Comprehensive GCMS and LC-MS/MS Metabolite Profiling of Chlorella Vulgaris
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Meta Gene 2 (2014) 746–760
Meta Gene 2 (2014) 746–760 Contents lists available at ScienceDirect Meta Gene Characterization of the bovine gene LIPE and possible influence on fatty acid composition of meat Daniel Estanislao Goszczynski a,c, Juliana Papaleo Mazzucco b, María Verónica Ripoli a, Edgardo Leopoldo Villarreal b, Andrés Rogberg-Muñoz a, Carlos Alberto Mezzadra b, Lilia Magdalena Melucci b,⁎, Guillermo Giovambattista a,⁎⁎ a IGEVET, CCT LA PLATA CONICET, FCV, UNLP, La Plata B1900AVW, CC 296, Argentina b Unidad Integrada INTA Balcarce — FCA, UNMdP, Argentina c Fellow of the Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Argentina article info abstract Article history: LIPE is an intracellular neutral lipase, which is capable of hydrolyzing a va- Received 10 June 2014 riety of esters and plays a key role in the mobilization of fatty acids from Revised 26 August 2014 diacylglycerols. The objectives of this study were to characterize the Accepted 3 September 2014 genetic polymorphism of bovine LIPE gene and to evaluate the possible Available online 16 October 2014 association between three SNPs in the coding regions of this gene with the fatty acid composition of meat in a cattle population. Forty-three unre- Keywords: lated animals from different cattle breeds were re-sequenced and 21 SNPs LIPE fi Polymorphism were detected over approximately 2600 bp, ve of these SNPs were novel. Bovine Three SNPs were selected, on the basis of evolutionary conservation, to Lipid content perform validation and association studies in a crossbred cattle popula- tion. Our results may suggest a possible association of SNP1 with contents of oleic acid and total monounsaturated fatty acids (p b 0.01), and SNP2 and SNP3 with Heneicosylic acid content (p b 0.01), may be helpful to improve the quality of meat and improve health. -
(12) United States Patent (10) Patent No.: US 9,375.433 B2 Dilly Et Al
US009375433B2 (12) United States Patent (10) Patent No.: US 9,375.433 B2 Dilly et al. (45) Date of Patent: *Jun. 28, 2016 (54) MODULATORS OF ANDROGENSYNTHESIS (52) U.S. Cl. CPC ............. A6 IK3I/519 (2013.01); A61 K3I/201 (71) Applicant: Tangent Reprofiling Limited, London (2013.01); A61 K3I/202 (2013.01); A61 K (GB) 31/454 (2013.01); A61K 45/06 (2013.01) (72) Inventors: Suzanne Dilly, Oxfordshire (GB); (58) Field of Classification Search Gregory Stoloff, London (GB); Paul USPC .................................. 514/258,378,379, 560 Taylor, London (GB) See application file for complete search history. (73) Assignee: Tangent Reprofiling Limited, London (56) References Cited (GB) U.S. PATENT DOCUMENTS (*) Notice: Subject to any disclaimer, the term of this 5,364,866 A * 1 1/1994 Strupczewski.......... CO7C 45/45 patent is extended or adjusted under 35 514,254.04 U.S.C. 154(b) by 0 days. 5,494.908 A * 2/1996 O’Malley ............. CO7D 261/20 514,228.2 This patent is Subject to a terminal dis 5,776,963 A * 7/1998 Strupczewski.......... CO7C 45/45 claimer. 514,217 6,977.271 B1* 12/2005 Ip ........................... A61K 31, 20 (21) Appl. No.: 14/708,052 514,560 OTHER PUBLICATIONS (22) Filed: May 8, 2015 Calabresi and Chabner (Goodman & Gilman's The Pharmacological (65) Prior Publication Data Basis of Therapeutics, 10th ed., 2001).* US 2015/O238491 A1 Aug. 27, 2015 (Cecil's Textbook of Medicine pp. 1060-1074 published 2000).* Stedman's Medical Dictionary (21st Edition, Published 2000).* Okamoto et al (Journal of Pain and Symptom Management vol. -
WO 2017/074902 Al 4 May 20 17 (04.05.2017) W P O P C T
(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2017/074902 Al 4 May 20 17 (04.05.2017) W P O P C T (51) International Patent Classification: AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, A61K 8/37 (2006.01) A61Q 19/00 (2006.01) BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, A61K 31/215 (2006.01) DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, (21) International Application Number: KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, PCT/US2016/058591 MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, (22) International Filing Date: OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, 25 October 2016 (25.10.201 6) SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, (25) Filing Language: English ZW. (26) Publication Language: English (84) Designated States (unless otherwise indicated, for every (30) Priority Data: kind of regional protection available): ARIPO (BW, GH, 62/247,803 29 October 20 15 (29. 10.20 15) US GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, (71) Applicant: GLAXOSMITHKLINE CONSUMER TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, HEALTHCARE HOLDINGS (US) LLC [US/US]; 271 1 DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, Centerville Road, Suite 400, Wilmington, DE 19808 (US). -
Altered Xanthophyll Compositions Adversely Affect Chlorophyll Accumulation and Nonphotochemical Quenching in Arabidopsis Mutants
Proc. Natl. Acad. Sci. USA Vol. 95, pp. 13324–13329, October 1998 Plant Biology Altered xanthophyll compositions adversely affect chlorophyll accumulation and nonphotochemical quenching in Arabidopsis mutants BARRY J. POGSON*, KRISHNA K. NIYOGI†,OLLE BJO¨RKMAN‡, AND DEAN DELLAPENNA§¶ *Department of Plant Biology, Arizona State University, Tempe, AZ 85287-1601; †Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720-3102; ‡Department of Plant Biology, Carnegie Institution of Washington, Stanford, CA 94305-4101; and §Department of Biochemistry, University of Nevada, Reno, NV 89557-0014 Contributed by Olle Bjo¨rkman, September 4, 1998 ABSTRACT Collectively, the xanthophyll class of carote- thin, are enriched in the LHCs, where they contribute to noids perform a variety of critical roles in light harvesting assembly, light harvesting, and photoprotection (2–8). antenna assembly and function. The xanthophyll composition A summary of the carotenoid biosynthetic pathway of higher of higher plant photosystems (lutein, violaxanthin, and neox- plants and relevant chemical structures is shown in Fig. 1. anthin) is remarkably conserved, suggesting important func- Lycopene is cyclized twice by the enzyme lycopene b-cyclase tional roles for each. We have taken a molecular genetic to form b-carotene. The two beta rings of b-carotene are approach in Arabidopsis toward defining the respective roles of subjected to identical hydroxylation reactions to yield zeaxan- individual xanthophylls in vivo by using a series of mutant thin, which in turn is epoxidated once to form antheraxanthin lines that selectively eliminate and substitute a range of and twice to form violaxanthin. Neoxanthin is derived from xanthophylls. The mutations, lut1 and lut2 (lut 5 lutein violaxanthin by an additional rearrangement (9). -
What Pigments Are in Plants?
BUILD YOUR FUTURE! ANYANG BEST COMPLETE MACHINERY ENGINEERING CO.,LTD WHAT PIGMENTS ARE IN PLANTS? Pigments Pigments are chemical compounds responsible for color in a range of living substances and in the inorganic world. Pigments absorb some of the light they receive, and so reflect only certain wavelengths of visible light. This makes them appear "colorful.” Cave paintings by early man show the early use of pigments, in a limited range from straw color to reddish brown and black. These colors occurred naturally in charcoals, and in mineral oxides such as chalk and ochre. The WebExhibit on Pigments has more information on these early painting palettes. Many early artists used natural pigments, but nowadays they have been replaced by cheaper and less toxic synthetic pigments. Biological Pigments Pigments are responsible for many of the beautiful colors we see in the plant world. Dyes have often been made from both animal sources and plant extracts . Some of the pigments found in animals have also recently been found in plants. Website: www.bestextractionmachine.com Email: [email protected] Tel: +86 372 5965148 Fax: +86 372 5951936 Mobile: ++86 8937276399 BUILD YOUR FUTURE! ANYANG BEST COMPLETE MACHINERY ENGINEERING CO.,LTD Major Plant Pigments White Bird Of Paradise Tree Bilirubin is responsible for the yellow color seen in jaundice sufferers and bruises, and is created when hemoglobin (the pigment that makes blood red) is broken down. Recently this pigment has also been found in plants, specifically in the orange fuzz on seeds of the white Bird of Paradise tree. The bilirubin in plants doesn’t come from breaking down hemoglobin. -
Abscisic Acid Induced Protection Against Photoinhibition of PSII Correlates with Enhanced Activity of the Xanthophyll Cycle
FEBS 15944 FEBS Letters 371 (1995) 61-64 Abscisic acid induced protection against photoinhibition of PSII correlates with enhanced activity of the xanthophyll cycle A.G. Ivanov a'*, M. Krol b, D. Maxwell b, N.P.A. Huner b alnstitute of Biophysics, Bulgarian Academy of Sciences, Aead. (7. Bonchev Street, bl. 21, 1113 Sofia, Bulgaria bDepartment of Plant Sciences, University of Western Ontario, London, Ont. N6A 5B7, Canada Received 22 June 1995; revised version received 24 July 1995 applied ABA on the light-dependent zeaxanthin formation, the Abstract The exogenous application of abscisic acid (ABA) to related capacity for non-photochemical chlorophyll fluores- barley seedlings resulted in partial protection of the PSII photo- cence quenching and the possible involvement of ABA in the chemistry against photoinhibition at low temperature, the effect protection of PSII photochemistry from excessive radiation at being most pronounced at 10 -s M ABA. This was accompanied low temperatures. by higher photochemical quenching (qP) in ABA-treated leaves. A considerable increase (122%) in the amount of total carotenoids 2. Materials and methods and xanthophylls (antheraxanthin, violaxanthin and zeaxanthin) was also found in the seedlings subjected to ABA. The activity Seeds of barley (Hordeum vulgare L. var. cadette) were germinated of the xanthophyll cycle measured by the epoxidation state of for 3 days and grown in aqueous solutions of ABA (10 -5 M and 10-6 M) xanthophyUs under high-light treatment was higher in ABA- as in [23]. ABA treatments lasted 7 days. The solutions were changed treated plants compared with the control. This corresponds to a daily. -
Genetic Modification of Tomato with the Tobacco Lycopene Β-Cyclase Gene Produces High Β-Carotene and Lycopene Fruit
Z. Naturforsch. 2016; 71(9-10)c: 295–301 Louise Ralley, Wolfgang Schucha, Paul D. Fraser and Peter M. Bramley* Genetic modification of tomato with the tobacco lycopene β-cyclase gene produces high β-carotene and lycopene fruit DOI 10.1515/znc-2016-0102 and alleviation of vitamin A deficiency by β-carotene, Received May 18, 2016; revised July 4, 2016; accepted July 6, 2016 which is pro-vitamin A [4]. Deficiency of vitamin A causes xerophthalmia, blindness and premature death, espe- Abstract: Transgenic Solanum lycopersicum plants cially in children aged 1–4 [5]. Since humans cannot expressing an additional copy of the lycopene β-cyclase synthesise carotenoids de novo, these health-promoting gene (LCYB) from Nicotiana tabacum, under the control compounds must be taken in sufficient quantities in the of the Arabidopsis polyubiquitin promoter (UBQ3), have diet. Consequently, increasing their levels in fruit and been generated. Expression of LCYB was increased some vegetables is beneficial to health. Tomato products are 10-fold in ripening fruit compared to vegetative tissues. the most common source of dietary lycopene. Although The ripe fruit showed an orange pigmentation, due to ripe tomato fruit contains β-carotene, the amount is rela- increased levels (up to 5-fold) of β-carotene, with negli- tively low [1]. Therefore, approaches to elevate β-carotene gible changes to other carotenoids, including lycopene. levels, with no reduction in lycopene, are a goal of Phenotypic changes in carotenoids were found in vegeta- plant breeders. One strategy that has been employed to tive tissues, but levels of biosynthetically related isopre- increase levels of health promoting carotenoids in fruits noids such as tocopherols, ubiquinone and plastoquinone and vegetables for human and animal consumption is were barely altered. -
Biochemistry Prologue to Lipids
Paper : 05 Metabolism of Lipids Module: 01 Prologue to Lipids Principal Investigator Dr. Sunil Kumar Khare, Professor, Department of Chemistry, IIT-Delhi Paper Coordinator and Dr. Suaib Luqman, Scientist (CSIR-CIMAP) Content Writer & Assistant Professor (AcSIR) CSIRDr. Vijaya-CIMAP, Khader Lucknow Dr. MC Varadaraj Content Reviewer Prof. Prashant Mishra, Professor, Department of Biochemical Engineering and Biotechnology, IIT-Delhi 1 METABOLISM OF LIPIDS Biochemistry Prologue to Lipids DESCRIPTION OF MODULE Subject Name Biochemistry Paper Name 05 Metabolism of Lipids Module Name/Title 01 Prologue to Lipids 2 METABOLISM OF LIPIDS Biochemistry Prologue to Lipids 1. Objectives To understand what is lipid Why they are important How they occur in nature 2. Concept Map LIPIDS Fatty Acids Glycerol 3. Description 3.1 Prologue to Lipids In 1943, the term lipid was first used by BLOOR, a German biochemist. Lipids are heterogeneous group of compounds present in plants and animal tissues related either actually or potentially to the fatty acids. They are amphipathic molecules, hydrophobic in nature originated utterly or in part by thioesters (carbanion-based condensations of fatty acids and/or polyketides etc) or by isoprene units (carbocation-based condensations of prenols, sterols, etc). Lipids have the universal property of being: i. Quite insoluble in water (polar solvent) ii. Soluble in benzene, chloroform, ether (non-polar solvent) 3 METABOLISM OF LIPIDS Biochemistry Prologue to Lipids Thus, lipids include oils, fats, waxes, steroids, vitamins (A, D, E and K) and related compounds, such as phospholipids, triglycerides, diglycerides, monoglycerides and others, which are allied more by their physical properties than by their chemical assests. -
Natural Colour Book
THE COLOUR BOOK Sensient Food Colors Europe INDEX NATURAL COLOURS AND COLOURING FOODS INDEX 46 Lycopene 4 We Brighten Your World 47 Antho Blends – Pink Shade 6 Naturally Different 48 Red Cabbage 8 The Colour of Innovation 49 Beetroot – with reduced bluish tone 10 Natural Colours, Colouring Foods 50 Beetroot 11 Cardea™, Pure-S™ 51 Black Carrot 12 YELLOW 52 Grape 14 Colourful Impulses 53 Enocianin 15 Carthamus 54 Red Blends 16 Curcumin 56 VIOLET & BLUE 17 Riboflavin 59 Violet Blends 18 Lutein 61 Spirulina 19 Carrot 62 GREEN 20 Natural Carotene 65 Green Blends 22 Beta-Carotene 66 Copper-Chlorophyllin 24 Annatto 67 Copper-Chlorophyll 25 Yellow/ Orange Blends 68 Chlorophyll/-in 26 ORANGE 69 Spinach 29 Natural Carotene 70 BROWN 30 Paprika Extract 73 Burnt Sugar 32 Carrot 74 Apple 33 Apocarotenal 75 Caramel 34 Carminic Acid 76 BLACK & WHITE 35 Beta-Carotene 79 Vegetable Carbon 36 RED 80 Titanium Dioxide 39 Antho Blends – Strawberry Shade 81 Natural White 40 Aronia 41 Elderberry 83 Regulatory Information 42 Black Carrot 84 Disclaimer 43 Hibiscus 85 Contact Address 44 Carmine 3 INDEX NATURAL COLOURS AND COLOURING FOODS WE BRIGHTEN YOUR WORLD Sensient is as colourful as the world around us. Whatever you are looking for, across the whole spectrum of colour use, we can deliver colouring solutions to best meet your needs in your market. Operating in the global market place for over 100 years Sensient both promises and delivers proven international experience, expertise and capabilities in product development, supply chain management, manufacture, quality management and application excellence of innovative colours for food and beverages. -
Studies on Betalain Phytochemistry by Means of Ion-Pair Countercurrent Chromatography
STUDIES ON BETALAIN PHYTOCHEMISTRY BY MEANS OF ION-PAIR COUNTERCURRENT CHROMATOGRAPHY Von der Fakultät für Lebenswissenschaften der Technischen Universität Carolo-Wilhelmina zu Braunschweig zur Erlangung des Grades einer Doktorin der Naturwissenschaften (Dr. rer. nat.) genehmigte D i s s e r t a t i o n von Thu Tran Thi Minh aus Vietnam 1. Referent: Prof. Dr. Peter Winterhalter 2. Referent: apl. Prof. Dr. Ulrich Engelhardt eingereicht am: 28.02.2018 mündliche Prüfung (Disputation) am: 28.05.2018 Druckjahr 2018 Vorveröffentlichungen der Dissertation Teilergebnisse aus dieser Arbeit wurden mit Genehmigung der Fakultät für Lebenswissenschaften, vertreten durch den Mentor der Arbeit, in folgenden Beiträgen vorab veröffentlicht: Tagungsbeiträge T. Tran, G. Jerz, T.E. Moussa-Ayoub, S.K.EI-Samahy, S. Rohn und P. Winterhalter: Metabolite screening and fractionation of betalains and flavonoids from Opuntia stricta var. dillenii by means of High Performance Countercurrent chromatography (HPCCC) and sequential off-line injection to ESI-MS/MS. (Poster) 44. Deutscher Lebensmittelchemikertag, Karlsruhe (2015). Thu Minh Thi Tran, Tamer E. Moussa-Ayoub, Salah K. El-Samahy, Sascha Rohn, Peter Winterhalter und Gerold Jerz: Metabolite profile of betalains and flavonoids from Opuntia stricta var. dilleni by HPCCC and offline ESI-MS/MS. (Poster) 9. Countercurrent Chromatography Conference, Chicago (2016). Thu Tran Thi Minh, Binh Nguyen, Peter Winterhalter und Gerold Jerz: Recovery of the betacyanin celosianin II and flavonoid glycosides from Atriplex hortensis var. rubra by HPCCC and off-line ESI-MS/MS monitoring. (Poster) 9. Countercurrent Chromatography Conference, Chicago (2016). ACKNOWLEDGEMENT This PhD would not be done without the supports of my mentor, my supervisor and my family. -
Survey of Plant Pigments: Molecular and Environmental Determinants of Plant Colors
ACTA BIOLOGICA CRACOVIENSIA Series Botanica 51/1: 7–16, 2009 SURVEY OF PLANT PIGMENTS: MOLECULAR AND ENVIRONMENTAL DETERMINANTS OF PLANT COLORS EWA MŁODZIŃSKA* Department of Plant Physiology, Wroclaw University, ul. Kanonia 6/8, 50-328 Wrocław, Poland Received January 7, 2009; revision accepted February 20, 2009 It is difficult to estimate the importance of plant pigments in plant biology. Chlorophylls are the most important pigments, as they are required for photosynthesis. Carotenoids are also necessary for their functions in photosyn- thesis. Other plant pigments such as flavonoids play a crucial role in the interaction between plants and animals as visual signals for pollination and seed scattering. Studies related to plant pigmentation are one of the oldest areas of work in plant science. The first publication about carotenoids appeared in the early nineteenth century, and the term "chlorophyll" was first used in 1818 (Davies, 2004). Since then, the biochemical structure of plant pigments has been revealed, as have the biosynthetic pathways for the major pigments that provide a useful variety of colors to blossoms and other plant organs. There is widespread interest in the application of molecular methods to improve our knowledge of gene regulation mechanisms and changes in plant pigment content. Genetic modification has been used to alter pigment production in transgenic plants. This review focuses on flower pigmentation, its bio- chemistry and biology. It presents a general overview of the major plant pigment groups as well as rarer plant dyes and their diversity and function in generating the range of colors observed in plants. Key words: Flower and fruit colors, co-pigmentation, plant dyes, pigment groups. -
Graham Centre Monograph No. 4
Long-chain omega-3 polyunsaturated fatty acids in ruminant nutrition: benefits to animals and humans Edward H. Clayton Livestock Research Officer – Ruminant Nutrition NSW Department of Primary Industries, Wagga Wagga Agricultural Institute Pine Gully Rd, Wagga Wagga NSW 2650 Graham Centre Monograph No. 4 Edited by: Toni Nugent and Catriona Nicholls August 2014 © State of New South Wales through Department of Trade and Investment, Regional Infrastructure and Services 2014 This publication is copyright. You may download, display, print and reproduce this material in an unaltered form only (retaining this notice) for your personal use or for non-commercial use within your organisation. To copy, adapt, publish, distribute or commercialise any of this publication you will need to seek permission from the NSW Department of Primary Industries. Disclaimer: The information contained in this publication is based on knowledge and understanding at the time of writing (August 2014). However, because of advances in knowledge, users are reminded of the need to ensure that information upon which they rely is up to date and to check currency of the information with the appropriate officer of the NSW Department of Primary Industries or the user’s independent advisor. All sources of information in the current publication are acknowledged in the text. No further reproduction should be made without first obtaining prior written approval of the copyright owner. For updates to this publication, check www.grahamcentre.net/ Published by the NSW Department of Primary Industries. First published August 2014 ISBN 978 1 74256 678 8 Cover design by: Sharon Kiss Cover photo by: Toni Nugent, Graham Centre for Agricultural Innovation Author’s Contact: Dr Edward Clayton, Livestock Research Officer, NSW Department of Primary Industries, Wagga Wagga Agricultural Institute, Pine Gully Rd, Wagga Wagga NSW 2650 Email: [email protected] Citation: Clayton EH (2014).