Application of Limnology for Efficient Nutrient Utilization in Tropical Pond Aquaculture

t3 Verh. Internat. Verein. Limnol. 24 254 1- 2543 Stuttgart, September 1991

Application of limnology for efficient nutrient utilization in tropical pond aquaculture

C. F. Knud-Hansen, C. D. McNabb and T. R. Batterson

Introduction morning dissolved oxygen concentrations become growth limiting (Fig. 1). For optimal fish yields, maxi- Phosphorus, nitrogen and carbon have been key limit- mum food availability must be balanced with favorable ing nutrients on the forefront of eutrophication litera- pond water quality. This paper examines the role of ture for more than four decades. Concepts developed by nitrogen limitation in managing pond eutrophication in the limnological community regarding these nutrients order to greater and more predictable fish and their cycles in aquatic environments are now be- yields. coming the basis for management of aquaculture systems, particularly those in which fertilizers are used to promote fish production. Animal wastes, with a long Methods tradition of use in tropical aquaculture, represent an in- expensive source of organic carbon for detrital produc- Nitrogen input and fish yield data utilized in this anal- tion through heterotrophic pathways, and soluble nitro- ysis came from research conducted in Indonesia and gen and phosphorus for primary productivity. Depend- Thailand (for site description and analytical methods see ing on the species, fish will feed directly on attached or EGNAet al. 1987), and from other related published in- planktonic algae, detrital/fungal flocs, or smaller ani- vestigations. In all studies, Nile tilapia (Oreoch~omisnilo- mals such as zooplankton and snails which feed on algae ticus), either alone or in polyculture with carp (com- and detritus (COLMAN& EDWARDS1987). mon, silver and/or grass carp), were raised for 4-5 In most waste-fed systems, water quality degradation months in earthen ponds (surface areas of 0.02 - 0.04 ha, ultimately limits net fish yields (FY). Often as fertiliza- mean depths approximately 1m) with 2 to 4 replicates tion rates increase, inefficient nitrogen utilization to- per treatment-. All ponds received either fresh or aged gether with daytime pHs exceeding 9.0 results in high (> 1 week) chicken manure; some ponds also received unionized ammonia concentrations which reduce fish inorganic N as ammonium sulphate or urea. Fertilizers growth and survival (COLT81. ARMSTRONG1981, RUFFIER were applied daily, weekly, or biweekly depending on et al. 1981, MEADE1985). FY generally increases until the study. Daily mean total nitrogen loadings ranged high unionized ammonia concentrations and/or low from 0 - 0.70 g . m2,except for one treatment of 1.92 g . m-2.

Results Results showed that at nitrogen input rates below 0.2 g - m-2 . d-', fresh manure produced greater fish ~ieldsthan aged manure (Fig. 2). As N loading from fresh manure exceeded about 0.4 g . m-2 . d- I, FY decreased. FY surpassed 3.0 g fresh wt . m-2 . d-' when inorganic N was added in combination with manure at about 0.7g . m-2 . d-', or about three times the yield from fresh manure alone at equivalent N input. Regression analysis gave quadratic relationships between FY versus fresh manure loading (y = 0.72 + 3.08~- 11.01x2, Increasing Nutrient Input - r2 = 0.62, p < 0.1) and manure + inorganic N Fig. 1. Schematic representation of fish yield (FY) versus (y=0.06+6.80~-3.66x2, r2=0.94, p<0.01). nutrient input where FY is limited by low dissolved oxy- The relationship between FY and aged manure gen and/or high unionized ammonia concentrations. was significantly linear (y = 0.06 + 4.52x,

0368-0770/9 1/0024-2541 $0.75 1991 E Siharlzerbarr'5che Verlagsbuchhandlung D-7C00Sruttgart 1 2542 XI. Fish amd Fisheries

5.0- Fresh Manure equation, maximum FY would be 4.1 (k 0.3, - Aged Manure 1SE)g . m-2 . d'at N loading rate of 1.15g . 40- 0 Aged Manure + Inorganic N m-z . d-'. Light limitation from high algal densi- N' N' E \ ties, however, would probably prevent complete 30- N utilization at such high N input rates. At fertilization rates of at least 1.9eN" . m-2 . d-'. water quality degradation may result in high and unpre- dictable fish mortalities. Because efficient ammonia utilization reduces the risk of high" unionized ammonia concentrations, nitrogen limitation is desirable. Tropical 0.2 0.4 0 6 0 8 I0 freshwaters, often naturally limited by nitrogen Nitrogen Input (g/rn2/d alone or nitrogen in combination with phos- Fig.2. Yield of Nile tilapia (and in polyculture (PC) phorus (Moss 1969, ZARETet al. 198 1, MELACKet al. where indicated) versus nitrogen input from chicken 1982, SETARO& MELACK1984), can be made more manure which was added fresh (W), aged (O),or aged and nitrogen limited by the addition of manures (Table added with inorganic fertilizers (0).Numbers beside I), which generally have N: P ratios well below data points indicate source: 1 - this study; 2 - HOPKINSthe 7: 1 ratio by weight required by algae (RED- & CRUZ1982 (PC); 3 - GREENet al. 1989; 4 - MOAVet FIELD et al. 1963). When P fertilization exceeds algal al. 1977 (PC); 5 - SCHROEDER&BUCK 1987 (PC). " requirements, additional nitrogen input can increase fish vields (BOYD1976). Additional P in~ut is necessary to maintain N limitation in ponds

rZ = 0.91, p <0.01) because fertilization rates built in acid sul~hatesoils. common in the tro~ics.L, were still relatively low. The regression equation because of the soil's P sequestering capabilities for chicken manure plus inorganic N suggested (GAVIRIA,1986, unpubl. data). FY of 5 g . m-2 . that maximum fish productivity may reach over d ' or greater may be possible by increasing 4.0g - m-2 . d-' when total nitrogen input is organic and inorganic N input while maintaining about 0.7-0.8 g . m2 d-I. In 2 of the 4ponds re- N limitation through" effective addition of other ceiving 1.92gN . m-2 . d-' unionized ammonia- nutrients like P. Pond aeration to reduce nocturnal N levels surpassed 2 mg . IFL,and all the fish died oxygen deficits may further promote fish yields. after three months; this treatment was not in- In many tropical Asian countries, where fish re- cluded in the regression. mesent over 50 % of the total animal rotei in con- sumed and yields of wild fish are dwindling from Discussion overfishing and environmental degradation, increasing food production through fish cultivation Higher FY observed with fresh versus aged man- is the primary strategy for managing aquatic re- ure at lower fertilization rates probably resulted sources (EDWARDS1983). 1985 data from Thailand from the higher percentage of soluble N in fresh show that 36,500 pond/paddy fish farms totalling manure. Diminished FY at higher fresh manure 13,000ha produced 46,000 metric tons with a loading rates coincided with low dissolved oxygen commercial value of US $50.8 million (Dept. of concentrations at dawn, and increased manure-de- Fisheries 1987). Better understanding" of lim- rived turbidity which limited primary productiv- nological processes such as nutrient cycling, sed- ity and caused high concentrations of unionized iment/water interactions and trophic dynamics ammonia from reduced algal utilization (HOPKINS & CRUZ1982). In organically fertilized systems, detritus from manure decomposition may be an important die- Table 1. Representative nitrogen (N) and phosphorus (P) concentrations (as dry wt) of different manures used tary component for Nile tilapia (NORIEGA-CURTISfor pond fertilization.

1979. GREENet al. 1989). But bv substituting'J much of the manure-N with inorganic N, photosynthe- Manure % N % P N: P ratio Source tic production was increased. Ponds were gener- Chicken 1.4 2.2 0.6: 1 This study ally quite green (chlorophyll a concentrations ex- Cow 1.5 0.6 2.5: 1 GREENet al. 1989 ceeding 400 mg . m-3), and self-shading probably Duck 4.4 1.1 4.0: 1 A. I. T. 1986 limited algal productivity. Based on the regression Buffalo 1.4 0.2 7.0: 1 A. I. T. 1986 C. F. Knud-Hansen et al., Application of limnology for nutrient utilization 2543 should help determine optimal fertilization and GAVIRIA,J. I., 1986: Acid sulphate soils: identification, waste recycling schemes, thereby aiding develop- formation and implications for aquaculture. - J ing nations to produce food with greater ecol- Aqua. Trop. 1: 99 - 109. ogical and financial efficiency. GREEN,B. W., PHELPS,R. P. & ALVARARENGA,H. R., 1989: The effect of manures and chemical fertilizers on the production of Oveocbromis niloticus in earthen ponds. - Aquaculture 76: 37- 42. Acknowledgements HOPKINS,K. D. & CRUZ,E. M., 1982: The ICLARM- The research was conducted jointly by Michigan State CLSU integrated animal-fish farming project: University (U.S.A.), the Institut Pertanian Bogor (Indo- final report. - ICLARM Tecbn. Reports 5: 96 pp. nesia), and the Department of Fisheries (Thailand) as a Internat. Center for Living Aquatic Resources part of the Pond Dynamics/Aquaculture Collaborative Management, Philippines. Research Support Program (CRSP) supported by the MEADE,J. W., 1985: Allowable ammonia for fish culture. Agency for International Development, Grant No. - Prog. Fish-Cult. 47 (3): 135- 145. DAN-4023-GSS-7066-00 and by contributions from the MELACK,J. M., KILHAM,P. & FISHER,T. R., 1982: Re- participating institutions. Assistance from the Michigan sponses of phytoplankton to experimental ferti- State University Agricultural Experiment Station is lization with ammonium and phosphate in an acknowledged. This paper is Pond Dynamics/Aquacul- African soda lake. - Oecologia 52: 321 - 326. ture CRSP publication number 89: 37. MOAV,R., WOHLFARTH,G., SCHROEDER,G. L., HULATA, G. & BARASH,H., 1977: Intensive polyculture of fish in freshwater ponds. I. Substitution of ex- References pensive feeds by liquid cow manure. - Aquacul- ture 10: 25-43. A. I. T., 1986: Buffalo/fish and duck/fish integrated sys- Moss, B., 1969: Limitation of algal growth in some Cen- tems for small-scale farmers at the family level. - tral African waters. - 14: Asian Inst. of Technol. Research Rep. No. 198, A. I. Limnol. Oceanogr. 591-601. T., Bangkok, Thailand, 63 pp. NORIEGA-CURTIS,P., 1979: Primary productivity and re- BARASH,H., PLAVNIK,I. & MOAV,R., 1982: Integration of lated fish yield in intensely manured fishponds. - duck and fish farming: experimental results. - Aquaculture 17: 335 -344. Aquaculture 27: 129 - 140. REDFIELD,A. C., KETCHUM,B. H. & RICHARDS,F. A,, BOYD,C. E., 1976: Nitrogen fertilizer effects on produc- 1963: The influence of organisms on the composi- tion of Tilapia in ponds fertilized with phos- tion of sea water. - In: fie Sea, HILLM. A. (Ed.), phorus and potassium. - 7: 385 - 390. AquacuLture Vol. 2: 27-28. - Interscience Publ. New York. COLMAN,J. & EDWARDS,P., 1987: Feeding pathways RUFFIER,P. J., BOYLE,W. C. & KLEINSCHMIDT,J., 1981: and environmental constraints in waste-fed Short-term acute bioassays to evaluate ammonia aquaculture: balance and optimization. - In: toxicity and effluent standards. - J. Water Poll. MORIARTY,D. J. W. & PULLIN,R. S. V. (Eds.), De- Contr. Fed. 53: 367 - 377. tritus and Microbial Ecology in Aquaculture: 240-281. - ICLARM Conf. Proc. 14, Internat. SCHROEDER,G. L. & BUCK,H., 1987: Estimates of the rel- ative contributions of organic and mineral con- Center for Living Aquatic Resources Manage- tents of manure to fish growth. - J. Aqua. Trop. 2: ment, Manila, Philippines. 133 - 138. COLT,J. E. & ARMSTRONG,D. A,, 1981: Nitrogen toxi- SETARO,F. V. & MELACK,J. M., 1984: Responses of city to crustaceans, fish, and molluscs. - In: phytoplankton to experimental nutrient enrich- ALLEN,L. & KINNEY, C. (Eds.), J. E. Proc. Bio-en- ment in an Amazon floodplain lake. - Lzmnol. gineering Symp. for Fish Culture: 34 -47. - Fish Oceanogr. 29 (5):972 - 984. Culture Section, Amer. Fish. Soc., Bethesda, MD. ZARET,T. M., DEVOL,A. H. & SANTOS,A. D., 1981: NU- Department of Fisheries, 1987: Freshwater fishfarm trient addition experiments in Lago Jacaretinga, production 1985. - Report No. 6/1987. Dept. of Central Amazon Basin, Brasil. - Verb. Internat. Fisheries, Bangkok, Thailand. Verein. Limnol. 21: 721 - 724. EDWARDS,P., 1983: The future potential of integrated farming systems in Asia. - Proc. I/ WCAP 1: 273-281. Authors' addresses: EGNA,H. S., BROWN,N. & LESLIE,M., 1987: Pond C. F. KNUD-HANSEN,Asian Institute of Technol- Dynamics/Aquaculture Collaborative Research ogy, Agr. and Food Engineering, G. P.O. Box Data Reports. Vol. 1. General Reference: Site De- 2754, Bangkok 10501, Thailand. scriptions, Materials and Methods for the Global C. D. MCNABBand T. R. BATTERSON,Department Experiment. - Oregon State Univ., Corvallis, of Fisheries and Wildlife, Michigan State Univer- 84 PP. sity, East Landing, Michigan 48824, USA. 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