Orthopaedic Research Center 2012-2013 Report 2012-2013 Center Research Orthopaedic 2012-2013 Report Orthopaedic Research Center 2012-2013 Report 2012-2013 Center Research Orthopaedic 2012-2013 Report Preface It is my pleasure to present our 2012-2013 report from the Orthopaedic Research Center and the Orthopaedic Bioengineering Research Laboratory at Colorado State University. Our principal focus continues to be solving the signicant problems in equine musculoskeletal disease, as can be seen in this report, but we also continue to investigate questions relevant to human joint disease and techniques and devices for human osteoarthritis and articular cartilage repair when the technique can also potentially benet the horse. e increased number of translational projects and funding support from the National Institutes of Health (NIH) support our mission of helping both horses and humans. ere have been a number of notable projects in this regard. Evaluation of a combination of microfracture and an injectable self-assembling peptide (KLD) hydrogel on repair of articular cartilage defects in an equine model (funded by an NIH Program Grant) has shown that both microfracture and KLD augment repair, with microfrac- ture improving the quality of tissue and KLD improving the amount of ll and protecting against radiographic changes. is study has just accepted in the Journal of Bone and Joint Surgery. A collaborative study between Drs. Frisbie and McIlwraith, with Drs. Charlie Archer and Helen McCarthy at the University of Cardi resulted in improvement with cartilage-derived progenitor cells when they were autologous but not when they were allogeneic. e work with Dr. Jude Samulski at the University of North Carolina on Dr. Laurie Goodrich’s NIH KO8 grant on gene therapy (co-mentored by Drs. Samulski and McIlwraith) resulted in the ability to produce protein for six months, whereas previous work with an adenoviral vector only provided 30 months expression. e nal “proof of the pudding” in equine osteoarthritis is in its nal stages. Another NIH grant with Dr. Steve Trippel at the University of Indiana, in which Drs. Frisbie and McIlwraith are co-PIs on the subcontract involving gene transvected chondro- cytes and articular cartilage repair, is at the 12-month stage, and we have recently evaluated the repair arthroscopically with another six months to go in the horses, and the project will be completed in another six months. 2012-2013 Orthopaedic Research Center Research Report 1 While there have been some signicant publications Another move that greatly strengthens us is the from the horse/human collaborative projects in leading Orthopaedic Bioengineering Research Laboratory journals in the past two years, many projects addressing merged with the ORC, and is now designated as the equine-specic problems have been completed and pub- Bioengineering Laboratory within the ORC. OBRL lead- lished, and all this is detailed in the report. A comparative ers Drs. Tammy Donahue and Christian Puttlitz continue study of computed tomography and computed tomo- to do excellent work. Drs. Donahue and Ketul Popat graphic arthrography involving Dr. Brad Nelson during recently gained a $1.2 million research grant in conjunc- his surgical residency demonstrated that CT and CTR are tion with the Trinity College Centre for Bioengineering valuable methods for evaluating stie disease, especially (Ireland) and Queen’s University (Northern Ireland) when other diagnostic methods failed to detect the to address a growing problem related to knee injuries, source of lameness. Dr. Nelson also recently received the specically focusing on the so-tissue-to-bone interface. prestigious Storm Cat Award from the Grayson- Jockey Club Research Foundation. Dr. Frisbie’s work on standing Accomplishments at the ORC over the past two years arthroscopy of the stie has been published in a refereed are detailed in this report. ese accomplishments could journal and we held a course in this in 2013 at CSU, along not be achieved without our team of faculty and sta, as with an advanced focused arthroscopic surgery course. well as the excellent support of equine funding agencies We have continued to use both competitive research grant (Grayson-Jockey Club Research Foundation, American funding and discretionary income from donors to keep Quarter Horse Association, and United States Equestrian things aoat. is would not have been possible without Federation), corporate funding, and individual donors. our donors continuing to provide supplemental funding. With this help, we continue to achieve our goals and also make new ones as new clinical questions arise. A particular highlight of 2013 was the acquisition of a $6 million endowed Presidential Chair from John and Best wishes, Leslie Malone. is was the biggest gi we have ever received and is really going to put our Equine Sports Medicine program on solid footing. Our Equine Sports Medicine clinical arm of the Orthopaedic Research Wayne McIlwraith Center has residents in all three years, and remains the only equine sports medicine and rehabilitation resi- dency program in the country. We also are particularly delighted to welcome Dr. Melinda Story to our team. 2 2012-2013 Orthopaedic Research Center Research Report TABLE OF CONTENTS Preface .....................................................................1 Research Techniques Available Mission ....................................................................6 at the Orthopaedic Research Center .................. 60 Research Focuses of the Orthopaedic Research Research Techniques Available Center ......................................................................8 at the Orthopaedic Bioengineering Research Laboratory ............................................62 Research Focuses at the Orthopaedic Bioengi- neering Research Laboratory ................................8 Scientific Publications and Presentations ......... 64 Musculoskeletal Research Program ................... 10 Funded Research Projects ................................... 83 School of Biomedical Engineering .....................11 Revenue and Expenses, FY12 to FY13 ................ 88 Advisory Board 2011 ........................................... 12 News 2012-2013 Editorial and Scientific Advisory Boards of ORC Equine Surgeon Honored for Research That Faculty 2012-2013 ................................................ 13 Helps Horses and Humans .........................91 Our Donors........................................................... 14 Malone Foundation Gives $6 million to Equine Sports Medicine and Faculty ...................................................................18 Rehabilitation Program ..............................91 College of Veterinary Medicine and Horse Sense Documentary ...............................91 Biomedical Sciences ....................................20 Honors and Awards 2012-2013 ...........................92 College of Engineering .....................................26 Professional Associations ....................................93 Affiliate Faculty .................................................30 Summary of Research Projects 2010-2011 ........ 95 Collaborators .....................................................32 Focus 1. Musculoskeletal Tissue Healing 2012-2013 Post Doctoral Fellows .....................38 Autologous Platelet Enhanced Fibrin Equine Sports Medicine Residents ..................39 (APEF) Compared to APEF with Culture Equine Surgery Residents and Expanded Bone Marrow Derived M.S. Graduate Students ..............................40 Mesenchymal Stem Cells (BMDMSCs) to Enhance Cartilage Repair in an Equine Ph.D. Graduate Students ..................................41 Model ...........................................................96 Visiting Reseachers ............................................43 Effects of Equine Bone Marrow Aspirate Research Associates ...........................................44 Volume on the Isolation, Proliferation, and Differentiation Potential of Coordinators and Administrative Staff ...........45 Mesenchymal Stem Cells ............................98 ORC Student Hourlies and Volunteers ............46 Nanoindentation of Human Graduate Students Placement ............................ 47 Meniscal Tissue ..........................................100 Surgery Residents Supervised (and Outcome).. 49 Phenomenological Consequences of Program Synopsis ................................................50 Sectioning and Bathing on Passive Muscle Mechanics of the New Zealand White History ...............................................................51 Rabbit Tibialis Anterior ............................102 Research Activities .............................................52 Dynamic Compression Plate (DCP) Impact ................................................................52 Fixation of Propagating Medial Condylar Fractures of the Third Metacarpal/ Program Trends .................................................53 Metatarsal Bone in 30 Racehorses: Program Goals ..................................................53 Retrospective Analysis (1990–2005) ........103 Goals Accomplished 2012-2013 ...............53 Science and Animal Models of Marrow Current Goals ............................................54 Stimulation for Cartilage Repair ..............106 Research Goals ..................................................54 Research Goals Achieved 2012-2013 ........54 2012-2013 Orthopaedic Research Center Research Report 3 TABLE OF CONTENTS Current Trends in