DOCSLIB.ORG

A New Series of Hydrazide-Hydrazones Based 2-Oxonicotinonitriles As Antimicrobial Agents: Design, Synthesis and Antimicrobial Evaluation

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
A New Series of Hydrazide-Hydrazones Based 2-Oxonicotinonitriles As Antimicrobial Agents: Design, Synthesis and Antimicrobial Evaluation A new series of hydrazide-hydrazones based 2-oxonicotinonitriles as antimicrobial agents: Design, synthesis and antimicrobial evaluation H.A. El-Sayed1*, A. H. Moustafa1#, M. Abd El-Moneim3, H. M. Awad2 and A. Esmat3 1Department of Chemistry, Faculty of Science, Zagazig University, Zagazig, Egypt. 2Department of Tanning Materials and Leather Technology, National Research Centre, Dokki 12622, Cairo, Egypt. 3Department of Chemistry, Faculty of Science, Port Said University, Port Said, Egypt Una nueva serie de 2-oxonicotinonitrilos a base de hidrazida-hidrazonas como agentes antimicrobianos: Diseño, síntesis y evaluación antimicrobiana Una nova sèrie de 2-oxonicotinonitrilos a base de hidrazida-hidrazonas com agents antimicrobians: Disseny, síntesi i avaluació antimicrobiana RECEIVED: 6 JANUARY 2018; REVISED: 15 MARCH 2018; ACCEPTED: 5 APRIL 2018 lizados a excepción de los compuestos 4b, 5c, 5d y SUMMARY 6b que mostraban una actividad moderada hacia el B. subtilus. Regiospecific alkylation of 2-oxonicotinonitriles 1a,b with ethyl bromoacetate followed by hydrozinol- Palabras clave: 2-Oxonicotinonitrilo; alquilación; ysis afforded acetic acid hydrazides3a,b . The latter hidrazida ácida; hidrazida-hidrazona; antimicrobia- compounds were condensed with a variety of carbonyl na. containing compounds to give the target compounds of hydrazones 4a,b, 5a,b, 6a-h and 7a,b. The antimi- RESUM crobial activity of the new synthesized compounds do not showed activity against tested micro-organisms Alquilació regioespecífica de 2-oxonicotinonitrilos except compounds 4b, 5c, 5d and 6b showed moder- 1a,b amb bromoacetato de etilo seguida de la hidra- ate activity towards B. subtilus zinolisis permès per les hidrazides d’àcid acètic 3a,b. Aquests últims compostos es condensaven amb una Keywords: 2-Oxonicotinonitrile; alkylation; acid varietat de compostos que tenen carbonil, per donar hydrazide; hydrazide-hydrazone; antimicrobial. lloc a compostos “target” a base de hidrazones 4a,b, 5a,b, 6a-h y 7a,b. La activitat antimicrobiana dels nous compostos sintetitzats no va aparèixer contra els RESUMEN microorganismes avaluats a excepció dels compostos 4b, 5c, 5d i 6b que mostraven una activitat moderada Alquilación regioespecífica de 2-oxonicotinonitrilos versus el B. subtilus 1a,b con bromoacetato de etilo seguida de la hidrazi- nolisis permitida por las hidrazidas de ácido acético Paraules clau: 2-Oxonicotinonitrilo; alquilació; hi- 3a,b. Estos últimos compuestos se condensaban con drazida àcida; hidrazida-hidrazona; antimicrobiana. una variedad de compuestos que contienen carbonilo para dar lugar a los compuestos destinatarios a base de hidrazonas 4a,b, 5a,b, 6a-h y 7a,b. La actividad antimicrobiana de los nuevos compuestos sintetiza- *Corresponding authors:*[email protected]; ah_ dos no era evidente contra los microorganismos ana- [email protected] OCTOBER- DECEMBER 2018 | 305 1. INTRODUCTION 2-Oxonicotinonitrile (2-ONN) and its derived compounds are an important class of nitrogen containing heterocyclic systems, which have been undergo to extensive study in the recent years due to their chemical and pharmaceutical interest. Nic- otinonitriles have been reported to have antimi- crobial1,2, analgesic, anti-inflammatory, antipyret- ic3,4 anticancer2, cardiotonic5, antihypertensive6,7 properities. Another interested biological scaffold is given by hydrazide-hydrazone (CO-NHN=C) system, which are used to improve the biological activity of a main compound. For example, many compounds containing hydrazide-hydrazone moi- ety posses a wide range of remarkable biological properties such as antimalarial8, antituberculo- sis9, anti-HIV10, antimicrobial11,12, anti-inflamma- tory13 and anticonvulsant14,15. In recent years, our research efforts focused on the synthesis of func- tionalized pyridines for biological evaluation1,2,16,17. Herein, we linked hydrazide-hydrazone moiety to nicotinonitrile ring and investigate the antimicro- bial activity of such products. 2. RESULTS AND DISCUSSION 2.1. Chemistry 4-Aryl-6-naphth-2-yl-2-oxo-1,2-dihydronicoti- nonitriles 1a,b were selected as starting synthon for this research work. Compounds 1a,b have been synthesized as in literature2,16 via one pot multi- component condensation of 2-acetyl naphthalene, aromatic aldehyde (namely, 4-methyl benzalde- Scheme 3 illustrates the synthetic routes of tar- hyde and 3-methyl thiophene-2-carboxaldehyde), get compounds 4-7. Where, condensation of hy- ethyl cyanoacetate and ammonium acetate in re- drazides 3a,b with benzaldehyde, acetophenone, fluxing ethanol (Scheme 1). The spectroscopic data 4-nitroacetophene, 4-chloroacetophenone, 4-bro- and microanalysis were agreed with the assigned moacetophenone and ethyl acetoacetate afforded structure. Base mediate alkylation of compounds hydrazones 4a,b, 5a-h and 6a,b, respectively, in 1a,b produced compounds 2a,b (Scheme 2), which good yield (58-67%). The spectroscopic data and identified from IR bands as N-alkylated nicotino- microanalysis were agreed with the assigned struc- nitriles. Treatment of 2a,b with ethanolic solution ture of these compounds. The 1H NMR of com- of hydrazine hydrate under refluxing tempera- pound 5h illustrated two singlets at 2.31 and 2.35 ture afforded pyridin-1-yl acetohydrazides 3a,b ppm for 2CH3, in addition to aromatic and NH (Scheme 2). 1H NMR signals showed presence of protons signals in their regions. IR Bands of com- acetohydrazide moiety as three singlts at 4.45, 5.06 pound 6a indicated the ester-carbonyl and amide -1 1 and 9.53 ppm corresponding to NH2, CH2 and NH groups at 1747 and 1664 cm , respectively. Its H protons, respectively. NMR data confirmed that the reaction tookplace at ketonic carbonyl forming the target hydrazone, where ethoxy signals are present at 1.21 and 4.20 ppm as triplet and quartet. Another evidence for this explanation is 13C NMR data, where the ethoxy and ester-carbonyl carbons appeared at 14.20 and 64.45 and 168.4 ppm, respectively, in additon, no signal for ketonic-carbonyl carbon is present. Finally, condensation of 3a,b with acetyl acetone gave the unexpected pyrazole derivatives 7a,b via interamoleculare cyclization reaction. 1H NMR and IR data confirmed the formation of pyrazol ring. (c.f. the experimental part) (Scheme 3). 306 | AFINIDAD LXXV, 584 2.2. Antimicrobial activity at room temperature, the formed precipitate was filtered off, washed with ethanol, dried and crystal- The antimicrobial activity of new compounds were lized from methanol / acetic acid (1:2) ratio. investigated using the agar well diffusion method as 6-(Naphthalen-2-yl)-2-oxo-4-(p-tolyl)-1,2-di- modified from18, compared with Cefotaxime as con- hydro-pyridine-3-carbonitrile (1a) trol. For antifungal, Nystatin was used as standaed Yellow powder; yield 39%; m. p. 303-305 oC. IR drug. It is clearly observed that, from the obtained (KBr): 3455 cm-1 (NH), 2219 cm-1 (C=N) and 1682 -1 1 data in Table 1, all the tested compounds donot cm (C=O, amide). H NMR (DMSO-d6): δ = 2.41 showed significant antibacterial and antifungal ac- (s, 3H, CH3), 6.96 (s, 1H, pyridone H-5), 7.39 (d, 2H, tivities against the mention microorganisms, except J = 7.6 Hz, Ar-H), 7.63 (m, 4H, A-H), 8.01 (m, 4H, compounds 4b, 5c,5d and 6b showed moderate anti- A-H), 8.55 (s, 1H, Ar-H), 12.64 (br, 1H, NH). 13C microbial activity towrds B. subtilus NMR (DMSO-d6): δ = 20.94 (CH3), 97.99, 106.5, 116.6, 124.3, 127.0, 127.6, 127.9, 128.1, 128.2, 128.5, Table 1: Antimicrobial activities of some new synthesized 128.8, 129.3, 132.4,133.2, 133.8, 140.4, 151.2, 159.6, compounds (Inhibition zones mm). Diameter (mm) of 162.1 and 172.0, (C=N, Ar-C and C=O). Anal. Calcd inhibition zones against the corresponding standard for C23H16N2O (336.39): C, 82.12; H, 4.79; N, 8.33. microbial strains Found: C, 82.07; H, 4.82; N, 8.29. 4-(3-Methylthien-2-yl)-6-(naphthalen-2-yl)- Copd. No. Gm (+ve) bacteria Gm (–ve) bacteria Fungi 2-oxo-1,2-dihydropyridine-3-carbonitrile (1b) B. subtilus B. cereus P. aeruginosa E. coli Aspergillus niger Yellow powder; yield 33.5%; m. p. 283-285 oC. IR -1 -1 2a 17 19 24 18 - (KBr): 3442 cm (NH), 2217 cm (C=N) and 1640 2b 15 22 23 18 - cm-1 (C=O, amide). 1H NMR (DMSO-d ): δ = 2.32 (s, 4a 19 30 14 22 - 6 4b 33 15 16 21 16 3H, CH3), 6.95 (s, 1H, pyridone H-5), 7.13 (d, 1H, J = 5a 23 21 23 18 - 4.80 Hz, thiophene), 7.63 (m, 2H, Ar-H), 7.79 (d, 1H, 5b 22 14 12 24 - 5c 46 18 22 19 19 J = 4.80 Hz, thiophene), 8.03 (m, 4H, Ar-H), 8.53 5d 35 17 20 17 - (s, 1H, Ar-H), 12.92 (s, 1H, NH), 13C NMR (DM- 5e 25 20 21 21 20 5f 27 21 20 25 - SO-d6): δ = 15.20 (CH3), 116.2, 124.2, 127.0.127.6, 5g 30 24 21 18 - 127.9, 128.1, 128.2, 128.5, 128.9, 131.0, 131.1, 132.3, 5h 18 21 16 23 - 6a 28 28 19 24 - 133.8, 138.1, 160.2 (C=N, Ar-C and C=O). Anal. 6b 34 28 30 31 15 Calcd for C21H14N2OS (342.41): C, 73.66; H, 4.12; N, Cefotaxime 32 28 32 34 - 8.18. Found: C, 73.72; H, 4.16; N, 8.21. Nystatin - - - - 20 DMSO - - - - - General procedure for synthesis of compounds 2a,b A mixture of pyridin-2-(1H)-one-3-carbonitriles 4. EXPERIMENTAL 1a,b (10 mmol) and (11 mmol) potassium carbonate or potassium hydroxide was stirred in dry DMF (20 General mL) for 1h, followed by the addition of ethyl bro- moacetate (10 mmol), the reaction mixture was The elemental analyses were obtained on a Per- stirred at room temperature for 24 h., then poured kin Elmer 240.
Load more

Recommended publications
  • Synthesis of Hydrazide-Hydrazone Derivatives and Their Evaluation of Antidepressant, Sedative and Analgesic Agents R
    R M Mohareb et al, /J. Pharm. Sci. & Res. Vol.2(4), 2010, 185-196 Synthesis of hydrazide-hydrazone derivatives and their evaluation of antidepressant, sedative and analgesic agents 1,2 1 2 3 R. M. Mohareb , K. A. El-Sharkawy , M. M. Hussein and H. M. El-Sehrawi 1Faculty of Pharmacy, Organic Chemistry Department, October University for Modern Sciences and Arts (MSA) – El-Wahat Road – 6 October City – Egypt. 2Department of Chemistry, Faculty of Science, Cairo University, Giza, A. R. Egypt. 3Faculty of Pharmacy (Girls), Pharmaceutical Chemistry Department, Al-Azhar University, Nasr City, Cairo, A.R. Egypt. Abstract: The reaction of cyanoacetylhydrazine (1) with -bromo(4-methoxyacetophenone) (2) gave the hydrazide- hydrazone derivative 3. Compound 3 reacted with either potassium cyanide or potassium thiocyanide to give the cyanide or thiocyanide derivatives 4a or 4b respectively. The reaction of compound 3 with either hydrazine hydrate or phenylhydrazine gave the hydrazine derivatives 6a or 6b respectively. The latter compounds underwent a series of heterocyclization when react with different reagents to give 1,3,4-triazine and pyridine derivatives. The antidepressant, sedative and analgesic activities of the newly synthesized products were evaluated. Keywords: Antidepressant. hydrazide-hydrazone. pyridine. sedative. 1,3,4-triazine, Introduction: Micro Analytical Data Unit at Cairo We report here the synthesis of a series of University, Giza, Egypt. hydrazide-hydrzones via the reaction of Synthetic pathways are presented in cyanoacetylhydrazine 1 with -bromo(4- Schemes 1-2 and physicochemical, methoxyacetophenone) 2. The hydrazide- spectral data for the newly synthesized hydrazones have been demonstrated to compounds are given in Tables 1 and 2.
    [Show full text]
  • The. Reactions Op Semicarbazones, Thiosbmicarbazonbs
    THE. REACTIONS OP SEMICARBAZONES, THIOSBMICARBAZONBS AMD RELATED COMPOUNDS, IMCLTJDIMG THE ACTION OF AMINES ON AMINOCARBOCARBAZONES. A THESIS PRESENTED BY JOHN MCLEAN B.So. IN FULFILMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY OF THE UNIVERSITY OF GLASGOW. / MAY *936. THE ROYAL TECHNICAL COLLEGE, GLASGOW. ProQuest Number: 13905234 All rights reserved INFORMATION TO ALL USERS The quality of this reproduction is dependent upon the quality of the copy submitted. In the unlikely event that the author did not send a com plete manuscript and there are missing pages, these will be noted. Also, if material had to be removed, a note will indicate the deletion. uest ProQuest 13905234 Published by ProQuest LLC(2019). Copyright of the Dissertation is held by the Author. All rights reserved. This work is protected against unauthorized copying under Title 17, United States C ode Microform Edition © ProQuest LLC. ProQuest LLC. 789 East Eisenhower Parkway P.O. Box 1346 Ann Arbor, Ml 48106- 1346 This research was carried out in the Royal Technical College, Glasgow, under the supervision of Professor F.J. Wilson, whose helpful advice was greatly appreciated by the author. CONTENTS. Page General Introduction, PART 1. The Action of Amines on Amino Carhocarbazones. Introduction... ..... ,..................... 4 Benzylamine......... Theoretical.............. 11 ......... Experimental............. 15 Aniline............. Theoretical............ 21 ............. Experimental............ 22 B-Naphthylamine..... Theoretical............... 27
    [Show full text]
  • Design and Biological Evaluation of Biphenyl-4-Carboxylic Acid Hydrazide-Hydrazone for Antimicrobial Activity
    Acta Poloniae Pharmaceutica ñ Drug Research, Vol. 67 No. 3 pp. 255ñ259, 2010 ISSN 0001-6837 Polish Pharmaceutical Society DESIGN AND BIOLOGICAL EVALUATION OF BIPHENYL-4-CARBOXYLIC ACID HYDRAZIDE-HYDRAZONE FOR ANTIMICROBIAL ACTIVITY AAKASH DEEP1*, SANDEEP JAIN2, PRABODH CHANDER SHARMA3 PRABHAKAR VERMA4, MAHESH KUMAR4, and CHANDER PARKASH DORA1 1Department of Pharmaceutical Sciences, G.V.M. College of Pharmacy, Sonepat-131001, India 2Department of Pharm. Sciences, Guru Jambheshwar University of Science and Technology, Hisar-125001, India 3Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra-136119, India 4Institute of Pharmaceutical Sciences, Maharishi Dayanand University, Rohtak-124001, India Abstract: Seven biphenyl-4-carboxylic acid hydrazide-hydrazones have been synthesized. These hydrazone derivatives were characterized by CHN analysis, IR, and 1H NMR spectral data. All the compounds were eval- uated for their in vitro antimicrobial activity against two Gram negative strains (Escherichia coli and Pseudomonas aeruginosa) and two Gram positive strains (Bacillus subtilis and Staphylococcus aureus) and fun- gal strain Candida albicans and Aspergillus niger. All newly synthesized compounds exhibited promising results. Keywords: synthesis, hydrazide-hydrazones, antimicrobial activity Development of novel chemotherapeutic EXPERIMENTAL agents is an important and challenging task for the medicinal chemists and many research programs are Melting points were determined in open capil- directed towards the design and synthesis of new lary tubes and are uncorrected. Infra-red spectra drugs for their chemotherapeutic usage. Hydrazone were recorded on Perkin Elmer Spectrum RXI FTIR compounds constitute an important class for new spectrophotomer in KBr phase. 1H NMR spectra drug development in order to discover an effective were run on BRUKER spectrometer (300 MHz) compound against multidrug resistant microbial using TMS as an internal standard.
    [Show full text]
  • Toxicological Profile for Hydrazines. US Department Of
    TOXICOLOGICAL PROFILE FOR HYDRAZINES U.S. DEPARTMENT OF HEALTH AND HUMAN SERVICES Public Health Service Agency for Toxic Substances and Disease Registry September 1997 HYDRAZINES ii DISCLAIMER The use of company or product name(s) is for identification only and does not imply endorsement by the Agency for Toxic Substances and Disease Registry. HYDRAZINES iii UPDATE STATEMENT Toxicological profiles are revised and republished as necessary, but no less than once every three years. For information regarding the update status of previously released profiles, contact ATSDR at: Agency for Toxic Substances and Disease Registry Division of Toxicology/Toxicology Information Branch 1600 Clifton Road NE, E-29 Atlanta, Georgia 30333 HYDRAZINES vii CONTRIBUTORS CHEMICAL MANAGER(S)/AUTHOR(S): Gangadhar Choudhary, Ph.D. ATSDR, Division of Toxicology, Atlanta, GA Hugh IIansen, Ph.D. ATSDR, Division of Toxicology, Atlanta, GA Steve Donkin, Ph.D. Sciences International, Inc., Alexandria, VA Mr. Christopher Kirman Life Systems, Inc., Cleveland, OH THE PROFILE HAS UNDERGONE THE FOLLOWING ATSDR INTERNAL REVIEWS: 1 . Green Border Review. Green Border review assures the consistency with ATSDR policy. 2 . Health Effects Review. The Health Effects Review Committee examines the health effects chapter of each profile for consistency and accuracy in interpreting health effects and classifying end points. 3. Minimal Risk Level Review. The Minimal Risk Level Workgroup considers issues relevant to substance-specific minimal risk levels (MRLs), reviews the health effects database of each profile, and makes recommendations for derivation of MRLs. HYDRAZINES ix PEER REVIEW A peer review panel was assembled for hydrazines. The panel consisted of the following members: 1. Dr.
    [Show full text]
  • Synthesis, Characterization of New Hydrazone Derivatives Containing Heterocyclic Meioty Ahmed A
    Saheeb & Damdoom (2020): Synthesis and characterization of new hydrazine compound Oct 2020 Vol. 23 Issue 16 Synthesis, Characterization of new Hydrazone derivatives containing heterocyclic meioty Ahmed A. Saheeb1 and Wasan k.Damdoom2 1Col. of Agric. , University of Summer , Iraq. 2Col. of pharmacy, National of Science and Technology, Thi-Qar, Iraq. *Corresponding author: [email protected], [email protected] ( Damdoom) Abstract The present work includes synthesis and characterization of new hydrazine, compound (F1) derived from ethyl benzoate. Firstly, benzoate hydrazide [A1] has been synthesized from the condensation of ethyl benzoate with hydrazine. Then the benzoate hydrazide was reacted with phenylisothiocynate to prepare [B1]. Cyclization reaction of product [B1] with sodium hydroxide produced terazole derivative [C1].On the other hand, alkylation reaction of compound [C1] with chloroethylacetate in the presence of Sodium acetatetrihydrate as a catalyst resulted [D]. The acid hydrazide derivative [E1] has been synthesized by the reaction of compound [D1] with hydrazine hydrate. Finally, hydrazone derived [F1] was synthesized by the condensation reaction of the acid hydrazide [E1] with isatin. The synthesized compound was characterized by, FT-IR, 1H-NMR, 13C-NMR and Mass spectra. Compound (F2) derived from Methyl-4-hydroxybenzoate react with ethylchloroacetate ton prepare (A2) this compound react with hydrazine by reflux and used ethanol as as asolvent to give (B2). The hyrazone [C2] was synthesized by the condensation of 4-(2-hydrazino-2-oxoethoxy) benzohydrazide, and add 5 drops of acetic acid. Then the mixture refluxed for 8 hours (monitored by TLC). The hydrazone was precipitated, filtered and recrystallized from ethanol) to get white solid.
    [Show full text]
  • Synthesis of 1,1-Dialkyhydrazines and Their Hydroxyl Radical Degradation in Aqueous Environments
    Western Michigan University ScholarWorks at WMU Master's Theses Graduate College 8-2012 Synthesis of 1,1-Dialkyhydrazines and their Hydroxyl Radical Degradation in Aqueous Environments Benjamin F. Strong Follow this and additional works at: https://scholarworks.wmich.edu/masters_theses Part of the Environmental Chemistry Commons Recommended Citation Strong, Benjamin F., "Synthesis of 1,1-Dialkyhydrazines and their Hydroxyl Radical Degradation in Aqueous Environments" (2012). Master's Theses. 33. https://scholarworks.wmich.edu/masters_theses/33 This Masters Thesis-Open Access is brought to you for free and open access by the Graduate College at ScholarWorks at WMU. It has been accepted for inclusion in Master's Theses by an authorized administrator of ScholarWorks at WMU. For more information, please contact [email protected]. SYNTHESIS OF 1,1-DIALKYLHYDRAZINES AND THEIR HYDROXYL RADICAL DEGRADATION IN AQUEOUS ENVIRONMENTS by Benjamin F. Strong A Thesis Submitted to the Faculty ofthe Graduate College in partial fullilment ofthe requirements for the Degree ofMaster of Science Department ofChemistry Advisor: James J. Kiddle, Ph.D. Western Michigan University Kalamazoo, Michigan August 2012 THE GRADUATE COLLEGE WESTERN MICHIGAN UNIVERSITY KALAMAZOO, MICHIGAN Date June 21, 2012 WE HEREBY APPROVE THE THESIS SUBMITTED BY Benjamin F. Strong ENTITLED Synthesis of 1,1-dialkylhydrazines and their Hydroxyl Radical Degradation in Aqueous Environments AS PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE Master of Science DEGREE OF Chemistry (Department) James J. Kiddle Thesis Committee Chair Chemistry Blht Q(m (Program) Elke Schitffers Thesis Committee Member \ Andre Venter Thesis Committee Member APPROVED Date AjQL<=k ?OiZ Dean of The Graduate College SYNTHESIS OF 1,1-DIALKYLHYDRAZINES AND THEIR HYDROXYL RADICAL DEGRADATION IN AQUEOUS ENVIRONMENTS Benjamin F.
    [Show full text]
  • Synthesis and Characterization of Some Biologically Important 1-Isopropyl Indazolyl Thiadiazole, Triazole and Oxadiazole by Coventional and Nonconventional Methods
    SYNTHESIS AND CHARACTERIZATION OF SOME BIOLOGICALLY IMPORTANT 1-ISOPROPYL INDAZOLYL THIADIAZOLE, TRIAZOLE AND OXADIAZOLE BY COVENTIONAL AND NONCONVENTIONAL METHODS S.B. Kale, M.S. More and B.K.Karale* P.G. Department of Chemistry, S.S.G.M. College, Kopargaon, Ahmednagar - 423601 (M.S.), India e-mail: bkkarale@y ahoo. com Abstract: Compound 1 on treatment with SOCl2 followed by hydrazine hydrate gave acid hydrazide 2. Variously substituted phenyl isothicyanates with acid hydrazide 2 gave thiosemicarbazides 3. These thiosemicarbazides 3 on treatment with Cone. H2S04 and dil. NaOH gave thiadiazoles 4 and triazoles 5 respectively. Compound 3 on treatment with I2 in KI, in presence of NaOH gives oxadiazole 6. Introduction According to the literature survey, indazole compounds are associated with various physiological and biological properties and thus find important use in medicine. Indazole compounds are capable of mediating tyrosine kinase signal transduction and their by inhibit unwanted cell proliferation1,2. Indazole derivatives are examined for analgesic-anti-inflammatory activity3. A ruthenium co-ordination complex (Rulnd) is one the most effective anticancer4 ruthenium compound; poisoning5 of Topoisomerase II by indazole complex is analysed. Indazole ring was used as the initial template to test the hypothesis in order to increase potency as Leukotriene receptor antagonists6, 7' 8.Indazole containing inhibitor series for SAH/MTA nucleosidase are inhibitors with broad spectrum antimicrobial activity9. Indazole derivatives are used as anti-inflammatory agents10, anticancer10,11 agents and also used as sunscreens12. Thiosemicarbazide are found to be associated with antibacterial13, antifungal14 herbicidal15, antiacetyl Cholinesterase16 and antituburcular17 activities. Compounds containing 1,3,4-thiadiazole nucleus have been reported to a variety of biological activities like fungitoxic18, CNS stimulant19,anticholinergic20, hypoglycemia21, and anticonvulsant22,23.
    [Show full text]
  • Synthesis and Bioactivity of Hydrazide-Hydrazones with the 1-Adamantyl-Carbonyl Moiety
    molecules Article Synthesis and Bioactivity of Hydrazide-Hydrazones with the 1-Adamantyl-Carbonyl Moiety Van Hien Pham 1 , Thi Phuong Dung Phan 2, Dinh Chau Phan 3,* and Binh Duong Vu 1,* 1 Drug R&D Center, Vietnam Military Medical University. No.160, Phung Hung str., Phuc La ward, Ha Dong district, Hanoi 100000, Vietnam; [email protected] 2 Department of Pharmaceutical Chemistry, Hanoi University of Pharmacy. No. 15, Le Thanh Tong Str., Hoan Kiem district, Hanoi 100000, Vietnam; [email protected] 3 Hanoi University of Science and Technology. No.1, Dai Co Viet str., Bach Khoa ward, Hai Ba Trung district, Hanoi 100000, Vietnam * Correspondence: [email protected] (D.C.P.); [email protected] (B.D.V.); Tel.: +84-983-425-460 (B.D.V.); Fax: +84-243-688-4077 (B.D.V.) Academic Editor: Simona Collina Received: 3 October 2019; Accepted: 4 November 2019; Published: 5 November 2019 Abstract: Reaction of 1-adamantyl carbohydrazide (1) with various substituted benzaldehydes and acetophenones yielded the corresponding hydrazide-hydrazones with a 1-adamantane carbonyl moiety. The new synthesized compounds were tested for activities against some Gram-negative and Gram-positive bacteria, and the fungus Candida albicans. Compounds 4a, 4b, 5a, and 5c displayed potential antibacterial activity against tested Gram-positive bacteria and C. albicans, while compounds 4e and 5e possessed cytotoxicity against tested human cancer cell lines. Keywords: adamantane derivatives; hydrazide-hydrazone; antimicrobial; cytotoxicity activity 1. Introduction The hydrazide-hydrazones derivatives, which play an important role in organic and medicine chemistry, have attracted a large number of researchers over the years because of their promising biological activities, including antimicrobial [1–4], anticancer [5–7], antituberculosis [2,8–10], antiviral [11], and anticonvulsant [2] activities.
    [Show full text]
  • Synthesis and Biological Evaluation of Fatty Hydrazides of By-Products of Oil Processing Industry
    www.ijpsonline.com TABLE 2: SAMPLE CALCULATION FOR WEIGHT (WT) TABLE 3: SIMILARITY FACTORS FOR DIFFERENT TEST FOR TEST FORMULATION1 FORMULATIONS Time R T SDR SDT CVR CVT wt Test formulations f2-m f2-m3 f2 (min) 1 51.34 54.68 60.04 30 34.92 40.34 2.26 4.10 6.47 10.16 1.83 2 45.01 46.88 51.08 60 59.50 67.15 3.85 6.34 6.47 9.44 2.59 3 41.86 48.30 51.19 90 79.27 87.01 5.12 4.76 6.45 5.47 2.19 4 37.38 46.46 50.07 180 95.08 97.73 6.14 1.48 6.45 1.51 1.79 5 42.05 44.98 48.05 R = reference, T = Test , SDR = standard deviation of Rt, SDT = standard deviation f2-m = Similarity factor calculated using new approach, f2-m3 = Similarity factor of Tt, CVR = percentage coefficient of variation of Rt, CVT = percentage calculated using approach 3, f2 = Similarity factor calculated using conventional coefficient variation of tT , wt = 1 + (%CV of Rt/MCVE/L) + (% CV of Tt/MCVE/L) method (wt = 1) average value of reference and test at all the time point in Abbreviated New Drug Application (ANDA) point is similar then it is irrational to compute weight submission. If the value of f2-m is greater than 50 for calculating similarity factor because the product than we may safely conclude that products show similar dissolution. The positive and negative points of weight (wt) and (Rt-Tt) will be zero.
    [Show full text]
  • UC Berkeley UC Berkeley Previously Published Works
    UC Berkeley UC Berkeley Previously Published Works Title Synthesis of well-defined polyethylene-polydimethylsiloxane-polyethylene triblock copolymers by diimide-based hydrogenation of polybutadiene blocks Permalink https://escholarship.org/uc/item/7dk4d4pb Journal Macromolecules, 47(13) ISSN 0024-9297 Authors Petzetakis, N Stone, GM Balsara, NP Publication Date 2014-07-08 DOI 10.1021/ma500686k Peer reviewed eScholarship.org Powered by the California Digital Library University of California Article pubs.acs.org/Macromolecules Synthesis of Well-Defined Polyethylene−Polydimethylsiloxane− Polyethylene Triblock Copolymers by Diimide-Based Hydrogenation of Polybutadiene Blocks † ‡ † § Nikos Petzetakis, Gregory M. Stone, and Nitash P. Balsara*, , † Department of Chemical & Biomolecular Engineering, University of California, Berkeley, California 94720, United States ‡ Malvern Instruments Inc., 117 Flanders Road, Westborough, Massachusetts 01581, United States § Materials Sciences Division & Environmental Energy Technologies Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, California 94720, United States *S Supporting Information ABSTRACT: Polyethylene, PE, is a crystalline solid with a relatively high melting temperature, and it exhibits excellent solvent resistance at room temperature. In contrast, polydimethylsiloxane, PDMS, is a rubbery polymer with an ultralow glass transition temperature and poor solvent resistance. PE−PDMS block copolymers have the potential to synergistically combine these disparate properties.
    [Show full text]
  • Synthesis and Trypanocidal Activity of Salicylhydrazones and P-Tosylhydrazones of S-(+)-Carvone and Arylketones on African Trypanosomiasis
    Journal of Applied Pharmaceutical Science Vol. 5 (06), pp. 001-007, June, 2015 Available online at http://www.japsonline.com DOI: 10.7324/JAPS.2015.50601 ISSN 2231-3354 Synthesis and trypanocidal activity of salicylhydrazones and p-tosylhydrazones of S-(+)-carvone and arylketones on African trypanosomiasis Bienvenu GLINMA 1.2.4, Fernand A. GBAGUIDI 1.2.3*, Urbain C. KASSEHIN 3, Salomé D.S. KPOVIESSI1, Alban HOUNGBEME 2, Horrhus D. HOUNGUE3, Georges C. ACCROMBESSI 1 and Jacques H. POUPAERT3 1Laboratoire de Chimie Organique Physique et de Synthèse, Département de Chimie, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, 01 BP 4521 Cotonou, République Bénin. 2Laboratoire de Pharmacognosie/Institut de Recherche et d’Expérimentation en Médecine et Pharmacopée Traditionnelles (IREMPT) / Centre Béninois de la Recherche Scientifique et Technique (CBRST)/ UAC, 01 BP 06 Oganla Porto-Novo. 3Laboratoire de Chimie Pharmaceutique Organique, Ecole de Pharmacie, Faculté des Sciences de la Santé, Université d'Abomey-Calavi, Campus du Champ de Foire, 01 BP 188, Cotonou, Bénin. 4Louvain Drug Research Institute (LDRI), School of Pharmacy, Université Catholique de Louvain, B1 7203 Avenue Emmanuel Mounier 72, B-1200 Brussels, Belgique. ABSTRACT ARTICLE INFO Article history: Hydrazones are nowadays considered to be good candidates for various pharmaceutical applications. Here, we Received on: 06/03/2015 have synthesized two series of hydrazones: salicylhydrazones (GS1-4) and p-tosylhydrazones (GT1-4) from S- Revised on: 09/04/2015 (+)-carvone and three aryketones with good yields (57-91%). Molecules were characterized by elemental Accepted on: 22/04/2015 analyses; TLC, NMR 1H, NMR 13C and MS. Submitted, in vitro, to their antiparasitic testing on Trypanosoma Available online: 27/06/2015 brucei brucei, and toxicity on Artemia salina Leach, all compounds except GT2 showed significant antitrypanosomal activity IC50 ranging from 1 to 34 micromolar (µM).
    [Show full text]
  • Photo- and Acid-Degradable Polyacylhydrazone–Doxorubicin
    polymers Article Photo- and Acid-Degradable Polyacylhydrazone– Doxorubicin Conjugates Maria Psarrou 1, Martha Georgia Kothri 2 and Maria Vamvakaki 1,3,* 1 Department of Materials Science and Technology, University of Crete, Vasilika Vouton, 700 13 Heraklion, Crete, Greece; [email protected] 2 School of Medicine, University of Crete, Vasilika Vouton, 700 13 Heraklion, Crete, Greece; [email protected] 3 Institute of Electronic Structure and Laser, Foundation for Research and Technology-Hellas, Vasilika Vouton, 700 13 Heraklion, Crete, Greece * Correspondence: [email protected]; Tel.: +30-2810-545019 Abstract: Light-mediated polymer degradation has attracted considerable attention in various applications, including photo-patterning, tissue engineering and photo-triggered drug delivery. In this study, we report the synthesis and characterization of a new, linear, main-chain photo- and acid- degradable copolymer based on acylhydrazone linkages. The polymer was synthesized via a step- growth copolymerization of adipic acid dihydrazide with a bifunctional poly(ethylene glycol) bearing benzaldehyde end-groups, under mild acidic conditions, to afford a hydrophilic PEG-alt-adipic acid (PEG-alt-AA) alternating copolymer. The synthesized polymer was characterized by size exclusion chromatography, proton nuclear magnetic resonance and attenuated total reflection-Fourier transform infrared spectroscopies. The main-chain photo- and acid-induced degradation of the copolymer in dimethylsulfoxide and water, respectively, was verified by UV-vis spectroscopy at light intensities Citation: Psarrou, M.; Kothri, as low as 0.1 mW cm−2 at λ = 254 nm. Next, a model anticancer drug, doxorubicin (DOX), was M.G.; Vamvakaki, M. Photo- and Acid-Degradable Polyacylhydrazone– chemically linked to the polymer chain end(s) via acylhydrazone bond(s), resulting in amphiphilic Doxorubicin Conjugates.
    [Show full text]