Human Cystatin SA / CST2 (His Tag)

Catalog Number: 11567-H08H

General Information SDS-PAGE:

Gene Name Synonym:

CST2; MGC71924 Protein Construction:

A DNA sequence encoding the human CST2 (NP_001313.1) (Met 1-Ala 141) was fused with a polyhistidine tag at the C-terminus. Source: Human Expression Host: HEK293 Cells QC Testing

Purity: > 98 % as determined by SDS-PAGE Endotoxin: Protein Description < 1.0 EU per μg of the protein as determined by the LAL method Cystatin-SA, also known as Cystatin-2, Cystatin-S5 and CST2, is a Stability: secreted protein which belongs to thecystatin family. Cystatin-2 / CST2 is expressed in submandibular and sublingual saliva but not in parotid saliva Samples are stable for up to twelve months from date of receipt at -70 ℃ (at protein level). It is also expressed in submandibular gland and parotid gland. The cystatin superfamily encompasses that contain multiple Predicted N terminal: Trp 21 cystatin-like sequences. Some of the members are active cysteine Molecular Mass: protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, The secreted recombinant human CST2 consists of 132 amino acids and including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. migrates as an approximately 16 kDa band in SDS-PAGE under reducing The type 2 cystatin proteins are a class of cysteine proteinase inhibitors conditions as predicted. found in a variety of human fluids and secretions, where they appear to provide protective functions. The CST1, CST2, CST4, and CST5 are Formulation: expressed in differential, tissue-specific patterns. Expression of CST2 and CST5 is restricted to the submandibular and parotid glands, while CST1 Lyophilized from sterile PBS, pH 7.4 and CST4 are expressed in these tissues and in the lacrimal gland. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as References protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special 1.Saitoh E., et al.,(1987), Human cysteine-proteinase inhibitors: nucleotide requirements. sequence analysis of three members of the cystatin family. Gene 61:329-338. 2.Zhang Z., et al., (2004), Signal peptide prediction based on Usage Guide analysis of experimentally verified cleavage sites.Protein Sci. 13:2819- 2824. 3.Deloukas P., et al.,(2001), The DNA sequence and comparative Storage: analysis of human 20.Nature 414:865-871.

Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage.

Avoid repeated freeze-thaw cycles.

Reconstitution:

Detailed reconstitution instructions are sent along with the products.

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