CD Antigens 1993: an Updated Nomenclature for Clusters of Differentiation on Human Cells* IUIS/WHO Subcommittee on CD Nomenclature'

CD antigens 1993: an updated nomenclature for clusters of differentiation on human cells* IUIS/WHO Subcommittee on CD Nomenclature'

The 5th International Workshop on Human Leukocyte Differentiation Antigens (Boston, USA, 3-7 November 1993) recommended the adoption of 48 new CD clusters and subclusters and the redefinition of 14 previously established clusters. The additions and changes made to the existing CD nomenclature are summarized in a Table.

Introduction in the latter instance, the letter R is followed by a letter corresponding to relevant exons. The nomenclature for clusters of differentiation (CD), which is dedicated to the designation of cell surface structures of human cells, was established by Results the International Workshop on Human Leukocyte Differentiation Antigens and continues to be updated The results of the 5th Intemational Workshop on by these Workshops. The CD nomenclature was ini- Human Leukocyte Differentiation Antigens were tially proposed to cluster monoclonal antibodies presented on 3-7 November 1993 at a meeting in (mAbs) of different sources that recognize the same Boston, MA, USA. The participants represented the human leukocyte differentiation antigen. The nomen- efforts of more than 500 laboratories worldwide, clature is based on analysis of the results of blinded which joined together over a two-year period to ana- studies of the molecular mass of antigens recognized lyse 1450 antibodies and characterize over 150 mole- by mAbs submitted for evaluation to the Workshop cules. Blind panels for all monoclonal antibodies and of the reactivity of the mAbs with target cells. (mAbs), every CD antigen, every known candidate The nomenclature was aimed to be simple, unambi- for CD status, and all mAbs of undefined specificity guous and adaptable. were analysed by flow cytometry. Other dedicated The CD nomenclature is now widely used to laboratories undertook serological, molecular, bio- designate the molecular species recognized by a chemical and histochemical characterization of the mAb. New mAbs are characterized and therefore mAbs and the structures they defined. The results new CD antigens are defined after each International obtained by all groups showed almost perfect Workshop. In addition, the CDw ("Workshop") des- concordance. ignation is attributed to either (1) a single mAb rec- Detailed results of these studies will be pub- ognizing the cell surface product of a transfected lished separately.a In addition, a Leukocyte Differen- gene, or (2) a new molecule identified by one or two tiation Antigen Database (LDAD) has been devel- mAbs generated in the same laboratory. The CD # R oped to (1) provide identifying information on all symbols are given to indicate that the tissue distribu- molecules and mAbs studied in the Workshop, and tion of CD # may be restricted. In some cases the (2) display/analyse the quantitative expression of restriction has been attributed to alternative splicing; each molecule on more than 80 cell types.b Based on these findings, the Workshop recommends the adoption of 48 new CD clusters and subclusters and the redefinition of 14 previously established clusters. * This report has been approved by the IUIS/WHO Nomencla- The additions and changes made to the existing CD ture Committee. A resume in French appears on page 808. nomenclature are summarized in Table 1. Requests for reprints should be addressed to the Chairman of the IUIS Nomenclature Committee, Professor Michel Kazatchki- ne, Unite d'Immunologie, H6pital Broussais, 96 rue Didot, 75014

Paris, France. a 1 Schlossmann SF et al., eds. White cell differentiation anti- The Subcommittee's members are S.F. Schlossmann (Chair- gens. Oxford, Oxford University Press, 1994 (in press). man), L. Boumsell, W. Gilks, J.M. Harlan, T. Kishimoto, C. Mori- b moto, J. Ritz, S. Shaw, R.L. Silverstein, T.A. Springer, T.F. Ted- The LDAD program runs on IBM PCs and via emulation on der and R.F. Todd. Macintosh. It may be downloaded freely via anonymous ftp from balrog.nci.nih.gov (156.40.182.2) or purchased on disk (inquiries Reprint No. 5532 by fax 301-480-2052).

Bulletin of the World Health Organization, 1994, 72 (5): 807-808 807 IUIS/WHO Subcommittee on CD Nomenclature

Table 1: CD antigens 1993 Table 1: continued CD Desig- Common Workshop Mol. mass CD99 E2, MIC2 T Cell 32 nation name section reduced CD99R CD99 mAb restricted T Cell 32 CD100 BB18, A8 T Cell 150 CD1 5s sLex, Sialyl Lewis x Adhesion CDw1O1 BB27, BA27 T Cell 140 CD16 FcR IIIA/FcR IIIB Myeloid 50-65 CD102 ICAM-2 Adhesion 60 CD1 6b FcR IIIB Myeloid 48 CD1 03 HML-1 Adhesion 150,25 CD32 Previously CDw32, FcRII Myeloid 40 CD104 f4 integrin chain Adhesion 220 CD42a GPIX Platelets 23 CD42b GPIB, a Platelets 135,23 CD105 Endoglin Endothelial 95 CD42c GP1 B-I Platelets 22 CD106 VCAM-1, INCAM-110 Endothelial 100,110 CD42d GPV Platelets 85 CD107a LAMP-1 Platelet 110 CD44 Pgp-1 Adhesion 80-90 CD107b LAMP-2 Platelet 120 CD44R Restricted epitope on CD44 Adhesion CDw1 08 Adhesion 80 CD49a VLA-1, al integrin chain Adhesion 210 CDw1 09 8A3, 7D1 Endothelial 170,150 CD49b VLA-2, a2 integrin chain Adhesion 160 CD1 15 CSF-1R; M-CSFR Myeloid 150 CD49c VLA-3, a3 integrin chain Adhesion 125 CDwl 16 HGM-CSFR, GM-CSFR Cytokine 75-85 CD49d VLA-4, a4 integrin chain Adhesion 150,80,70 CD1 17 SCFR, cKIT Cytokine 145 CD49e VLA-5, a5 integrin chain Adhesion 135,25 CDwl 19 IFNyR Cytokine 90 CD49f VLA-6, a6 integrin chain Adhesion 120,25 CD120a TNFR;55kD Cytokine 55 CD50 ICAM-3 Adhesion 124 CD120b TNFR;75kD Cytokine 75 CD51/ Complex dependent epitope Adhesion CDwl 21 a IL-1 R;Typel Cytokine 80 CD61 CDwl21b IL-1 R;Type2 Cytokine 68 CD52 Campath-1 Blind 21-28 CD122 IL-2R;75kD, IL-2RP Cytokine 75 CDw1 24 IL-4R Cytokine 140 CD62E ELAM-1 Adhesion 115 E-selectin, CD126 IL-6R Cytokine 80 CD62L L-selectin, LAM-1, TQ-1 Adhesion 75-80 CDw1 27 IL-7R Cytokine 75 CD62P P-selectin, GMP-140, Adhesion 150 CDw1 28 IL-8R Cytokine 57-67 PADGEM CDw1 30 IL-6R-GP130SIG Cytokine 130 CD66a BGP Myeloid 180-200 CD66b CD67, p100, CGM6 Myeloid 95-100 CD66c NCA Myeloid 90-95 CD66d CGM1 Myeloid 30 CD66e CEA, carcinoembryonic Myeloid 180-200 Resume antigen Les CD en 1993: nomenclature r6visee CD67 Now CD66b CD70 CD27-ligand Activation 55,75,95, des antigenes de classe de diff6renciation 110,170 pr6sents sur les cellules humaines CDw76 Previously CD76 B Cell NA La nomenclature applicable aux antigenes des CD79a mb-1, Iga B Cell 33,40 CD79b B29, lgt B Cell 33,40 classes de diff6renciation (CD), qui consiste a CD80 B7, BB1 B Cell 60 d6signer les structures presentes a la surface des CD81 TAPA-1 B Cell 22 cellules humaines, a 6t6 6tablie et est r6guliere- CD82 R2, IA4, 4F9 B Cell 50-53 ment mise a jour a l'occasion d'ateliers internatio- CD83 HB15 B Cell 43 naux sur les antigenes de differenciation des leu- CDw84 B Cell 73 cocytes humains. La nomenclature repose sur CD85 VMP-55, GH11/75 B Cell 120,83 deux 6l6ments: les r6sultats d'6tudes men6es en CD86 FUN-1, BU63 B Cell 80 aveugle sur la masse mol6culaire d'antigenes CD87 UPA-R Myeloid 50-65 reconnus par des anticorps monoclonaux (ACM) CD88 C5aR Myeloid 42 soumis pour 6valuation a l'atelier, et la reactivite CD89 FcaR Myeloid 55-75 des ACM avec leurs cellules cibles. CDw9O Thy-1 Myeloid 25-35 Le 5e atelier international sur les antigenes CD91 a2M-R Myeloid 600 de diff6renciation des leucocytes humains (3-7 CDw92 Myeloid 70 novembre 1993, Boston, Etats-Unis d'Am6rique) a CD93 Myeloid 120 recommand6 I'adoption de 48 classes et sous- CD94 KP43 NK Cell 43 classes nouvelles de differenciation ainsi que la CD95 APO-1, FAS Activation 42 redefinition de 14 classes pr6cedemment 6tablies. CD96 TACTILE Activation 160 Les adjonctions et les modifications apport6es a CD97 Activation 74,80,89 la nomenclature existante des CD sont r6sum6es CD98 4F2, 2F3 T Cell 80,40 dans un tableau.

808 WHO Bulletin OMS. Vol 72 1994