Masterarbeit / Master's Thesis
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Direct Analysis of Hops by Leaf Spray and Paper Spray Mass Spectrometry
Western Michigan University ScholarWorks at WMU Master's Theses Graduate College 12-2012 Direct Analysis of Hops by Leaf Spray and Paper Spray Mass Spectrometry Kari Blain Follow this and additional works at: https://scholarworks.wmich.edu/masters_theses Part of the Chemistry Commons Recommended Citation Blain, Kari, "Direct Analysis of Hops by Leaf Spray and Paper Spray Mass Spectrometry" (2012). Master's Theses. 100. https://scholarworks.wmich.edu/masters_theses/100 This Masters Thesis-Open Access is brought to you for free and open access by the Graduate College at ScholarWorks at WMU. It has been accepted for inclusion in Master's Theses by an authorized administrator of ScholarWorks at WMU. For more information, please contact [email protected]. DIRECT ANALYSIS OF HOPS BY LEAF SPRAY AND PAPER SPRAY MASS SPECTROMETRY Kari Blain, M.S. Western Michigan University, 2012 The objective of this research is to develop a new and innovative method of hops analysis, which is much faster than standard testing methods, as well as reduce the amount of consumables and solvent used. A detailed discussion on the development of an ambient ionization mass spectrometry method called paper spray (PS-MS) and leaf spray (LS-MS) mass spectrometry will be presented. This research investigates the use of PS-MS and LS-MS techniques to determine the α- and β- acids present in hops. PS-MS and LS-MS provide a fast way to analyze hops samples by delivering data as rapidly as a UV-Vis measurement while providing information similar to lengthy liquid chromatographic separations. The preliminary results shown here indicate that PS-MS could be used to determine cohumulone and α/β ratios. -
I LITERATURE-BASED DISCOVERY of KNOWN and POTENTIAL NEW
LITERATURE-BASED DISCOVERY OF KNOWN AND POTENTIAL NEW MECHANISMS FOR RELATING THE STATUS OF CHOLESTEROL TO THE PROGRESSION OF BREAST CANCER BY YU WANG THESIS Submitted in partial fulfillment of the requirements for the degree of Master of Science in Bioinformatics with a concentration in Library and Information Science in the Graduate College of the University of Illinois at Urbana-Champaign, 2019 Urbana, Illinois Adviser: Professor Vetle I. Torvik Professor Erik Russell Nelson i ABSTRACT Breast cancer has been studied for a long period of time and from a variety of perspectives in order to understand its pathogeny. The pathogeny of breast cancer can be classified into two groups: hereditary and spontaneous. Although cancer in general is considered a genetic disease, spontaneous factors are responsible for most of the pathogeny of breast cancer. In other words, breast cancer is more likely to be caused and deteriorated by the dysfunction of a physical molecule than be caused by germline mutation directly. Interestingly, cholesterol, as one of those molecules, has been discovered to correlate with breast cancer risk. However, the mechanisms of how cholesterol helps breast cancer progression are not thoroughly understood. As a result, this study aims to study known and discover potential new mechanisms regarding to the correlation of cholesterol and breast cancer progression using literature review and literature-based discovery. The known mechanisms are further classified into four groups: cholesterol membrane content, transport of cholesterol, cholesterol metabolites, and other. The potential mechanisms, which are intended to provide potential new treatments, have been identified and checked for feasibility by an expert. -
ACE Food Beverage Applications Guide
Food and Beverage Applications Guide Ultra-Inert Base Deactivated UHPLC / HPLC Columns Contents Application Index 1 Analyte Index 2 - 4 Application Notes 5 - 90 Send us your application and receive a free ACE column Send us your application on an ACE column and help extend our applications database. Your proven method will enable your chromatography colleagues to benefit and if we select your application for publication we’ll send you a FREE ACE analytical column of your choice. To submit your application e-mail us at: [email protected] ACE ® Food and Beverage Applications: Application Index Application Pages Application Pages Additives and intense sweeteners 5 Organic acids 2 47 Argicultural pesticides 6 Organic acids 3 48 Amino acids in peas 7 Organic acids 4 49 Amino acids and biogenic amines in wine and beer 8 Organophosphorus flame retardants in water by LC-MS/MS 50 Aminoglycosides in eggs 9 Organophosphorus isomer flame retardants in water 51 Annatto 10 Paraben preservatives 52 Anthocyanins from sambucus nigra (elderberry) 11 Perfluoro acids by LC-MS/MS 53 Appetite suppressants by LC-MS 12 Perfluoroalkyl substances by ion pairing LC-MS/MS 54 Arsenolipids from edible seaweed 13 Perfluorinated compounds in water by LC-MS/MS 55 Artificial colours (water soluble) 14 Pesticides (250 analytes) by LC-MS/MS 56 Artificial food colouring 15 Pesticides (47 analytes) by LC-MS/MS 60 Artificial sweeteners global method 16 Pesticides in water 61 Artificial sweeteners (stevia glycosides) 17 Phenolic compounds in ground water and landfill leachates -
Environmental Chemical Test Results Prepared For: Anjuli S
Environmental Chemical Test Results Prepared for: Anjuli S. th Sample collection date: October 5 , 2020 Pg 1 of 23 Environmental Chemical Test Results Prepared for: Sample Collection Date: th Anjuli S. October 5 , 2020 Below are the results for your exposures to everyday toxic chemicals and phytoestrogens. This includes your personalized recommendations based on your lifestyle audit and exposures. This information is for educational purposes only and is not intended to diagnose or treat any health conditions. Contact us for questions or feedback, or see the FAQs for answers to general questions about your test. Report Table of Contents BISPHENOLS 2 PARABENS 5 PHTHALATES 10 OTHER EVERYDAYSample TOXIC CHEMICALS 14 MYCOTOXINS 19 PHYTOESTROGENS 21 Environmental Chemical Test Results Prepared for: Anjuli S. th Sample collection date: October 5 , 2020 Pg 2 of 23 BISPHENOLS Bisphenol A Test Results About Click here for information about sources of exposure and health effects. Test Results Total Bisphenol A (BPA) = Not Detected* Your level was not-detected, indicating LOW levels of exposure. Report Sample Environmental Chemical Test Results Prepared for: Anjuli S. th Sample collection date: October 5 , 2020 Pg 3 of 23 Bisphenol S Test Results About Click here for information about sources of exposure and health effects. Test Results Total Bisphenol S (BPS) = Not Detected* Your level was not-detected, indicating LOW levels of exposure. Report Sample **SAMPLE RECOMMENDATIONS--Your report will be more detailed and tailored to you.** Take Action: Bisphenols Great job! You have low exposures to BPA and BPS. To keep these exposures low: 1. Avoid “Proposition 65” products. -
Exposure to Endocrine Disruptors During Adulthood: Consequences for Female Fertility
233 3 S RATTAN and others Endocrine disruptors and 233:3 R109–R129 Review female fertility Exposure to endocrine disruptors during adulthood: consequences for female fertility Saniya Rattan, Changqing Zhou, Catheryne Chiang, Sharada Mahalingam, Correspondence should be addressed Emily Brehm and Jodi A Flaws to J A Flaws Department of Comparative Biosciences, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA Email [email protected] Abstract Endocrine disrupting chemicals are ubiquitous chemicals that exhibit endocrine Key Words disrupting properties in both humans and animals. Female reproduction is an important f endocrine disrupting process, which is regulated by hormones and is susceptible to the effects of exposure chemicals to endocrine disrupting chemicals. Disruptions in female reproductive functions f adult by endocrine disrupting chemicals may result in subfertility, infertility, improper f female hormone production, estrous and menstrual cycle abnormalities, anovulation, and f fertility early reproductive senescence. This review summarizes the effects of a variety of Endocrinology synthetic endocrine disrupting chemicals on fertility during adult life. The chemicals of covered in this review are pesticides (organochlorines, organophosphates, carbamates, pyrethroids, and triazines), heavy metals (arsenic, lead, and mercury), diethylstilbesterol, Journal plasticizer alternatives (di-(2-ethylhexyl) phthalate and bisphenol A alternatives), 2,3,7,8-tetrachlorodibenzo-p-dioxin, nonylphenol, polychlorinated biphenyls, triclosan, and parabens. This review focuses on the hypothalamus, pituitary, ovary, and uterus because together they regulate normal female fertility and the onset of reproductive senescence. The literature shows that several endocrine disrupting chemicals have endocrine disrupting abilities in females during adult life, causing fertility abnormalities Journal of Endocrinology in both humans and animals. -
Determination of Isoxanthohumol, Xanthohumol, Alpha and Beta Bitter
Determination of Isoxanthohumol, Xanthohumol, Alpha and Beta Bitter Acids, and trans and cis-Iso-Alpha Acids by HPLC with UV and Electrochemical Detection: Application to Hop and Beer Analysis Paul A. Ullucci, Ian N. Acworth, and David Thomas Thermo Fisher Scientific, Chelmsford, MA, USA Data Analysis Polyphenol Method – Targeted Analysis FIGURE 2. Principal Component Plots for A) ECD and B) UV Data. TABLE 2. Hops Bitter Acids Data Presented in mg/L. Overview 1 Data were analyzed using Thermo Fisher Dionex Chromeleon Chromatography Data The analytical figures of merit for this assay were described preciously. Briefly, the Beer 1 Beer 2 Beer 3 Beer 4 Purpose: To develop gradient HPLC methods using a spectro-electro array platform for use System 6.8 (SR 9) and CoulArray™ software 3.1. EC-array data were transferred to limits of detection were typically 10−50 pg on column by ECD and 100−500 pg by UV. Beer 7 Compound Ultra IPA Ultra IPA Regular Light Beer to either measure specific analytes in beer samples or in a metabolomic approach to Pirouette® software for chemometric analysis using a CoulArray version 2.0 Software The limits of quantification were 200−1000 pg on column by ECD and 500−5000 pg by A distinguish between different beer samples, as well as study beer stability. Utility (Pattern Recognition Setup Wizard). UV data were tabularized prior to transfer to UV. Response range was over seven orders of magnitude by ECD and five by UV. Factor2 Ultra Isoxanthohumol 2.10 1.3 0.38 0.28 2 Methods: Gradient HPLC with diode-array detection and electrochemical array detection Pirouette. -
Czech Republic) and Impacts on Quality of Treated Drinking Water
water Article Pharmaceuticals Load in the Svihov Water Reservoir (Czech Republic) and Impacts on Quality of Treated Drinking Water Josef V. Datel * and Anna Hrabankova T.G. Masaryk Water Research Institute, 16000 Prague, Czech Republic; [email protected] * Correspondence: [email protected]; Tel.: +420-220-197-291 Received: 17 April 2020; Accepted: 6 May 2020; Published: 13 May 2020 Abstract: An important component of micropollutants are PPCPs (pharmaceuticals and personal care products). This paper contains the results of the monitoring of surface water, groundwater and wastewater in the surrounding area of the Svihov drinking water reservoir. Over the period 2017–2019, over 21,000 water samples were taken and analyzed for 112 pharmaceuticals, their metabolites, and other chemicals. The results are discussed in detail for two streams with the highest observed concentration of PPCPs (Hnevkovice, Dolni Kralovice) and two streams with the highest water inflow into the reservoir, representing also the highest mass flow of PPCPs into the reservoir (Miletin, Kacerov). The overall analysis of the results shows that acesulfame, azithromycin, caffeine, gabapentin, hydrochlorothiazide, ibuprofen and its metabolites, oxypurinol, paraxanthine, and saccharin (on some profiles up to tens of thousands ng/dm3) attain the highest concentration and occur most frequently. The evaluation of raw water and treated drinking water quality showed the significant positive effect of water retention in the reservoir (retention time of 413 days) and also of the treatment process, so that the treated drinking water is of high quality and contains only negligible residues of few PPCPs near the detection limit of the analytical method used. -
Endocrine Disruptors
Endocrine disruptors Afke Groen & Christine Neuhold The RECIPES project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 824665 Authors Afke Groen, Maastricht University* Christine Neuhold, Maastricht University * currently works at the think tank Mr. Hans van Mierlo Stichting With thanks to our two anonymous interviewees Manuscript completed in April 2020 Document title WP2 Case study: Endocrine disruptors Work Package WP2 Document Type Deliverable Date 13 April 2020 Document Status Final version Acknowledgments & Disclaimer This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 824665. Neither the European Commission nor any person acting on behalf of the Commission is responsible for the use which might be made of the following information. The views expressed in this publication are the sole responsibility of the author and do not necessarily reflect the views of the European Commission. Reproduction and translation for non-commercial purposes are authorised, provided the source is acknowledged and the publisher is given prior notice and sent a copy. WP2 Case study: Endocrine disruptors i Abstract Endocrine disrupting chemicals (EDCs) are at the centre stage of a scientific and regulatory controversy. Given the complexities, ambiguities and particularly the uncertainties surrounding the hazards of EDCs, the precautionary principle is of utmost relevance to the case. Even the definition of EDCs remains much contested, as do the scientific processes and methods through which to identify them. On the one hand, there is considerable societal pressure to regulate ECDs ‘now’. On the other hand, this quick regulation is often impossible as the limited evidence available does not suffice in the context of traditional EU scientific risk assessment. -
Simultánní Stanovení Prenylflavonoidů a Isoflavonoidů Ve Chmelu a Pivu
KVASNY PRUM. Simultánní stanovení prenylflavonoidů a isoflavonoidů ve chmelu a pivu ... 59 / 2013 (2) 41 Simultánní stanovení prenylflavonoidů a isoflavonoidů ve chmelu a pivu metodou HPLC-DAD: Studie aplikace homogenátu zeleného chmele v pivovarském procesu Simultaneous Determination of Prenylflavonoids and Isoflavonoids in Hops and Beer by HPLC-DAD Method: Study of Green Hops Homogenate Application in the Brewing Process MARIE JURKOVÁ 1, Pavel ČEJKA 1, MILAN HOUŠKA 2, ALExANDR MIKYŠKA 1 1 Výzkumný ústav pivovarský a sladařský, a.s., Pivovarský ústav Praha, Lípová 15,120 44 Praha 2 / Research Institute of Brewing and Malting PLC, Brewing Institute Prague, Lípová 15, CZ – 120 44 Prague 2, Czech Republic 2 Výzkumný ústav potravinářský Praha / Food Research Institute Prague – Rádiová 7, CZ – 102 31, Prague 10, Czech Republic e-mail: [email protected] Jurková, M. – Čejka, P. – Houška, M. – Mikyška, A.: Simultánní stanovení prenylflavonoidů a isoflavonoidů ve chmelu a pivu metodou HPLC-DAD: Studie aplikace homogenátu zeleného chmele v pivovarském procesu. Kvasny Prum . 59, 2013, č . 2, s . 41–49 . Polyfenolové látky náležející do skupin prenylflavonoidů a isoflavonoidů mají účinky podporující zdraví a ochranu proti řadě civilizač- ních chorob . Působí jako antioxidanty, mají protirakovinné, antimikrobiální, protizánětlivé vlastnosti, některé jsou fytoestrogeny a půso- bí proti osteoporóze . Byla vypracována nová analytická metoda HPLC-DAD pro simultánní stanovení prenylflavonoidů (xanthohumol, isoxanthohumol, 8-prenylnaringenin, 6-prenylnaringenin) a isoflavonoidů (daidzein, genistein, formononetin a Biochanin A) ve chmelu a pivu a byla provedena studie dopadu chmelení homogenátem zeleného chmele stabilizovaného vysokým tlakem na obsah těchto látek v pivu . Vypracovaná metoda může najít uplatnění při monitorování obsahu bioaktivních flavonoidů ve chmelových surovinách a pivu . -
Highly Isoxanthohumol Enriched Hop Extract Obtained by Pressurized Hot
ACCEPTED MANUSCRIPT HIGHLY ISOXANTHOHUMOL ENRICHED HOP EXTRACT OBTAINED BY PRESSURIZED HOT WATER EXTRACTION (PHWE). CHEMICAL AND FUNCTIONAL CHARACTERIZATION. Alicia Gil-Ramíreza; José Antonio Mendiolab; Elena Arranza; Alejandro Ruíz- Rodrígueza; Guillermo Regleroa; Elena Ibáñez; Francisco R Marína* a Department of Characterization and production of New Foods. b Department of Bioactivity and Food Analysis. Institute of Food Science Research (CIAL), (Spanish National Research Council–Universidad Autónoma de Madrid), C/Nicolás Cabrera, 9. Campus de la Universidad Autónoma de Madrid, 28049 Madrid, Spain. * Corresponding author: [email protected] +34910017921 ACCEPTED MANUSCRIPT 1 ACCEPTED MANUSCRIPT ABSTRACT Hop (Humulus lupulus) is one of the richest natural sources of a prenylfalvonoids such as xanthohumol (XN), desmetylxanthohumol (DMX), isoxanthohumol (IX) or 8- prenylnaringenin (8-PN), being XN the most abundant of them in the raw material. So far, obtention of prenylflavonoids have been done by chemical synthesis or extraction with organic solvents, with no described methods for the isolation of IX, which has been reported to have anti-inflammatory properties. In this study, pressurized hot water extraction (PHWE) it is shown not only as effective method to extract some prenylflavonoids but to selectively change the relative amount of them, favoring the extraction of IX against XN. Thus, pressurized water extraction at 150ºC showed a high selectivity towards IX, being proposed as a method to enrich natural hop´s extracts in IX. On the other hand, the extracts thus obtained were chemically characterized and evaluated for their anti-inflammatory activity, which was higher than the expected by its content in IX. KEYWORDS: Prenylflavonoids, xanthohumol (XN), isoxanthohumol (IX), Pressurized HotACCEPTED Water Extraction (PHWE), MANUSCRIPT anti-inflamatory. -
Effects of Bisphenol a and Its Analogs on Reproductive Health: a Mini Review
View metadata, citation and similar papers at core.ac.uk brought to you by CORE HHS Public Access provided by CDC Stacks Author manuscript Author ManuscriptAuthor Manuscript Author Reprod Manuscript Author Toxicol. Author Manuscript Author manuscript; available in PMC 2019 August 11. Published in final edited form as: Reprod Toxicol. 2018 August ; 79: 96–123. doi:10.1016/j.reprotox.2018.06.005. Effects of Bisphenol A and its Analogs on Reproductive Health: A Mini Review Jacob Steven Siracusa1, Lei Yin1,2, Emily Measel1, Shenuxan Liang1, Xiaozhong Yu1,* 1.Department of Environmental Health Science, College of Public Health, University of Georgia, Athens, Georgia 30602 2.ReproTox Biotech LLC, Athens 30602, Georgia Abstract Known endocrine disruptor bisphenol A (BPA) has been shown to be a reproductive toxicant in animal models. Its structural analogs: bisphenol S (BPS), bisphenol F (BPF), bisphenol AF (BPAF), and tetrabromobisphenol A (TBBPA) are increasingly being used in consumer products. However, these analogs may exert similar adverse effects on the reproductive system, and their toxicological data are still limited. This mini-review examined studies on both BPA and BPA analog exposure and reproductive toxicity. It outlines the current state of knowledge on human exposure, toxicokinetics, endocrine activities, and reproductive toxicities of BPA and its analogs. BPA analogs showed similar endocrine potencies when compared to BPA, and emerging data suggest they may pose threats as reproductive hazards in animal models. While evidence based on epidemiological studies is still weak, we have utilized current studies to highlight knowledge gaps and research needs for future risk assessments. Keywords Bisphenol A; Bisphenol F; Bisphenol S; Bisphenol AF; Tetrabromobisphenol A; Reproductive toxicity 1. -
Exposure to Endocrine Disrupting Chemicals and Risk of Breast Cancer
International Journal of Molecular Sciences Review Exposure to Endocrine Disrupting Chemicals and Risk of Breast Cancer Louisane Eve 1,2,3,4,Béatrice Fervers 5,6, Muriel Le Romancer 2,3,4,* and Nelly Etienne-Selloum 1,7,8,* 1 Faculté de Pharmacie, Université de Strasbourg, F-67000 Strasbourg, France; [email protected] 2 Université Claude Bernard Lyon 1, F-69000 Lyon, France 3 Inserm U1052, Centre de Recherche en Cancérologie de Lyon, F-69000 Lyon, France 4 CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, F-69000 Lyon, France 5 Centre de Lutte Contre le Cancer Léon-Bérard, F-69000 Lyon, France; [email protected] 6 Inserm UA08, Radiations, Défense, Santé, Environnement, Center Léon Bérard, F-69000 Lyon, France 7 Service de Pharmacie, Institut de Cancérologie Strasbourg Europe, F-67000 Strasbourg, France 8 CNRS UMR7021/Unistra, Laboratoire de Bioimagerie et Pathologies, Faculté de Pharmacie, Université de Strasbourg, F-67000 Strasbourg, France * Correspondence: [email protected] (M.L.R.); [email protected] (N.E.-S.); Tel.: +33-4-(78)-78-28-22 (M.L.R.); +33-3-(68)-85-43-28 (N.E.-S.) Received: 27 October 2020; Accepted: 25 November 2020; Published: 30 November 2020 Abstract: Breast cancer (BC) is the second most common cancer and the fifth deadliest in the world. Exposure to endocrine disrupting pollutants has been suggested to contribute to the increase in disease incidence. Indeed, a growing number of researchershave investigated the effects of widely used environmental chemicals with endocrine disrupting properties on BC development in experimental (in vitro and animal models) and epidemiological studies.