Evaluation of Several Pre-Clinical Tools for Identifying Characteristics
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Purdue University Purdue e-Pubs Open Access Theses Theses and Dissertations 12-2016 Evaluation of several pre-clinical tools for identifying characteristics associated with limb bone fracture in thoroughbred racehorses Anthony Nicholas Corsten Purdue University Follow this and additional works at: https://docs.lib.purdue.edu/open_access_theses Part of the Veterinary Medicine Commons Recommended Citation Corsten, Anthony Nicholas, "Evaluation of several pre-clinical tools for identifying characteristics associated with limb bone fracture in thoroughbred racehorses" (2016). Open Access Theses. 841. https://docs.lib.purdue.edu/open_access_theses/841 This document has been made available through Purdue e-Pubs, a service of the Purdue University Libraries. Please contact [email protected] for additional information. EVALUATION OF SEVERAL PRE-CLINICAL TOOLS FOR IDENTIFYING CHARACTERISTICS ASSOCIATED WITH LIMB BONE FRACTURE IN THOROUGHBRED RACEHORSES by Anthony N. Corsten A Thesis Submitted to the Faculty of Purdue University In Partial Fulfillment of the Requirements for the degree of Master of Science in Basic Medical Sciences Department of Basic Medical Sciences West Lafayette, Indiana December 2016 ii THE PURDUE UNIVERSITY GRADUATE SCHOOL STATEMENT OF THESIS APPROVAL Dr. Russell P. Main, Chair Department of Basic Medical Sciences Dr. Timothy B. Lescun Department of Veterinary Clinical Sciences Dr. Joseph M. Wallace Department of Biomedical Engineering Approved by: Dr. Laurie A. Jaeger Head of the Departmental Graduate Program iii TABLE OF CONTENTS TABLE OF FIGURES ........................................................................................................ v LIST OF ABBREVIATIONS ............................................................................................ vi ABSTRACT ..................................................................................................................... viii 1. INTRODUCTION ....................................................................................................... 1 1.1 The Problem of Racehorse Fractures ........................................................................ 1 1.2 Factors Related to Overt Fractures and Stress Fractures in Racehorses ................... 2 1.3 Introduction to Pre-Clinical Modalities..................................................................... 3 2. METHODS ..................................................................................................................... 6 2.1 Collection and Preparation of Samples ..................................................................... 6 2.2 Horse Sample Demographics and Fracture Groups .................................................. 7 2.3 Sample Testing Pipeline ............................................................................................ 9 2.4 Testing Site Selection .............................................................................................. 10 2.5 Osteoprobe .............................................................................................................. 11 2.6 Biodent .................................................................................................................... 12 2.7 Raman Spectroscopy ............................................................................................... 14 2.8 Radiographs ............................................................................................................. 16 2.9 Peripheral Quantitative Computed Tomography (pQCT) ....................................... 18 2.10 MATLAB Data Organization ............................................................................... 22 2.11 Statistical Testing .................................................................................................. 23 3. RESULTS ..................................................................................................................... 25 3.1 Osteoprobe .............................................................................................................. 25 3.2 Biodent .................................................................................................................... 27 3.3 Linear Correlations Between Reference Point Indentation Devices ....................... 29 3.4 Raman Spectroscopy ............................................................................................... 31 3.5 pQCT ....................................................................................................................... 35 4. DISCUSSION ............................................................................................................... 38 4.1 pQCT ....................................................................................................................... 39 iv 4.1.1 Bone Mineral Content and Bone Mineral Density ........................................... 40 4.1.2 The Effect of Geometric Properties on Fracture Susceptibility ....................... 42 4.2 Raman Spectroscopy Analysis ................................................................................ 43 4.2.1 Mineral-to-Matrix Ratio & The Hypermineralization Theory ......................... 43 4.2.2 Bone Remodeling Rate ..................................................................................... 44 4.2.3 Carbonate Substitution ..................................................................................... 46 4.3 Reference Point Indentation .................................................................................... 46 4.3.1 Fracture Risk Analysis and Dorsal Metacarpal Disease ................................... 46 4.3.2 Comparison of Reference Point Indentation Devices....................................... 49 5. CONCLUSIONS AND FUTURE DIRECTIONS ....................................................... 51 REFERENCES ................................................................................................................. 55 APPENDIX ....................................................................................................................... 59 v TABLE OF FIGURES Figure 1. Equine forelimb anatomy. ................................................................................... 7 Figure 2. Tested locations for different modalities. .......................................................... 10 Figure 3. Typical report from the Osteoprobe. ................................................................. 11 Figure 4. Example output from the Biodent ..................................................................... 13 Figure 5. Typical successful and erroneous Raman spectroscopy output ........................ 15 Figure 6. Radiographs of four fractured MC3’s. .............................................................. 17 Figure 7.Typical pQCT output .......................................................................................... 20 Figure 8. Significant group level results for reference point indentation. ........................ 28 Figure 9.Lateral, dorsal and medial comparisons for reference point indentation ........... 29 Figure 10. Correlations between reference point indentation devices ............................. 30 Figure 11. Significant group level results for Raman spectroscopy ................................. 33 Figure 12. Significant interaction effects for Amide I mineral:matrix ............................ 33 Figure 13. Significant interaction effects for Amide III mineral:matrix .......................... 34 Figure 14. Significant interaction effects for CH2 wag mineral:matrix ............................ 34 vi LIST OF ABBREVIATIONS Abbreviation Meaning MC3 Third Metacarpal SSMD (Proximal Forelimb) Sesamoid LB Long Bone BMS Bone Material Strength BES Balanced Electrolytic Solution TB Thoroughbred SB Standardbred QH Quarter Horse DP Dorsal-Palmar LM Lateral-Medial 1st ID First Indentation Distance TID Total Indentation Distance IDI Indentation Distance Increase CID Creep Indentation Distance ED Energy Dissipated US Unloading Slope LS Loading Slope TDD Touchdown Distance BMD Bone Mineral Density BMC Bone Mineral Content TRAB Trabecular CORT Cortical (for Diaphysis) SUBCORT Cortical (for Metaphysis) TOT Total MORLM,W Section Modulus across the Lateral Medial Axis, Weighted vii MORDP,W Section Modulus across the Dorsal Palmar Axis, Weighted MORP,W Polar Section Modulus, Weighted FWHM Full-Width at Half-Maximum DMD Dorsal Metacarpal Disease viii ABSTRACT Author: Corsten, Anthony N., M.S. BMS Institution: Purdue University. Degree Received: Master of Science in Basic Medical Sciences, Fall 2016. Title: Evaluation of Several Pre-Clinical Tools for Identifying Characteristics Associated with Limb Bone Fracture in Thoroughbred Racehorses Major Professor: Dr. Russell Main, Ph.D. Catastrophic skeletal fractures in racehorses are devastating not only to the animals, owners and trainers, but also to the perception of the sport in the public eye. The majority of these fatal accidents are unlikely to be due to chance, but are rather an end result failure from stress fractures. Stress fractures are overuse injuries resulting from an accumulation of bone tissue damage over time. Because stress fractures are pathological, it is possible that overt fractures can be predicted and prevented. In this study, third metacarpals (MC3) from 33 thoroughbred racehorse comprised of 8 non-fractured controls and 25 horses that experienced fracture of some limb bone were evaluated for correlative factors for fracture using reference