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2021 IPSF Meeting Abstracts 2021 INTERNATIONAL POULTRY SCIENTIFIC FORUM ABSTRACTS January 25-26, 2021 ABSTRACTS 2021 Virtual International Poultry Scientific Forum January 25-26, 2021 Table of Contents ABSTRACTS POSTER SESSIONS Dendy Keynote Lecture, 1 Teaching, Pedagogy, Extension, 36 Physiology, Endocrinology and Reproduction: Broilers, 2 Physiology, Endocrinology and Reproduction: Broilers, 38 Processing and Products, 3 Physiology, Endocrinology and Reproduction: Layer or Pathology, 6 Broiler Breeders, 38 Environment, Management and Animal Well-Being: Processing and Products, 39 Environmental Impacts, 7 Environment, Management and Animal Well-Being: Environment, Management and Animal Well-Being: Stress Environmental Impacts, 41 Responses, Behavior, 9 Environment, Management and Animal Well-Being: Stress Metabolism and Nutrition: Enzymes, Feed Additives, 11 Responses, Behavior, 42 SCAD, 15 Environment, Management and Animal Well-Being: Environmental Impacts, 43 Metabolism and Nutrition: General Nutrition, 17 Environment, Management and Animal Well-Being: Stress Metabolism and Nutrition: Vitamins and Minerals, 19 Responses, Behavior, 43 Metabolism and Nutrition: Amino Acids, 21 Metabolism and Nutrition: Enzymes, Feed Additives, 45 Physiology, Endocrinology and Reproduction: Broilers, 22 Metabolism and Nutrition: General Nutrition, 51 Teaching, Pedagogy, Extension, 23 Metabolism and Nutrition: Vitamins and Minerals, 55 Processing and Products, 23 Pathology, 55 SCAD, 24 SCAD, 58 Metabolism and Nutrition: Amino Acids, 27 Metabolism and Nutrition: General Nutrition, 28 AUTHOR INDEX, 62 Metabolism and Nutrition: Vitamins and Minerals, 29 Metabolism and Nutrition: Enzymes, Feed Additives, 31 ABSTRACTS 2021 Virtual International Poultry Scientific Forum January 25-26, 2021 Dendy Keynote Lecture KEYNOTE, Impact of COVID-19 in the broiler chicken industry Ashley Peterson* National Chicken Council COVID-19 changed everything, everywhere and has impacted all of us in different ways. From masks and social distancing to home offices and virtual meetings, 2020 has certainly been a challenging year. We saw cleaners and disinfectants disappear from grocery shelves overnight. Paper products were scarce and when available, they were rationed. The food supply chain was also dramatically impacted as dining out options were limited, travel virtually came to a halt, and eating three meals a day at home became the norm. With the increase in preparing meals at home, grocery sales soared, and the supply chain struggled to keep up with demand. Amidst the ever-changing situation, the chicken industry relied on their essential frontline workers to restock grocery shelves and ensure that we all had safe and nutritious chicken. The chicken industry adjusted quickly by retrofitting production lines to help with social distancing, providing employees with PPE and temperature screenings, enhancing sanitation procedures, and many other measures to help keep employees safe while they were working to ensure that meat cases were stocked with chicken. While most of these changes were implemented in early Spring 2020, the industry has continued to provide a safe environment for essential frontline workers and to produce safe and wholesome chicken and chicken products. Though 2020 has presented many new challenges opportunities, the industry has and will continue to provide the number one protein of choice – chicken. 1 2, ABSTRACTS OF PAPERS *Author presenting paper GS Denotes Graduate Student Competition UG Denotes Undergraduate Presentation Physiology, Endocrinology and Reproduction: Broilers M1 Effect of Wooden Breast severity on myogenic regulatory = mild; 2 = severe) were collected at 25 and 43 d post-hatch (n = 5 samples factor and cytokine protein expression in 25- and 43- day-old broiler per score per age). Target proteins and total protein were quantified using chickens Joshua Flees*GS, Katherine Meloche, Jessica Starkey Auburn Image Quant TL 8.1 software. Target proteins were first normalized to to- University Department of Poultry Science tal protein in each lane and then set relative to WB score 0. Data were ana- lyzed as a one-way ANOVA and least square means were separated using Previous research aimed at elucidating the etiology of Wooden Breast SAS (V9.4) PROC GLIMMIX and PDIFF for multiple means compari- (WB) revealed increased collagen and macrophage infiltration as well sons at P < 0.05. On d 25, the expression of ATG5 tended to be lower in as altered densities of muscle satellite (stem; SC) cell populations with birds with mild and severe WB compared with normal birds (P = 0.0640). different myogenic regulatory factor (MRF) expression profiles in breast The protein expression of p62 on d 25 was decreased in severe compared muscles of severely affected broilers. The objective of the study was to to normal and mildly affected birds (P = 0.0438). Expression of LAMP2 use quantitative, multiplex, fluorescent Western blotting to determine the protein was decreased in WB-affected compared with normal at d 25 (P effect of WB severity on the protein expression of 3 MRF (myogenic dif- = 0.0026). On d 43, Beclin1 protein was more abundant in WB-affected ferentiation factor 1; MyoD, paired box 7; Pax7, and myogenin) and 3 birds compared with normal (P ≥ 0.2380). However, expression of ATG5, cytokines (interleukin-1β; IL-1β, interleukin-6; IL6, and interleukin-10; p62, LAMP2, and LC3B were unaltered by WB in breast muscle from IL-10). Muscle (Pectoralis major) samples from birds with varying WB 43-d-old broilers (P = 0.0159). Overall, expression of autophagy proteins severity scores (0 = normal; 1 = mild; 2 = severe) were collected at 25 and from birds at 25 and 43 d-of-age was altered by WB. These results sug- 43 d post-hatch (n = 5 samples per score per age). Target proteins and total gest that the degradation and recycling of cellular components may be protein were quantified using Image Quant TL 8.1 software. Target pro- disrupted in WB-affected compared with normal breast muscle. Further teins were first normalized to total protein in each lane and then set relative investigation of protein expression in various pathways is warranted to to WB score 0. Data were analyzed as a one-way ANOVA and least square better understand the etiology of WB. means were separated using SAS (V9.4) PROC GLIMMIX and PDIFF for multiple means comparisons at P < 0.05. On d 25, MyoD protein ex- Key Words: Wooden Breast, Woody Breast, autophagy, protein pression was similar (P = 0.7803) and Pax7 protein was more abundant expression, broiler chicken in WB-affected (score 1 and 2) birds compared with normal (P = 0.0025). Myogenin protein expression was greater in severe d 25 birds compared M3 Effect of dietary 25OHD3 supplementation on broiler chicken to mild (P = 0.0073). On d 43, MyoD protein abundance was decreased ileal IL-10 and IL-17 protein expression Gerardo Abascal-Ponciano*GS, in WB-affected muscle compared with normal (P = 0.0017). However, Samuel Leiva, Jessica Starkey, Charles Starkey Auburn University Poultry Pax7 and myogenin expression were unaltered by WB (P = 0.8392). At Science Department d 25, IL-6 (P = 0.0009) and IL-10 (P = 0.0063) protein abundance was decreased in WB-affected compared with normal muscle, while IL-1β Vitamin D signaling is important for intestinal homeostasis. Increased was unaffected (P = 0.1071). Yet, on d 43, expression of IL-1β, IL-6, and presence of vitamin D receptor in immune cells modulates cell phenotype IL-10 was unaltered in WB-affected birds compared with normal (P ≥ and secretion of cytokines. Cytokines regulate inflammatory responses 0.7064). In conclusion, alterations in the expression of MRF suggests that triggered by external stimuli. Interleukins are a large family of cytokines muscle SC proliferation and differentiation capacities may be impacted secreted by cells in the intestine and can modulate both pro-inflammatory in WB-affected broilers. In alignment with previous reports of increased (Interleukin 17; IL-17) and anti-inflammatory (Interleukin 10; IL-10) re- macrophage density at d 25, cytokine expression was also altered in WB- sponses. The objective of the study was to determine the impact of dietary affected birds. supplementation with 25-hydroxycholecalciferol (25OHD3) on the local immune response over time in broilers. A randomized complete block de- Key Words: Wooden Breast, myogenic regulatory factor, cytokine, sign experiment was conducted to evaluate the effect of post-hatch dietary Woody Breast, broiler chicken 25OHD3 inclusion on IL-17 and IL-10 protein expression in ileum of broiler chickens from 3 to 21 d post-hatch. On d of hatch, male chicks (n = M2 Effect of Wooden Breast severity on autophagy pathway 480) were randomly assigned to raised floor pens. The experimental corn- protein expression in 25- and 43-day-old broiler chickens Caroline soybean meal based treatments were: 1) common starter diet containing Gregg*GS, Joshua Flees, Katherine Meloche, Jessica Starkey Auburn 5,000 IU of D3 per kg of feed (D3) and 2) common starter diet containing University Department of Poultry Science 2,240 IU of D3 + 2,760 IU of 25OHD3 per kg of feed (25OHD3) fed from d 0 to 21. From d 0 to 7, birds were housed with 4 birds per pen The underlying cellular and molecular mechanisms leading to the devel- (0.05 m2 2 opment of the Wooden Breast (WB) myopathy in broilers have not yet per bird) and individually (0.21 m per bird) from d 8 to 21. On d been fully elucidated. Autophagy is necessary for the degradation and 3, 6, 9, 12, 15, 18, and 21, 12 birds from each treatment were euthanized recycling of cellular components but it has yet, to our knowledge, to be and ileum tissue samples were collected and analyzed using quantitative, studied in WB affected muscle tissue. The objective of this study was to multiplex, fluorescent Western blotting. Target proteins were quantified use quantitative, multiplex fluorescent Western blotting to determine the using Image Quant TL 8.1 and expressed relative to total protein in each effect of WB severity on the expression of 5 autophagy pathway proteins lane.
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