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Direct Analysis of Hops by Leaf Spray and Paper Spray Mass Spectrometry
Western Michigan University ScholarWorks at WMU Master's Theses Graduate College 12-2012 Direct Analysis of Hops by Leaf Spray and Paper Spray Mass Spectrometry Kari Blain Follow this and additional works at: https://scholarworks.wmich.edu/masters_theses Part of the Chemistry Commons Recommended Citation Blain, Kari, "Direct Analysis of Hops by Leaf Spray and Paper Spray Mass Spectrometry" (2012). Master's Theses. 100. https://scholarworks.wmich.edu/masters_theses/100 This Masters Thesis-Open Access is brought to you for free and open access by the Graduate College at ScholarWorks at WMU. It has been accepted for inclusion in Master's Theses by an authorized administrator of ScholarWorks at WMU. For more information, please contact [email protected]. DIRECT ANALYSIS OF HOPS BY LEAF SPRAY AND PAPER SPRAY MASS SPECTROMETRY Kari Blain, M.S. Western Michigan University, 2012 The objective of this research is to develop a new and innovative method of hops analysis, which is much faster than standard testing methods, as well as reduce the amount of consumables and solvent used. A detailed discussion on the development of an ambient ionization mass spectrometry method called paper spray (PS-MS) and leaf spray (LS-MS) mass spectrometry will be presented. This research investigates the use of PS-MS and LS-MS techniques to determine the α- and β- acids present in hops. PS-MS and LS-MS provide a fast way to analyze hops samples by delivering data as rapidly as a UV-Vis measurement while providing information similar to lengthy liquid chromatographic separations. The preliminary results shown here indicate that PS-MS could be used to determine cohumulone and α/β ratios. -
Analytical Standards for Human Exposure Analysis
ENVIRONMENTAL STANDARDS Cambridge Isotope Laboratories, Inc. isotope.com Analytical Standards for Human Exposure Analysis Human Personal Care Industrial Products Pollution Dermal Inhalation Ingestion Food Tobacco Products Drinking Water Pharmaceuticals Cambridge Isotope Laboratories, Inc. North America: 1.800.322.1174 [email protected] | International: +1.978.749.8000 [email protected] | fax: 1.978.749.2768 | isotope.com Cambridge Isotope Laboratories, Inc. | Analytical Standards for Human Exposure Analysis Analytical Standards for Human Exposure Analysis Exposomics is the study of the exposome and is a field that has been gaining attention as researchers focus not only on identifying contaminants present in the environment, but their effects on humans. The exposome encompasses an individual’s lifetime exposure to internal and external stresses, including environmental, dietary, and metabolic factors, to name a few. Cambridge Isotope Laboratories, Inc. (CIL) has and continues to produce standards that are used in many leading biomonitoring research projects, such as the US Centers for Disease Control and Prevention (CDC) “National Health and Nutrition Examination Survey (NHANES)” and the Japan National Institute for Environmental Studies (NIES) “Japan Environment and Children’s Study (JECS).” The CDC’s NHANES program is focused on assessing the health and nutritional status of both children and adults in the United States. There are several studies involved in this program, which is notable in that both interviews and physical examinations are factored in when compiling data. The first NHANES study was conducted from 1971-1975 and subsequent studies are continuing to this day. Additional information about the NHANES program can be found at www.cdc.gov/nchs/nhanes/index.htm. -
ACE Food Beverage Applications Guide
Food and Beverage Applications Guide Ultra-Inert Base Deactivated UHPLC / HPLC Columns Contents Application Index 1 Analyte Index 2 - 4 Application Notes 5 - 90 Send us your application and receive a free ACE column Send us your application on an ACE column and help extend our applications database. Your proven method will enable your chromatography colleagues to benefit and if we select your application for publication we’ll send you a FREE ACE analytical column of your choice. To submit your application e-mail us at: [email protected] ACE ® Food and Beverage Applications: Application Index Application Pages Application Pages Additives and intense sweeteners 5 Organic acids 2 47 Argicultural pesticides 6 Organic acids 3 48 Amino acids in peas 7 Organic acids 4 49 Amino acids and biogenic amines in wine and beer 8 Organophosphorus flame retardants in water by LC-MS/MS 50 Aminoglycosides in eggs 9 Organophosphorus isomer flame retardants in water 51 Annatto 10 Paraben preservatives 52 Anthocyanins from sambucus nigra (elderberry) 11 Perfluoro acids by LC-MS/MS 53 Appetite suppressants by LC-MS 12 Perfluoroalkyl substances by ion pairing LC-MS/MS 54 Arsenolipids from edible seaweed 13 Perfluorinated compounds in water by LC-MS/MS 55 Artificial colours (water soluble) 14 Pesticides (250 analytes) by LC-MS/MS 56 Artificial food colouring 15 Pesticides (47 analytes) by LC-MS/MS 60 Artificial sweeteners global method 16 Pesticides in water 61 Artificial sweeteners (stevia glycosides) 17 Phenolic compounds in ground water and landfill leachates -
Masterarbeit / Master's Thesis
MASTERARBEIT / MASTER’S THESIS Titel der Masterarbeit / Title of the Master‘s Thesis Optimization of an LC-MS/MS Method for the Determination of Xenobiotics in Biological Matrices verfasst von / submitted by Thomas Jamnik BSc angestrebter akademischer Grad / in partial fulfilment of the requirements for the degree of Master of Science (MSc) Wien, 2020 / Vienna 2020 Studienkennzahl lt. Studienblatt / UA 066 863 degree programme code as it appears on the student record sheet: Studienrichtung lt. Studienblatt / Masterstudium Biologische Chemie degree programme as it appears on the student record sheet: Betreut von / Supervisor: Assoz. Prof. Dipl.-Ing. Dr. Benedikt Warth, Bakk.techn. 1 2 Erklärung Ich erkläre, dass die vorliegende Masterarbeit von mit selbst verfasst wurde und ich keine anderen als die angeführten Behelfe verwendet bzw. mich auch sonst keiner unerlaubter Hilfe bedient habe. Ich versichere, dass diese Arbeit bisher weder im In- noch Ausland in irgendeiner Form als Prüfungsarbeit vorgelegt wurde. Ich habe mich bemüht, sämtliche Inhaber der Bildrechte ausfindig zu machen und ihre Zustimmung zur Verwendung der Bilder in dieser Arbeit eingeholt. Sollte dennoch eine Urheberrechtsverletzung bekannt werden, ersuche ich um Meldung bei mir. Danksagung Ich danke Dr. Benedikt Warth nicht nur für die Möglichkeit diese interessante Masterarbeit verfassen zu dürfen, sondern auch für die gewonnenen Erfahrungen die der Einblick in seine Arbeitsgruppe und das Institut für Lebensmittelchemie erlaubt hat. Besonderer Dank gilt meiner direkten Betreuerin Dipl.-Ing. Mira Flasch, welche stets hilfsbereite Unterweisung in die Praxis als auch Theorie der verwendeten Arbeitsmethoden gab, immer für ausgiebige Diskussionen bereit stand und sich viel Zeit für diverse Korrekturen dieser Arbeit nahm. -
Environmental Chemical Test Results Prepared For: Anjuli S
Environmental Chemical Test Results Prepared for: Anjuli S. th Sample collection date: October 5 , 2020 Pg 1 of 23 Environmental Chemical Test Results Prepared for: Sample Collection Date: th Anjuli S. October 5 , 2020 Below are the results for your exposures to everyday toxic chemicals and phytoestrogens. This includes your personalized recommendations based on your lifestyle audit and exposures. This information is for educational purposes only and is not intended to diagnose or treat any health conditions. Contact us for questions or feedback, or see the FAQs for answers to general questions about your test. Report Table of Contents BISPHENOLS 2 PARABENS 5 PHTHALATES 10 OTHER EVERYDAYSample TOXIC CHEMICALS 14 MYCOTOXINS 19 PHYTOESTROGENS 21 Environmental Chemical Test Results Prepared for: Anjuli S. th Sample collection date: October 5 , 2020 Pg 2 of 23 BISPHENOLS Bisphenol A Test Results About Click here for information about sources of exposure and health effects. Test Results Total Bisphenol A (BPA) = Not Detected* Your level was not-detected, indicating LOW levels of exposure. Report Sample Environmental Chemical Test Results Prepared for: Anjuli S. th Sample collection date: October 5 , 2020 Pg 3 of 23 Bisphenol S Test Results About Click here for information about sources of exposure and health effects. Test Results Total Bisphenol S (BPS) = Not Detected* Your level was not-detected, indicating LOW levels of exposure. Report Sample **SAMPLE RECOMMENDATIONS--Your report will be more detailed and tailored to you.** Take Action: Bisphenols Great job! You have low exposures to BPA and BPS. To keep these exposures low: 1. Avoid “Proposition 65” products. -
Priority Pollutant, Endocrine Disruptor, and Chemical Contaminant Standards Solutions for a Greener World Introduction
Priority Pollutant, Endocrine Disruptor, and Chemical Contaminant Standards Solutions for a Greener World Introduction Priority Pollutant, Endocrine Disruptor, and Chemical Contaminant Standards isotope.com Pharmaceutical and Personal Care Product Halogenated and Substituted Benzene Standards and Phenol Standards Concern about environmental and human exposure to Many industrial and consumer products are composed of pharmaceuticals and personal care products (PPCPs) has grown chemicals that contain halogenated or substituted benzene significantly. This classification encompasses a broad range of or phenol functional groups. Resistant to decomposition and chemicals, ranging from antibiotics to hormones to pesticides. metabolism, these chemicals may persist even after the parent One common theme among these groups is the need for molecule has undergone partial decomposition, or they may high-quality isotopically labeled standards to strengthen the exist as a product or an industrial byproduct. The increased analysis of PPCPs in difficult matrices such as sewage sludge use of brominated compounds is expected to lead to more and wastewater. CIL, with guidance from leading laboratories brominated benzenes and phenols in the environment, and around the world, works diligently to produce representative the continued presence of chlorinated compounds ensures standards for the analysis of PPCPs. that chlorinated benzenes and phenols will be found in the environment for years to come. Food and Drinking Water Analysis Standards Bisphenol Standards Increased attention to possible contamination of food and Bisphenol A (BPA) is a synthetic compound that has long been water has caused analysts to broaden the scope of trace food used in the production of polycarbonate plastics and epoxy and water testing by IDMS. -
Determination of Isoxanthohumol, Xanthohumol, Alpha and Beta Bitter
Determination of Isoxanthohumol, Xanthohumol, Alpha and Beta Bitter Acids, and trans and cis-Iso-Alpha Acids by HPLC with UV and Electrochemical Detection: Application to Hop and Beer Analysis Paul A. Ullucci, Ian N. Acworth, and David Thomas Thermo Fisher Scientific, Chelmsford, MA, USA Data Analysis Polyphenol Method – Targeted Analysis FIGURE 2. Principal Component Plots for A) ECD and B) UV Data. TABLE 2. Hops Bitter Acids Data Presented in mg/L. Overview 1 Data were analyzed using Thermo Fisher Dionex Chromeleon Chromatography Data The analytical figures of merit for this assay were described preciously. Briefly, the Beer 1 Beer 2 Beer 3 Beer 4 Purpose: To develop gradient HPLC methods using a spectro-electro array platform for use System 6.8 (SR 9) and CoulArray™ software 3.1. EC-array data were transferred to limits of detection were typically 10−50 pg on column by ECD and 100−500 pg by UV. Beer 7 Compound Ultra IPA Ultra IPA Regular Light Beer to either measure specific analytes in beer samples or in a metabolomic approach to Pirouette® software for chemometric analysis using a CoulArray version 2.0 Software The limits of quantification were 200−1000 pg on column by ECD and 500−5000 pg by A distinguish between different beer samples, as well as study beer stability. Utility (Pattern Recognition Setup Wizard). UV data were tabularized prior to transfer to UV. Response range was over seven orders of magnitude by ECD and five by UV. Factor2 Ultra Isoxanthohumol 2.10 1.3 0.38 0.28 2 Methods: Gradient HPLC with diode-array detection and electrochemical array detection Pirouette. -
Simultánní Stanovení Prenylflavonoidů a Isoflavonoidů Ve Chmelu a Pivu
KVASNY PRUM. Simultánní stanovení prenylflavonoidů a isoflavonoidů ve chmelu a pivu ... 59 / 2013 (2) 41 Simultánní stanovení prenylflavonoidů a isoflavonoidů ve chmelu a pivu metodou HPLC-DAD: Studie aplikace homogenátu zeleného chmele v pivovarském procesu Simultaneous Determination of Prenylflavonoids and Isoflavonoids in Hops and Beer by HPLC-DAD Method: Study of Green Hops Homogenate Application in the Brewing Process MARIE JURKOVÁ 1, Pavel ČEJKA 1, MILAN HOUŠKA 2, ALExANDR MIKYŠKA 1 1 Výzkumný ústav pivovarský a sladařský, a.s., Pivovarský ústav Praha, Lípová 15,120 44 Praha 2 / Research Institute of Brewing and Malting PLC, Brewing Institute Prague, Lípová 15, CZ – 120 44 Prague 2, Czech Republic 2 Výzkumný ústav potravinářský Praha / Food Research Institute Prague – Rádiová 7, CZ – 102 31, Prague 10, Czech Republic e-mail: [email protected] Jurková, M. – Čejka, P. – Houška, M. – Mikyška, A.: Simultánní stanovení prenylflavonoidů a isoflavonoidů ve chmelu a pivu metodou HPLC-DAD: Studie aplikace homogenátu zeleného chmele v pivovarském procesu. Kvasny Prum . 59, 2013, č . 2, s . 41–49 . Polyfenolové látky náležející do skupin prenylflavonoidů a isoflavonoidů mají účinky podporující zdraví a ochranu proti řadě civilizač- ních chorob . Působí jako antioxidanty, mají protirakovinné, antimikrobiální, protizánětlivé vlastnosti, některé jsou fytoestrogeny a půso- bí proti osteoporóze . Byla vypracována nová analytická metoda HPLC-DAD pro simultánní stanovení prenylflavonoidů (xanthohumol, isoxanthohumol, 8-prenylnaringenin, 6-prenylnaringenin) a isoflavonoidů (daidzein, genistein, formononetin a Biochanin A) ve chmelu a pivu a byla provedena studie dopadu chmelení homogenátem zeleného chmele stabilizovaného vysokým tlakem na obsah těchto látek v pivu . Vypracovaná metoda může najít uplatnění při monitorování obsahu bioaktivních flavonoidů ve chmelových surovinách a pivu . -
Recommendation on Perfluorinated Compound Treatment Options for Drinking Water
Recommendation on Perfluorinated Compound Treatment Options for Drinking Water New Jersey Drinking Water Quality Institute Treatment Subcommittee June 2015 Laura Cummings, P.E., Chair Anthony Matarazzo Norman Nelson, P.E. Fred Sickels Carol T. Storms Background At the request of the Commissioner of the New Jersey Department of Environmental Protection the Drinking Water Quality Institute (DWQI) is working to develop recommended Maximum Contaminant Levels (MCL) for three long-chain perfluorinated compounds (PFC): Perfluorononanoic acid (PFNA), Perfluorooctanoic acid (PFOA) and Perfluorooctanesulfonic acid (PFOS). The Treatment Subcommittee of the Drinking Water Quality Institute is responsible for identifying available treatment technologies or methods for removal of hazardous contaminants from drinking water. The subcommittee has met several times over the last year beginning in July 2014 to discuss and investigate best available treatment options for the long-chain (8 – 9 carbon) PFCs identified above. The subcommittee decided to research and report on treatment options for all three compounds, as the treatment options are not expected to differ from compound to compound due to their similar properties (e.g. persistence, water solubility, similar structure, strong carbon-fluorine bonds, and high polarity). This approach contrasts with the other two subcommittees which will address the three compounds separately. The subcommittee has gathered and reviewed data from several sources in order to identify widely-accepted and well-performing strategies for removal of long-chain PFCs, including use of alternate sources. This report is intended to present the subcommittee’s findings. At this time, there are no Federal drinking water standards for PFNA, PFOA or PFOS; however in 2009 the United State Environmental Protection Agency (USEPA, 2009) established a Provisional Health Advisory (PHA) level of 0.4 µg/L for PFOA and 0.2 µg/L PHA for PFOS for short-term exposure. -
Highly Isoxanthohumol Enriched Hop Extract Obtained by Pressurized Hot
ACCEPTED MANUSCRIPT HIGHLY ISOXANTHOHUMOL ENRICHED HOP EXTRACT OBTAINED BY PRESSURIZED HOT WATER EXTRACTION (PHWE). CHEMICAL AND FUNCTIONAL CHARACTERIZATION. Alicia Gil-Ramíreza; José Antonio Mendiolab; Elena Arranza; Alejandro Ruíz- Rodrígueza; Guillermo Regleroa; Elena Ibáñez; Francisco R Marína* a Department of Characterization and production of New Foods. b Department of Bioactivity and Food Analysis. Institute of Food Science Research (CIAL), (Spanish National Research Council–Universidad Autónoma de Madrid), C/Nicolás Cabrera, 9. Campus de la Universidad Autónoma de Madrid, 28049 Madrid, Spain. * Corresponding author: [email protected] +34910017921 ACCEPTED MANUSCRIPT 1 ACCEPTED MANUSCRIPT ABSTRACT Hop (Humulus lupulus) is one of the richest natural sources of a prenylfalvonoids such as xanthohumol (XN), desmetylxanthohumol (DMX), isoxanthohumol (IX) or 8- prenylnaringenin (8-PN), being XN the most abundant of them in the raw material. So far, obtention of prenylflavonoids have been done by chemical synthesis or extraction with organic solvents, with no described methods for the isolation of IX, which has been reported to have anti-inflammatory properties. In this study, pressurized hot water extraction (PHWE) it is shown not only as effective method to extract some prenylflavonoids but to selectively change the relative amount of them, favoring the extraction of IX against XN. Thus, pressurized water extraction at 150ºC showed a high selectivity towards IX, being proposed as a method to enrich natural hop´s extracts in IX. On the other hand, the extracts thus obtained were chemically characterized and evaluated for their anti-inflammatory activity, which was higher than the expected by its content in IX. KEYWORDS: Prenylflavonoids, xanthohumol (XN), isoxanthohumol (IX), Pressurized HotACCEPTED Water Extraction (PHWE), MANUSCRIPT anti-inflamatory. -
UNITED NATIONS Stockholm Convention on Persistent Organic Pollutants Technical Paper on the Identification and Assessment Of
UNITED NATIONS SC UNEP/POPS/POPRC.8/INF/17 Distr.: General 15 August 2012 English only Stockholm Convention on Persistent Organic Pollutants Persistent Organic Pollutants Review Committee Eighth meeting Geneva, 15–19 October 2012 Item 5 (g) of the provisional agenda* Technical work: assessment of alternatives to perfluorooctane sulfonic acid in open applications Technical paper on the identification and assessment of alternatives to the use of perfluorooctane sulfonic acid in open applications Note by the Secretariat 1. In paragraph 6 of decision SC-5/5, the Conference of the Parties to the Stockholm Convention requested the Secretariat, subject to the availability of resources, to commission a technical paper on the identification and assessment of alternatives to the use of perfluorooctane sulfonic acid in open applications for consideration by the Persistent Organic Pollutants Review Committee at its eighth meeting. 2. That technical paper has been prepared, with financial support provided by the Government of Norway, on the basis of the terms of reference and an outline developed by the Committee at its seventh meeting. It is set out in the annex to the present note, where it is presented without formal editing. * UNEP/POPS/POPRC.8/1. K1282361 110912 UNEP/POPS/POPRC.8/INF/17 Annex Technical paper on the identification and assessment of alternatives to the use of perfluorooctane sulfonic acid in open applications 2 UNEP/POPS/POPRC.8/INF/17 Table of contents Executive summary......................................................................................................................................................... -
By Intestinal Microbiota; Conversion of Isoxanthohumol Into 8-Prenylnaringenin
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Ghent University Academic Bibliography J. Agric. Food Chem. 2005, 53, 6281−6288 6281 Activation of Proestrogens from Hops (Humulus lupulus L.) by Intestinal Microbiota; Conversion of Isoxanthohumol into 8-Prenylnaringenin SAM POSSEMIERS,† ARNE HEYERICK,‡ VEERLE ROBBENS,† DENIS DE KEUKELEIRE,‡ AND WILLY VERSTRAETE*,† Laboratory of Microbial Ecology and Technology (LabMET), Faculty of Bioscience Engineering, Gent UniversitysUGent, Coupure Links 653, and Laboratory of Pharmacognosy and Phytochemistry, Faculty of Pharmaceutical Sciences, Gent UniversitysUGent, Harelbekestraat 72, B-9000, Gent, Belgium Hop, an essential ingredient in most beers, contains a number of prenylflavonoids, among which 8-prenylnaringenin (8-PN) would be the most potent phytoestrogen currently known. Although a number of health effects are attributed to these compounds, only a few reports are available about the bioavailability of prenylflavonoids and the transformation potency of the intestinal microbial community. To test these transformations, four fecal samples were incubated with xanthohumol, isoxanthohumol (IX), and 8-PN. Upon incubation with IX, present in strong ales up to 4 mg/L, 36% was converted into 8-PN in one fecal sample and the estrogenic properties of the sample drastically increased. In an experiment with 12 fecal cultures, this conversion was observed in one-third of the samples, indicating the importance of interindividual variability in the intestinal microbial community. Eubacterium limosum was identified to be capable of this conversion (O-demethylation) of IX into 8-PN, and after strain selection, a conversion efficiency of 90% was achieved. Finally, strain supplementation to a nonconverting fecal sample led to rapid and high 8-PN production at only 1% (v/v) addition.