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(: Nudibranchia) Goniobranchus annulatus

I I C

*** [email protected] 1392 : 1392 : ((( Heterobranch ))) ((( )))) ((( Opisthobranchia ))) Cladobranchia Anthobranchia ... Goniobranchus annulatus ... G. annulatus COI ... Anthobranchia .... 15151515 Archive Neighbor of _joining SID 100100100 Goniobranchus vibratus Goniobranchus annulatus ... . : ﻧﺮﻣﺘﻨﺎن، ﺷﻜﻤﭙﺎﻳﺎن، رده ﺑﻨﺪي، COI, ﺳﻮاﺣﻞ ﭼﺎﺑﻬﺎر

heterobranch . (2005 Opisthobranchia . . Nudibranchia Haszprunar & Huber,) 1988 *Corresponding author 77

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. . (Thompson, 2009) Nudibranchia Opisthobranchia Euthyneura ( 2000 ) Thollesson . 2005 (. Dinapoli , 2009 ) Pulmonata 16S rRNA sidegill slug ) pleurobranch Dorid COI ( c 658 bp . 2010 . ( COI ) Opisthobranchia Hebert et al. , ) Nudipleura ( 2003 ( ) Euthyneura Goniobranchus . 200 . (Guido et al. , 2006) : Dorid aeolid ( Anthobranchia ) dorid ( Guido et al., 2006) (Wollscheid (et ) ( Cladobranchia ) Goniobranchus Cladobranchia Odhner, 1934 .al. , 2001) Dendronotoidea Anthobranchia . Aeolidoidea . . Doridoidea Doridoidea Bathydoridoidea (. Wägele, 1989 ) 5 1391 Cladobranchia Anthobranchia .( 1 ) (. Wollscheid & Wägele, 1999) 3000 . . heterobranchs DNA . Sea ) 80 . ( slug . (Thollesson, 2000) 2% ) CTAB Archive 500 of SID . CTAB ,1.4M NaCl , 0.1% β_mercaptooethanol RS232C 5 20mM EDTA and 100mM TRIS-HCl pH8 ) DNA . _mercapthonal β 4/1 K DNA E_BOX_VX2/2M . 20 - . COI DNA ( 24:1 ) LCO1490f , HCO2198r DNA . (Galil et al. , 2010) DNA -20 . Ependorf 5331 1 78

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(Kumar et al. , 1994 ) 50 DNA 15 BioEdit Ver 7.0 ( Hall, 1999 ) MEGA Ver 5 . . ClustalX (Jeanmougin et al. , 1998) ,PCR 10 x DNA

Taq DNA polymeras dNTP Mgcl 2Buffer BioEdit . 95 5 PCR . Clustal X 38 DNA 1 95 . MEGA 1 45 Neighbor _joining 1 72 Nudibranch 15 . 10 72 . 1( ) PCR Mesogastropoda Littorina littorea clean up 1 . . 1: Goniobranchus annulatus , Iranian species KF 408221 Goniobranchus leopardus JQ 727847 Goniobranchus hintuanensis JQ 727845 Goniobranchus vibratus JQ 727859 Chromodoris ambiguus EF 535119 Durvilledoris similaris EF 535128 Hypselodoris bollandi JO 7278867 Hypselodoris apolegma JO 727886 Tambja blacki EF 142879 Tambja olivaria EF 142865 Tambja morosa EF 142868 Archive of SID 345038 Tambja ceutae AY Tambja sp. DQ 230999 Tambja eliora EF 142872 Austrodoris kerguelenensis GQ 292936 Doris kerguelenensis GQ 292036 Littorina littorea ( out group) GQ 845183

.

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G. annulatus Chromodoris annulata . ( 10 ) 100 Neighbor_joining 1(. ) Goniobranchus annulatus . Goniobranchus vibratus Pease, 1860 Chromodorididae Bergh, 1891 . G. annulatus (Synonym) . ( 2 ) Chromodoris annulata Eliot,1904 Chromodoris annulata Chromodorididae ( 1388 ) 100 Goniobranchus vibratus . Goniobranchus annulatus Archive of SID . =1 . Goniobranchus annulatus 1: . 4 3 2 :1 ( _ )

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Archive of SID . NJ COI 2: . Littorina littorea . replication 1000 . Goniobranchus annulatus Iranian species

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Chromodoris Mexichromis Hypselodoris ) . ( Glossodoris 89 Goniobranchus . Goniobranchus annulatus 15 . Nudibranchia Goniobranchus NJ . Chromodorididae . Dorid . dorid 2 Goniobranchus (G. annulatus ) Nudibranchia . vibratus Goniobranchus vibratus (Wägele, 1989;Wägele & Willan, 2000) . 18S ( 1999 ) Wägele Wollscheid 3(. ) 19 53 rDNA . chromodorid 16S rDNA ( 1999 a ) Thollesson Goniobranchus vibratus . 30 Cladobranchia . . 241 Nudibranchia Anthobranchia Wägele Wollscheid (. Wollscheid et al. , 2001 ) .(Bertsch & Johnson, 1982) . (1999 ) Chromodoris annulata . Anthobranchia Synonym ( 4 ) (G. annulatus ) Goniobranchus annulatus Goniobranchus vibratus . Chromodorididae NJ Chromodorididae . Archive of SID . 18S ( 2008 ) Wilson Turner 4(. 3 ) COI rDNA, 16S rDNA

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Goniobranchus vibratus 3: :1 . =1 . ( 2 ) G. annulatus 3 2 ( www.marinelifephotography.com ) G. annulatus Chromodoris annulata 4: ( www.nudipixel.net/locations/country/irArchive of) SID=1 . Rudman . H .apolegma Hypselodoris bollandi (1984 ) 2( ) Chromodoris Durvilledoris Hypselodoris Goniobranchus annulatus ( 1999 b ) Thollesson Hypselodoris Chromodoris . Hypselodoris Durvilledoris Chromodoris ambiguus Hypselodoris Polyceridae . Durvilledoris similaris Rocroi Bouchet .Tambja Burn, 1962 83

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another alien jellyfish from the Mediterranean Doridacea Polyceridae ( 2005 ) coast of Israel. Aquatic Invasions, 5(4):331– Nudipleura Euctenidiacea 340. (2006 ) Pola . Guido T. and Poppe Sheila P.T., 2006. The new Tambja classification of gastropods according to Bouchet Chromodorididae . & Rocroi, 2005; Philippines, Lapu City, Visaya Dorididae . 11P. (Rafinesque, 1815) Hall T.A., 1999. BIOEDIT: A user-friendly Doris kerguelenensis Austrodoris kerguelenensis biological sequence aligment editor and analysis 100 for Windows 95/98/NT. Nucleic Symposium Chromodorididae Series (London), 41:95-98 . Haszprunar G. and Huber G., 1990. On the central nervous system of Smeagolidae and Rhodopidae, two families questionably allied with the Gymnomorpha (: Euthyneura). Science and Zoology, 220:185-199. Hebert P.D.N., Cywinska A., Ball S.L. and . deWaard J.R., 2003. Biological identifications through DNA barcodes. Proceeding of the Royal Society of London, B, 270(1512):313–321. Bertsch H. and Scott J., 1982. Three new species Jeanmougin F., Thompson J.D., Gouy M., of dorid nudibranchs (Gastropoda: Higgins D.G. and Gibson T.J., 1998. Multiple Opisthobranchia) from the Hawaiian Islands. The sequence alignment with Clustal X. Trends. Veliger, 24(3):208-218. Biochemistry Science, 23:403–405. Boettger C., 1955. Die Taxonomie der euthyneuren Jörger K. M., Stöger I., Kano Y., Fukuda H., Schnecken. Zoologischer Anzeiger, 18:253-280. Knebelsberger T. and Schrödl M., 2010. On Bouchet P. and Rocroi J.P., 2005 . Classification the origin of Archive Acochlidia and other enigmatic ofand nomenclator SID of gastropod families. euthyneuran gastropods, with implications for International. Journal of Malacology, 47(1-2): the systematics of Heterobranchia. BMC 257-267. Evolutionary Biology, 10:320-323. Dinapoli A., 2009. Phylogeny andevolution of the Kumar S., Tamura K. and Nei M., 1994. MEGA: Heterobranchia (Mollusca, Gastropoda). Molecular Evolutionary Genetics Analysis Frankfurt am Main. Johann Wolfgang Goethe – software for microcomputers. Computer Universität als Dissertation angenommen. 176P. Applications in the Biosciences, 10(2):189–91. Galil B.S., Gershwin L.A., Douek J. and Minichev Y.S., 1970. On the origin and system of Rinkevich B., 2010. Marivagia stellatagen . et nudibranchiate (Mollusks, Gastropoda, sp. nov. (Scyphozoa: Rhizostomeae: Cepheidae), 84

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Opisthobranchia). Monitore Zoologico Italiano, mitochondrial 16S rRNA gene. Journal of Molluscan 4:169-182. Studies, 65: 335-353. Pelseneer P., 1893-1894. Recherches sur divers Thollesson M., 1999b. Phylogenetic analysis of opisthobranches. Memoires couronnes des Euthyneura (Gastropoda) by means of the 16S rRNA Savants strangers publics par I'Academie Royale gene. Proceedings of the Royal Society of London. des Sciences des Lettres et des Beaux - Arts de Series B: Biological Sciences, 266: 75–83. Belgique, 53:1-157. Thompson T.E., 2009. Feeding in nudibranch larvae. Pola M., Cervera J.L. and Gosliner T.M., 2006. Journal of the Marine Biological Association of the Taxonomic revision and phylogenetic analysis of United Kingdom, 38(2):239-248. the genus Tambja Burn, 1962 (Mollusca, Turner L.M. and Wilson N.G., 2007. Polyphyly Nudibranchia, Polyceridae), Zoologica Scripta, across oceans: A molecular phylogeny of the 35(5):491-530. Chromodorididae (Mollusca, Nudibranchia). Rudman W.B., 1984. The Chromodorididae Zoologica Scripta 37(1):23–42. (Opisthobranchia: Mollusca) of the Indo-West Wägele H., Willan R.C., 2000. On the phylogeny of Pacific: A review of the genera. Zoological the Nudibranchia. Zoological Journal of The Journal Of The Linnean Society, 81:115–273. Linnean Society, 130:83–181. Schmekel L., 1985. Aspects of evolution within the Wägele H., 1989. Die Gattung Bathydoris Bergh, 1884 opisthobranchs. In : Wilbur KM (ed) The (Gnathodoridacea) im phylogenetischen System der Mollusca. London .Academic Press, 221-267P. Nudibranchia (Opisthobranchia, Gastropoda). Tardy J., 1970. Contribution à l'etude des Zeitschrift Fur Zoologische Systematik Und métamorphoses chez les . Annales Evolutionsforschung, 27:273–281. des Sciences Naturelles Zoologie Biology, 12: Wollscheid E. and Wägele H., 1999. Initial results on 299-370. the molecular phylogeny of the Nudibranchia Thiele J., 1929-1935. Handbuch der Systematischen (Gastropoda, Opisthobranchia) based on 18S rDNA Weichtierkunde, 1154 P. data. Molecular Phylogenetics and Evolution, 13: Thollesson M., 2000. Increasing fidelity in 215-226. parsimony analysis of dorid nudibranchs by Wollscheid-Lengeling E., Boore J., Brown W. and differential weighting,Archive or a tale of two genes. Wägele of H., 2001. TheSID phylogeny of Nudibranchia Molecular Phylogenetics and Evolution, 16(2): (Opisthobranchia, Gastropoda, Mollusca) 161–172. reconstructed by three molecular markers. Thollesson M., 1999a. Phylogenetic analysis of dorid Organisms Diversity & Evolution, 1:241–256. nudibranchs (Gastropoda Doridacea) using the

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The first phylogenetic study of Goniobranchus annulatus (Mollusca : Nudibranchia) in subtidal area of Chabahar, Oman Sea, Iran:, based on sequence of cytochrome

oxidase C subunite I gene

Attaran Fariman G.* and Moosavipoor Y.

[email protected] Chabahar Maritime University (CMU), Faculty of Marine Sciences, Department of Marine Biology Chabahar, Iran

Received: March 2013 Accepted: July 2013

Keywords: Gasteropoda, CO1, Chabahar coast

Abstract Nudibranches are the largest group of Hetrerobranches, (a sub-branch of Opisthobranchia) and are widespread and cosmopolitan species. They vary in shape and colors and occur in all waters from intertidal to deep sea zones and from polar to the tropics regions. Nudibranches are subdivided in two main groups including Anthobranchia and Cladobranchia. In the present study, based onArchive morphology the svudied species wasof identified SID as Goniobranchus annulatus that belong to Anthrobranchia group. In molecular analysis the sequences of the partial mitochondrial COI gene of Goniobranchus annulatus species from Iran with 15 similar sequence of nudibrnaches from genbank were compared. Phylogenetic based on Neighbor_joining analyze showed a monophyly relationships between families of nudibranches and also revealed that Goniobranchus vibratus is a sister taxa to the Gonidbranchus annulatus from Iran Goniobranchus annulatus with 100% bootstrap support.

*Corresponding author

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