AC27 Doc. 12.4 (Rev.1) – P. 1 Original Language
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Extreme Miniaturization of a New Amniote Vertebrate and Insights Into the Evolution of Genital Size in Chameleons
www.nature.com/scientificreports OPEN Extreme miniaturization of a new amniote vertebrate and insights into the evolution of genital size in chameleons Frank Glaw1*, Jörn Köhler2, Oliver Hawlitschek3, Fanomezana M. Ratsoavina4, Andolalao Rakotoarison4, Mark D. Scherz5 & Miguel Vences6 Evolutionary reduction of adult body size (miniaturization) has profound consequences for organismal biology and is an important subject of evolutionary research. Based on two individuals we describe a new, extremely miniaturized chameleon, which may be the world’s smallest reptile species. The male holotype of Brookesia nana sp. nov. has a snout–vent length of 13.5 mm (total length 21.6 mm) and has large, apparently fully developed hemipenes, making it apparently the smallest mature male amniote ever recorded. The female paratype measures 19.2 mm snout–vent length (total length 28.9 mm) and a micro-CT scan revealed developing eggs in the body cavity, likewise indicating sexual maturity. The new chameleon is only known from a degraded montane rainforest in northern Madagascar and might be threatened by extinction. Molecular phylogenetic analyses place it as sister to B. karchei, the largest species in the clade of miniaturized Brookesia species, for which we resurrect Evoluticauda Angel, 1942 as subgenus name. The genetic divergence of B. nana sp. nov. is rather strong (9.9‒14.9% to all other Evoluticauda species in the 16S rRNA gene). A comparative study of genital length in Malagasy chameleons revealed a tendency for the smallest chameleons to have the relatively largest hemipenes, which might be a consequence of a reversed sexual size dimorphism with males substantially smaller than females in the smallest species. -
(Equatorial Spitting Cobra) Venom a P
The Journal of Venomous Animals and Toxins including Tropical Diseases ISSN 1678-9199 | 2011 | volume 17 | issue 4 | pages 451-459 Biochemical and toxinological characterization of Naja sumatrana ER P (Equatorial spitting cobra) venom A P Yap MKK (1), Tan NH (1), Fung SY (1) RIGINAL O (1) Department of Molecular Medicine, Center for Natural Products and Drug Research (CENAR), Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. Abstract: The lethal and enzymatic activities of venom from Naja sumatrana (Equatorial spitting cobra) were determined and compared to venoms from three other Southeast Asian cobras (Naja sputatrix, Naja siamensis and Naja kaouthia). All four venoms exhibited the common characteristic enzymatic activities of Asiatic cobra venoms: low protease, phosphodiesterase, alkaline phosphomonoesterase and L-amino acid oxidase activities, moderately high acetylcholinesterase and hyaluronidase activities and high phospholipase A2. Fractionation of N. sumatrana venom by Resource® S cation exchange chromatography (GE Healthcare, USA) yielded nine major protein peaks, with all except the acidic protein peak being lethal to mice. Most of the protein peaks exhibit enzymatic activities, and L-amino acid oxidase, alkaline phosphomonoesterase, acetylcholinesterase, 5’-nucleotidase and hyaluronidase exist in multiple forms. Comparison of the Resource® S chromatograms of the four cobra venoms clearly indicates that the protein composition of N. sumatrana venom is distinct from venoms of the other two spitting cobras, N. sputatrix (Javan spitting cobra) and N. siamensis (Indochinese spitting cobra). The results support the revised systematics of the Asiatic cobra based on multivariate analysis of morphological characters. The three spitting cobra venoms exhibit two common features: the presence of basic, potentially pharmacologically active phospholipases A2 and a high content of polypeptide cardiotoxin, suggesting that the pathophysiological actions of the three spitting cobra venoms may be similar. -
Review of Species Selected at SRG 45 Following Working Group Recommendations on Reptiles and One Scorpion from Benin, Ghana and Togo
Review of species selected at SRG 45 following working group recommendations on reptiles and one scorpion from Benin, Ghana and Togo (Version edited for public release) A report to the European Commission Directorate General E - Environment ENV.E.2. – Environmental Agreements and Trade by the United Nations Environment Programme World Conservation Monitoring Centre November, 2008 UNEP World Conservation Monitoring Centre 219 Huntingdon Road Cambridge CB3 0DL CITATION United Kingdom UNEP-WCMC (2008). Review of species selected at SRG 45 following working group recommendations on reptiles Tel: +44 (0) 1223 277314 and one scorpion from Benin, Ghana and Togo. A Report Fax: +44 (0) 1223 277136 to the European Commission. UNEP-WCMC, Cambridge. Email: [email protected] Website: www.unep-wcmc.org PREPARED FOR ABOUT UNEP-WORLD CONSERVATION The European Commission, Brussels, Belgium MONITORING CENTRE The UNEP World Conservation Monitoring Centre DISCLAIMER (UNEP-WCMC), based in Cambridge, UK, is the The contents of this report do not necessarily reflect specialist biodiversity information and assessment the views or policies of UNEP or contributory centre of the United Nations Environment organisations. The designations employed and the Programme (UNEP), run cooperatively with WCMC presentations do not imply the expressions of any 2000, a UK charity. The Centre's mission is to opinion whatsoever on the part of UNEP, the evaluate and highlight the many values of European Commission or contributory organisations biodiversity and put authoritative biodiversity concerning the legal status of any country, territory, knowledge at the centre of decision-making. city or area or its authority, or concerning the Through the analysis and synthesis of global delimitation of its frontiers or boundaries. -
OCS Study BOEM 2017-024
OCS Study BOEM 2017-024 Deepwater Reconnaissance of Potentially Sensitive Biological Features Surrounding Shelf-Edge Topographical Banks in the Northern Gulf of Mexico U.S. Department of the Interior Bureau of Ocean Energy Management Gulf of Mexico OCS Region OCS Study BOEM 2017-024 Deepwater Reconnaissance of Potentially Sensitive Biological Features Surrounding Shelf-Edge Topographical Banks in the Northern Gulf of Mexico Author Paul Sammarco Prepared under BOEM Contract M11AC00005 by Louisiana Universities Marine Consortium 8124 Highway 56 Baton Rouge, LA 70344-2110 Published by U.S. Department of the Interior New Orleans, LA Bureau of Ocean Energy Management February 2017 Gulf of Mexico OCS Region DISCLAIMER This report was prepared under contract between the Bureau of Ocean Energy Management (BOEM) and Louisiana Universities Marine Consortium (LUMCON). This report has been technically reviewed by BOEM, and it has been approved for publication. Approval does not necessarily signify that the contents reflect the views and policies of BOEM, nor does mention of trade names or commercial products constitute endorsement or recommendation for use. REPORT AVAILABILITY To download a PDF file of this Gulf of Mexico OCS Region report, go to the U.S. Department of the Interior, Bureau of Ocean Energy Management, Environmental Studies Program Information System website and search on OCS Study BOEM 2017-024. This report can be viewed at select Federal Depository Libraries. It can also be obtained from the National Technical Information Service; the contact information is below. U.S. Department of Commerce National Technical Information Service 5301 Shawnee Rd. Springfield, Virginia 22312 Phone: (703) 605-6000, 1(800)553-6847 Fax: (703) 605-6900 Website: http://www.ntis.gov/ CITATION Sammarco, Paul W. -
Conservation Status of Animal Species Used by Indigenous Traditional Medicine Practitioners in Ogbomoso, Oyo State
Journal of Complementary and Alternative Medical Research 3(4): 1-8, 2017; Article no.JOCAMR.36018 ISSN: 2456-6276 Conservation Status of Animal Species Used by Indigenous Traditional Medicine Practitioners in Ogbomoso, Oyo State J. Ebele Ajagun 1* and E. Caesar Anyaku 2 1Medicinal Plant Unit, Bioresources Development Centre, National Biotechnology Development Agency, Ogbomoso, Oyo State, Nigeria. 2Veterinary Unit, Bioresources Development Centre, National Biotechnology Development Agency Ogbomoso, Nigeria. Authors’ contributions This work was carried out in collaboration between both authors. Author JEA designed the study, performed the statistical analysis, wrote the protocol, and wrote the first draft of the manuscript. Author ECA took part in the survey, managed the literature searches and contributed to the first draft of the manuscript. Both authors read and approved the final manuscript. Article Information DOI: 10.9734/JOCAMR/2017/36018 Editor(s): (1) Francisco Cruz-Sosa, Metropolitan Autonomous University Iztapalapa Campus Av. San Rafael Atlixco, Mexico. Reviewers: (1) M. Fawzi Mahomoodally, University of Mauritius, Mauritius. (2) Nwachukwu Francis Chukwuedozie, Nigeria Police Academy, Nigeria. Complete Peer review History: http://www.sciencedomain.org/review-history/21007 Received 8th August 2017 Accepted 7th September 2017 Original Research Article Published 15 th September 2017 ABSTRACT Aim: To document the indigenous knowledge of fauna species used in traditional medicine practices and to establish their conservational status. Study Design: A questionnaire guided survey of the traditional uses of fauna species by the indigenous people of Ogbomoso, Oyo State. Place and Duration of Study: Bioresources Development Centre, Ogbomoso, Oyo State, Nigeria between March and December, 2016. Methodology: A total of 43 participants were interviewed during the survey and constituted 4 hunters, 19 traditional medicine practitioner (TMP) and 20 trado-herbal traders (THT) as the study population. -
Literature Cited in Lizards Natural History Database
Literature Cited in Lizards Natural History database Abdala, C. S., A. S. Quinteros, and R. E. Espinoza. 2008. Two new species of Liolaemus (Iguania: Liolaemidae) from the puna of northwestern Argentina. Herpetologica 64:458-471. Abdala, C. S., D. Baldo, R. A. Juárez, and R. E. Espinoza. 2016. The first parthenogenetic pleurodont Iguanian: a new all-female Liolaemus (Squamata: Liolaemidae) from western Argentina. Copeia 104:487-497. Abdala, C. S., J. C. Acosta, M. R. Cabrera, H. J. Villaviciencio, and J. Marinero. 2009. A new Andean Liolaemus of the L. montanus series (Squamata: Iguania: Liolaemidae) from western Argentina. South American Journal of Herpetology 4:91-102. Abdala, C. S., J. L. Acosta, J. C. Acosta, B. B. Alvarez, F. Arias, L. J. Avila, . S. M. Zalba. 2012. Categorización del estado de conservación de las lagartijas y anfisbenas de la República Argentina. Cuadernos de Herpetologia 26 (Suppl. 1):215-248. Abell, A. J. 1999. Male-female spacing patterns in the lizard, Sceloporus virgatus. Amphibia-Reptilia 20:185-194. Abts, M. L. 1987. Environment and variation in life history traits of the Chuckwalla, Sauromalus obesus. Ecological Monographs 57:215-232. Achaval, F., and A. Olmos. 2003. Anfibios y reptiles del Uruguay. Montevideo, Uruguay: Facultad de Ciencias. Achaval, F., and A. Olmos. 2007. Anfibio y reptiles del Uruguay, 3rd edn. Montevideo, Uruguay: Serie Fauna 1. Ackermann, T. 2006. Schreibers Glatkopfleguan Leiocephalus schreibersii. Munich, Germany: Natur und Tier. Ackley, J. W., P. J. Muelleman, R. E. Carter, R. W. Henderson, and R. Powell. 2009. A rapid assessment of herpetofaunal diversity in variously altered habitats on Dominica. -
Quantitative Characterization of the Hemorrhagic, Necrotic, Coagulation
Hindawi Journal of Toxicology Volume 2018, Article ID 6940798, 8 pages https://doi.org/10.1155/2018/6940798 Research Article Quantitative Characterization of the Hemorrhagic, Necrotic, Coagulation-Altering Properties and Edema-Forming Effects of Zebra Snake (Naja nigricincta nigricincta)Venom Erick Kandiwa,1 Borden Mushonga,1 Alaster Samkange ,1 and Ezequiel Fabiano2 1 School of Veterinary Medicine, Faculty of Agriculture and Natural Resources, Neudamm Campus, University of Namibia, P. Bag 13301, Pioneers Park, Windhoek, Namibia 2Department of Wildlife Management and Ecotourism, Katima Mulilo Campus, Faculty of Agriculture and Natural Resources, University of Namibia, P. Bag 1096, Ngweze, Katima Mulilo, Namibia Correspondence should be addressed to Alaster Samkange; [email protected] Received 30 May 2018; Revised 5 October 2018; Accepted 10 October 2018; Published 24 October 2018 Academic Editor: Anthony DeCaprio Copyright © 2018 Erick Kandiwa et al. Tis is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Tis study was designed to investigate the cytotoxicity and haemotoxicity of the Western barred (zebra) spitting cobra (Naja nigricincta nigricincta) venom to help explain atypical and inconsistent reports on syndromes by Namibian physicians treating victims of human ophidian accidents. Freeze-dried venom milked from adult zebra snakes was dissolved in phosphate bufered saline (PBS) for use in this study. Haemorrhagic and necrotic activity of venom were studied in New Zealand albino rabbits. Oedema-forming activity was investigated in 10-day-old Cobb500 broiler chicks. Procoagulant and thrombolytic activity was investigated in adult Kalahari red goat blood in vitro. -
Description of a New Flat Gecko (Squamata: Gekkonidae: Afroedura) from Mount Gorongosa, Mozambique
See discussions, stats, and author profiles for this publication at: https://www.researchgate.net/publication/320043814 Description of a new flat gecko (Squamata: Gekkonidae: Afroedura) from Mount Gorongosa, Mozambique Article in Zootaxa · September 2017 DOI: 10.11646/zootaxa.4324.1.8 CITATIONS READS 2 531 8 authors, including: William R Branch Jennifer Anna Guyton Nelson Mandela University Princeton University 250 PUBLICATIONS 4,231 CITATIONS 7 PUBLICATIONS 164 CITATIONS SEE PROFILE SEE PROFILE Andreas Schmitz Michael Barej Natural History Museum of Geneva Museum für Naturkunde - Leibniz Institute for Research on Evolution and Biodiver… 151 PUBLICATIONS 2,701 CITATIONS 38 PUBLICATIONS 274 CITATIONS SEE PROFILE SEE PROFILE Some of the authors of this publication are also working on these related projects: Monitoring and Managing Biodiversity Loss in South-East Africa's Montane Ecosystems View project Ad hoc herpetofauna observations View project All content following this page was uploaded by Jennifer Anna Guyton on 27 September 2017. The user has requested enhancement of the downloaded file. Zootaxa 4324 (1): 142–160 ISSN 1175-5326 (print edition) http://www.mapress.com/j/zt/ Article ZOOTAXA Copyright © 2017 Magnolia Press ISSN 1175-5334 (online edition) https://doi.org/10.11646/zootaxa.4324.1.8 http://zoobank.org/urn:lsid:zoobank.org:pub:B4FF9A5F-94A7-4E75-9EC8-B3C382A9128C Description of a new flat gecko (Squamata: Gekkonidae: Afroedura) from Mount Gorongosa, Mozambique WILLIAM R. BRANCH1,2,13, JENNIFER A. GUYTON3, ANDREAS SCHMITZ4, MICHAEL F. BAREJ5, PIOTR NASKRECKI6,7, HARITH FAROOQ8,9,10,11, LUKE VERBURGT12 & MARK-OLIVER RÖDEL5 1Port Elizabeth Museum, P.O. Box 13147, Humewood 6013, South Africa 2Research Associate, Department of Zoology, Nelson Mandela University, P.O. -
Subgenus: Naja, Afronaja, Boulengerina and Uraeus)
Toxins 2019, 11, 116; doi: 10.3390/toxins11020116 www.mdpi.com/journal/toxins S1 of S2 Supplementary Materials: Distribution of Secretory Phospholipases A2 in the Venoms of Afro-Asian Cobras (Subgenus: Naja, Afronaja, Boulengerina and Uraeus) Choo Hock Tan, Kin Ying Wong, Nget Hong Tan, Tzu Shan Ng and Kae Yi Tan Figure 1. Time-dependent pH changes in acidimetric assay for the venoms of four subgenera of cobra. (A) Naja, (B) Afronaja, (C) Boulengerina, and (D) Uraeus. Hydrolysis of phospholipids by phospholipase A2 released fatty acids that reduced the suspension pH time-dependently. Toxins 2019, 11, 116; doi: 10.3390/toxins11020116 www.mdpi.com/journal/toxins S2 of S2 Figure S2. Time-dependent absorbance changes in colorimetric assay for the venoms of four subgenera of cobra. (A) Naja, (B) Afronaja, (C) Boulengerina, and (D) Uraeus. Changes in absorbance were due to the hydrolysis of the synthetic chromogenic substrate (NOBA), corresponding to the enzymatic activity of phospholipases A2 in the venoms. Toxins 2019, 11, 116; doi: 10.3390/toxins11020116 www.mdpi.com/journal/toxins S3 of S2 Table S1. Relative abundances of snake venom phospholipase A2 of 12 cobra species (Genus: Naja). Relative Subgenus Cobra Source Abundance of Method of Protein Identification References of Naja Species PLA2 (%) Bottom up proteomic: RP-HPLC, in-gel digestion, MALDI TOF/TOF and nano-ESI-LCMS/MS Naja naja Latoxan (Pakistan) 14.24 [1] Abundance calculation: gel densitometry x peak area under curve of chromatographic fraction Bottom up proteomic: RP-HPLC, in-gel -
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ISSN 1198-6727 Fisheries Centre Research Reports 2011 Volume 19 Number 2 Tracking the international trade of seahorses (Hippocampus species) Fisheries Centre, University of British Columbia, Canada Tracking the international trade of seahorses ( Hippocampus species) by Melissa Evanson, Sarah J. Foster, Stefan Wiswedel & Amanda C. J. Vincent Fisheries Centre Research Reports 19(2) 104 pages © published 2011 by The Fisheries Centre, University of British Columbia 2202 Main Mall Vancouver, B.C., Canada, V6T 1Z4 ISSN 1198-6727 Fisheries Centre Research Reports 19(2) 2011 TRACKING THE INTERNATIONAL TRADE IN SEAHORSES (HIPPOCAMPUS SPECIES ) by Melissa Evanson, Sarah J. Foster, Stefan Wiswedel & Amanda C. J. Vincent CONTENTS Page DIRECTOR ’S FOREWORD ......................................................................................................................................... 1 ABSTRACT ............................................................................................................................................................... 2 INTRODUCTION ....................................................................................................................................................... 3 METHODS ............................................................................................................................................................... 5 Trade Data Sources...................................................................................................................................... 5 Trade -
4 CITES Listed Species
Dutch Caribbean Species of High Conservation Value 4 CITES listed species 4.1 Notes on CITES Species CITES (the Convention on International Trade in Endangered Species of Wild Fauna and Flora) is an international agreement between governments. Its aim is to ensure that international trade in specimens of wild animals and plants does not threaten their survival. In the CITES legislation organisms are classified in 3 categories depending on how endangered they are thought to be, or the degree of protection they are thought to need (CITES-Website 2012); ! Appendix I includes species threatened with extinction. Trade in specimens of these species is permitted only in exceptional circumstances. Import and export or re-export permits are required, issued by the Management Authority of the State of Import, Export or Re-Export. ! Appendix II includes species where trade is allowed but restricted. An export or re-export certificate is required, but no import permit is needed unless required by national law. ! Appendix III are species in which trade is allowed but monitored. An export permit must be issued by the Management Authority of the State that included the species in Appendix III. 4.2 Summary data CITES I CITES II CITES I CITES II CITES Dutch Caribbean 15 189 Saba 16 153 Marine 12 1120 Marine 5 115 Terrestrial 3 68 Terrestrial 1 36 Aruba 7 124 St Eustatius 160 159 Marine 4 102 Marine 5 115 Terrestrial 3 21 Terrestrial 1 43 Bonaire 17 153 St Maarten 16 152 Marine 5 115 Marine 5 115 Terrestrial 2 31 Terrestrial 1 33 Curaçao 17 158 Marine 6 115 Terrestrial 1 36 CITES listed species 31 November 2012 Dutch Caribbean Species of High Conservation Value 4.3 CITES I 4.3.1 Marine CITES Appendix I species Common Group Name English Name Scientific name Notes Aruba Bonaire Curaçao Saba Eustatius St. -
Revision of the Antipatharia (Cnidaria: Anthozoa)
ZM 75 343-370 | 17 (opresko) 12-01-2007 07:55 Page 343 Revision of the Antipatharia (Cnidaria: Anthozoa). Part I. Establishment of a new family, Myriopathidae D.M. Opresko Opresko, D.M. Revision of the Antipatharia (Cnidaria: Anthozoa). Part I. Establishment of a new fami- ly, Myriopathidae. Zool. Med. Leiden 75 (17), 24.xii.2001: 343-370, figs. 1-18.— ISSN 0024-0672. Dennis M. Opresko, Life Sciences Division, Oak Ridge National Laboratory, 1060 Commerce Park, Oak Ridge, TN 37830, U.S.A. (e-mail: [email protected]). Key words: Cnidaria; Anthozoa: Antipatharia; Myriopathidae fam. nov.; Myriopathes gen. nov.; Cupressopathes gen. nov.; Plumapathes gen. nov.; Antipathella Brook; Tanacetipathes Opresko. A new family of antipatharian corals, Myriopathidae (Cnidaria: Anthozoa: Antipatharia), is estab- lished for Antipathes myriophylla Pallas and related species. The family is characterized by polyps 0.5 to 1.0 mm in transverse diameter; short tentacles with a rounded tip; acute, conical to blade-like spines up to 0.3 mm tall on the smallest branchlets or pinnules; and cylindrical, simple, forked or antler-like spines on the larger branches and stem. Genera are differentiated on the basis of morphological fea- tures of the corallum. Myriopathes gen. nov., type species Antipathes myriophylla Pallas, has two rows of primary pinnules, and uniserially arranged secondary pinnules. Tanacetipathes Opresko, type species T. tanacetum (Pourtalès), has bottle-brush pinnulation with four to six rows of primary pinnules and one or more orders of uniserial (sometimes biserial) subpinnules. Cupressopathes gen. nov., type species Gorgonia abies Linnaeus, has bottle-brush pinnulation with four very irregular, or quasi-spiral rows of primary pinnules and uniserial, bilateral, or irregularly arranged higher order pinnules.