland and Dujon, 2011
    C. R. Biologies 334 (2011) 620–628 Contents lists available at ScienceDirect Comptes Rendus Biologies www.sciencedirect.com Evolution/E´ volution Yeasty clocks: Dating genomic changes in yeasts Horloges tremblantes : datation des changements ge´nomiques chez les levures Thomas Rolland, Bernard Dujon * Unite´ de ge´ne´tique mole´culaire des levures (CNRS URA2171 and University P.-M.-Curie UFR927), Institut Pasteur, 25, rue du Docteur-Roux, 75724 Paris cedex 15, France ARTICLE INFO ABSTRACT Article history: Calibration of clocks to date evolutionary changes is of primary importance for Received 12 November 2010 comparative genomics. In the absence of fossil records, the dating of changes during Accepted after revision 17 March 2011 yeast genome evolution can only rely on the properties of the genomes themselves, given Available online 1 July 2011 the uncertainty of extrapolations using clocks from other organisms. In this work, we use the experimentally determined mutational rate of Saccharomyces cerevisiae to calculate Keywords: the numbers of successive generations corresponding to observed sequence polymor- Evolution phism between strains or species of other yeasts. We then examine synteny conservation Mutational rate Polymorphism across the entire subphylum of Saccharomycotina yeasts, and compare this second clock Divergence times based on chromosomal rearrangements with the first one based on sequence divergence. A Synteny conservation non-linear relationship is observed, that interestingly also applies to insects although, for equivalent sequence divergence, their rate of chromosomal rearrangements is higher than that of yeasts. ß 2011 Acade´mie des sciences. Published by Elsevier Masson SAS. All rights reserved. RE´ SUME´ Mots cle´s: L’e´talonnage d’horloges mole´culaires pour dater les changements e´volutifs a une grande E´ volution importance pour la ge´nomique comparative.
    [Show full text]
  • MUC4/MUC16/Muc20high Signature As a Marker of Poor Prognostic for Pancreatic, Colon and Stomach Cancers
    Jonckheere and Van Seuningen J Transl Med (2018) 16:259 https://doi.org/10.1186/s12967-018-1632-2 Journal of Translational Medicine RESEARCH Open Access Integrative analysis of the cancer genome atlas and cancer cell lines encyclopedia large‑scale genomic databases: MUC4/MUC16/ MUC20 signature is associated with poor survival in human carcinomas Nicolas Jonckheere* and Isabelle Van Seuningen* Abstract Background: MUC4 is a membrane-bound mucin that promotes carcinogenetic progression and is often proposed as a promising biomarker for various carcinomas. In this manuscript, we analyzed large scale genomic datasets in order to evaluate MUC4 expression, identify genes that are correlated with MUC4 and propose new signatures as a prognostic marker of epithelial cancers. Methods: Using cBioportal or SurvExpress tools, we studied MUC4 expression in large-scale genomic public datasets of human cancer (the cancer genome atlas, TCGA) and cancer cell line encyclopedia (CCLE). Results: We identifed 187 co-expressed genes for which the expression is correlated with MUC4 expression. Gene ontology analysis showed they are notably involved in cell adhesion, cell–cell junctions, glycosylation and cell signal- ing. In addition, we showed that MUC4 expression is correlated with MUC16 and MUC20, two other membrane-bound mucins. We showed that MUC4 expression is associated with a poorer overall survival in TCGA cancers with diferent localizations including pancreatic cancer, bladder cancer, colon cancer, lung adenocarcinoma, lung squamous adeno- carcinoma, skin cancer and stomach cancer. We showed that the combination of MUC4, MUC16 and MUC20 signature is associated with statistically signifcant reduced overall survival and increased hazard ratio in pancreatic, colon and stomach cancer.
    [Show full text]
  • Applications of Xâ
    Downloaded from genesdev.cshlp.org on June 30, 2009 - Published by Cold Spring Harbor Laboratory Press ‘IN n e-fr £ Development Targeted mutagenesis by homologous recombination in D. melanogaster Yikang S. Rong, Simon W. Titen, Heng B. Xie, et al. G e n e s D e v . 2002 16: 1568-1581 Access the most recent version at doi:10.1101/gad.986602 References This article cites 43 articles, 24 of which can be accessed free at: http://genesdev.cshlp.org/content/16/12/1568.fuN.html#ref-Nst-1 Article cited in: http://genesdev.cshlp.org/content/16/12/1568.full.html#related-urls Email alerting Receive free email alerts when new articles cite this article - sign up in the box at the service top right corner of the article or click here To subscribe to Genes & Development go to: http://genesdev.cshlp.org/subscriptions Cold Spring Harbor Laboratory Press Downloaded from genesdev.cshlp.org on June 30, 2009 - Published by Cold Spring Harbor Laboratory Press m utagenesis by recom bination in D . m elanogaster Yikang S. Rong,1,2,4 Simon W. Titen,1,2 Heng B. Xie,1,2 Mary M. Golic,1,2 Michael Bastiani,1 Pradip Bandyopadhyay,1 Baldomero M. Olivera,1 Michael Brodsky,3,5 Gerald M. Rubin,3 and Kent G. Golic1,2,6 departm ent of Biology, University of Utah, Salt Lake City, Utah 84112, USA; 2Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA; 3Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA Wc used a recently developed method to produce mutant alleles of five endogenous D r o s o p h ila genes, including the homolog of the p 5 3 tumor suppressor.
    [Show full text]
  • The Genomics Journey
    The genomics journey DYGEVO Professor Bernard Dujon discusses his research on yeasts and the usefulness of this family as model organisms in cutting-edge genetic manipulation experiments With such hypotheses, detailed knowledge has indicate that structural genome alterations been gained about the effect of population (segmental duplications, inversions, segmental size. But yeasts are unicellular fungi adapted insertion and deletion, gene loss) occur much to phases of very rapid clonal expansion under more rapidly than anticipated by classical favourable conditions, intermingled with phases methods. They tend to create more genetic of massive cell death. A glass of beer contains variations on a limited timescale than the more yeast individuals than the world’s human classically considered changes based on population. This abundance allows us to work sequence evolution. In addition, structural on rare genetic events without having to wait genome alterations create an abrupt, stepwise very long periods of time. pattern of evolution instead of a seemingly continuous spectrum of variation, more in line For what reasons do unicellular eukaryotic with original Hugo de Vries principles proposed genomes often prefer clonal expansions back in 1909 than with neo-Darwinian ideas on over regular reproduction cycles? gradual, permanent optimisation of the fittest. Many organisms seem to favour clonal How can the flexibility of yeast genomes expansion over sexual reproduction as be exploited to increase cell fitness when soon as conditions are appropriate. During simple nucleotide changes have a low Could you provide some context to your the sunny season, some insects engage in probability of accomplishing such a task? current project examining yeast genome parthenogenesis, and many plants propagate diversity – DYGEVO? What are your clonally.
    [Show full text]
  • Xenopus Transgenic Nomenclature Guidelines
    This document was last updated: October 23rd, 2014. Executive Summary: Xenopus Transgenic Nomenclature Guidelines These guidelines cover the naming of transgenic constructs developed and used in Xenopus research, and the naming of mutant and transgenic Xenopus lines . ● Only those transgenic (Tg) constructs that are supplied to other researchers, or are maintained by a lab or at a stock center, require standardized names following these guidelines. ● Xenbase will record and curate transgenes using these rules. ● Transgenic construct names are italicized following this basic formulae: Tg(enhancer or promoter:ORF/gene) ● Multiple cassette on the same plasmid are separated by a comma within the parenthesis. Tg(enhancer or promoter:ORF/gene, enhancer or promoter:ORF/gene) cassette 1 cassette 2 ● Transgenic Xenopus lines are named based on their unique combination of Tg constructs (in standard text) and indicate Xenopus species and the lab of origin: Xl.Tg(enhancer or promoter:ORF/gene, enhancer or promoter:ORF/gene)#ILAR lab code species cassette 1 cassette 2 lab code ● Xenopus research labs that generate Tg animals are identified using lab codes registered with the ILAR which maintains a searchable database for model organism laboratories. Section 1. Provides an overview for Naming Tg Constructs Section 2. Outlines specific rules for Naming Tg Constructs Section 3. Provides an overview for Naming Tg Xenopus lines and Xenopus lines with mutant alleles. Section 4. Provides guidelines for naming enhancer and gene trap constructs, and lines using these constructs. Section 5. Outlines rules for naming transposon-induced mutations and inserts. Section 6. Discusses disambiguation of wild type Xenopus strains versus transgenic and mutant Xenopus lines.
    [Show full text]
  • Sciellin Antibody / SCEL (F54353)
    Sciellin Antibody / SCEL (F54353) Catalog No. Formulation Size F54353-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml F54353-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml Bulk quote request Availability 1-3 business days Species Reactivity Human Format Purified Clonality Polyclonal (rabbit origin) Isotype Rabbit Ig Purity Antigen affinity purified UniProt O95171 Localization Cytoplasmic, plasma membrane Applications Immunohistochemistry (FFPE) : 1:25 Western blot : 1:500-1:2000 Flow cytometry : 1:25 (1x10e6 cells) Limitations This Sciellin antibody is available for research use only. IHC testing of FFPE human skin tissue with Sciellin antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining. IHC testing of FFPE human esophagus tissue with Sciellin antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining. Western blot testing of human A431 cell lysate with Sciellin antibody. Predicted molecular weight ~77 kDa. Flow cytometry testing of fixed and permeabilized human A431 cells with Sciellin antibody; Blue=isotype control, Green= Sciellin antibody. Description The protein encoded by this gene is a precursor to the cornified envelope of terminally differentiated keratinocytes. This protein localizes to the periphery of cells and may function in the assembly or regulation of proteins in the cornified envelope. Transcript variants encoding different isoforms exist. A transcript variant utilizing an alternative polyA signal has been described in the literature, but its full-length nature has not been determined. Application Notes The stated application concentrations are suggested starting points.
    [Show full text]
  • Repair of a Specific Double-Strand Break Generated Within a Mammalian Chromosome by Yeast Endonuclease L-Scel
    .,. 1994 Oxford University Press Nucleic Acids Research, 1994, Vol. 22, No. 25 5649-5657 Repair of a specific double-strand break generated within a mammalian chromosome by yeast endonuclease l-Scel Tamas Lukacsovich, Di Yang and Alan S.Waldman* Department of Biological Sciences, University of South Carolina, Columbia, SC 29208, USA Received August 29, 1994; Revised and Accepted November 4, 1994 ABSTRACT We established a mouse Ltk- cell line that contains yeast (8), underscoring the importance of mechanisms for efficient within its genome a herpes simplex virus thymidine repair of DSBs. kinase gene (tk) that had been disrupted by the Eukaryotic cells potentially have several mechanisms for insertion of the recognition sequence for yeast repairing a DSB. One possible repair pathway is through endonuclease l-Scel. The artificially introduced 18 bp recombination between the broken sequence and an intact l-Scel recognition sequence was likely a unique homologous sequence. It is well documented that DSBs can sequence in the genome of the mouse cell line. To stimulate extrachromosomal homologous recombination in yeast assess whether an induced double-strand break (DSB) (reviewed in 9) and mammalian cells (reviewed in 10). Several in the genomic tk gene would be repaired preferentially reports on recombination in manunalian cells suggest that a DSB by gene targeting or non-homologous recombination, repair mechanism similar to that originally described for we electroporated the mouse cell line with recombination in yeast (11) also operates in extrachromosomal endonuclease l-Scel alone, one of two different gene recombination of DNA sequences transfected into mammalian targeting constructs alone, or with l-Scel in conjunction cells (10), as well as in gene targeting (12-14).
    [Show full text]
  • A New Class of Large Tandem Repeats Discovered in the Pathogenic Yeast Candida Glabrata Guy-Franck Richard, Agnès Thierry, Bernard Dujon
    Megasatellites: a new class of large tandem repeats discovered in the pathogenic yeast Candida glabrata Guy-Franck Richard, Agnès Thierry, Bernard Dujon To cite this version: Guy-Franck Richard, Agnès Thierry, Bernard Dujon. Megasatellites: a new class of large tandem repeats discovered in the pathogenic yeast Candida glabrata. Cellular and Molecular Life Sciences, Springer Verlag, 2010, 67 (5), pp.671-6. 10.1007/s00018-009-0216-y. pasteur-01370699 HAL Id: pasteur-01370699 https://hal-pasteur.archives-ouvertes.fr/pasteur-01370699 Submitted on 23 Sep 2016 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Cellular and Molecular Life Sciences For Review Only MEGASATELLITES: A NEW CLASS OF LARGE TANDEM REPEATS DISCOVERED IN THE PATHOGENIC YEAST CANDIDA GLABRATA Journal: Cellular and Molecular Life Sciences Manuscript ID: Draft Manuscript Type: Visions and Reflections Yeast, Minisatellite, Cell wall, Megasatellite, Genome, Replication, Key Words: Recombination Page 1 of 13 Cellular and Molecular Life Sciences Cellular and Molecular Life Sciences (2009)
    [Show full text]
  • (12) United States Patent (10) Patent No.: US 6,610,545 B2 Dujon Et Al
    USOO66 10545B2 (12) United States Patent (10) Patent No.: US 6,610,545 B2 Dujon et al. (45) Date of Patent: *Aug. 26, 2003 (54) NUCLEOTIDE SEQUENCE ENCODING THE Michel F, and Dujon B., Conservation of RNA Secondary ENZYME-SCE AND THE USES THEREOF Structure in Two Intron Families Including Mitochondrial-, Chloroplast- and Nuclear-Encloded Members, Embo Jour (75) Inventors: Bernard Dujon, Gif sur Yvette (FR); nal, vol. 2(1), pp. 33–38 (1983). Andre Choulika, Paris (FR); Laurence Dujon, B., and Jacquier, A., Mitochondria, 1983, Walter de Colleaux, Edinburgh (GB); Cecile Gruyter & Co., pp. 389-403. Fairhead, Malakoff (FR); Arnaud Jacquier, A. and Dujon, B., An Intron-Encoded Protein is Perrin, Paris (FR); Anne Plessis, Paris Active in a Gene Conversion Process that Spreads an Intron (FR); Agnes Thierry, Paris (FR) into a Mitochondrial Gene, Cell, vol. 41, pp. 383-394 (1985). (73) Assignees: Institut Pasteur, Paris (FR); University Dujon et al., “In Achievements and Perspective of Mito Paris VI, Paris (FR) chondrial Research', Biogenesis, vol. II, Elsevier Science Publishers, pp. 215-225 (1985). (*) Notice: Subject to any disclaimer, the term of this Colleaux, L. et al., Universal Code Equivalent of a Yeast patent is extended or adjusted under 35 Mitochondrial Intron Reading Frame is Expressed into E. U.S.C. 154(b) by 0 days. Coli as a Specific Double Strand Endonuclease, Cell, vol. 44:521-533 (1986). This patent is Subject to a terminal dis Michel, F. and Dujon, B., “Genetic Exchanges Between claimer. Bacteriophage T4 and Filamentous Fungi'?”, Cell, vol. 46, p. 323 (1986). (21) Appl.
    [Show full text]