Meat, Fish, Poultry & Eggs TEACHER EDITION your answer.) Lab Question 5. 4. 3. 2. 1. In thislab,youwilllearnhowto… Lab Objectives which proteinsarecomplementary. amino acids. You will be asked to consider which proteins are complete proteins and, if not complete, In this investigation, you will be constructing animal and plant proteins using beads to represent the Lab Overview Name: ______STUDENT LAB INVESTIGATIONS The BuildingBlocks Food ExplorationsLabI: Which ofthefollowingfoodsourcesarecomplete proteins(containallessentialaminoacids)?(Circle Explain therelevanceofcomplementaryproteinsinavegetariandiet. Explain theeffectofinsufficientessentialaminoacidsonbody’sproteinsynthesis. Identify completeandincompleteproteins. Construct amodelprotein. Describe therelationshipofdietaryproteinandhealthmajorbodysystems. Meat

Peanuts

• White Rice and Beans

Toast determine whichcoloredbead(s)representseachaminoacid. their abbreviationsarelistedonthefollowingpage.Withdirectionfromyourteacher,create a keyto each adifferentcolor.Eachcoloredbeadwillrepresentsingleaminoacid.The22acids and In thisinvestigationyouwillbeworkingwithcoloredbeads.Yourgroupgiven4bagsof beads, PROCEDURE 4 CraftPipeCleaners 4 bagsofAminoAcids(coloredbeads) MATERIALS Building Proteins 2. ______isacompleteproteinbecause______1. ______isacompleteproteinbecause______Predictions: ______I predictthefollowingtwofoodsareconsideredcompleteproteins: • • TEACHER EDITION

Meat, Fish, Poultry & Eggs Meat, Fish, Poultry & Eggs 2. 1. TEACHER EDITION Abbreviation: Leucine Abbreviation: Isoleucine Abbreviation: Histidine Abbreviation: Glycine Abbreviation: Glutamic Acid Abbreviation: Glutamine Abbreviation: Cysteine Abbreviation: Aspartic Abbreviation: Asparagine Abbreviation: Arginine Abbreviation: Alanine Using apipecleaner If youdeterminearemissinganaminoacid, writetheword“missing”besidebeadcolor. acids inorder ofusefrom1to22. once, soyouhaveatotal of44beadsonyourstrand.Inthepreceding chart,numbertheamino Glycine, Methionine,Proline, Threonine,Tyrosine,Selenocysteine,andPyrrolysine. Repeatthisorder Asparagine, Tryptophan, Lysine, Leucine, Phenylalanine, Cysteine, Aspatric Acid, Arginine, Glutamine, each foodproteinfollowing thisorder:Histidine,GlutamicAcid,Valine, Alanine,Serine,Isoleucine, acid. When youareconstructingyourproteinstrand, useaclearbeadtopresentthe“missing”amino Acid Leu Ile His Gly Gln Cys Asp Asn Agr Ala Glu

Bead-Color: ______Bead-Color: ______Bead-Color: ______Bead-Color: ______Bead-Color: ______Bead-Color: ______Bead-Color: ______Bead-Color: ______Bead-Color: ______Bead-Color: ______Bead-Color: ______to organizetheaminoacidsinaprimarystructure (straight chain),create Amino AcidBead-ColorKey Abbreviation: Threonine Abbreviation: Serine Abbreviation: Proline Abbreviation: Phenylalanine Abbreviation: Methionine Abbreviation: Pyrrolysine Abbreviation: Selenocysteine Abbreviation: Valine Abbreviation: Tyrosine Abbreviation: Tryptophan Abbreviation: Lysine Thr Ser Pro Phe Met Pyl Se-CYs Val Tyr Trp Lys

Bead-Color: ______Bead-Color: ______Bead-Color: ______Bead-Color: ______Bead-Color: ______Bead-Color: ______Bead-Color: ______Bead-Color: ______Bead-Color: ______Bead-Color: ______Bead-Color: ______3. Data: Missing AminoAcid(s): GluValAlaSerIleAspTryLysLeuPheCys—ArgGinGlyMetProThrTyrSePylPly Missing Amino Acid(s):______Missing AminoAcid(s):______Missing AminoAcid(s):______Missing AminoAcid(s):______Label theaminoacidswithineachproteinmoleculebelow(seeexamplebelow).Whenapplicable, identify thelocationandname(s)ofanymissingaminoacids. do notfalloff. NOTE: Afteraddingthefirstfewaminoacidsbendendofpipecleanerslightlytoensurebeads Aspartic Acid(Asp) White RiceandBeanProtein Protein MoleculeDrawings Example Protein Peanut Protein Toast Protein Meat Protein TEACHER EDITION

Meat, Fish, Poultry & Eggs Meat, Fish, Poultry & Eggs 3. 2. 1. Conclusion: TEACHER EDITION In anyproteinsthatwerenotcomplete,whichaminoacidslimiting? Compare andcontrastthecompleteproteinsconstructedtoincompleteconstructed. Describe whichofthefourfoodsource(s)areconsideredcomplementaryproteins. complete protein. amino acids.When combined, the in the missing amino acidsarefoundothertomake a Rice andbeans are complementary proteins because they are each missing different combined, they would also make a complementary protein. Rice and beans combine to make a complementary protein.Iftoast and peanuts were Toast and peanuts are missing essential amino acids, while rice andbeans are not. Peanuts andtryptophan. are missingmethionineToast is missinglysine and isoleucine. a is considered complementary combination protein. all oftheaminoacids;this beans contains Theof riceand protein. combination incomplete proteins. Meat contains all of the amino acids, and is therefore a complete Peanuts toast and do notcontainall the essential aminoacids, and aretherefore 6. 5. 4. Describe theimpactonbody’sabilitytomakeproteinsifaperson’sdietislowin Why shouldindividualsconsumingaplant-baseddiet(e.g.vegetarians)understandcomplementary Using examplesofmajorbodysystems,explaintheimportanceconsumingcompletesources of sources ofprotein? protein forthebody’shealth. containing essentialaminoacids. proteins. promoting musclefoodsaretheonlysourceofcomplete growth andrepair.Animal acids tocomplete organsandbones, necessary includingmaintaining functions, They complementary should understand proteinsbecause our bodiesneedall 22 amino tissues thosecontaining aminoacids (i.e.forenergy). for utilization acids. Withoutto breakdown essential amino acids,ourbodieswillbegin Consuming complete sources of proteinis important to provide ourbodieswithall the is body the acids, amino essential the unable tomake enough protein tosupport cell structure andgrowth. containing proteins in low is diet person’s a If TEACHER EDITION

Meat, Fish, Poultry & Eggs