Polyclonal Antibody to PSMB4 / PROS26 (230-239) - Aff - Purified

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AP21517PU-N Polyclonal Antibody to PSMB4 / PROS26 (230-239) - Aff - Purified

Alternate names: HsBPROS26, HsN3, Macropain beta chain, Multicatalytic endopeptidase complex beta chain, PROS-26, Proteasome beta chain, Proteasome chain 3, Proteasome subunit beta type-4 Quantity: 0.1 mg Concentration: 0.5 mg/ml Background: The proteasome is a multicatalytic proteinase complex with a highly ordered ring- shaped 20S core structure. The core structure is composed of 4 rings of 28 non- identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Uniprot ID: P28070 NCBI: NP_002787.2 GeneID: 5692 Host: Goat Immunogen: Peptide with sequence (230-239) from the internal region of the protein sequence according to NP_002787.2. Genename: PSMB4 AA Sequence: C-NRFQIATVTE

Format: State: Liquid purified IgG fraction Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide Buffer System: Tris saline, pH~7.3 Preservatives: 0.02% Sodium Azide Stabilizers: 0.5% BSA Applications: Peptide ELISA: Detection limit: 1/8000. Western blot: 0.1-0.3 µg/ml. Approx 25-26kDa band observed in lysates of cell lines HEK293, HeLa, and K562 and in Mouse and Rat Liver lysates. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: Recognizes PSMB4 (aa 230-239). Species Reactivity: Tested: Human, Mouse, Rat. Expected from sequence similarity: Canine (Dog), Bovine, Rabbit. Add. Information: Calculated Molecular Weight: 29.1kDa (NP_002787.2).

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20130815 1 / 2 AP21517PU-N: Polyclonal Antibody to PSMB4 / PROS26 (230-239) - Aff - Purified

Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. General Readings: 1. Yoshida T, Kato K, Yokoi K, Oguri M, Watanabe S, Metoki N, Yoshida H, Satoh K, Aoyagi Y, Nozawa Y, Yamada Y, Association of genetic variants with hemorrhagic stroke in Japanese individuals. International journal of molecular medicine 2010 Apr 25 (4): 649-56. PMID: 20198315 Pictures: AP21517PU-N PSMB4 antibody staining of Mouse Liver lysate at 0.3 µg/ml (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

AP21517PU-N PSMB4 antibody staining of HEK293 lysate at 0.1 µg/ml (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20130815 2 / 2