bioRxiv preprint doi: https://doi.org/10.1101/410670; this version posted September 8, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 1 Quantification of protein mobility and associated reshuffling of 2 cytoplasm during chemical fixation 3 4 5 Authors: 6 Jan Huebinger *, Jessica Spindler, Kristin J. Holl, Björn Koos 7 8 9 Affiliations 10 Department of Systemic Cell Biology, Max Planck Institute of Molecular Physiology, 11 Otto-Hahn-Str.11, 44227 Dortmund, Germany 12 13 * Correspondence to:
[email protected] 14 15 16 1 bioRxiv preprint doi: https://doi.org/10.1101/410670; this version posted September 8, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 17 Abstract 18 19 To understand cellular functionalities, it is essential to unravel spatio-temporal 20 patterns of molecular distributions and interactions within living cells. The 21 technological progress in fluorescence microscopy now allows in principle to measure 22 these patterns with sufficient spatial resolution. However, high resolution imaging 23 comes along with long acquisition times and high phototoxicity. Physiological live 24 cell imaging is therefore often unfeasible and chemical fixation is employed. 25 However, fixation methods have not been rigorously reviewed to preserve patterns at 26 the resolution at which they can be nowadays imaged.