María Victoria Cerecetto González Curriculum Vitae

Personal information:

Date of Birth: 06 September 1990. Place of Birth: Montevideo, Uruguay. Home Address: Durazno 1530 ap. 1002-PC 11200-Montevideo. Cellphone: (+598) 98 56 45 08 E-mail: [email protected]

Education:

2014-Present: M.Sc. in Biotechnology, Faculty of Sciences, University of the Republic (UdelaR), Uruguay. Thesis: “Microorganisms that act on the phytoavailability of phosphorus: detection, mechanisms involved and potencial use as biofertilizers”. Supervisor: Dr. Elena Beyhaut. Place: Laboratory of Microbiolgy of Soils, National Institute for Agricultural Research (INIA). Courses taken within the M.Sc. In Biotechnology:  Biology of Soils: theoretical and practical aspects for the study of the edaphic communities. National University of Quilmes, Quilmes, Buenos Aires, Argentine.  Entomopathogenic fungi for the biological control of plagues. Faculty of Agronomy, UdelaR, Uruguay.  Environmental and agricultural microbiology. Faculty of Chemistry, UdelaR, Uruguay.  Soil-plant relationships. Faculty of Agronomy, UdelaR, Uruguay.  Environmental biotechnology. Federal University of Goiás, Goiânia, Goiás, Brazil.  Plant growth promoting bacteria and their interaction with the plants. Institute of Biological Research Clemente Estable (IIBCE), Uruguay.  Statistics I. Faculty of Agronomy, UdelaR, Uruguay.  Metagenomic bioprospection techniques. IIBCE, Uruguay.  Writing of scientific articles, INIA, Uruguay. Currently, i finished the experimental work and i'm writing the manuscript of the thesis and an article. Academic average: 11,20 (over 12).

2009-2014: B.Sc. in Biochemistry, Faculty of Sciences, University of the Republic, Uruguay. Bachelor thesis: “Poliphasic approach to the characterization of cyanobacterias with heterocysts isolated from rice soils”. Supervisors: M.Sc. Pilar Irisarri and M.Sc. Germán Pérez. Place: Laboratory of Microbiology, Plant Biology Department, Faculty of Agronomy, UdelaR, Uruguay. Academic average: 7,45 (over 12).

Computer Skills: Basic R Advanced Infostat, Windows

Languages:

Spanish Mothertongue English Level First Certificate, University of Cambridge. Reading Excellent Oral comprehesion Very good Oral expression Good Written expression Good French Reading Very good Oral comprehesion Good Oral expression Good Written expression Good Portuguese Reading Good Oral comprehesion Good Oral expression Regular

Research activities:

2017-Present: Research project: “Basis for the development of a microbial biofertilizer acting on phytoavailability of phosphorus”. P.I.: M.Sc. Silvia Garaycochea. Place: Biotechnology Unit and Laboratory of Microbiolgy of Soils, INIA, Uruguay. Funding: National Agency of Research and Innovation (ANII)-María Viñas-Uruguay. Activities: Co-responsable of the extraction of RNA from a phytate-mineralizing rhizobia, the subsequent analysis of the transcriptome and the obtention of the recombinant phytase enzime; and responsable of the plant bioassays with the recombinant enzime obtained.

2017: Training Internship: “Symbiotic nitrogen fixation and phosphorus use efficiency in Bradyrhizobium elkanii –soybean combinations in hydroaeroponic culture”. Supervisor: Dr. Jean-Jacques Drevon. Place: UMR Ecologie Fonctionnelle & Biogéochimie des Sols et Agroécoystèmes, INRA-IRD-CIRAD- SupAgro, Montpellier, France. Duration: 7 months. Funding: ANII-Mobility Scolarship-Uruguay. Activities: Plant bioassays in hydroaeroponic culture, RNA extraction, retrotranscription and PCR of rhizobial phytases, in situ RT-PCR of rhizobial phytase transcripts in sections of soybean nodules, nodular and bacterial phytase activit.

2016: Training internship: in the frame of the research project “Basis for the development of a microbial biofertilizer acting on phytoavailability of phosphorus”. Supervisors: Dr. Carlos Robello, M.Sc. Florencia Díaz Viraqué and M.Sc. Silvia Garaycochea. Place: Unity of Molecular Biology of the Institut Pasteur de Montevideo, Uruguay. Duration: 3 months. Activities: Bioinformatic research of putative phytase genes in the genome of Rhizobium tropici, cloned and expression of these genes in E. coli, and subsequently mesure of the phytase activity.

2015-2017: Research project: “Development of inoculants for the mobilization of phosphorus as an input in agricultural production”. P.I.: Dr. Nora Altier. Place: Bio-input platform, INIA, Uruguay. Funding: ANII-Alliances for the innovation-Uruguay. Activities: Supervision of the assays of phosphorus mineralization activity and phytase activity.

2014-2017: M.Sc. Thesis: “Microorganisms that act on the phytoavailability of phosphorus: detection, mechanisms involved and potencial use as biofertilizers”. Supervisor: Dr. Elena Beyhaut. Place: Laboratory of Microbiolgy of Soils, INIA, Uruguay. Funding: INIA and ANII-M.Sc. Fellow-Uruguay Activities: Phosphorus solubilization plate assays, phytate mineralization liquid assays, phytase activity, plant growth promotion bioassays.

2014-2016: Research project: “Identification of microorganisms and genes associated with the phyto-availability of phosphorus”. P.I.: Dr. Nora Altier and Dr. Elena Beyhaut. Place: Laboratory of Microbiolgy of Soils, INIA, Uruguay. Funding: INIA-Uruguay. Activities: Phosphorus solubilization plate assays, phytate mineralization liquid assays, phytase activity, plant growth promotion bioassays.

2012-2013: Research project: “Nitrous oxide emission in rice cultures: evaluation of some management variables on microorganisms involved in the nitrogen cycle”. P.I.: M.Sc. Pilar Irisarri. Place: Laboratory of Microbiology, Plant Biology Department, Faculty of Agronomy, UdelaR, Uruguay. Funding: Sectoral Commission of Scientific Research (CSIC)-Uruguay.

Activities: Culture of rice in greenhouse, greenhouse gas sampling, quantification of N20 by GC, quantification of aminoacids and organic acids of rice rooth exudates by HPLC, quantification of soluble carbon in rice rooth exudates, mesure of potential of soil mineralization, quantification of concentration of ammonium and nitrate in soils and counting of nitrifying and denitrifying bacterias in soil.

2012-2013: Bachelor thesis: “Poliphasic approach to the characterization of cyanobacterias with heterocysts isolated from rice soils”. Supervisors: M.Sc. Pilar Irisarri and M.Sc. Germán Pérez. Place: Laboratory of Microbiology, Plant Biology Department, Faculty of Agronomy, UdelaR, Uruguay. Funding: ANII-Undergaduate Research Initiation Fellow-Uruguay. Activities: Characterization of cyanobacterial isolates by morphological analysis and molecular analysis (16S and hetR genes), mesure of enzimatic activities like nitrate reductase, glutamine synthetase and nitrogenase, quantification of pigments like chlorophyll a, carotenoids and phycobilins, mesure of the O2 photosynthetic production rate. 2011: Research project: “Evaluation of cyanobacteria blooms in a tourist area of Río Negro (Paso de los Toros, Tacuarembó)”. P.I.: B.Sc. Victoria Cerecetto, B.Sc. Agustina Berchesi and M.Sc. Florencia Díaz Viraque. Supervisor: Dr. Luis Aubriot. Place: Limnology Section, Faculty of Sciences, UdelaR, Uruguay. Funding: CSIC-Program to Support Student Research-Uruguay. Activities: Water sampling, quantification of pigments like chlorophyll a and phycobilins, quantification of biomass, quantification of dissolved nutrients like phosphate, nitrate, nitrite and ammonium, and quantification of total nutrients like phosphorus and nitrogen.

2011: Research project: “Use of ruminal content in reductions of aromatic N-oxides”. P.I.: Dr. Mercedes González. Place: Laboratory of Organic Chemistry, Medical Chemistry Group, Faculty of Sciences, Uruguay. Activities: Extraction and handling of a dairy cow's rumen, study of the bioreduction of aromatic amine N-oxide compounds using rumen as a reducing agent, following this process by thin layer chromatography and later, isolating and purifying the final organic compounds.

Publications:

2013 Cerecetto, V.; Díaz-Viraqué, F.; Irazoqui, I.; Rodríguez, A.; Cajaraville, C.; Repetto, J.; Lavaggii, M. L.; González, M. & Cerecetto, H. Anaerobic Biotransformation of N-oxide Containing Aromatic Heterocycles by Bovine Ruminal Fluid, Revista Virtual de Química. DOI: 10.5935/1984-6835.20130082.

2009 Scaglia, F.; Bello, D.; Cerecetto, V. & Fernández, D. La Erosión como Riesgo Geológico. Revista SUG. 15: 68-77.

Conference Presentations:

Oral Presentations:

2017 Microorganisms for Agriculture Symposium, Uruguay. Cerecetto, V.; Amenc, L.; Trives, C.; Teffahi, M.; Rojas, L.; Altier, N.; Beyhaut, E. & Drevon, J.-J. “Rhizobial phytases and efficiency in the use of phosphorus in the Bradyrhizobium elkanii-soybean symbiosis in hydroaeroponic culture”

2016 3rd Latin American workshop on plant growth promoting rhizobacteria, Chile. Cerecetto, V.; Altier, N. & Beyhaut, E. “Evaluation of phosphate solubilizing and mineralizing bacteria able to enhance plant growth”

2015 IX Agrobiotechnology Conference, Uruguay. Barlocco, C.; Cerecetto, V.; Mattos, N.; Mortalena, M.; Mayans, M.; Beyhaut, E. & Altier, N. “Characterization of the National Collection of Rhizobian Strains: Multifunctionality” 2013 X National Meeting of Microbiologists, Uruguay. Illarze, G.; Cerecetto, V.; Gutiérrez, P. &

Irisarri, P.“Micro-organisms producers of N2O in rice soils: effect of soil type and water management”

2012 Students Research Exchange Forum (CSIC), Uruguay. Cerecetto, V.; Díaz-Viraqué, F.; Berchesi, A. & Aubriot, L. “Evaluation of cyanobacteria blooms in a tourist area of Río Negro (Paso de los Toros, Tacuarembó)”

Poster Presentations:

2011 7th Veterinary Technical Conference, Uruguay. Cerecetto, V.; Díaz-Viraqué, F.; Irazoqui, I.; Rodríguez, A.; Bonifacino, C.; Lavaggi, M. L.; Álvarez, G.; Cajarville, C.; Repetto, J. L.; Cerecetto, H. & González, M. “Use of bovine ruminal liquid as bioreactor”

2012 XIV Seminars of the Uruguayan Society of Biosciences, Uruguay. Cerecetto, M. V.; Pérez, G. & Irisarri, P. “Characterization of cyanobacterias with herecocysts isolated from soil of rice”

2013 X National Meeting of Microbiologists, Uruguay. Cerecetto, M. V.; Pérez, G. & Irisarri, P. “Cyanobacterias with heterocysts: characterization of natives strains for use as rice inoculants”

2014 I National Meeting of Young Microbiologists, Uruguay. Cerecetto, M. V.; Pérez, G. & Irisarri, P. “Poliphasic approach to the characterization of cyanobacterias with heterocysts isolated from rice soils”

2015 XI National Meeting of Microbiologists, Uruguay. Cerecetto, V. & Beyhaut, E. “Microorganisms that act on the phitodisponibility of the phosphorus and development of biofertilizers”

2015 10th International Plant Growht Promoting Rhizobacteria Workshop, Belgium. Cerecetto, V.; Barlocco, C.; Vaz, P. & Beyhaut, E. “Use of phytate-mineralizing microorganisms to enhance plant-available P”

2015 IX Agrobiotechnology Conference, Uruguay. Cerecetto, V. & Beyhaut, E. “Microorganisms that act on the phitodisponibility of the phosphorus and development of biofertilizers”

2016 XXVII Latin American Reunion of Rhizobiology, Brazil. Cerecetto, V.; Abreo.; E., Beyhaut, E. & Altier, N. “Evaluation of Bacillus spp. for the mineralization and solubilization of phosphorous in soils”

2016 XXVII Latin American Reunion of Rhizobiology, Brazil. Barlocco, C.; Cerecetto, V.; Rostán, V.; Mattos, N.; Mortalena, M.; Mayans, M.; Altier, N. & Beyhaut, E. “Characterization and multifunctionality of the National Collection of Rhizobia Strains in Uruguay” 2017 20th International Congress on Nitrogen Fixation, Spain. Cerecetto, V.; Trives, C.; Amenc, L.; Beyhaut, E. & Drevon, J.-J. “Symbiotic nitrogen fixation and phosphorous use efficiency in Bradyrhizobium elkanii-soybean combinations in hydroaeroponic culture”

2017 III Iberoamerican Conference of Beneficial Plant-Microorganisms-Environment Interactions, Peru. Cerecetto, V.; Altier, N.; Drevon, J.-J. & Beyhaut, E. “Potencial use as biofertilizers of phytate-mineralizing bacteria”

Teaching:

2015: Training of laboratory techniques to a Bachelor in Agricultural and Environmental Chemistry student. Place: Laboratory of Microbiology of soils, INIA, Uruguay.

2014-2015: Professor of Chemistry of Constructive Processes in the University of Work, Uruguay (UTU).

2014: Privates lessons of Biology, Chemistry and Phisics in the Wolf Academy, Uruguay.

2013: Training of laboratory techniques to a M.Sc. in Agricultural Sciences student. Place: Laboratory of Microbiology, Plant Biology Department, Faculty of Agronomy, UdelaR, Uruguay.

2012: Teaching assistant in the course of Microbiology for Agronomist Engineers, Faculty of Agronomy, UdelaR, Uruguay.

Work Experience:

2016-2017: Research assistant, Laboratory of Microbiology of Soils, INIA, Uruguay.

2014-Present: M.Sc. student, Laboratory of Microbiology of Soils, INIA, Uruguay.

2014-2015: Scholar, Laboratory of Microbiology of Soils, INIA, Uruguay.

2014-2015: Professor, UTU, Uruguay.

2014: Teacher of private lessons, Wolf Academy, Uruguay.

2012-2013: Research assistant, Laboratory of Microbiology, Plant Biology Department, Faculty of Agronomy, UdelaR, Uruguay.

2012-2013: Honorary assistant as teaching assistant, Laboratory of Microbiology, Plant Biology Department, Faculty of Agronomy, UdelaR, Uruguay.

2012-2013: Undergraduate student, Laboratory of Microbiology, Plant Biology Department, Faculty of Agronomy, UdelaR, Uruguay.

2011-2012: Functionary, University Cinema of Uruguay.

Volunteering:

2013-2014: Tutorials of Mathematics, Physics, Chemistry and Biology to high school students in vulnerable situations. Teen Space Together, El Dorado, Canelones, Uruguay.

Awards and Fellowships:

2017 Scholarship from the biotechnology post graduate programe, Faculty of Science, UdelaR, Uruguay, to attend a the 20th International Congress on Nitrogen Fixation, in Granada, Spain. 2016 Mobility scholarship, ANII, Uruguay. 2015-2017 M.Sc. fellow, ANII, Uruguay. 2015 International Plant Nutrition Institute (IPNI) Scholar Award. 2015 Scholarship from the Brazilian Argentine Center of Biotechnology (CABBIO) to attend a the course “Environmental biotechnology”, in Goiânia, Goiás, Brazil. 2014 Scholarship from the CABBIO to attend a the course “Biology of Soils: theoretical and practical aspects for the study of the edaphic communities” in Quilmes, Buenos Aires, Argentine. 2012-2013: Undergraduate Research Initiation Fellow, ANII, Uruguay. 2011: Program to Support Student Research, CSIC, Uruguay. 2008: Second prize of the Contest "2008, International Year of the Planet Earth", Uruguayan Society of Geology.

November 19, 2017