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PRKCQ / PKC-Theta Antibody (Aa640-690) Rabbit Polyclonal Antibody Catalog # ALS16240

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PRKCQ / PKC-Theta Antibody (Aa640-690) Rabbit Polyclonal Antibody Catalog # ALS16240 10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 PRKCQ / PKC-Theta Antibody (aa640-690) Rabbit Polyclonal Antibody Catalog # ALS16240 Specification PRKCQ / PKC-Theta Antibody (aa640-690) - Product Information Application WB, IHC Primary Accession Q04759 Reactivity Human, Mouse, Rat Host Rabbit Clonality Polyclonal Calculated MW 82kDa KDa PRKCQ / PKC-Theta Antibody (aa640-690) - Additional Information Gene ID 5588 Other Names Protein kinase C theta type, 2.7.11.13, Western blot of PKC (N670) pAb in extracts nPKC-theta, PRKCQ, PRKCT from A549 cells. Target/Specificity Human PKC Theta Reconstitution & Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Precautions PRKCQ / PKC-Theta Antibody (aa640-690) is for research use only and not for use in diagnostic or therapeutic procedures. Anti-PRKCQ / PKC-Theta antibody IHC staining of human brain, cerebellum. PRKCQ / PKC-Theta Antibody (aa640-690) - Protein Information PRKCQ / PKC-Theta Antibody (aa640-690) - Name PRKCQ Background Synonyms PRKCT Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent Function serine/threonine-protein kinase that mediates Calcium-independent, phospholipid- and non- redundant functions in T-cell receptor diacylglycerol (DAG)- dependent (TCR) signaling, including T-cells activation, serine/threonine-protein kinase that proliferation, differentiation and survival, by mediates non-redundant functions in T-cell mediating activation of multiple transcription receptor (TCR) signaling, including T-cells factors such as NF-kappa-B, JUN, NFATC1 and activation, proliferation, differentiation and NFATC2. In TCR-CD3/CD28-co-stimulated Page 1/3 10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 survival, by mediating activation of multiple T-cells, is required for the activation of transcription factors such as NF-kappa-B, NF-kappa-B and JUN, which in turn are JUN, NFATC1 and NFATC2. In essential for IL2 production, and participates to TCR-CD3/CD28-co-stimulated T-cells, is the calcium-dependent NFATC1 and NFATC2 required for the activation of NF-kappa-B transactivation. Mediates the activation of the and JUN, which in turn are essential for IL2 canonical NF-kappa-B pathway (NFKB1) by production, and participates in the direct phosphorylation of CARD11 on several calcium-dependent NFATC1 and NFATC2 serine residues, inducing CARD11 association transactivation. Mediates the activation of with lipid rafts and recruitment of the the canonical NF-kappa-B pathway (NFKB1) BCL10-MALT1 complex, which then activates by direct phosphorylation of CARD11 on IKK complex, resulting in nuclear translocation several serine residues, inducing CARD11 and activation of NFKB1. May also play an association with lipid rafts and recruitment indirect role in activation of the non- canonical of the BCL10-MALT1 complex, which then NF-kappa-B (NFKB2) pathway. In the signaling activates IKK complex, resulting in nuclear translocation and activation of NFKB1. May pathway leading to JUN activation, acts by also play an indirect role in activation of the phosphorylating the mediator STK39/SPAK and non-canonical NF- kappa-B (NFKB2) may not act through MAP kinases signaling. pathway. In the signaling pathway leading Plays a critical role in TCR/CD28-induced to JUN activation, acts by phosphorylating NFATC1 and NFATC2 transactivation by the mediator STK39/SPAK and may not act participating in the regulation of reduced through MAP kinases signaling. Plays a inositol 1,4,5-trisphosphate generation and critical role in TCR/CD28- induced NFATC1 intracellular calcium mobilization. After and NFATC2 transactivation by participating costimulation of T-cells through CD28 can in the regulation of reduced inositol phosphorylate CBLB and is required for the 1,4,5-trisphosphate generation and ubiquitination and subsequent degradation of intracellular calcium mobilization. After CBLB, which is a prerequisite for the activation costimulation of T-cells through CD28 can of TCR. During T-cells differentiation, plays an phosphorylate CBLB and is required for the important role in the development of T-helper ubiquitination and subsequent degradation 2 (Th2) cells following immune and of CBLB, which is a prerequisite for the inflammatory responses, and, in the activation of TCR. During T-cells development of inflammatory autoimmune differentiation, plays an important role in diseases, is necessary for the activation of the development of T-helper 2 (Th2) cells IL17-producing Th17 cells. May play a minor following immune and inflammatory role in Th1 response. Upon TCR stimulation, responses, and, in the development of mediates T-cell protective survival signal by inflammatory autoimmune diseases, is phosphorylating BAD, thus protecting T-cells necessary for the activation of from BAD-induced apoptosis, and by IL17-producing Th17 cells. May play a minor up-regulating BCL-X(L)/BCL2L1 levels through role in Th1 response. Upon TCR stimulation, mediates T-cell protective survival signal by NF-kappa-B and JUN pathways. In platelets, phosphorylating BAD, thus protecting regulates signal transduction downstream of T-cells from BAD-induced apoptosis, and by the ITGA2B, CD36/GP4, F2R/PAR1 and up-regulating BCL-X(L)/BCL2L1 levels F2RL3/PAR4 receptors, playing a positive role through NF- kappa-B and JUN pathways. In in 'outside-in' signaling and granule secretion platelets, regulates signal transduction signal transduction. May relay signals from the downstream of the ITGA2B, CD36/GP4, activated ITGA2B receptor by regulating the F2R/PAR1 and F2RL3/PAR4 receptors, uncoupling of WASP and WIPF1, thereby playing a positive role in 'outside-in' permitting the regulation of actin filament signaling and granule secretion signal nucleation and branching activity of the Arp2/3 transduction. May relay signals from the complex. May mediate inhibitory effects of free activated ITGA2B receptor by regulating the fatty acids on insulin signaling by uncoupling of WASP and WIPF1, thereby phosphorylating IRS1, which in turn blocks permitting the regulation of actin filament IRS1 tyrosine phosphorylation and downstream nucleation and branching activity of the activation of the PI3K/AKT pathway. Arp2/3 complex. May mediate inhibitory Phosphorylates MSN (moesin) in the presence effects of free fatty acids on insulin of phosphatidylglycerol or phosphatidylinositol. Page 2/3 10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 signaling by phosphorylating IRS1, which in Phosphorylates PDPK1 at 'Ser-504' and turn blocks IRS1 tyrosine phosphorylation 'Ser-532' and negatively regulates its ability to and downstream activation of the PI3K/AKT phosphorylate PKB/AKT1. pathway. Phosphorylates MSN (moesin) in the presence of phosphatidylglycerol or PRKCQ / PKC-Theta Antibody (aa640-690) - phosphatidylinositol. Phosphorylates PDPK1 References at 'Ser-504' and 'Ser-532' and negatively regulates its ability to phosphorylate Chang J.D.,et al.J. Biol. Chem. PKB/AKT1. Phosphorylates CCDC88A/GIV 268:14208-14214(1993). and inhibits its guanine nucleotide Chang J.D.,et al.J. Biol. Chem. exchange factor activity (PubMed:<a href=" 269:31322-31322(1994). http://www.uniprot.org/citations/23509302" Baier G.,et al.J. Biol. Chem. target="_blank">23509302</a>). 268:4997-5004(1993). Li H.,et al.Submitted (JUL-2004) to the Cellular Location EMBL/GenBank/DDBJ databases. Cytoplasm. Cell membrane; Peripheral membrane protein. Note=In resting T-cells, Ota T.,et al.Nat. Genet. 36:40-45(2004). mostly localized in cytoplasm. In response to TCR stimulation, associates with lipid rafts and then localizes in the immunological synapse Tissue Location Expressed in skeletal muscle, T-cells, megakaryoblastic cells and platelets. Volume 50 µl PRKCQ / PKC-Theta Antibody (aa640-690) - Protocols Provided below are standard protocols that you may find useful for product applications. • Western Blot • Blocking Peptides • Dot Blot • Immunohistochemistry • Immunofluorescence • Immunoprecipitation • Flow Cytomety • Cell Culture Page 3/3 Powered by TCPDF (www.tcpdf.org).
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