DOCSLIB.ORG

(12) Patent Application Publication (10) Pub. No.: US 2017/0202238 A1 YANNIOS (43) Pub

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
(12) Patent Application Publication (10) Pub. No.: US 2017/0202238 A1 YANNIOS (43) Pub US 20170202238A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2017/0202238 A1 YANNIOS (43) Pub. Date: Jul. 20, 2017 (54) DIETARY SUPPLEMENT COMPOSITIONS (52) U.S. Cl. WITH ENHANCED DELIVERY MATRIX, CPC ............... A23G 3/368 (2013.01); A23G 3/36 GUMMIES, CHOCOLATES, ATOMIZERS (2013.01); A23G 3/48 (2013.01); A23 V AND POWDERS CONTAINING SAME, AND 2002/00 (2013.01) METHODS OF MAKING SAME (71) Applicant: James John YLANNIOS, (57) ABSTRACT PLACENTIA, CA (US) (72) Inventor: James John YLANNIOS, A method of making dietary Supplement compositions PLACENTIA, CA (US) includes generating an aqueous phase (A1) having one or (21) Appl. No.: 15/475,636 more dietary Supplement nutrients (DSN1), generating an oil phase (O1), performing a first homogenizing step by mixing (22) Filed: Mar. 31, 2017 A1 and O1 thereby forming A1/O1 composition, performing a second homogenizing step by mixing the A1/O1 compo Related U.S. Application Data sition and the further added DSN2, performing a third (63) Continuation of application No. 15/414,877, filed on homogenizing step by mixing the A1/O1/DSN2 composition Jan. 25, 2017, which is a continuation-in-part of and a first flavor (F1), performing a fourth homogenizing application No. 14/132,486, filed on Dec. 18, 2013, step by mixing the A1/O1/DSN2/F1 composition and a gum now Pat. No. 9,585,417. dispersed with glycerin (GG), and performing a fifth homog enizing step by mixing the A1/O1/DSN2/F1/GG composi (60) Provisional application No. 61/837.414, filed on Jun. tion and the second flavor (F2) thereby forming dietary 20, 2013. composition A1/O1/DSN2/F1/GG/F2. The A1/O1/DSN2/ F1/GG/F2 composition is dispersion in which actives are Publication Classification incorporated in liposomal vesicles which may have a barrier (51) Int. Cl. coating of a polymer. A method of making gummies includes A23G 3/36 (2006.01) surrounding A1/O1/DSN2/F1/GG/F2 composition (inner A23G 3/48 (2006.01) portion) with an outer portion formed of shell syrup. Patent Application Publication Jul. 20, 2017 Sheet 1 of 17 US 2017/0202238 A1 C C2 DISSOVING SEWA IN 5% W/W OF DSSOLVNGA FIRS MEASUREMENT OF WATER AAPREDEERMNEO CURCUMIN MIX (CM1) IN WATER AT A TEMPERATURE SOAS TO FORM TEMPERATURE NOT MORE THAN 65°C SOAS SUBCOMPOSION-- TO FORMSUBCOMPOSTON C3 ADONG AND/OR MIXING SUBCOMPOSION TO SUBCOMPOSTON-ISO ASO FORM SUBCOMPOSITION-- ADONG AND/OR MXNG SURFACTANT/EMUS FER AND/OR CITRC ACID TO SUBCOMPOSITION-Il, SOAS TO FORMSUBCOMPOSITION-V STRRING SUBCOMPOSITION-W FOR A PREDETERMINED ME PERO SOASO FORM SUBCOMPOSITION-V, i.e., AQUEOUS PHASE (A1) FIG. 1 Patent Application Publication Jul. 20, 2017 Sheet 2 of 17 US 2017/0202238 A1 C6 MIXING PHOSPHOLIPD FRACTION, e.g., SUNFLOWER LECITH IN AND MIXED OCOPHEROS TOGETHER UNDER 65C SIRRING MX OF SUNFOWER ECITH IN AND MIXED TOCOPHEROS FOR ABOUT 2 MINUTES SOASO FORM SUBCOMPOSITION-VI, i.e., OILPHASE (O1) FIG 2 Patent Application Publication Jul. 20, 2017 Sheet 3 of 17 US 2017/0202238 A1 PREPARNG ACUEOUS PHASE A1 ) PREPARING OLPHASE (O1) C8 ADONG O AND A1 IN SANESS SEE WESSE PERFORMING FIRST HOMOGENIZING STEP BY MIXING A1 AND OAT 1,000 RPM PROGRESSIVELY INCREASING TO 5,000 RPM FOR ABOUT 30 MINUTES USINGA HIGH SHEAR HOMOGENZER SOAS TO FORMAFO COMPOSTON CO ADDING ASECOND MEASUREMENT OF CURCUMIN MIX (CM2) TO A1/O1 COMPOSITION Ci PERFORMING SECONO HOMOGENZNG STEP BY MIXING AfC COMPOSION AND CM2A 1,000 RPM PROGRESSIVEY INCREASING TO 5,000 RPM FOR ABOUT 10 MINUTES A & 65°C SOAS TO FORMAOFCM2 COMPOSITON C2 ADDING FIRST FLAVOR (F1) INCLUDING OLPHASE FLAVOR OAFCfCM2 COMPOSION PERFORMING THROHOMOGENIZING SEP BY MXING AfC COMPOSION AND FAT 3000 RPM PROGRESSIVELY INCREASING TO 5,000 FOR ABOUT 3-5 MINUTES AT 65C SOAS TO FORMA OFCM2F COMPOSION C4 ADDING GUM ARABC DISPERSED WITH GLYCERN (GG) TO A?O1ACM2F COMPOSITION PERFORMING FOURTH HOMOGENIZING STEP BY MXNG A?OfCM2/F COMPOSITION AND GG AT ABOUT 1,000 RPMPROGRESSIVELY INCREASING TO ABOUT 5,000 FOR ABOUT MINUTES A & 65 °C SOASO FORMA1/OfCM2FF1/GG COMPOSION C16 ADDING SECOND FLAVOR (F2) INCLUDING A:/O 1/CM2/F1/GG COMPOSION C7 PERFORMING FIFTH HOMOGENZING SEP OF A1/OfCM2/FFGG COMPOSION AND F2ATABOUT 1,000 RPMPROGRESSIVELY NCREASING TO ABOUT 3500 RPM FOR ABOUT OMINUTES A & 65°C SOAS TO FORM AfCfCM2, F1/GG/F2 COMPOSION C8 MATURINGA/O1/CM2/F1/GG/F2 COMPOSITION (DIETARY SUPPLEMENT) FOR 6 -ORS C9 NIROGENFJSHINGAfCACM2FFGGF2 COMPOSITION C20 FIG 3 SPRAY ORYING AfCfCM2AF/CGGAF2 COMPOSITON Patent Application Publication Jul. 20, 2017 Sheet 4 of 17 US 2017/0202238 A1 CP1 CP2 DSSOLVING STEVAN 5% WW OF DSSOLVING FIRST MEASUREMENT OF WAER AAPREDETERMINED CURCUMIN MIX (CM) iN WATER AT A EMPERATURE SOASO FORM EMPERAURE NOT MORE HAN 65C SOAS SUBCOMPOSITION-- TO FORMSUBCOMPOSTON CP3 ADING AND/OR MXING SUBCOMPOSITON-O SUBCOMPOSTION-- SOAS TO FORM SUBCOMPOSION-- CP4 ADDING AND/OR MEXING SURFACANT/EMUSIFIER AND/OR CTRIC ACD TO SUBCOMPOSITION-II, SOAS TO FORMSUBCOMPOSITION-IV CP5 SIRRING SUBCOMPOSITION-W FORA PREDEERMINED TIME PEROD SOAS TO FORM SUBCOMPOSION-V CPS DISSOLVING ONE OF POLYETHYLENE GLYCOL, BOPOLYMER, DERVED FROM FENUGREEK, CHITOSAN, AND A COMBINATION THEREOF, N2% W/W OF WATERA - 45°C, AND ACOING ABOUT 50%. AMOUNT OF GYCER IN TOIT, AND ADDING HYDROLYZED STARCH (ORCYCLODEXTRINSIFACTIVE HAS FOWLSMELLOR TASTE) UNDER STIRRING AND MIXING TOGETHER WEL FOR < 10 MINUTES SOASO FORM SUBCOMPOSTION-V CP7 ADONG AND FOR MXNG SUBCOMPOSTON-VW SUBCOMPOSITION-VI, SOAS TO FORM SUBCOMPOSITION-VII, i.e., AQUEOUS PHASE (A1) FIG 4 Patent Application Publication Jul. 20, 2017 Sheet 5 of 17 US 2017/0202238 A1 CP8 MIXING PHOSPHOLIPID FRACTION, e.g., SUNFLOWER LECITHIN AND MIXED OCOPHEROLS TOGETHER UNDER 65°C CP9 STIRRING MIX OF SUNFLOWER LECI-N AND MIXED TOCOP-EROS FOR ABOU 2 MNUES SOAS TO FORM SUBCOMPOSITION-VII, i.e., OIL PHASE (O1) FIG. 5 Patent Application Publication Jul. 20, 2017 Sheet 6 of 17 US 2017/0202238 A1 CP-CP7 PREPARNG ACRUEOUSPHASE (A) PREPARING O PHASE (O) ADDING O1 AND AN STANLESS STEE WESSEL PERFORMING FIRST HOMOGENZING STEP BY MIXENG A1 AND O1. At 1,000 RPM PROGRESSIVELY INCREASING TO 5,000 RPM FOR ABOUT 30 MINUTES USINGA HIGH SHEAR HOMOGENZEFRSOAS TO FORMA. O.1 COMPOSION CP12 ADDING ASECOND MEASUREMENT OF CURCUMIN MIX (CM2) TO A1/O1 COMPOSITION CP3 PERFORMING SECOND OMOGENIZNG STEP BY MIXNGAAO COMPOSION AND CM2A 1,000 RPM PROGRESSIVELY NCREASING TO 5,000 RPM FOR ABOUT 10 MINUTES AT < 65°C SOASO FORMAFO/CM2 COMPOSION CP1A. ADDING FIRST FLAVOR (F1) INCLUDING OLPHASE FLAVOR CA/OACM2 COMPOSITON CP15 PERFORMING ROOMOGENIZING SEP BY MIXINGAAO ACM2 COMPOSITION AND F A ,000 RPM PROGRESSIVELY INCREASING TO 5,000 FOR ABOUT 3-5 VENUES AT 65C SOASO FORMA OfCV2FF COMPOSION CP16 ADDING GUMARABC DISPERSED WITH GLYCERIN (GG) TO AFOfCM2/F COMPOSITION CP17 PERFORMING FOURTH HOMOGENZING SEP BY MXING A. OfCM2/F COMPOSITION AND GG AND/OR GLYCERIN AT ABOUT 1,000 RPM PROGRESSIVELY INCREASING TO ABOUT 5,000 FOR ABOT 10 MINUTES AT < 65C SOAS TO FORM AfCFCM2F1 GG COMPOSION CP8 ADDING SECOND FLAVOR (F2) INCLUDING AQUEOUS PHASE FLAVOR TO AfC1/CM2F1/GG COMPOSION CP 19 PERFORMING FIFTH HOMOGENZING SEP OF A1/OfCM2/F COMPOSION AND F2 ATABOUT 1,000 RPM PROGRESSIVELY INCREASING TO ABOUT 3500 RPM FOR ABOUT OMNUES A & 65C SOASO FORMAFOfCM2FFAGGF2 COMPOSION CP20 MATURING A1/O1 JCM2/Fi/GG/F2 COMPOSITION (DIETARY SUPPLEMENT) FOR6 HORS CP21 NROGEN FRSNGAfC/CM2, FAGGFF2 COMPOSION CP22 FI G 6 SPRAYDRYING AfCfCM2FAGG,F2 COMPOSION Patent Application Publication Jul. 20, 2017 Sheet 7 of 17 US 2017/0202238 A1 OSSOVING STEWAN 5% DISSOLVNGA FIRST MEASUREMENT OF GJASOME MIX WW OF WATER ATA (GM1) INCLUDING REDUCED GLUTATHONE, PIPERIC ACiD, PREDETERMINED SODUMASCORBATE, ASCORBIC ACID WITH BLEND THAT EMPERATURE SOAS TO INCLUDES CITRUS BIOFLAVONALS (MIX) AND FORMSUBCOMPOSION- PTEROSI BENE N WAER ATA TEMPERATURE NO MORE THAN 65°C SOASO FORMSUBCOMPOSITION-- G3 ADONG AND/OR MIXNG SUBCOMPOSITON-O SUBCOMPOSION-- SOASO FORM SUBCOMPOSION-- G4 ADONG AND/OR MXING SURFACTANT/EMUSIFIER AND/OR CRIC ACD TO SUBCOMPOSITION-I, SOASO FORMSUBCOMPOSITION-IV G5 SRRING SUBCOMPOSION-IV FOR A PREDETERMINED ME PERIOD SOASO FORM SUBCOMPOSITION-V, i.e., AQUEOUS PHASE (A1) FIG 7 Patent Application Publication Jul. 20, 2017 Sheet 8 of 17 US 2017/0202238 A1 MIXING PHOSPHOLIPID FRACTION, e.g., SUNFLOWER LECITHIN, AND MIXE) iOCOPHEROLS TOGETHER UNDER 65°C SRRING MIX OF SUNFOWER LECHNANOMXEO TOCOPHEROS FOR ABOUT 2 MNUTES SOASO FORM SUBCOMPOSITION-VI, i.e., OIL PHASE (O1) FIG. 8 Patent Application Publication Jul. 20, 2017 Sheet 9 of 17 US 2017/0202238 A1 PREPARNG ACRUEOUS. PHASE {A}) PREPARNG OLPHASE {O1) G8 ADDING O AND A NSAINESS SEE WESSEL PERFORMING FIRS-OMOGENZNG SEP BY MIXNGA AND OA OOO RPM PROGRESSIVELY INCREASING TO 5,000 RPM FOR ABOUT 30 MINUTES US NGA HIGH S.HEAR HOMOGENZER SOAS TO FORMA/O COMPOSION GO ADDING ASECOND MEASUREMENT OF GLUTASOME MIX (GM2) TO A1/O1 COMPOSITION G1 PERFORMING SECONO-OMOGENZING SEP BY MXING AFC COMPOSION AND GM2AT 1,000 RPM PROGRESSIVELY INCREASING TO 5,000 RPM FOR ABOUT OMINUTES A & 65°C SOASO FORMA1/O1/GM2 COMPOSITION G12 ADDING FIRST FLAVOR (F1) INCLUDING OiL PHASE FLAVOR O A1AOfGM2 COMPOSION G 3 PERFORMING-IRD -OMOGENZNG SEP BY MXNG A?OfCSM2 COMPOSITION AND F A 1,000 RPM PROGRESSIVELY INCREASING TO 5,000 FOR ABOU 3-5 MNUTES A & 65°C SOAS TO FORMAFOf GM2/F COMPOSION G4 ADDING GUMARABIC DESPERSED WITH GYCERN (GG) TO AfCFGM2/F COMPOSION G15 PERFORMING FOURH HOMOGENIZING SEP BY WIXING A/C1/GM2/F COWPOSION AND GGA ABOUT 1,000 RPM PROGRESSIVELY INCREASING TO ABOUT 5,000 FOR ABOUT 10 MNUTES A & 65C SOASO FORMAFO (GM2/FlfGG COMPOSION G16 ADDING SECOND FAVOR (F2) INCLUDING ACRUEOUS. PHASE FLAVOR TO AfCAGM2(F1/OGG COMPOSION G17 PERFORMING FIFH HOMOGENIZING STEP OF AFO1/GM2/FFGG COMPOSION AND F2ATABOUT 1,000 RPM PROGRESSIVEY INCREASING TO ABOUT 3500 RPM FOR ABOUT OMNUESA 65C SOASO FORMA1/OfCGM2/F GGF2 COMPOSION G8 MATURING A1/O1/GM2/F1/GGF2 COMPOSITION (DIETARY SUPPLEMENT) FOR6 HORS G19 NIROGEN FLUSHING AfC/GM2/F GG/F2 COMPOSION G FIG 9 SPRAY ORYINGAFOfGM2FFAGGF2 COMPOSION Patent Application Publication Jul.
Load more

Recommended publications
  • Computational Studies Reveal Piperine, the Predominant Oleoresin of Black Pepper (Piper Nigrum) As a Potential Inhibitor of SARS-Cov-2 (COVID-19)
    RESEARCH ARTICLES Computational studies reveal piperine, the predominant oleoresin of black pepper (Piper nigrum) as a potential inhibitor of SARS-CoV-2 (COVID-19) Prassan Choudhary1, Hillol Chakdar1,*, Dikchha Singh1, Chandrabose Selvaraj2, Sanjeev Kumar Singh2, Sunil Kumar3 and Anil Kumar Saxena1 1ICAR-National Bureau of Agriculturally Important Microorganisms, Kushmaur, Mau 275 103, India 2Department of Bioinformatics, Alagappa University, Karaikudi 630 003, India 3Centre for Agricultural Bioinformatics, ICAR-Indian Agricultural Statistics Research Institute, New Delhi 110 002, India receptor binding conformations8. Inhibitors of protease In this study, we screened 26 bioactive compounds have the risk of causing severe side effects as they can in- present in various spices for activity against SARS- 9 CoV-2 using molecular docking. Results showed that hibit the cellular homologous proteases non-specifically . piperine, present in black pepper had a high binding Whole genome sequencing (WGS) has played a crucial affinity (–7.0 kCal/mol) than adenosine monophos- role in paving the way for exploration of novel drug phate (–6.4 kCal/mol) towards the RNA-binding pock- targets10. The GISAID database has undertaken a global et of the nucleocapsid. Molecular dynamics simulation initiative and currently holds WGS of approximately of the docked complexes confirmed the stability of 9300 different isolates of SARS-CoV-2, characterizing piperine docked to nucleocapsid protein as a potential the epidemiology and functional annotation of this virus inhibitor of the RNA-binding site. Therefore, piperine genome (https://www.gisaid.org/). seems to be potential candidate to inhibit the packag- Nucleocapsid (NC) is a highly conserved zinc finger ing of RNA in the nucleocapsid and thereby inhibiting structural protein which plays a crucial role in viral repli- the viral proliferation.
    [Show full text]
  • Steroidal Glycoside Compounds As Core 2 Glcnac-T Inhibitors
    (19) TZZ_ZZ _T (11) EP 1 909 802 B1 (12) EUROPEAN PATENT SPECIFICATION (45) Date of publication and mention (51) Int Cl.: of the grant of the patent: A61K 31/704 (2006.01) A61K 31/7048 (2006.01) 21.05.2014 Bulletin 2014/21 A61K 31/706 (2006.01) A61P 1/04 (2006.01) A61P 1/06 (2006.01) A61P 1/16 (2006.01) (2006.01) (2006.01) (21) Application number: 06755733.0 A61P 3/10 A61P 7/02 A61P 9/10 (2006.01) A61P 11/00 (2006.01) A61P 11/06 (2006.01) A61P 13/12 (2006.01) (22) Date of filing: 06.07.2006 A61P 17/06 (2006.01) A61P 31/18 (2006.01) A61P 19/02 (2006.01) (86) International application number: PCT/GB2006/002518 (87) International publication number: WO 2007/003957 (11.01.2007 Gazette 2007/02) (54) Steroidal glycoside compounds as core 2 GlcNAc-T inhibitors Steroidale Glycosid-Verbindungen als core 2 GlcNAc-T-Hemmer Glycosides steroïdiens en tant qu’inhibiteurs du core 2 GlcNAc-T (84) Designated Contracting States: WO-A-2004/062675 WO-A-2005/060977 AT BE BG CH CY CZ DE DK EE ES FI FR GB GR WO-A-2005/120535 WO-A2-01/32679 HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI WO-A2-02/069980 CN-A- 1 415 625 SK TR US-A- 4 602 003 US-A- 5 104 856 US-A1- 2003 148 962 (30) Priority: 06.07.2005 GB 0513881 • DATABASE WPI 7 January 2004 (2004-01-07), (43) Date of publication of application: Derwent Publications Ltd., London, GB; Class 16.04.2008 Bulletin 2008/16 042,page 3, AN 2004-239758 XP002409228 HUANG H, LIU Z: "Medicine composition for (60) Divisional application: treating myocardial ischemia, angina pectoris 11163561.1 / 2 382 979 and cardiac infarction"
    [Show full text]
  • Print This Article
    PEER-REVIEWED ARTICLE bioresources.com GC-MS Characterisation of Sapogenins from Sisal Waste and a Method to Isolate Pure Hecogenin Jener David G. Santos * and Alexsandro Branco ** Five steroidal sapogenins (tigogenin, neotigogenina, hecogenin, gloriogenin, and dehydrohecogenin) were characterised by gas chromatography coupled with mass spectrometry (GC-MS) from a hydrolysed extract of sisal waste. In addition, pure hecogenin, an important raw material for the pharmaceutical industry, was obtained from this waste by selective liquid-liquid extraction of saponins with only hecogenin as aglycone, followed by acid hydrolysis. The yield of pure hecogenin was 460 mg.Kg-1 of sisal waste. Keywords: Agave sisalana; Sisal waste; Extraction; Steroids; Hecogenin Contact information: Laboratory of Phytochemistry, State University of Feira de Santana, 44.036-900 Feira de Santana, Bahia, Brazil; Corresponding authors: *[email protected], **[email protected] INTRODUCTION Steroidal sapogenins are a glycone non-sugar portion of the saponin molecule used for the semi-synthesis of bioactive compounds. Example compounds used in this application include the following: smilagenin, sarsasapogenin, diosgenin, yamogenin, tigogenin, neotigogenin, gloriogenin, gentrogenin, hecogenin, sisalagenin, 9-dehydro- hecogenin, and gitogenin (Agrawal et al. 1985). Among these steroidal sapogenins, diosgenin, sarsasapogenin, and hecogenin are particularly important. The usefulness of hecogenin (Fig. 1) as a synthetic starting material is due to the presence of an oxygen atom in the C-12 position that can be moved to the C-11 position. This makes it possible to introduce the 9-11 double bond required for the syntheses of corticosteroids (Beauvoir 1976). Fig. 1. Chemical structural of hecogenin In the 1940s, steroidal sapogenins achieved great economic importance because of their transformation into pharmaceutically valuable derivatives such as corticosteroids (prednisone, dexamethasone, betamethasone, triamcinolone, and others), sexual hormones, and steroid diuretics (Fernández-Herrera et al.
    [Show full text]
  • Naturally Occurring Saponins: Chemistry and Biology
    Journal of Poisonous and Medicinal Plant Research Vol. 1(1), pp. 001-006, May, 2013 Available online at http://www.apexjournal.org ISSN 2315-8834© 2013 Apex Journal International Review Naturally occurring saponins: Chemistry and biology J. S. Negi 1*, P. S. Negi 2, G. J. Pant 2, M. S. M. Rawat 2, S. K. Negi 3 1Herbal Research and Development Institute, Mandal, Gopeshwar (Chamoli) - 246 401, Uttarakhand, India. 2Department of Chemistry, HNB Garhwal University, Srinagar (Garhwal)- 246 174, Uttarakhand, India. 3Department of Botany, HNB Garhwal University, Srinagar (Garhwal) - 246 174, Uttarakhand, India. Accepted 2 April, 2013 Naturally occurring saponins are glycosides of steroids, alkaloids and triterpenoids. They are widely distributed in nature and reported to be present in 500 genera of plants. A wide variety of plants belonging to family Liliaceae, Dioscoreaceae, Solanaceae, Sapindaceae and Agavaceae are the major source of saponins. They are amorphous substances having high molecular weight and are soluble in water and alcohol to produce foam but organic solvents inhibit their foaming property. Plants saponins are generally extracted into butanol through liquid-liquid partition and separated through column chromatography using silica gel as adsorbent and chloroform: methanol as mobile phase. HPLC, GC, Sephadex LH-20 Chromatography, DCCC, preparative paper chromatography and TLC were also used for the separation and isolation of saponins. The structures of saponins were determined by several spectroscopic techniques, viz., UV, IR, 1H NMR, 13 C NMR and Mass spectroscopy. Saponins possess several biological activities such as antioxidant, immunostimulant, antihepatotoxic, antibacterial, anticarcinogenic, antidiarrheal, antiulcerogenic, antioxytoxic, antihypoglycemic, anticytotoxic and antimolluscicidal. Saponins are biologically synthesized by C5 isoprene units through cytosolic mevalonate pathway.
    [Show full text]
  • Phytochemical and Biological Investigations of Asparagus Adscendens and Trillium Govanianum
    Phytochemical and Biological Investigations of Asparagus adscendens and Trillium govanianum By Kashif Maqbool Khan CIIT/FA12-R60-004/ATD PhD Thesis In Pharmacy COMSATS University Islamabad, Abbottabad Campus - Pakistan Fall, 2018 COMSATS University Islamabad Phytochemical and Biological Investigations of Asparagus adscendens and Trillium govanianum A Thesis Presented to COMSATS University Islamabad, Abbottabad Campus In partial fulfillment of the requirement for the degree of PhD (Pharmacy) By Kashif Maqbool Khan CIIT/FA12-R60-004/ATD Fall, 2018 ii Phytochemical and Biological Investigations of Asparagus adscendens and Trillium govanianum A Post Graduate Thesis submitted to the Department of Pharmacy as partial fulfillment of the requirement for the award of Degree of Ph.D in Pharmacy. Name Registration Number Kashif Maqbool Khan CIIT/FA12-R60-004/ATD Supervisor Dr. Abdul Manann Associate Professor Department of Pharmacy COMSATS University Islamabad, Abbottabad Campus Co-Supervisor Dr. Muhammad Arfan Associate Professor Department of Chemistry, School of Natural Sciences (SNS) National University of Sciences & Technology (NUST), Islamabad iii iv v vi vii DEDICATION Dedicated to my family and friends who were the pillars of support during my PhD study viii ACKNOWLEDGEMENTS I bow my head before Almighty Allah, The omnipotent, The omnipresent, The merciful, The most gracious, The compassionate, The beneficent, who is the entire and only source of every knowledge and wisdom endowed to mankind and who blessed me with the ability to do this work. It is the blessing of Almighty Allah and His Prophet Hazrat Muhammad (Sallallaho Alaihe Wasallam) which enabled me to achieve this goal. I would like to take this opportunity to convey my cordial gratitude and appreciation to my worthy, reverently and zealot supervisor Dr.
    [Show full text]
  • Yamogenin 3-O-β- <Emphasis Type="Smallcaps">D
    J. Chem. Sci., Vol. 116, No. 2, March 2004, pp. 79–82. © Indian Academy of Sciences. Yamogenin 3–O–b–D-glucopyranosyl (1 ® 4)–O–a–D-xylopyranoside from the seeds of Trigonella foenum-graecum V K SAXENA* and ALBERT SHALEM Department of Chemistry, Dr H S Gour University, Sagar 470 003, India e-mail: [email protected] MS received 17 March 2003; revised 10 June 2003 Abstract. Trigonella foenum-graecum (NO – Leguminosae) is cultivated in many parts of India. It has been found to possess significant medicinal value. Its leaves are used both internally and externally to re- duce swelling, prevent falling of hair and in treating burns. Its seeds are carminative, antipyretic, anthelmintic and diuretic, and are also useful in treatment of dropsy, chronic cough, heart diseases, bron- chitis, piles and small pox. This plant also possesses antidiabetic property. The present communication deals with the isolation and identification of steroidal saponin SA-III, characterised as yamogenin 3–O–b–D-glucopyranosyl (1 ® 4)–O–a–D-xylopyranoside, which was iso- lated from the seeds of Trigonella foenum-graecum. Keywords. Trigonella foenum-graecum; Leguminosae; steroidal saponin; yamogenin 3–O–b–D-gluco- pyranosyl (1 ® 4)–O–a–D-xylopyranoside. 1. Introduction trum of SA-III shows the spiroketal nature of SA- III. It shows all characteristic colour reactions of Trigonella foenum–graecum1–3 (NO – Leguminosae) steroidal saponin9,10 and also gives positive Molisch and is cultivated in many parts of India. It has been test for glycoside. found to possess significant medicinal value. Its Compound SA-III on acid hydrolysis yielded SA- leaves are used both internally and externally to re- III (A), C27H42O3, m.pt.
    [Show full text]
  • Piperine : a Bioenhancer
    International Journal of Pharmacy Research and Technology 2011, Volume 1, Issue 1, 01-05. ISSN xxxx-xxxx Review article Piperine : A Bioenhancer * Amar Singh , Amar Deep *Smt. Tarawati Institute of Bio-Medical & Allied Sciences, Roorkee-Dehradun Road, Saliyar, Roorkee, Dist. Haridwar- 247667, *Corresponding author: E-mail: [email protected] Received: 16/07/2011, Revised: 18/08/2011, Accepted: 12/09/2011 ABSTRACT Piperine is a major alkaloid of Pepper fruits belonging to family Piperaceae which has a number of medicinal proper- ties. Pepper fruits are one of them which have been established as bioenhancer for some selected drugs. In Ayureveda since centuries it is called as “Yogvahi”. Black pepper fruits are the supporting evidence where piperine was one of the ingredients of “Trikatu”. Black pepper fruits are used in different food, drink, dessert, perfume as a brandy flavors and preservative of pickles. It is well known as “King of Spices”. Piperine enhances the bioavailability of structurally and therapeutically differ- ent drugs, either by increasing the absorption or by delaying the metabolism of the drug or by a combination of both processes. It is evident that black pepper fruits have been used as bio-enhancer in allopathic system of medicine. Piperine has been used as bioenhancer for certain antibacterial- antibiotics with promising results. The interaction of piperine with drug- metabolizing enzymes is responsible for oxidation, hydroxylation and glucuronidation. Piperine appears to top in the list of bioenhancers as it has been used as bioenhancer for Allopathic, Ayurvedic and Unani drugs. Piperine enhances Cmax of different drugs significantly. KEY WORDS: Black Pepper, Bio-enhancer, Trikatu, Piper nigrum, Piperine.
    [Show full text]
  • Sample Title
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Indonesian Journal of Cancer Chemoprevention Indonesian Journal of Cancer Chemoprevention, June 2011 ISSN: 2088–0197 e-ISSN: 2355-8989 Activity of Trigonella foenum-graecum on Some Cell Lines Kurnia Agustini*, Wahono Sumaryono, and R. Micho Widyanto Center for Pharmaceuticals and Medical Technology Laboratory for Development of Industrial Agro and Biomedical Technology Agency for the Assessment and Application of Technology (BPPT) Kawasan PUSPIPTEK, Serpong – Indonesia Abstract Trigonella foenum-graecum (TFG) is one of medicinal plants contains some steroidal sapogenin such as diosgenin, yamogenin, gitogenin, tigogenin and trigoneoside, also alkaloid trigonellin, which is have many activity as antidiabetic, estrogenic and also as anti cancer. This experiment was done to explore the activity of some extract of TFG on some cell lines such as MCF7 (Human Breast Cancer Cell-line), T47D (Human Breast Cancer Cell-line), PC3 (Human Prostate Cell-line) and SKOV (Human Ovarian Carcinoma Cell-line). This assay was done using MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium) methods. Results showed that ethyl acetate fraction gives the lowest IC50 than another extracts. IC50 for PC3 is 66.24 ppm, IC50 for MCF7 is 41.81 ppm, IC50 for T47D is 58.63 ppm. These datas can be used for further research to isolate the active compound from TFG. Keywords: Trigonella foenum-graecum, MCF-7, T47D, SKOV, PC3. INTRODUCTION Empirically fenugreek was use as aphrodisiac, carminative, diuretic, emmenagogue, emollient, Fenugreek seed or Foenigraeci semen is galactogogue and tonic. Many researches, dried seed from Trigonella foenum-graecum L., preclinically and clinically, showed that fenugreek Leguminosae, (Anonim, 1979).Geographical have activity for diabetic disease (Annida and distribution of Fenugreek is indigenous to the Stanley 2004).Fenugreek also have estrogenic Mediterranean region, China, India and Indonesia.
    [Show full text]
  • Identification and Characterization of Piperine Synthase from Black
    ARTICLE https://doi.org/10.1038/s42003-021-01967-9 OPEN Identification and characterization of piperine synthase from black pepper, Piper nigrum L. Arianne Schnabel 1, Benedikt Athmer1, Kerstin Manke1, Frank Schumacher2, Fernando Cotinguiba 3 & ✉ Thomas Vogt 1 Black pepper (Piper nigrum L.) is the world’s most popular spice and is also used as an ingredient in traditional medicine. Its pungent perception is due to the interaction of its major compound, piperine (1-piperoyl-piperidine) with the human TRPV-1 or vanilloid receptor. We now identify the hitherto concealed enzymatic formation of piperine from piperoyl coenzyme A and piperidine based on a differential RNA-Seq approach from developing black pepper 1234567890():,; fruits. This enzyme is described as piperine synthase (piperoyl-CoA:piperidine piperoyl transferase) and is a member of the BAHD-type of acyltransferases encoded by a gene that is preferentially expressed in immature fruits. A second BAHD-type enzyme, also highly expressed in immature black pepper fruits, has a rather promiscuous substrate specificity, combining diverse CoA-esters with aliphatic and aromatic amines with similar efficiencies, and was termed piperamide synthase. Recombinant piperine and piperamide synthases are members of a small gene family in black pepper. They can be used to facilitate the microbial production of a broad range of medicinally relevant aliphatic and aromatic piperamides based on a wide array of CoA-donors and amine-derived acceptors, offering widespread applications. 1 Leibniz Institute of Plant Biochemistry, Dept. Cell and Metabolic Biology, Halle (Saale), Germany. 2 Core Facility Vienna Botanical Gardens, Vienna, Austria. ✉ 3 Instituto de Pesquisas de Produtos Naturais (IPPN), Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro/RJ, Brasil.
    [Show full text]
  • ICP TRM 002 E Eng.Pdf (6.079Mb)
    ICP /TFYJ 002-E 10 Oc tober 1986 ENGLl SH ONLY REPORT ~IENTIFIC GROUP ON HERBAL MEDICINE RESEARCH ~ Convened by the REGIONAL OFFICE FOR THE WESTERN PACIFIC OF THE WORLD HEALTH ORGANIZATION Tokyo, Japan, 10-12 March 1986 Not for sale Printed and Distributed by the Regional Office for the Western Pacific of the World Health Organization Manila, Philippines October 1986 'Wh0/ A4aoila, l)jJiLij.);)L.... ..; "OtE The vie~. expressed in thi. repott are those of the participants in the meeting of the Scientific Gtoup ott Herbal Medicine Research and do not necessarily teflect the policit. of the organization. This report has been prep"rteo ty the Regional Ottice for the liestern Pacific of the World Health organization for the governn,ents of ~len.ber States in the Region and for the participants in the meeting of the Scientific Group on Herbal Meaicine Research which was held in Tokyo, Japan, fron, 10 to 12 March 1980. - ii - CONTENTS 1. INTRODUCTION . ,. .. ,. ............................... ,. ...... 1 1.1 Background ... ..•.. .•...... ••. ..•. .... ..•. .••..•••..• 1 1.2 Objectives of the Scientific Group •••••••••••••••••• 1 2. ORGANIZATION OF THE SCIENTIFIC GROUP •••••••••••••••••••• 2 2.1 Participants .......•....•.• ,........................ 2 2.2 Opening address ...................................... 2 2.3 Selection of officers ••••••••••••••••••••••••••.•..• 2 2.4 Agenda •••...••.....•...••.•....•...•..••...•.••..... 2 2.5 Working documents ..•....••••...••.•••.•.•..•••••.••. 2 2.6 Closing session.....................................
    [Show full text]
  • Piperine-Type Amides: Review of the Chemical and Biological Characteristics
    International Journal of Chemistry; Vol. 5, No. 3; 2013 ISSN 1916-9698 E-ISSN 1916-9701 Published by Canadian Center of Science and Education Piperine-Type Amides: Review of the Chemical and Biological Characteristics Simon Koma Okwute1 & Henry Omoregie Egharevba2 1 Department of Chemistry, Faculty of Science, University of Abuja, Gwagwalada, F. C. T., Nigeria 2 National Institute for Pharmaceutical Research and Development (NIPRD), Idu, Industrial Layout, Garki, Abuja, Nigeria Correspondence: Simon Koma Okwute, Department of Chemistry, Faculty of Science, University of Abuja, P.M.B. 117, Gwagwalada, F. C. T., Abuja, Nigeria. Tel: 234-803-595-3929. E-mail: [email protected] Received: June 13, 2013 Accepted: July 20, 2013 Online Published: July 28, 2013 doi:10.5539/ijc.v5n3p99 URL: http://dx.doi.org/10.5539/ijc.v5n3p99 Abstract A new group of alkaloids emerged in 1819 following the isolation of piperine from the fruits of Piper nigrum. Since then, a large number of these compounds now referred to as piperine-type alkaloids or alkamides or piperamides have been isolated commonly from species belonging to the genus piper (piperaceae) which have worldwide geographical distribution. As a result of the traditional uses of piper species as spices in foods and in phytomedicines globally a number of their extractives and indeed the constituent amides have been screened for pharmacological properties. The biogenesis of the amides has been investigated and a number of synthetic pathways have been developed to make them readily available for biological studies. It has now been established that piperine and its analogues are potential pesticides and possess a number of medicinal properties.
    [Show full text]
  • "Saponins," In: Ullmann's Encyclopedia of Industrial Chemistry
    Article No : a23_485 Saponins MICHAEL W. SCHWARZ, E. Merck OHG, Darmstadt, Federal Republic of Germany 1. Introduction......................... 177 3.2. Glycoalkaloids ...................... 183 1.1. General Properties.................... 178 3.3. Triterpene Saponins................... 184 1.2. Distribution ......................... 178 4. Animal Saponins ..................... 188 1.3. Isolation ............................ 178 4.1. Asterosaponins....................... 188 1.4. Structure Elucidation.................. 179 4.2. Holothurins ......................... 189 1.5. Analysis ............................ 179 References .......................... 189 2. Pharmacology ....................... 179 3. Plant Saponins ....................... 180 3.1. Steroid Saponins ..................... 180 Abbreviations galA: b-D-galacturonic acid glc: b-D-glucose api: b-D-apiose glcA: b-D-glucuronic acid ara: a-L-arabinose 0 qui: b-D-quinovose drib: b-D-2 -deoxyribose rha: a- -rhamnose f: furanoside L xyl: b-D-xylose fuc: b-D-fucose fru: b-D-fructose gal: b-D-galactose 1. Introduction Saponins are categorized according to the structure of the aglycone moiety (sapogenin) and Saponins are glycosides (see ! Carbohydrates: the number of linked sugar chains. Aglycones Occurrence, Structures and Chemistry) occur- can be divided into triterpenoid and steroid ring primarily in plants but also in starfish sapogenins. Steroid saponins with basic proper- (Asteroidea) and sea cucumbers (Holothuridea). ties are termed glycoalkaloids. Properties generally considered to be shared by Hexoses common in all saponins are b-D- this group of natural products are surfactant glucose (glc), b-D-galactose (gal), and a-L- activity, hemolytic action, steroid- complexing rhamnose (rha); pentoses are a-L-arabinose ability, and biocidal capability. (ara) and b-D-xylose (xyl). Pentoses occur The characteristic soapy lather formed when primarily as pyranosides, less frequently as saponin- containing plant extracts are agitated in furanosides (f).
    [Show full text]