Table S1. Patient Characteristics of the Validation Set

Table S1. Patient characteristics of the validation set

AB0i (n = 18) HLAi (n = 9) P

Type of donor, n (deceased, living) 0/18 9/0 < 0.001

Retransplantation, n (1st/2nd/ 3rd) 18/0/0 2/5/2/0 < 0.001 Recipient age, years 47 [24;68] 45 [21;78] 0.817 Recipient sex, male, n 11 8 0.757 Donor age, years 45 [25;74] 46 [23;60] 0.872 Donor sex, male, n 8 3 0.692 Peak PRA 0 [0;92] 50 [0;96] 0.012 HLA mismatch 3 [2;6] 4[2;5] 0.170 Dialysis vintage (months) 5.5 [0;62] 46.8 [4;92.4] 0.008 Cold ischemia, hours 0.6 [0;2.3] 17.4 [9;21.6] < 0.001

Antibodies before transplantation

Hemagglutinins, titers 1:16 [1:2;1:128]

DSA class I, n 0 5 0 DSA class I, MFI 2000 [0;15300]

DSA class II, n 1 3 0 DSA class II, MFI 2000 950 [0;22300]

Antibodies after transplantation

Hemagglutinins, titers 1:2 [1:1;1:8]

DSA class I, n 0 4

DSA class I, MFI 0 [0; 11 800]

DSA class II, n 0 3

DSA class II, MFI 0 [0; 5000]

Induction treatment < 0.001

Basiliximab, n 16 1

Thymoglobuline, n 2* 8

Serum creatinine at 3 months (µmol/l) 105 [72;146] 138 [88;172] 0.043

Data are presented as n or medians [min; max]. *Two patients from the validation AB0i cohort received rATG induction due to increased risk of rejection (peak PRA 63 %, DSA of 2000 MFI)

Table S2. Histological scores of analyzed biopsies in the validation set of patients.

Banff score AB0i (n = 18) HLAi (n = 9) P valuep (0/1/2/3) g 14/1/1/0 5/3/0/1 0.124 cg 16/0/0/0 6/1/1/0 0.113 mm 16/0/0/0 9/0/0/0 1 i 16/0/1/0 7/1/0/0 0.176 t 12/2/3/0 5/2/1/0 0.691 ti 13/4/0/0 4/3/1/0 0.116 ci 6/11/0/0 4/4/0/0 0.484 ct 4/13/0/0 1/7/0/0 0.520 v 16/0/0/0 8/0/0/0 0.746 cv 0/12/3/1 1/4/2/0 0.609 ah 3/7/6/1 2/3/3/0 0.886 ptc-s 16/0/1/0 5/3/1/0 0.031 ptc-q 16/1/0/0 5/2/2/0 0.045 ptc-e 16/1/0/0 6/2/1/0 0.151 C4d 0/3/2/12 0/2/1/6 0.961

Table S3. GO terms enriched in controls compared to HLAi or AB0i patients. Genes with higher expression in controls compared either to the AB0i or HLAi group (FC > 2, p < 0.05) were entered into the David gene ontology database.

GO terms upregulated in controls compared Count Benjamini p to the AB0i group value GO:0006952 defense response 33 1.44E-06

GO:0009611 response to wounding 28 3.15E-05

GO:0006954 inflammatory response 21 6.98E-05

GO:0006955 immune response 29 2.94E-04

GO:0050778 positive regulation of the immune response 13 7.49E-04

GO:0002684 positive regulation of the immune system 16 1.13E-03 process GO:0002253 activation of the immune response 10 2.94E-03

GO:0002526 acute inflammatory response 10 3.07E-03

GO:0006959 humoral immune response 9 4.50E-03

GO:0006956 complement activation 7 5.26E-03

GO terms upregulated in controls compared Count Benjamini p to the HLAi group value GO:0005578 proteinaceous extracellular matrix 46 7.95E-14

GO:0031012 extracellular matrix 48 1.59E-13

GO:0044420 extracellular matrix part 25 1.37E-10

GO:0005576 extracellular region 123 7.76E-10

GO:0044421 extracellular region part 74 5.70E-09

GO:0005581 collagen 11 1.25E-05

GO:0022610 biological adhesion 54 1.99E-05

GO:0031226 intrinsic to plasma membrane 76 2.73E-05

GO:0007155 cell adhesion 54 3.77E-05

GO:0030198 extracellular matrix organization 18 3.90E-05

GO:0005604 basement membrane 14 9.16E-05

GO:0005887 integral to plasma membrane 72 1.45E-04 GO:0043062 extracellular structure organization 21 2.80E-04

GO:0005201 extracellular matrix structural constituent 15 4.63E-04

GO:0030199 collagen fibril organization 9 1.19E-03

GO:0001501 skeletal system development 29 1.28E-03

GO:0044459 plasma membrane part 109 1.66E-03

GO:0008201 heparin binding 15 2.16E-03

GO:0005539 glycosaminoglycan binding 17 2.42E-03

GO:0030247 polysaccharide binding 18 2.58E-03

GO:0001871 pattern binding 18 2.58E-03

GO:0030934 anchoring collagen 5 5.28E-03

KEGG PATHWAY hsa04512:ECM-receptor interaction 17 1.21E-06

KEGG PATHWAY hsa04510:focal adhesion 21 4.61E-04

GO terms upregulated in the HLAi group Count Benjamini p compared to controls value GO:0055114 oxidation reduction 59 6.92E-05

GO:0048037 cofactor binding 32 2.29E-04

GO:0016054 organic acid catabolic process 20 3.66E-04

GO:0046395 carboxylic acid catabolic process 20 3.66E-04

GO:0006811 ion transport 64 4.53E-04

GO:0000267 cell fraction 81 1.14E-03

GO:0006631 fatty acid metabolic process 26 1.26E-03

GO:0006812 cation transport 49 1.46E-03

GO:0005624 membrane fraction 62 2.31E-03

GO:0016323 basolateral plasma membrane 24 2.43E-03

GO:0009063 cellular amino acid catabolic process 14 2.46E-03

GO:0016324 apical plasma membrane 19 2.60E-03

GO:0050662 coenzyme binding 24 2.73E-03

GO:0005626 insoluble fraction 64 3.04E-03 GO:0045177 apical part of cell 23 3.26E-03

GO:0015293 symporter activity 20 3.89E-03

GO:0055085 transmembrane transport 48 4.22E-03

GO:0006820 anion transport 20 4.79E-03

GO:0005506 iron ion binding 31 5.96E-03

GO:0009055 electron carrier activity 25 7.44E-03

GO:0044271 nitrogen compound biosynthetic process 32 7.83E-03

GO:0009310 amine catabolic process 14 7.92E-03

GO:0006732 coenzyme metabolic process 20 8.12E-03

GO:0009410 response to xenobiotic stimulus 8 8.71E-03

GO:0015695 organic cation transport 8 8.71E-03

KEGG PATHWAY hsa00982:drug metabolism 15 1.53E-04

KEGG PATHWAY hsa00980:metabolism of xenobiotics by 15 2.00E-04 cytochrome P450 KEGG PATHWAY hsa00053:ascorbate and aldarate metabolism 8 5.82E-04

KEGG PATHWAY hsa00480:glutathione metabolism 12 1.31E-03

KEGG PATHWAY hsa00500:starch and sucrose metabolism 10 5.75E-03

KEGG PATHWAY hsa00040:pentose and glucuronate 7 6.17E-03 interconversions

*Only GO terms with Benjamini p values < 0.01 are shown.

Table S4. Upregulated genes for 3-month protocol C4d-positive biopsies of AB0i compared to HLAi patients.

Illumina Array Rank Entrez Gene ID Gene Symbol Fold change P value Address ID 1 5690343 57449 PLEKHG5 6.37 0.0000 2 670068 1571 CYP2E1 6.27 0.0106 3 3400184 137902 PXDNL 6.23 0.0002 4 6550204 84870 RSPO3 5.52 0.0028 5 5550414 56475 RPRM 4.98 0.0009 6 6960561 148808 MFSD4 4.97 0.0004 7 2650382 4626 MYH8 4.87 0.0164 8 2230475 284835 C21orf130 4.74 0.0003 9 4560647 348932 SLC6A18 4.37 0.0482 10 4210719 8092 CART1 4.36 0.0014 11 730563 650263 LOC650263 4.36 0.0047 12 6040020 NA 4.27 0.0019 13 7610196 89792 GAL3ST3 4.17 0.0004 14 2030095 NA 4.16 0.0022 15 770736 338328 GPIHBP1 4.15 0.0003 16 7200328 NA 4.10 0.0020 17 3060273 4504 MT3 3.94 0.0004 18 3440541 728816 LOC728816 3.89 0.0001 19 10131 2911 GRM1 3.88 0.0025 20 380019 115265 DDIT4L 3.78 0.0054 21 7320669 25891 PAMR1 3.72 0.0247 22 5560674 4023 LPL 3.69 0.0017 23 2480427 NA 3.68 0.0001 24 1470053 57221 KIAA1244 3.67 0.0002 25 6590538 7018 TF 3.66 0.0068 26 4760390 83539 CHST9 3.63 0.0015 27 1230477 10265 IRX5 3.63 0.0043 28 4920102 148808 MFSD4 3.54 0.0003 29 4560523 27129 HSPB7 3.53 0.0002 30 4810142 2103 ESRRB 3.49 0.0009 31 1690050 41 ACCN2 3.47 0.0200 32 3360500 1950 EGF 3.46 0.0005 33 6650504 24 ABCA4 3.45 0.0008 34 2680056 1066 CES1 3.41 0.0008 35 4180324 115572 FAM46B 3.40 0.0016 36 6100259 7447 VSNL1 3.39 0.0070 37 360402 273 AMPH 3.39 0.0037 38 6620035 845 CASQ2 3.34 0.0023 39 5360301 55885 LMO3 3.33 0.0011 40 7100722 157724 SLC7A13 3.28 0.0312 41 7610193 26223 FBXL21 3.25 0.0013 42 1690561 10777 ARPP-21 3.24 0.0064 43 5290026 114800 CCDC85A 3.22 0.0015 44 4760040 4703 NEB 3.17 0.0005 45 7650068 349136 WDR86 3.15 0.0025 46 2570746 26153 KIF26A 3.13 0.0000 47 6510403 51557 LGSN 3.13 0.0264 48 6980064 654 BMP6 3.11 0.0059 49 4220592 55799 CACNA2D3 3.11 0.0017 50 6650296 649044 LOC649044 3.08 0.0004 51 5310722 2047 EPHB1 3.06 0.0011 52 6350397 55107 ANO1 3.03 0.0043 53 5810307 130576 LYPD6B 3.02 0.0007 54 5390687 1363 CPE 3.02 0.0020 55 4250301 150244 FLJ31568 3.01 0.0201 56 3190121 130576 LYPD6B 2.99 0.0002 57 5050390 9066 SYT7 2.99 0.0010 58 1740630 2813 GP2 2.97 0.0068 59 3850112 116362 RBP7 2.96 0.0003 60 7000427 5733 PTGER3 2.96 0.0053 61 2340347 23287 AGTPBP1 2.95 0.0020 62 1030286 6523 SLC5A1 2.94 0.0299 63 6650438 9145 SYNGR1 2.93 0.0002 64 2750563 5502 PPP1R1A 2.93 0.0011 65 1070689 202151 RANBP3L 2.92 0.0015 66 2900154 6557 SLC12A1 2.89 0.0053 67 4280739 147495 APCDD1 2.87 0.0209 68 4200754 6484 ST3GAL4 2.85 0.0020 69 780341 83643 CCDC3 2.85 0.0082 70 3440376 2235 FECH 2.85 0.0030 71 5360670 5328 PLAU 2.85 0.0011 72 5390128 55203 LGI2 2.84 0.0141 73 7000433 6344 SCTR 2.84 0.0048 74 4230739 57537 SORCS2 2.84 0.0080 75 6420025 55107 TMEM16A 2.83 0.0032 76 6480433 1288 COL4A6 2.82 0.0013 77 60603 2743 GLRB 2.81 0.0021 78 870575 63876 PKNOX2 2.77 0.0003 79 2100228 1805 DPT 2.73 0.0143 80 5570682 79827 ASAM 2.73 0.0153 81 7100154 783 CACNB2 2.72 0.0002 82 6980543 4004 LMO1 2.71 0.0003 83 5050561 79781 IQCA1 2.69 0.0007 84 3840376 84913 ATOH8 2.69 0.0025 85 3840753 23462 HEY1 2.69 0.0051 86 1660019 9627 SNCAIP 2.68 0.0203 87 2750431 6261 RYR1 2.66 0.0142 88 4810468 6403 SELP 2.65 0.0214 89 3870647 9270 ITGB1BP1 2.62 0.0160 90 6420630 51435 SCARA3 2.62 0.0214 91 1300470 256691 MAMDC2 2.62 0.0044 92 3780221 643911 LOC643911 2.61 0.0045 93 6400274 55107 TMEM16A 2.60 0.0070 94 7050315 148808 MFSD4 2.59 0.0187 95 3870246 4915 NTRK2 2.59 0.0043 96 4290037 4856 NOV 2.59 0.0068 97 620349 4915 NTRK2 2.59 0.0013 98 6200333 6581 SLC22A3 2.58 0.0035 99 1430377 79785 RERGL 2.58 0.0015 100 4860743 163933 FAM43B 2.58 0.0010 101 6650280 114905 C1QTNF7 2.57 0.0021 102 7150762 5740 PTGIS 2.57 0.0317 103 5080259 3790 KCNS3 2.56 0.0015 104 5890091 78986 DUSP26 2.55 0.0079 105 110706 10278 EFS 2.55 0.0003 106 2850451 2322 FLT3 2.55 0.0241 107 4260554 6493 SIM2 2.54 0.0142 108 3310022 10265 IRX5 2.54 0.0049 109 2900286 100134444 LOC100134444 2.53 0.0064 110 1430682 NA 2.51 0.0002 111 4040246 2500 FTHL7 2.51 0.0127 112 6450129 9452 ITM2A 2.50 0.0011 113 5260291 5443 POMC 2.49 0.0149 114 4610424 5950 RBP4 2.49 0.0115 115 5690408 79191 IRX3 2.48 0.0107 116 1990661 138255 C9orf135 2.48 0.0080 117 2370064 432 ASGR1 2.46 0.0410 118 360379 7432 VIP 2.46 0.0104 119 830491 90161 HS6ST2 2.45 0.0011 120 1090768 5019 OXCT1 2.44 0.0002 121 6270274 3306 HSPA2 2.44 0.0033 122 4860441 26167 PCDHB5 2.43 0.0030 123 4810487 55531 ELMOD1 2.43 0.0207 124 2340187 140469 MYO3B 2.43 0.0032 125 6200148 5121 PCP4 2.43 0.0096 126 3850133 285556 LOC285556 2.42 0.0013 127 6420470 200150 PLD5 2.42 0.0016 128 620626 10 NAT2 2.41 0.0168 129 5420037 5136 PDE1A 2.41 0.0056 130 1740451 7850 IL1R2 2.41 0.0013 131 6380040 1298 COL9A2 2.40 0.0017 132 1660050 1852 DUSP9 2.40 0.0014 133 7550750 388556 LOC388556 2.39 0.0045 134 3800215 9721 KIAA0514 2.38 0.0047 135 1440703 149461 CLDN19 2.37 0.0020 136 6330504 56256 SERTAD4 2.37 0.0108 137 6660707 5101 PCDH9 2.37 0.0001 138 2940577 3306 HSPA2 2.36 0.0075 139 2190167 7552 ZNF711 2.35 0.0069 140 7330215 136306 SVOPL 2.35 0.0233 141 7100136 54626 HES2 2.34 0.0417 142 5860689 5625 PRODH 2.34 0.0474 143 4070048 261734 NPHP4 2.34 0.0109 144 4560088 439949 LOC439949 2.34 0.0025 145 6840519 23624 CBLC 2.33 0.0126 146 5720523 150 ADRA2A 2.32 0.0023 147 2760544 80714 PBX4 2.31 0.0046 148 3520017 100129346 LOC100129346 2.31 0.0038 149 620017 406998 MIR216A 2.31 0.0166 150 4640576 5588 PRKCQ 2.31 0.0016 151 6100494 90161 HS6ST2 2.30 0.0039 152 3800010 7373 COL14A1 2.29 0.0289 153 5290014 169026 SLC30A8 2.28 0.0460 154 5490040 10242 KCNMB2 2.27 0.0042 155 2000270 91862 MARVELD3 2.27 0.0014 156 2940538 83987 CCDC8 2.27 0.0060 157 2320358 64116 SLC39A8 2.26 0.0212 158 580148 7461 CYLN2 2.26 0.0102 159 430102 4915 NTRK2 2.26 0.0104 160 6620017 283078 MKX 2.25 0.0147 161 2690437 114784 CSMD2 2.25 0.0031 162 3610102 2307 FOXS1 2.25 0.0326 163 3930446 8437 RASAL1 2.24 0.0010 164 460364 3400 ID4 2.24 0.0177 165 4060209 643911 LOC643911 2.24 0.0081 166 6840220 130574 LYPD6 2.24 0.0026 167 4560301 10777 ARPP-21 2.23 0.0324 168 5900376 64881 PCDH20 2.23 0.0189 169 1740161 55740 ENAH 2.22 0.0171 170 5550440 6529 SLC6A1 2.22 0.0433 171 70224 283238 MGC34821 2.21 0.0205 172 6100333 652634 LOC652634 2.21 0.0011 173 20100 11174 ADAMTS6 2.21 0.0420 174 6100598 9478 CABP1 2.21 0.0096 175 540204 3790 KCNS3 2.21 0.0002 176 4290739 100128516 LOC100128516 2.21 0.0016 177 430743 744 MPPED2 2.20 0.0004 178 4070450 340267 COL28A1 2.20 0.0330 179 5860519 1464 CSPG4 2.20 0.0096 180 5080196 NA 2.19 0.0005 181 1470176 51760 SYT17 2.19 0.0080 182 270152 8140 SLC7A5 2.18 0.0088 183 3440164 642469 LOC642469 2.17 0.0032 184 160445 8490 RGS5 2.17 0.0011 185 6220309 23416 KCNH3 2.17 0.0336 186 4480731 51285 RASL12 2.16 0.0150 187 1260180 2070 EYA4 2.15 0.0045 188 1430634 339541 MGC33556 2.15 0.0477 189 4610451 6299 SALL1 2.15 0.0305 190 6100095 2642 GCGR 2.14 0.0041 191 4730088 286097 EFHA2 2.14 0.0069 192 2850279 730077 LOC730077 2.14 0.0136 193 6770369 7369 UMOD 2.14 0.0014 194 3370162 10418 SPON1 2.14 0.0238 195 4040341 146894 CD300LG 2.14 0.0146 196 5130059 6450 SH3BGR 2.13 0.0003 197 2510682 645146 LOC645146 2.13 0.0187 198 460504 1238 CCBP2 2.13 0.0251 199 2940594 846 CASR 2.12 0.0051 200 4060605 118812 MORN4 2.12 0.0009 201 4560433 256309 CCDC110 2.12 0.0185 202 5570520 6236 RRAD 2.11 0.0181 203 6590735 57523 NYNRIN 2.11 0.0010 204 3460037 84735 CNDP1 2.10 0.0236 205 2030403 116448 OLIG1 2.10 0.0197 206 2680441 5733 PTGER3 2.10 0.0005 207 1050176 8521 GCM1 2.10 0.0157 208 6290008 56131 PCDHB4 2.10 0.0331 209 150291 90317 ZNF616 2.09 0.0027 210 2120379 827 CAPN6 2.09 0.0076 211 7000494 145773 FAM81A 2.09 0.0077 212 7150598 2947 GSTM3 2.08 0.0005 213 2470088 10144 FAM13A 2.08 0.0019 214 4390537 79582 SPAG16 2.08 0.0110 215 3520020 26999 CYFIP2 2.07 0.0001 216 1660538 27098 CLUL1 2.07 0.0163 217 1170553 7447 VSNL1 2.07 0.0463 218 6480170 202151 RANBP3L 2.07 0.0058 219 670707 199964 TMEM61 2.07 0.0070 220 2850458 783 CACNB2 2.06 0.0202 221 5670154 129804 FBLN7 2.06 0.0010 222 4850370 81029 WNT5B 2.06 0.0003 223 6250619 541468 C1orf190 2.06 0.0109 224 6840390 146330 FBXL16 2.05 0.0147 225 4760164 27433 TOR2A 2.05 0.0008 226 730358 56967 C14orf132 2.05 0.0178 227 2760398 80863 PRRT1 2.04 0.0166 228 3930403 1909 EDNRA 2.04 0.0133 229 580187 8622 PDE8B 2.04 0.0268 230 3420301 64924 SLC30A5 2.03 0.0021 231 1440709 8483 CILP 2.03 0.0312 232 4640315 140689 CBLN4 2.03 0.0310 233 6350477 283683 LOC283683 2.03 0.0423 234 1470431 5152 PDE9A 2.03 0.0033 235 6380239 84251 SGIP1 2.02 0.0324 236 6580477 NA 2.02 0.0129 237 1820414 479 ATP12A 2.02 0.0254 238 1450202 81621 KAZALD1 2.02 0.0228 239 1400259 81558 FAM117A 2.02 0.0033 240 360082 84901 NFATC2IP 2.01 0.0008 241 6860180 728216 LOC728216 2.01 0.0132 242 6520242 100129975 LOC100129975 2.01 0.0095 243 2600528 169355 IDO2 2.01 0.0412 244 5570768 221883 HOXA11AS 2.01 0.0102 245 6900349 27098 CLUL1 2.01 0.0093 246 6960022 5413 SEPT5 2.01 0.0014 247 3840553 388815 C21orf34 2.01 0.0000 248 6100072 27147 DENND2A 2.01 0.0241 249 6980632 114783 LMTK3 2.01 0.0265 250 1470296 3908 LAMA2 2.01 0.0122 251 3450369 374650 FLJ40113 2.00 0.0003 *Genes selected for RT-qPCR validation are displayed in bold.

Table S5. Downregulated genes for 3-month protocol C4d positive biopsies of AB0i compared to HLAi patients.

Illumina Array Rank Entrez Gene ID Gene symbol Fold change P value Address ID 1 2260326 6690 SPINK1 19.03 0.000744 2 2320524 9376 SLC22A8 14.89 0.009237 3 620593 7827 NPHS2 13.66 0.005395 4 3610600 338094 FAM151A 11.09 0.022412 5 6280369 338094 FAM151A 9.46 0.011933 6 6020739 7780 SLC30A2 9.32 0.005641 7 6180711 153201 SLC36A2 9.09 0.030235 8 4810445 26577 PCOLCE2 8.34 0.005398 9 1010746 79625 C4orf31 8.32 0.00253 10 4640240 793 CALB1 7.94 0.01856 11 780768 5972 REN 6.82 0.01732 12 2900725 1594 CYP27B1 6.57 0.00765 13 2140543 4868 NPHS1 6.46 0.00428 14 5310646 57016 AKR1B10 6.43 0.02334 15 3190746 5053 PAH 6.41 0.01474 16 1070243 6559 SLC12A3 6.26 0.01471 17 670736 3242 HPD 6.15 0.04057 18 6580110 5053 PAH 5.95 0.03519 19 4860537 23428 SLC7A8 5.92 0.00997 20 6020300 9173 IL1RL1 5.86 0.00297 21 6510068 1409 CRYAA 5.80 0.04267 22 5420754 9173 IL1RL1 5.76 0.00408 23 6020132 53405 CLIC5 5.73 0.00403 24 3610019 92126 DSEL 5.62 0.00019 25 5870672 29947 DNMT3L 5.44 0.01640 26 1230129 23109 DDN 5.40 0.00532 27 1440537 50964 SOST 5.27 0.01989 28 4060427 3026 HABP2 5.25 0.01096 29 6020139 5650 KLK7 5.17 0.00204 30 6400131 1591 CYP24A1 5.14 0.04277 31 5890452 6524 SLC5A2 5.13 0.01794 32 6590376 6514 SLC2A2 5.10 0.02252 33 130128 285016 LOC285016 5.00 0.00142 34 5420386 3598 IL13RA2 4.99 0.00367 35 1410465 54600 UGT1A9 4.99 0.00367 36 380259 3620 INDO 4.93 0.02740 37 6940270 56667 MUC13 4.91 0.04286 38 2260324 285023 DKFZp451M2119 4.90 0.00259 39 1170242 100129165 LOC100129165 4.86 0.00262 40 7210240 2244 FGB 4.86 0.02871 41 1070088 54575 UGT1A10 4.85 0.00707 42 7510132 4973 OLR1 4.82 0.00213 43 1580561 4496 MT1H 4.81 0.04679 44 5260743 NA 4.79 0.00725 45 5340685 9356 SLC22A6 4.79 0.03076 46 1340408 54577 UGT1A7 4.70 0.01080 47 1230170 6524 SLC5A2 4.61 0.00590 48 3940541 5800 PTPRO 4.59 0.01291 49 5290630 2244 FGB 4.57 0.00869 50 520523 4051 CYP4F3 4.48 0.00734 51 4390333 22854 NTNG1 4.47 0.01246 52 6960484 3167 HMX2 4.35 0.00525 53 2970154 5653 KLK6 4.33 0.03505 54 1690184 25897 RNF19A 4.28 0.00014 55 5260592 127124 ATP6V1G3 4.14 0.00642 56 780619 9356 SLC22A6 4.12 0.03068 57 2190524 138065 RNF183 4.02 0.00436 58 5570711 3620 IDO1 4.01 0.01419 59 2100706 114815 SORCS1 3.99 0.00899 60 60039 7780 SLC30A2 3.98 0.00617 61 7650161 50507 NOX4 3.97 0.01238 62 3610170 5967 REG1A 3.92 0.03298 63 4120392 84647 PLA2G12B 3.90 0.01868 64 3420291 391123 VSIG8 3.85 0.00940 65 6290192 51402 HSFX1 3.84 0.04375 66 4810292 1380 CR2 3.84 0.00415 67 3180243 29947 DNMT3L 3.84 0.01879 68 6480382 6568 SLC17A1 3.83 0.01895 69 5700390 83697 SLC4A9 3.83 0.00559 70 10066 10786 SLC17A3 3.81 0.00940 71 6840138 729960 LOC729960 3.81 0.02790 72 6380494 22925 PLA2R1 3.77 0.00075 73 6420736 56521 DNAJC12 3.75 0.01004 74 5360064 10578 GNLY 3.75 0.04689 75 2120152 6521 SLC4A1 3.75 0.00286 76 2680332 63928 CHP2 3.72 0.00055 77 1340021 10551 AGR2 3.71 0.00333 78 1070121 340274 LOC340274 3.69 0.01426 79 3890093 2880 GPX5 3.57 0.01962 80 5220524 51440 HPCAL4 3.56 0.01133 81 2570575 285016 FAM150B 3.56 0.00564 82 6380672 762 CA4 3.55 0.01294 83 1340348 2880 GPX5 3.53 0.02157 84 5960162 127124 ATP6V1G3 3.50 0.01842 85 5900152 50507 NOX4 3.47 0.01722 86 6180228 115352 FCRL3 3.46 0.00479 87 4260333 3572 IL6ST 3.45 0.00020 88 1400634 4499 MT1M 3.45 0.00073 89 5720075 8516 ITGA8 3.43 0.00513 90 2030100 NA 3.42 0.00711 91 5390669 2562 GABRB3 3.35 0.00098 92 4850674 29968 PSAT1 3.35 0.01854 93 2690414 1393 CRHBP 3.33 0.00342 94 130093 4496 MT1H 3.33 0.03310 95 5080279 123264 OSTbeta 3.32 0.02799 96 3420288 10630 PDPN 3.30 0.00402 97 4570220 100130422 LOC100130422 3.30 0.00000 98 160142 51463 GPR89A 3.28 0.00029 99 5820193 7301 TYRO3 3.28 0.01013 100 2340682 127933 UHMK1 3.20 0.00011 101 7330376 159963 SLC5A12 3.18 0.01505 102 6040451 146802 SLC47A2 3.18 0.02235 103 2260504 100132395 LOC100132395 3.17 0.00043 104 1030142 51458 RHCG 3.16 0.03279 105 1820451 27333 GOLIM4 3.15 0.00047 106 5490692 26470 SEZ6L2 3.15 0.00428 107 6180288 5788 PTPRC 3.15 0.01438 108 4560398 648639 LOC648639 3.15 0.00124 109 1660386 5800 PTPRO 3.14 0.02389 110 1190091 10655 DMRT2 3.14 0.00394 111 6100168 27333 GOLPH4 3.13 0.00114 112 6900538 56521 DNAJC12 3.13 0.01637 113 4290465 253012 HEPACAM2 3.12 0.02418 114 4220278 6738 TROVE2 3.11 0.00038 115 2450411 56521 DNAJC12 3.11 0.03817 116 7150521 53345 TM6SF2 3.10 0.00217 117 1050747 10631 POSTN 3.07 0.00469 118 1940747 84152 PPP1R1B 3.04 0.01146 119 4670719 80829 ZFP91 3.04 0.00130 120 2450725 6520 SLC3A2 3.04 0.00998 121 5130553 116449 MIST 3.03 0.00136 122 1580086 130120 REG3G 3.01 0.02042 123 1940450 8496 PPFIBP1 3.00 0.00248 124 4560673 392871 LOC392871 2.96 0.03071 125 6770358 378465 HSN2 2.95 0.00006 126 2320273 92840 REEP6 2.95 0.04895 127 110653 80896 NPL 2.95 0.00758 128 5310754 8876 VNN1 2.94 0.01832 129 7380603 7490 WT1 2.94 0.00288 130 1820279 90139 TSPAN18 2.93 0.02135 131 2760274 441282 AKR1B15 2.93 0.03944 132 460484 25819 CCRN4L 2.92 0.01088 133 6940601 56142 PCDHA6 2.91 0.01254 134 2370070 440352 RUNDC2C 2.91 0.00033 135 1580128 2700 GJA3 2.91 0.00698 136 2640161 3275 PRMT2 2.90 0.00072 137 3170592 56886 UGCGL1 2.89 0.00137 138 1230524 256435 ST6GALNAC3 2.89 0.01441 139 1570544 6492 SIM1 2.89 0.00090 140 6580041 10578 GNLY 2.88 0.03894 141 540243 80896 NPL 2.87 0.02771 142 510246 10631 POSTN 2.86 0.01759 143 6400356 283431 GAS2L3 2.85 0.00007 144 620239 1827 RCAN1 2.85 0.01368 145 780037 100129165 LOC100129165 2.85 0.01156 146 7380725 2885 GRB2 2.84 0.00078 147 4640324 116085 SLC22A12 2.83 0.02421 148 2970575 388372 CCL4L2 2.82 0.03777 149 1570300 221178 SPATA13 2.81 0.02809 150 1050561 27329 ANGPTL3 2.80 0.02986 151 4540468 149563 C1orf64 2.80 0.01591 152 6480427 79858 NEK11 2.79 0.00005 153 270452 27072 VPS41 2.78 0.00050 154 6480240 402560 LOC402560 2.77 0.00064 155 6220687 51196 PLCE1 2.76 0.01074 156 6590682 6348 CCL3 2.75 0.00329 157 3840564 58475 MS4A7 2.75 0.01252 158 1170332 22848 AAK1 2.75 0.02754 159 2510091 1295 COL8A1 2.74 0.01055 160 6270553 3627 CXCL10 2.74 0.04051 161 4590392 401250 MCCD1 2.73 0.00291 162 2100215 4094 MAF 2.72 0.00132 163 1190739 162073 ITPRIPL2 2.71 0.01979 164 150100 6274 S100A3 2.71 0.00943 165 2000286 83735 C8orf16 2.71 0.00080 166 7320682 5106 PCK2 2.71 0.01819 167 2690431 128710 C20orf94 2.70 0.01279 168 6560201 728835 LOC728835 2.69 0.04494 169 4730066 6326 SCN2A 2.68 0.00001 170 6620440 NA 2.67 0.00337 171 2680131 8496 PPFIBP1 2.66 0.00404 172 3850221 92126 DSEL 2.66 0.01519 173 4860538 728912 NBPF11 2.64 0.00052 174 3170274 114882 OSBPL8 2.64 0.00166 175 5130053 8671 SLC4A4 2.63 0.00303 176 3520102 9560 CCL4L1 2.62 0.03903 177 4590619 10687 PNMA2 2.62 0.00225 178 6220403 5062 PAK2 2.61 0.02340 179 5670674 4311 MME 2.60 0.03008 180 5260307 55607 PPP1R9A 2.60 0.00123 181 6180259 26353 HSPB8 2.57 0.00870 182 2350121 642678 LOC642678 2.57 0.00115 183 10026 6023 RMRP 2.57 0.00426 184 6580088 649841 LOC649841 2.57 0.00660 185 3610743 7536 SF1 2.57 0.00406 186 4860747 654342 LOC654342 2.56 0.00073 187 3890288 84676 TRIM63 2.55 0.01877 188 4210356 100130943 LOC100130943 2.55 0.00543 189 6480189 7069 THRSP 2.55 0.03126 190 7650725 56886 UGCGL1 2.55 0.02030 191 50739 22925 PLA2R1 2.53 0.00908 192 3390594 3824 KLRD1 2.53 0.01191 193 4280528 728518 LOC728518 2.53 0.00229 194 5270288 645321 LOC645321 2.52 0.00267 195 7050010 54796 BNC2 2.52 0.00170 196 7550220 6273 S100A2 2.52 0.03795 197 610482 6840 SVIL 2.51 0.00108 198 2630176 4137 MAPT 2.51 0.03454 199 2640349 729960 LOC729960 2.50 0.03797 200 7050082 54 ACP5 2.49 0.03525 201 2650093 10725 NFAT5 2.48 0.00896 202 2850703 2243 FGA 2.48 0.04126 203 3850189 26834 RNU4-2 2.48 0.00065 204 5820673 114815 SORCS1 2.47 0.00748 205 6620528 4501 MT1X 2.47 0.02142 206 620603 NA 2.47 0.00365 207 110433 445 ASS1 2.47 0.03549 208 7000259 10521 DDX17 2.47 0.00042 209 5220093 84868 HAVCR2 2.46 0.00569 210 3460086 54434 SSH1 2.46 0.00040 211 2350747 22885 ABLIM3 2.45 0.00852 212 1170300 4495 MT1G 2.44 0.03764 213 6590722 51742 ARID4B 2.44 0.00609 214 5390220 6280 S100A9 2.43 0.00945 215 10681 100131164 LOC100131164 2.43 0.03446 216 7050524 650346 LOC650346 2.42 0.00067 217 5900360 64149 C17orf75 2.42 0.02268 218 990202 9217 VAPB 2.41 0.00037 219 1740433 10943 MSL3L1 2.41 0.01610 220 6510500 23678 SGK3 2.40 0.00013 221 5050600 158295 MGC24103 2.39 0.00721 222 70100 133688 UGT3A1 2.39 0.02090 223 4010039 7139 TNNT2 2.37 0.02881 224 7510008 30845 EHD3 2.37 0.00956 225 3290717 64395 GMCL1 2.36 0.00012 226 1450504 NA 2.36 0.02341 227 5570195 54014 BRWD1 2.36 0.01111 228 3800241 1004 CDH6 2.35 0.00443 229 3400692 7170 TPM3 2.35 0.01201 230 2650047 118471 PRAP1 2.35 0.04219 231 2810010 414062 CCL3L3 2.35 0.04419 232 540671 10288 LILRB2 2.35 0.03896 233 2490564 NA 2.34 0.01060 234 1580048 831 CAST 2.34 0.02160 235 5900132 729222 LOC729222 2.33 0.01169 236 3360452 1295 COL8A1 2.33 0.02618 237 1850504 55294 FBXW7 2.33 0.00543 238 6760209 9774 BCLAF1 2.32 0.00167 239 5290564 11332 ACOT7 2.31 0.04318 240 6220112 619383 SCARNA9 2.31 0.00476 241 4570630 259173 ALS2CL 2.31 0.00910 242 2470730 NA 2.31 0.00018 243 5420491 728689 EIF3CL 2.30 0.00638 244 2340707 2028 ENPEP 2.29 0.03047 245 6220360 8659 ALDH4A1 2.28 0.03783 246 1090521 26470 SEZ6L2 2.28 0.01228 247 5900435 57551 TAOK1 2.27 0.00298 248 4610520 9245 GCNT3 2.27 0.01745 249 7200427 8862 APLN 2.26 0.00114 250 6330576 5420 PODXL 2.25 0.01140 251 1230228 10232 MSLN 2.24 0.02365 252 3450452 23095 KIF1B 2.23 0.00418 253 3710575 647786 LOC647786 2.23 0.02454 254 5290148 84706 GPT2 2.23 0.02236 255 6060468 6279 S100A8 2.23 0.02644 256 6290475 57520 HECW2 2.23 0.01893 257 3830433 NA 2.22 0.00052 258 430332 286410 ATP11C 2.21 0.00079 259 940121 650860 LOC650860 2.21 0.01850 260 6900370 NA 2.21 0.01488 261 2510100 441155 LOC441155 2.20 0.00253 262 620403 400759 LOC400759 2.20 0.04403 263 5270102 152404 IGSF11 2.19 0.02461 264 5420575 6520 SLC3A2 2.19 0.01653 265 3800537 55041 PLEKHB2 2.19 0.00258 266 2640544 445 ASS1 2.19 0.04229 267 2000360 100130000 LOC100130000 2.19 0.00961 268 360482 25832 NBPF14 2.19 0.00097 269 2760537 644314 MTE 2.18 0.03636 270 2260176 153443 SRFBP1 2.18 0.00010 271 1070286 4074 M6PR 2.18 0.00738 272 20356 349152 DPY19L2P2 2.17 0.00384 273 5360347 1728 NQO1 2.17 0.00385 274 670301 727851 RGPD8 2.17 0.01750 275 2230746 1244 ABCC2 2.17 0.03435 276 6560328 63027 C6orf85 2.16 0.04141 277 4210164 64081 PBLD 2.16 0.03284 278 1770037 6711 SPTBN1 2.16 0.00877 279 6560358 2246 FGF1 2.15 0.01665 280 4040022 942 CD86 2.15 0.04285 281 4560592 9353 SLIT2 2.14 0.01324 282 630228 348158 ACSM2B 2.14 0.01502 283 2070402 10800 CYSLTR1 2.14 0.02696 284 2060338 203111 C8orf47 2.13 0.01141 285 6200187 126282 TNFAIP8L1 2.13 0.00026 286 6420605 10537 UBD 2.13 0.02188 287 2120048 4883 NPR3 2.13 0.02006 288 4780437 287 ANK2 2.13 0.02069 289 1090390 4938 OAS1 2.13 0.00477 290 650112 7940 LST1 2.13 0.03501 291 5560328 10991 SLC38A3 2.12 0.03130 292 6480484 55966 AJAP1 2.12 0.00400 293 1090739 3987 LIMS1 2.11 0.01273 294 4670561 100133692 LOC100133692 2.11 0.01286 295 4060347 9615 GDA 2.11 0.00697 296 6510170 3437 IFIT3 2.11 0.00663 297 6420019 199731 CADM4 2.10 0.01739 298 1110291 158038 LINGO2 2.09 0.03272 299 1850092 5420 PODXL 2.08 0.00053 300 4060471 2184 FAH 2.08 0.04351 301 2570500 400618 FLJ37644 2.07 0.01996 302 1230746 79670 ZCCHC6 2.07 0.00633 303 7400133 10659 CUGBP2 2.07 0.01937 304 6200446 1393 CRHBP 2.07 0.04785 305 6980735 NA 2.07 0.02257 306 3710678 8613 PPAP2B 2.07 0.00212 307 4220672 4494 MT1F 2.06 0.01745 308 5090315 1804 DPP6 2.06 0.02314 309 6200402 4489 MT1A 2.06 0.02145 310 2190148 2633 GBP1 2.06 0.04617 311 2450554 3516 RBPJ 2.06 0.00829 312 4540327 6238 RRBP1 2.06 0.00008 313 1090500 645166 LOC645166 2.06 0.00158 314 4290278 7490 WT1 2.05 0.01363 315 1300719 112849 C14orf149 2.05 0.02137 316 5080685 5519 PPP2R1B 2.05 0.00380 317 6290239 525 ATP6V1B1 2.05 0.02735 318 4060010 344887 LOC344887 2.04 0.01789 319 2490360 79411 GLB1L 2.04 0.00104 320 1090431 644779 LOC644779 2.04 0.03113 321 730414 348 APOE 2.04 0.04878 322 1660193 729513 LOC729513 2.03 0.00450 323 2690427 55773 TBC1D23 2.03 0.02572 324 6940608 84812 PLCD4 2.03 0.03293 325 5310048 23451 SF3B1 2.02 0.02577 326 770475 NA 2.02 0.00100 327 450082 6300 MAPK12 2.02 0.00620 328 4150369 2710 GK 2.01 0.02241 329 3840053 7360 UGP2 2.01 0.00002 330 1110332 11145 PLA2G16 2.01 0.00196 331 1410598 51231 VRK3 2.01 0.01975 332 4890133 57532 NUFIP2 2.01 0.00568 333 7380138 90843 TCEAL8 2.01 0.00049 334 4560609 55366 LGR4 2.01 0.00317 335 4060064 3161 HMMR 2.01 0.02108 336 7150685 23468 CBX5 2.01 0.00403 337 3400270 57674 RNF213 2.00 0.04378 *Genes selected for RT-qPCR validation are displayed in bold.

Figure S1. Relative numbers of B- and T-cell lymphocyte subsets in the peripheral blood of patients who received their kidney allografts from living donors either after AB0c

(compatible) (n = 44) or AB0i (incompatible) transplantation (n = 9).

Figure S2. Immunohistochemical staining with anti-CD59 and anti-CD46 in C4d negative controls and C4d positive biopsies of AB0i and HLAi patients.