TCIRG1 Cat. No.: 6973

TCIRG1 Antibody

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human, Mouse, Rat

TCIRG1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human TCIRG1. IMMUNOGEN:

The immunogen is located within amino acids 70 - 120 of TCIRG1.

TESTED APPLICATIONS: ELISA, WB

TCIRG1 antibody can be used for detection of TCIRG1 by Western blot at 0.5 μg/mL.

APPLICATIONS: Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.

At least two alternatively spliced isoforms are known to exist; this antibody will only detect SPECIFICITY: the larger isoform.

POSITIVE CONTROL: 1) Cat. No. 1287 - EL4 Lysate

PREDICTED MOLECULAR 91 kDa WEIGHT:

Properties

October 1, 2021 1 https://www.prosci-inc.com/tcirg1-antibody-6973.html PURIFICATION: TCIRG1 Antibody is affinity chromatography purified via peptide column.

CLONALITY: Polyclonal

ISOTYPE: IgG

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: TCIRG1 Antibody is supplied in PBS containing 0.02% sodium azide.

CONCENTRATION: 1 mg/mL

TCIRG1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one STORAGE CONDITIONS: year. As with all care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

OFFICIAL SYMBOL: TCIRG1

TCIRG1 Antibody: a3, Stv1, Vph1, Atp6i, OC116, OPTB1, TIRC7, ATP6N1C, ATP6V0A3, ALTERNATE NAMES: OC-116kDa, V-type proton ATPase 116 kDa subunit a isoform 3, Osteoclastic 116 kDa subunit, V-ATPase 116 kDa isoform a3

ACCESSION NO.: NP_006010

PROTEIN GI NO.: 19924145

GENE ID: 10312

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

TCIRG1 Antibody: The T-cell immune regulator 1 (TCIRG1) is the a3 subunit of the vacuolar-type proton transporting ATPase (V-ATPase), a proton pump in late endosomes and lysosomes that functions in the luminal acidification of these organelles. Genetic BACKGROUND: defects in the encoding this a responsible for a severe form of autosomal recessive , a condition that leads to increased bone density, decreased bone strength, and in bone tissues. TCIRG1 is also required for the normal secretion of insulin and the bacteria-killing function of macrophages.

1) Toyomura T, Oka T, Yamaguchi C, et al. Three subunit a isoforms of mouse vacuolar H+- REFERENCES: ATPase. Preferential expression of the a3 isoform during osteoclast differentiation. J. Biol. Chem. 2000; 275:8760-5

2) Kornak U, Schulz A, Friedrich W, et al. Mutations in the a3 subunit of the vacuolar H+- ATPase cause malignant osteopetrosis. Hum. Mol. Genet. 2000; 9:2059-63.

3) Sun-Wada GH, Toyomura T, Kawamura N, et al. The a3 isoform of V-ATPase regulates insulin secretion from pancreatic b-cells. J. Cell Sci. 2006; 119:4531-40.

4) Sun-Wada GH, Tabata H, Kawamura N, et al. Direct recruitment of H+-ATPase from lysosomes for phagocytic acidification. J. Cell Sci. 2009; 122:2504-13.

October 1, 2021 2 https://www.prosci-inc.com/tcirg1-antibody-6973.html ANTIBODIES FOR RESEARCH USE ONLY.

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October 1, 2021 3 https://www.prosci-inc.com/tcirg1-antibody-6973.html