The Olfactory Receptor Associated Proteome
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Human Anatomy (Biology 2) Lecture Notes Updated July 2017 Instructor
Human Anatomy (Biology 2) Lecture Notes Updated July 2017 Instructor: Rebecca Bailey 1 Chapter 1 The Human Body: An Orientation • Terms - Anatomy: the study of body structure and relationships among structures - Physiology: the study of body function • Levels of Organization - Chemical level 1. atoms and molecules - Cells 1. the basic unit of all living things - Tissues 1. cells join together to perform a particular function - Organs 1. tissues join together to perform a particular function - Organ system 1. organs join together to perform a particular function - Organismal 1. the whole body • Organ Systems • Anatomical Position • Regional Names - Axial region 1. head 2. neck 3. trunk a. thorax b. abdomen c. pelvis d. perineum - Appendicular region 1. limbs • Directional Terms - Superior (above) vs. Inferior (below) - Anterior (toward the front) vs. Posterior (toward the back)(Dorsal vs. Ventral) - Medial (toward the midline) vs. Lateral (away from the midline) - Intermediate (between a more medial and a more lateral structure) - Proximal (closer to the point of origin) vs. Distal (farther from the point of origin) - Superficial (toward the surface) vs. Deep (away from the surface) • Planes and Sections divide the body or organ - Frontal or coronal 1. divides into anterior/posterior 2 - Sagittal 1. divides into right and left halves 2. includes midsagittal and parasagittal - Transverse or cross-sectional 1. divides into superior/inferior • Body Cavities - Dorsal 1. cranial cavity 2. vertebral cavity - Ventral 1. lined with serous membrane 2. viscera (organs) covered by serous membrane 3. thoracic cavity a. two pleural cavities contain the lungs b. pericardial cavity contains heart c. the cavities are defined by serous membrane d. -
The Pattern of Olfactory Innervation by W
J Neurol Neurosurg Psychiatry: first published as 10.1136/jnnp.9.3.101 on 1 July 1946. Downloaded from THE PATTERN OF OLFACTORY INNERVATION BY W. E. LE GROS CLARK and R. T. TURNER WARWICK From the Department of Anatomy, University of Oxford (RECEIVED 31ST JULY, 1946) IT is desirable that, from time to time, commonly Methods accepted statements regarding anatomical pathways Most of the observations recorded in this paper were and connexions in the peripheral and central nervous made on rabbit material. The fixation of the olfactory systems should be carefully reviewed in the light of mucosa presented considerable difficulty. The method modern technical methods of investigation, for it finally selected, because it gave the best results with must be admitted that not a few of these statements protargol and was also adequate for the other stains are based on old methods which are now recognized employed, was perfusion of 70 per cent. alcohol through to be too crude to permit of really accurate con- the aorta, after preliminary washing through with normal clusions. In recent years, indeed, a number of saline, as recommended by Bodian (1936). Another facts have been shown unexpected difficulty arose from the fact that a large apparently well-established number of laboratory rabbits suffer from a chronic by critical studies to be erroneous. For example, rhinitis which leads to gross pathological changes in the the so-called ventral nucleus of the lateral geniculate olfactory mucosa. Consequently, a considerable pro- Protected by copyright. body and the pulvinar are no longer accepted as portion of our material, experimental and otherwise, terminal stations of the optic tract, and the strie had to be discarded as useless. -
Spontaneous Neural Activity Is Required for the Establishment and Maintenance of the Olfactory Sensory Map
Neuron, Vol. 42, 553–566, May 27, 2004, Copyright 2004 by Cell Press Spontaneous Neural Activity Is Required for the Establishment and Maintenance of the Olfactory Sensory Map C. Ron Yu,1,2 Jennifer Power,2 Gilad Barnea,2 mus and cortex, results from complementary gradients Sean O’Donnell,3 Hannah E.V. Brown,4 of ephrin receptors on retinal afferents and ephrins ex- Joseph Osborne,3 Richard Axel,2,3,4,* pressed by the multiple targets in the brain (Wilkinson, and Joseph A. Gogos2,5,* 2001). These retinotopic representations deconstruct 1Department of Anatomy and Cell Biology the visual scene into submaps that refine the image, 2 Center for Neurobiology and Behavior including ocular dominance columns and orientation 3 Department of Biochemistry columns, as well as cortical blobs that uniquely respond and Molecular Biophysics to color, motion, and spatial frequency (Van Essen et al., 4 Howard Hughes Medical Institute 1992). These refinements are sensitive to visual activity, 5 Department of Physiology and Cellular Biophysics and this has led to efforts to distinguish the relative College of Physicians and Surgeons contributions of innate determinants (timing of axon ar- Columbia University rival and molecular cues) and experiential or activity- New York, New York 10032 dependent mechanisms in the generation of the visual circuitry (Katz and Shatz, 1996). The problem is perhaps best illustrated by early recordings by Hubel and Wiesel Summary that demonstrated that cortical neurons respond prefer- entially to one or the other eye and segregate into ocular We have developed a genetic approach to examine dominance columns (LeVay et al., 1978; Wiesel and Hu- the role of spontaneous activity and synaptic release bel, 1963). -
Proteomic Atlas of the Human Olfactory Bulb
JOURNAL OF PROTEOMICS 75 (2012) 4005– 4016 Available online at www.sciencedirect.com www.elsevier.com/locate/jprot Proteomic atlas of the human olfactory bulb Joaquín Fernández-Irigoyena, Fernando J. Corralesb, Enrique Santamaríaa,⁎ aProteomics Unit, Biomedical Research Center (CIB), Navarra Health Service, 31008 Pamplona, Spain bProteomics Unit, Centre for Applied Medical Research (CIMA), University of Navarra, Pamplona, Spain ARTICLE INFO ABSTRACT Article history: The olfactory bulb (OB) is the first site for the processing of olfactory information in the Received 12 March 2012 brain and its deregulation is associated with neurodegenerative disorders. Although Accepted 7 May 2012 different efforts have been made to characterize the human brain proteome in depth, the Available online 15 May 2012 protein composition of the human OB remains largely unexplored. We have performed a comprehensive analysis of the human OB proteome employing protein and peptide Keywords: fractionation methods followed by LC-MS/MS, identifying 1529 protein species, correspond- Olfactory bulb ing to 1466 unique proteins, which represents a 7-fold increase in proteome coverage with Brain respect to previous OB proteome descriptions from translational models. Bioinformatic Proteomics analyses revealed that protein components of the OB participated in a plethora of biological Mass spectrometry process highlighting hydrolase and phosphatase activities and nucleotide and RNA binding Bioinformatics activities. Interestingly, 631 OB proteins identified were not previously described in protein datasets derived from large-scale Human Brain Proteome Project (HBPP) studies. In particular, a subset of these differential proteins was mainly involved in axon guidance, opioid signaling, neurotransmitter receptor binding, and synaptic plasticity. Taken together, these results increase our knowledge about the molecular composition of the human OB and may be useful to understand the molecular basis of the olfactory system and the etiology of its disorders. -
Expressing Exogenous Functional Odorant Receptors in Cultured Olfactory Sensory Neurons Huaiyang Chen1, Sepehr Dadsetan2, Alla F Fomina2 and Qizhi Gong*1
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Springer - Publisher Connector Neural Development BioMed Central Methodology Open Access Expressing exogenous functional odorant receptors in cultured olfactory sensory neurons Huaiyang Chen1, Sepehr Dadsetan2, Alla F Fomina2 and Qizhi Gong*1 Address: 1Department of Cell Biology and Human Anatomy, School of Medicine, University of California, Davis, California 95616, USA and 2Department of Physiology and Membrane Biology, School of Medicine, University of California, Davis, California 95616, USA Email: Huaiyang Chen - [email protected]; Sepehr Dadsetan - [email protected]; Alla F Fomina - [email protected]; Qizhi Gong* - [email protected] * Corresponding author Published: 11 September 2008 Received: 22 May 2008 Accepted: 11 September 2008 Neural Development 2008, 3:22 doi:10.1186/1749-8104-3-22 This article is available from: http://www.neuraldevelopment.com/content/3/1/22 © 2008 Chen et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Olfactory discrimination depends on the large numbers of odorant receptor genes and differential ligand-receptor signaling among neurons expressing different receptors. In this study, we describe an in vitro system that enables the expression of exogenous odorant receptors in cultured olfactory sensory neurons. Olfactory sensory neurons in the culture express characteristic signaling molecules and, therefore, provide a system to study receptor function within its intrinsic cellular environment. -
Odorant-Binding Protein: Localization to Nasal Glands and Secretions (Olfaction/Mucus/Immunohistochemistry/Pyrazines) JONATHAN PEVSNER, PAMELA B
Proc. Nail. Acad. Sci. USA Vol. 83, pp. 4942-4946, July 1986 Neurobiology Odorant-binding protein: Localization to nasal glands and secretions (olfaction/mucus/immunohistochemistry/pyrazines) JONATHAN PEVSNER, PAMELA B. SKLAR, AND SOLOMON H. SNYDER* Departments of Neuroscience, Pharmacology, and Experimental Therapeutics, Psychiatry, and Behavioral Sciences, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205 Contributed by Solomon H. Snyder, January 6, 1986 ABSTRACT An odorant-binding protein (OBP) was iso- formed at an antiserum dilution of 1:8100 in 0.1 M Tris HCl, lated from bovine olfactory and respiratory mucosa. We have pH 8.0/0.5% Triton X-100, in a final vol of50 A.l. Incubations produced polyclonal antisera to this protein and report its were carried out at 370C for 90 min with 35,000 cpm of immunohistochemical localization to mucus-secreting glands of '251-labeled OBP per tube. Immunoprecipitation was accom- the olfactory and respiratory mucosa. Although OBP was plished by using 25 1LI of 5% Staphylococcus aureus cells originally isolated as a pyrazine binding protein, both rat and (Calbiochem) in 0.1 M Tris HCl (pH 8.0) at 370C for 30 min. bovine OBP also bind the odorants [3H]methyldihydrojasmon- Bound 1251I-labeled OBP was separated from free OBP by ate and 3,7-dimethyl-octan-1-ol as well as 2-isobutyl-3-[3HI filtration over glass fiber filters (No. 32, Schleicher & methoxypyrazine. We detect substantial odorant-binding ac- Schuell) pretreated with 10% fetal bovine serum, using a tivity attributable to OBP in secreted rat nasal mucus and tears Brandel cell harvester (Brandel, Gaithersburg, MD). -
Genomics of Mature and Immature Olfactory Sensory Neurons Melissa D
University of Kentucky UKnowledge Physiology Faculty Publications Physiology 8-15-2012 Genomics of Mature and Immature Olfactory Sensory Neurons Melissa D. Nickell University of Kentucky, [email protected] Patrick Breheny University of Kentucky, [email protected] Arnold J. Stromberg University of Kentucky, [email protected] Timothy S. McClintock University of Kentucky, [email protected] Right click to open a feedback form in a new tab to let us know how this document benefits oy u. Follow this and additional works at: https://uknowledge.uky.edu/physiology_facpub Part of the Genomics Commons, and the Physiology Commons Repository Citation Nickell, Melissa D.; Breheny, Patrick; Stromberg, Arnold J.; and McClintock, Timothy S., "Genomics of Mature and Immature Olfactory Sensory Neurons" (2012). Physiology Faculty Publications. 66. https://uknowledge.uky.edu/physiology_facpub/66 This Article is brought to you for free and open access by the Physiology at UKnowledge. It has been accepted for inclusion in Physiology Faculty Publications by an authorized administrator of UKnowledge. For more information, please contact [email protected]. Genomics of Mature and Immature Olfactory Sensory Neurons Notes/Citation Information Published in Journal of Comparative Neurology, v. 520, issue 12, p. 2608-2629. Copyright © 2012 Wiley Periodicals, Inc. This is the peer reviewed version of the following article: Nickell, M. D., Breheny, P., Stromberg, A. J., and McClintock, T. S. (2012). Genomics of mature and immature olfactory sensory neurons. Journal of Comparative Neurology, 520: 2608–2629, which has been published in final form at http://dx.doi.org/ 10.1002/cne.23052. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. -
Chapter 17: the Special Senses
Chapter 17: The Special Senses I. An Introduction to the Special Senses, p. 550 • The state of our nervous systems determines what we perceive. 1. For example, during sympathetic activation, we experience a heightened awareness of sensory information and hear sounds that would normally escape our notice. 2. Yet, when concentrating on a difficult problem, we may remain unaware of relatively loud noises. • The five special senses are: olfaction, gustation, vision, equilibrium, and hearing. II. Olfaction, p. 550 Objectives 1. Describe the sensory organs of smell and trace the olfactory pathways to their destinations in the brain. 2. Explain what is meant by olfactory discrimination and briefly describe the physiology involved. • The olfactory organs are located in the nasal cavity on either side of the nasal septum. Figure 17-1a • The olfactory organs are made up of two layers: the olfactory epithelium and the lamina propria. • The olfactory epithelium contains the olfactory receptors, supporting cells, and basal (stem) cells. Figure 17–1b • The lamina propria consists of areolar tissue, numerous blood vessels, nerves, and olfactory glands. • The surfaces of the olfactory organs are coated with the secretions of the olfactory glands. Olfactory Receptors, p. 551 • The olfactory receptors are highly modified neurons. • Olfactory reception involves detecting dissolved chemicals as they interact with odorant-binding proteins. Olfactory Pathways, p. 551 • Axons leaving the olfactory epithelium collect into 20 or more bundles that penetrate the cribriform plate of the ethmoid bone to reach the olfactory bulbs of the cerebrum where the first synapse occurs. • Axons leaving the olfactory bulb travel along the olfactory tract to reach the olfactory cortex, the hypothalamus, and portions of the limbic system. -
Mechanisms of Ciliary Targeting of the Olfactory Cyclic Nucleotide- Gated Channel
Mechanisms of Ciliary Targeting of the Olfactory Cyclic Nucleotide- Gated Channel by Paul Michael Jenkins A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy (Pharmacology) in The University of Michigan 2010 Doctoral Committee: Associate Professor Jeffrey Randall Martens, Chair Professor Lori L. Isom Professor Benjamin L. Margolis Associate Professor Kristen J. Verhey © Paul Michael Jenkins All Rights Reserved 2010 To my family ii ACKNOWLEDGEMENTS I would like to first extend my sincerest gratitude to my research mentor, Dr. Jeffrey R. Martens. The time I have spent in his laboratory has shaped me both professionally and personally. His drive and dedication to science serve as a model that I strive towards every day. The past few years have been extremely rewarding for me due to his friendship, patience, and continual mentoring. I would not be where I am today without his guidance. I would also like to acknowledge my thesis committee members, Dr. Lori Isom, Dr. Ben Margolis and Dr. Kristen Verhey. I have been extremely lucky to have a committee that not only acted as thesis advisors, but also as collaborators, mentors, and friends. I would like to thank the numerous members of the Martens laboratory, present and former: Kristin Arendt, Dave Dudek, Nikhil Iyer, Sajida Jackson, Qiuju Li, Dyke McEwen, Jeremy McIntyre, Sarah Schumacher, Laurie Svoboda, Kristin van Genderen, Eileen Vesely, Tiffney Widner, Liz Williams, Kendra Yum, and Lian Zhang. Your antics in lab have kept me alternating -
A Role for STOML3 in Olfactory Sensory Transduction
Research Article: New Research | Sensory and Motor Systems A role for STOML3 in olfactory sensory transduction https://doi.org/10.1523/ENEURO.0565-20.2021 Cite as: eNeuro 2021; 10.1523/ENEURO.0565-20.2021 Received: 28 December 2020 Revised: 25 January 2021 Accepted: 8 February 2021 This Early Release article has been peer-reviewed and accepted, but has not been through the composition and copyediting processes. The final version may differ slightly in style or formatting and will contain links to any extended data. Alerts: Sign up at www.eneuro.org/alerts to receive customized email alerts when the fully formatted version of this article is published. Copyright © 2021 Agostinelli et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. A role for STOML3 in olfactory sensory transduction Abbreviated title: STOML3 in olfactory transduction Authors: Emilio Agostinelli1†, Kevin Y. Gonzalez-Velandia1†, Andres Hernandez-Clavijo1, Devendra Kumar Maurya1§, Elena Xerxa1, Gary R Lewin2, Michele Dibattista3‡, Anna Menini1‡, Simone Pifferi1,4‡ 1 Neurobiology Group, SISSA, Scuola Internazionale Superiore di Studi Avanzati, 34136 Trieste, Italy. 2 Molecular Physiology of Somatic Sensation, Department of Neuroscience, Max Delbrück Center for Molecular Medicine, D-13122 Berlin, Germany. 3 Department of Basic Medical Sciences, Neuroscience and Sensory -
Archivio Istituzionale Open Access Dell'università Di Torino Transitory
AperTO - Archivio Istituzionale Open Access dell'Università di Torino Transitory and activity-dependent expression of Neurogranin in olfactory bulb tufted cells during mouse postnatal development. This is the author's manuscript Original Citation: Transitory and activity-dependent expression of Neurogranin in olfactory bulb tufted cells during mouse postnatal development. / Gribaudo S.; Bovetti S.; Friard O.; Denorme M.; Oboti L.; Fasolo A.; De Marchis S.. - In: JOURNAL OF COMPARATIVE NEUROLOGY. - ISSN 1096-9861. - 520(2012), pp. 3055-3069. Availability: This version is available http://hdl.handle.net/2318/107513 since 2017-05-18T11:16:01Z Published version: DOI:10.1002/cne.23150 Terms of use: Open Access Anyone can freely access the full text of works made available as "Open Access". Works made available under a Creative Commons license can be used according to the terms and conditions of said license. Use of all other works requires consent of the right holder (author or publisher) if not exempted from copyright protection by the applicable law. (Article begins on next page) 25 September 2021 This is an author version of the contribution published on: Questa è la versione dell’autore dell’opera: [The Journal of Comparative Neurology, 520 (14), 2012, DOI: 10.1002/cne.23150] ovvero [Gribaudo S., Bovetti S., Friard O., Denorme M., Oboti L., Fasolo A., De Marchis S. 520 (14), Wiley, 2012, pagg.3055-3069] The definitive version is available at: La versione definitiva è disponibile alla URL: [http://onlinelibrary.wiley.com/doi/10.1002/cne.23150/abstract;jsessionid=DA945D 1BAD21815004ADF2F5441730B9.f02t02] Transitory and Activity-Dependent Expression of Neurogranin in Olfactory Bulb Tufted Cells During Mouse Postnatal Development S. -
Role of Human Papilloma Virus in the Etiology of Primary Malignant Sinonasal Tumors
ROLE OF HUMAN PAPILLOMA VIRUS IN THE ETIOLOGY OF PRIMARY MALIGNANT SINONASAL TUMORS A DISSERTATION SUBMITTED IN PARTIAL FULFILLMENT OF M.S BRANCH –IV (OTORHINOLARYNGOLOGY) EXAMINATION OF THE TAMILNADU DR.MGR MEDICAL UNIVERSITY TO BE HELD IN APRIL 2016 | P a g e ROLE OF HUMAN PAPILLOMA VIRUS IN THE ETIOLOGY OF PRIMARY MALIGNANT SINONASAL TUMORS A DISSERTATION SUBMITTED IN PARTIAL FULFILLMENT OF M.S BRANCH –IV (OTORHINOLARYNGOLOGY) EXAMINATION OF THE TAMILNADU DR.MGR MEDICAL UNIVERSITY TO BE HELD IN APRIL 2016 | P a g e CERTIFICATE This is to certify that the dissertation entitled ‘ROLE OF HUMAN PAPILLOMA VIRUS IN THE ETIOLOGY OF PRIMARY MALIGNANT SINONASAL TUMORS’ is a bonafide original work of Dr Miria Mathews, carried out under my guidance, in partial fulfilment of the rules and regulations for the MS Branch IV, Otorhinolaryngology examination of The Tamil Nadu Dr. M.G.R Medical University to be held in April 2016. Dr Rajiv Michael Guide Professor and Head of Unit – ENT 1 Department of ENT Christian Medical College Vellore | P a g e CERTIFICATE This is to certify that the dissertation entitled ‘ROLE OF HUMAN PAPILLOMA VIRUS IN THE ETIOLOGY OF PRIMARY MALIGNANT SINONASAL TUMORS’ is a bonafide original work of Dr Miria Mathews, in partial fulfilment of the rules and regulations for the MS Branch IV, Otorhinolaryngology examination of The Tamil Nadu Dr. M.G.R Medical University to be held in April 2016. Dr Alfred Job Daniel Dr John Mathew Principal Professor and Head Christian Medical College Department of Otorhinolaryngology Vellore Christian Medical College Vellore | P a g e CERTIFICATE This is to certify that the dissertation entitled ‘ROLE OF HUMAN PAPILLOMA VIRUS IN THE ETIOLOGY OF PRIMARY MALIGNANT SINONASAL TUMORS’ is a bonafide original work in partial fulfilment of the rules and regulations for the MS Branch IV, Otorhinolaryngology examination of The Tamil Nadu Dr.